CN104815060A - Traditional Chinese medicine composition for treating acute alcoholic liver injury - Google Patents

Abstract

The invention belongs to a novel purpose of a traditional Chinese medicine, and especially relates to an application of the traditional Chinese medicine for treating acute alcoholic liver injury. The traditional Chinese medicine composition comprises the following components by weight ratio: 150 parts of mint, 150 parts of Chinese magnoliavine, 150 parts of ligustrum lucidum, 250 parts of wolfberry, 250 parts of red sage root, and 780 parts of dextrin. A preparation method comprises the following steps: decocting 10 times of wolfberry according to formula amount for twice, wherein each time lasts for 1 hour, filtrating; adding 10 times of alcohol with concentration being 70% in mint, Chinese magnoliavine, ligustrum lucidum and red sage root and extracting for twice, wherein each time lasts for 1 hour, filtrating; merging a filtrate, concentrating to relative density (1.15-1.2), granulating with a proper amount of dextrin, drying, separating, taking blood to detect ALT and AST index, comparing the serum ALT and AST with a model group to find that serum ALT and AST can be obviously reduced (P is less than 0.05-0.001). mice serum ALT and AST of acute alcoholic liver injury is reduced, and the traditional Chinese medicine composition has certain prevention and protection effect for treating liver injury.

Description

A kind of Chinese medicine composition for the treatment of acute alcohol-induced hepatic injury

Technical field

The invention belongs to the novelty teabag of Chinese medicine, in particular for the purposes in acute alcohol-induced hepatic injury.

Background technology

Modern society, due to expanding economy, the communication of person to person is more and more frequent, often can have a meal by drinking and promote carrying out of commercial activity, thus unavoidably the damage of acute alcoholic liver occurs in a lot of occasion.Clinical frequent employing improves the medicine of symptom and biochemical index, as SAM, polyene phosphatidylcholine, glycyrrhizic acid preparation, silymarin-group and reduced glutathion etc.But, these medicines or expensive, or there is various side effect.

Summary of the invention

The technical problem that invention solves:

Use this precious resources of traditional medicine, overcome all drawbacks of acute liver damage present stage western medicine.

The technical scheme that invention adopts:

Treating a Chinese medicine composition for acute alcohol-induced hepatic injury, it is characterized by by weight being Herba Menthae 150 parts, Fructus Schisandrae Chinensis 150 parts, Fructus Ligustri Lucidi 150 parts, Fructus Lycii 250 parts, Radix Salviae Miltiorrhizae 250 parts, 780 parts, dextrin.Said composition body adopts following technique:

By formula ratio Fructus Lycii 10 times of soak by water 2 times, each 1 hour, filter; All the other raw material Herba Menthaes, Fructus Schisandrae Chinensis, Fructus Ligustri Lucidi, Radix Salviae Miltiorrhizae adds 10 times amount 70% alcohol extraction 2 times, each 1 hour, filters.Merging filtrate, is concentrated into relative density (1.15 ~ 1.2), granulates with appropriate dextrin, granulate, dry, subpackage.

Make granule 1000g (5g/ bag) altogether, this product using method and use amount are: every day 2 times, each 1 bag, sooner or later respectively once.

This combination object space solution:

Liver " body the moon sun ", for the maincenter of QI-blood circulation, chronic hepatitis convalescent period person, clinically morely shows as hepatopathy and spleen, hepatopathy violates stomach, Liver and kidney with the deficiency syndrome of disease, and has the stagnation of QI, blood stasis, the mark reality such as damp and hot concurrently, treatment should follow the instruction of " Medical Treasures of the Golden Chamber " " disease of liver; using drugs with sour taste for invigoration, help with burnt bitter, the medicine of using sweet drugs for tonifying is to adjust it ", " seeing the disease of liver; knowing the liver disease will be transmitted into the spleen, in the ban tonifying the spleen ".Facing card medication should be soft and moist, tonifying liver body and help liver use, therefore side's Fructus Lycii, Fructus Ligustri Lucidi are monarch's liver and kidney tonifying, Fructus Lycii sweet in the mouth, " Records of Tradition Chinese and Western Medicine in Combination ": " the many liquid of sweet in the mouth, slightly cool in nature are the medicine that nourishing the liver and kidney is the best; therefore its property is apt to improving eyesight; reducing the asthenic fever, and strengthening bone and muscle, except pain in the lumbar region; take useful for a long time, this all nourishing the liver and kidney merit also "; Fructus Ligustri Lucidi sweet-bitter flavor, " book on Chinese herbal medicine is taken advantage of refined ": " take then the spleen transporting essential substances to the liver for a long time, and excess of QI is in bones of the body collectively, fertile being good for is made light of one's life by commiting suicide always, and its outer note of the ancient Chinese is also ".Minister with Fructus Schisandrae Chinensis, Radix Salviae Miltiorrhizae, Fructus Schisandrae Chinensis sour in the mouth tonifying liver, Compendium of Material Medica: " acid salty enter liver and the kidney invigorating, pungent bitter in taste acting on the heart and tonifying the lung, sweet enter Middle Palace strengthening the spleen stomach "; Radix Salviae Miltiorrhizae nourishing blood and promoting blood circulation, " book on Chinese herbal medicine is just read ": " Radix Salviae Miltiorrhizae, merit with four things, can blood stasis dispelling with tissue regeneration promoting, kind wind and the eliminating stagnation treated, property is gentle and walk blood ... be medicine first of conditioning blood system ".Help soothing the liver with Herba Menthae acrid in the mouth, " herbal classic is met former ": " the pungent cool rising of Herba Menthae, enters liver, lung two warp, pungently can disperse ... its property is floating and rise, and is the order of spring liters in medicine, and energy dissipating depression of QI falls apart gas, therefore XIAOYAO POWDER with it ".The merit of liver and kidney tonifying, vital energy regualting and blood circulation-promoting played altogether by all medicines, also closes the purport of " Medical Treasures of the Golden Chamber " " help with burnt bitter, the medicine of using sweet drugs for tonifying is to adjust it for the disease of liver, using drugs with sour taste for invigoration ".

The beneficial effect of the invention:

For acute liver damage patient provides a kind of good and cheap, Chinese medicine safely and efficiently.

Detailed description of the invention

1. material

1.1 animals: cleaning grade ICR kind mice, body weight 18 ~ 20g, ♀ ♂ half and half, is purchased from Shanghai Slac Experimental Animal Co., Ltd.'s (former Chinese Academy of Sciences Shanghai Experimental Animal Center), production licence number: SCXK (Shanghai) 2012-0002; Animal feeding environment: cleaning grade, occupancy permit number: SYXK (Soviet Union) 2011-0016.

1.2 test samples: Chinese medicine composition extract of the present invention (hereinafter referred to as ethanol side), preparation technology is:

By formula ratio Fructus Lycii 10 times of soak by water 2 times, each 1 hour, filter; All the other raw material Herba Menthaes, Fructus Schisandrae Chinensis, Fructus Ligustri Lucidi, Radix Salviae Miltiorrhizae adds 10 times amount 70% alcohol extraction 2 times, each 1 hour, filters.Merging filtrate, is concentrated into relative density (1.15 ~ 1.2), granulates with appropriate dextrin, granulate, dry, subpackage.

This ethanol side is containing raw medicinal herbs 3.03g/ml, and lot number: 131018, Jiangsu Prov. Research Inst. Traditional Chinese Medical natural resources of Chinese medicinal materials and chemical research room provide.

1.3 reagent: dehydrated alcohol, normal saline etc.

2. experimental technique

2.1 animal groupings, administration: get ICR mice and be divided into 5 groups at random, often organize 10, ♀ ♂ half and half, be respectively alcohol liver disease model group (normal saline 20ml/kg, ig), ethanol side's heavy dose (2.4g crude drug/kg, ig, is about 4 times that intend clinical people's dose,equivalent), dosage (0.6 crude drug/kg in ethanol side, ig, intend clinical people's dose,equivalent), ethanol side's low dose (0.15g crude drug kg, ig, be about and intend 1/4 of clinical people's dose,equivalent) and Normal group (normal saline 2ml/kg, ig).After medicine is mixed with finite concentration by distilled water, all by 20ml/ (kgd) gavage (ig) administration, successive administration 2 weeks, measures Mouse Weight weekly.

2.2 mice alcohol liver disease models make: each administration treated animal was successive administration 2 weeks, 1h after last medicine, and after simultaneously fasting can't help water 12h, by the dosage of 10ml/kg body weight disposable gavage 50% dehydrated alcohol, continue fasting and can't help water, taken a blood sample by orbital venous plexus after 6h and survey ALT, AST.

3. result

3.1 ethanol sides are on the impact of acute alcohol-induced hepatic injury mice ALT, AST: (see table 1)

Table 1 ethanol side affects acute alcohol-induced hepatic injury mice ALT, AST

Group	Dosage	ALT(u/L)	AST(u/L)
					(g crude drug/kg)
Normal group	20mlNS	46.0±10.0***	284.6±90.8
				Model group	20mlNS	75.5±18.1	336.7±67.8
Heavy dose of group	2.4	61.5±12.3	356.1±88.9
				Middle dosage group	0.6	66.3±22.2	281.9±119.9

[0026]

Small dose group

0.15

41.4±11.5***

259.7±59.6*

Note: compare with model group: *: P < 0.05; * *: P<0.001.

4. sum up

After each administration group mouse stomach 50% ethanol, occur walking unstable, the phenomenon such as to run chaotically in cage.After 6h, in blood, ALT value model group is apparently higher than Normal group, and ALT, AST of small dose group animal are starkly lower than model group (P<0.001), maintains an equal level with normal control treated animal; Dosage group big or middle also has certain reduction to become, but without statistical significant difference, there is the effect significantly reducing ALT, AST the ethanol side of display doses to acute alcohol-induced hepatic injury.Result shows, the ethanol side of suitable dose has clear and definite protective effect to acute alcohol-induced hepatic injury.

Claims (1)

1. treat a Chinese medicine composition for acute alcohol-induced hepatic injury, it is characterized by by weight being Herba Menthae 150 parts, Fructus Schisandrae Chinensis 150 parts, Fructus Ligustri Lucidi 150 parts, Fructus Lycii 250 parts, Radix Salviae Miltiorrhizae 250 parts, 780 parts, dextrin, its preparation method is:

A, by formula ratio Fructus Lycii 10 times of soak by water 2 times, each 1 hour, to filter;

B, all the other raw material Herba Menthaes, Fructus Schisandrae Chinensis, Fructus Ligustri Lucidi, Radix Salviae Miltiorrhizae adds 10 times amount 70% alcohol extraction 2 times, each 1 hour, filters;

C, merging filtrate, be concentrated into relative density (1.15 ~ 1.2), granulate with appropriate dextrin, granulate, dry.