CN104782594B - Compound attractant for simultaneously attracting female moths and male moths of chilo suppressalis and application thereof - Google Patents
Compound attractant for simultaneously attracting female moths and male moths of chilo suppressalis and application thereof Download PDFInfo
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- NPNUFJAVOOONJE-ZIAGYGMSSA-N β-(E)-Caryophyllene Chemical compound C1CC(C)=CCCC(=C)[C@H]2CC(C)(C)[C@@H]21 NPNUFJAVOOONJE-ZIAGYGMSSA-N 0.000 claims abstract description 30
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- NPNUFJAVOOONJE-UONOGXRCSA-N caryophyllene Natural products C1CC(C)=CCCC(=C)[C@@H]2CC(C)(C)[C@@H]21 NPNUFJAVOOONJE-UONOGXRCSA-N 0.000 claims abstract description 15
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Abstract
A composite attractant for simultaneously attracting female moths and male moths of chilo suppressalis and application thereof. The invention discloses a Chilo suppressalis attractant and application thereof, wherein the Chilo suppressalis attractant is composed of a sex attractant and a host odor composition, wherein the host odor composition is composed of the following components in parts by weight: 2-4 parts of limonene, 12-18 parts of zingiberene, 12-18 parts of caryophyllene and 60-74 parts of trans-2-hexenal. The chilo suppressalis sex attractant is used in combination with the host odor composition, and the synergistic attraction effect is achieved. The Chilo suppressalis attractant provided by the invention can be accurately applied to the detection and prediction of the Chilo suppressalis situation, a large amount of trapping and mating interference, and has important significance for comprehensively treating the Chilo suppressalis.
Description
Technical Field
The invention belongs to the technical field of biotechnology, and particularly relates to a composition for simultaneously attracting female and male moths of chilo suppressalis and application thereof.
Background
Chilo Suppressalis (Chilo Suppressalis) is an important lepidoptera pest on rice in China, and can cause a large amount of yield reduction of the rice. In recent years, the occurrence range of chilo suppressalis in China is gradually expanded, outbreaks are more and more frequent, and loss is more serious. Besides rice, Chilo suppressalis also damage crops such as corn, sugarcane, cane shoots, sorghum, wheat and the like.
The main control means for chilo suppressalis in the past is chemical pesticide. However, chemical pesticides bring about a series of negative environmental effects: causing environmental pollution of atmosphere, water source and the like; killing non-target organisms to cause damage to a farmland ecosystem; the pesticide resistance of the chilo suppressalis is very high after long-term use of the pesticide; bringing potential risks to the health of people.
In order to avoid adverse consequences caused by pesticides, sustainable agricultural development is realized, and pollution-free prevention and control of chilo suppressalis are urgently developed. Insect sex pheromones are a novel green pest prevention and control technology developed in recent years. Because of its advantages of high efficiency, strong specificity, no drug resistance, no toxicity and no residue, people are more and more attentive and certain progress has been made.
The conventional striped rice borer sex attractant mainly attracts a male adult, but striped rice borers have a mating habit for many times, and the prevention and control effect of attracting part of male moths is limited. The host odor substance can simultaneously attract the male and female adult chilo suppressalis, but the trapping efficiency is low, and the application to production is difficult. Therefore, the synergistic composition of the two types of attracting substances is screened and searched by combining the advantages of the two types of attracting substances to obtain the compound attractant, the attracting capability of male moths is enhanced, and part of female moths are attracted at the same time, so that better green prevention and control effects are achieved.
Disclosure of Invention
In order to solve the problem that the sex attractant of the chilo suppressalis can only trap and kill male worms and is difficult to achieve effective control, the invention designs and prepares the composition for trapping the female moths of the chilo suppressalis, the male moths are significantly more than the sex attractant by the formula, and the male moths can be simultaneously trapped by the female moths.
Based on the purpose, the chilo suppressalis attractant provided by the invention mainly comprises two parts: chilo suppressalis sex attractant and host odor composition.
The sex attractant is provided by Beijing Zhongjie Siamese Co., Ltd, and is used for attracting the core of the rubber head.
The host odor composition comprises the following components in parts by weight:
1-5% of limonene
Trans-2-hexenal 10-20%
10 to 20 percent of caryophyllene
45 to 79 percent of shogaol
And the sum of the weight percentages of the four components is 100 percent.
Optionally, the host-odor composition consists of the following components in respective weight percentages:
2-4% of limonene
12 to 18 percent of shogaol
12 to 18 percent of caryophyllene
Trans-2-hexenal 60-74%
And the sum of the weight percentages of the two components is 100 percent.
Preferably, the host odor composition consists of the following components in percentage by weight:
3-4% of limonene
15 to 17 percent of shogaol
15 to 17 percent of caryophyllene
Trans-2-hexenal 62-67%
And the sum of the weight percentages of the two components is 100 percent.
Preferably, the host-odor composition consists of the following components in respective weight percentages:
3.5 percent of limonene
Shogaol 16%
Caryophyllene 16%
Trans-2-hexenal 64.5%
The invention also provides an application of the Chilo suppressalis attractant, which comprises the steps of uniformly mixing the Chilo suppressalis host odor composition with the organic solvent difficult to volatilize, loading the mixture into polyethylene tube disorientation filaments with sealed two ends, binding and fixing the disorientation filaments and the sex attractant on a sticky trap, slowly releasing the Chilo suppressalis attractant, trapping and killing the Chilo suppressalis female and male moths, and reducing the offspring harm of the Chilo suppressalis attractant.
Optionally, the non-volatile organic solvent is selected from at least one of liquid paraffin, mineral oil, and sunflower oil.
Optionally, 10-70 mg of the host odor composition and 0.1-0.5 mL of the nonvolatile solvent are placed in each piece of the disorientation silk.
Preferably, 30-50 mg of the host odor composition and 0.1-0.5 mL of the nonvolatile solvent are placed in each piece of the disorientation silk.
The invention also provides a method for controlling and/or monitoring chilo suppressalis, which comprises the step of applying the chilo suppressalis attractant to a habitat of the chilo suppressalis in cooperation with the corresponding trap.
The Chilo suppressalis attractant provided by the invention can be applied to Chilo suppressalis emotion prediction and mass trapping and killing, obviously reduces progeny harm and has important significance for comprehensive treatment. The chilo suppressalis attractant has strong specificity, does not generate resistance, has no harm to natural enemies, is nontoxic to human and livestock, and is environment-friendly; the stress resistance is strong, and the wind, the rain and the sunshine are not afraid; simple preparation, simple use, long lasting period and feasible conditions. The attractant composition is used for monitoring the insect situation, the accuracy is high, and the insect trapping amount can accurately predict the occurrence dynamics of chilo suppressalis; the pesticide can also be used for trapping and killing a large number of chilo suppressalis in the field, and can obviously reduce the number of chilo suppressalis in the field and reduce the population density of progeny insects.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to specific embodiments below.
In recent years, with the enhancement of environmental awareness and the requirement of agricultural sustainability development, the research on the prevention and treatment technology of chilo suppressalis enters a new period. The attractant is used for trapping and killing a large amount of attractant to prevent and control chilo suppressalis, so that the population quantity of adult insects is directly reduced, and the offspring harm is reduced, thereby achieving the aim of effectively controlling the chilo suppressalis.
Researches prove that the host odor substance has a guiding effect on egg laying of female moths, and meanwhile, experiments find that the odor substance and striped rice borer sex pheromone have a remarkable synergistic effect, so that the effect of the sex attractant for trapping male moths can be remarkably improved on one hand, and female moths can be attracted on the other hand. A formula of the Chilo suppressalis attractant composition is obtained through screening and optimizing a large number of field tests.
Example 1
As an embodiment of the invention, the chilo suppressalis attractant is composed of a chilo suppressalis rubber head sex attractant and a host odor composition. The total amount of the host odor composition is 40mg, and the host odor composition comprises the following components in parts by weight: 4 parts of limonene, 12 parts of zingiberene, 12 parts of caryophyllene and 72 parts of trans-2-hexenal.
Example 2
As an embodiment of the invention, the chilo suppressalis attractant is composed of a chilo suppressalis rubber head sex attractant and a host odor composition. The total amount of the host odor composition is 40mg, and the host odor composition comprises the following components in parts by weight: 3 parts of limonene, 15 parts of zingiberene, 15 parts of caryophyllene and 67 parts of trans-2-hexenal.
Example 3
As an embodiment of the invention, the chilo suppressalis attractant is composed of a chilo suppressalis rubber head sex attractant and a host odor composition. The total amount of the host odor composition is 40mg, and the host odor composition comprises the following components in parts by weight: 2 parts of limonene, 18 parts of zingiberene, 18 parts of caryophyllene and 62 parts of trans-2-hexenal.
Example 4
As an embodiment of the invention, the chilo suppressalis attractant is composed of a chilo suppressalis rubber head sex attractant and a host odor composition. The total amount of the host odor composition is 40mg, and the host odor composition comprises the following components in parts by weight: 3 parts of limonene, 16 parts of zingiberene, 16 parts of caryophyllene and 65 parts of trans-2-hexenal.
Example 5
As an embodiment of the invention, the chilo suppressalis attractant is composed of a chilo suppressalis rubber head sex attractant and a host odor composition. The total amount of the host odor composition is 40mg, and the host odor composition comprises the following components in parts by weight: 3.5 parts of limonene, 15 parts of zingiberene, 16 parts of caryophyllene and 65.5 parts of trans-2-hexenal.
Example 6
As an embodiment of the invention, the chilo suppressalis attractant is composed of a chilo suppressalis rubber head sex attractant and a host odor composition. The total amount of the host odor composition is 40mg, and the host odor composition comprises the following components in parts by weight: 3.5 parts of limonene, 16 parts of zingiberene, 16 parts of caryophyllene and 64.5 parts of trans-2-hexenal.
Example 7
As an embodiment of the invention, the chilo suppressalis attractant is composed of a chilo suppressalis rubber head sex attractant and a host odor composition. The total amount of the host odor composition is 30mg, and the host odor composition comprises the following components in parts by weight: 3.5 parts of limonene, 16 parts of zingiberene, 16 parts of caryophyllene and 64.5 parts of trans-2-hexenal.
Example 8
As an embodiment of the invention, the chilo suppressalis attractant is composed of a chilo suppressalis rubber head sex attractant and a host odor composition. The total amount of the host odor composition is 50mg, and the host odor composition comprises the following components in parts by weight: 3.5 parts of limonene, 16 parts of zingiberene, 16 parts of caryophyllene and 64.5 parts of trans-2-hexenal.
The invention also provides application of the Chilo suppressalis attractant in prevention, control and/or monitoring of the Chilo suppressalis, and the application form of the Chilo suppressalis attractant provided by the invention can be various. Specifically, the application can comprise loading the Chilo suppressalis host odor composition into polyethylene tube disorientation filaments with sealed two ends, and fixing the Chilo suppressalis host odor composition and a sex attractant core at the center of a sticky trap simultaneously, so that the Chilo suppressalis attractant is slowly released for trapping Chilo suppressalis adults.
In embodiments, the non-volatile organic solvent is selected from at least one of liquid paraffin, mineral oil, sunflower oil. Preferably, the less volatile organic solvent is liquid paraffin.
In the embodiment, the disorientation silk is made of polyethylene material, and can also be made of polypropylene plastic and high-density polyethylene material. The wall thickness of the labyrinth wire tube wall is 0.01-0.1 mm.
In the embodiment, 10-70 mg of Chilo suppressalis attractant and 0.1-5 mL of nonvolatile solvent are placed in each piece of disorientation silk.
As a preferred embodiment of the invention, 30-50 mg of Chilo suppressalis attractant and 0.3mL of nonvolatile solvent are put into each 1mL of plastic bottle.
To further illustrate the feasibility of the Chilo suppressalis attractant provided by the present invention, specific tests are described below.
Experiments on the trapping effect of the Chilo suppressalis attractant are carried out in Yuchuan town of Wujing city, Hubei province from 7 month 8 to 8 month 7 in 2013.
And uniformly mixing limonene, zingiberene, caryophyllene and trans-2-hexenal according to the respective weight part ratio of the embodiments 1-8 to respectively obtain the chilo suppressalis host odor composition. The host odor compositions of Chilo suppressalis in examples 1-8 are respectively dissolved in liquid paraffin, then loaded in the disorientation silk, and then the disorientation silk and the sex attractant are simultaneously fixed at 400cm2The pest sticking plate trap is used for trapping chilo suppressalis adults. In the experiment, 30mg of Chilo suppressalis attractant and 0.3mL of liquid paraffin are placed in each piece of isotropic silk.
Selecting a continuous rice field with the area of more than 10 mu and consistent rice variety, growth and fertilizer and water management conditions as a test field, uniformly and randomly arranging 10 insect sticking plate traps containing the attractant and simultaneously arranging a light trap. And (4) surveying the number of the chilo suppressalis imagoes trapped every 7 days from the third day after the setting, simultaneously replacing the sticky trap, replacing the trapping positions, and continuously surveying for one month.
The test effect is as follows:
TABLE 1
Wherein, the Chilo suppressalis attractant of the comparative example 1 is a rubber head lure provided by Beijing Zhongjie Site technology Limited.
The test result shows that: the total trapping amount and the female and male trapping amounts of the Chilo suppressalis attractant provided by the invention are obviously higher than the trapping amounts of lamplight and comparative examples. The attractant provided by the invention is used for predicting, forecasting and trapping the emergence period of chilo suppressalis, so that the accuracy of prediction can be improved, and the trapping activity is high.
Therefore, the chilo suppressalis attractant provided by the invention can be applied to the detection and prediction of the chilo suppressalis situation and a large amount of trapping and killing, and has important significance for comprehensively treating the chilo suppressalis. The chilo suppressalis attractant has strong specificity, does not generate resistance, has no harm to natural enemies, is nontoxic to human and livestock, and is environment-friendly; the stress resistance is strong, and the wind, the rain and the sunshine are not afraid; simple preparation, simple use, long lasting period and feasible conditions. The sex attractant is used for monitoring insect conditions, has high accuracy, and can accurately predict the occurrence dynamics of chilo suppressalis by trapping insects; the pesticide can also be used for trapping and killing a large number of chilo suppressalis in the field, and can obviously reduce the number of chilo suppressalis in the field and reduce the population density of progeny insects.
Those of ordinary skill in the art will understand that: the invention is not to be considered as limited to the specific embodiments thereof, but is to be understood as being modified in all respects, all changes and equivalents that come within the spirit and scope of the invention.
Claims (5)
1. The chilo suppressalis attractant is characterized by being composed of a chilo suppressalis sex attractant and a host odor composition;
the sex attractant directly purchases commercial products;
the host odor composition comprises the following components in parts by weight: 2-4 parts of limonene, 12-18 parts of zingiberene, 12-18 parts of caryophyllene and 60-74 parts of trans-2-hexenal.
2. The application of the Chilo suppressalis attractant as claimed in claim 1, wherein the Chilo suppressalis host odor composition and the non-volatile solvent are uniformly mixed, then the mixed solution is placed in a sealed labyrinth wire, and then the labyrinth wire and the sex attractant are simultaneously fixed on a sticky trap, so that the Chilo suppressalis attractant is slowly released for trapping Chilo suppressalis adults.
3. The use of a Chilo suppressalis attractant as claimed in claim 2, wherein the non-volatile solvent is selected from at least one of liquid paraffin, mineral oil, and sunflower oil.
4. The use of a Chilo suppressalis attractant as claimed in claim 2, wherein the disorientation silk is polyethylene disorientation silk.
5. The application of the Chilo suppressalis attractant as claimed in claim 2, wherein 10-70 mg of the Chilo suppressalis attractant and 0.1-0.5 mL of a nonvolatile solvent are placed into the disorientation silk.
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| CN201510057949.XA CN104782594B (en) | 2015-02-03 | 2015-02-03 | Compound attractant for simultaneously attracting female moths and male moths of chilo suppressalis and application thereof |
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| CN201510057949.XA CN104782594B (en) | 2015-02-03 | 2015-02-03 | Compound attractant for simultaneously attracting female moths and male moths of chilo suppressalis and application thereof |
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| CN107455392B (en) * | 2017-09-05 | 2020-02-04 | 浙江省农业科学院 | Active composition based on camphor and α -pinene, chilo suppressalis attractant and application |
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| CN115067331B (en) * | 2022-06-10 | 2023-09-01 | 深圳百乐宝生物农业科技有限公司 | Chilo suppressalis attractant composition and application thereof |
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| CN103070170A (en) * | 2013-01-28 | 2013-05-01 | 扬州大学 | Composite attractant for attracting rice leaf roller female and male moths and application of composite attractant |
| CN103245647A (en) * | 2013-04-27 | 2013-08-14 | 中国计量学院 | Citrus fruit fly odorant binding protein-based attractant screening method |
| CN103782965A (en) * | 2014-02-08 | 2014-05-14 | 云南农业大学 | Method for testing influence of corn volatile matter on oviposition behavior of Asiatic corn borer |
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2015
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| US3743747A (en) * | 1971-06-14 | 1973-07-03 | Mobil Oil Corp | Oxo-derivatives of limonene and dipentene used as fungicides |
| CN101341934A (en) * | 2007-07-13 | 2009-01-14 | 东莞市盛唐化工有限公司 | Citrus fruit fly food attractant |
| CN101861094A (en) * | 2007-09-25 | 2010-10-13 | 关键基因股份有限公司 | Plant volatiles |
| CN103070170A (en) * | 2013-01-28 | 2013-05-01 | 扬州大学 | Composite attractant for attracting rice leaf roller female and male moths and application of composite attractant |
| CN103245647A (en) * | 2013-04-27 | 2013-08-14 | 中国计量学院 | Citrus fruit fly odorant binding protein-based attractant screening method |
| CN103782965A (en) * | 2014-02-08 | 2014-05-14 | 云南农业大学 | Method for testing influence of corn volatile matter on oviposition behavior of Asiatic corn borer |
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