CN104758326B - A kind of preparation method of grey dish water extract applied to resisting allergic rhinitis medicine - Google Patents
A kind of preparation method of grey dish water extract applied to resisting allergic rhinitis medicine Download PDFInfo
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- CN104758326B CN104758326B CN201510165636.6A CN201510165636A CN104758326B CN 104758326 B CN104758326 B CN 104758326B CN 201510165636 A CN201510165636 A CN 201510165636A CN 104758326 B CN104758326 B CN 104758326B
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- 239000000284 extract Substances 0.000 title claims abstract description 69
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 51
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 206010039085 Rhinitis allergic Diseases 0.000 title claims description 12
- 201000010105 allergic rhinitis Diseases 0.000 title claims description 12
- 239000003814 drug Substances 0.000 title claims description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 24
- 239000011780 sodium chloride Substances 0.000 claims abstract description 12
- 239000000706 filtrate Substances 0.000 claims abstract description 7
- 238000012869 ethanol precipitation Methods 0.000 claims abstract description 4
- 241000737404 Chenopodium opulifolium Species 0.000 claims abstract description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 14
- 239000013049 sediment Substances 0.000 claims description 8
- 238000000605 extraction Methods 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 4
- 235000006760 Acer pensylvanicum Nutrition 0.000 claims description 3
- 241000219312 Chenopodium Species 0.000 claims description 3
- 238000000034 method Methods 0.000 claims description 3
- 102000039446 nucleic acids Human genes 0.000 abstract description 8
- 108020004707 nucleic acids Proteins 0.000 abstract description 8
- 150000007523 nucleic acids Chemical class 0.000 abstract description 8
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 abstract description 5
- 239000011575 calcium Substances 0.000 abstract description 5
- 229910052791 calcium Inorganic materials 0.000 abstract description 5
- 108090000623 proteins and genes Proteins 0.000 abstract description 5
- 102000004169 proteins and genes Human genes 0.000 abstract description 5
- 150000004676 glycans Chemical class 0.000 abstract description 4
- 229920001282 polysaccharide Polymers 0.000 abstract description 4
- 239000005017 polysaccharide Substances 0.000 abstract description 4
- -1 wherein Chemical class 0.000 abstract description 3
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- 230000000172 allergic effect Effects 0.000 abstract description 2
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- 230000000774 hypoallergenic effect Effects 0.000 abstract description 2
- 239000004480 active ingredient Substances 0.000 abstract 1
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- 238000000926 separation method Methods 0.000 abstract 1
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- 235000018102 proteins Nutrition 0.000 description 4
- 241000700199 Cavia porcellus Species 0.000 description 3
- 206010020751 Hypersensitivity Diseases 0.000 description 3
- 208000030961 allergic reaction Diseases 0.000 description 3
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- 238000012360 testing method Methods 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- NTYJJOPFIAHURM-UHFFFAOYSA-N Histamine Chemical compound NCCC1=CN=CN1 NTYJJOPFIAHURM-UHFFFAOYSA-N 0.000 description 2
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- RJGDLRCDCYRQOQ-UHFFFAOYSA-N anthrone Chemical compound C1=CC=C2C(=O)C3=CC=CC=C3CC2=C1 RJGDLRCDCYRQOQ-UHFFFAOYSA-N 0.000 description 2
- 230000003266 anti-allergic effect Effects 0.000 description 2
- 229960005070 ascorbic acid Drugs 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- KZJWDPNRJALLNS-VPUBHVLGSA-N (-)-beta-Sitosterol Natural products O[C@@H]1CC=2[C@@](C)([C@@H]3[C@H]([C@H]4[C@@](C)([C@H]([C@H](CC[C@@H](C(C)C)CC)C)CC4)CC3)CC=2)CC1 KZJWDPNRJALLNS-VPUBHVLGSA-N 0.000 description 1
- CSVWWLUMXNHWSU-UHFFFAOYSA-N (22E)-(24xi)-24-ethyl-5alpha-cholest-22-en-3beta-ol Natural products C1CC2CC(O)CCC2(C)C2C1C1CCC(C(C)C=CC(CC)C(C)C)C1(C)CC2 CSVWWLUMXNHWSU-UHFFFAOYSA-N 0.000 description 1
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 1
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 1
- KLEXDBGYSOIREE-UHFFFAOYSA-N 24xi-n-propylcholesterol Natural products C1C=C2CC(O)CCC2(C)C2C1C1CCC(C(C)CCC(CCC)C(C)C)C1(C)CC2 KLEXDBGYSOIREE-UHFFFAOYSA-N 0.000 description 1
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 1
- 206010006956 Calcium deficiency Diseases 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000871189 Chenopodiaceae Species 0.000 description 1
- LPZCCMIISIBREI-MTFRKTCUSA-N Citrostadienol Natural products CC=C(CC[C@@H](C)[C@H]1CC[C@H]2C3=CC[C@H]4[C@H](C)[C@@H](O)CC[C@]4(C)[C@H]3CC[C@]12C)C(C)C LPZCCMIISIBREI-MTFRKTCUSA-N 0.000 description 1
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 1
- ARVGMISWLZPBCH-UHFFFAOYSA-N Dehydro-beta-sitosterol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)CCC(CC)C(C)C)CCC33)C)C3=CC=C21 ARVGMISWLZPBCH-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 1
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 1
- 239000005642 Oleic acid Substances 0.000 description 1
- 108010058846 Ovalbumin Proteins 0.000 description 1
- 235000021314 Palmitic acid Nutrition 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 244000292697 Polygonum aviculare Species 0.000 description 1
- 235000006386 Polygonum aviculare Nutrition 0.000 description 1
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 1
- 206010070834 Sensitisation Diseases 0.000 description 1
- 229930003451 Vitamin B1 Natural products 0.000 description 1
- 229930003471 Vitamin B2 Natural products 0.000 description 1
- 239000000370 acceptor Substances 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- MJVXAPPOFPTTCA-UHFFFAOYSA-N beta-Sistosterol Natural products CCC(CCC(C)C1CCC2C3CC=C4C(C)C(O)CCC4(C)C3CCC12C)C(C)C MJVXAPPOFPTTCA-UHFFFAOYSA-N 0.000 description 1
- LGJMUZUPVCAVPU-UHFFFAOYSA-N beta-Sitostanol Natural products C1CC2CC(O)CCC2(C)C2C1C1CCC(C(C)CCC(CC)C(C)C)C1(C)CC2 LGJMUZUPVCAVPU-UHFFFAOYSA-N 0.000 description 1
- NJKOMDUNNDKEAI-UHFFFAOYSA-N beta-sitosterol Natural products CCC(CCC(C)C1CCC2(C)C3CC=C4CC(O)CCC4C3CCC12C)C(C)C NJKOMDUNNDKEAI-UHFFFAOYSA-N 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000003610 charcoal Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000002242 deionisation method Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000003979 eosinophil Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 229960001340 histamine Drugs 0.000 description 1
- 210000003630 histaminocyte Anatomy 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 1
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 1
- 235000020778 linoleic acid Nutrition 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- WQEPLUUGTLDZJY-UHFFFAOYSA-N n-Pentadecanoic acid Natural products CCCCCCCCCCCCCCC(O)=O WQEPLUUGTLDZJY-UHFFFAOYSA-N 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 1
- 235000021313 oleic acid Nutrition 0.000 description 1
- 229940092253 ovalbumin Drugs 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 206010039083 rhinitis Diseases 0.000 description 1
- 229960002477 riboflavin Drugs 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- KZJWDPNRJALLNS-VJSFXXLFSA-N sitosterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CC[C@@H](CC)C(C)C)[C@@]1(C)CC2 KZJWDPNRJALLNS-VJSFXXLFSA-N 0.000 description 1
- 229950005143 sitosterol Drugs 0.000 description 1
- NLQLSVXGSXCXFE-UHFFFAOYSA-N sitosterol Natural products CC=C(/CCC(C)C1CC2C3=CCC4C(C)C(O)CCC4(C)C3CCC2(C)C1)C(C)C NLQLSVXGSXCXFE-UHFFFAOYSA-N 0.000 description 1
- 235000015500 sitosterol Nutrition 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 235000010374 vitamin B1 Nutrition 0.000 description 1
- 239000011691 vitamin B1 Substances 0.000 description 1
- 235000019164 vitamin B2 Nutrition 0.000 description 1
- 239000011716 vitamin B2 Substances 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Include the invention discloses a kind of grey dish water extract and its application, the step of the preparation method of the grey dish water extract of the ash:Grey goosefoot leaf piece is taken, pulp is crushed, is extracted with NaCl extract solutions, then filtered, ethanol precipitation is added in filtrate, precipitation and separation simultaneously decolourizes, and obtains the grey dish water extract;Compared with prior art, grey dish water extract of the present invention includes the Multiple components such as polysaccharide, protein, nucleic acid, wherein, polysaccharide component can be effectively combined the calcium to dissociate in human body, promote calcium absorption;Meanwhile the active ingredient in the grey dish water extract of the ash can also be combined optionally with the IgE in human body, there is pre- hypo-allergenic effect to the people of allergic constitution.
Description
Technical field
The present invention relates to a kind of extract of grey dish, more particularly to a kind of grey dish water extract and its answer
With.
Background technology
Grey dish, You Mingli, the wild grey dish of alias, grey knotweed head grass etc., are Chenopodiaceae annual herb plant.Mainly it is born in field
Between, rand, roadside, Around the house etc..Grey dish seedling and young stem and leaf edible, delicious flavour, mouthfeel is tender, nutritious.
Grey dish young stem and leaf contains protein, fat, carbohydrate, crude fibre, calcium, phosphorus, iron, carrotene, vitamin B1, vitamin B2, dimension life
Plain PP, vitamin c, also contain volatile oil, such as palmitic acid, oleic acid, linoleic acid and sitosterol;Particularly wherein profuse Hu
Radish element and vitamin c help to strengthen immune function of human body.Anaemia can be prevented by eating grey dish, promote upgrowth and development of children,
Also there are certain health care functions to person in middle and old age calcium deficiency person.
The content of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of applied to resisting allergic rhinitis medicine
The preparation method of grey dish water extract.
The present invention is achieved by the following technical solutions:
The invention provides a kind of preparation method of the grey dish water extract applied to resisting allergic rhinitis medicine, the system
Preparation Method comprises the following steps:
(1) grey goosefoot leaf piece is taken, crushes pulp, is 7.5~8.0 with pH value at 70~80 DEG C, mass concentration 3
~4% NaCl extract solutions are extracted, and obtain first extract;
(2) first extract of step (1) is filtered, obtains filtrate, ethanol precipitation is added in filtrate, centrifuged, precipitated
Thing;
(3) sediment of step (2) is decolourized, obtains the grey dish water extract.
Preferably, in the step (1), the pH value of NaCl extract solutions is 8.0.
Preferably, in the step (1), the mass ratio of the NaCl extract solutions and pulpous state ash goosefoot leaf piece is 8:1.
Preferably, in the step (1), the speed of agitator of extraction is 300rpm, and the time of extraction is 30min.
Preferably, in the step (2), the volume ratio of the ethanol and first extract is 4:1.
Preferably, it is in the step (3), the step of decolouring:Per 1g sediments with 15ml water dissolve, and adjust pH value to
6~6.5, then at 70~80 DEG C, activated carbon decolorizing is added, is finally filtered off except activated carbon.
Preferably, the time of activated carbon decolorizing is 30min.
The present invention has advantages below compared with prior art:The invention provides one kind to be applied to resisting allergic rhinitis medicine
Grey dish water extract preparation method, the grey dish water extract of the ash includes the Multiple components such as polysaccharide, protein, nucleic acid, wherein,
Polysaccharide component can be effectively combined the calcium to dissociate in human body, promote calcium absorption;Meanwhile in the grey dish water extract of the ash it is effective into
Divide optionally to be combined with the IgE in human body, have pre- hypo-allergenic effect to the people of allergic constitution.
Brief description of the drawings
Fig. 1 is the yield curve figure of the grey dish water extract that NaCl extract solutions extract under different pH value.
Embodiment
Embodiments of the invention are elaborated below, the present embodiment is carried out lower premised on technical solution of the present invention
Implement, give detailed embodiment and specific operating process, but protection scope of the present invention is not limited to following implementation
Example.
Embodiment 1
1st, the preparation of grey dish water extract:
(1) stage casing of grey dish fresh leaf blade is taken, pulp is crushed, is divided into 7 groups, at 70~80 DEG C, is separately added into pH
The NaCl extract solutions that the mass concentration being worth for 6.0,6.5,7.0,7.5,8.0,8.5,9.0 is 4% are extracted, and are obtained and are just carried
Liquid, wherein, the mass ratio of NaCl extract solutions and pulpous state ash goosefoot leaf piece in every group is 8:1, the speed of agitator of extraction is
300rpm, the time of extraction is 30min;
(2) first extract of step (1) is filtered, obtains filtrate, the ethanol precipitation of 4 times of volumes is added in filtrate, centrifuged,
Obtain sediment;
(3) by the sediment deionized water dissolving of step (2), wherein, it is water-soluble with 15ml deionization per 1g sediments
Solution, and pH value is adjusted to 6~6.5, then at 70~80 DEG C, activated carbon decolorizing 30min is added, is finally filtered off except activity
Charcoal, freeze, obtain the dried frozen aquatic products of grey dish water extract;
(4) yield of 7 groups of grey dish water extracts is calculated, obtains result as shown in Figure 1:
Quality × 100% of grey dish fresh leaf blade before quality/extraction of yield (%)=grey dish water extract dried frozen aquatic products
It can be seen that in Fig. 1, when the pH value of NaCl extract solutions is 8.0, the yield highest of grey dish water extract, most preferably to carry
Take pH value.
It is test material to test the grey dish water extract for taking pH value to be 8.0 groups of acquisitions below.
2nd, the Components identification of grey dish water extract
The sugar content of grey dish water extract, by weight, the sugar content of the grey dish water extract of the ash are determined using anthrone reagent
For 67%;
Utilize the protein content of the grey dish water extract of BCA determination of protein concentration kit measurement, by weight, ash ash
The protein content of dish water extract is 24%;
The nucleic acid content of grey dish water extract, by weight, the grey dish water of the ash are determined using ROCHE kit for detecting nucleic acid
The nucleic acid content of extract is 4%;
Other materials component content is 5%.
3rd, grey dish water extract antiallergic effect experiment
SERUM IgE rise is the most strong prompting of anaphylactia, and anaphylactogen enters body inducing producing specificity IgE,
IgE is attached to mast cell and eosinophil, body is entered to the special sensitization of the anaphylactogen, when anaphylactogen again
During contact, combined with the IgE acceptors on cell membrane and cause a series of biochemical reactions, then discharge histamine etc. it is various with
Allergic reaction and the biologically active medium of inflammation-related.
It is anaphylactogen from ovalbumin in the present embodiment, by being injected intraperitoneally, atomizer is fumigated and the method for collunarium,
The animal model (IgE kits test positive+) of duplicator's anaphylactoid rhinitis on cavy, obtain anaphylactic guinea pig.
Above-mentioned anaphylactic guinea pig is divided into four groups, every group 20, about 500 grams every, freezed with the grey dish water extract
Product are fed, daily respectively to feed once sooner or later, 0.2 gram every every time, are taken one group of cavy weekly, are detected with IgE kits, meter
The ratio (positive turns negative number/20 × 100%) that positive (+) turns negative (-) is calculated, obtains result as shown in table 1 below:
Table 1:Turn (-) ratio with grey dish water extract feeding anaphylactic guinea pig (+)
As can be seen from Table 1, during feeding grey dish water extract of the present invention, the allergic reaction of cavy
The ratio for switching to feminine gender from the positive gradually rises, and after feeding four weeks, the cure rate of cavy allergic reaction reaches 70%, explanation
Grey dish water extract has preferable antiallergic effect.
Embodiment 2
Present embodiments provide another grey dish water extract, be 7.5 using pH value in its preparation method, mass concentration
Extracted for 3% NaCl extract solutions, other steps are the same as embodiment 1, calculated yield 5.4%.
The sugar content of grey dish water extract, by weight, the sugar content of the grey dish water extract of the ash are determined using anthrone reagent
For 61%.
Utilize the protein content of the grey dish water extract of BCA determination of protein concentration kit measurement, by weight, ash ash
The protein content of dish water extract is 26%.
The nucleic acid content of grey dish water extract, by weight, the grey dish water of the ash are determined using ROCHE kit for detecting nucleic acid
The nucleic acid content of extract is 6%.
Other materials component content is 7%.
Claims (7)
- A kind of 1. preparation method of grey dish water extract applied to resisting allergic rhinitis medicine, it is characterised in that the preparation side Method comprises the following steps:(1) grey goosefoot leaf piece is taken, crushes pulp, is 7.5~8.0 with pH value at 70~80 DEG C, mass concentration is 3~4% NaCl extract solutions extracted, obtain first extract;(2) first extract of step (1) is filtered, obtains filtrate, ethanol precipitation is added in filtrate, centrifuged, obtain sediment;(3) sediment of step (2) is decolourized, obtains the grey dish water extract.
- 2. a kind of preparation method of grey dish water extract applied to resisting allergic rhinitis medicine according to claim 1, Characterized in that, in the step (1), the pH value of NaCl extract solutions is 8.0.
- 3. a kind of preparation method of grey dish water extract applied to resisting allergic rhinitis medicine according to claim 1, Characterized in that, in the step (1), the mass ratio of the NaCl extract solutions and pulpous state ash goosefoot leaf piece is 8:1.
- 4. a kind of preparation method of grey dish water extract applied to resisting allergic rhinitis medicine according to claim 1, Characterized in that, in the step (1), the speed of agitator of extraction is 300rpm, and the time of extraction is 30min.
- 5. a kind of preparation method of grey dish water extract applied to resisting allergic rhinitis medicine according to claim 1, Characterized in that, in the step (2), the volume ratio of the ethanol and first extract is 4:1.
- 6. a kind of preparation method of grey dish water extract applied to resisting allergic rhinitis medicine according to claim 1, It is characterized in that, in the step (3), the step of decolouring:Dissolved per 1g sediments with 15ml water, and adjust pH value to 6~ 6.5, then at 70~80 DEG C, activated carbon decolorizing is added, is finally filtered off except activated carbon.
- 7. a kind of preparation method of grey dish water extract applied to resisting allergic rhinitis medicine according to claim 1, Characterized in that, in the step (3), the time of activated carbon decolorizing is 30min.
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