CN1318436C - Method for extracting general saponin from chenopodium album - Google Patents
Method for extracting general saponin from chenopodium album Download PDFInfo
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- CN1318436C CN1318436C CNB2005100428354A CN200510042835A CN1318436C CN 1318436 C CN1318436 C CN 1318436C CN B2005100428354 A CNB2005100428354 A CN B2005100428354A CN 200510042835 A CN200510042835 A CN 200510042835A CN 1318436 C CN1318436 C CN 1318436C
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- oak
- leaved goosefeet
- solvent
- total saponins
- ethanol
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- Detergent Compositions (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention discloses a method for extracting a total saponin from a plant of chenopodium album and the application of the extracted total saponin for natural washing agents. The preparation method comprises the following steps: coarse powder of the plant of the chenopodium album is extracted by a solvent for three times; extracting solutions are combined and filtered; the solvent is recovered, and the extracting solution is concentrated. The concentrated solution is put on LSA-5B type macroporous adsorptive resin and eluted by water, 30% alcohol and 70% alcohol in gradient; the eluents of 70% alcohol are mixed; the solvent recovered until the solvent is dry; the total saponin is obtained. The method is simple and practical; the chenopodium plants are weeds along roads and are easy to obtain. The content of the total saponin is high than 1.7%. The present invention provides plentiful raw materials and a convenient extraction method for preparing natural washing agents.
Description
Technical field
The present invention relates to the extraction and separation method of the natural detergent of detergent industry, particularly a kind of method of from oak-leaved goosefeet, extracting total saponins.
Background technology
Chenopodiaceae Chenopodium plant oak-leaved goosefeet Chenopodium album, popular name ash dish, little Leaf ash can be edible, the secretion that increases saliva is arranged and help digest and hemopoietic function, and between the weeds that are grown in field, roadside, the Rural areas distributed pole is wide in China more.At present, the research emphasis of oak-leaved goosefeet is its chemical ingredients and pharmaceutical use,, still from oak-leaved goosefeet, extracts total saponins and its relevant report as natural detergent according to the data-searching that the applicant did.Its reference is as follows:
[1] yellow snow peak, Li Fan, Chen Cailiang. the Mexican Tea Herb The Chemical Constituents. Chinese natural drug .2003,1 (1): 24-26.
[2] Yang Xianwen etc. the Study on Preparation of Radix Platycodi total saponins. Shenyang Pharmaceutical University's journal.2002，19(6)：440-442。
[3]Lawaud C，Vonurquenne L，Bal P，Pouny I.Saponins fromChenopodium album.Fitorerapia.2000，71(3)：338-340。
[4] chief editor such as Xu Lianying. the pharmaceutical technology (tcd) technology is resolved. People's Health Publisher .2003 first version in December .84-87.
[5] Chen Zaixing, Yuan Changlu. macroporous adsorbent resin and the application in the field of Chinese medicines thereof. the journal .2004 of Traditional Chinese Medicinal College of Liaoning, 6 (2): 124.
[6]Dellagrea M，Previtera L，Zarrelli A. A new xyloside fromChenopodium album.Natural product research.2005，19(1)：87-90。
Summary of the invention
The objective of the invention is to, a kind of method of extracting total saponins from oak-leaved goosefeet is provided, the total saponins that this method is extracted can be used as natural detergent and uses.
To achieve these goals, the technical solution that the present invention takes is that a kind of method of extracting total saponins from oak-leaved goosefeet is characterized in that, carries out according to the following steps:
1) extracts
Get the oak-leaved goosefeet meal of certainweight, use 8 times, 6 times, 6 times of the plant meal to measure the industrial methanol refluxing extraction three times respectively, extraction time is respectively 1h, 40min, 30min, merge alcohol extract, use paper pulp filtering, concentrate to such an extent that plant meal weight and methanol solvate volume ratio are 1: 3 oak-leaved goosefeet concentrated solution.
2) separate
With the oak-leaved goosefeet concentrated solution with LSA-5B type macroporous adsorbent resin on the speed of 4mL/min, with resin volume ratio and plant meal weight ratio absorption in 6: 1 oak-leaved goosefeet concentrated solution, after treating absorption fully, use distilled water, 30% ethanol, 70% ethanol velocity slope wash-out successively with 4mL/min, merge 70%7 alcohol eluens, be recycled to driedly, promptly obtain total saponins.
Experiment showed, the total saponins that adopts method preparation of the present invention, have the function of washing composition, can be used as natural washing composition and use.
Description of drawings
Fig. 1 is that wash-out becomes thin-layered figure.
The present invention is described in further detail below in conjunction with embodiment that the contriver provides.
Embodiment
1. extract:
Get the meal 10g of this oak-leaved goosefeet, use the industrial alcohol refluxing extraction three times of 80mL, 60mL, 60mL respectively, extraction time is respectively 1h, 40min, 30min, merges alcohol extract, use paper pulp filtering, filtrate is reclaimed solvent to 30mL (the plant meal is 1: 3 with the liquor capacity ratio).
2. separate:
With concentrated solution with 60mLLSA-5B type macroporous adsorbent resin on the speed of 4mL/min, treat absorption fully after, with distilled water, 30% ethanol, 70% ethanol gradient elution, the speed of wash-out is 4mL/min.Merge each solvent elution part, reclaim solvent.
3. differentiate
Each wash-out is differentiated partly concrete working method is as follows:
(1) foam experiment: get the 2mL70% ethanol eluate, use the 2mL water dissolution behind the evaporate to dryness, filter, filtrate is changed in the test tube, violent jolting, if produce a large amount of foams, foam volume is greater than 1/3 of liquor capacity, the time length, then showing had saponin(e more than 10min.This reaction of 70% ethanol elution part is positive.
(2) aceticanhydride-strong sulfuric acid response: get the 2mL70% ethanol eluate, evaporate to dryness, residue drip the dissolving of 1mL Glacial acetic acid, add the 1mL aceticanhydride again, drip a vitriol oil in the edge of solution then, if contain saponin(e, then color promptly becomes pink, change into dirty green immediately.This reaction of 70% ethanol elution part is positive.
(3) thin-layer chromatography: each solvent elution part point sample on the silica gel G plate, is made developping agent with chloroform-methanol (9.5: 0.5), launch the back and take out, dry, spray 10% ethanol solution of sulfuric acid, 105 ℃ of heating 5min colour developing.If contain saponin component, then manifest the red-purple spot.Thin layer figure sees Fig. 1.
Go up experiment according to this and show, contain saponin component in 70% ethanol eluate.After i.e. 70% ethanol eluate recovery solvent is extremely done, then obtain total saponins.
4. cleaning function checking
Adopt emulsion process that the total saponins that separation obtains is carried out the checking of cleaning function, the aqueous solution of getting 4mL water, 25 times liquid detergent of dilution and total saponins adds 10 vegetables oil, jolting 1min in three test tubes, leave standstill lower floor's clarity after record separation time and the layering.Experimental result sees Table 1.
Table 1 emulsification experimental result
| The examination number | Determinand | Separation time (s) | Lower floor's situation after the layering |
| 1 2 3 | Water liquid detergent total saponins | 32 90 118 | Clarification is muddy |
Test the result factually and show that the saponin component in the Chenopodium plant has certain cleaning function.
Claims (2)
1. a method of extracting total saponins from oak-leaved goosefeet is characterized in that, carries out according to the following steps:
1) extracts
Get the oak-leaved goosefeet meal of certainweight, use 8 times, 6 times, 6 times of the plant meal to measure the industrial methanol refluxing extraction three times respectively, extraction time is respectively 1h, 40min, 30min, merge alcohol extract, use paper pulp filtering, concentrate to such an extent that plant meal weight and methanol solvate volume ratio are 1: 3 oak-leaved goosefeet concentrated solution;
2) separate
With the oak-leaved goosefeet concentrated solution with LSA-5B type macroporous adsorbent resin on the speed of 4mL/min, with resin solution volume ratio and plant meal weight ratio absorption in 6: 1 oak-leaved goosefeet concentrated solution, after treating absorption fully, use distilled water, 30% ethanol, 70% ethanol velocity slope wash-out successively with 4mL/min, merge 70% ethanol eluate, be recycled to driedly, promptly obtain total saponins.
2. the total saponins that adopts the method for claim 1 to extract gained from oak-leaved goosefeet is used for the application of natural detergent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100428354A CN1318436C (en) | 2005-06-20 | 2005-06-20 | Method for extracting general saponin from chenopodium album |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100428354A CN1318436C (en) | 2005-06-20 | 2005-06-20 | Method for extracting general saponin from chenopodium album |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1733783A CN1733783A (en) | 2006-02-15 |
| CN1318436C true CN1318436C (en) | 2007-05-30 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB2005100428354A Expired - Fee Related CN1318436C (en) | 2005-06-20 | 2005-06-20 | Method for extracting general saponin from chenopodium album |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104758326B (en) * | 2015-04-09 | 2018-02-23 | 安徽农业大学 | A kind of preparation method of grey dish water extract applied to resisting allergic rhinitis medicine |
| CN104983756B (en) * | 2015-05-22 | 2018-10-02 | 昆明理工大学 | The new application of chenopodium album linn whole plants extract |
| CN113862092A (en) * | 2021-07-30 | 2021-12-31 | 神木和德生态农业有限公司 | Detergent composition, detergent and preparation method |
-
2005
- 2005-06-20 CN CNB2005100428354A patent/CN1318436C/en not_active Expired - Fee Related
Non-Patent Citations (4)
| Title |
|---|
| 江苏药学与临床研究 刘欣等，19-20，藜的化学成分研究 2002 * |
| 江苏药学与临床研究 刘欣等，19-20，藜的化学成分研究 2002;辽宁中医学院学报 陈再兴等，124-125，大孔吸附树脂及其在中药领域中的应用 2004;生物技术 赵良忠等，66-67，灰灰菜抗菌物质提取及抗菌效果研究 2004 * |
| 生物技术 赵良忠等，66-67，灰灰菜抗菌物质提取及抗菌效果研究 2004 * |
| 辽宁中医学院学报 陈再兴等，124-125，大孔吸附树脂及其在中药领域中的应用 2004 * |
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| Publication number | Publication date |
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| CN1733783A (en) | 2006-02-15 |
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Granted publication date: 20070530 Termination date: 20100620 |