CN104757328A - Microalgae compound living body bait used for raising fishes and production method thereof - Google Patents
Microalgae compound living body bait used for raising fishes and production method thereof Download PDFInfo
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- CN104757328A CN104757328A CN201510187502.4A CN201510187502A CN104757328A CN 104757328 A CN104757328 A CN 104757328A CN 201510187502 A CN201510187502 A CN 201510187502A CN 104757328 A CN104757328 A CN 104757328A
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Abstract
The invention discloses a microalgae compound living body bait used for raising fishes and a production method thereof. The microalgae compound living body bait is formed by microalgae algae liquid obtained by cultivating following microalgae seeds and mixing according to a cell quantity ratio: 10%-15% of chaetoceros muelleri, 15%-20% of phaeodactylum tricornutum bohlin, 10%-15% of isochrysis galbana parke, 10%-15% of haematococcus pluvialis, 30%-35% of chlorella pyrenoidosa and 15%-20% of pavlova viridis; and the total density is 1.2*10<5>-5.8*10<5> cells/millimeter. The production method comprises the following steps: (1) cultivating the microalgae seeds by using a photobioreactor to obtain the microalgae algae liquid with the cultivation density of 1.1*10<9>-5.6*10<10> cells/millimeter; and (2) preparing the microalgae compound living body bait from the microalgae algae liquid according to the cell quantity ratio to obtain the microalgae compound living body bait with the total density of 1.2*10<5>-5.8*10<5> cells/millimeter. According to the microalgae compound living body bait, the plurality of types of microalgae cells are prepared into the microalgae compound living body bait and the nutrition is balanced; and the survival rate of raising the fishes can be improved and the morbidity can be reduced.
Description
Technical field
The invention belongs to technical field of microalga biology, particularly a kind of micro-algae compound living body bait for fish hatching and production method thereof.
Background technology
The nutrition of micro-algae is abundant and comprehensive, is the primary food of aquiculture animal seed.Micro-algae protein quality is higher, especially the content of essential amino acid and fish meal quite even more excellent, as aspartic acid and glutamic acid can up to 7.1-12.9%, the content of cysteine, methionine, tryptophan and histidine is between 0.4-3.2%, and other amino acid contents are between 3.2-13.5%.Polyunsaturated fatty acid (PUFA) is the necessary nutrition of aquatic livestock paedomorphosis, and micro-algae is the originally producer of PUFA.In many micro-algaes, especially diatom and chrysophyceae, containing abundant eicosapentaenoic acid (EPA), DHA (DHA) and arachidonic acid (ARA).Also containing abundant vitamin in micro-algae, as V
a, V
c, V
d, V
e, V
k, V
brace (B1, B2, B3, B5, B6, B12), biotin, beta carotene and folic acid etc., A wide selection of colours and designs and rich content are suitable with fish feed recommendation with the content in groups of people's based food.Also containing micro-algae sterol that various structures is special in micro-algae, have been found that and confirm have kind more than 10, as being rich in fucosterol in Chaetoceros, campesterol and 24-methylene cholesterol is rich in Skeletonema Greville, hailian seaweed and flat algae, brassicasterol is rich in, containing stigmasterol and cupreol in Ba Fuzao in red born of the same parents algae and Isochrysis galbana.Sterol is the important component part of animal cell membrane, can play and regulate animal body internal hormone level, suppress cholesterol absorption, promote the important physiological roles such as cholesterol alienation.Enrich and the nutritional labeling of equilibrium (albumen, aliphatic acid, carbohydrate) and various bioactivator (PUFA, vitamin, sterol) in micro-algae, the nutritional need that aquiculture animal is grown at the normal growth of Seedling Stage can be met.
For aquaculture industry demand, external existing corresponding product comes out, have micro-algae concentrate and micro-algae dry powder dissimilar, with single algae kind or many algaes kind composite, make the product such as high protein type, high PUFA type, be applicable to prawn, shellfish hatchery respectively, and wheel animalcule, halogen worm nutrient enrichment.Major brand has the Alga Mac series of products of Aqua Fauna company, the series of products such as Roti Grow, Roti Green, InstantAlgae of Reed Mariculture company, relate to the algae kinds such as chlorella, micro-plan ball algae, Isochrysis galbana, Ba Fuzao, hailian seaweed and flat algae.
Domestic most aquatic fry growing enterprise, is all provide both culturing microalgae facility for oneself, produces all kinds of bait voluntarily and use micro-algae in nursery is produced.But general nursery all generally lacks corresponding professional technique strength, respective algae pond and the extensive cultivation of natural water body can only be utilized, bait micro-algae kind matter, production technology and methods for using them all done things in his own way, to cause that microalge is chaotic, supply is unstable, nutritional labeling is uneven, bait is tired low, lack multi items intensive manufacture application technology; Meanwhile, be limited to the restriction of micro-algae high-density breeding, harvesting technique and concentrate preservation technology, domestic almost unified, specialized bait micro-algae quality standard and centralizedly supply point.Due to supply not in time, normal and nursery progress disconnects, and to be difficult to meet in nursery the supply of fresh food and the demand of multi items, causes that survival rate of seedling is low, seedling resistance against diseases is poor, seedling cost is high thus, and it is very unfavorable to form for the later stage.
China's aquaculture scale the first in the world, huge to the demand of bait micro-algae.By the calculating of French scholar Muller-Feuga, every 1,000,000 tail fishs, bivalve and the micro algae biomass required for prawn seed-rearing conversion dry weight is respectively 60,14 and 0.65 kilograms.According to said method, throw seedling amount for foundation with China's these three kinds of aquiculture animals in 2010, just need micro-algae 16000 tons of dry weights, and the output of the micro-algae of China's commercialization is still less than 10000 tons at present, the output of the micro-algae of commercialization aquatic feed is very little.Therefore, the prospect in this market is boundless, economic benefit and social benefit highly significant.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of technique micro-algae compound living body bait for fish hatching simple, time saving and energy saving, with low cost and production method thereof.
For solving the problems of the technologies described above, the technical solution used in the present invention is as follows:
A kind of micro-algae compound living body bait for fish hatching, the micro-algae algae liquid mixed in number of cells ratio after cultivating by following micro-algae algae kind forms: Chaetoceros muelleri 10% ~ 15%, Phaeodactylum tricornutum 15% ~ 20%, Isochrysis galbana 10% ~ 15%, haematococcus pluvialis 10% ~ 15%, chlorella pyrenoidosa 30% ~ 35% and Ba Fu algae 15% ~ 20%, and gross density is 1.2 × 10
5~ 5.8 × 10
5individual cell/mL.
For solving the problems of the technologies described above, another technical solution used in the present invention is as follows:
For a production method for micro-algae compound living body bait of fish hatching, comprise the steps:
(1) algae kind is selected
According to the nutritional need of fish seedling, micro-algae algae kind is selected to be Chaetoceros muelleri, Phaeodactylum tricornutum, Isochrysis galbana, haematococcus pluvialis, chlorella pyrenoidosa and Ba Fuzao;
(2) microdisk electrode
Utilize bioreactor to cultivate respectively the micro-algae algae kind selected in step (1), obtaining density is 1.1 × 10
9~ 5.6 × 10
10micro-algae algae liquid of individual cell/mL;
(3) preparation of micro-algae compound living body bait
By the micro-algae algae liquid obtained in step (2), being mixed with gross density by following number of cells percentage is 1.2 × 10
5-5.8 × 10
5micro-algae compound living body bait of individual cell/mL;
Chaetoceros muelleri 10 ~ 15%, Phaeodactylum tricornutum 15 ~ 20%, Isochrysis galbana 10 ~ 15%, haematococcus pluvialis 10 ~ 15%, chlorella pyrenoidosa 30 ~ 35% and Ba Fu algae 15 ~ 20%;
(4) the micro-algae compound living body bait obtained in step (3) is pressed 0.1 ~ 0.3L/m
3the density of throwing something and feeding of breeding water body is thrown something and fed fish seedling.
Fish seedling described in above-mentioned steps (1) is filefish prelarva.
Cultivation described in above-mentioned steps (2), the cultivation temperature of wherein said Chaetoceros muelleri, Phaeodactylum tricornutum, Isochrysis galbana, haematococcus pluvialis and Ba Fuzao is 23 DEG C, and inoculum density is 1 × 10
4individual cell/mL, culture medium is respectively f/2, f/2, f/2, BBM and Kang Wei side's culture medium; The cultivation temperature of described chlorella pyrenoidosa is 28 DEG C, and inoculum density is 1 × 10
4individual cell/mL, culture medium is BG11 culture medium.
Of the present inventionly for micro-algae compound living body bait of fish hatching and the advantage of production method thereof and beneficial effect be:
(1) microalgae cell is made the nutritive loss that living body bait not only can be avoided because drying causes, and the survival rate of fish seedling can be improved, reduce its incidence of disease.
(2) the micro-algae compound living body bait be made up of multiple microalgae cell can the nutrition that causes because of single micro-algae of quasi-complement unbalanced, improve growth rate and the quality of fish seedling.
(3) micro-algae compound living body bait that the present invention develops has the features such as cost is low, purity is high, effective, and is easy to realize industrial amplification production.
Detailed description of the invention
According to following embodiment, the present invention may be better understood.But those skilled in the art will readily understand, the content described by embodiment only for illustration of the present invention, and should can not limit the present invention described in detail in claims yet.
Embodiment:
For a production method for micro-algae compound living body bait of fish hatching, comprise the steps:
(1) according to the nutritional need of filefish prelarva, Chaetoceros muelleri, Phaeodactylum tricornutum, Isochrysis galbana, haematococcus pluvialis, chlorella pyrenoidosa and Ba Fuzao is selected to be micro-algae algae kind.
(2) utilize bioreactor (ZL201220434882.9) to cultivate respectively the micro-algae algae kind selected in step (1), obtain micro-algae algae liquid.Wherein the cultivation temperature of Chaetoceros muelleri, Phaeodactylum tricornutum, Isochrysis galbana, haematococcus pluvialis and Ba Fuzao is 23 DEG C, and inoculum density is 1 × 10
4individual cell/mL, culture medium is respectively f/2, f/2, f/2, BBM and Kang Wei side's culture medium; Chlorella pyrenoidosa cultivation temperature is 28 DEG C, and inoculum density is 1 × 10
4individual cell/mL, culture medium is BG11 culture medium.After bioreactor is cultivated, obtaining density is respectively 1.1 × 10
9(Chaetoceros muelleri), 3.7 × 10
10(Phaeodactylum tricornutum), 1.5 × 10
10(Isochrysis galbana), 7.8 × 10
9(haematococcus pluvialis), 5.6 × 10
10(chlorella pyrenoidosa), 9.2 × 10
9(Ba Fuzao) micro-algae algae liquid of individual cell/mL.
(3) the micro-algae algae liquid will obtained in step (2), by microalgae cell number proportional arrangement become to be used for filefish prelarva, micro-algae compound living body bait of different gross density.
In embodiment 1-12, microalgae cell number ratio design parameter is as shown in table 1.For evaluating the beneficial effect of micro-algae compound living body bait, with the survival rate of filefish prelarva (%) and the incidence of disease (%) for index, the effect evaluating micro-algae compound living body bait is as shown in table 1.
Table 1
Comparative example 1:
(1) Phaeodactylum tricornutum is selected to be micro-algae algae kind.
(2) utilize bioreactor (ZL201220434882.9) to cultivate the micro-algae algae kind selected in step (1), obtain micro-algae algae liquid.The cultivation temperature of Phaeodactylum tricornutum is 23 DEG C, and inoculum density is 1 × 10
4individual cell/mL, culture medium is f/2 culture medium.After bioreactor is cultivated, obtaining culture density is 3.5 × 10
10micro-algae algae liquid of individual cell/mL.
(3) the micro-algae algae liquid will obtained in step (2), be mixed with for filefish prelarva, gross density is 3.5 × 10
5the microalgae living body bait of individual cell/mL.
(4) the microalgae living body bait obtained in step (3) is pressed 0.3L/m
3water body is thrown something and fed filefish prelarva.
(5) through evaluating, the filefish prelarva 30d utilizing this product to feed, survival rate is 84.9%, and the incidence of disease is 23.3%.
Comparative example 2:
(1) chlorella pyrenoidosa is selected to be micro-algae algae kind.
(2) utilize bioreactor (ZL201220434882.9) to cultivate the micro-algae algae kind selected in step (1), obtain micro-algae algae liquid.The cultivation temperature of chlorella pyrenoidosa is 28 DEG C, and inoculum density is 1 × 10
4individual cell/mL, culture medium is BG11 culture medium.Cultivate through bioreactor, obtaining culture density is 5.2 × 10
10micro-algae algae liquid of individual cell/mL.
(3) the micro-algae algae liquid will obtained in step (2), be mixed with for filefish prelarva, gross density is 5.2 × 10
5the microalgae living body bait of individual cell/mL.
(4) the microalgae living body bait obtained in step (3) is pressed 0.2L/m
3water body is thrown something and fed filefish prelarva.
(5) through evaluating, the filefish prelarva 30d utilizing this product to feed, survival rate is 81.4%, and the incidence of disease is 25.9%.
As fully visible, the micro-algae compound living body bait be made up of multiple microalgae cell adopting the present invention to produce does living body bait than adopting separately a kind of microalgae cell, more can improve the survival rate of fish seedling, and reduce its incidence of disease.And the mixed proportion between different microalgae cell has considerable influence to evaluation indexes such as fish survival rate and the incidences of disease, this nutriment be mainly rich in due to different microalgae cell has difference, as Diatomeae be rich in aliphatic acid, chlorella is rich in protein etc., and the nutriment required for the Different growth phases of fish seedling also can be different, therefore applicable micro-algae compound living body bait need be selected for different fish seedling.
Claims (4)
1. the micro-algae compound living body bait for fish hatching, it is characterized in that, the micro-algae algae liquid mixed in number of cells ratio after cultivating by following micro-algae algae kind forms: Chaetoceros muelleri 10% ~ 15%, Phaeodactylum tricornutum 15% ~ 20%, Isochrysis galbana 10% ~ 15%, haematococcus pluvialis 10% ~ 15%, chlorella pyrenoidosa 30% ~ 35% and Ba Fu algae 15% ~ 20%, and gross density is 1.2 × 10
5~ 5.8 × 10
5individual cell/mL.
2., as claimed in claim 1 for a production method for micro-algae compound living body bait of fish hatching, it is characterized in that, comprise the steps:
(1) algae kind is selected
According to the nutritional need of fish seedling, micro-algae algae kind is selected to be Chaetoceros muelleri, Phaeodactylum tricornutum, Isochrysis galbana, haematococcus pluvialis, chlorella pyrenoidosa and Ba Fuzao;
(2) microdisk electrode
Utilize bioreactor to cultivate respectively the micro-algae algae kind selected in step (1), obtaining density is 1.1 × 10
9~ 5.6 × 10
10micro-algae algae liquid of individual cell/mL;
(3) preparation of micro-algae compound living body bait
By the micro-algae algae liquid obtained in step (2), being mixed with gross density by following number of cells percentage is 1.2 × 10
5~ 5.8 × 10
5micro-algae compound living body bait of individual cell/mL;
Chaetoceros muelleri 10 ~ 15%, Phaeodactylum tricornutum 15 ~ 20%, Isochrysis galbana 10 ~ 15%, haematococcus pluvialis 10 ~ 15%, chlorella pyrenoidosa 30 ~ 35% and Ba Fu algae 15 ~ 20%;
(4) the micro-algae compound living body bait obtained in step (3) is pressed 0.1 ~ 0.3L/m
3the density of throwing something and feeding of breeding water body is thrown something and fed fish seedling.
3. the production method of the micro-algae compound living body bait for fish hatching according to claim 2, it is characterized in that, the fish seedling described in step (1) is filefish prelarva.
4. the production method of the micro-algae compound living body bait for fish hatching according to claim 2, it is characterized in that, cultivation described in step (2), the cultivation temperature of wherein said Chaetoceros muelleri, Phaeodactylum tricornutum, Isochrysis galbana, haematococcus pluvialis and Ba Fuzao is 23 DEG C, and inoculum density is 1 × 10
4individual cell/mL, culture medium is respectively f/2, f/2, f/2, BBM and Kang Wei side's culture medium; The cultivation temperature of described chlorella pyrenoidosa is 28 DEG C, and inoculum density is 1 × 10
4individual cell/mL, culture medium is BG11 culture medium.
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Cited By (6)
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| CN105053689A (en) * | 2015-08-21 | 2015-11-18 | 临沂大学 | Puffing feed using marine microalgae as main raw materials, for takifugu obscurus |
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| CN109090380A (en) * | 2018-09-12 | 2018-12-28 | 浙江海洋大学 | A kind of bait formula for axe clam floating larvae phase fortification |
| CN111183930A (en) * | 2020-02-25 | 2020-05-22 | 海南晨海水产有限公司 | Algae cultivation and water fertilization method for outdoor ecological breeding of grouper |
| CN113812543A (en) * | 2020-06-19 | 2021-12-21 | 国投生物科技投资有限公司 | Algae powder type seedling raising bait, application thereof and method for raising seedlings of prawns in daphnia-shaped period |
| CN113812360A (en) * | 2020-06-19 | 2021-12-21 | 国投生物科技投资有限公司 | Algae powder type seedling raising bait, application thereof and shellfish artificial seedling raising method |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105053689A (en) * | 2015-08-21 | 2015-11-18 | 临沂大学 | Puffing feed using marine microalgae as main raw materials, for takifugu obscurus |
| CN105053689B (en) * | 2015-08-21 | 2019-01-01 | 临沂大学 | It is a kind of using marine microalgae as the fugu obscurus expanded pellet diet of primary raw material |
| CN108125018A (en) * | 2018-02-01 | 2018-06-08 | 秦烽曦 | A kind of Animal nutrition material and its application |
| CN109090380A (en) * | 2018-09-12 | 2018-12-28 | 浙江海洋大学 | A kind of bait formula for axe clam floating larvae phase fortification |
| CN111183930A (en) * | 2020-02-25 | 2020-05-22 | 海南晨海水产有限公司 | Algae cultivation and water fertilization method for outdoor ecological breeding of grouper |
| CN113812543A (en) * | 2020-06-19 | 2021-12-21 | 国投生物科技投资有限公司 | Algae powder type seedling raising bait, application thereof and method for raising seedlings of prawns in daphnia-shaped period |
| CN113812360A (en) * | 2020-06-19 | 2021-12-21 | 国投生物科技投资有限公司 | Algae powder type seedling raising bait, application thereof and shellfish artificial seedling raising method |
| CN113812360B (en) * | 2020-06-19 | 2023-01-06 | 国投生物科技投资有限公司 | Algae powder type seedling raising bait, application thereof and shellfish artificial seedling raising method |
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Application publication date: 20150708 Assignee: Center for technology transfer Jiangsu University of Science and Technology Assignor: JIANGSU University OF SCIENCE AND TECHNOLOGY Contract record no.: X2021980006173 Denomination of invention: The invention relates to a microalgae composite living bait for fish seedling raising and a production method thereof Granted publication date: 20180424 License type: Common License Record date: 20210714 |
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