CN104799082B - A kind of compound living body bait of microalgae for shellfish hatchery and its production method - Google Patents
A kind of compound living body bait of microalgae for shellfish hatchery and its production method Download PDFInfo
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Abstract
The invention discloses a kind of compound living body bait of microalgae for shellfish hatchery and its production method, the microalgae algae solution that the compound living body bait of microalgae is mixed by following microalgae algae kind after culture in number of cells ratio forms：Chaetoceros muelleri 15% 20%, cocolith 20% 25%, Isochrysis galbana 5% 10%, haematococcus pluvialis 15% 20%, chlorella pyrenoidosa 25% 30% and P latymonas subcordifomis 10% 15%, and gross density is 1 × 105‑5×105Individual cell/mL.Production method is as follows：(1) microalgae algae kind is cultivated respectively using bioreactor, it is 1 × 10 to obtain culture density9‑5×1010Individual cell/mL microalgae algae solution；(2) microalgae algae solution is configured to the compound living body bait of microalgae in number of cells ratio, it is 1 × 10 to obtain gross density5‑5×105Individual cell/mL compound the living body bait of microalgae.Compound living body bait is made in a variety of microalgae cells by the application, and nutrition is balanced, can effectively improve the survival rate of shellfish seedling, and reduce its incidence of disease.
Description
Technical field
The invention belongs to technical field of microalga biology, the more particularly to a kind of compound living body bait of microalgae for shellfish hatchery
And its production method.
Background technology
The aquaculture of the economic shellfishes such as oyster, mussel, clam and scallop share shared in world's fish production is increasingly
Greatly, already as one of pillar in living marine resources industry.Optimal bait of the microalgae as shellfish seedling, is shellfish hatchery
Unique suitable food source.
In recent years, with various countries' culture fishery process of industrialization fast development, Mirco algal food production technology development
Rapidly, applicating and exploitation of the microalgae in aquaculture feed is developed rapidly, and Mirco algal food is shared in aquatic feeds
Ratio is also increasing.During shellfish seed and breeding production, the effect of microalgae mainly has three types：(1) by special
Fresh aquatic food bait of the microalgae cell of different processing concentration or High Density Cultivation directly as seedling such as shellfishes；(2) with microalgae original powder
Form is researched and developed as high-grade feed additive according to the composition of the nutritional need of different breeding kind, different microalgae nutritional ingredient
Special Mirco algal food；(3) Principles of Ecosystem of " zooplankter of microalgae one " food chain is utilized, utilizes Different Nutrition type
The protozoans such as microdisk electrode wheel animalcule, artemia, and then develop high-grade indirect bait.The method for evaluating Mirco algal food effect, mainly
It is directly to carry out microalgae as bait to feed experiment, compares the survival rate and the incidence of disease of shellfish；Microalgae of analyzing and researching it is biochemical into
The content and proportion of composing divided, are compareed with the nutritional need of shellfish；Or that two methods can combine into comparison is comprehensive
The bait effect for various microalgaes of objectively analyzing and researching.
At present, the Aguaro Mirco algal foods of U.S.'s exploitation are prepared using the ratio of different microalgae, can meet different breeding product
The demand of kind；The Cell System companies of Britain develop the microalgae feed rich in high unsaturated fatty acids such as EPA or DHA；Day
Originally a variety of concentration Mirco algal foods are developed, can need to be produced in batches according to the different of fish, shrimp, crab, shellfish etc..But due to micro-
Growth characteristics specific to algae cause the industrialization degree of its large-scale production still very low, up to the present, only open
The design and its application of culture systems are relatively more successful, have been amplified to 180,000 m of TaiWan, China2(production chlorella) and Mexico
City produces 200,000 m of spirulina2Or even big arrive western australia biotech company 50 hectares.But open system is universal
There is growth parameter(s) to be difficult to control, and culture environment is unstable；It is easily contaminated, sterile culture is difficult to realize, yield is more low
Shortcoming, is only used for spirulina, and the minority such as chlorella and salt algae is resistant to the microdisk electrode of extreme environment growth；To Chaetoceros,
The large-scale culture of the economic algaes such as chrysophyceae is difficult to be applicable, make Mirco algal food product exploitation and commercialization by very big
Limitation.
The content of the invention
The technical problems to be solved by the invention are to provide that a kind of technique is simple, time saving and energy saving, cost is cheap is used for shellfish
The compound living body bait of microalgae and its production method of class nursery.
In order to solve the above technical problems, the technical solution adopted by the present invention is as follows：
The compound living body bait of microalgae for shellfish hatchery, be by following microalgae algae kind after culture in number of cells ratio
The microalgae algae solution composition of mixing：Chaetoceros muelleri (15%-20%), cocolith (20%-25%), Isochrysis galbana (5%-
10%), haematococcus pluvialis (15%-20%), chlorella pyrenoidosa (25%-30%) and P latymonas subcordifomis (10%-15%), and
Gross density is 1 × 105-5×105Individual cell/mL.
A kind of production method of the compound living body bait of microalgae for shellfish hatchery, comprises the following steps：
(1) algae kind selects：
According to the nutritional need of shellfish seedling, selection microalgae algae kind is Chaetoceros muelleri, cocolith, Isochrysis galbana, rain
Raw haematococcus, chlorella pyrenoidosa and P latymonas subcordifomis；Described shellfish seedling is Pacific oyster seedling (eyebot larvae phase)；
(2) microdisk electrode：
The algae kind of selection in step (1) is cultivated respectively using bioreactor (ZL201220434882.9),
Obtain the microalgae algae solution that density is 1 × 109-5 × 1010 cell/mL；
(3) preparation of the compound living body bait of microalgae：
The microalgae algae solution that will be obtained in step (2), it is configured in number of cells ratio for shellfish seedling, total close
Spend the compound living body bait of microalgae for 1 × 105-5 × 105 cell/mL.The compound living body bait formula of microalgae is as follows：
(4) the compound living body bait of microalgae obtained in step (3) is fed into shellfish seedling, it is 0.1-0.3L/ to feed density
m3Breeding water body.
The beneficial effects of the invention are as follows：
(1) nutritive loss caused by drying can not only be avoided by microalgae cell being made into living body bait, and can be carried
The survival rate of high shellfish seedling, reduces its incidence of disease.
(2) made of a variety of microalgae cells the compound living body bait of microalgae can nutrition of the quasi-complement caused by single microalgae not
Equilibrium, improve the growth rate and quality of shellfish seedling.
(3) the compound living body bait of microalgae that the present invention is developed has the characteristics that cost is low, purity is high, effect is good, and easily
In realizing industrial amplification production.
Embodiment
According to following embodiments, the present invention may be better understood.It is however, as it will be easily appreciated by one skilled in the art that real
Apply the content described by example and be merely to illustrate the present invention, without should be also without limitation on sheet described in detail in claims
Invention.
Embodiment：
(1) according to the nutritional need of Pacific oyster seedling (eyebot larvae phase), Chaetoceros muelleri, cocolith, ball are selected
Isochrysis galbana, haematococcus pluvialis, chlorella pyrenoidosa and P latymonas subcordifomis are microalgae algae kind.
(2) the microalgae algae kind of selection in step (1) is trained using bioreactor (ZL201220434882.9)
Support, obtain microalgae algae solution.The wherein culture of Chaetoceros muelleri, cocolith, Isochrysis galbana, haematococcus pluvialis and P latymonas subcordifomis
Temperature is 23 DEG C, and inoculum density is 1 × 104Individual cell/mL, culture medium are respectively f/2, NMB3, f/2, BBM and the culture of Kang Wei side
Base；Chlorella pyrenoidosa cultivation temperature is 28 DEG C, and inoculum density is 1 × 104Individual cell/mL, culture medium are BG11 culture mediums.Through
After bioreactor culture, it is 1.0 × 10 to obtain density respectively9(Chaetoceros muelleri), 2.7 × 1010(cocolith), 1.5 ×
1010(Isochrysis galbana), 6.8 × 109(haematococcus pluvialis), 5.0 × 1010(chlorella pyrenoidosa), 9.2 × 109It is (sub- heart-shaped flat
Algae) individual cell/mL microalgae algae solution.
(3) the microalgae algae solution that will be obtained in step (2), by microalgae cell number proportional arrangement into for Pacific oyster children
The compound living body bait of microalgae of seedling, different gross densities.
Microalgae cell number ratio design parameter is as shown in table 1 in embodiment 1-12.To evaluate the compound living body bait of microalgae
Beneficial effect, with the survival rate (%) and the incidence of disease (%) of Pacific oyster seedling for index, evaluate the compound live body bait of microalgae
The effect of material is as shown in table 2.
Table 1
Table 2
Comparative example 1：
(1) it is microalgae algae kind to select Chaetoceros muelleri.
(2) the microalgae algae kind of selection in step (1) is trained using bioreactor (ZL201220434882.9)
Support, obtain microalgae algae solution.The cultivation temperature of Chaetoceros muelleri is 23 DEG C, and inoculum density is 1 × 104Individual cell/mL, culture medium are
F/2 culture mediums.Through bioreactor culture, it is 1.5 × 10 to obtain culture density9Individual cell/mL microalgae algae solution.
(3) the microalgae algae solution that will be obtained in step (2), be configured to for Pacific oyster seedling, gross density for 1.5 ×
105Individual cell/mL microalgae living body bait.
(4) the microalgae living body bait obtained in step (3) is pressed into 0.3L/m3Water body feeds Pacific oyster seedling.
(5) through evaluation, the Pacific oyster seedling 30d fed using this product, survival rate 85.5%, the incidence of disease is
23.6%.
Comparative example 2：
(1) it is microalgae algae kind to select chlorella pyrenoidosa.
(2) the microalgae algae kind of selection in step (1) is trained using bioreactor (ZL201220434882.9)
Support, obtain microalgae algae solution.The cultivation temperature of chlorella pyrenoidosa is 28 DEG C, and inoculum density is 1 × 104Individual cell/mL, culture medium
For BG11 culture mediums.Through bioreactor culture, it is 4.2 × 10 to obtain culture density10Individual cell/mL microalgae algae solution.
(3) the microalgae algae solution that will be obtained in step (2), be configured to for Pacific oyster seedling, gross density for 4.2 ×
105Individual cell/mL microalgae living body bait.
(4) the microalgae living body bait obtained in step (3) is pressed into 0.2L/m3Water body feeds Pacific oyster seedling.
(5) through evaluation, the Pacific oyster seedling 30d fed using this product, survival rate 80.3%, the incidence of disease is
26.1%.
It can be seen that the compound living body bait of the microalgae made of a variety of microalgae cells produced using the application is than individually using one
Kind microalgae cell does living body bait, can more improve the survival rate of shellfish seedling, and reduce its incidence of disease.And different microalgae cell
Between mixed proportion have considerable influence to evaluation indexes such as shellfish survival rate and the incidences of disease, this is thin mainly due to different microalgae
The nutriment that born of the same parents are rich in has difference, and such as Diatomeae is rich in protein rich in aliphatic acid, chlorella, and in shellfish seedling
Nutriment required for different growth phases would also vary from, therefore need to be directed to the suitable microalgae of different shellfish seedling selection
Compound living body bait.
Claims (3)
1. for shellfish hatchery the compound living body bait of microalgae, it is characterised in that be by following microalgae algae kind after culture by cell
The microalgae algae solution composition of number ratio mixing：Chaetoceros muelleri 15%-20%, cocolith 20%-25%, Isochrysis galbana 5%-
10%th, haematococcus pluvialis 15%-20%, chlorella pyrenoidosa 25%-30% and P latymonas subcordifomis 10%-15%, and gross density
For 1 × 105-5×105Individual cell/mL.
2. the production method of the compound living body bait of the microalgae for shellfish hatchery described in claim 1, it is characterised in that including
Following steps：
(1) algae kind selects：
According to the nutritional need of shellfish seedling, selection microalgae algae kind is Chaetoceros muelleri, the life of cocolith, Isochrysis galbana, rain is red
Ball algae, chlorella pyrenoidosa and P latymonas subcordifomis；
(2) microdisk electrode：
The algae kind of selection in step (1) is cultivated respectively using bioreactor, it is 1 × 10 to obtain density9-5×1010
Individual cell/mL microalgae algae solution；
(3) preparation of the compound living body bait of microalgae：
The microalgae algae solution that will be obtained in step (2), gross density is configured to as 1 × 10 by following number of cells percentage5-5×
105Individual cell/mL compound the living body bait of microalgae；
By the compound living body bait of microalgae obtained in step (3) according to 0.1-0.3L/m3The density that feeds of breeding water body feeds shellfish
Seedling.
3. the production method of the compound living body bait of the microalgae according to claim 2 for shellfish hatchery, it is characterised in that
Described shellfish seedling is Pacific oyster seedling, and growth period is the eyebot larvae phase.
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| CN107637550A (en) * | 2017-09-11 | 2018-01-30 | 浙江海洋大学 | A kind of method that heavy metal in body of shellfish is filtered out using bait micro-algae |
| CN108125018A (en) * | 2018-02-01 | 2018-06-08 | 秦烽曦 | A kind of Animal nutrition material and its application |
| CN111387342A (en) * | 2020-03-26 | 2020-07-10 | 内蒙古锡牧生命科学有限公司 | Animal microalgae nutrient solution and application thereof |
| CN113812543A (en) * | 2020-06-19 | 2021-12-21 | 国投生物科技投资有限公司 | Algae powder type seedling raising bait, application thereof and method for raising seedlings of prawns in daphnia-shaped period |
| CN113812360B (en) * | 2020-06-19 | 2023-01-06 | 国投生物科技投资有限公司 | Algae powder type seedling raising bait, application thereof and shellfish artificial seedling raising method |
| CN114794332A (en) * | 2022-05-09 | 2022-07-29 | 广东南洋海洋产业投资有限公司 | Bait formula for enhancing nutrition of oyster fries in larval stage |
| CN114747519B (en) * | 2022-05-13 | 2023-05-09 | 广东海洋大学 | Seawater pearl Bei Yangshi pearl culturing method |
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| CN1653938A (en) * | 2005-03-09 | 2005-08-17 | 青岛森淼实业有限公司 | Mirco algal food nutrient intensifying agent prescription and its preparing process |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87104387A (en) * | 1986-06-24 | 1988-01-06 | 日清制油株式会社 | Bait for feeding larvae of crustaceans and shellfish |
| CN1653938A (en) * | 2005-03-09 | 2005-08-17 | 青岛森淼实业有限公司 | Mirco algal food nutrient intensifying agent prescription and its preparing process |
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Application publication date: 20150729 Assignee: Center for technology transfer Jiangsu University of Science and Technology Assignor: JIANGSU University OF SCIENCE AND TECHNOLOGY Contract record no.: X2021980006173 Denomination of invention: The invention relates to a microalgae composite living bait for shellfish seedling raising and a production method thereof Granted publication date: 20180306 License type: Common License Record date: 20210714 |
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