CN104738308A - Livestock feed additive for effectively increasing feed utilization rate and preparation process thereof - Google Patents
Livestock feed additive for effectively increasing feed utilization rate and preparation process thereof Download PDFInfo
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- CN104738308A CN104738308A CN201510184402.6A CN201510184402A CN104738308A CN 104738308 A CN104738308 A CN 104738308A CN 201510184402 A CN201510184402 A CN 201510184402A CN 104738308 A CN104738308 A CN 104738308A
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- bacillus subtilis
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- bacillus megaterium
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention provides a livestock feed additive for effectively increasing feed utilization rate and a preparation process thereof, and relates to a livestock feed additive and a preparation process thereof, and the livestock feed additive is used for solving the problem that coarse fiber materials in the feed are difficultly digested by livestock and thus the feed utilization rate is small. The feed additive comprises bacillus megatherium liquid, bacillus subtilis liquid and a carrier. The method comprises the following steps: 1, selecting bacillus megatherium, inoculating to a nutrient broth culture medium to obtain activated bacillus megatherium, and selecting bacillus subtilis and inoculating to the nutrient broth culture medium to obtain activated bacillus subtilis; 2, inoculating the activated bacillus megatherium and activated bacillus subtilis to the nutrient broth culture medium to culture to obtain seed liquid; 3, inoculating the seed liquid into a fermenting tank to culture; and 4, adding the bacillus megatherium, liquid to the carrier, fermenting and drying, then adding the bacillus subtilis liquid, and fermenting and drying to obtain the feed additive. The additive can be used for increasing the livestock feed utilization rate.
Description
Technical field
The present invention relates to a kind of animal feeding-stuff additive and preparation technology thereof.
Background technology
Feed addictive refers at Feed Manufacturing processing, a small amount of or micro substance that adds in use procedure, in feed consumption seldom but effect significantly.Feed addictive is the raw material that modern feed industry must use, and to strengthening basal feed nutritive value, improve breeding performonce fo animals, ensure animal health, save feed cost, improving the aspects such as livestock products quality has obvious effect.
Containing a large amount of crude fibre material in feed, and feed with wind dry form, these crude fibre materials are often difficult to be digested by livestock, and plant recovery of nutrient is low.
Summary of the invention
The present invention is that the crude fibre material that will solve in feed is difficult to be digested by livestock, causes the problem that efficiency of feed utilization is low, provides a kind of feed addictive and preparation technology thereof of effective raising efficiency of feed utilization.
The animal feeding-stuff additive that the present invention effectively improves efficiency of feed utilization comprises bacillus megaterium bacterium liquid, bacillus subtilis bacterium liquid and carrier, the concentration of described bacillus megaterium bacterium liquid is 1,000,000,000/mL, the concentration of described bacillus subtilis bacterium liquid is 1,000,000,000/mL, described carrier by weight percentage by 40% powdered rice hulls, the corn flour of 40%, the dregs of beans of 10% and 10% wheat bran form, the weight of bacillus megaterium bacterium liquid accounts for 40% of total weight of carrier, and the weight of bacillus subtilis bacterium liquid accounts for 40% of total weight of carrier.
Bacillus megaterium is bought from Institute of Microbiology, Heilongjiang Academy of Sciences, and trade name is Bacillus, is numbered 1.223.
Bacillus subtilis (Bacillus Subtilis) is bought from Institute of Microbiology, Heilongjiang Academy of Sciences, is numbered 1.421.
The preparation technology of above-mentioned feed addictive, carries out according to the following steps:
One, picking bacillus megaterium is inoculated on nutrient broth medium, cultivates 7 days at 27 DEG C, and obtain the bacillus megaterium activated, picking bacillus subtilis is inoculated on nutrient broth medium, cultivates 7 days at 27 DEG C, obtains the bacillus subtilis activated;
Two, the bacillus subtilis of the bacillus megaterium of activation and activation is inoculated in nutrient broth medium according to the inoculum concentration of 2% respectively, in 27 DEG C of shaking table shaken cultivation 7 days, shaking speed is 80 ~ 100 turns/min, obtains bacillus megaterium seed liquor and bacillus subtilis seed liquor;
Three, then bacillus megaterium seed liquor is inoculated in the fermentation tank containing 1000L nutrient broth medium, is cultured to bacterium number in bacillus megaterium zymotic fluid and reaches 1,000,000,000/mL; Bacillus subtilis seed liquor is inoculated in the fermentation tank containing 1000L nutrient broth medium, is cultured to bacterium number in fermentation of bacillus subtilis liquid and reaches 1,000,000,000/mL;
Four, take by weight percentage the powdered rice hulls of 40%, the corn flour of 40%, the dregs of beans of 10% and 10% wheat bran mix, obtain carrier, bacillus megaterium fermentation broth agitation is added even, in 27 DEG C of fermentations 5 ~ 7 days, then 70 ~ 90 DEG C of oven dry in carrier, to water content lower than 10%, add fermentation of bacillus subtilis liquid again to stir, in 27 DEG C of fermentations 5 ~ 7 days, then 70 ~ 90 DEG C of oven dry, to water content lower than 13%, namely obtain feed addictive.
Beneficial effect of the present invention:
1, in feed, add the additive of the present invention of 2%, efficiency of feed utilization can be improved and reach 10% ~ 20%;
2, the present invention utilizes powdered rice hulls to make carrier, and not only twice laid, reduces costs, and not containing other pollutants, is conducive to animal growth;
3, add profitable strain in the carrier, various ectoenzyme (protease, amylase, lipase, cellulase etc.) can be produced, improve the degradation capability of feed, improve the utilization rate of feed, play the effect of preventing and curing diseases.
Detailed description of the invention
Technical solution of the present invention is not limited to following cited detailed description of the invention, also comprises any combination between each detailed description of the invention.
Detailed description of the invention one: the animal feeding-stuff additive that present embodiment effectively improves efficiency of feed utilization comprises bacillus megaterium bacterium liquid, bacillus subtilis bacterium liquid and carrier, the concentration of described bacillus megaterium bacterium liquid is 1,000,000,000/mL, the concentration of described bacillus subtilis bacterium liquid is 1,000,000,000/mL, described carrier by weight percentage by 40% powdered rice hulls, the corn flour of 40%, the dregs of beans of 10% and 10% wheat bran form, the weight of bacillus megaterium bacterium liquid accounts for 40% of total weight of carrier, and the weight of bacillus subtilis bacterium liquid accounts for 40% of total weight of carrier.
Detailed description of the invention two: the preparation technology of present embodiment feed addictive, carry out according to the following steps:
One, picking bacillus megaterium is inoculated on nutrient broth medium, cultivates 7 days at 27 DEG C, and obtain the bacillus megaterium activated, picking bacillus subtilis is inoculated on nutrient broth medium, cultivates 7 days at 27 DEG C, obtains the bacillus subtilis activated;
Two, the bacillus subtilis of the bacillus megaterium of activation and activation is inoculated in nutrient broth medium according to the inoculum concentration of 2% respectively, in 27 DEG C of shaking table shaken cultivation 7 days, shaking speed is 80 ~ 100 turns/min, obtains bacillus megaterium seed liquor and bacillus subtilis seed liquor;
Three, then bacillus megaterium seed liquor is inoculated in the fermentation tank containing 1000L nutrient broth medium, is cultured to bacterium number in bacillus megaterium zymotic fluid and reaches 1,000,000,000/mL; Bacillus subtilis seed liquor is inoculated in the fermentation tank containing 1000L nutrient broth medium, is cultured to bacterium number in fermentation of bacillus subtilis liquid and reaches 1,000,000,000/mL;
Four, take by weight percentage the powdered rice hulls of 40%, the corn flour of 40%, the dregs of beans of 10% and 10% wheat bran mix, obtain carrier, bacillus megaterium fermentation broth agitation is added even, in 27 DEG C of fermentations 5 ~ 7 days, then 70 ~ 90 DEG C of oven dry in carrier, to water content lower than 10%, add fermentation of bacillus subtilis liquid again to stir, in 27 DEG C of fermentations 5 ~ 7 days, then 70 ~ 90 DEG C of oven dry, to water content lower than 13%, namely obtain feed addictive.
Detailed description of the invention three: present embodiment and detailed description of the invention two unlike: in step 3, the inoculum concentration of bacillus megaterium seed liquor is 2%.Other is identical with detailed description of the invention two.
Detailed description of the invention four: present embodiment and detailed description of the invention two or three unlike: in step 3, the inoculum concentration of bacillus subtilis seed liquor is 2%.Other is identical with detailed description of the invention two or three.
Detailed description of the invention five: one of present embodiment and detailed description of the invention two to four unlike: in step 4, the weight of bacillus megaterium zymotic fluid accounts for 40% of total weight of carrier.Other is identical with one of detailed description of the invention two to four.
Detailed description of the invention six: one of present embodiment and detailed description of the invention two to five unlike: in step 4, the weight of fermentation of bacillus subtilis liquid accounts for 40% of total weight of carrier.Other is identical with one of detailed description of the invention two to five.
For verifying beneficial effect of the present invention, carry out following experiment:
Embodiment:
The present embodiment effectively improves the preparation technology of the animal feeding-stuff additive of efficiency of feed utilization, carries out according to the following steps:
One, picking bacillus megaterium is inoculated on nutrient broth medium, cultivates 7 days at 27 DEG C, and obtain the bacillus megaterium activated, picking bacillus subtilis is inoculated on nutrient broth medium, cultivates 7 days at 27 DEG C, obtains the bacillus subtilis activated;
Two, the bacillus subtilis of the bacillus megaterium of activation and activation is inoculated in nutrient broth medium according to the inoculum concentration of 2% respectively, in 27 DEG C of shaking table shaken cultivation 7 days, shaking speed is 90 turns/min, obtains bacillus megaterium seed liquor and bacillus subtilis seed liquor;
Three, then bacillus megaterium seed liquor is inoculated in the fermentation tank containing 1000L nutrient broth medium, is cultured to bacterium number in bacillus megaterium zymotic fluid and reaches 1,000,000,000/mL; Bacillus subtilis seed liquor is inoculated in the fermentation tank containing 1000L nutrient broth medium, is cultured to bacterium number in fermentation of bacillus subtilis liquid and reaches 1,000,000,000/mL;
Four, take by weight percentage the powdered rice hulls of 40%, the corn flour of 40%, the dregs of beans of 10% and 10% wheat bran mix, obtain carrier, bacillus megaterium fermentation broth agitation is added even, in 27 DEG C of fermentations 6 days, then 80 DEG C of oven dry in carrier, to water content lower than 10%, add fermentation of bacillus subtilis liquid again to stir, in 27 DEG C of fermentations 6 days, then 80 DEG C of oven dry, to water content lower than 13%, namely obtain feed addictive.
In step 3, the inoculum concentration of bacillus megaterium seed liquor is 2%.In step 3, the inoculum concentration of bacillus subtilis seed liquor is 2%.
In step 4, the weight of bacillus megaterium zymotic fluid accounts for 40% of total weight of carrier.In step 4, the weight of fermentation of bacillus subtilis liquid accounts for 40% of total weight of carrier.
Feed for growing and fattening pigs and beef cattle, the number of often kind of livestock is 20, arranges experimental group and control group, and experimental group adds feed addictive prepared by the present embodiment in feed, adds certain commercially available brand feed addictive in the feed of control group.The using method of the present embodiment feed addictive: add 2%(quality in feed) the present embodiment feed addictive feed.Experimental result is as shown in the table.
The each livestock of table 1 experimental group is fed result
The each livestock of table 2 control group is fed result
From the data in table 1 and table 2, feed addictive of the present invention can significantly improve efficiency of feed utilization, can improve 10% ~ 20%; The present invention utilizes powdered rice hulls to make carrier, not only twice laid, reduces costs, and not containing other pollutants, is conducive to animal growth.
Claims (6)
1. one kind is effectively improved the animal feeding-stuff additive of efficiency of feed utilization, it is characterized in that this feed addictive comprises bacillus megaterium bacterium liquid, bacillus subtilis bacterium liquid and carrier, the concentration of described bacillus megaterium bacterium liquid is 1,000,000,000/mL, the concentration of described bacillus subtilis bacterium liquid is 1,000,000,000/mL, described carrier by weight percentage by 40% powdered rice hulls, the corn flour of 40%, the dregs of beans of 10% and 10% wheat bran form, the weight of bacillus megaterium bacterium liquid accounts for 40% of total weight of carrier, and the weight of bacillus subtilis bacterium liquid accounts for 40% of total weight of carrier.
2. the preparation technology effectively improving the animal feeding-stuff additive of efficiency of feed utilization as claimed in claim 1, is characterized in that this technique is carried out according to the following steps:
One, picking bacillus megaterium is inoculated on nutrient broth medium, cultivates 7 days at 27 DEG C, and obtain the bacillus megaterium activated, picking bacillus subtilis is inoculated on nutrient broth medium, cultivates 7 days at 27 DEG C, obtains the bacillus subtilis activated;
Two, the bacillus subtilis of the bacillus megaterium of activation and activation is inoculated in nutrient broth medium according to the inoculum concentration of 2% respectively, in 27 DEG C of shaking table shaken cultivation 7 days, shaking speed is 80 ~ 100 turns/min, obtains bacillus megaterium seed liquor and bacillus subtilis seed liquor;
Three, then bacillus megaterium seed liquor is inoculated in the fermentation tank containing 1000L nutrient broth medium, is cultured to bacterium number in bacillus megaterium zymotic fluid and reaches 1,000,000,000/mL; Bacillus subtilis seed liquor is inoculated in the fermentation tank containing 1000L nutrient broth medium, is cultured to bacterium number in fermentation of bacillus subtilis liquid and reaches 1,000,000,000/mL;
Four, take by weight percentage the powdered rice hulls of 40%, the corn flour of 40%, the dregs of beans of 10% and 10% wheat bran mix, obtain carrier, bacillus megaterium fermentation broth agitation is added even, in 27 DEG C of fermentations 5 ~ 7 days, then 70 ~ 90 DEG C of oven dry in carrier, to water content lower than 10%, add fermentation of bacillus subtilis liquid again to stir, in 27 DEG C of fermentations 5 ~ 7 days, then 70 ~ 90 DEG C of oven dry, to water content lower than 13%, namely obtain feed addictive.
3. the preparation technology of the animal feeding-stuff additive of effective raising efficiency of feed utilization according to claim 2, is characterized in that the inoculum concentration of bacillus megaterium seed liquor in step 3 is 2%.
4. the preparation technology of the animal feeding-stuff additive of the effective raising efficiency of feed utilization according to Claims 2 or 3, is characterized in that the inoculum concentration of bacillus subtilis seed liquor in step 3 is 2%.
5. the preparation technology of the animal feeding-stuff additive of effective raising efficiency of feed utilization according to claim 4, is characterized in that the weight of bacillus megaterium zymotic fluid in step 4 accounts for 40% of total weight of carrier.
6. the preparation technology of the animal feeding-stuff additive of effective raising efficiency of feed utilization according to claim 5, is characterized in that the weight of fermentation of bacillus subtilis liquid in step 4 accounts for 40% of total weight of carrier.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510184402.6A CN104738308A (en) | 2015-04-20 | 2015-04-20 | Livestock feed additive for effectively increasing feed utilization rate and preparation process thereof |
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| CN201510184402.6A CN104738308A (en) | 2015-04-20 | 2015-04-20 | Livestock feed additive for effectively increasing feed utilization rate and preparation process thereof |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2168909C1 (en) * | 2000-04-14 | 2001-06-20 | Общество с ограниченной ответственностью "БИОТРОФ" | Strain of bacterium lactobacillus plantarum for fodder ensilage |
| CN102409007A (en) * | 2011-07-04 | 2012-04-11 | 天津市育琪生物技术有限公司 | Bacillus microecological preparation and liquid-solid fermentation combining preparation process thereof |
| CN102409015A (en) * | 2011-12-06 | 2012-04-11 | 北京大北农科技集团股份有限公司 | Composite micro-ecological preparation as well as premixed material and application of preparation in feed additive |
| CN102524534A (en) * | 2011-08-03 | 2012-07-04 | 黑龙江省富天力生物科技有限公司 | Composite microbial additive for feed and preparation method of composite microbial additive |
| CN102719380A (en) * | 2012-06-15 | 2012-10-10 | 天津昌农科技有限责任公司 | Aquatic compound microecologics and preparation method thereof |
| CN103468613A (en) * | 2013-09-12 | 2013-12-25 | 江苏省苏微微生物研究有限公司 | Bacillus megatherium, method for preparing microbial inoculum through solid fermentation of bacillus megatherium and application of microbial inoculum |
| CN103981118A (en) * | 2013-12-24 | 2014-08-13 | 北京伟嘉人生物技术有限公司 | Bacillus subtilis feed additive and preparation method and application thereof |
-
2015
- 2015-04-20 CN CN201510184402.6A patent/CN104738308A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2168909C1 (en) * | 2000-04-14 | 2001-06-20 | Общество с ограниченной ответственностью "БИОТРОФ" | Strain of bacterium lactobacillus plantarum for fodder ensilage |
| CN102409007A (en) * | 2011-07-04 | 2012-04-11 | 天津市育琪生物技术有限公司 | Bacillus microecological preparation and liquid-solid fermentation combining preparation process thereof |
| CN102524534A (en) * | 2011-08-03 | 2012-07-04 | 黑龙江省富天力生物科技有限公司 | Composite microbial additive for feed and preparation method of composite microbial additive |
| CN102409015A (en) * | 2011-12-06 | 2012-04-11 | 北京大北农科技集团股份有限公司 | Composite micro-ecological preparation as well as premixed material and application of preparation in feed additive |
| CN102719380A (en) * | 2012-06-15 | 2012-10-10 | 天津昌农科技有限责任公司 | Aquatic compound microecologics and preparation method thereof |
| CN103468613A (en) * | 2013-09-12 | 2013-12-25 | 江苏省苏微微生物研究有限公司 | Bacillus megatherium, method for preparing microbial inoculum through solid fermentation of bacillus megatherium and application of microbial inoculum |
| CN103981118A (en) * | 2013-12-24 | 2014-08-13 | 北京伟嘉人生物技术有限公司 | Bacillus subtilis feed additive and preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 李瑞瑞: "微生态制剂在畜牧上的应用", 《饲料研究》 * |
| 王金合: "微生态饲料添加剂及其在畜禽生产中的应用研究现状", 《畜牧与饲料科学》 * |
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