CN104737986A - Optimized solid medium for producing entomopathogenic nematodes - Google Patents
Optimized solid medium for producing entomopathogenic nematodes Download PDFInfo
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- CN104737986A CN104737986A CN201310749476.0A CN201310749476A CN104737986A CN 104737986 A CN104737986 A CN 104737986A CN 201310749476 A CN201310749476 A CN 201310749476A CN 104737986 A CN104737986 A CN 104737986A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Animal Behavior & Ethology (AREA)
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- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides an optimized solid medium in the orthogonal optimization design for producing entomopathogenic nematodes. By the use of the optimized solid medium, yield of the entomopathogenic nematodes in solid cultivation method is remarkably increased; the actual problems of contamination proneness of the medium and low yield during solid cultivation of the entomopathogenic nematodes at aseptic condition are solved; the solid entomopathogenic nematodes cultivation method is lower in cost and facilitates promotion and application of the entomopathogenic nematode products.
Description
Technical field
The present invention relates to optimization solid culture method, improve the method for entomopathogenic nematode output.
Background technology
According to the World Health Organization and UNEP's report, the whole world has more than 100 ten thousand people's pesticide poisonings every year, and wherein 20,000 people are dead.There are 6.7 ten thousand pesticide poisoning accidents in the U.S., in developing country, situation is even more serious every year.
China's pesticide in vegetable pollutes the livelihood issues having become a national strong interest, and especially on China leek, agricultural chemicals recall rate and exceeding standard rate are all more than 30%, and have pesticide poisoning accident to occur repeatly, food-safety problem is very troubling.As everyone knows, long-term high frequency time uses a large amount of uses of chemical pesticide can accelerate insect is developed immunity to drugs, and result causes peasant household can only excessive use or use highly toxic agricultural chemicals on the contrary, thus is absorbed in the vicious circle of pest control.
Entomopathogenic nematode is natural enemies of insects, and it is as a kind of desirable biological insecticides, and have the following advantages: obligatory parasitism, host range is wide; To host insect, there is initiatively search capability; Can enter in host insect body from the natural opening of host insect (as mouthpart, valve and anus), wound or intersegmental membrane, and host insect can be killed rapidly; Can at synthetic medium low cost mass propgation; Can use, to people and animals, plant and beneficial organism and Environmental security with number of chemical pestsides synthesis when field is used.Because entomopathogenic nematode belongs to environmentally friendly biological natural enemy, more American-European countries have exempted registration and have produced and production and sales, and have been widely used among the soil insect even control of ground insect.
The application process of current entomopathogenic nematode is mainly and is sprayed onto by solid or liquid culture nematode suspension out on crops or fruit tree.Solid matrix cultivation can produce entomopathogenic nematode, early eighties, sponge is made filler and adds people's chicken organ or other pluck materials respectively and add soybean meal, yeast extract, corn wet goods by Bedding and Wouts, the solid matrix achieving nematode is cultivated, improve entomopathogenic nematode output greatly, make nematode preparation production scale ascending.Meanwhile, with the filler of sponge as culture of nematodes, the solid culture of nematode is made to have moved towards batch production.Solid culture medium method produces entomopathogenic nematode, needs scale of investment little, needs labour more, belong to labor-intensive technology, is relatively applicable to the current national conditions of China.But when solid culture medium method cultivates nematode, production process needs aseptic, needs stable output, and then just can reduce costs, and improves the competitiveness of entomopathogenic nematode, wider and area substitutes chemical pesticide control harmful insect.
How existing formula being improved, improve solid process and produce entomopathogenic nematode, to improving and further genralrlization solid process production entomopathogenic nematode technology, there is very real investigation and application meaning.
Summary of the invention
Entomopathogenic nematode solid culture medium comprises the nutrient component such as carbon source, nitrogenous source, mainly comprises bean powder, flour, dried hen egg yolk, olive oil, yeast extract, sponge and water.The object of the invention is Optimal Medium, obtain output metastable entomopathogenic nematode solid culture based formulas.By the medium optimized, we obtain entomopathogenic nematode output 220,000-32 ten thousand nematode/gram medium, and this formula in the training period, do not have microbiological contamination phenomenon.The solid culture medium optimized in the present invention is that one can steady production entomopathogenic nematode solid culture medium.To the risk that in incubation, miscellaneous bacteria infects be reduced, effect stability.
The present invention utilizes the solid culture medium of optimization to produce entomopathogenic nematode.Nematode of the present invention is entomopathogenic nematode.
The solid culture of the production entomopathogenic nematode optimized in the present invention is:
The solid culture based formulas optimized: every 12 grams of medium comprise bean powder (1.79 grams), flour (0.31 gram), dried hen egg yolk (0.1 gram), olive oil (0.3 gram), yeast extract (0.2 gram), sponge (1.0 grams) and water (8.3 grams).
The entomopathogenic nematode solid culture medium that embodiment 1.12 grams is optimized
With bean powder (1.79 grams), flour (0.31 gram), dried hen egg yolk (01 gram), olive oil (0.3 gram), yeast extract (0.2 gram), sponge (1.0 grams) and water (8.3 grams) mixed configuration obtain 12 grams of grams of body medium.
The entomopathogenic nematode solid culture medium that embodiment 2.36 grams is optimized
With bean powder (5.37 grams), flour (0.93 gram), dried hen egg yolk (0.3 gram), olive oil (0.9 gram), yeast extract (0.6 gram), sponge (3.0 grams) and water (24.9 grams) mixed configuration obtain 36 grams of solid culture mediums.
The solid culture medium of embodiment 3. optimizing application increases the output of entomopathogenic nematode
Described in embodiment 1, configure 12 grams of solid culture mediums optimized, and traditionally aseptic culture step cultivates entomopathogenic nematode, effect of increasing production is obvious, and output reaches 300,000/gram of medium, adds 37% (table 1) than contrast.Control medium adopts literature reported method configuration (Wang Lifang etc. 2010).
Table 1, the solid culture based formulas optimized increase the effect (unit: bar/gram medium) of entomopathogenic nematode output
Described in embodiment 2, configure 36 grams of solid culture mediums optimized, traditionally aseptic culture step cultivates entomopathogenic nematode, and the output of Optimal Medium is about 23.7 ten thousand/gram of medium, adds 46% than contrast (12.7 ten thousand/gram of medium).There is miscellaneous bacteria in control group, and Optimal Medium group stable yield, there is good effect of increasing production (table 2).Control medium adopts literature reported method configuration (Wang Lifang etc. 2010).The medium of visible optimization can be stable production entomopathogenic nematode, be conducive to the suitability for industrialized production entomopathogenic nematode utilizing solid culture medium method.
Table 2, the solid culture based formulas optimized increase the effect (unit: bar/gram medium) of entomopathogenic nematode output
Bibliography:
Wang Lifang, Li Chunjie are perhaps gorgeous. the technical research of heterorhabditis indica in-vitro propagate. agricultural system science and comprehensive study, 2010,26 (1) 31-35.
Claims (2)
1. the solid culture medium of the production entomopathogenic nematode optimized.It is characterized in that the medium optimized is: in every 12 grams of medium, comprise bean powder (1.79 grams), flour (0.31 gram), dried hen egg yolk (0.1 gram), olive oil (0.3 gram), yeast extract (0.2 gram), sponge (1.0 grams) and water (8.3 grams).
2. the solid culture medium producing entomopathogenic nematode comprises olive oil, it is characterized in that adding olive oil in solid culture medium, significantly improves the output of entomopathogenic nematode.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310749476.0A CN104737986A (en) | 2013-12-30 | 2013-12-30 | Optimized solid medium for producing entomopathogenic nematodes |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310749476.0A CN104737986A (en) | 2013-12-30 | 2013-12-30 | Optimized solid medium for producing entomopathogenic nematodes |
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| CN104737986A true CN104737986A (en) | 2015-07-01 |
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| CN201310749476.0A Pending CN104737986A (en) | 2013-12-30 | 2013-12-30 | Optimized solid medium for producing entomopathogenic nematodes |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105815277A (en) * | 2016-03-22 | 2016-08-03 | 浙江绿盾生物科技有限公司 | Solid culture method for entomopathogenic nematodes |
| CN109430247A (en) * | 2018-11-21 | 2019-03-08 | 河南科技学院 | A kind of store method of Insectivorous nematode moisturizer and Insectivorous nematode |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06276892A (en) * | 1993-03-25 | 1994-10-04 | New Oji Paper Co Ltd | Culture medium for proliferating insect-parasitic nematode |
| CN1995329A (en) * | 2006-10-17 | 2007-07-11 | 广东省昆虫研究所 | Bacteria strain (X-7 strain) for culturing bait eelworm and its eelworm culture method |
| CN101041810A (en) * | 2006-10-17 | 2007-09-26 | 广东省昆虫研究所 | Bacteria strain (KG strain) for culturing bait nematode and nematode culture method |
| CN102972446A (en) * | 2012-11-22 | 2013-03-20 | 北京市西山试验林场 | Preparation method of steinernema carpocapsae |
-
2013
- 2013-12-30 CN CN201310749476.0A patent/CN104737986A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06276892A (en) * | 1993-03-25 | 1994-10-04 | New Oji Paper Co Ltd | Culture medium for proliferating insect-parasitic nematode |
| CN1995329A (en) * | 2006-10-17 | 2007-07-11 | 广东省昆虫研究所 | Bacteria strain (X-7 strain) for culturing bait eelworm and its eelworm culture method |
| CN101041810A (en) * | 2006-10-17 | 2007-09-26 | 广东省昆虫研究所 | Bacteria strain (KG strain) for culturing bait nematode and nematode culture method |
| CN102972446A (en) * | 2012-11-22 | 2013-03-20 | 北京市西山试验林场 | Preparation method of steinernema carpocapsae |
Non-Patent Citations (1)
| Title |
|---|
| 喻子牛等: "《农业微生物研究及产业化进展》", 29 February 2004, 中国农业出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105815277A (en) * | 2016-03-22 | 2016-08-03 | 浙江绿盾生物科技有限公司 | Solid culture method for entomopathogenic nematodes |
| CN109430247A (en) * | 2018-11-21 | 2019-03-08 | 河南科技学院 | A kind of store method of Insectivorous nematode moisturizer and Insectivorous nematode |
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