CN104726503A - Method for producing erythritol in fermentation tank - Google Patents
Method for producing erythritol in fermentation tank Download PDFInfo
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- CN104726503A CN104726503A CN201510156469.9A CN201510156469A CN104726503A CN 104726503 A CN104726503 A CN 104726503A CN 201510156469 A CN201510156469 A CN 201510156469A CN 104726503 A CN104726503 A CN 104726503A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
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Abstract
The invention relates to the field of microorganisms, and particularly relates to a method for producing erythritol by inoculating in a fermentation tank instead of seed flasks. The method disclosed by the invention comprises the following steps: inoculating candida lipolytica for producing erythritol to multiple eggplant flasks, wherein an inclined culture medium comprises the following components in parts by weight: 150-250 parts of glucose, 5-15 parts of a yeast extract, 0.5-1.5 parts of urea, 15-25 parts of agal-agal, and 800-1200 parts of tap water; after the pH value is adjusted to 6.0, culturing for 45-50 h at a temperature of 28-32 DEG C, and adding 80-120 parts of sterile water in each eggplant flask; and inoculating all lawns obtained by washing to a sterilized fermentation tank containing 20 L of the culture medium to culture for 15-22 h, completely inoculating the obtained objects to a primary seed flask, and then enabling the obtained products to pass through a secondary seed flask and the fermentation tank, so that erythritol is produced. The method disclosed by the invention is designed for producing erythritol by inoculating in the fermentation tank instead of seed flasks, thereby saving manpower and time, and greatly reducing the incidence of bacterial infection.
Description
Technical field
The invention belongs to fermentation technical field, be specifically related to a kind of method that erythritol produced by fermentor tank.
Background technology
Erythritol is the sweeting agent of natural zero-calorie, has refrigerant sense, special taste time edible.Due to erythritol not involved in sugar metabolism and change of blood sugar, diabetic patients.The unlikely carious tooth of erythritol, dosis tolerata is higher than Xylitol, absorbs fast, almost without the possibility causing gastrointestinal upset.In edible sugar alcohol, erythritol uniquely adopts fermentation method to produce, closer to natural transformation and extraction.Along with growth in the living standard, people more and more focus on health care of food, and erythritol is as the superior health promoting candy of a kind of quality, and its demand will increase progressively throughout the year.At present, erythritol bacterial classification majority is produced in domestic fermentation is Candida lipolytica.Traditional method roughly flow process is: and seed slant culture → inclined-plane turns shaking flask and spreads cultivation → and shaking flask bacterium liquid is merged into seed bottle → first order seed enlarged culturing → secondary seed enlarged culturing → fermentation → extraction erythritol.Because first order seed inoculum size is large, seed bottle generally need merge 200 shaking flasks, and the operating time is long, takes time and effort and microbiological contamination probability is high.
Summary of the invention
In order to solve above-mentioned technical problem, the invention provides a kind of operating time short, save time and muscle power and effectively can reduce the method for microbiological contamination probability.
The method of erythritol produced by fermentor tank, comprises the steps:
The Candida lipolytica producing erythritol is inoculated in multiple eggplant bottle, slant medium is the composition of following portions by weight: glucose 150-250 part, yeast extract paste 5-15 part, urea 0.5-1.5 part, agar 15-25 part, tap water 800-1200 part, adjust ph is 6.0, in each eggplant bottle, the sterilized water of 80-120 part is added cultivate 45-50h at 28-32 DEG C after, that the lawn under washing all is accessed sterilizing cultivates 15-22h containing in 20L culture medium fermentation tank, then all access first class seed pot, then produce erythritol through secondary seed tank, fermentor tank.
Preferably, the method for erythritol produced by above-mentioned fermentor tank, comprises the steps:
The Candida lipolytica producing erythritol is inoculated in 10 eggplant bottles, slant culture based component is: glucose 200g, yeast extract paste 10g, urea 1.0g, agar 20g, tap water 1000mL, pH6.0, to each eggplant bottle in adds 100mL sterilized water after cultivating 48h at 30 DEG C, and that the lawn under washing all is accessed sterilizing cultivates 18h containing in 20L culture medium fermentation tank, then all access first class seed pot, then produce erythritol through secondary seed tank, fermentor tank.
In above-mentioned fermentor tank, the composition of substratum is: glucose 210 parts, yeast extract paste 11 parts, 1.1 parts, urea, thiamine hydrochloride 5 × 10
-3part, vitamin H 0.12 × 10
-3part, calcium chloride 29 × 10
-3, ferrous sulfate 15 × 10
-3, 1000 parts, tap water, 0.2 part of defoamer, pH value is 6.2.
After eggplant bottle graft kind fermentor tank, at rotating speed 300-800r/min, air flow 0.5-1.0VVM, tank pressure 0.8Mpa, dissolved oxygen 30%, temperature 30 DEG C, cultivates 18h under pH6.0 condition, all accesses first class seed pot.
After eggplant bottle graft kind fermentor tank, 0-3h rotating speed controls progressively to be promoted to 800r/min for 300r/min, 3-10h rotating speed.
After eggplant bottle graft kind fermentor tank, 0-3h control air flow is 0.5VVM, 3-12 hour control air flow is 0.5-1.0VVM.
Fermentor tank thief hole is connected with first class seed pot.
When transferred species, close fermentor tank vent valve, and keep fermentor tank tank internal pressure to be greater than first class seed pot.
Beneficial effect of the present invention is, adopts method fermentor tank of the present invention to replace seed bottle to prepare seed liquor, produces erythritol, not only save human time but also greatly reduce microbiological contamination probability.And method of the present invention produces erythritol, prepares seed liquor relative to existing seed bottle, produce erythritol, under the condition reducing energy consumption, manpower and time loss, output will exceed 1%-2%.
Embodiment
Below in conjunction with specific embodiment, the present invention is further described, so that those skilled in the art more understands the present invention, but does not therefore limit the present invention.
Embodiment 1
Be inoculated in 10 eggplant bottles that (slant culture based component is: glucose 200g by producing the Candida lipolytica of erythritol, yeast extract paste 10g, urea 1.0g, agar 20g, tap water 1000mL, pH6.0), in each eggplant bottle, 100mL sterilized water is added after 30 DEG C of cultivation 48h, the lawn under washing all access through sterilizing containing the fermentor tank of 20L substratum in cultivate 18h, then all first class seed pot is accessed, inoculum size is 1%, secondary seed tank is accessed after 20h, inoculum size is 10%, fermentor tank is accessed after 10h, inoculum size is 10%, recording erythritol content after fermentation 120h is 160g/L.
Comparative example 1
Be inoculated in 10 eggplant bottles that (slant culture based component is: glucose 200g by producing the Candida lipolytica of erythritol, yeast extract paste 10g, urea 1.0g, agar 20g, tap water 1000mL, pH6.0), in each eggplant bottle, 100mL sterilized water is added after 30 DEG C of cultivation 48h, lawn under washing all is accessed in 200 1000mL shaking flasks, each shaking flask liquid amount is 100mL, access 5mL bacterium liquid, 30 DEG C, 180r/min is all merged in a seed bottle after cultivating 24h, seed bottle bacterium liquid all accesses first class seed pot, inoculum size is 1%, secondary seed tank is accessed after 20h, inoculum size is 10%, fermentor tank is accessed after 10h, inoculum size is 10%, recording erythritol content after fermentation 120h is 157g/L.
As can be seen from the above table, adopt method of the present invention to inoculate, Shake flask medium spreads cultivation 18 hours, and erythritol output reaches 160g/L;
Adopt the method inoculation of seed bottle graft kind first class seed pot, Shake flask medium spreads cultivation 24 h, and erythritol output just reaches 157g/L; Method of the present invention is not only saved time relative to the method in comparative example 1, and erythritol output is higher than comparative example 1.
Embodiment 2
As different from Example 1, in embodiment 2, the Candida lipolytica producing erythritol is inoculated in multiple eggplant bottle, slant medium is the composition of following portions by weight: glucose 150 parts, yeast extract paste 5 parts, 0.5 part, urea, 15 parts, agar, 800 parts, tap water, adjust ph is 6.0, to each eggplant bottle in adds 100mL sterilized water after cultivating 45h at 28 DEG C, and that the lawn under washing all is accessed sterilizing cultivates 19h containing in 20L culture medium fermentation tank, whole access first class seed pot, then produce erythritol through secondary seed tank, fermentor tank.
Embodiment 3
As different from Example 1, in embodiment 2, the Candida lipolytica producing erythritol is inoculated in multiple eggplant bottle, slant medium is the composition of following portions by weight: glucose 250 parts, yeast extract paste 15 parts, 1.5 parts, urea, 25 parts, agar, 1200 parts, tap water, adjust ph is 6.0, to each eggplant bottle in adds 100mL sterilized water after cultivating 50h at 32 DEG C, and that the lawn under washing all is accessed sterilizing cultivates 18h containing in 20L culture medium fermentation tank, whole access first class seed pot, then produce erythritol through secondary seed tank, fermentor tank.
Claims (9)
1. the method for erythritol produced by fermentor tank, comprises the steps:
The Candida lipolytica producing erythritol is inoculated in multiple eggplant bottle, slant medium is the composition of following portions by weight: glucose 150-250 part, yeast extract paste 5-15 part, urea 0.5-1.5 part, agar 15-25 part, tap water 800-1200 part, adjust ph is 6.0, in each eggplant bottle, the sterilized water of 80-120 part is added cultivate 45-50h at 28-32 DEG C after, that the lawn under washing all is accessed sterilizing cultivates 15-22h containing in 20L culture medium fermentation tank, whole access first class seed pot, again through secondary seed tank, fermentor tank produces erythritol, above-mentioned part is parts by weight.
2. the method for erythritol produced by fermentor tank as claimed in claim 1, comprises the steps:
The Candida lipolytica producing erythritol is inoculated in 10 eggplant bottles, slant culture based component is: glucose 200g, yeast extract paste 10g, urea 1.0g, agar 20g, tap water 1000mL, pH6.0, to each eggplant bottle in adds 100mL sterilized water after cultivating 48h at 30 DEG C, and that the lawn under washing all is accessed sterilizing cultivates 18h containing in the fermentor tank of 20L substratum, whole access first class seed pot, then produce erythritol through secondary seed tank, fermentor tank.
3. the method for erythritol produced by the fermentor tank according to any one of claim 1-2, it is characterized in that: in fermentor tank, the composition of substratum is: glucose 210 parts, yeast extract paste 11 parts, 1.1 parts, urea, thiamine hydrochloride 5 × 10
-3part, vitamin H 0.12 × 10
-3part, calcium chloride 29 × 10
-3, ferrous sulfate 15 × 10
-3, 1000 parts, tap water, 0.2 part of defoamer, pH value is 6.2.
4. the method for erythritol produced by fermentor tank as claimed in claim 3, it is characterized in that: after eggplant bottle graft kind fermentor tank, at rotating speed 300-800r/min, air flow 0.5-1.0VVM, tank pressure 0.8Mpa, dissolved oxygen 30%, temperature 28-32 DEG C, cultivate 18h under pH5.5-6.5 condition, all access first class seed pot.
5. the method for erythritol produced by fermentor tank as claimed in claim 4, it is characterized in that: after eggplant bottle graft kind fermentor tank, at rotating speed 300-800r/min, air flow 0.5-1.0VVM, tank pressure 0.8Mpa, dissolved oxygen 30%, temperature 30 DEG C, cultivates 18h under pH6.0 condition, all accesses first class seed pot.
6. the method for erythritol produced by fermentor tank as claimed in claim 5, it is characterized in that: after eggplant bottle graft kind fermentor tank, and 0-3h rotating speed controls progressively to be promoted to 800r/min for 300r/min, 3-10h rotating speed.
7. the method for erythritol produced by fermentor tank as claimed in claim 6, it is characterized in that: after eggplant bottle graft kind fermentor tank, and 0-3h control air flow is 0.5VVM, 3-12 hour control air flow is 0.5-1.0VVM.
8. the method for erythritol produced by fermentor tank as claimed in claim 7, it is characterized in that: fermentor tank thief hole is connected with first class seed pot.
9. the method for erythritol produced by fermentor tank as claimed in claim 8, it is characterized in that: during transferred species, closes fermentor tank vent valve, and keeps fermentor tank tank internal pressure to be greater than first class seed pot.
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| CN201510156469.9A CN104726503A (en) | 2015-04-03 | 2015-04-03 | Method for producing erythritol in fermentation tank |
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| CN201510156469.9A CN104726503A (en) | 2015-04-03 | 2015-04-03 | Method for producing erythritol in fermentation tank |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109504733A (en) * | 2018-12-18 | 2019-03-22 | 浙江华康药业股份有限公司 | A kind of preparation method of antierythrite |
| CN110564782A (en) * | 2019-09-23 | 2019-12-13 | 安徽丰原生物化学股份有限公司 | Erythritol fermentation production method |
| CN111424058A (en) * | 2020-04-24 | 2020-07-17 | 保龄宝生物股份有限公司 | Method for preparing erythritol by adopting continuous fermentation mode |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3756917A (en) * | 1971-11-12 | 1973-09-04 | Pfizer | Fermentation process for the production of erythritol |
| JPH05137585A (en) * | 1991-11-21 | 1993-06-01 | Mitsubishi Kasei Corp | Method for continuously culturing erythritol |
| CN1629299A (en) * | 2004-10-12 | 2005-06-22 | 山东保龄宝生物技术有限公司 | Process for preparing Erythritol through fermentation |
| CN1932002A (en) * | 2005-09-14 | 2007-03-21 | 中国食品发酵工业研究院 | Candida lipolytica and the erythritol producing process therewith |
| CN101302551A (en) * | 2008-06-11 | 2008-11-12 | 青岛琅琊台集团股份有限公司 | Antierythrite production method |
| CN102352393A (en) * | 2011-10-26 | 2012-02-15 | 山东福田药业有限公司 | Process for producing erythritol by utilizing Yarrowia lipolytica strains |
-
2015
- 2015-04-03 CN CN201510156469.9A patent/CN104726503A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3756917A (en) * | 1971-11-12 | 1973-09-04 | Pfizer | Fermentation process for the production of erythritol |
| JPH05137585A (en) * | 1991-11-21 | 1993-06-01 | Mitsubishi Kasei Corp | Method for continuously culturing erythritol |
| CN1629299A (en) * | 2004-10-12 | 2005-06-22 | 山东保龄宝生物技术有限公司 | Process for preparing Erythritol through fermentation |
| CN1932002A (en) * | 2005-09-14 | 2007-03-21 | 中国食品发酵工业研究院 | Candida lipolytica and the erythritol producing process therewith |
| CN101302551A (en) * | 2008-06-11 | 2008-11-12 | 青岛琅琊台集团股份有限公司 | Antierythrite production method |
| CN102352393A (en) * | 2011-10-26 | 2012-02-15 | 山东福田药业有限公司 | Process for producing erythritol by utilizing Yarrowia lipolytica strains |
Non-Patent Citations (3)
| Title |
|---|
| 中财网: "保龄宝(002286)年产4000吨赤藓糖醇易地技改项目可行性报告", 《HTTP://WWW.CFI.NET.CN/P20100126000764.HTML》 * |
| 刘如林: "《微生物工程概论》", 31 October 1995, 南开大学出版社 * |
| 山东食品发酵工程设计所: "食品发酵工程设计所2009年完成工程设计咨询项目一览表", 《HTTP://WWW.SPGCSJ.COM/NEWS2_DETAIL.ASP?ID=28》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109504733A (en) * | 2018-12-18 | 2019-03-22 | 浙江华康药业股份有限公司 | A kind of preparation method of antierythrite |
| CN110564782A (en) * | 2019-09-23 | 2019-12-13 | 安徽丰原生物化学股份有限公司 | Erythritol fermentation production method |
| CN111424058A (en) * | 2020-04-24 | 2020-07-17 | 保龄宝生物股份有限公司 | Method for preparing erythritol by adopting continuous fermentation mode |
| CN111424058B (en) * | 2020-04-24 | 2022-02-18 | 保龄宝生物股份有限公司 | Method for preparing erythritol by adopting continuous fermentation mode |
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