CN101302551A

CN101302551A - Antierythrite production method

Info

Publication number: CN101302551A
Application number: CNA2008100168100A
Authority: CN
Inventors: 李悦明; 张希铭; 徐建春; 孙慧彬; 刘同照; 陈金凤; 曲文强
Original assignee: QINGDAO LANGYATAI (GROUP) CO Ltd
Current assignee: QINGDAO LANGYATAI (GROUP) CO Ltd
Priority date: 2008-06-11
Filing date: 2008-06-11
Publication date: 2008-11-12

Abstract

The invention discloses a method for producing erythritol. Glucose is taken as raw material, candidalipolytica is taken as a fermentation strain, shaking culture as well as first-stage and second-stage inoculum development are performed, then the fermentation in a fermentation tank is carried out, mycoprotein impurities in zymotic fluid are removed by a ceramic membrane after the fermentation is finished, then a sodium membrane is used to selectively pass through the erythritol so as to extract the erythritol, and finally the erythritol product is obtained through condensation crystallization and separation drying. The method has the advantages of short incubation period, high extraction yield, low production cost and so on.

Description

A kind of erythritol production method

Technical field

The present invention relates to a kind of method of cultivation of erythritol.

Background technology

Erythritol is a kind of four carbon polyols that not only contained sweet taste but also empty calory.It extensively is present in marine alga, mushroom, melon, grape, pears and the leavened food; Simultaneously, also be present in the tissue and body fluid of humans and animals.Erythritol has low energy value, high dosis tolerata, has no side effect, diabetics's edible, the non-advantageous characteristic such as carious tooth that cause, and extremely is fit to be applied in the functional foodstuff.The preparation method of erythritol is mainly microbe fermentation method, and its suitability for industrialized production is to be raw material with starch, is formed through fermentation by osmophilic yeast.Present production method, fermentation period adopts Plate Filtration technology, the long control that is unfavorable for fermenting process to microbiological contamination of fermentation period more than 150 hours when fermented liquid separates, the Plate Filtration technical point is unfavorable for the removing of impurity such as tropina from fermented liquid, and extraction yield has only 75%.

Summary of the invention

Task of the present invention is to provide a kind of erythritol production method, and this method has fermentation period weak point, good separating effect, extraction yield advantages of higher.

Its technical solution is:

With glucose is raw material, and Candida lipolytica is a fermentation strain, through shaking table cultivation and one-level, secondary seed enlarged culturing, carries out ferment tank then, 25-35 ℃ of jar temperature, and tank pressure 0-0.1MPa, dissolved oxygen 20%-80% saturation ratio is at C ₆H ₁₂O ₆H ₂The O initial content is 20-40%, inorganic salt MgSO ₄7H ₂O content is 0.04-0.08%, (NH ₄) ₂SO ₄Content is 0.04-0.06%, KH ₂PO ₄Content is 0.10-0.15%, CuSO ₄Content is 0.04-0.06%, yeast extract paste content is to ferment under the condition of 0.80-0.90%, fermentation period 100-120 hour, fermented liquid is removed tropina impurity with ceramic membrane, pass through erythritol with sodium film selectivity then, erythritol is extracted, and last condensing crystal, separation drying obtain the erythritol product.

The invention has the beneficial effects as follows: shortened fermentation period, help the control of fermenting process to microbiological contamination, guarantee the quality of fermented liquid, subsequent handlings such as the filtration ground that more helps fermented liquid carries out, and adopts membrane separation technique can improve the clarity of feed liquid, reduces because of the extraction of factor affecting products such as impurity and the quality of product, mother liquor after separating in addition can be got back to ferment tank once more, make full use of the glucose in the mother liquor, improved utilization ratio of raw materials, reduced production cost.

Embodiment

For the ease of understanding the present invention, come more detailed description the present invention below in conjunction with embodiment.Yet these embodiment just play the effect of explanation, and the present invention is not limited to these embodiment.

Embodiment 1: be raw material with glucose, Candida lipolytica is a fermentation strain, through shaking table cultivation and one-level, secondary seed enlarged culturing, carries out ferment tank at last, 25 ℃ of jar temperature, and tank pressure 0MPa, dissolved oxygen 20% saturation ratio is at C ₆H ₁₂O ₆H ₂The O initial content is 20%, inorganic salt MgSO ₄7H ₂O content is 0.04%, (NH ₄) ₂SO ₄Content is 0.04%, KH ₂PO ₄Content is 0.10%, CuSO ₄Content is 0.04%, yeast extract paste content is to ferment under 0.80% the condition, fermentation period is 100 hours, fermented liquid is removed impurity such as tropina with ceramic membrane, pass through erythritol with U.S.'s import sodium film selectivity then, erythritol is extracted, and last condensing crystal, separation drying obtain the erythritol product.After tested, the sugar degree of erythritol is 0.07%, and extraction yield is 80%.

Embodiment 2: be raw material with glucose, Candida lipolytica is a fermentation strain, through shaking table cultivation and one-level, secondary seed enlarged culturing, carries out ferment tank at last, 30 ℃ of jar temperature, and tank pressure 0.05MPa, dissolved oxygen 50% saturation ratio is at C ₆H ₁₂O ₆H ₂The O initial content is 30%, inorganic salt MgSO ₄7H ₂O content is 0.06%, (NH ₄) ₂SO ₄Content is 0.05%, KH ₂PO ₄Content is 0.13%, CuSO ₄Content is 0.05%, yeast extract paste content is to ferment under 0.86% the condition, fermentation period is 110 hours, fermented liquid is removed impurity such as tropina with ceramic membrane, pass through erythritol with U.S.'s import sodium film selectivity then, erythritol is extracted, and last condensing crystal, separation drying obtain the erythritol product.After tested, the sugar degree of erythritol is 0.06%, and extraction yield is 82%.

Embodiment 3: be raw material with glucose, Candida lipolytica is a fermentation strain, through shaking table cultivation and one-level, secondary seed enlarged culturing, carries out ferment tank at last, 35 ℃ of jar temperature, and tank pressure 0.1MPa, dissolved oxygen 80% saturation ratio is at C ₆H ₁₂O ₆H ₂The O initial content is 40%, inorganic salt MgSO ₄7H ₂O content is 0.08%, (NH ₄) ₂SO ₄Content is 0.06%, KH ₂PO ₄Content is 0.15%, CuSO ₄Content is 0.06%, yeast extract paste content is to ferment under 0.90% the condition, fermentation period is 120 hours, fermented liquid is removed impurity such as tropina with ceramic membrane, pass through erythritol with U.S.'s import sodium film selectivity then, erythritol is extracted, and last condensing crystal, separation drying obtain the erythritol product.After tested, the sugar degree of erythritol is 0.08%, and extraction yield is 81%.

Claims

1, a kind of erythritol production method, it is characterized in that: be raw material with glucose, Candida lipolytica is a fermentation strain, through shaking table cultivation and one-level, secondary seed enlarged culturing, carry out ferment tank then, at 25-35 ℃ of jar temperature, tank pressure 0-0.1MPa, dissolved oxygen 20%-80% saturation ratio is at C ₆H ₁₂O ₆H ₂The O initial content is 20-40%, inorganic salt MgSO ₄7H ₂O content is 0.04-0.08%, (NH ₄) ₂SO ₄Content is 0.04-0.06%, KH ₂PO ₄Content is 0.10-0.15%, CuSO ₄Content is 0.04-0.06%, yeast extract paste content is to ferment under the condition of 0.80-0.90%, fermentation period 100-120 hour, fermented liquid is removed tropina impurity with ceramic membrane, pass through erythritol with sodium film selectivity then, erythritol is extracted, and last condensing crystal, separation drying obtain the erythritol product.