CN107217020A - It is a kind of suitable for culture medium of lactobacillus acidophilus and preparation method thereof - Google Patents
It is a kind of suitable for culture medium of lactobacillus acidophilus and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to biological technical field, and in particular to a kind of suitable for culture medium of lactobacillus acidophilus and preparation method thereof, be made up of the composition of following weight：1000~2000ml of distilled water, 0.2~1.0g/L of magnesium sulfate, 0.1~0.5g/L of manganese sulfate, 10~40g/L of yeast extract, 1.0~4.0g/L of dipotassium hydrogen phosphate, 1.0~4.0g/L of diammonium hydrogen citrate, 5~20g/L of beef extract, 10~20g/L of agar, 5~20g/L of peptone, 0.5~2g/L of Tween 80,50~200g/L of tomato juice, 7.5~30g/L of sodium acetate, 0.04~0.2g/L of 10~40g/L of glucose and dandelion extract.The culture medium is particularly suitable for use in the culture of lactobacillus acidophilus, it can significantly improve the viable count of lactobacillus acidophilus, substantially shorten the time that lactobacillus acidophilus enters stationary phase, and due to the addition of native factor, preparation-obtained lactobacillus acidophilus also contains substantial amounts of beneficial metabolic product.
Description
Technical field
The invention belongs to biological technical field, and in particular to a kind of culture medium and its preparation side suitable for lactobacillus acidophilus
Method.
Background technology
Lactobacillus acidophilus (Lactobacillus acidophilus) belongs to lactobacillus, can pass through carbon aquation of fermenting
Compound produces lactic acid, and it is widely present in the enteron aisle of people and animal, to the health of human body, particularly to maintaining intestines and stomach just
Normal physiological function plays an important roll, and is beneficial microbe colony important in enteron aisle.
It is living in lactobacillus acidophilus product in the market because lactobacillus acidophilus own growth condition requires more special
Bacterial content is relatively low.Therefore, people are trying to explore the material that it can be promoted to grow always, to improve the work of lactobacillus acidophilus
Bacterium number.Meanwhile, lactobacillus acidophilus be separately cultured and its enzymology is required for good enriched medium, make the newborn bar of acidophilus
Bacterium is able to largely breed within the shortest time, so that it still has enough viable counts after a series of subsequent operation.
Therefore, the optimization of the enriched medium of lactobacillus acidophilus turns into a current urgent problem.
Chinese patent application CN103255093B discloses a kind of preparation method of lactobacillus acidophilus, and it is related to a kind of liquid
Culture medium, specific composition be the weight % of glucose 2, the weight % of peptone 1, the weight % of beef extract 1, the weight % of yeast extract 0.5,
Dipotassium hydrogen phosphate 0.2 weight %, NaAc0.5 weight %, the weight % of magnesium sulfate 0.05, the weight % of manganese sulfate 0.025, tween-
800.1 volume %, the weight % of lemon acid diamine 0.2, the weight % of agar 1.5, the weight % of tomato juice 5.The culture medium is prepared
Lactobacillus acidophilus also with other a large amount of beneficial metabolic products：Lactein and Pfansteihl, n-butyric acie, acetic acid, formic acid etc. have
Machine acid.These products are the important energy sources of animal intestinal tract, and can be acted synergistically with probiotics, act on animal, can show
Write and improve the gastrointestinal function of animal, improve immunity, promote growth；The invention products therefrom is prepared into feed, animal pair
The digestion of feed and absorptivity are improved.But the viable count of lactobacillus acidophilus that the culture medium is obtained is relatively low, and acidophilus breast
The time that bacillus enters stationary phase is long.
Therefore, it is necessary to provide a kind of for promoting lactobacillus acidophilus to grow, and it can substantially shorten lactobacillus acidophilus entrance
The culture medium of time stationary phase.
The content of the invention
The present invention is intended to provide a kind of suitable for culture medium of lactobacillus acidophilus and preparation method thereof.The culture medium with the addition of
The spontaneous growth factor, tomato juice and dandelion extract, both can remarkably promote the growth and breeding of lactobacillus acidophilus, significantly carry
The viable count of high lactobacillus acidophilus, and can substantially shorten the time that lactobacillus acidophilus enters stationary phase.
In order to achieve the above object, the present invention uses following technical scheme：A kind of culture medium suitable for lactobacillus acidophilus,
It is made up of the composition of following weight：1000~2000ml of distilled water, 0.2~1.0g/L of magnesium sulfate, 0.1~0.5g/L of manganese sulfate,
10~40g/L of yeast extract, 1.0~4.0g/L of dipotassium hydrogen phosphate, 1.0~4.0g/L of diammonium hydrogen citrate, beef extract 5~
20g/L, 10~20g/L of agar, 5~20g/L of peptone, 0.5~2g/L of Tween 80,50~200g/L of tomato juice, sodium acetate 7.5
~30g/L, 0.04~0.2g/L of 10~40g/L of glucose and dandelion extract.
Further, the culture medium is made up of the composition of following weight：Distilled water 1000ml, magnesium sulfate 0.58g/L, sulphur
Sour manganese 0.25g/L, yeast extract 20g/L, dipotassium hydrogen phosphate 2g/L, diammonium hydrogen citrate 2g/L, beef extract 10g/L, agar
14g/L, peptone 10g/L, Tween 80 1g/L, tomato juice 150g/L, sodium acetate 15g/L, glucose 20g/L and dandelion carry
Take thing 0.1g/L.
Further, the tomato juice is made by following steps：
Fresh tomato is taken, purified water warm water 5~15min of blanching of 1~2 times of amount of tomato is added after clean, section, is squeezed the juice,
Filtering, regulation pH is 6.3,110~120 DEG C of 10~15min of sterilizing, is produced.
Further, the dandelion extract is made by following steps：Dandelion medicinal material is weighed, is crushed, medicinal material is added
The purified water of 10~20 times of amounts, is decocted 1~3 time, 0.5~1h, filtration, merging filtrate, are concentrated and dried, are produced after crushing every time.
Lactobacillus acidophilus has the pH scopes of suitable growth, when hydrogen ion concentration exceedes the adaptation of lactobacillus acidophilus in culture medium
After scope, the metabolism of lactobacillus acidophilus will be suppressed.When environment pH is in optimum range, lactobacillus acidophilus metabolic activity meeting
Enhancing, growth and breeding is accelerated, it is therefore preferred that the initial pH of the culture medium is 6.0~6.6, it is highly preferred that the culture medium
Initial pH be 6.2.
There is important influence to the growth and breeding of lactobacillus acidophilus as the temperature of one of condition of culture.In suitable temperature
Under the conditions of degree, the flourish of lactobacillus acidophilus faster, is conducive to the raising of the bacterium number of lactobacillus acidophilus.Within the specific limits,
The rise of temperature can improve the growth rate of cell, after more than optimal temperature, and the rise of temperature can be in microbial body
Protein, nucleic acid produce influence, then growth rate declines with the rise of temperature, are such as further continued for rise, then cell function is drastically
Decline down to dead.Therefore, the initial temperature of culture medium of the present invention is preferably 28~40 DEG C, more preferably 37 DEG C.
The size of inoculum concentration directly affects the growth and breeding speed of strain in incubation.Inoculum concentration is big, thalline lag phase
Shorten, propagation is rapid, but too fast breeding is easily caused nutriment in culture medium and lacked.Inoculum concentration is small, thalline adjustment period
Slow-growing, growth cycle extension easily increases microbiological contamination chance.Therefore, suitable inoculum concentration is to ensure lactic acid bacteria high efficiently multiplying
A key.Preferably, the inoculum concentration of lactobacillus acidophilus is 2~4%, more preferably 3%.
Further, the lactobacillus acidophilus is lactobacillus acidophilus JCM1132, and its deposit number is：CGMCC1.1878 phases
Ying Di, present invention also offers a kind of method for preparing the above-mentioned culture medium for lactobacillus acidophilus, comprises the following steps：
A) the preparation of tomato juice：Fresh tomato is taken, the purified water warm water blanching of 1~2 times of amount of tomato is added after clean, section
5~15min, squeezes the juice, filtering, and regulation pH is 6.3,110~120 DEG C of 10~15min of sterilizing, is placed in refrigerator preservation, standby；
B) the preparation of dandelion extract：Dandelion medicinal material is weighed, is crushed, the purified water of 10~20 times of amounts of medicinal material is added,
Decoct 1~3 time, 0.5~1h, filtration, merging filtrate, are concentrated and dried, are produced after crushing every time；
C each component) is weighed in proportion, is mixed, pH is adjusted, and high pressure moist heat sterilization is produced.
Another object of the present invention is to provide a kind of lactobacillus acidophilus cultural method, the strain of lactobacillus acidophilus of activation is pressed
2~4% inoculum concentration is accessed in culture medium as described above, is placed in 20~30h of culture in 37 DEG C of constant incubators, is produced.
The present invention has advantages below：
The present invention adds tomato juice on the basis of basal medium and the newborn bar of acidophilus of the present invention is made in dandelion extract
Bacterium culture medium, the culture medium is particularly suitable for use in the culture of lactobacillus acidophilus, and the culture medium can significantly improve lactobacillus acidophilus
Viable count, hence it is evident that shorten the time that lactobacillus acidophilus enters stationary phase, and due to the addition of native factor, it is preparation-obtained thermophilic
Lactobacillus lactis also contains substantial amounts of beneficial metabolic product.
Brief description of the drawings
Fig. 1 shows the influence that different growth factors grow to lactobacillus acidophilus；
Fig. 2 shows the influence that initial pH value of medium grows to lactobacillus acidophilus；
Fig. 3 shows the influence that inoculum concentration grows to lactobacillus acidophilus；
Fig. 4 shows the growth curve of lactobacillus acidophilus before and after medium optimization.
Embodiment
The embodiment of form, further specifically to the above work of the present invention by the following examples
It is bright.But the scope that this should not be interpreted as to above-mentioned theme of the invention is only limitted to following examples.
Culture medium prescription fractions of the present invention buy information
A kind of culture medium suitable for lactobacillus acidophilus of embodiment 1
The culture medium is made up of the composition of following weight：Distilled water 1000ml, magnesium sulfate 0.58g/L, manganese sulfate
0.25g/L, yeast extract 20g/L, dipotassium hydrogen phosphate 2g/L, diammonium hydrogen citrate 2g/L, beef extract 10g/L, agar 14g/
L, peptone 10g/L, Tween 80 1g/L, tomato juice 150g/L, sodium acetate 15g/L, glucose 20g/L and dandelion extract
0.1g/L。
Preparation method：
A) the preparation of tomato juice：Fresh tomato is taken, the purified water warm water blanching of 1 times of amount of tomato is added after clean, section
10min, squeezes the juice, filtering, and regulation pH is 6.3,115 DEG C of sterilizing 15min, is placed in refrigerator preservation, standby；
B) the preparation of dandelion extract：Dandelion medicinal material 20g is weighed, is crushed, the 500mL that adds water decoctions 2 times, every time
0.5h, filtration, merging filtrate is concentrated and dried, produced after crushing；
C each component) is weighed in proportion, is mixed, and regulation pH is 6.2, and high pressure moist heat sterilization is produced.
Embodiment 2, a kind of culture medium suitable for lactobacillus acidophilus
The culture medium is made up of the composition of following weight：Distilled water 1500ml, magnesium sulfate 0.2g/L, manganese sulfate 0.1g/
L, yeast extract 10g/L, dipotassium hydrogen phosphate 1.0g/L, diammonium hydrogen citrate 1.0g/L, beef extract 5g/L, agar 10g/L, egg
White peptone 5g/L, Tween 80 0.5g/L, tomato juice 100g/L, sodium acetate 7.5g/L, glucose 10g/L and dandelion extract
0.04g/L。
Preparation method reference implementation example 1.
Embodiment 3, a kind of culture medium suitable for lactobacillus acidophilus
The culture medium is made up of the composition of following weight：Distilled water 2000ml, magnesium sulfate 1.0g/L, manganese sulfate 0.5g/
L, yeast extract 40g/L, dipotassium hydrogen phosphate 4.0g/L, diammonium hydrogen citrate 4.0g/L, beef extract 20g/L, agar 20g/L,
Peptone 20g/L, Tween 80 2g/L, tomato juice 200g/L, sodium acetate 30g/L, glucose 40g/L and dandelion extract
0.2g/L。
Comparative example 1, a kind of culture medium suitable for lactobacillus acidophilus
The difference of comparative example 1 and embodiment 1 is, removes dandelion extract, and the weight of tomato juice is increased to
150.1g/L, remaining parameter and operation such as embodiment 1.
Comparative example 2, a kind of culture medium suitable for lactobacillus acidophilus
The difference of comparative example 2 and embodiment 1 is, removes tomato juice, and the weight of dandelion extract is increased to
150.1g/L, remaining parameter and operation such as embodiment 1.
Comparative example 3, a kind of culture medium suitable for lactobacillus acidophilus
The difference of comparative example 3 and embodiment 1 is, removes tomato juice and dandelion extract, uses carrot juice 150.1g/L
Substitute, remaining parameter and operation such as embodiment 1.
The preparation of carrot juice：
Take fresh carrot 200g, with purified water clean, section after plus 200mL purified water blanching 10min, smash with juice extractor
Broken to squeeze the juice, double gauze filtering juice adjusts after pH6.3,115 DEG C of sterilizing 15min, is placed in refrigerator standby.
Comparative example 4, a kind of culture medium suitable for lactobacillus acidophilus
The difference of comparative example 4 and embodiment 1 is, removes tomato juice and dandelion extract, with beer juice (Qingdao beer
Wine, 330ml/ bottles) 150.1g/L replacements, remaining parameter and operation such as embodiment 1.
The influence that test example one, different growth factors grow to lactobacillus acidophilus
Culture medium and blank control group (compared with Example 1, do not contain growth described in Example 1, comparative example 1~4
Factor tomato juice and dandelion extract), 3% lactobacillus acidophilus activating solution of each access is placed in middle training under the conditions of 37 DEG C of incubator
27h is supported, each group OD values are surveyed under 630nm from ELIASA, every group is repeated 3 times, and result of the test is as shown in table 1 and Fig. 1.
Influence (the OD that the different growth factors of table 1 grow to lactobacillus acidophilus630)
As a result show, compared with blank control group, each group has the growth of promotion lactobacillus acidophilus to a certain extent；Its
In, carrot juice growth-promoting effect is least obvious, and dandelion extract growth-promoting effect is preferable；Extracted with tomato juice or dandelion
Thing independent role is compared, and it is most notable that rush lactobacillus acidophilus growth result is applied in combination in tomato juice and dandelion extract.
The influence that experiment two, initial pH value of medium and lactobacillus acidophilus inoculum concentration grow to lactobacillus acidophilus
The influence that 2.1 initial pH value of medium grow to lactobacillus acidophilus
In order to determine the optimal initial pH value of lactobacillus acidophilus growth, the culture medium of embodiment 1 is determined respectively with ELIASA
The OD values of lactobacillus acidophilus when initial pH is 6.0,6.2,6.4,6.6,6.8, as a result as shown in Figure 2.
As can be seen from Figure 2, different initial pH values generates influence on lactobacillus acidophilus, and too low or too high initial pH value is all
It is unfavorable for the growth of lactobacillus acidophilus.When pH value is 6.2, the biology length of lactobacillus acidophilus reaches highest.Therefore, the newborn bar of acidophilus
The initial pH value of medium of bacterium is chosen to be 6.2 respectively.
The influence that 2.2 inoculum concentrations grow to lactobacillus acidophilus
Respectively by 1%, 2%, 3%, 4%, 5%, the 6% inoculum concentration access culture medium of embodiment 1, the newborn bar of acidophilus is investigated
The influence that the inoculum concentration of bacterium grows to it, as a result as shown in Figure 3.
From the figure 3, it may be seen that when inoculum concentration is 2%~4%, the fast-growth of lactic acid bacteria shortens culture to highest biomass
Cycle；When inoculum concentration is 3%, the biomass of lactobacillus acidophilus reaches maximum；When inoculum concentration is 6%, lactic acid bacteria bacterium number has
It is decreased obviously, illustrating that inoculum concentration is excessive causes lactobacter growth later stage nutritional deficiency, causes part thalline dead, bacterium number is made on the contrary
Reduction, therefore, the optimum inoculation amount of lactobacillus acidophilus for 3%.
Influence of the culture medium to lactobacillus acidophilus growth curve before and after test example three, optimization
By 3% lactobacillus acidophilus be inoculated into respectively fluid nutrient medium containing 200mL (compare with embodiment 1, do not contain tomato juice and
Dandelion extract) and the conical flask of the culture medium of 200mL embodiments 1 in, put 37 DEG C of incubator cultivate 0 respectively, 3,6,9,12,
15th, 18,21,24,27,30,33,36, after 48h, 200 μ L are taken respectively, are measured from ELIASA 630nm, with incubation time
For abscissa, absorbance is ordinate, obtains growth curve Fig. 4 of lactobacillus acidophilus before and after medium optimization.
Fig. 4 is shown, with after culture medium (not containing tomato juice and dandelion extract) inoculating lactobacillus acidophilus before optimization 0
~6h, lactobacillus acidophilus is in growth lag phase in this period, and 6~24h lactobacillus acidophilus is in logarithmic phase, and 24~33h enters
Enter stationary phase, hereafter thalline initially enters decline phase；But the culture medium after optimization (adds tomato juice and dandelion is extracted
Thing) growth of lactobacillus acidophilus is more conducive to, lactobacillus acidophilus is initially entering logarithmic phase about 12h mistakes after about 3h deadtime
Afterwards, thalline enters stationary phase, and in 21h, absorbance is maximum, significantly shorten the time into stationary phase.
The above-described embodiments merely illustrate the principles and effects of the present invention, not for the limitation present invention.It is any ripe
Know the personage of this technology all can carry out modifications and changes under the spirit and scope without prejudice to the present invention to above-described embodiment.Cause
This, those of ordinary skill in the art is complete without departing from disclosed spirit and institute under technological thought such as
Into all equivalent modifications or change, should by the present invention claim be covered.
Claims (10)
1. a kind of culture medium suitable for lactobacillus acidophilus, it is characterised in that be made up of the composition of following weight：Distilled water 1000
~2000ml, 0.2~1.0g/L of magnesium sulfate, 0.1~0.5g/L of manganese sulfate, 10~40g/L of yeast extract, dipotassium hydrogen phosphate
1.0~4.0g/L, 1.0~4.0g/L of diammonium hydrogen citrate, 5~20g/L of beef extract, 10~20g/L of agar, peptone 5~
20g/L, 0.5~2g/L of Tween 80,50~200g/L of tomato juice, 7.5~30g/L of sodium acetate, 10~40g/L of glucose and Pu
0.04~0.2g/L of public English extract.
2. it is applied to the culture medium of lactobacillus acidophilus as claimed in claim 1, it is characterised in that by following weight into packet
Into：Distilled water 1000ml, magnesium sulfate 0.58g/L, manganese sulfate 0.25g/L, yeast extract 20g/L, dipotassium hydrogen phosphate 2g/L, lemon
Lemon acid hydrogen two ammonium 2g/L, beef extract 10g/L, agar 14g/L, peptone 10g/L, Tween 80 1g/L, tomato juice 150g/L, acetic acid
Sodium 15g/L, glucose 20g/L and dandelion extract 0.1g/L.
3. as claimed in claim 1 or 2 be applied to lactobacillus acidophilus culture medium, it is characterised in that the tomato juice by with
Lower step is made：
Fresh tomato is taken, purified water warm water 5~15min of blanching of 1~2 times of amount of tomato is added after clean, section, is squeezed the juice, is filtered,
It is 6.3,110~120 DEG C of 10~15min of sterilizing to adjust pH, is produced.
4. it is applied to the culture medium of lactobacillus acidophilus as claimed in claim 1 or 2, it is characterised in that the dandelion is extracted
Thing is made by following steps：
Dandelion medicinal material is weighed, is crushed, the purified water of 10~20 times of amounts of medicinal material is added, decocted 1~3 time, 0.5~1h, is filtered every time
Cross, merging filtrate, be concentrated and dried, produced after crushing.
5. it is applied to the culture medium of lactobacillus acidophilus as claimed in claim 1 or 2, it is characterised in that at the beginning of the culture medium
Beginning pH is 6.0~6.6.
6. it is applied to the culture medium of lactobacillus acidophilus as claimed in claim 1 or 2, it is characterised in that at the beginning of the culture medium
Beginning temperature is 28~40 DEG C.
7. it is applied to the culture medium of lactobacillus acidophilus as claimed in claim 1 or 2, it is characterised in that the lactobacillus acidophilus
Inoculum concentration be 2~4%.
8. it is applied to the culture medium of lactobacillus acidophilus as claimed in claim 1 or 2, it is characterised in that the lactobacillus acidophilus
For lactobacillus acidophilus JCM1132, its deposit number is：CGMCC 1.1878.
9. a kind of method of the culture medium for lactobacillus acidophilus prepared as described in claim 1~8 is any, its feature exists
In comprising the following steps：
A) the preparation of tomato juice：Fresh tomato is taken, the purified water warm water blanching 5 that 1~2 times of addition tomato is measured after clean, section~
15min, squeezes the juice, filtering, and regulation pH is 6.3,110~120 DEG C of 10~15min of sterilizing, is placed in refrigerator preservation, standby；
B) the preparation of dandelion extract：Dandelion medicinal material is weighed, is crushed, the purified water of 10~20 times of amounts of medicinal material is added, 1 is decocted
~3 times, 0.5~1h, filtration, merging filtrate, are concentrated and dried, are produced after crushing every time；
C each component) is weighed in proportion, is mixed, pH is adjusted, and high pressure moist heat sterilization is produced.
10. a kind of lactobacillus acidophilus cultural method, it is characterised in that the strain of lactobacillus acidophilus of activation is pressed 2~4% inoculation
In the culture medium that amount accesses the culture medium as described in claim 1~8 is any or such as claim 9 is prepared, 37 DEG C are placed in
20~30h of culture in constant incubator.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107828693A (en) * | 2017-11-29 | 2018-03-23 | 江苏恒顺醋业股份有限公司 | The lactobacter growth metabolism culture medium and its application method of a kind of improvement |
| CN110106133A (en) * | 2019-05-23 | 2019-08-09 | 北京大学 | Adjust the culture solution and adjusting method of growth of lactobacillus speed and bacterium interior element content |
| CN111926053A (en) * | 2020-08-07 | 2020-11-13 | 辽宁傲农饲料有限公司 | Culture medium for counting lactic acid bacteria and saccharomycetes in compound bacteria fermented feed and preparation method and application thereof |
| CN112961797A (en) * | 2021-02-01 | 2021-06-15 | 武汉微康益生菌研究院有限公司 | Lactobacillus acidophilus high-density fermentation medium and application thereof |
| CN115820515A (en) * | 2023-01-03 | 2023-03-21 | 东北农业大学 | Preparation of cheap lactobacillus PTG medium and application of cheap lactobacillus PTG medium in pickled vegetable fermentation |
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| CN102232998A (en) * | 2011-04-15 | 2011-11-09 | 北京康华远景科技有限公司 | Micro-ecological traditional Chinese medicine preparation for enhancing immunity of livestock and poultry, and preparation method thereof |
| CN103255093A (en) * | 2013-05-20 | 2013-08-21 | 厦门六维生物科技有限公司 | Preparation method of lactobacillus acidophilus |
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- 2017-06-27 CN CN201710499446.7A patent/CN107217020B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102232998A (en) * | 2011-04-15 | 2011-11-09 | 北京康华远景科技有限公司 | Micro-ecological traditional Chinese medicine preparation for enhancing immunity of livestock and poultry, and preparation method thereof |
| CN103255093A (en) * | 2013-05-20 | 2013-08-21 | 厦门六维生物科技有限公司 | Preparation method of lactobacillus acidophilus |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107828693A (en) * | 2017-11-29 | 2018-03-23 | 江苏恒顺醋业股份有限公司 | The lactobacter growth metabolism culture medium and its application method of a kind of improvement |
| CN110106133A (en) * | 2019-05-23 | 2019-08-09 | 北京大学 | Adjust the culture solution and adjusting method of growth of lactobacillus speed and bacterium interior element content |
| CN111926053A (en) * | 2020-08-07 | 2020-11-13 | 辽宁傲农饲料有限公司 | Culture medium for counting lactic acid bacteria and saccharomycetes in compound bacteria fermented feed and preparation method and application thereof |
| CN112961797A (en) * | 2021-02-01 | 2021-06-15 | 武汉微康益生菌研究院有限公司 | Lactobacillus acidophilus high-density fermentation medium and application thereof |
| CN115820515A (en) * | 2023-01-03 | 2023-03-21 | 东北农业大学 | Preparation of cheap lactobacillus PTG medium and application of cheap lactobacillus PTG medium in pickled vegetable fermentation |
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| CN107217020B (en) | 2020-05-08 |
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Inventor after: Guan Shuyu Inventor after: Huang Yinru Inventor after: Xu Tianning Inventor after: Xu Shuochao Inventor after: Li Yang Inventor after: Liang Shengwang Inventor after: Wang Shumei Inventor before: Guan Shuyu Inventor before: Xu Shuochao Inventor before: Li Yang Inventor before: Liang Shengwang Inventor before: Wang Shumei |