CN104725129A - Method for preparing tremella fuciformis cultivation material - Google Patents

Abstract

The invention relates to a method for preparing a tremella fuciformis cultivation material and belongs to technologies for applying lentinus edodes stem feet to tremella fuciformis cultivation. Compared with the prior art, the method has the advantages that a lentinus edodes stem foot leachate is a purely-natural nutrient solution, contains carbohydrate substances, amino acids, peptides, nucleic acids and microelements and further contains abundant varieties of biological hormones, lentinus edodes stem residues also contain a large number of non-leached bio-active substances, and a lentinus edodes stem foot soaked material and cultivating raw materials are blended and are subjected to stacked fermentation, so that the propagation of beneficial microbes is promoted, microbial flora in the material is improved, and the catalytic decomposition of the cultivation material is accelerated; the fermented cultivation material has high-activity beneficial microbes and active metabolite thereof and has a promoting effect on the growth of tremella fuciformis hyphae and hypoxylon hyphae, which behave as vigorous hypha growth vigor and strong resistance to infectious microbes; resources, such as labor and coal, are saved, and environmental pollution is reduced, so that the method accords with a low-carbon and environment-friendly idea.

Description

A kind of making method of cultivating white fungus material

Technical field

The making method of a kind of cultivating white fungus material of the present invention, is the technology be applied to by mushroom tang in cultivating white fungus, belongs to fungus growing technique field.

Background technology

White fungus has another name called Tremella, tremella, is the food medicine dual-purpose bacterium that China has long enjoyed a good reputation.Record according to Compendium of Material Medica, white fungus slightly sweet flavor, cool in nature, moistening lung enriching yin, reduces phlegm, and promotes the production of body fluid, cough-relieving blood, controls dry consumptive lung disease, convulsive seizure due to phlegm-fire coughs inverse, constipation due to dry stool, and display white fungus has splendid health functions.

The traditional cultivating method of white fungus is grog cultivation, and planting material carries out inoculation culture after high pressure or normal-pressure sterilization, and the contour molecular organic material of the Mierocrystalline cellulose now in material is degraded, miscellaneous bacteria in material is also killed, therefore, when planting material sends out bacterium, mycelial growth is fast, and living contaminants is few.But along with the significantly rise of the price of labour power and fuel price, grog cultivation cost increases severely, and high-temperature sterilization also causes the loss of planting material nutritive substance, causes biological transformation ratio to reduce simultaneously.

Mushroom is famous and precious edible one of the medicinal fungus of holding concurrently in the world, and current China mushroom production accounts for 70% of world's mushroom ultimate production, has the equal mushroom culture in the province of more than 70% in China.Because champignon stems stiffness of foot in children, content of cellulose are higher, palatability is poor, and therefore, mushroom is being eaten raw or in the course of processing, the tang of mushroom can remove as tankage by the producer, causes the mushroom tang accounting for mushroom weight about 30% to become waste.

Summary of the invention

For the deficiencies in the prior art, the present invention's mushroom tang soaks expects to mix and stir to carry out banking up with culturing raw material to ferment, and do not add sterilant, sterilant during windrow, the planting material after fermentation directly packs inoculation, bacterium bag living contaminants is few, and the white fungus quality of producing is good, output is high.

The technical solution adopted in the present invention is:

A making method for cultivating white fungus material, comprises batching, fermentation, turning, pack, inoculation, sends out bacterium, concrete operation step:

(1) prepare burden: wood chip 56-64%, cotton seed hulls 14-20%, soya-bean cake 14-16%, mushroom tang 4-5%, lime 2-3%, gypsum 1%, be all the quality of dry-matter, proportioning sum is 100%; Described wood chip is the deciduous tree weed tree sawdust of particle diameter 3mm-5mm; Described mushroom tang is the tankage in the Lentnus edodes course of processing, is ground into the particle of 2mm-3mm after drying;

(2) ferment: mushroom tang particle is added clear water by 20-30 weight ratio doubly, soak 18h-24h at normal temperatures, then leach liquor is admixed in wood chip, cotton seed hulls, soya-bean cake compound together with champignon stems slag uniformly, stockpile is become high 1.0m-1.2m, wide 2m-3m, the trapezoidal heap that length is not limit, the culture material often piled controls at dry weight 300kg-500kg, after carrying out heap, surrounding is patted, on heap, insert some vertical pores with the wooden stick of diameter 10cm-15cm again, at the bottom of straight through reactor, stomatal frequency is 1/m ³, finally cover straw mat by its spontaneous fermentation;

(3) turning: after material temperature reaches 60 DEG C, keeps 24h, then carries out turning, and turning every day later once, add lime during first time turning, add gypsum during second time turning, altogether turning 3-4 time, time remaining 5d-6d, when stopping fermentation, the water content of planting material is 63%-65%;

(4) pack, inoculate, send out bacterium: the planting material fermented is after heat radiation, adopt the polyethylene cylinder sacked material of folding length 15cm, length 60cm, thick 0.04mm, when filling with substance puts into bacterial classification at two ends of bag, access 3 cave bacterial classifications respectively in the both sides of bag after charging, after inoculation, cultivating bag is emitted in dark bacteria room, temperature controls at 18 DEG C-20 DEG C, humid control is at 60%-75%, through the cultivation of 34d-36d, mycelia covers with pocket, namely enters out syrinx reason.

Mushroom tang is applied to the method for cultivating white fungus by the present invention, and apart from outside explanation, other operation steps is all undertaken by this area routine techniques.

The present invention's advantage is compared with prior art:

1. mushroom tang leach liquor is full-natural nutritive liquid, containing glucide, amino acid, peptide class, nucleic acid and trace element, bio-hormone also containing abundant species, also the biologically active substance do not leached in a large number is had in champignon stems slag, soak with mushroom tang and expect to mix and stir to carry out banking up with culturing raw material to ferment, promote beneficial microorganism breeding, improve the microorganism species in material, accelerate the catalytic decomposition of planting material;

2. the planting material after fermentation has high reactivity probiotics and active metabolite thereof, has accelerating effect, show as prosperous, the anti-miscellaneous bacteria ability of mycelium growth vigor strong to the growth of white fungus mycelia and incense ashes mycelia;

3. save the resource such as manpower, coal, decrease environmental pollution, meet low-carbon environment-friendly theory.

Embodiment

Below in conjunction with embodiment, the invention will be further described, and what do not illustrate in detail in embodiment is all state of the art.

Embodiment 1: a kind of making method of cultivating white fungus material, comprises batching, fermentation, turning, pack, inoculation, sends out bacterium, concrete operation step:

1. prepare burden: wood chip 60%, cotton seed hulls 17%, soya-bean cake 15%, mushroom tang 4.5%, lime 2.5%, gypsum 1%, be all the quality of dry-matter, proportioning sum is 100%;

2. ferment: mushroom tang particle is added clear water by 20-30 weight ratio doubly, soak 18h-24h at normal temperatures, then leach liquor is admixed in wood chip, cotton seed hulls, soya-bean cake compound together with champignon stems slag uniformly, stockpile is become high 1.0m-1.2m, wide 2m-3m, the trapezoidal heap that length is not limit, the culture material often piled controls at dry weight 300kg-500kg, after carrying out heap, surrounding is patted, on heap, insert some vertical pores with the wooden stick of diameter 10cm-15cm again, at the bottom of straight through reactor, stomatal frequency is 1/m ³, finally cover straw mat by its spontaneous fermentation;

3. turning: after material temperature reaches 60 DEG C, keeps 24h, then carries out turning, and turning every day later once, add lime during first time turning, add gypsum during second time turning, altogether turning 3 times, time remaining 5d, when stopping fermentation, the water content of planting material is 63%-65%;

4. pack, inoculate, send out bacterium: the planting material fermented is after heat radiation, adopt the polyethylene cylinder sacked material of folding length 15cm, length 60cm, thick 0.04mm, when filling with substance puts into bacterial classification at two ends of bag, access 3 cave bacterial classifications respectively in the both sides of bag after charging, after inoculation, cultivating bag is emitted in dark bacteria room, temperature controls at 18 DEG C-20 DEG C, humid control is at 60%-75%, through the cultivation of 35d, mycelia covers with pocket, namely enters out syrinx reason.

Embodiment 2: a kind of making method of cultivating white fungus material, comprises batching, fermentation, turning, pack, inoculation, sends out bacterium, concrete operation step:

1. prepare burden: wood chip 60%, cotton seed hulls 17%, soya-bean cake 15%, mushroom tang 4.5%, lime 2.5%, gypsum 1%, be all the quality of dry-matter, proportioning sum is 100%;

2. ferment: mushroom tang particle is added clear water by 20-30 weight ratio doubly, soak 18h-24h at normal temperatures, then the leach liquor after removing slag is admixed in wood chip, cotton seed hulls, soya-bean cake compound uniformly, stockpile is become high 1.0m-1.2m, wide 2m-3m, the trapezoidal heap that length is not limit, the culture material often piled controls at dry weight 300kg-500kg, after carrying out heap, surrounding is patted, on heap, insert some vertical pores with the wooden stick of diameter 10cm-15cm again, at the bottom of straight through reactor, stomatal frequency is 1/m ³, finally cover straw mat by its spontaneous fermentation;

3. turning: after material temperature reaches 60 DEG C, keeps 24h, then carries out turning, and turning every day later once, add lime during first time turning, add gypsum during second time turning, altogether turning 3 times, time remaining 5d, when stopping fermentation, the water content of planting material is 63%-65%;

4. pack, inoculate, send out bacterium: the planting material fermented is after heat radiation, adopt the polyethylene cylinder sacked material of folding length 15cm, length 60cm, thick 0.04mm, when filling with substance puts into bacterial classification at two ends of bag, access 3 cave bacterial classifications respectively in the both sides of bag after charging, after inoculation, cultivating bag is emitted in dark bacteria room, temperature controls at 18 DEG C-20 DEG C, humid control is at 60%-75%, through the cultivation of 37d, mycelia covers with pocket, namely enters out syrinx reason.

Control group: white fungus grog is cultivated, concrete operation step:

1. prepare burden: wood chip 64%, cotton seed hulls 18%, soya-bean cake 16%, lime 1%, gypsum 1%, be all the quality of dry-matter, proportioning sum is 100%;

2. spice: first wood chip, cotton seed hulls, soya-bean cake, lime, gypsum siccative are mixed thoroughly, then adding water stirs, makes the water content of planting material remain on 63%-65%;

3. pack, sterilizing: employing specification is the high-pressure polypropylene plastics bag charging of 15cm × 60cm × 0.005cm, carries out normal-pressure sterilization after pack, when kettle temperature reaches 100 DEG C, keeps 10h-12h;

4. cool, inoculate: naturally cool to less than 25 DEG C and access bacterial classification, then cultivating bag is emitted in dark bacteria room, temperature controls at 20 DEG C-22 DEG C, and humid control is at 60%-75%, and through the cultivation of 38d, mycelia covers with pocket, enters out syrinx reason.

Table one white fungus biological efficiency compares

As can be seen from Table I: the ferment effect utilizing mushroom tang leach liquor to add than single leach liquor together with the ferment effect that champignon stems slag adds is good, mycelial growth rate is fast, and bacterium bag pollution rate is low, and biological efficiency is high; Compared with control group, embodiment 1 all has obvious advantage in mycelial growth rate, anti-living contaminants and output.

Claims (1)

1. a making method for cultivating white fungus material, comprises batching, fermentation, turning, pack, inoculation, sends out bacterium, it is characterized in that:

(1) prepare burden: wood chip 56-64%, cotton seed hulls 14-20%, soya-bean cake 14-16%, mushroom tang 4-5%, lime 2-3%, gypsum 1%, be all the quality of dry-matter, proportioning sum is 100%; Described wood chip is the deciduous tree weed tree sawdust of particle diameter 3mm-5mm; Described mushroom tang is the tankage in the Lentnus edodes course of processing, is ground into the particle of 2mm-3mm after drying;

(2) ferment: mushroom tang particle is added clear water by 20-30 weight ratio doubly, soak 18h-24h at normal temperatures, then leach liquor is admixed in wood chip, cotton seed hulls, soya-bean cake compound together with champignon stems slag uniformly, stockpile is become high 1.0m-1.2m, wide 2m-3m, the trapezoidal heap that length is not limit, the culture material often piled controls at dry weight 300kg-500kg, after carrying out heap, surrounding is patted, on heap, insert some vertical pores with the wooden stick of diameter 10cm-15cm again, at the bottom of straight through reactor, stomatal frequency is 1/m ³, finally cover straw mat by its spontaneous fermentation;

(3) turning: after material temperature reaches 60 DEG C, keeps 24h, then carries out turning, and turning every day later once, add lime during first time turning, add gypsum during second time turning, altogether turning 3-4 time, time remaining 5d-6d, when stopping fermentation, the water content of planting material is 63%-65%;

(4) pack, inoculate, send out bacterium: the planting material fermented is after heat radiation, adopt the polyethylene cylinder sacked material of folding length 15cm, length 60cm, thick 0.04mm, when filling with substance puts into bacterial classification at two ends of bag, access 3 cave bacterial classifications respectively in the both sides of bag after charging, after inoculation, cultivating bag is emitted in dark bacteria room, temperature controls at 18 DEG C-20 DEG C, humid control is at 60%-75%, through the cultivation of 34d-36d, mycelia covers with pocket, namely enters out syrinx reason.