CN104721237A - Lactobacillus microecological preparation used in intestinal tract disease treatment drugs - Google Patents
Lactobacillus microecological preparation used in intestinal tract disease treatment drugs Download PDFInfo
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- CN104721237A CN104721237A CN201310714553.9A CN201310714553A CN104721237A CN 104721237 A CN104721237 A CN 104721237A CN 201310714553 A CN201310714553 A CN 201310714553A CN 104721237 A CN104721237 A CN 104721237A
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Abstract
The invention discloses a lactobacillus microecological preparation used in intestinal tract disease treatment drugs. The lactobacillus microecological preparation comprises, by mass, 15-55 parts of inactivated lactobacillus cells and lactobacillus fermentation products, wherein an inactivated lactobacillus cell concentration is IO8-IO9 per gram, and 32-48 parts of an excipient. The used lactobacilli comprises one or more of lactobacillus acidophilus, lactobacillus lactis, streptococcus thermophilus, pediococcus pentosaceus, pediococcus acidilactici, lactobacillus bulgaricus and bifidobacterium. The excipient is a mixture of corncob powder of 60-100 meshes and precipitated silica, a mixture of corn starch and precipitated silica, or a mixture of corncob powder, corn starch and precipitated silica. The lactobacillus microecological preparation effectively and economically solves the problems of live bacterium microecological preparations.
Description
Technical field:
The present invention relates to people to use or feeding micro-ecological preparation field, particularly relate to a kind of for the lactobacillus micro-ecological preparation in intestinal tract disease medicine.
Technical background:
Scale, the developing rapidly of high density poultry and aquaculture, make breeding ecological environment suffer serious impact, cause the disease that infectiousness is high, lethal is strong to take place frequently, use traditional disinfectant and antibiotic can not effectively prevent disease from occurring.Along with people improve food safety and personal health attention rate, recognize Long Term Contact antibiotic or in feedstuff, added all drawbacks that Antibiotic Additive brings: (I) life-time service antibiotic has caused the appearance of drug-fast strain; (2) cause people or poultry, Aquatic product human body immune function declines.After antibiotic is taken in by body, can be distributed to each immuning tissue's organ along with blood circulation, animal immune ability can progressively weaken.Antibiotic also can cause antigenic quality to reduce, and can cause bad impact to the inoculation of vaccine; (3) people or poultry, Aquatic product autogenous infection and superinfection may be caused.
Microbial ecological agent is that a kind of useful microorganism by living is made, it improves humans and animals intestinal microecology balance, the infection of prevention pathogenic entero becteria, improving the performances such as the immunity of humans and animals and obtained proof and accreditation fully, is a kind of Substitutes For Antibiotic of great potential.Current microbial ecological agent has been widely used in prevention and the auxiliary treatment of human or animal's intestinal tract disease.
The mechanism of action of microbial ecological agent mainly comprises: (I) competitiveness adheres to.By at intestinal bound site inhibition effect, form biological protection film, protection humans and animals is from the infringement of pathogenic microorganism.(2) antimicrobial effect.The lactic acid that lactic acid bacteria fermentation produces reduces intestinal PH, stops growth of pathogenic bacteria, produces bacterioid element and can kill pathogenic bacterium.(3) activate immunity function.Microbial ecological agent bacterial strain can affect host's natural immunity, humoral and cellular immune response system, improves intestinal antibody horizontal and macrophage activity, enhancing human body immunity function; (4) improve digestion power, improve food or feed digestion level.Useful microorganism, at some enzyme materials of human or animal's intestinal secretion, can promote the decomposition of food or feedstuff Middle nutrition composition, more be conducive to human or animal's digesting and assimilating nutrition; Microbial ecological agent bacterial strain can stimulate the growth of intestinal villi, increases alimentation area.
Although effect of microbial ecological agent is extensively proved and is accepted, but, still there is following problem in this kind of viable bacteria body microbial ecological agent: (I) by the impact of environmental condition (temperature, moisture, acid-base value etc.), its active ingredient easy in inactivation, thus reduce result of use; (2) pyroprocess of feed preparation and pelletize makes a large amount of viable bacterias lethal; (3) that add in feedstuff or therapeutic process use antibiotic and chemosynthesis antibacterial, have equally microbial ecological agent and kill or inhibitory action; (4) effect of the hydrochloric acid or bile etc. in human or animal's digestive tract is not highly resistant to, poor at the colonization ability of intestinal.
The same active bacteria formulation of long-term use in same growing environment, causes phage-infect to increase with the risk broken out.These problems significantly limit the application of viable bacteria class microbial ecological agent.
Summary of the invention
The object of the invention is to the deficiency overcoming existing viable bacteria class microbial ecological agent, provide a kind of brand-new, stable, inactivated lactobacillus microecological agent efficiently, with the lactobacillus cell of deactivation and fermentating metabolism product thereof for principle active component.
For achieving the above object, the present invention adopts following technical scheme:
For the lactobacillus micro-ecological preparation in intestinal tract disease medicine, comprise the component of following quality proportioning: deactivation lactobacillus cell and lactic acid bacteria fermentation product 10-60 part, wherein deactivation lactobacillus cell IO8-IO9/gram, excipient 30-50 part; The lactobacillus micro-ecological preparation of described deactivation also comprises quick-acting energy matter 10-50 part; Described lactobacillus be selected from bacillus acidophilus, lactobacillus lactis, streptococcus thermophilus, Pediococcus pentosaceus, pediococcus acidilactici, Lactobacillus bulgaricus, bacillus bifidus one or more; Described excipient is the mixture of the mixture of the mixture of 60-100 order maize cob meal and precipitated silica, corn starch and precipitated silica or maize cob meal, corn starch and precipitated silica three.
Preferably, described quick-acting energy matter is glucose or lactose.
Present invention also offers the preparation method for the lactobacillus micro-ecological preparation in intestinal tract disease medicine, comprise the following steps:
I) choose lactic acid bacteria fermentation to cultivate;
2) in fermentation liquid, the protective agent that mass fraction is 0.1-0.5% is added after fermentation ends;
3) deactivation, collected by centrifugation thalline;
4) concentrated supernatant, adds excipient, dry, makes residual moisture be less than 15%;
5) by step 3) thalline and the step 4 that obtain) product mix.
Preferably, described step 4) in, quick-acting energy matter can also be added.
Preferably, described quick-acting energy matter is glucose or lactose.
Preferably, described protective agent is the mixture of sodium alginate and glycerol, or skim milk.
Preferably, the mass ratio of described sodium alginate and glycerol is I:I.
Preferably, described ablation method can be freezing deactivation, hot deactivation or chemical ablation.
Preferably, the application of inactivated lactobacillus microecological agent in the medicine for the preparation of prevention or auxiliary treatment human or animal intestinal tract disease.
The lactobacillus micro-ecological preparation of deactivation of the present invention has effect identical with the microbial ecological agent of living, although through inactivation treatment after lactic acid bacteria fermentation, the lactobacillus cell of deactivation efficiently can not only adhere to the intestinal epithelial cell of humans and animals, play protective effect, but also there is the advantage that microbial ecological agent alive do not have: the lactobacillus cell of deactivation is high temperature resistant, stomach juice-resistant and resistance to bile, can with antibiotic synergism; In addition, lactic acid bacteria fermentation process produces metabolite such as lactic acid, bacterioid element etc. can suppress or kill the pathogenic entero becteria of human or animal effectively; Inactivated lactobacillus microecological agent of the present invention solves the problem of puzzlement viable bacteria class microbial ecological agent at present effectively, economically.
Detailed description of the invention
Embodiment 1
For the lactobacillus micro-ecological preparation in intestinal tract disease medicine, its component is that bacillus acidophilus's microbial ecological agent of per kilogram deactivation contains: the bacillus acidophilus of deactivation and tunning 550 grams thereof, wherein the lactobacillus cell content of deactivation is IO8/g, specification is 80 object maize cob meals and precipitated silica totally 450 grams, wherein maize cob meal and each 225 grams of precipitated silica.Consumption in one ton of complete feedstuff is 2 kilograms.This component ratio and addition may be used for culture fishery.
Embodiment 2
Bacillus acidophilus's microbial ecological agent preparation method of deactivation described in example I, comprises the steps:
1) bacillus acidophilus first carries out strain preparation, and bacterial screening culture medium is using solid MRS culture medium as minimal medium, adds 0.1%CaCO3, and pH value is 6.4.121 DEG C of sterilizing 20-30 minute, are cooled to 37 DEG C, for subsequent use.
2) fermentation culture of bacillus acidophilus, fermentation medium is using liquid MRS culture medium as minimal medium, and pH value is 6.4.121 DEG C of sterilizing 20-30 minute, are cooled to 37 DEG C, for subsequent use.Seed cell age 12-18 hour, inoculum concentration is 3%-5% (V/V or V/W).Fermentation time 36-48 hour, fermentation temperature 37 DEG C, pH value maintains about 5.5.
3) will add the protective agent of mass fraction 0.1-0.5% (w/v) in fermentation liquid after fermentation ends, described protective agent is made up of sodium alginate and glycerol, and the ratio of the two is I:I.
4) deactivation: freezing deactivation, centrifugal 15 minutes of fermentation liquid 3000 revs/min, removes supernatant, collects thalline and is cooled to about 10 DEG C, maintains 2-5 hour.
5) supernatant is through economic benefits and social benefits vacuum concentration, concentrated 10 times, the bacillus acidophilus of 550 grams of deactivations and tunning thereof add excipient 450 grams, described excipient is the mixture of 80 order corn starchs and precipitated silica, the two ratio is I:1, mix and stir 20-30 minute, making it fully mixing and absorption.
6) with the bacillus acidophilus of the well-mixed deactivation of excipient and tunning thereof in 90 DEG C _ 105 DEG C dryings, make residual moisture <15%.
The protective agent be made up of sodium alginate and glycerol; can the form of Cell protection, the integrity of Cell protection surface group, ensures that it can be combined with intestinal site; if do not add protective agent can cause more than 80% cell rupture before deactivation, add this protective agent and can ensure that more than 95% cell is complete.In addition, also can with skim milk as protective agent.
Containing the enzyme such as amylase, Lactose enzyme in bacillus acidophilus's tunning, contribute to animal digestion; Bacterioid element, has and suppresses pathogenic bacteria effect; The organic acid such as lactic acid, acetic acid.
Embodiment 3
The preparation method of the Lactobacillus bulgaricus microbial ecological agent of deactivation described in embodiment 3, comprises the steps:
I) Lactobacillus bulgaricus first carries out strain preparation, and bacterial screening culture medium is using solid MRS culture medium as minimal medium, adds 0.1%CaCO3, and pH value is 6.4.121 DEG C of sterilizing 20-30 minute, are cooled to 37 DEG C, for subsequent use.
2) fermentation culture of Lactobacillus bulgaricus, fermentation medium is using liquid MRS culture medium as minimal medium, and pH value is 6.4.121 DEG C of sterilizing 20-30 minute, are cooled to 37 DEG C, for subsequent use.Seed cell age 12-18 hour, inoculum concentration is 3%-5% (V/V or V/W).Fermentation time 36-48 hour, fermentation temperature 37 DEG C, pH value maintains about 5.5.
3) will add the protective agent of mass fraction 0.1-0.5% (w/v) in fermentation liquid after fermentation ends, described protective agent is skim milk.
4) deactivation: hot deactivation, fermentation liquid is warming up to 70-80 DEG C, and maintain 30_60 minute, centrifugal 15 minutes of fermentation liquid 3000 revs/min, removes supernatant, collects thalline.[0048] 5) supernatant is through economic benefits and social benefits vacuum concentration, concentrated 10 times, the Lactobacillus bulgaricus of 600 grams of deactivations and the concentrate of tunning thereof add excipient 300 grams and glucose 100 grams, described excipient is the mixture of 100 order maize cob meals, corn starch and precipitated silica, proportions is I:I:I, mix and stir 20-30 minute, making it fully mixing and absorption.[0049] 6) with the Lactobacillus bulgaricus of excipient and the well-mixed deactivation of glucose and tunning thereof in 900C_105 DEG C of drying, make residual moisture <15%.
Described ablation method can also be chemical ablation, such as, add formalin-inactivated.
Described glucose or lactose, can stress with under state of necessitys as quick-acting energy matter, and Fast back-projection algorithm ATP, provides energy to body.
Embodiment 4
For the lactobacillus micro-ecological preparation in intestinal tract disease medicine, its each component is that the lactobacillus lactis microbial ecological agent of per kilogram deactivation contains: the lactobacillus lactis of deactivation and tunning 400 grams thereof, wherein the lactobacillus lactis cell content of deactivation is ^IO8/g, lactose 300 grams, specification is 100 object corn starchs and precipitated silica totally 300 grams, wherein maize cob meal 200 grams, precipitated silica 100 grams.I.5 kilogram be at the consumption of one ton of complete feedstuff, this component ratio and addition may be used for poultry farming, in the test of bamboo silk chicken, this microbial ecological agent that with the addition of 0.15% can improve bamboo silk chicken from birth to the average production performance of 90 ages in days, the feedstuff-meat ratio of test group is 3.37, survival rate is 93.04%, and matched group is 3.60 and 91.77%.
Embodiment 5
A kind of bacillus bifidus microbial ecological agent of deactivation, its each component is that the bacillus bifidus microbial ecological agent of per kilogram deactivation contains: the bacillus bifidus of deactivation and tunning 200 grams thereof, wherein the lactobacillus cell content of deactivation is IO8/g, glucose 500 grams, specification is 80 object precipitated silica 300 grams.It can be applied to aquaculture.In the feedstuff of Paralichthys olivaceus, add the deactivation microbial ecological agent of 0.I%, find after feeding one week, test group there is no the intestinal tract diseases such as enteritis occurs, mortality rate 0.5%; Blank group and added with antibiotic group there occurs serious enteritis, and mortality rate reaches 4%.
Embodiment 6
Bacillus acidophilus's microbial ecological agent of deactivation described in example I is fed piglet experiment
Experimental period: 46 days.
Test combinations: be divided into three groups of feedstuffs, is set to A, B, C material.A material contrasts materials for (based on 311 material daily ration), the bacillus acidophilus microbial ecological agent of B material for adding deactivation described in lkg/t example I on basal diet, C material is for adding bacillus acidophilus's microbial ecological agent of deactivation described in 2kg/t example I at basal diet.
Subjects: eutocous 18 sow litters (average 10/nest) are experiment piglet, totally three batches, count 54 sows and produce 540 piglets.According to the childbirth situation of sow, determine the experiment piglet number often criticized and often organize.
EXPERIMENTAL DESIGN: be divided into three test group, is respectively matched group, test I group and test II group, as following table one.
Grouping tested by table one
Successively be divided into one, two, three batch experiment according to sow expected date of confinement order, in every batch, be also successively divided into A, B, C totally 6 repeated trials, as table two according to expected date of confinement order.
The test materials sequence of each batch, table two
" pig farm feeding and management handbook " that daily feeding and management is formulated by company of raising pigs carries out, free choice feeding, fully drinks water, and conventional epidemic prevention, often sterilizes.Every day, piglet was fed in raw material four times, and every day feeds by standard weighing, and the next morning reclaims residue material and weighs, and observe the dysentery situation of piglet every day, piglet searches for food and health status.
Experimental data: the record condition of production of sow, piglet Birth weight, 26 day age weaning weight, 35 day age be heavy; By day entry additive amount and residue doses, record summer dysentery head number and each stage respectively organize weight gain of piglets, analyze dysentery incidence rate.
Data Summary:
Dysentery is analyzed, and as can be seen from Table III, often in group test piglet, matched group diarrhea rate is 11.32%, and test I group is 2.25%, and I.62% test I I group is.The bacillus acidophilus's microbial ecological agent adding deactivation in feedstuff can reduce piglet diarrhea rate, and test I, II group respectively comparison duty group diarrhea rate reduces by 80.12% and 85.68%.And the test group piglet colour of skin is ruddy, good bloom, within 35 days, survival rate is to 95.4% and 95.3%, improves 1% and 0.95% respectively than matched group.
Average daily gain is analyzed, from birth to 35 ages in days, matched group average daily gain is 191 grams/day/head, and test I group and test II group are respectively 212 grams/day/and 206 grams/day/head, and test I group and the average daily gain of test II group improve 10.99% and 7.85% than matched group respectively.
Table three test data statistical table
The price of deed is analyzed, and from the 25 to the 35 day, the daily material consumption of matched group, test I group and test II group was respectively 215.57 grams/day/, 222.64 grams/day/, 234.97 grams/day/head, I.13 feedstuff-meat ratio is respectively, I.05, I.14, I group is wherein tested best.
Conclusion:
I) the bacillus acidophilus's microbial ecological agent adding deactivation in little pig feed can maintain the suitable acidity of piglets gastric to intensify the activity of enzyme, improve substance digestion rate, pathogenic bacterium also can be suppressed to breed at gastrointestinal, thus reduce diarrhoea, improve weightening finish, improve feed conversion rate.
2) in two tests, the test effect of B material (namely testing I group) is relatively good, and the performance of test piglet growth is good, and fur glow, prothymia, has no adverse reaction.
3) from Economic and Efficiency Analysis, B material (namely the testing I group) price of deed low (namely feed conversion rate is high), production cost are lower, and economic benefit is obvious.
4) after early weaning at present, baby pig diarrhoea incidence rate is higher, the drug resistance that a large amount of use antibiotic medicine makes piglet produce is more and more stronger, test proves, bacillus acidophilus's microbial ecological agent of deactivation effectively can prevent the generation of dysentery, therefore the bacillus acidophilus's microbial ecological agent adding suitable deactivation at piglets material be an effective way reducing baby pig diarrhoea the above, it is only preferred embodiment of the present invention, it is not restriction the present invention being made to other form, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the Equivalent embodiments of equivalent variations.But everyly do not depart from technical solution of the present invention content, any simple modification, equivalent variations and the remodeling done above embodiment according to technical spirit of the present invention, still belong to the protection domain of technical solution of the present invention.
Claims (2)
1. for the lactobacillus micro-ecological preparation in intestinal tract disease medicine, it is characterized in that, comprise the component of following quality proportioning: deactivation lactobacillus cell and lactic acid bacteria fermentation product 15-55 part, wherein deactivation lactobacillus cell IO8-IO9/gram, excipient 32-48 part, described lactobacillus is selected from bacillus acidophilus, lactobacillus lactis, streptococcus thermophilus, Pediococcus pentosaceus, pediococcus acidilactici, Lactobacillus bulgaricus, one or more in bacillus bifidus, described excipient is the mixture of 60-100 order maize cob meal and precipitated silica, the mixture of corn starch and precipitated silica, or maize cob meal, the mixture of corn starch and precipitated silica three.
2. according to claim I for the lactobacillus micro-ecological preparation in intestinal tract disease medicine, it is characterized in that: also comprise glucose or lactose.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107950792A (en) * | 2017-12-12 | 2018-04-24 | 生物源生物技术（深圳）股份有限公司 | A kind of New Type Complex Fead Additives and its preparation method and application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101612169A (en) * | 2008-06-27 | 2009-12-30 | 生物源生物技术（深圳）有限公司 | Lactobacillus micro-ecological preparation of deactivation and preparation method thereof |
| CN101971921A (en) * | 2010-11-12 | 2011-02-16 | 胡文锋 | Zinc-enriched inactivated lactobacillus microecological agent and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101612169A (en) * | 2008-06-27 | 2009-12-30 | 生物源生物技术（深圳）有限公司 | Lactobacillus micro-ecological preparation of deactivation and preparation method thereof |
| CN101971921A (en) * | 2010-11-12 | 2011-02-16 | 胡文锋 | Zinc-enriched inactivated lactobacillus microecological agent and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107950792A (en) * | 2017-12-12 | 2018-04-24 | 生物源生物技术（深圳）股份有限公司 | A kind of New Type Complex Fead Additives and its preparation method and application |
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Application publication date: 20150624 |