Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
This application provides a kind of mineral substance synergia compound fertilizer material, mainly comprise diammonium phosphate, humic acid powder, potassium-bearing shale powder, bacillusmusilaginosiengineering and bacillus megaterium, consisting of wherein according to weight percent: diammonium phosphate 75%-85%; Humic acid powder 5%-9%; Potassium-bearing shale powder 6%-9%; Bacillusmusilaginosiengineering 1%-4%; Bacillus megaterium 1%-4%.Wherein, bacillusmusilaginosiengineering is concentrated bacterium liquid, and viable bacteria content is 5,000,000,000/milliliter, and it is 10,000,000,000/milliliter that bacillus megaterium concentrates bacterium liquid hold-up.
In the present embodiment, the diammonium phosphate in mineral substance synergia compound fertilizer material gets 80%, and humic acid powder gets 6%, and potassium-bearing shale powder gets 8%, and bacillusmusilaginosiengineering gets 3%, bacillus megaterium 3%.Wherein, in diammonium phosphate, nitrogen element accounts for 12%, and phosphoric accounts for 16%; Content of humic acid 80% in humic acid powder; Potassium content in potassium-bearing shale powder is 12.5%; Bacillus megaterium is concentrated bacterium liquid, in product total viable count be 100,000,000/gram; Bacillusmusilaginosiengineering is also concentrated bacterium liquid, in product total viable count be 0.5 hundred million/gram.
The mineral substance synergia compound fertilizer material that the application provides is mainly crop and provides and enrich mineral element, main containing nitrogen element and phosphoric in diammonium phosphate, and nitrogen element accounts for 12%, and phosphoric accounts for 16%; Accordingly, potassium-bearing shale powder mainly provides potassium element.In order to the phosphoric in diammonium phosphate and potassium-bearing shale powder and potassium element can be discharged effectively and by farm crop efficient absorption, the application with the addition of bacillusmusilaginosiengineering and bacillus megaterium in mineral substance synergia compound fertilizer material.
Wherein, bacillusmusilaginosiengineering has potassium decomposing effect to general potassium-bearing mineral, the general character of potassium decomposing effect is: form bacterium-mineral complex body and produce bacterium thus to the corrosion of mineral surface, along with the carrying out of corrosion, mineral grain lattice deforms and disintegration gradually.Bacillusmusilaginosiengineering carries out active absorption to mineral K, and simultaneously the chemokines such as its meta-bolites carries out chemical degradation effect to mineral grain.
At the identical bacterial strain of employing with under same culture conditions, bacillusmusilaginosiengineering also has significant difference to different sorts mineral potassium decomposing effect power, and this difference is because the difference of mineral lattice structure and potassium ion existence causes.For the potassium-bearing mineral of rack-like structure, bacillusmusilaginosiengineering, mainly through destroying the crystalline network of potassium felspar sand, discharges potassium, and produces the potassium decomposing effect that the organic acids such as lactic acid, citric acid, tartrate, oxysuccinic acid realize mineral; And the potassium-bearing mineral for laminate structure, such as potassium-bearing shale, bacillusmusilaginosiengineering promotes the release of wherein potassium ion by the distance expanding its interlamination region.
In above-mentioned mechanism, this bacterium that is formed as of bacterium-mineral complex body provides very favourable place to mineral second time potassium decomposing effect, and it is the key point of bacillusmusilaginosiengineering to mineral potassium decomposing effect.The potassium ion discharged through said process exists with the form of organic-electrodeless complex body, and is directly absorbed by plant.
In addition, bacillusmusilaginosiengineering also has releases potassium characteristic, provides following experiment simultaneously:
Carried out releasing potassium approval test under shake flask culture conditions for potassium-bearing mineral with potassium felspar sand, potassium-bearing shale and illite respectively by bacillusmusilaginosiengineering, the results are shown in Figure 1, Fig. 1 is the histogram of gel-shaped series bacillus to the Decomposition of different potassium-bearing mineral.
As seen from Figure 1, different potassium-bearing minerals, because its Nomenclature Composition and Structure of Complexes is different, the ability of antibiont weathering is just different.Potassium felspar sand is a kind of primary mineral, compare and be difficult to decompose, and shale and illite is secondary mineral, are easily decomposed.After shake-flask culture 7d, bacillusmusilaginosiengineering increases by 85.8% (potassium felspar sand), 96.5% (illite) and 115.2% (potassium-bearing shale) to three kinds of ability of dissolving potassium for examination mineral respectively than the contrast of inoculation inactivated bacteria.
Therefore, in the mineral substance synergia compound fertilizer material that the application provides, add bacillusmusilaginosiengineering and potassium-bearing shale, the efficient release of potassium ion can be realized and farm crop can be made to absorb potassium ion efficiently.
The bacillus megaterium that the application provides has effect of good degrading organic phosphor, by the phosphoric degraded in diammonium phosphate, farm crop directly can be absorbed, substantially increase the utilization ratio of phosphoric.Research simultaneously shows, phosphate-solubilizing bacteria such as bacillus megaterium is made microbial inoculum and is manured into soil, and not only can increase crop yield and improve the quality of crop, and can reduce the amount of application of phosphate fertilizer, the utilising efficiency of raising phosphorus.
Meanwhile, also have the capacity of decomposition stronger to potassium-bearing shale after the bacillusmusilaginosiengineering that the application provides and bacillus megaterium mixing, bacillusmusilaginosiengineering and the mixed fermentation of bacillus megaterium shaking flask of side specific as follows are tested potassium-bearing shale capacity of decomposition.
Strains tested: potassium decomposing class bacillusmusilaginosiengineering conciliates Phosphorus bacillus megaterium.
Potassium-bearing shale (washing away water-soluble potassium with deionized water): containing K
2o is 13.2%, and silicon is 46%, and magnesium is 4%, and zinc is 4.3%, and iron is 5.2%, and molybdenum is 0.22%.
Substratum: have nitrogen potassium-bearing shale substratum, containing sucrose 10.0, (NH
4)
2sO
41.0, Na
2hPO
42.0, MgSO
47H
2o0.5, NaCl0.10, yeast extract paste 0.5, CaCO
31.0, pH7.4; Add 1% potassium-bearing shale.
Test method:
There is nitrogen potassium-bearing shale substratum, every 500ml triangular flask loads 100ml substratum, every bottle adds potassium-bearing shale powder 3.0g, 121 DEG C of sterilizing 25min, respectively by 2.5% inoculum size inoculation bacillusmusilaginosiengineering after cooling, cultivate 12 hours inoculation bacillus megateriums, contrast adds equivalent inactivated bacterial liquid and does not connect bacterium, 3 repetitions are established in test, 30 DEG C of rotary shaker (220rpm) shaking culture, incubation time is 0, 12, 24, 48, 72, 96, sample after 120h, sample is at the centrifugal 15min of 3000rpm, get supernatant liquor and measure available potassium, rapid available phosphorus, silicon, iron, manganese plasma content.
Results and analysis
Show through multiple batches of mensuration, mixed fermentation liquid shows stronger decomposition potassium-bearing shale ability under shaking flask condition, and this capacity of decomposition strengthens along with the prolongation of time.Measure display, constant temperature culture 48h, and add equivalent inactivated bacterial liquid and compare, mixed fermentation liquid only can discharge K from potassium-bearing shale
2o81.6mg/L, SiO
25.4mg/L, Fe
2o
31.5mg/L, MnO0.06mg/L, P
2o
51.1mg/L; Improve 97.1%, 31.0%, 16.3%, 60% and 40.7% respectively; Cultivate 120h, only discharge K
2o113.1mg/L, SiO
214.3mg/L, Fe
2o
37.1mg/L, MnO0.1mg/L, P
2o
52.0mg/L; Improve 232.6%, 82.2%, 73.9%, 100% and 74.1% respectively, and add equivalent inactivated bacterial liquid (CK
1) and do not meet bacterium (CK
2) two process substantially constant.Pole conspicuous level is all reached through biometrics.
The mineral substance synergia compound fertilizer material that the application provides not only has stronger potassium decomposing dissolving P capacity, also can increase soil effective K, phosphorus and silicon, iron, manganese, zinc level of supply, specifically proves this conclusion by following experiment.
Bacillusmusilaginosiengineering and bacillus megaterium bacterial strain and the composite potted plant decomposition approval test of potassium-bearing shale
Test materials
For examination soil: the yellowish soil of 126 DEG C/2h sterilizing;
For examination fertilizer: the mineral substance synergia compound fertilizer material (containing bacillusmusilaginosiengineering, bacillus megaterium and potassium-bearing shale) that the application provides
Experimental cultivar: Soviet Union green pepper No. 5
Test design
This test is potted plant soil with sterile soil, and whether when nitrogenous fertilizer, Phosphorus fertilizer usage are constant, by reducing Potassium Fertilizer, investigating can part Potassium Fertilizer after applying potassium-bearing shale.Full dose potassium is set in test, and (consumption of potassium is as K
2o0.15g/kg), 1/2 amount potassium, 1/3 amount potassium and do not add potassium 4 groups.Every group all establishes and does not connect bacterium, connect bacterium, do not connect bacterium+potassium-bearing shale, connect inactivated bacteria+potassium-bearing shale and connect bacterium+potassium-bearing shale 5 process, repeats 3 times.Every basin fills native 800g, and add the every basin of potassium-bearing shale process and to spread manuer in holes 0.5g, connecing the bacterium process amount of spreading manuer in holes is that (viable count is 5.10 × 10 to 5ml/ basin
8individual/ml), connect inactivated bacteria process and to connect bacterium process dosage identical with method.Test materials mixing dress basin, plant pepper seed, every basin final singling 1 strain, hot-house culture after 28 DEG C of cultivation 5d, every 7d waters 150ml aseptic deionized water, and after cultivating 25d, each process imposes equivalent N0.015g/kg, P simultaneously
2o
50.01g/kg, cultivates 60d post-sampling, measures the potassium on plant, the full potassium of Simultaneously test soil, available potassium, rapid available phosphorus, and silicon, iron, manganese, zinc nutrition element.
Interpretation of result
Connect bacterium+potassium-bearing shale process to be better than connecing bacterium and not add potassium-bearing shale process
As seen from Table 1, after adopting sterile soil to cultivate, connect bacterium+potassium-bearing shale process and all connect bacterium process significantly better than what do not add potassium-bearing shale.In Quan Jia group, plant height, dry weight, plant potassium improve 11.9%, 25.8% and 5.4% respectively; 13.4%, 27.6% and 12.9% is improved respectively in 1/2 group; 14.6%, 30.9% and 13.4% is improved respectively in 1/3 group; In Bu Jia potassium group, improve 16.7%, 34.0% and 34.3% respectively, and improve gradually along with the indices that successively decreases of potassium amount.
Connect bacterium process to be better than not connecing bacterium process
No matter be full potassium, 1/2 potassium, 1/3 potassium or Bu Jia potassium group connects bacterium process capsicum plant height, dry weight and plant potassium content all apparently higher than not connecing bacterium, not connecing bacterium+shale and connecing the process of 3, inactivated bacteria+shale.In Quan Jia group, plant height, dry weight, plant potassium improve 8.5%-8.9%, 18.7%-19.1% and 5.7%-6.1% respectively; 12.6%-13.0%, 20.6%-21.3% and 3.4%-4.3% is improved respectively in 1/2 group; 25.1%-26.1%, 40.8%-41.4% and 12.3%-12.8% is improved respectively in 1/3 group; 7.3%-7.8%, 11.4%-12.4% and 14.5%-15.3% is improved respectively in Bu Jia potassium group.
Connect bacterium+potassium-bearing shale 1/2,1/3 amount potassium process suitable with the process of full dose potassium
Connect the process of bacterium+potassium-bearing shale 1/2,1/3 amount potassium to compare with the process of full dose potassium, at plant height, dry weight and plant potassium content index low 0.1-0.2cm, 0.01-0.04g and 0.1-0.3mg respectively.
Table 1 sterile soil mixed bacteria liquid is on the impact of chili growth
, show through method measurement results such as atomic absorption, molybdenum blue colorimetrics, after using sterile soil plantation capsicum, the full potassium of mixed bacteria liquid 1/3 amount potassium process soil comparatively connects inactivated bacteria and does not connect bacterium and reduces by 1.33% and 1.35% respectively meanwhile; Available potassium increases by 30.58% and 30.41% respectively; Rapid available phosphorus increases by 27.80% and 28.38% respectively; Other available nutrient increases silicon respectively: 27.3% and 24.8%, iron: 16.5% and 17.8%, manganese: 53.2% and 51.3%, zinc: 33.3% and 45.5%, please refer to table 2.
Table 2 1/3 amount potassium process mixed bacteria liquid is to soil nutrient elements capacity of decomposition
More than test explanation, bacillusmusilaginosiengineering and bacillus megaterium can decompose potassium in potassium-bearing shale and other nutritive substance, the nutrition supply of plant can not only be met in time, growth promoting effects, the content of potassium element in plant body can also be improved, increase soil effective K, phosphorus and silicon, iron, manganese, zinc level of supply, and partly can replace Potassium Fertilizer after using potassium-bearing shale.Therefore, biotechnology activation potassium-bearing shale is adopted to be feasible in production.
The mineral substance synergia compound fertilizer material that the application provides also has the effect increasing crop yield, refers to following bacillusmusilaginosiengineering and bacillus megaterium and potassium-bearing shale composite to sweet persimmon green pepper effect test.
For examination soil: unmanured silty loam for many years.
For examination fertilizer: mineral substance synergia compound fertilizer material that the application provides (mainly comprises the mixed bacteria liquid of potassium-bearing shale, gel-shaped thatch spore bacillus and bacillus megaterium.)
Base manure kind: Chicken dung, Nitrogen, Phosphorus and Potassium (N, P, K are respectively containing 16%).
Experimental cultivar: sweet persimmon green pepper.
Test method: after transplanting, adopts in capsicum rhizosphere side and digs cave and cover the method for burying, and will to mix with other fertilizer and be spread in cave for trying fertilizer, earthing waters, and trial period only imposes once.Impose once at seedling-slowing stage and Sheng phase of blooming by every 667m2 urea 10kg, the agronomic measures such as to intertill, water consistent.
Yield result is analyzed
As can be seen from Table 3, use for examination soil fertility quality (T
2) and blank (CK
1), conventional fertilizer application (CK
2), conventional fertilizer application+etc. nutrient matrix (T
1) process is compared, T
2with CK
2relatively, its output all reaches pole conspicuous level; T
1with T
2relatively, close to conspicuous level.
Table 3 cell production otherness compares
LSD0.05=2.53 LSD0.01=3.83
Proved by the test of community effect, under this yield level condition, every 667m
2use mineral substance synergia compound fertilizer material that 5kg the application provides comparatively the nutrient such as conventional fertilizer application, deactivation matrix, blank increase production 12.1%, 6.0% and 27.5% respectively, through biometrics, its volume variance reaches pole conspicuous level.
The mineral substance synergia compound fertilizer material that the application provides, not only achieve the efficient-decomposition of potassium element and phosphoric to realize effectively absorbing of crop, also can realize the raising of crop disease-resistant ability, proving through related experiment, there is probability that is withered and verticillium evil and significantly reduces in the plot of using the mineral substance synergia compound fertilizer material that the application provides.Compare compared with the continuous cropping contrast of 3 years, eggplant, tomato, cucumber sickness rate reduce by 21.4%, 27.8% and 37.3% respectively; Compared with the continuous cropping matrix such as nutrient such as grade of 3 years, reduce by 21.0%, 27.3% and 37.0% respectively; Compare with the main crop, respectively high by 1.6%, 1.5% and 2.9%; Its preventive effect comparatively contrasts and reaches 87.3%, 88.3% and 88.5% respectively, comparatively waits nutrient matrix to reach 87.1%, 88.3% and 88.5% respectively.
From above-mentioned experimental result and analyze, the nutritive element that abundant Gong the farm crop that the mineral substance synergia compound fertilizer material that the application provides can provide for soil directly absorb, while can also realize the volume increase of farm crop and the raising of resistance against diseases.
The mineral substance synergia compound fertilizer material that the application provides also comprises humic acid, and humic acid has that molecular weight is little, active gene is high, improve soil, improve ground temperature, impel root system to reach, and effectively regulates soil acidity or alkalinity, solves the unique effects of the aspects such as soil compaction.Humic acid and organic or inorganic chemical fertilizer (as nitrogen, phosphorus, potassium etc.) with the use of, can fixed nitrogen, phosphorus decomposing, slowly-releasing potassium, impel effective ingredient to transform, as early as possible utilization absorbed by crops, improve various effective ingredient-use rate more than 80%.
The application of humic acid on vegetables: mainly prevent Vegetable Soil continuous cropping from polluting, continuous cropping courses of infection; Can spoiled soil germ reproduction effectively; Strengthen soil aggregate, effectively impel crop to take root, Sheng Xingen, impels well developed root system more, improves plant resistance against diseases, stimulates vegetable growth, strengthens crop photosynthesis, makes vegetable growth vigorous, increase the yield per unit area, and increases vegetable grower's income.
The application of humic acid on fruit tree: fruit tree well developed root system can be impelled
Proved by great number tested data such as the above potassium decomposing to the mineral substance synergia compound fertilizer material that the application provides, effect of solubilizing phosphate test, field crops Piglet s colibacillosis and disease-resistant yield-increasing effects, the mineral K in the mineral substance synergia compound fertilizer material that the application provides in potassium-bearing shale can be decomposed into effective potassium by bacillusmusilaginosiengineering and absorb for crop.Bacillus megaterium also can realize the decomposition having phosphoric in diammonium phosphate simultaneously, improves the utilization ratio of phosphorus in diammonium phosphate.
This application provides a kind of method preparing mineral substance synergia compound fertilizer material, as follows:
Step 1, first take the granule type diammonium phosphate of predetermined amount with electronic scale, and be fed into uniformly in drum drying equipment according to the speed of 8.5 tons/hour;
Step 2, humic acid powder is mixed with bacillus megaterium bacterium liquid proportional after, utilize electronic scale feeding and be added into equably in drum drying equipment according to the speed of 0.5 ton/hour, mixing with graininess diammonium phosphate;
Step 3, potassium-bearing shale powder is mixed with bacillusmusilaginosiengineering bacterium liquid proportional after, utilize electronic scale feeding and be added into equably in drum drying equipment according to the speed of 0.8 ton/hour, mixing with bacillus megaterium with graininess diammonium phosphate, humic acid powder;
While the above material of interpolation, utilize volume pump by water with 200 kilograms, jet pipe mist water, overlay film solvent 30 kgs/hour, and ingress's hot blast temperature of index drum drying plant is 150 degrees Celsius, ingress's temperature of charge is 50-60 degree Celsius.
Through oven dry and the aluminum coated steel of drum drying equipment, diammonium phosphate particle attached to the humic acid powder, potassium-bearing shale powder, bacillus megaterium bacterium liquid and the bacillusmusilaginosiengineering bacterium liquid that add in proportion, define the mineral substance synergia compound fertilizer material that final the application provides, finally by the material sub-sieve of mineral substance synergia compound fertilizer and finally pack.
It should be noted that, control at 50-60 degree Celsius at the exit hot blast temperature of drum drying equipment, temperature of charge is 30-35 degree.
This production technique, without the need to through traditional roasting or chemical industry process, has and produces the advantages such as easy, cost is low, fertilizer efficiency good, free from environmental pollution.
To the above-mentioned explanation of the disclosed embodiments, professional and technical personnel in the field are realized or uses the present invention.To be apparent for those skilled in the art to the multiple amendment of these embodiments, General Principle as defined herein can without departing from the spirit or scope of the present invention, realize in other embodiments.Therefore, the present invention can not be restricted to these embodiments shown in this article, but will meet the widest scope consistent with principle disclosed herein and features of novelty.