CN104678116A - Control system and control method of integrated quantitative sampling reagent adding device detector - Google Patents

Control system and control method of integrated quantitative sampling reagent adding device detector Download PDF

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Publication number
CN104678116A
CN104678116A CN201510082701.9A CN201510082701A CN104678116A CN 104678116 A CN104678116 A CN 104678116A CN 201510082701 A CN201510082701 A CN 201510082701A CN 104678116 A CN104678116 A CN 104678116A
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circuit
micro
processor mcv
sampling thief
reaction cup
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CN201510082701.9A
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Chinese (zh)
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郝书顺
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Shijiazhuang Hipro Biotechnology Co Ltd
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Shijiazhuang Hipro Biotechnology Co Ltd
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Abstract

The invention discloses a control system and a control method of an integrated quantitative sampling reagent adding device detector. The control system comprises a reaction cup induction detection circuit, a temperature control unit, a sampler locating pressing linkage control unit composed of a linkage position detection circuit and a sampler shifting pressing control circuit, a blending and light path locating unit composed of a blending control circuit and a light path locating detection circuit, a signal acquisition unit composed of 1-3 light signal detection circuits and an analog-to-digital (A/D) conversion circuit, a main control unit comprising a microprocessor (MCU), a detection information reading module and a display circuit. The control method comprises the steps of power-on self-testing, constant-temperature control, reaction cup placement detection, detection information reading, reaction cup temperature detection, pre-pressing, primary pressing, secondary pressing, detection result displaying, calibrating, quality correction and removal of the reaction cup. The control system and the control method disclosed by the invention can integrate various detection modes to achieve POCT automatic detection, calibration and quality correction in a whole course, so as to guarantee the reliability and the accuracy of a detection result.

Description

The control system of integrated quantitative sampling reagent adding device detector and control method
Technical field
Originally relate to a kind of control system and control method of sampling thief pick-up unit, especially the control system of integrated quantitative sampling reagent adding device detector and control method, be applicable to controlling biological monitoring and clinical medicine optical evaluation facility carries out pattern detection.
Background technology
Real-time test (POCT) carries out pattern detection analysis at once in sampling location, save the complex process program of sample when laboratory inspection, assay can be obtained fast, along with development and the medical science applied progress of new and high technology, have experimental apparatus miniaturization, simple operation, just-in-time of reporting the result POCT more and more receive the favor of people.
Chinese patent " Integral sample collecting injection device " (Authorization Notice No. is CN202204706U), achieves accurate sampling, storage and quantitatively adds the integrated function of reagent.Chinese patent " a kind of integrated quantitative sampling reagent adding device " (Authorization Notice No. is 102072949B), discloses a kind of integrated quantitative sampling reagent adding device, improves accuracy of detection.Chinese patent " a kind of reaction cup " (Authorization Notice No. is ZL201120293301.X) proposes a kind of reaction cup being arranged with a pair lug at rim of a cup place, can provide support, using convenient when annotating sample for finger.Chinese patent " a kind of hardware system of Immunofluorescence test instrument " (Authorization Notice No. is CN203606362U), achieves the transmission of fluorescent test paper strip, the detection of fluorescence reaction, the analysis of fluorescence signal, the printing of testing result and testing process networked function.These new technologies appear at the accuracy, the reliability that improve testing result to a certain extent.But, testing product is semiautomatic equipment both at home and abroad at present, from reagent information scanning, the detection of reaction cup initial temperature, constant temperature system start and stop, sample mixing, piston presses down, test pattern is switched to reagent test, need the artificial link participated in more in the middle of display operating process, and the manual operation error of bringing into exists very important impact for the accuracy of testing result, reliability.And prior art, only for the design of single test methodology, can only use a kind of mode detection sample, range of application is smaller.Hospital need purchase the demand that multiple stage instrument could meet multiple detection method, adds the cost of equipment purchasing.In addition, checkout equipment calibration and Quality Control course of work complexity, later stage instrument maintenance maintenance work is more loaded down with trivial details.
Summary of the invention
The invention provides a kind of control system and the control method that can carry out the integrated quantitative sampling reagent adding device detector of whole-course automation detection control.
For solving the problems of the technologies described above, the technical solution used in the present invention is:
Technical scheme one: a kind of control system of integrated quantitative sampling reagent adding device detector
Comprise main control unit, temperature control unit, comprise reaction cup induction testing circuit, Detection Information read module, sampling thief location press down coordinated signals unit, mixing and light path positioning unit, signal gathering unit and display circuit;
Described main control unit comprises Micro-processor MCV;
Described temperature control unit is made up of constant temperature control circuit, temperature sensing circuit and infrared temperature testing circuit; The respective input of Micro-processor MCV described in the output termination of described temperature sensing circuit; The corresponding output end of Micro-processor MCV described in the respective input of described constant temperature control circuit, its corresponding output end is for controlling the duty of electric heater and fan in described sampling thief sample automatic detection device; The respective input of Micro-processor MCV described in the output termination of described infrared temperature testing circuit;
The respective input of Micro-processor MCV described in the output termination of described reaction cup induction testing circuit;
The respective input of described Detection Information read module and output terminal connect corresponding output end and the input end of described Micro-processor MCV respectively;
Described sampling thief location presses down coordinated signals unit and is shifted by link position detecting circuit and sampling thief and presses down control circuit and form; The corresponding output end of described interlock position detecting circuit connects the respective input of described Micro-processor MCV; The respective input that described sampling thief displacement presses down control circuit connects the corresponding output end of described Micro-processor MCV, and its output terminal is for controlling rotation direction and the speed of drive motor in described sampling thief sample automatic detection device;
Described mixing and light path positioning unit are by mixing control circuit and light path positioning detecting circuit forms; The corresponding output end of described light path positioning detecting circuit connects the respective input of described Micro-processor MCV; The respective input of described mixing control circuit connects the corresponding output end of described Micro-processor MCV, and its output terminal is for controlling the rotation direction and the speed that mix motor in described sampling thief sample automatic detection device; Described light path positioning detecting circuit is made up of the first to the 3rd hall sensing circuit; The respective input of the described Micro-processor MCV of output termination difference of the described first to the 3rd hall sensing circuit;
Described signal gathering unit is made up of signal deteching circuit and A/D change-over circuit; Described signal deteching circuit is made up of fluorescence signal testing circuit, absorbance signal testing circuit and scattered light signal testing circuit; The output terminal of described fluorescence signal testing circuit, absorbance signal testing circuit and scattered light signal testing circuit connects the respective input of described A/D change-over circuit respectively, and the respective input of described fluorescence signal testing circuit, absorbance signal testing circuit, scattered light signal testing circuit and A/D change-over circuit connects the corresponding output end of described Micro-processor MCV respectively;
The corresponding output end of described display circuit and input end connect respective input and the output terminal of described Micro-processor MCV respectively.
Described signal deteching circuit is made up of any one in fluorescence signal testing circuit, absorbance signal testing circuit and scattered light signal testing circuit; Described light path positioning detecting circuit is made up of the first hall sensing circuit.
Described signal deteching circuit is made up of any two in fluorescence signal testing circuit, absorbance signal testing circuit and scattered light signal testing circuit; Described light path positioning detecting circuit is made up of the first to the second hall sensing circuit.
Further, the control system of described integrated quantitative sampling reagent adding device detector also comprises bluetooth module; Input end and the output terminal of described bluetooth module are connected corresponding output end and the input end of described Micro-processor MCV respectively; Described display circuit is LCD display curtain; Described information reading module is Quick Response Code read module or bar code read module.
Described interlock position detecting circuit is made up of identical the first angle sensor circuit of structure and the second angle sensor circuit; Input end and the output terminal of described first angle sensor circuit and the second angular transducer are connected corresponding output end and the input end of described Micro-processor MCV respectively; Described first angle sensor circuit comprises the first angular transducer; Described second angle sensor circuit comprises the second angular transducer; Described first angular transducer and the second angular transducer be arranged at respectively described sampling thief sample automatic detection device driven wheel 3 and from driven wheel 2-21 axis.
The displacement of described sampling thief presses down control circuit and is made up of the first photoelectric coupled circuit of cascade and the first driving circuit; The respective input of described first photoelectric coupled circuit connects the corresponding output end of described Micro-processor MCV, and its corresponding output end connects the respective input of described first driving circuit; The output terminal of described first driving circuit is for controlling described drive motor.
Described mixing control circuit is made up of the second photoelectric coupled circuit of cascade and the second driving circuit; The corresponding output end of Micro-processor MCV described in the input termination of described second photoelectric coupled circuit, its corresponding output end connects the respective input of described second driving circuit; The output terminal of described second driving circuit is for controlling described mixing motor.
Described fluorescence signal testing circuit is made up of the fluorescence signal radiating circuit connected by fluorescence light-path and fluorescence signal receiving circuit; Described fluorescence signal radiating circuit is made up of xenon lamp and fluorescence signal on-off circuit; Described fluorescence signal receiving circuit is made up of fluorescence light sensor circuit and programmable amplifying circuit; The respective input of described programmable amplifying circuit connects the corresponding output end of described fluorescence light sensor circuit and described Micro-processor MCV respectively, and its output terminal connects the respective input of described Micro-processor MCV through described A/D change-over circuit; The corresponding output end of Micro-processor MCV described in the input termination of described fluorescence signal radiating circuit.
Described absorbance signal testing circuit is made up of the absorbance signal radiating circuit connected by extinction light-path and absorbance signal receiving circuit; Described absorbance signal radiating circuit is made up of xenon lamp and absorbance signal on-off circuit; Described absorbance signal receiving circuit is made up of light sensor D9, operational amplifier U15, digital regulation resistance U14, resistance R23-R24 and electric capacity C3-C7, C16; Between 2 pin that described extinction light sensor D9 is connected on described operational amplifier U15 and ground, 4 pin of described operational amplifier U15,7 pin and 8 pin connect VCC3 power supply, VCC4 VDD-to-VSS respectively, and described VCC3 power supply and VCC4 power supply are respectively-5v and+5v power supply; Between 4 pin being connected on described operational amplifier U15 after described electric capacity C5 and C7 parallel connection and ground, between 7 pin being connected on described operational amplifier U15 after described electric capacity C4 and C16 parallel connection and 8 pin, be connected between 6 pin of described digital regulation resistance U14 and 2 pin of described operational amplifier U15 after described resistance R23 and electric capacity C6 parallel connection; 8 pin of described digital regulation resistance U14 connect VCC power supply, and described VCC power supply is+5v power supply; Between 8 pin that described electric capacity C3 is connected on described digital regulation resistance U14 and ground; Between 5 pin that described resistance R24 is connected on described digital regulation resistance U14 and ground, 7 pin of described digital regulation resistance U14 are connected with 6 pin of described operational amplifier U15 and are connected with the respective input of described A/D change-over circuit afterwards, the corresponding output end of Micro-processor MCV described in its 1-3 pin.
Described scattered light signal testing circuit is made up of the scattered light signal radiating circuit connected by scattered light light-path and scattering absorbance signal receiving circuit; Described scattered light signal radiating circuit is made up of laser tube and scattered light signal on-off circuit; Described scattered light signal receiving circuit is identical with described absorbance signal receiving circuit structure.Described infrared temperature testing circuit is made up of the infrared temperature sensing circuit of cascade and differential amplifier circuit; The respective input of Micro-processor MCV described in the output termination of described differential amplifier circuit.
Described reaction cup induction testing circuit is made up of the correlation sensing circuit of cascade and voltage comparator circuit; The respective input of Micro-processor MCV described in its output termination of described voltage comparator circuit.
Technical scheme two: a kind of control method utilizing described control system to control integrated quantitative sampling reagent adding device detector:
Be made up of following concrete steps:
A. startup self-detection: whether described Micro-processor MCV detects described sampling thief location and press down coordinated signals unit, mixing and the circuit of light path positioning unit and temperature control unit and normally work; If there is exception, described display circuit display alarm information, otherwise, perform step B;
The concrete steps of described startup self-detection are:
A-1. described Micro-processor MCV reads the reaction cup pallet 5-9 vertical motion positional information that described interlock position detecting circuit detects, calculates its initial position and mixing position difference, determines described drive motor rotation direction and speed; Control described sampling thief displacement press down control circuit control described drive motor running, make described reaction cup pallet 5-9 vertically move to mixing position;
A-2. under described Micro-processor MCV controls, described mixing control circuit controls described mixing motor rotation, drives sampler holder 3-2 to vacillate now to the left, now to the right; At the end of waving, described sampler holder 3-2 is swung to vertical direction;
A-3. described Micro-processor MCV reads the described reaction cup pallet 5-9 vertical motion positional information that described interlock position detecting circuit detects, calculates it and waits for that reaction cup puts into position difference, determining drive motor rotation direction and speed; Under described Micro-processor MCV controls, described sampling thief displacement presses down control circuit and controls the running of described drive motor, described reaction cup pallet 5-9 is moved to and waits for that reaction cup puts into position, open door simultaneously;
A-4. said process is if there is exception, controls described display circuit display alarm information;
B. thermostatic control: described Micro-processor MCV controls described sampling thief sample automatic detection device internal temperature constant temperature within the steady temperature deviation range preset;
B-1: described temperature sensing circuit reads described sampling thief sample automatic detection device inner air channel 6-4 outlet temperature, judges whether it is within the inner steady temperature deviation range of described sampling thief sample automatic detection device; If not, step B-2 is performed; If so, step C is performed;
B-2: judge whether the mxm. higher than steady temperature deviation range in described machine, if perform step B-3; If not, step B-4 is performed;
B-3: the built-in temperature of sampling thief pattern detection control device described in cycle detection, until it is lower than the mxm. of steady temperature deviation range in described machine, then performs step C;
B-4: start described constant temperature control circuit and control the built-in temperature that heating system heats Posterior circle detects described sampling thief pattern detection control device, until the internal temperature of described sampling thief sample automatic detection device is higher than the minimum of steady temperature deviation range in described machine, stop described constant temperature control circuit work, perform step C;
C. reaction cup inserts detection: described in described Micro-processor MCV cycle detection, whether sampling thief sample automatic detection device puts into reaction cup, until detect that rear execution step D is put in reaction;
D. Detection Information reads: under described Micro-processor MCV controls, and under described sampling thief location presses down coordinated signals unit controls, described drive motor running makes described reaction cup pallet 5-9 vertically be moved upwards up to information load position; Described Micro-processor MCV controls the Detection Information in described Detection Information read module reading reaction cup; Described Detection Information comprises and waves number of times, mode of operation, calibration curve, if mode of operation is calibration mode or matter school pattern, continues to read calibration figure;
E. reaction cup temperature detection: under described Micro-processor MCV controls, described sampling thief location presses down drive motor running described in coordinated signals unit controls, makes described reaction cup pallet 5-9 vertically be moved down into mixing position; The temperature of the described reaction cup that described infrared temperature testing circuit gathers; Described reaction cup temperature is read in described Micro-processor MCV circulation, until it is higher than the temperature of reaction preset, then performs step F;
F. mode of operation judges: if the mode of operation that described Detection Information read module reads is for detecting pattern or matter school pattern, perform step G, if be calibration mode, and execution step J;
G precompressed: under described Micro-processor MCV controls, described sampling thief location presses down drive motor running described in coordinated signals unit controls, band dynamic pressure plate 2-18 vertically moves downward, and by described sampling thief sample top vertically to pressing to precompressed position, then turns back to mixing position; Described mixing and light path positioning unit control to mix motor rotation, described sampling thief 8 being vacillated now to the left, now to the right according to waving number of times in described Detection Information together with reaction cup, resting on vertical position at the end of waving;
H. once press down: under described Micro-processor MCV controls, described sampling thief location presses down drive motor running described in coordinated signals unit controls, band dynamic pressure plate 2-18 vertically moves downward, and by described sampling thief sample top vertically to pressing to a depressed position, then turns back to mixing position; A described depressed position is lower than described precompressed position; Described mixing and light path positioning unit control described mixing motor rotation, described sampling thief 8 being vacillated now to the left, now to the right according to waving number of times in described Detection Information together with reaction cup, resting on vertical position at the end of waving;
I. press down for two times: under described Micro-processor MCV controls, described sampling thief location presses down the running of coordinated signals unit controls drive motor, band dynamic pressure plate 2-18 vertically moves downward, and by described sampling thief 8 top vertically to pressing to secondary depressed position, then turns back to mixing position; Described secondary depressed position is lower than a described depressed position; Described mixing and light path positioning unit control described mixing motor rotation, described sampling thief 8 being vacillated now to the left, now to the right according to waving number of times in described Detection Information together with reaction cup, resting on vertical position at the end of waving;
J. detect and show with result: described Micro-processor MCV reads in the detected signal value that described signal gathering unit exports, calculate in conjunction with described calibration curve and detect concentration of specimens, control described display circuit display testing result; By described bluetooth module, testing result is uploaded to flat board/mobile phone;
L. calibrate adjustment: if mode of operation is calibration mode, according to the difference of testing result and calibration figure, adjust the enlargement factor of described signal gathering unit, then perform step M; Otherwise, directly perform step M;
M. matter school process: if mode of operation is matter school pattern, compare testing result and calibration figure, if there are differences, according to calibration curve after calibration figure and testing result calculating matter school, and preserves, then performs step N; Otherwise, directly perform step N;
N. reaction cup is exited: described Micro-processor MCV controls mixing and drives described mixing motor that sampler holder 3-2 is swung to vertical state with light path conversion unit, control described sampling thief location again to press down coordinated signals unit controls drive motor and drive and press down tooth bar 2-1 and be vertically moved upwards up to out bin location, return step C.
The beneficial effect adopting technique scheme to produce is:
1, control system of the present invention achieves omnidistance POCT and automatically controls, and avoids manual procedure loaded down with trivial details in immunoassay procedures, improves test accuracy, accelerates test speed.
2, detection side's science of law that control system integration of the present invention three kinds is different, can carry out fluoroscopic examination, scattered light detects and absorb light and detect, and detecting pattern is selected automatically, reduce ATE (automatic test equipment) manufacture and maintenance cost.
3, adopt control method of the present invention, conveniently can carry out instrumental correction and Quality Control and correct, efficiently solve calibration and Quality Control Quality Control corrects loaded down with trivial details problem, ensure that the reliability of testing result, accuracy.
Accompanying drawing explanation
Below in conjunction with the drawings and specific embodiments to being originally described in further detail.
Fig. 1 is the functional-block diagram of control system of the present invention;
Fig. 2 is that the embodiment of the present invention 1 links the functional-block diagram of position detecting circuit;
Fig. 3 is that the embodiment of the present invention 1 links the circuit theory diagrams of position detecting circuit;
Fig. 4 is the functional-block diagram that the displacement of the embodiment of the present invention 1 sampling thief presses down control circuit;
Fig. 5 is the circuit theory diagrams that the displacement of the embodiment of the present invention 1 sampling thief presses down control circuit;
Fig. 6 is the functional-block diagram that the embodiment of the present invention 1 mixes control circuit;
Fig. 7 is the circuit theory diagrams that the embodiment of the present invention 1 mixes control circuit;
Fig. 8 is the functional-block diagram of the embodiment of the present invention 1 light path positioning detecting circuit;
Fig. 9 be the embodiment of the present invention 1 light path positioning detecting circuit circuit theory diagrams;
Figure 10 is the functional-block diagram of the embodiment of the present invention 1 signal deteching circuit;
Figure 11 is the functional-block diagram of the embodiment of the present invention 1 fluorescence signal testing circuit;
Figure 12 is the functional-block diagram of the embodiment of the present invention 1 absorbance signal testing circuit;
Figure 13 is the functional-block diagram of the embodiment of the present invention 1 scattered light signal testing circuit;
Figure 14 is the circuit theory diagrams of the embodiment of the present invention 1 fluorescence signal radiating circuit, scattered light signal radiating circuit and absorbance signal radiating circuit;
Figure 15 is the circuit theory diagrams of embodiment of the present invention 1A/D change-over circuit;
Figure 16 is the circuit theory diagrams of the embodiment of the present invention 1 fluorescence signal receiving circuit;
Figure 17 is the circuit theory diagrams of the embodiment of the present invention 1 fluorescence signal receiving circuit;
Figure 18 is the circuit theory diagrams of the embodiment of the present invention 1 absorbance signal testing circuit;
Figure 19 is the circuit theory diagrams of the embodiment of the present invention 1 temperature sensing circuit;
Figure 20 is the circuit theory diagrams of the embodiment of the present invention 1 constant temperature control circuit;
Figure 21 is the functional-block diagram of the embodiment of the present invention 1 reaction cup induction testing circuit;
Figure 22 is the circuit theory diagrams of the embodiment of the present invention 1 reaction cup induction testing circuit;
Figure 23 is the functional-block diagram of the embodiment of the present invention 1 infrared temperature detecting unit;
Figure 24 is the circuit theory diagrams of the embodiment of the present invention 1 infrared temperature detecting unit;
Figure 25 is the circuit theory diagrams of the embodiment of the present invention 1 bluetooth module;
Figure 26 is the circuit theory diagrams of the embodiment of the present invention 1 Quick Response Code read module;
Figure 27 is the circuit theory diagrams of the embodiment of the present invention 1 display circuit;
Figure 28 is the circuit theory diagrams of the embodiment of the present invention 1 main control unit;
Figure 29 is the structural representation of institute of the present invention control device;
Figure 30 is the sampling thief bracket initial position structural representation of institute of the present invention control device;
Figure 31 is the sampling thief carrier structure schematic diagram of institute of the present invention control device;
Figure 32 be institute of the present invention control device press down tooth bar structural representation;
Figure 33 is that the tooth bar that presses down of institute of the present invention control device prolongs horizontal carriage pressing structure schematic diagram;
Figure 34 is the heating system structural representation of institute of the present invention control device.
In accompanying drawing, 1 housing, 2 frames, 3 driven wheels, 5 drive motor, 1-1 xenon lamp, 1-2 fluorescence light sensor, 1-3 LCD display curtain, 2-1 presses down tooth bar, 2-7 horizontal carriage, 2-9 presses down linked gear, 2-10 presses down follower gear, 2-11 notch, 2-12 guide plate, 2-13 guide runner, 2-14 vertical supporting plate, 2-15 hypodontia, 2-16 is protruding, 2-18 pressing plate, 2-19 engaging tooth, 2-20 base plate, 2-21 is from driven wheel, 3-1 mixes motor, 3-2 sampling thief bracket, 3-3 screens reed, 3-4 bracing frame, 3-5 bearing, 3-6 gudgeon, 3-7 gathering sill, 3-8 fixed head, 3-11 lifter rack frame, 3-12 lifter rack, 3-14 chute, 3-15 spacing preiection, 3-16 base, 4-1 door, 5-1 guide groove, 5-2 presses lobe, 5-3 return spring, 5-4 follower gear, 5-9 reaction cup pallet, 6 Quick Response Code reading devices, 6-1 electric heater, 6-2 heat radiator, 6-3 fan, 6-4 air delivery duct, 8-sampling thief.
Embodiment
Following embodiment 1-4 is the embodiment of control system of the present invention, and embodiment 5 is the embodiment of control method of the present invention.
Embodiment 1:
The present embodiment 1 controls sampling thief sample automatic detection device and completes testing to sample in sampling thief.The sampling thief that sampling thief sample automatic detection device and Chinese patent " integral collecting charging point " (Authorization Notice No. is CN202204706U) are recorded and the reaction cup that Chinese patent " reaction cup " (Authorization Notice No. is ZL201120293301.X) is recorded with the use of.Reaction cup posts bar code information.
Sampling thief sample automatic detection device shown in Figure 29, comprising: housing 1, the frame 2 be arranged in housing, vertical detent mechanism, mixing mechanism, press mechanism and the control system be fixedly installed in frame 2.
In sampling thief bracket initial position structure shown in Figure 30 vertical detent mechanism comprise drive motor 5, the driven wheel 3 that is fixedly connected with drive motor 5 output shaft, be hinged on frame 2 engages with driven wheel 3 from driven wheel 2-21 and the lifter rack frame 3-11 that is arranged on by fixed head 3-8 frame 2, fixed head 3-8 is fixedly installed on base 3-16, lifter rack frame 3-11 is provided with the lifter rack 3-12 engaged with follower gear 5-4, and lifter rack frame 3-11 can along fixed head 3-8 vertical sliding motion.Follower gear 5-4 is provided with pressure lobe 5-2, and 5-2 is corresponding with lifter rack 3-12 top for pressure lobe, pressure lobe 5-2 one end and follower gear 5-4 hinged, the other end passes through return spring 5-3 and is connected with follower gear 5-4.Drive motor 5 output shaft is connected with driven wheel 3 by gear reduction.Lifter rack frame 3-11 is respectively arranged with lifter rack 3-12 in frame 2 both sides.
In sampling thief carrier structure shown in Figure 31, mixing mechanism comprises mixing motor 3-1, the sampling thief bracket 3-2 that its power output shaft is fixedly connected with, sampling thief bracket 3-2 is arranged at the gudgeon 3-6 on fixed head 3-8, the power output shaft of mixing motor 3-1 is coaxially fixedly connected with the gudgeon 3-6 of sampling thief bracket 3-2.As shown in figure 27, lifter rack frame 3-11 is provided with the reaction cup pallet 5-9 lifting sampling thief 8, and reaction cup pallet 5-9 is through sampling thief bracket 3-2, and what form sampling thief 8 with sampling thief bracket 3-2 accommodates space.Sampling thief bracket 3-2 is provided with the up and down guide groove 5-1 of reaction cup pallet 5-9, and Open Side Down for guide groove 5-1.
The press mechanism in rack structure that presses down as shown in figure 32 comprises: for pressing down the pressing plate 2-18 of sampling thief sample, bindiny mechanism and driving mechanism, pressing plate 2-18 is connected with driving mechanism by bindiny mechanism.Bindiny mechanism comprises: horizontal carriage 2-7 and be arranged at horizontal carriage 2-7 presses down tooth bar 2-1.The bottom of horizontal carriage 2-7 is base plate 2-20, is provided with the notch 2-11 for setting with the protruding 2-16 of driving mechanism above base plate 2-20; Press down tooth bar 2-1 to move up and down along horizontal carriage 2-7.As shown in figure 30, pressing down tooth bar 2-1 is frame-type structure, and its top is provided with pressing plate 2-18, side is provided with engaging tooth 2-19, opposite side is provided with guide plate 2-12 for leading.
Driving mechanism as shown in Figure 30 and Figure 33 comprise driven wheel 3, engage with driven wheel 3 press down follower gear 2-10, with press down that follower gear 2-10 coaxially arranges press down linked gear 2-9, press down follower gear 2-10 and press down linked gear 2-9 sidepiece and be provided with protruding 2-16, protruding 2-16 is together with notch 2-11 inlay card, and horizontal carriage 2-7 can be driven to move forward and backward, press down follower gear 2-10 and press down linked gear 2-9 and to drive through engaging tooth 2-19 and press down tooth bar 2-1 and move up and down.
Sampling thief sample automatic detection device as shown in figure 34 also comprise for housing 1 inside heat up electric heater 6-1, with the heat radiator 6-2 that electric heater 6-1 is fixedly connected with, be fixed on heat radiator 6-2 side fan 6-3 and with fan 6-3 the corresponding air delivery duct 6-4 arranged, air delivery duct 6-4 air outlet is arranged on below sampling thief bracket 3-2.
As shown in Figure 1, embodiment 1 comprise reaction cup induction testing circuit, temperature control unit, sampling thief location press down coordinated signals unit, mixing and light path positioning unit, signal gathering unit, main control unit, Detection Information read module, display circuit and bluetooth module.
Main control unit comprises Micro-processor MCV; Micro-processor MCV model is STM32F103, sees Figure 28.
Described temperature control unit is made up of constant temperature control circuit, temperature sensing circuit and infrared temperature testing circuit; The respective input of Micro-processor MCV described in the output termination of described temperature sensing circuit; The corresponding output end of Micro-processor MCV described in the respective input of described constant temperature control circuit, its corresponding output end is for controlling the duty of electric heater and fan in described sampling thief sample automatic detection device; The respective input of Micro-processor MCV described in the output termination of described infrared temperature testing circuit;
The respective input of Micro-processor MCV described in the output termination of described reaction cup induction testing circuit;
The respective input of described Detection Information read module and output terminal connect corresponding output end and the input end of described Micro-processor MCV respectively;
Described sampling thief location presses down coordinated signals unit and is shifted by link position detecting circuit and sampling thief and presses down control circuit and form; The corresponding output end of described interlock position detecting circuit connects the respective input of described Micro-processor MCV; The respective input that described sampling thief displacement presses down control circuit connects the corresponding output end of described Micro-processor MCV, and its output terminal is for controlling rotation direction and the speed of drive motor in described sampling thief sample automatic detection device;
Described mixing and light path positioning unit are by mixing control circuit and light path positioning detecting circuit forms; The corresponding output end of described light path positioning detecting circuit connects the respective input of described Micro-processor MCV; The respective input of described mixing control circuit connects the corresponding output end of described Micro-processor MCV, and its output terminal is for controlling the rotation direction and the speed that mix motor in described sampling thief sample automatic detection device; Described light path positioning detecting circuit is made up of the first to the 3rd hall sensing circuit; The respective input of the described Micro-processor MCV of output termination difference of the described first to the 3rd hall sensing circuit;
Described signal gathering unit is made up of signal deteching circuit and A/D change-over circuit; Described signal deteching circuit is made up of fluorescence signal testing circuit, absorbance signal testing circuit and scattered light signal testing circuit; The output terminal of described fluorescence signal testing circuit, absorbance signal testing circuit and scattered light signal testing circuit connects the respective input of described A/D change-over circuit respectively, and the respective input of described fluorescence signal testing circuit, absorbance signal testing circuit, scattered light signal testing circuit and A/D change-over circuit connects the corresponding output end of described Micro-processor MCV respectively;
The corresponding output end of described display circuit and input end connect respective input and the output terminal of described Micro-processor MCV respectively.
As shown in figure 21, reaction cup induction testing circuit is made up of the correlation sensing circuit of cascade and voltage comparator circuit.As shown in figure 22, reaction cup induction testing circuit is made up of light emitting diode D12, phototriode Q1, resistance R35-R36 and electric capacity C25; Voltage comparator circuit comprises voltage comparator U16 that model is TLV2371 and resistance R32-R34 and forms.Light emitting diode D12 and phototriode Q1 forms opposite type sensor.When reaction cup does not exist, the light that light emitting diode D12 launches shines directly on the receiving plane of phototriode Q1, phototriode Q1 conducting, the voltage that collector exports is lower, when the cup that responds exists, the light that light emitting diode D12 launches is blocked by reaction cup, and phototriode Q1 does not receive the light that light emitting diode D12 launches, be in not on-state, the voltage that its collector exports is higher.The voltage that phototriode Q1 exports connects voltage comparator U16 in-phase input end, if during higher than voltage comparator inverting input input voltage, voltage comparator U16 exports high level, represent that reaction cup exists, when the voltage that phototriode Q1 exports is lower than voltage comparator U16 inverting input input voltage, voltage comparator U16 represents to there is not reaction cup in OUT pin output low level, in the output of OUT pin, Micro-processor MCV 42 pin judges that the current cup that whether responds exists by reading U16.
Information in reaction cup is Quick Response Code, and Detection Information read module is Quick Response Code read module, and Micro-processor MCV reads in the output information of Quick Response Code read module by its 48,49 pin as serial ports use of 46 foot control systems, as shown in figure 21.
As shown in figure 19, temperature sensing circuit is 38 pin that the digital temperature sensor U22 of DS18B20 and 2 pin of resistance R55, DS18B20 connect Micro-processor MCV by model.Digital temperature sensor is arranged on the heat radiator 6-2 at 6-4 air outlet place, air channel.As shown in figure 20, constant temperature control circuit is made up of diode D14, field effect transistor Q2-Q3, resistance R56-R57 and binding post J4.The grid of field effect transistor Q2 and Q3 connects 3 pin and 2 pin of Micro-processor MCV respectively through resistance R56, R5.Binding post J4 is used for the red line of external electric heater 6-1 and fan 6-3, fan 6-3 and any pin of electric heater 6-1 connects 2 pin jointly, and the black line of fan 6-3 connects 1 pin, and another pin of electric heater 6-1 connects 3 pin.Temperature sensing circuit detects the portion's temperature in institute control device, cooperates with constant temperature control circuit, within the steady temperature deviation range making it be in preset.When temperature is in steady temperature deviation range, constant temperature control circuit enters holding state.As shown in figure 34, when described control device internal temperature is lower than pre-set constant temperature deviation scope minimum, described Micro-processor MCV controls constant temperature control circuit startup electric heater 6-1 and fan 6-3 and works, electric heater 6-1 heat is delivered to heat radiator 6-2, the fan 6-3 be arranged on heat radiator 6-2 rotates, and drives air to flow through heat radiator and exports through air channel.When temperature is equal to or higher than steady temperature deviation range mxm., under described Micro-processor MCV controls, constant temperature control circuit controls electric heater 6-1 and quits work.
As shown in figure 23, infrared temperature testing circuit is made up of infrared temperature sensing circuit and differential amplifier circuit.As shown in figure 24, differential amplifier circuit is that the differential amplifier U17 of INA145 and resistance R47, R51 form by model; Infrared temperature sensing circuit is made up of infrared temperature sensor U18, electric capacity C26 and resistance R48-R50.The model of infrared temperature sensor U18 is OTP-538, above the bearing seat being arranged on reaction cup pallet 5-9.The reaction cup temperature transition collected is simulating signal input difference amplifying circuit by infrared temperature sensing circuit, differential amplifier circuit is transferred to analog input end 16 pin of Micro-processor MCV after amplifying it, be converted to digital signal by the built-in A/D converter of processor.Infrared temperature sensor is used for detection reaction cup temperature, and when reaction cup temperature reaches default temperature of reaction, sampling thief sample automatic detection device starts to detect.
As shown in Figure 2, interlock position detecting circuit is used for detection and presses down tooth bar vertical motion position, and it comprises the first identical angle sensor circuit of structure and the second angle sensor circuit.First angle sensor circuit is connected the 83-88 pin of described microprocessor with the output terminal of the second angle sensor circuit as shown in Figure 3.As shown in Figure 3, the first angular transducer is made up of angular transducer U2, electric capacity C19-C20 and resistance R11-R13; Second angular transducer is made up of angular transducer U3, electric capacity C21-C22 and resistance R14-R16.The angular transducer of the first angle sensor circuit and the second angle sensor circuit is arranged at driven wheel 3 respectively and from the diverse location the axis of driven wheel 2-21, model is 316BDG.Be fixedly installed radial magnetizing magnet from one end of driven wheel 2-21 rotation axis, angular transducer is mated and detects driven wheel 3 and from driven wheel 2-21 angle in relative rotation, for calculating the vertical motion position determining reaction cup.
In the present embodiment, drive motor is direct current generator, and as shown in Figure 4, sampling thief displacement presses down the first photoelectric coupled circuit and the direct current motor drive circuit that control circuit comprises cascade; The output terminal of direct current motor drive circuit connects direct current generator.Drive motor can be also other kind motors, and as stepper motor, corresponding mixing motor-drive circuit is suitable with mixing motor type.As shown in Figure 5, the first photoelectric coupled circuit comprises the first optocoupler branch road, the second optocoupler branch road and the 3rd optocoupler branch road.First optocoupler props up route opto-coupler chip U4 and resistance R17, R20 composition; Second optocoupler props up route opto-coupler chip U5 and resistance R18, R21 composition; 3rd optocoupler props up route opto-coupler chip U6 and resistance R19, R22 composition.Direct current motor drive circuit is made up of DC motor Driver chip U1 and electric capacity C23-C24 and resistance R10.The model of opto-coupler chip U4-U6 is PS2805-1, and the model of DC motor Driver chip is LMD18200T.
As shown in Figure 27 and 29, under main control unit controls, sampling thief displacement presses down control circuit and controls drive motor 5, makes reaction cup pallet 5-9 or pressing plate 2-18 vertically move to the target location of specifying.Described Micro-processor MCV compares the relative position relation of current location and target location, determines rotation direction and the speed of drive motor.Micro-processor MCV exports the signal controlling direct current generator rotation direction by 35 pin, its 34 pin exports the enable signal of DC motor Driver chip, its 33 pin exports rotation direction and the speed running that PWM ripple controls drive motor 5, drive motor 5 drives driven wheel 3 to move, driven wheel 3 drives from driven wheel 2-21 action, the lifter rack 3-12 engaged with from driven wheel 2-21 rises or declines, lifter rack frame 3-11 is driven to rise or decline, reaction cup pallet 5-9 also rises thereupon or declines, and sees Figure 30.Meanwhile, Micro-processor MCV receives the reaction cup vertical motion positional information that interlock position detecting circuit gathers, and judges whether reaction cup pallet 5-9 or pressing plate arrive target location.
As shown in Figure 10, in the present embodiment, signal gathering unit is by fluorescence signal testing circuit, absorbance signal testing circuit and scattered light signal testing circuit group road and A/D change-over circuit.
As shown in figure 11, described fluorescence signal testing circuit is made up of the fluorescence signal radiating circuit connected by fluorescence light-path and fluorescence signal receiving circuit.As shown in figure 14, fluorescence signal radiating circuit is made up of the xenon lamp 1-1 be fixedly mounted in frame 2 and fluorescence signal on-off circuit, and described fluorescence signal on-off circuit is made up of triode Q4 and resistance R63, R68, and Micro-processor MCV is by its switch of 93 foot control systems.As shown in figure 14, fluorescence signal receiving circuit comprises fluorescence light sensor circuit and programmable amplifying circuit.The input end of programmable amplifying circuit connects the corresponding output end of fluorescence light sensor D10 and Micro-processor MCV respectively, and it exports the respective input connecing Micro-processor MCV.As shown in figure 15, programmable amplifying circuit is made up of programmable amplifier U11 and diode D11, and wherein the model of programmable amplifier U11 is IVC102.
As shown in figure 12, absorbance signal testing circuit is made up of absorbance signal radiating circuit and absorbance signal receiving circuit.As shown in figure 14, absorbance signal radiating circuit is fixedly mounted on xenon lamp 1-1, triode Q5 in frame 2 and resistance R64, R67 form by pipe.Binding post J6 for connecting xenon lamp connects Micro-processor MCV 92 pin through transistor switching circuit, controls its switch by Micro-processor MCV.As shown in figure 18, absorbance signal receiving circuit comprises extinction light sensor D9, operational amplifier U15, digital regulation resistance U14, resistance R23-R24 and electric capacity C3-C7, C16.Extinction light sensor D9 receives the light intensity signal that xenon lamp 1-1 launches; Extinction light sensor D1 is connected between 2 pin of operational amplifier U15 and ground, and 4 pin of operational amplifier U15,7 pin and 8 pin connect-5v ,+5v VDD-to-VSS respectively; Be connected between 4 pin of operational amplifier U15 and ground after electric capacity C5 and C7 parallel connection, be connected between 6 pin of operational amplifier U15 and 7 pin after electric capacity C4 and C16 parallel connection, after resistance R23 and electric capacity C6 parallel connection, be connected between 6 pin of digital regulation resistance U14 and 2 pin of operational amplifier U15; 8 pin of digital regulation resistance U14 connect+5v power supply, and electric capacity C3 is connected between 8 pin of digital regulation resistance U14 and ground; 8 pin of digital regulation resistance U14 connect 6 pin of digital regulation resistance U14,5 pin through resistance R24 ground connection, the corresponding output end of the 1-3 pin Micro-processor MCV of digital regulation resistance U14.
The light intensity signal received is converted to the electric current of corresponding size by extinction light sensor D9, and electric current amplifies via the amplifying circuit be made up of operational amplifier U15, digital regulation resistance U14, resistance R23-R24 and is converted to the 6 pin outputs of voltage signal by digital regulation resistance U14.Digital regulation resistance U14, in the feedback circuit of amplifying circuit, adjusts tap position and changes the enlargement factor of circuit to reach the function of adjustment gain under Micro-processor MCV controls.
As shown in figure 13, scattered light signal testing circuit is made up of the scattered light signal radiating circuit connected by scattered light light-path and scattering absorbance signal receiving circuit.As shown in figure 14, described scattered light signal radiating circuit is made up of laser tube and scattered light signal on-off circuit.Scattered light signal on-off circuit is made up of triode Q6 and resistance R65, R66.Second laser J7 connects Micro-processor MCV 91 pin through transistor switching circuit, controls its switch by Micro-processor MCV.Scattered light signal receiving circuit circuit structure is identical with absorbance signal receiving circuit with principle of work.
As shown in figure 15, A/D change-over circuit is made up of A/D converter U12, active crystal oscillator Y1, reference power supply U13, resistance R25-R30 and electric capacity C8-C11, C13-C15, C17-C18.Wherein the model of A/D converter U12, reference power supply U13 is respectively ADS1253, LM4040-4.1.
Mixing control circuit is used under Micro-processor MCV controls, and controls mixing driven by motor sampling thief bracket 3-2 and swings centered by vertical direction.In the present embodiment, mixing motor is stepper motor, and as shown in Figure 6, mixing control circuit comprises the second photoelectric coupled circuit and second driving circuit of cascade, and the output terminal of the second driving circuit connects stepper motor.Mixing motor can be also other kind motors, and as direct current generator, corresponding mixing motor-drive circuit is suitable with mixing motor type.As shown in Figure 7, the second photoelectric coupled circuit is made up of the 4th to the 6th optocoupler branch road.4th optocoupler props up route opto-coupler chip U8 and resistance R7, R34 composition, and the 5th optocoupler props up route opto-coupler chip U9 and resistance R8, R35 composition, and the 6th optocoupler props up route opto-coupler chip U10 and resistance R9, R36 composition.Second driving circuit is made up of stepper motor driver chip U7, resistance R37-R46, electric capacity C1-C2, diode D1-D8 and binding post J1.The model of stepper motor driver chip U7 is THB7128, and the model of opto-coupler chip U8-U10 is PS2805-1.
19 pin of stepper motor driver chip, 2 pin and 3 pin are connect respectively through the road road of the 4th to the 6th optocoupler at Micro-processor MCV 30-32 pin, the 2 pin input signals of motor drive ic U7 are for controlling the rotation direction mixing motor 3-1,3 pin input signals are for controlling the running speed mixing motor 3-1, and 19 pin input signals are for controlling the running and the stopping that mixing motor 3-1.As shown in Figure 8 and Figure 9, light path positioning detecting circuit comprises three are departed from vertical direction angle hall sensing circuit for detection reaction cup, first hall sensing circuit is made up of Hall element S1 and resistance R1, and wherein the model of Hall element S1 is HAL3144.First Hall element is arranged on fluorescence light-path, and the Hall element S2 in the second hall sensing circuit installs on extinction light-path, and in the 3rd hall sensing circuit, Hall element S3 is arranged on scattered light light-path.
As shown in figure 31, between mixing motor 3-1 on-stream period, Micro-processor MCV reads the output signal of the Hall element in the light path positioning detecting circuit of road by circulation, calculate the swing position determining to mix motor, control mixing motor 3-1 running, integrated sampling charging point sampling thief bracket 3-2 being driven insert wherein and reaction cup close-connected with it arrive the target location of specifying.
Display circuit is LED display curtain, connects Micro-processor MCV mouth 1 pin and 94-97 pin, as shown in figure 27.
Detection Information on sampling thief sample is Quick Response Code, and Detection Information reading device is the Quick Response Code reading device 6 being fixedly mounted on bracing frame 3-4 top.
Micro-processor MCV controls bluetooth module and connects flat board/mobile phone.
Embodiment 2:
The difference of the present embodiment and embodiment 1 is that light path testing circuit only includes a fluorescence signal testing circuit, and light path positioning detecting circuit only includes the first hall sensing circuit.Monitoring information in reaction cup is bar code, and Detection Information reading device is bar code.
Embodiment 3:
The difference of the present embodiment and embodiment 2 is that light path testing circuit is for only including an absorbance signal testing circuit.
Embodiment 4:
The difference of the present embodiment and embodiment 2 is that light path testing circuit is for only including a scattered light signal testing circuit.
Embodiment 5:
The control system of utilization controls a control method for described integrated quantitative sampling reagent adding device detector, comprises the following steps:
A. startup self-detection: Micro-processor MCV detection sampling thief is located and pressed down coordinated signals unit, mix and whether the circuit of light path positioning unit and temperature control unit normally works; If there is exception, control display circuit display alarm information, otherwise, perform step B;
Steps A comprises step by step following:
A-1. Micro-processor MCV reads the reaction cup pallet 5-9 vertical motion positional information that interlock position detecting circuit detects, calculates its initial position and mixing position difference, determines drive motor rotation direction and speed; Control sampling thief displacement and press down the running of control circuit control drive motor, make reaction cup vertically move to mixing position;
A-2., under Micro-processor MCV controls, mixing control circuit controls mixing motor rotation, makes sampler holder 3-2 wave number of times according to the self-inspection preset and vacillates now to the left, now to the right; In rocking process, the sampling thief sample rocked position information that in light path positioning detecting circuit, the first Hall angle sensing circuit gathers is read in circulation, and that determines to have completed waves number of times; At the end of waving, sampler holder 3-2 is swung to vertical direction;
A-3. Micro-processor MCV reads the reaction cup pallet 5-9 vertical motion positional information that interlock position detecting circuit detects, calculates it and waits for that reaction cup puts into position difference, determining drive motor rotation direction and speed; Under Micro-processor MCV controls, sampling thief displacement presses down control circuit and controls drive motor running, reaction cup pallet 5-9 is moved to and waits for that reaction cup puts into position, open door simultaneously;
A-4. said process is if there is exception, controls display circuit display alarm information;
B. thermostatic control: Micro-processor MCV controls sampling thief sample automatic detection device internal temperature constant temperature within the steady temperature deviation range preset;
B-1: temperature sensing circuit reads sampling thief sample automatic detection device inner air channel 6-4 outlet temperature, judges whether it is within the inner steady temperature deviation range of sampling thief sample automatic detection device; If not, step B-2 is performed; If so, step C is performed;
B-2: judge whether the mxm. higher than steady temperature deviation range in machine, if perform step B-3; If not, step B-4 is performed;
B-3: the built-in temperature of cycle detection sampling thief pattern detection control device, until it is lower than the mxm. of steady temperature deviation range in machine, then performs step C;
B-4: start constant temperature control circuit and control the built-in temperature that heating system heats Posterior circle detects sampling thief pattern detection control device, until reaction cup temperature is higher than the minimum of steady temperature deviation range in machine, stop constant temperature control circuit work, perform step C;
C. reaction cup inserts detection: whether Micro-processor MCV cycle detection sampling thief sample automatic detection device puts into reaction cup, until detect that rear execution step D is put in reaction;
D. Detection Information reads: under Micro-processor MCV controls, and under sampling thief location presses down coordinated signals unit controls, drive motor running makes reaction cup pallet 5-9 vertically be moved upwards up to information load position; Micro-processor MCV controls the Detection Information in Detection Information read module reading reaction cup; Detection Information comprise wave number of times, mode of operation, calibration curve, calibration mark and matter school mark; If calibration mark or matter school mark are effectively, continue to read calibration figure;
E. reaction cup temperature detection: under Micro-processor MCV controls, sampling thief location presses down the running of coordinated signals unit controls drive motor, makes reaction cup pallet 5-9 vertically be moved down into mixing position; The temperature of the reaction cup that infrared temperature testing circuit gathers; Reaction cup temperature is read in Micro-processor MCV circulation, until it is higher than the temperature of reaction preset, then performs step F;
F. precompressed: under Micro-processor MCV controls, sampling thief location presses down the running of coordinated signals unit controls drive motor, makes sampling thief sample top vertically be moved down into precompressed position, then turns back to mixing position; Mixing and light path positioning unit control to mix motor rotation, sampling thief sample being vacillated now to the left, now to the right according to waving number of times in Detection Information together with reaction cup, resting on vertical position at the end of waving;
G. once press down: under Micro-processor MCV controls, sampling thief location presses down the running of coordinated signals unit controls drive motor, makes sampling thief sample top vertically be moved down into a depressed position, then turns back to mixing position; A depressed position is lower than precompressed position; Mixing and light path positioning unit control to mix motor rotation, sampling thief sample being vacillated now to the left, now to the right according to waving number of times in Detection Information together with reaction cup, resting on vertical position at the end of waving;
H. press down for two times: under Micro-processor MCV controls, sampling thief location presses down the running of coordinated signals unit controls drive motor, makes sampling thief sample top vertically be moved down into secondary depressed position, then turns back to mixing position; A depressed position is higher than secondary depressed position; Mixing and light path positioning unit control to mix motor rotation, sampling thief sample being vacillated now to the left, now to the right according to waving number of times in Detection Information together with reaction cup, resting on vertical position at the end of waving;
I. testing result display: Micro-processor MCV reads in the output detections signal value of signal gathering unit, calculates in conjunction with calibration curve and detects concentration of specimens, controls display circuit display testing result; By bluetooth module, testing result is uploaded to flat board/mobile phone;
J. calibrate: Micro-processor MCV judges that calibration mark is whether effective, if effectively, according to the difference of testing result and calibration figure, adjust the enlargement factor of signal gathering unit, then perform step M; Otherwise, perform step K;
K. matter school: Micro-processor MCV judges that whether matter school mark is effective, if effectively, compares testing result and calibration figure, if there are differences, according to calibration curve after calibration figure and testing result calculating matter school, and preserves, then performs step L; Otherwise, perform step L;
L. reaction cup is exited: Micro-processor MCV controls mixing and drives with light path conversion unit and mix motor sampler holder 3-2 is swung to vertical state, control sampling thief location again to press down coordinated signals unit controls drive motor and drive and press down tooth bar 2-1 and be vertically moved upwards up to out bin location, return step C.
Above, be only this preferred embodiment, be not restriction this method being made to other form, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the Equivalent embodiments of equivalent variations.But everyly do not depart from the technical program content, any simple modification, equivalent variations and remodeling that the technical spirit according to this is done above embodiment, still belong to the protection domain of the technical program.

Claims (13)

1. a control system for integrated quantitative sampling reagent adding device detector, is characterized in that: comprise main control unit, temperature control unit, comprise reaction cup induction testing circuit, Detection Information read module, sampling thief location press down coordinated signals unit, mixing and light path positioning unit, signal gathering unit and display circuit;
Described main control unit comprises Micro-processor MCV;
Described temperature control unit is made up of constant temperature control circuit, temperature sensing circuit and infrared temperature testing circuit; The respective input of Micro-processor MCV described in the output termination of described temperature sensing circuit; The corresponding output end of Micro-processor MCV described in the respective input of described constant temperature control circuit, its corresponding output end is for controlling the duty of electric heater and fan in described sampling thief sample automatic detection device; The respective input of Micro-processor MCV described in the output termination of described infrared temperature testing circuit;
The respective input of Micro-processor MCV described in the output termination of described reaction cup induction testing circuit;
The respective input of described Detection Information read module and output terminal connect corresponding output end and the input end of described Micro-processor MCV respectively;
Described sampling thief location presses down coordinated signals unit and is shifted by link position detecting circuit and sampling thief and presses down control circuit and form; The corresponding output end of described interlock position detecting circuit connects the respective input of described Micro-processor MCV; The respective input that described sampling thief displacement presses down control circuit connects the corresponding output end of described Micro-processor MCV, and its output terminal is for controlling rotation direction and the speed of drive motor in described sampling thief sample automatic detection device;
Described mixing and light path positioning unit are by mixing control circuit and light path positioning detecting circuit forms; The corresponding output end of described light path positioning detecting circuit connects the respective input of described Micro-processor MCV; The respective input of described mixing control circuit connects the corresponding output end of described Micro-processor MCV, and its output terminal is for controlling the rotation direction and the speed that mix motor in described sampling thief sample automatic detection device; Described light path positioning detecting circuit is made up of the first to the 3rd hall sensing circuit; The respective input of the described Micro-processor MCV of output termination difference of the described first to the 3rd hall sensing circuit;
Described signal gathering unit is made up of signal deteching circuit and A/D change-over circuit; Described signal deteching circuit is made up of fluorescence signal testing circuit, absorbance signal testing circuit and scattered light signal testing circuit; The output terminal of described fluorescence signal testing circuit, absorbance signal testing circuit and scattered light signal testing circuit connects the respective input of described A/D change-over circuit respectively, and the respective input of described fluorescence signal testing circuit, absorbance signal testing circuit, scattered light signal testing circuit and A/D change-over circuit connects the corresponding output end of described Micro-processor MCV respectively;
The corresponding output end of described display circuit and input end connect respective input and the output terminal of described Micro-processor MCV respectively.
2. the control system of integrated quantitative sampling reagent adding device detector according to claim 1, is characterized in that: described signal deteching circuit is made up of any one in fluorescence signal testing circuit, absorbance signal testing circuit and scattered light signal testing circuit; Described light path positioning detecting circuit is made up of the first hall sensing circuit.
3. the control system of integrated quantitative sampling reagent adding device detector according to claim 1, is characterized in that: described signal deteching circuit is made up of any two in fluorescence signal testing circuit, absorbance signal testing circuit and scattered light signal testing circuit; Described light path positioning detecting circuit is made up of the first to the second hall sensing circuit.
4. the control system of the integrated quantitative sampling reagent adding device detector according to claim 1-3, is characterized in that: also comprise bluetooth module; Input end and the output terminal of described bluetooth module are connected corresponding output end and the input end of described Micro-processor MCV respectively; Described display circuit is LCD display curtain; Described information reading module is Quick Response Code read module or bar code read module.
5. the control system of the integrated quantitative sampling reagent adding device detector according to claim 1-3, is characterized in that: described interlock position detecting circuit is made up of identical the first angle sensor circuit of structure and the second angle sensor circuit; Input end and the output terminal of described first angle sensor circuit and the second angular transducer are connected corresponding output end and the input end of described Micro-processor MCV respectively; Described first angle sensor circuit comprises the first angular transducer; Described second angle sensor circuit comprises the second angular transducer; Described first angular transducer and the second angular transducer be arranged at respectively described sampling thief sample automatic detection device driven wheel 3 and from driven wheel 2-21 axis.
6. the control system of the integrated quantitative sampling reagent adding device detector according to claim 1-3, is characterized in that: described sampling thief displacement presses down control circuit and is made up of the first photoelectric coupled circuit of cascade and the first driving circuit; The respective input of described first photoelectric coupled circuit connects the corresponding output end of described Micro-processor MCV, and its corresponding output end connects the respective input of described first driving circuit; The output terminal of described first driving circuit is for controlling described drive motor.
7. the control system of the integrated quantitative sampling reagent adding device detector according to claim 1-3, is characterized in that: described mixing control circuit is made up of the second photoelectric coupled circuit of cascade and the second driving circuit; The corresponding output end of Micro-processor MCV described in the input termination of described second photoelectric coupled circuit, its corresponding output end connects the respective input of described second driving circuit; The output terminal of described second driving circuit is for controlling described mixing motor.
8. the control system of the integrated quantitative sampling reagent adding device detector according to claim 1-3, is characterized in that: described fluorescence signal testing circuit is made up of the fluorescence signal radiating circuit connected by fluorescence light-path and fluorescence signal receiving circuit; Described fluorescence signal radiating circuit is made up of xenon lamp (1-1) and fluorescence signal on-off circuit; Described fluorescence signal receiving circuit is made up of fluorescence light sensor circuit and programmable amplifying circuit; The respective input of described programmable amplifying circuit connects the corresponding output end of described fluorescence light sensor circuit and described Micro-processor MCV respectively, and its output terminal connects the respective input of described Micro-processor MCV through described A/D change-over circuit; The corresponding output end of Micro-processor MCV described in the input termination of described fluorescence signal radiating circuit.
9. the control system of the integrated quantitative sampling reagent adding device detector according to claim 1-3, is characterized in that: described absorbance signal testing circuit is made up of the absorbance signal radiating circuit connected by extinction light-path and absorbance signal receiving circuit; Described absorbance signal radiating circuit is made up of xenon lamp (1-1) and absorbance signal on-off circuit; Described absorbance signal receiving circuit is made up of light sensor D9, operational amplifier U15, digital regulation resistance U14, resistance R23-R24 and electric capacity C3-C7, C16; Between 2 pin that described extinction light sensor D9 is connected on described operational amplifier U15 and ground, 4 pin of described operational amplifier U15,7 pin and 8 pin connect VCC3 power supply, VCC4 VDD-to-VSS respectively, and described VCC3 power supply and VCC4 power supply are respectively-5v and+5v power supply; Between 4 pin being connected on described operational amplifier U15 after described electric capacity C5 and C7 parallel connection and ground, between 7 pin being connected on described operational amplifier U15 after described electric capacity C4 and C16 parallel connection and 8 pin, be connected between 6 pin of described digital regulation resistance U14 and 2 pin of described operational amplifier U15 after described resistance R23 and electric capacity C6 parallel connection; 8 pin of described digital regulation resistance U14 connect VCC power supply, and described VCC power supply is+5v power supply; Between 8 pin that described electric capacity C3 is connected on described digital regulation resistance U14 and ground; Between 5 pin that described resistance R24 is connected on described digital regulation resistance U14 and ground, 7 pin of described digital regulation resistance U14 are connected with 6 pin of described operational amplifier U15 and are connected with the respective input of described A/D change-over circuit afterwards, the corresponding output end of Micro-processor MCV described in its 1-3 pin.
10. the control system of the integrated quantitative sampling reagent adding device detector according to claim 1-3, is characterized in that: described scattered light signal testing circuit is made up of the scattered light signal radiating circuit connected by scattered light light-path and scattering absorbance signal receiving circuit; Described scattered light signal radiating circuit is made up of laser tube and scattered light signal on-off circuit; Described scattered light signal receiving circuit is identical with described absorbance signal receiving circuit structure.
The control system of 11. integrated quantitative sampling reagent adding device detectors according to claim 1-3, is characterized in that: described infrared temperature testing circuit is made up of the infrared temperature sensing circuit of cascade and differential amplifier circuit; The respective input of Micro-processor MCV described in the output termination of described differential amplifier circuit.
The control system of 12. integrated quantitative sampling reagent adding device detectors according to claim 1-3, is characterized in that: described reaction cup induction testing circuit is made up of the correlation sensing circuit of cascade and voltage comparator circuit; The respective input of Micro-processor MCV described in its output termination of described voltage comparator circuit.
13. 1 kinds of control methods utilizing the control system described in claim 1 to control described integrated quantitative sampling reagent adding device detector, is characterized in that: be made up of following steps:
A. startup self-detection: whether described Micro-processor MCV detects described sampling thief location and press down coordinated signals unit, mixing and the circuit of light path positioning unit and temperature control unit and normally work; If there is exception, described display circuit display alarm information, otherwise, perform step B;
The concrete steps of described startup self-detection are:
A-1. described Micro-processor MCV reads the reaction cup pallet 5-9 vertical motion positional information that described interlock position detecting circuit detects, calculates its initial position and mixing position difference, determines described drive motor rotation direction and speed; Control described sampling thief displacement press down control circuit control described drive motor running, make described reaction cup pallet 5-9 vertically move to mixing position;
A-2. under described Micro-processor MCV controls, described mixing control circuit controls described mixing motor rotation, drives sampler holder 3-2 to vacillate now to the left, now to the right; At the end of waving, described sampler holder 3-2 is swung to vertical direction;
A-3. described Micro-processor MCV reads the described reaction cup pallet 5-9 vertical motion positional information that described interlock position detecting circuit detects, calculates it and waits for that reaction cup puts into position difference, determining drive motor rotation direction and speed; Under described Micro-processor MCV controls, described sampling thief displacement presses down control circuit and controls the running of described drive motor, described reaction cup pallet 5-9 is moved to and waits for that reaction cup puts into position, open door simultaneously;
A-4. said process is if there is exception, controls described display circuit display alarm information;
B. thermostatic control: described Micro-processor MCV controls described sampling thief sample automatic detection device internal temperature constant temperature within the steady temperature deviation range preset;
B-1: described temperature sensing circuit reads described sampling thief sample automatic detection device inner air channel 6-4 outlet temperature, judges whether it is within the inner steady temperature deviation range of described sampling thief sample automatic detection device; If not, step B-2 is performed; If so, step C is performed;
B-2: judge whether the mxm. higher than steady temperature deviation range in described machine, if perform step B-3; If not, step B-4 is performed;
B-3: the built-in temperature of sampling thief pattern detection control device described in cycle detection, until it is lower than the mxm. of steady temperature deviation range in described machine, then performs step C;
B-4: start described constant temperature control circuit and control the built-in temperature that heating system heats Posterior circle detects described sampling thief pattern detection control device, until the internal temperature of described sampling thief sample automatic detection device is higher than the minimum of steady temperature deviation range in described machine, stop described constant temperature control circuit work, perform step C;
C. reaction cup inserts detection: described in described Micro-processor MCV cycle detection, whether sampling thief sample automatic detection device puts into reaction cup, until detect that rear execution step D is put in reaction;
D. Detection Information reads: under described Micro-processor MCV controls, and under described sampling thief location presses down coordinated signals unit controls, described drive motor running makes described reaction cup pallet 5-9 vertically be moved upwards up to information load position; Described Micro-processor MCV controls the Detection Information in described Detection Information read module reading reaction cup; Described Detection Information comprises and waves number of times, mode of operation, calibration curve, if mode of operation is calibration mode or matter school pattern, continues to read calibration figure;
E. reaction cup temperature detection: under described Micro-processor MCV controls, described sampling thief location presses down drive motor running described in coordinated signals unit controls, makes described reaction cup pallet 5-9 vertically be moved down into mixing position; The temperature of the described reaction cup that described infrared temperature testing circuit gathers; Described reaction cup temperature is read in described Micro-processor MCV circulation, until it is higher than the temperature of reaction preset, then performs step F;
F. mode of operation judges: if the mode of operation that described Detection Information read module reads is for detecting pattern or matter school pattern, perform step G, if be calibration mode, and execution step J;
G precompressed: under described Micro-processor MCV controls, described sampling thief location presses down drive motor running described in coordinated signals unit controls, band dynamic pressure plate 2-18 vertically moves downward, and by described sampling thief sample top vertically to pressing to precompressed position, then turns back to mixing position; Described mixing and light path positioning unit control to mix motor rotation, described sampling thief 8 being vacillated now to the left, now to the right according to waving number of times in described Detection Information together with reaction cup, resting on vertical position at the end of waving;
H. once press down: under described Micro-processor MCV controls, described sampling thief location presses down drive motor running described in coordinated signals unit controls, band dynamic pressure plate 2-18 vertically moves downward, and by described sampling thief sample top vertically to pressing to a depressed position, then turns back to mixing position; A described depressed position is lower than described precompressed position; Described mixing and light path positioning unit control described mixing motor rotation, described sampling thief 8 being vacillated now to the left, now to the right according to waving number of times in described Detection Information together with reaction cup, resting on vertical position at the end of waving;
I. press down for two times: under described Micro-processor MCV controls, described sampling thief location presses down the running of coordinated signals unit controls drive motor, band dynamic pressure plate 2-18 vertically moves downward, and by described sampling thief 8 top vertically to pressing to secondary depressed position, then turns back to mixing position; Described secondary depressed position is lower than a described depressed position; Described mixing and light path positioning unit control described mixing motor rotation, described sampling thief 8 being vacillated now to the left, now to the right according to waving number of times in described Detection Information together with reaction cup, resting on vertical position at the end of waving;
J. detect and show with result: described Micro-processor MCV reads in the detected signal value that described signal gathering unit exports, calculate in conjunction with described calibration curve and detect concentration of specimens, control described display circuit display testing result; By described bluetooth module, testing result is uploaded to flat board/mobile phone;
L. calibrate adjustment: if mode of operation is calibration mode, according to the difference of testing result and calibration figure, adjust the enlargement factor of described signal gathering unit, then perform step M; Otherwise, directly perform step M;
M. matter school process: if mode of operation is matter school pattern, compare testing result and calibration figure, if there are differences, according to calibration curve after calibration figure and testing result calculating matter school, and preserves, then performs step N; Otherwise, directly perform step N;
N. reaction cup is exited: described Micro-processor MCV controls mixing and drives described mixing motor that sampler holder 3-2 is swung to vertical state with light path conversion unit, control described sampling thief location again to press down coordinated signals unit controls drive motor and drive and press down tooth bar 2-1 and be vertically moved upwards up to out bin location, return step C.
CN201510082701.9A 2015-02-15 2015-02-15 Control system and control method of integrated quantitative sampling reagent adding device detector Pending CN104678116A (en)

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