CN104664052B - Functional living being feed production technology - Google Patents
Functional living being feed production technology Download PDFInfo
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Abstract
The present invention is that the feedstuffs such as corn, dregs of beans are carried out into Partial digestion, wherein 1/3 albumen is digested into small peptide, more than half starch is degraded to the monose such as fructose, glucose, and small peptide, monose can directly be absorbed by animal body.Resistance factor urase, pectin, phytic acid, cellulose reduce 70% or so respectively in the feed of preparation, soybean protein sensitizing factor 7S, 11S globulin reduce by 50% or so, it is suitable as the opening material and creep feed of young animal, aquaculture bait and the source of nutrition of pregnancy period and suckling animals.Biological feedstuff prepared by the present invention, which has, easily to be absorbed, and the advantages of reducing animal diarrhea rate and improve palatability, simple production process, small investment, cost is low, suitable for large-scale production and popularization.
Description
Technical field
Patent of the present invention is related to a kind of new type functional biological feedstuff preparation method, belongs to field of animal feed.
Background technology
Protein is mainly vegetable protein in feed at present, and molecular weight is big, generally it is tens of thousands of between hundreds of thousands, be not easy by
The digested organ of animal body is digested and assimilated completely, and the digestive organs of especially poult poultry are not yet sound, digest and assimilate protein more
Difficulty, diarrhoea is produced as 7S, 11S globulin in dregs of beans albumen easily cause swine alimentary canal allergic reaction（Yao Kun etc., 2011；
Liu Haiyan etc. 2011；Old middle equality 2011）；Aquiculture animal alimentary canal is shorter than mammal, and protein is digested and assimilated
More not exclusively, the latter is most of as excrement excretes, and has not only wasted resource but also has polluted water body.Therefore, it is necessary in vitro
Partial digestion is carried out to the nutriment in raising to promote digesting and assimilating for nutriment.
In feed in addition to containing nutriments such as protein, starch, often also contain for example various hemicelluloses of SNSP
Element（Xylan, beta glucan, mannosan）, phytic acid, the resistance factor such as pectin increase intestinal contents viscosity, influence animal pair
Feed is mesotrophic to be digested and assimilated.Many scholar's research add absorption of the exogenous enzymes increase animal to nutrition in feed.He Wan
Neck etc.（Crude fibre complex enzyme is to meat chicken production performance, influence [J] the China grain and oil of digestive enzyme activity agent immunologic function
Report, 2010,25 (8)：65-69）The production performance of AA broiler chicken is promoted after addition crude cellulase in AA daily ration of broiler,
Feedstuff-meat ratio is reduced, and improves digestive enzyme activity in alimentary canal and serum.In addition, in recent years Microbe Fermented Soybean Meal also into
The study hotspot of numerous scholars.Wu Shenghua etc.（More thalline solid fermentation dregs of beans produce small peptide feed [J] food and fermentation industries,
2008,34 (10)：113-115）With saccharomycetes to make fermentation dregs of beans, trypsin ihhibitor degradation rate is up to 56.2% in dregs of beans, small peptide
Content improves 3-4 times.Old middle equality（Research [J] the China herding magazine of aspergillus oryzae fermented bean dregs trophism, 2011,47
（9）：40-44）Dregs of beans has been handled using aspergillus oryzae solid state fermentation, has reduced that big, middle molecule protein matter is horizontal, improve small peptide and
The level of free amino acid, the ANFs and antigen protein of dregs of beans are effectively eliminated, have improved the nutritive value of dregs of beans,
But amount of substrate, total protein after fermenting and 5.14%, 3.06% and 3.86% can be have lost respectively.Song etc.
（Fermentation of soybean meal and its inclusion in diets for weaned pigs
reduced diarrhea and mensures of immunoreacitivity in the plasma[J]. Animal
feed science and technlolgy[J]. 2010, 159: 41-49）Fed with fungi and bacterium combined fermented bean dregs
Piglet, diarrhea rate is reduced, improve feed conversion rate, thus it is speculated that may be reduction of and cause pig to produce immune response in dregs of beans
The reason for macro-molecular protein.
Chinese patent 201410277974.4 discloses the preparation method of a broad sow specific enzyme solution corn Silage,
It is characterized in that step is as follows：1. by the corn ear crushing and beating with coating, it is made and contains corn kernel, coating and corncob
Corn ear mixing slurry；2. by water, molasses, cellulase, zytase, pectase and mixed bacteria liquid according to weight ratio be 100：
1~500：0.1~5：0.05~2.5：0.05~2.5：1~50 mixing, enzymolysis liquid is made, contains lactic acid bacteria in mixed bacteria liquid
Strain liquid, Bacillus subtilis strain liquid and S. cervisiae strain liquid；3. by corn ear mixing slurry, enzymolysis liquid and wheat bran according to
Weight ratio is 100：1~15：2~30 well mixed rear heap buried compactings, carry out anaerobic fermentation, enzymolysis, are made and digest corn silage
Feed.Enzymolysis corn Silage made of it, it is rich in soluble polysaccharide, lactic acid, small peptide, amino acid, vitamin and prebiotic
Bacterium, have the characteristics that amino acid enriches balance, nutrition is high, easy to digest, palatability is good, can significantly improve milk yield of sow, it is bright
Aobvious increase feed intake, and substantially reduce prevention of sow constipation.
Although scholars have carried out numerous studies, also it is seen that shortcoming and deficiency therein：
Although the 1, with the addition of various enzyme preparations in feed, enzyme and substrate-function must have water, in the dry state can not
Carry out enzyme digestion reaction.Typically there was only short 3-4h to being excreted with excrement after animal eats feed, therefore can not fill
The effect of external enzyme is waved in distribution.
2nd, in general enzyme digestion reaction under the conditions of liquid（Concentration of substrate 10% or so）Generally requiring 4-8h could be substantially complete
Into, and solid phase digests（Water content 50% or so）6-10h is needed to can be only achieved enzyme digestion reaction balance.
The 3rd, although Microbe Fermented Soybean Meal energy Partial digestion soybean protein becomes polypeptide and small peptide, and the part nutrition that can degrade
Resistance factor, but also there is distinct disadvantage.
（1）Production cycle is grown, the time required to being prepared plus strain, the whole 4-5 days production cycle；
（2）Floor space is big, and fermented bean dregs are generally fermented in cement pit, such as feeds intake daily 1 batch, then needs 4-5
Large-scale cement pond；As fed intake daily 2 batches, then 8-10 large-scale cement pond is needed.Yield is bigger, and cement pit is more；
（3）Labor intensity is big, low production efficiency；
（4）Substrate is consumed during microbial growth, consumed energy, causes the waste of resource.
The content of the invention
For existing process deficiency, the invention provides following technique to be invented；
1st, certain mass corn flour is taken, adds moisture to dissipate, it is 5%-30% to make Maize meal concentration, adjusts the temperature to 50 DEG C -70 DEG C,
PH 4.0-8.0, amylase 10-50 u/g are added, digest 30-10 0min, adjusted to iodine test starch reaction to be negative
PH3.0-7.0, add carbohydrase 50-300 u/g, digest 5-24h, slag-liquid separation, slag is germ protein and maize peel, is merged into 2
Powder carries out solid phase enzymolysis, and liquid, which carries out being concentrated into solid concentration, reaches 30%-60%, as fructose syrup.
2nd, take with 1 equivalent corn flour and bean cake powder, add the slag charge separated in 1 and be well mixed.
3rd, pure water is taken, quality is 2 mixed powders（Without slag charge）The 40%-80% of gross mass, it is equally divided into 2 parts.Its
In 1 part by compound protease（1398 neutral proteinases：2709 alkali protease=1:2-5）Scattered to dissolve in wherein, addition is mixed
The 0.5%-5.0% of material quality is closed, as enzyme liquid 1；Amylase, carbohydrase, pectase, phytase, cellulase are disperseed to be dissolved in
In another water, addition is amylase 10-50 u/g；Carbohydrase 50-300 u/g；Pectase 50-100 g/t；Phytase
50-100 g/t；Cellulase 10-100 g/t, as enzyme liquid 2.
4th, enzyme liquid in 31,2 is uniformly sprayed into compound 2, addition solid phase enzymolysis device after fully stirring evenly, 30-50 DEG C of constant temperature,
It is closed, mix slowly enzymolysis 6-10 h.
5th, enzymolysis compound is dried, the fructose syrup obtained in 1, drying temperature 100- is uniformly added before drying
140 DEG C, get product new function feed.
Biological feedstuff feature and effect prepared by the present invention：
1st, oligomeric peptide content is high.Protein content 20% or so in feed, wherein 1/3 about 7.5% or so is that poultry is directly inhaled
The molecular weight ＜ 2kd of receipts oligopeptide, 1/3 is molecular weight ＞ 2kd polypeptide, can be immature poultry and aquaculture exempt because
Digestive organs are unsound or short and influence the problem absorbed to protein digestibility.
2nd, contents of monosaccharides is high.Starch-containing more than 50% in feed, wherein 25%-30% is the fruit that immature livestock and poultry can directly absorb
Sugar and glucose, mouthfeel is compared with sweet tea.
3rd, palatability is good.U.S. drawing occurs under 110 DEG C of -140 DEG C of instantaneous high-temperatures of pneumatic conveying drying for monose and oligopeptide in feed
Moral is reacted, and produces special fragrant and sweet smell and mouthfeel, palatability is especially good, can increase animal feed intake.
4th, the nutrition resistance factor is low.Product after measured, urase, the trypsin-resistant factor, pectin, phytic acid, cellulose and
7S and 11S contents in glycinin greatly improve feed absorption rate, reduce material than reducing by 70% or so before enzymolysis
Meat ratio.
5th, suitable for poult opening material and creep feed, as poult poultry monthly age increase gradually decreases use ratio, can make
Poult poultry safety spend stress the phase, and be greatly reduced caused by 7S and 11S dregs of beans globulin intestinal intolerance reaction and caused by
Diarrhoea.
6th, yield is big, can be digested with 24h continuous productions and realized on-line automatic control, a production line output is up to annual output
It is thousands of to more than 100,000 tons.
Brief description of the drawings
Fig. 1：Produce the flow sheet of 20,000 tons of new type functional feeds per year
Embodiment
The present invention is further detailed example below, but the invention is not restricted to these experimental examples.
The laboratory preparation technology of the new type functional feed of embodiment 1
（1）The mesh corn flour 100g of 40 mesh -60 is taken, adds water to 1000ml, it is 10% to make concentration of substrate, is warming up to 50 DEG C, is adjusted
PH5.0, amylase 700u is added, stirring enzymolysis 40min, starch negative is examined to iodine test（Brown）, 55 DEG C of temperature regulating,
PH4.0, carbohydrase 3500u, stirring saccharification 6h are added, centrifugation carries out slag-liquid separation, and liquid is rotated to be concentrated by evaporation to solid content
35% or so, as fructose syrup, slag specimen（Maize germ and maize peel）Separate standby.
（2）The mesh corn flour 100g of 40 mesh -60, the mesh bean cake powder 90g of 40 mesh -60 is taken to be well mixed, reenter（1）Middle slag mixing is equal
It is even.
（3）Fetch water 76ml, is divided into two parts, takes compound protease（1398 neutral proteinases：2709 alkali proteases=
1:2-5）0.36 g dispersing and dissolvings are in wherein 1 part of water, as enzyme liquid 1；Take amylase 700u, carbohydrase 3500u, pectase
9.5mg, phytase 9.5mg, cellulase 5mg are scattered to be dissolved in another water, as enzyme liquid 2.
（4）Enzyme liquid in 31,2 is sprayed into compound under agitation（2）In, it is thoroughly mixed uniformly, is put into self-control solid phase
In enzymolysis device, 40 DEG C of constant temperature stirring enzyme digestion reaction 6h.
（5）Take out（4）Zymolyte, spray under agitation（1）Middle fructose syrup, 110 DEG C -120 DEG C are put into after well mixed
Dried in constant temperature blast drying oven, it is new function feed to crush.
The new function feed nutrient of embodiment 2 is analyzed
To carrying out Analysis of Nutritive Composition in obtained new function feed in embodiment 1, peptide content measure refers to GB/T
22492-2008《Soy peptide powder》The assay method of middle peptide content, peptide content 6.67%；Content of reducing sugar measure refers to GB/T
15038-2006《Grape wine, fruit wine universaling analysis method》In reduced sugar titration Fehling's reagent method, content of reducing sugar is
15.12%；Urease activity measure refers to GB/T 8622-2006《The assay method of urease activity in feed bean product》, work(
Energy type feed urease activity is 0.035U/g, and 76.67% is have dropped compared with control group；Pectin measure is with reference to NY 82.11-1988 fruits
The measure of juice assay method-pectin, pectin content is 3.85% in functional feed, and 70.63% is reduced compared with control group；Phytic acid
Measure uses ferric trichloride titration（Xiao Chuanhao, it is old come into it is fast and convenient measure phytic acid method [J] chemical industry when print, 24
（8）：66-68.）, phytic acid content is 0.40% in functional feed, and 65.85% is reduced compared with control group；Fiber content measure reference
GB/T 6434-2006《Coarse-fibred assay in feed》, crude fiber content is 1.89% in functional feed, compared with control group
Reduce 66.25%；Using soy proteinaceous sensitizing factor 7S, 11S globulin in ELISA brake feed, compared with
Control group reduces by 69.32%.
The pilot plant production process of the new type functional feed of embodiment 3
（1）40 mesh corn flour 100kg are taken, are put into reactor, add the kg of water 660, it is 15% to make concentration of substrate, is warming up to 55
DEG C, pH6.0 is adjusted, adds the u of amylase 1,400,000,60 min of stirring enzymolysis or so, starch negative is examined to iodine test（Brown）, adjustment
Temperature 60 C, pH4.5, the u of carbohydrase 7,000,000 is added, stirring enzymolysis 10h, is centrifuged, liquid is concentrated into through decompression evaporator to be contained admittedly
Amount 45%, i.e. fructose syrup, solid phase slag for handling in next step.
（2）40 mesh corn flour 100kg, 40 mesh bean cake powder 100kg are taken to be well mixed, solid phase slag in being blended into 1, mixing is equal
It is even.
（3）Water intaking, quality are the gross mass 50% of corn flour and bean cake powder, i.e. 100kg, are divided into 2 parts, every part of 50kg.
（4）Take compound protease（1398 neutral proteinases：2709 alkali protease=1:2-5）0.64kg is scattered to dissolve in it
In in 1 part of water；Take the u of amylase 1,750,000, the u of carbohydrase 7,000,000, pectase 15g, phytase 15g, cellulase 10g are scattered to dissolve in
In another water.
（5）Two kinds of enzyme liquids are sprayed into compound under agitation（2）In, it is thoroughly mixed uniformly, is put into solid phase enzymolysis device
In 45 DEG C of constant temperature stirring enzymolysis 8h.
（6）Zymolyte enters pneumatic drier, will（1）Middle fructose syrup sprays into hothouse and powder combination drying, rotation
Powder is new function feed in wind collector.
The clinical animal experiment of the biological feedstuff of embodiment 4
Kind, parity and the same or like weanling pig 40 of body condition are chosen, is equally divided into two groups, every group 20, point
For experimental group and blank control group.Control group uses Corn-soybean-fish meal type basal diet, and formula design is with reference to NRC
(1998)；The biological feedstuff that test group is prepared by albumen and the energy level addition present invention, substitutes the jade in basal diet completely
Rice and dregs of beans.The feeding environment of two groups of experimental animals, raise competence, epidemic preventing sanitary condition are identical during experiment, freely adopt
Food and drinking-water, continuous feeding 40 days.Result of the test is as shown in table 1, shows test group of animals feedstuff-meat ratio level and Incidence of Diarrhea
All it is remarkably decreased compared with control group（P ＜ 0.05）.This experiment carries out 3 repetitions.
The Growth Performance of Weaning Piglets of table 1
Table 1 Growth performance of weaned piglets
Above-mentioned detailed description is illustrating for one of them possible embodiments of the present invention, and the embodiment is simultaneously not used to
The scope of the claims of the present invention is limited, all equivalence enforcements or change without departing from carried out by the present invention, is intended to be limited solely by the technology of the present invention
In the range of scheme.
Claims (1)
- A kind of 1. method for producing functional feed, using corn flour and bean cake powder as raw material, it is characterised in that comprise the following steps：A, corn flour adds moisture to dissipate, and adds amylase successively and carbohydrase is digested respectively, slag-liquid separation, liquid phase is steamed Hair concentration obtains fructose syrup, and slag specimen solid phase is maize germ and skin；B, the corn flour and bean cake powder with a equivalent are sufficiently mixed, then compound is well mixed to obtain with solid slag in a；C, the compound protease being made up of 1398 neutral proteinases and 2709 alkali proteases is dissolved in water and obtains enzyme liquid 1；It will form sediment again Powder enzyme, carbohydrase, pectase, phytase, cellulase are separately dissolved in water and obtain enzyme liquid 2；D, enzyme liquid in c 1 and enzyme liquid 2 are sprayed into b compounds under agitation, constant temperature stirring enzymolysis after being well mixed；E, after enzymolysis terminates, gained fructose syrup in a is sprayed under agitation, is dried, is crushed, packing, obtaining biological feedstuff；Wherein, corn flour amount of water is 3-20 times of corn flour quality in a, 50 DEG C -70 DEG C of reaction temperature, pH adjust to 5.0-8.0, amylase addition 10-50 u/g, enzymolysis time 30-100 min to iodine test starch reaction is adjust after negative PH3.0-7.0, carbohydrase 50-300u/g is added, digest 5-24 h, then carry out slag-liquid separation, liquid phase, which is evaporated, to be concentrated into Solid content reaches 30%-60%, as fructose syrup, and solid phase slag specimen is maize germ and skin；Corn flour and bean cake powder mass ratio are 1.0-1.5 in the b:1.0；Water addition described in the c is corn flour and the 40%-80% of bean cake powder mixture quality；Water intaking is divided into 2 parts, Wherein 1 part plus compound protease, 1398 neutral proteinases：2709 alkali protease=1:2-5, fully dissolving, addition is corn The 0.5%-5.0% of powder and bean cake powder gross mass；Amylase, carbohydrase, pectase, phytase, cellulose are added in another water Enzyme, dosage are respectively amylase 10-50 u/g, carbohydrase 50-300 u/g, pectase 50-150g/t, phytase 50-150 g/ T, cellulase 10-100g/t；The enzyme liquid in c is sprayed into b compounds under agitation in the d, is put into after well mixed in solid phase enzymolysis device, 30 DEG C -55 DEG C of constant temperature mix slowly enzymolysis 6-10h；Drying temperature is 100-140 DEG C in the e, before it is dried uniform penetrating fructose syrup, fructose syrup amount content in finished product For 15.0%-40.0%.
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| CN106212944A (en) * | 2016-08-03 | 2016-12-14 | 日照普惠动物营养科技有限公司 | A kind of Weanling pig compound feed containing low antigen fermented bean cake and preparation method |
| CN108094709A (en) * | 2017-12-22 | 2018-06-01 | 浙江格蕾斯生物科技有限公司 | The processing method of new animal feed based on plant residue |
| CN110200131A (en) * | 2019-07-01 | 2019-09-06 | 博益德（北京）生物科技有限公司 | A kind of single enzymatic hydrolysis mixed fermentation prepares the method and application of functional fiber feed |
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| CN1201830A (en) * | 1998-06-19 | 1998-12-16 | 陈远德 | Compounded saccharifying enzyme |
| CN1206933C (en) * | 2003-04-01 | 2005-06-22 | 朱德高 | Method of processing animal feed using enzymatic hydrolysis |
| CN101530162B (en) * | 2008-03-10 | 2011-11-23 | 北京大北农科技集团股份有限公司 | Preparation method of novel protein feed additive |
| CN101548717B (en) * | 2009-04-21 | 2013-04-17 | 青岛蔚蓝生物集团有限公司 | Feeding solid complex enzyme and preparation method thereof |
| CN102132770B (en) * | 2011-02-18 | 2013-04-24 | 青岛根源生物技术集团有限公司 | Corn bean pulp type feed complex enzyme additive and application thereof |
| CN102763768A (en) * | 2012-08-03 | 2012-11-07 | 山东和实集团有限公司 | Production process of fermented soybean meal by synchronous solid fermentation and enzymolysis |
| CN103734481B (en) * | 2013-12-23 | 2014-12-10 | 湖南鸿鹰生物科技有限公司 | Feed compound enzyme containing feeding complex enzyme and preparation method of feed compound enzyme |
| CN103734480B (en) * | 2013-12-23 | 2015-04-15 | 湖南鸿鹰生物科技有限公司 | Feed compound enzyme capable of improving animal appetite and preparation method thereof |
| CN104054964A (en) * | 2014-06-20 | 2014-09-24 | 安徽农业大学 | Method for preparing special enzymolysis corn silage for sows |
| CN104012775B (en) * | 2014-06-27 | 2016-01-13 | 成都美溢德生物技术有限公司 | A kind of method of hydrolyzed vegetable protein |
| CN104171667B (en) * | 2014-07-30 | 2016-06-29 | 樊继绪 | For the compound chicken feed that laying period laying hen is special |
| CN106615663A (en) * | 2014-08-26 | 2017-05-10 | 威海市世代海洋生物科技有限公司 | Seaweed biological feed and preparation method thereof |
| CN104905073A (en) * | 2015-06-30 | 2015-09-16 | 河南双成生物科技有限公司 | Method for producing meat duck complete feed by solid state enzyme solution and fermentation |
| CN106035990B (en) * | 2016-07-15 | 2019-12-17 | 重庆市畜牧科学院 | Method for preparing biological feed by solid-state enzymolysis fermentation of citrus pulp, product and application thereof |
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Effective date of registration: 20210615 Address after: Jiangsu Sanyi Animal Nutrition Technology Co., Ltd., 318 Binhu Avenue, Pizhou City, Xuzhou City, Jiangsu Province, 221300 Patentee after: JIANGSU SANYI ANIMAL NUTRITION TECHNOLOGY Co.,Ltd. Patentee after: JIANGSU SANYI BIOENGINEERING Co.,Ltd. Address before: Jiangsu Sanyi Animal Nutrition Technology Co., Ltd., 318 Binhu Avenue, Pizhou City, Xuzhou City, Jiangsu Province, 221300 Patentee before: JIANGSU SANYI ANIMAL NUTRITION TECHNOLOGY Co.,Ltd. |
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