CN104664050A - Microbiological feed for holothurian culture - Google Patents
Microbiological feed for holothurian culture Download PDFInfo
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- CN104664050A CN104664050A CN201310639823.4A CN201310639823A CN104664050A CN 104664050 A CN104664050 A CN 104664050A CN 201310639823 A CN201310639823 A CN 201310639823A CN 104664050 A CN104664050 A CN 104664050A
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- bacterial classification
- saccharomyces cerevisiae
- microbiological feed
- streptococcus lactis
- holothruian cultures
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Abstract
The invention provides a microbiological feed for holothurian culture. The microbiological feed is obtained by preparing strains by adopting bacillus natto, streptococcus lactis, leatobacillus acidophilus and saccharomyces cerevisiae and then mixing with seaweed meal and shell powder. The microbiological feed is prepared from the following components in percentage by weight: 5-25% of bacillus natto, 10-30% of streptococcus lactis, 15-30% of leatobacillus acidophilus, 5-20% of saccharomyces cerevisiae, 5-15% of seaweed meal, and 5-15% of shell powder.
Description
Technical field
The present invention relates to a kind of holothruian cultures microbiological feed and preparation method thereof, belong to the technical field of biological materials that microbial modulator is applied in culture fishery.
Background technology
Sea cucumber has another name called stichopus japonicus, extra large mouse, sea gherkin, extra large eggplant, is a kind of famous and precious aquatic/marine animals, has survived on earth 600,000,000 years, and ancients find " its benefit warm in nature, sufficient enemy's ginseng ", gain the name because of benefiting action.Meat quality of sea cucumber is soft tender, nutritious, typical high protein, low fat food, flavour fat is beautiful, and local flavor is graceful, is the name food delicacies had long enjoyed a good reputation, one of seafood delights " eight delicacies ", equally celebrated for their achievements with bird's nest, abalone, shark's fin, refined taste often plays the role of " axle of presenting a theatrical performance as the last item on a programme ", one of soul being regarded as Chinese meal.
Holothruian cultures, become the pillar industry of the Northeast's aquaculture just gradually.But the research in current China and even world wide in holothruian cultures in nutritional need and feed technology is seriously delayed, holothurian feed form common on market mostly is Powdered and coarse granule shape.Because sea cucumber aquaculture kind alimentary canal is short, digestive enzymes lacks, and conventional feed, after being taken food, can not get fully digesting and assimilating in vivo, cause the analyte of protein to have more than 60% to enter in water with useless nitrogen form, the P elements in mineral matter has about 53% to enter in water with useless phosphorus form.These two kinds of elements are the main causes causing Water Eutrophication, polluted water.
Summary of the invention
The object of the present invention is to provide a kind of holothruian cultures microbiological feed and preparation method thereof.The present invention makes full use of the synergy between bacterial classification, then coordinates organic feed, makes feed itself be easy to be absorbed by sea cucumber, and environmental sound.
For achieving the above object, the present invention adopts following technical scheme:
Holothruian cultures microbiological feed, adopt bafillus natto (Bacillus natto), streptococcus lactis (Streptococcus lactis), lactobacillus acidophilus (Leatobacillus acidophilus) and saccharomyces cerevisiae (Saccharomyces cerevisiae) to prepare bacterial classification, and mix with seawood meal and oyster shell whiting; Described feed comprises by mass percentage: bafillus natto 5 ~ 25%, streptococcus lactis 10 ~ 30%, lactobacillus acidophilus 15 ~ 30%, saccharomyces cerevisiae 5 ~ 20%, seawood meal 5 ~ 15%, oyster shell whiting 5 ~ 15%.
Holothruian cultures microbiological feed provided by the invention, preferably, described feed comprises by mass percentage: bafillus natto 20%, streptococcus lactis 28%, lactobacillus acidophilus 22%, saccharomyces cerevisiae 16%, seawood meal 8%, oyster shell whiting 6%.
Present invention also offers the preparation method of above-mentioned holothruian cultures microbiological feed, comprise the following steps:
1. inoculate: described each bacterial classification is connected on slant medium; Bafillus natto cultivation temperature 25 ~ 30 DEG C, incubation time is 36 ~ 48h; Lactobacillus acidophilus cultivation temperature is 30 ~ 35 DEG C, and incubation time is 36 ~ 48h; Streptococcus lactis cultivation temperature is 30 ~ 35 DEG C, and incubation time is 36 ~ 48h; Saccharomyces cerevisiae cultivation temperature is 32 ~ 37 DEG C, and incubation time is 36 ~ 48h;
2. Spawn incubation: be inoculated in respectively by described bacterial classification and fill in the 50mL conical flask of fluid nutrient medium, culture medium antiseptic sea water is prepared, and pH is 6.0 ~ 8.0; Conical flask puts into shaking table vibration, and temperature is 27 DEG C, vibration 48 ~ 72h; Employing blood counting chamber counts, and in bacterium liquid, bacterial classification quantity reaches 10 respectively
8individual/mL;
3. one grade fermemtation: be inoculated in respectively by described bacterial classification and fill in the 1000mL conical flask of fluid nutrient medium, culture medium antiseptic sea water is prepared, pH6.0 ~ 8.0, put into shaking table vibration, temperature is 27 DEG C, vibration 48 ~ 72h; Bacterial classification quantity is made to reach 10 respectively
8individual/more than mL, mixes the mass percent of each bacterial classification by described bacterial classification;
4. second order fermentation: mixed bacterium liquid is inoculated in the fermentation tank filling composite bacteria agent capable culture medium, composite bacteria agent capable medium pH 6.0 ~ 8.0, inoculum concentration is 1 ~ 5%, adds seawood meal and oyster shell whiting, cultivate 4 ~ 7 days at 27 DEG C, make bacterium number in zymotic fluid reach 8 ~ 15 × 10
8individual/mL, namely obtains described holothruian cultures microbiological feed.
The preparation method of holothruian cultures microbiological feed provided by the invention, described composite bacteria agent capable culture medium prescription is: glucose 1 ~ 5g/100mL, honey 1 ~ 8g/100mL, sucrose 5 ~ 10g/100mL, MC peptone 1 ~ 5g/100mL, peptone 0.1 ~ 0.8g/100mL, KH
2pO
40.2 ~ 0.5g/100mL, K
2hPO
40.5 ~ 3g/100mL, MgSO
47H
2o0.5 ~ 3g/100mL, NaCl0.5 ~ 10g/100mL, all the other are distilled water.
The preparation method of holothruian cultures microbiological feed provided by the invention, if no special instructions, fluid nutrient medium used is conventional culture medium of the prior art, does not repeat at this.
Compared to prior art, holothruian cultures microbiological feed provided by the invention is after joining breeding water body, and the amount reproduction of microorganism can suppress harmful pathogenic microorganism to a certain extent, and the efficiency that can promote the decomposition of feed and be absorbed by sea cucumber.The organic pollution simultaneously produced by fish in breeding water body by the decomposition of microorganism of the present invention, thus makes water quality be purified, and does not harm environment.
Detailed description of the invention
The following examples can make the present invention of those of ordinary skill in the art's comprehend, but do not limit the present invention in any way.
Embodiment 1
Holothruian cultures microbiological feed, adopts bafillus natto 5%, streptococcus lactis 30%, lactobacillus acidophilus 15%, saccharomyces cerevisiae 20%, seawood meal 15%, oyster shell whiting 15%, prepares according to following steps:
1. inoculate: described each bacterial classification is connected on slant medium; Bafillus natto cultivation temperature 25 ~ 30 DEG C, incubation time is 36 ~ 48h; Lactobacillus acidophilus cultivation temperature is 30 ~ 35 DEG C, and incubation time is 36 ~ 48h; Streptococcus lactis cultivation temperature is 30 ~ 35 DEG C, and incubation time is 36 ~ 48h; Saccharomyces cerevisiae cultivation temperature is 32 ~ 37 DEG C, and incubation time is 36 ~ 48h;
2. Spawn incubation: be inoculated in respectively by described bacterial classification and fill in the 50mL conical flask of fluid nutrient medium, culture medium antiseptic sea water is prepared, and pH is 6.0 ~ 8.0; Conical flask puts into shaking table vibration, and temperature is 27 DEG C, vibration 48 ~ 72h; Employing blood counting chamber counts, and in bacterium liquid, bacterial classification quantity reaches 10 respectively
8individual/mL;
3. one grade fermemtation: be inoculated in respectively by described bacterial classification and fill in the 1000mL conical flask of fluid nutrient medium, culture medium antiseptic sea water is prepared, pH6.0 ~ 8.0, put into shaking table vibration, temperature is 27 DEG C, vibration 48 ~ 72h; Bacterial classification quantity is made to reach 10 respectively
8individual/more than mL, mixes the mass percent of each bacterial classification by described bacterial classification;
4. second order fermentation: be inoculated in the fermentation tank filling composite bacteria agent capable culture medium by mixed bacterium liquid, composite bacteria agent capable medium pH 6.0 ~ 8.0, inoculum concentration is 1 ~ 5%, adds seawood meal and oyster shell whiting, cultivates 7 days at 27 DEG C, makes bacterium number in zymotic fluid reach 12 × 10
8individual/mL, namely obtains described holothruian cultures microbiological feed.
The preparation method of holothruian cultures microbiological feed provided by the invention, described composite bacteria agent capable culture medium prescription is: glucose 1 ~ 5g/100mL, honey 1 ~ 8g/100mL, sucrose 5 ~ 10g/100mL, MC peptone 1 ~ 5g/100mL, peptone 0.1 ~ 0.8g/100mL, KH
2pO
40.2 ~ 0.5g/100mL, K
2hPO
40.5 ~ 3g/100mL, MgSO
47H
2o0.5 ~ 3g/100mL, NaCl0.5 ~ 10g/100mL, all the other are distilled water.
Embodiment 2
Holothruian cultures microbiological feed, adopts bafillus natto 25%, streptococcus lactis 15%, lactobacillus acidophilus 20%, saccharomyces cerevisiae 20%, seawood meal 10%, oyster shell whiting 10%, by method preparation described in embodiment 1, namely obtains described holothruian cultures microbiological feed.
Embodiment 3
Holothruian cultures microbiological feed, adopts bafillus natto 20%, streptococcus lactis 28%, lactobacillus acidophilus 22%, saccharomyces cerevisiae 16%, seawood meal 8%, oyster shell whiting 6%, by method preparation described in embodiment 1, namely obtains described holothruian cultures microbiological feed.
Claims (4)
1. holothruian cultures microbiological feed, it is characterized in that: adopt bafillus natto (Bacillus natto), streptococcus lactis (Streptococcus lactis), lactobacillus acidophilus (Leatobacillus acidophilus) and saccharomyces cerevisiae (Saccharomyces cerevisiae) to prepare bacterial classification, and mix with seawood meal and oyster shell whiting; Described feed comprises by mass percentage: bafillus natto 5 ~ 25%, streptococcus lactis 10 ~ 30%, lactobacillus acidophilus 15 ~ 30%, saccharomyces cerevisiae 5 ~ 20%, seawood meal 5 ~ 15%, oyster shell whiting 5 ~ 15%.
2. holothruian cultures microbiological feed according to claim 1, is characterized in that: described feed comprises by mass percentage: bafillus natto 20%, streptococcus lactis 28%, lactobacillus acidophilus 22%, saccharomyces cerevisiae 16%, seawood meal 8%, oyster shell whiting 6%.
3. the preparation method of holothruian cultures microbiological feed according to claim 1, is characterized in that:
1. inoculate: described each bacterial classification is connected on slant medium; Bafillus natto cultivation temperature 25 ~ 30 DEG C, incubation time is 36 ~ 48h; Lactobacillus acidophilus cultivation temperature is 30 ~ 35 DEG C, and incubation time is 36 ~ 48h; Streptococcus lactis cultivation temperature is 30 ~ 35 DEG C, and incubation time is 36 ~ 48h; Saccharomyces cerevisiae cultivation temperature is 32 ~ 37 DEG C, and incubation time is 36 ~ 48h;
2. Spawn incubation: be inoculated in respectively by described bacterial classification and fill in the 50mL conical flask of fluid nutrient medium, culture medium antiseptic sea water is prepared, and pH is 6.0 ~ 8.0; Conical flask puts into shaking table vibration, and temperature is 27 DEG C, vibration 48 ~ 72h; Employing blood counting chamber counts, and in bacterium liquid, bacterial classification quantity reaches 10 respectively
8individual/mL;
3. one grade fermemtation: be inoculated in respectively by described bacterial classification and fill in the 1000mL conical flask of fluid nutrient medium, culture medium antiseptic sea water is prepared, pH6.0 ~ 8.0, put into shaking table vibration, temperature is 27 DEG C, vibration 48 ~ 72h; Bacterial classification quantity is made to reach 10 respectively
8individual/more than mL, mixes the mass percent of each bacterial classification by described bacterial classification;
4. second order fermentation: mixed bacterium liquid is inoculated in the fermentation tank filling composite bacteria agent capable culture medium, composite bacteria agent capable medium pH 6.0 ~ 8.0, inoculum concentration is 1 ~ 5%, adds seawood meal and oyster shell whiting, cultivate 4 ~ 7 days at 27 DEG C, make bacterium number in zymotic fluid reach 8 ~ 15 × 10
8individual/mL, namely obtains described holothruian cultures microbiological feed.
4. the preparation method of holothruian cultures microbiological feed according to claim 3, is characterized in that: described composite bacteria agent capable culture medium prescription is: glucose 1 ~ 5g/100mL, honey 1 ~ 8g/100mL, sucrose 5 ~ 10g/100mL, MC peptone 1 ~ 5g/100mL, peptone 0.1 ~ 0.8g/100mL, KH
2pO
40.2 ~ 0.5g/100mL, K
2hPO
40.5 ~ 3g/100mL, MgSO
47H
2o0.5 ~ 3g/100mL, NaCl0.5 ~ 10g/100mL, all the other are distilled water.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310639823.4A CN104664050A (en) | 2013-11-29 | 2013-11-29 | Microbiological feed for holothurian culture |
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|---|---|---|---|
| CN201310639823.4A CN104664050A (en) | 2013-11-29 | 2013-11-29 | Microbiological feed for holothurian culture |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105961832A (en) * | 2016-05-05 | 2016-09-28 | 青岛农业大学 | Sea cucumber feed formula containing giant kelp |
| CN106332814A (en) * | 2016-08-19 | 2017-01-18 | 青岛润物源生物科技有限公司 | Fully-ecological holothurian cultivation method |
| CN107183406A (en) * | 2017-05-16 | 2017-09-22 | 山东省海洋生物研究院 | A kind of microorganism live bacteria bait and its production method for shellfish hatchery |
| CN107319207A (en) * | 2017-08-07 | 2017-11-07 | 青岛科亮生物工程有限公司 | A kind of sea cucumber bait of purifying sea water breeding water body |
| CN108157668A (en) * | 2017-11-17 | 2018-06-15 | 辽宁景良生物科技开发有限公司 | A kind of used in mariculture probiotics and its preparation method and application |
-
2013
- 2013-11-29 CN CN201310639823.4A patent/CN104664050A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105961832A (en) * | 2016-05-05 | 2016-09-28 | 青岛农业大学 | Sea cucumber feed formula containing giant kelp |
| CN106332814A (en) * | 2016-08-19 | 2017-01-18 | 青岛润物源生物科技有限公司 | Fully-ecological holothurian cultivation method |
| CN107183406A (en) * | 2017-05-16 | 2017-09-22 | 山东省海洋生物研究院 | A kind of microorganism live bacteria bait and its production method for shellfish hatchery |
| CN107319207A (en) * | 2017-08-07 | 2017-11-07 | 青岛科亮生物工程有限公司 | A kind of sea cucumber bait of purifying sea water breeding water body |
| CN108157668A (en) * | 2017-11-17 | 2018-06-15 | 辽宁景良生物科技开发有限公司 | A kind of used in mariculture probiotics and its preparation method and application |
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Application publication date: 20150603 |