CN104609977B - A kind of biological organic fertilizer containing bacillus subtilis of utilization sugar refinery discarded object - Google Patents
A kind of biological organic fertilizer containing bacillus subtilis of utilization sugar refinery discarded object Download PDFInfo
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- CN104609977B CN104609977B CN201510057269.8A CN201510057269A CN104609977B CN 104609977 B CN104609977 B CN 104609977B CN 201510057269 A CN201510057269 A CN 201510057269A CN 104609977 B CN104609977 B CN 104609977B
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- 235000000346 sugar Nutrition 0.000 title claims abstract description 69
- 239000003895 organic fertilizer Substances 0.000 title claims abstract description 37
- 244000063299 Bacillus subtilis Species 0.000 title claims abstract description 28
- 235000014469 Bacillus subtilis Nutrition 0.000 title claims abstract description 28
- 238000000855 fermentation Methods 0.000 claims abstract description 142
- 230000004151 fermentation Effects 0.000 claims abstract description 142
- 239000000843 powder Substances 0.000 claims abstract description 57
- 238000002360 preparation method Methods 0.000 claims abstract description 16
- 239000012752 auxiliary agent Substances 0.000 claims abstract description 12
- 230000008635 plant growth Effects 0.000 claims abstract description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 47
- 150000001875 compounds Chemical class 0.000 claims description 45
- 239000007788 liquid Substances 0.000 claims description 40
- 238000003756 stirring Methods 0.000 claims description 36
- 238000000034 method Methods 0.000 claims description 30
- 239000012530 fluid Substances 0.000 claims description 26
- 239000003337 fertilizer Substances 0.000 claims description 24
- 235000013379 molasses Nutrition 0.000 claims description 23
- 241000609240 Ambelania acida Species 0.000 claims description 22
- 239000010905 bagasse Substances 0.000 claims description 22
- 240000000111 Saccharum officinarum Species 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 239000000203 mixture Substances 0.000 claims description 18
- 241000894006 Bacteria Species 0.000 claims description 17
- 235000007201 Saccharum officinarum Nutrition 0.000 claims description 17
- 241000196324 Embryophyta Species 0.000 claims description 16
- 239000007787 solid Substances 0.000 claims description 16
- 241000222178 Candida tropicalis Species 0.000 claims description 13
- 241000863387 Cellvibrio Species 0.000 claims description 13
- 238000001556 precipitation Methods 0.000 claims description 13
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 claims description 12
- 241001655322 Streptomycetales Species 0.000 claims description 11
- 229930002875 chlorophyll Natural products 0.000 claims description 11
- 235000019804 chlorophyll Nutrition 0.000 claims description 11
- 230000000740 bleeding effect Effects 0.000 claims description 6
- 239000002270 dispersing agent Substances 0.000 claims description 6
- 238000009736 wetting Methods 0.000 claims description 6
- 238000004806 packaging method and process Methods 0.000 claims description 5
- 241000726221 Gemma Species 0.000 claims description 4
- 230000000149 penetrating effect Effects 0.000 claims description 4
- 239000000344 soap Substances 0.000 claims description 4
- 229920005551 calcium lignosulfonate Polymers 0.000 claims description 3
- RYAGRZNBULDMBW-UHFFFAOYSA-L calcium;3-(2-hydroxy-3-methoxyphenyl)-2-[2-methoxy-4-(3-sulfonatopropyl)phenoxy]propane-1-sulfonate Chemical compound [Ca+2].COC1=CC=CC(CC(CS([O-])(=O)=O)OC=2C(=CC(CCCS([O-])(=O)=O)=CC=2)OC)=C1O RYAGRZNBULDMBW-UHFFFAOYSA-L 0.000 claims description 3
- YDEXUEFDPVHGHE-GGMCWBHBSA-L disodium;(2r)-3-(2-hydroxy-3-methoxyphenyl)-2-[2-methoxy-4-(3-sulfonatopropyl)phenoxy]propane-1-sulfonate Chemical compound [Na+].[Na+].COC1=CC=CC(C[C@H](CS([O-])(=O)=O)OC=2C(=CC(CCCS([O-])(=O)=O)=CC=2)OC)=C1O YDEXUEFDPVHGHE-GGMCWBHBSA-L 0.000 claims description 3
- MOTZDAYCYVMXPC-UHFFFAOYSA-N dodecyl hydrogen sulfate Chemical compound CCCCCCCCCCCCOS(O)(=O)=O MOTZDAYCYVMXPC-UHFFFAOYSA-N 0.000 claims description 3
- 229940043264 dodecyl sulfate Drugs 0.000 claims description 3
- 230000000052 comparative effect Effects 0.000 description 10
- 241000194108 Bacillus licheniformis Species 0.000 description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 6
- 229930006000 Sucrose Natural products 0.000 description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 6
- 229960004793 sucrose Drugs 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 239000007921 spray Substances 0.000 description 5
- 239000002699 waste material Substances 0.000 description 5
- 241000193830 Bacillus <bacterium> Species 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 239000005720 sucrose Substances 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 230000007547 defect Effects 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 description 1
- 241000187362 Actinomadura Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 229920001732 Lignosulfonate Polymers 0.000 description 1
- 235000019082 Osmanthus Nutrition 0.000 description 1
- 241000333181 Osmanthus Species 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 125000002252 acyl group Chemical group 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 229930002868 chlorophyll a Natural products 0.000 description 1
- 238000009264 composting Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 235000021552 granulated sugar Nutrition 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 235000012907 honey Nutrition 0.000 description 1
- 239000004021 humic acid Substances 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000003801 milling Methods 0.000 description 1
- 235000013923 monosodium glutamate Nutrition 0.000 description 1
- 235000018343 nutrient deficiency Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 229940073490 sodium glutamate Drugs 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Botany (AREA)
- Environmental & Geological Engineering (AREA)
- Pest Control & Pesticides (AREA)
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a kind of biological organic fertilizer containing bacillus subtilis of utilization sugar refinery discarded object, the biological organic fertilizer includes sugar refinery discarded object fermentation powder 40 90%, bacillus subtilis powder 5 40%, auxiliary agent 5 20%.In addition, the invention further relates to the preparation method of above-mentioned biological organic fertilizer and its purposes for being used to improve plant growth proterties.
Description
Technical field
The present invention relates to fertilizer field, and in particular to a kind of biology containing bacillus subtilis of utilization sugar refinery discarded object has
Machine fertilizer and its production and use.
Background technology
Sugar industry is one and involves a wide range of knowledge and industry of significant impact.Sugar industry also can while granulated sugar is produced
Substantial amounts of accessory substance and discarded object are produced, such as in cane sugar manufacture technique, substantial amounts of bagasse, filter mud and molasses can be produced
(blackstrap).Bagasse contains substantial amounts of cellulose, containing a large amount of organic and inorganic non-saccharide packets point in filter mud, then contains in molasses
Substantial amounts of sucrose etc., its sugar content is high.The irrational utilization of discarded object is not only the waste to natural resources, and can be right
Environment build-up of pressure.
The application for a patent for invention of application number 97112080 discloses a kind of organic multiple using molasses alcohol waste liquid production activity
Method is expected in Hefei, and its technique carries out high temperature to be first well mixed bagasse, alcohol effluent, filter mud, sludge weight percent
Fermentation；Fermentation is adjusted again；Then composting fermentation；It is finally edaphon fermentation.The invention is solid fermentation, may
In the presence of the halfway potential risk that ferments, inventive method needs are by repeatedly fermentation in addition, and technique is complex.
The application for a patent for invention of application number 200810088386 discloses a kind of using the biological blade face of waste molasses of sugar plant production
The method of fertilizer, concretely comprises the following steps：Round boiling water sterilizing；Blackstrap boiling sterilization, treat its temperature drop to 35 DEG C with
Microbe inoculation strain when lower, and then fermented, untill dense aroma is produced.First, the method uses blackstrap
Used as fermentation raw material, the nutrient needed for ensure that fermenting agent propagation, but this there is also a very big hidden danger, due to sugar
Contain substantial amounts of sucrose in honey, if foliar fertilizer fermentation is incomplete, then in subsequent applications, the sucrose of residual will be attached to
Blade surface, influences the photosynthesis of plant leaf blade, and then influence growing for whole plant.On the other hand, the fermentation side
The foliar fertilizer that method is produced is Liquid Fertilizer, and it has inconvenience in packaging and transit link.
The application for a patent for invention of application number 201410006021 discloses a kind of utilization sugar refinery, including with regard to frequent discarded object
The humic acid type medicine fertilizer composition of microbial bactericide, discarded object therein selects alcohol waste liquid for sugar refinery, and handling process is acidifying
Rather than microbial fermentation.The technique is present to the incomplete defect of sugar refinery waste utilization, and the fertilizer of production is crop base manure,
Crop utilization rate is relatively low, and this application disclose only the effect of increasing income of medicine fertilizer to corn crop of its preparation,
With certain application limitation.
In a word, in the prior art to sugar refinery discarded object utilization and use it for there is difference during crop fertilizer
The defect of degree, for example ferment not thorough, complex process, influence growth and development of plants, packed and transported inconvenience and application limitation
Property etc..
The content of the invention
To solve the problems, such as prior art, the present invention is by sugar refinery discarded object bagasse, filter mud and molasses with specific ratio
Example mixing, is fermented with specifically fermentation microbial inoculum, and fermentation solid powder, and then and bacillus subtilis are obtained by concentrate drying
And corresponding additive is prepared by mixing into solid-state foliar fertilizer.The present invention can either overcome prior art in the defect of the aspects such as fermentation,
And further widened the application of foliar fertilizer.
On the one hand, it is described the present invention relates to a kind of biological organic fertilizer containing bacillus subtilis of utilization sugar refinery discarded object
Biological organic fertilizer includes following component and its weight content：
Sugar refinery discarded object fermentation powder 40-90%
Bacillus subtilis powder 5-40%
Auxiliary agent 5-20%.
The auxiliary agent includes wetting dispersing agent and bleeding agent, wherein,
Described wetting dispersing agent is selected from calcium lignosulfonate, N- sodium lauroyl glutamates, sldium lauryl sulfate
One or more, its weight content accounts for the 2.5-10% of biological organic fertilizer total amount；
Described bleeding agent is selected from one or more in soap powder, sodium lignin sulfonate, penetrating agent JFC, its weight content
Account for the 2.5-10% of biological organic fertilizer total amount.
Preferably, the biological organic fertilizer is solid-state foliar fertilizer, and the biological organic fertilizer includes following component and its weight
Amount content：
Sugar refinery discarded object fermentation powder 60-80%
Bacillus subtilis powder 10-30%
Auxiliary agent 6-15%.
The auxiliary agent includes wetting dispersing agent and bleeding agent, wherein,
Described wetting dispersing agent is selected from calcium lignosulfonate, N- sodium lauroyl glutamates, sldium lauryl sulfate
One or more, its weight content accounts for the 3-7.5% of biological organic fertilizer total amount；
Described bleeding agent is selected from one or more in soap powder, sodium lignin sulfonate, penetrating agent JFC, its weight content
Account for the 3-7.5% of biological organic fertilizer total amount.
It is furthermore preferred that the biological organic fertilizer be solid-state foliar fertilizer, and the biological organic fertilizer include following component and its
Weight content：
Or, the biological organic fertilizer is solid-state foliar fertilizer, and the biological organic fertilizer includes following component and its weight
Content：
Wherein, sugar refinery discarded object fermentation powder of the present invention is obtained by the following method：
Step 1：Sugar refinery discarded object compound is prepared, the compound is mixed to get by bagasse, filter mud and molasses, and its is heavy
Amount is than being 2-8: 2-8: 1-4；
Step 2：The compound that step 1 is obtained is added in fermentation tank, is subsequently adding the about 50%- in terms of compound weight
150% water, and stir, obtain Preliminary fermentation liquid；
Step 3：To the mixed fermentation bacterium that the about 1-10% in terms of compound weight is added in the Preliminary fermentation liquid that step 2 is obtained
Agent, stirs, and then seals fermentation tank, is fermented；Fermentation temperature is 40-50 DEG C；Fermentation total time is 10-15 days,
And since the 3rd day, stirring zymotic fluid 1 time, each 0.5-1 hours daily；The mixed fermenting agent is Cellvibrio, lichens
The mix bacterium agent of bacillus, streptomycete and candida tropicalis, its weight ratio is 1-2: 1-2: 1-2: 1-2；
Step 4：After the completion of fermentation, zymotic fluid is stood and abandons precipitation, and remaining ferment liquid concentrated at 45-65 DEG C, is done
It is dry, solid content is obtained, further ground crushing obtains sugar refinery discarded object fermentation powder.
Preferably, sugar refinery discarded object fermentation powder of the present invention is obtained by the following method：
Step 1：Sugar refinery discarded object compound is prepared, the compound is mixed to get by bagasse, filter mud and molasses, and its is heavy
Amount is than being 4-6: 3-5: 2-4；
Step 2：The compound that step 1 is obtained is added in fermentation tank, is subsequently adding the about 70%- in terms of compound weight
90% water, and stir, obtain Preliminary fermentation liquid；
Step 3：To the mixed fermentation bacterium that the about 4-8% in terms of compound weight is added in the Preliminary fermentation liquid that step 2 is obtained
Agent, stirs, and then seals fermentation tank, is fermented；Fermentation temperature is 42-45 DEG C；Fermentation total time is 12-15 days,
And since the 3rd day, stirring zymotic fluid 1 time, each 0.5-1 hours daily；The mixed fermenting agent is Cellvibrio, lichens
The mix bacterium agent of bacillus, streptomycete and candida tropicalis, its weight ratio is 1-2: 1-2: 1-2: 1-2；
Step 4：After the completion of fermentation, zymotic fluid is stood and abandons precipitation, and remaining ferment liquid concentrated at 50-60 DEG C, is done
It is dry, solid content is obtained, further ground crushing obtains sugar refinery discarded object fermentation powder.
It is furthermore preferred that sugar refinery discarded object fermentation powder of the present invention is obtained by the following method：
Step 1：Sugar refinery discarded object compound is prepared, the compound is mixed to get by bagasse, filter mud and molasses, and its is heavy
Amount is than being 5: 3: 2；
Step 2：The compound that step 1 is obtained is added in fermentation tank, is subsequently adding in terms of compound weight about 80%
Water, and stir, obtain Preliminary fermentation liquid；
Step 3：To the mixed fermenting agent that in terms of compound weight about 6% is added in the Preliminary fermentation liquid that step 2 is obtained,
Stir, then seal fermentation tank, fermented；Fermentation temperature is 45 DEG C；Fermentation total time is 15 days, and from the 3rd day
Start, daily stirring zymotic fluid 1 time, every time 0.5 hour；The mixed fermenting agent is Cellvibrio, bacillus licheniformis, chain
Mould and the mix bacterium agent of candida tropicalis, its weight ratio are 2: 1: 1: 2；
Step 4：After the completion of fermentation, zymotic fluid is stood and abandons precipitation, and remaining ferment liquid concentrated at 55 DEG C, is dried,
Solid content is obtained, further ground crushing obtains sugar refinery discarded object fermentation powder.
Wherein, the number of viable of present invention fermenting agent used is more than 0.5 hundred million cfu/g, preferably greater than 100,000,000 cfu/g, for example
The cfu/g of 1-20 hundred million；The viable count of bacillus subtilis powder used of the invention is more than 15,000,000,000 cfu/g.
On the other hand, the invention further relates to the biological organic fertilizer containing bacillus subtilis of described utilization sugar refinery discarded object
Preparation method, specially：
(1) sugar refinery discarded object fermentation powder is prepared by following methods
Step 1：Sugar refinery discarded object compound is prepared, the compound is mixed to get by bagasse, filter mud and molasses, and its is heavy
Amount is than being 2-8: 2-8: 1-4；
Step 2：The compound that step 1 is obtained is added in fermentation tank, is subsequently adding the about 50%- in terms of compound weight
150% water, and stir, obtain Preliminary fermentation liquid；
Step 3：To the mixed fermentation bacterium that the about 1-10% in terms of compound weight is added in the Preliminary fermentation liquid that step 2 is obtained
Agent, stirs, and then seals fermentation tank, is fermented；Fermentation temperature is 40-50 DEG C；Fermentation total time is 10-15 days,
And since the 3rd day, stirring zymotic fluid 1 time, each 0.5-1 hours daily；The mixed fermenting agent is Cellvibrio, lichens
The mix bacterium agent of bacillus, streptomycete and candida tropicalis, its weight ratio is 1-2: 1-2: 1-2: 1-2；
Step 4：After the completion of fermentation, zymotic fluid is stood and abandons precipitation, and remaining ferment liquid concentrated at 45-65 DEG C, is done
It is dry, solid content is obtained, further ground crushing obtains sugar refinery discarded object fermentation powder；
(2) sugar refinery discarded object fermentation powder, bacillus subtilis powder, the auxiliary agent of formula ratio are weighed, uniform, bag is mixed
Dress, you can obtain biological organic fertilizer of the invention.
In another preparation method of the invention, described sugar refinery discarded object fermentation powder is preferably made by the following method
It is standby：
Step 1：Sugar refinery discarded object compound is prepared, the compound is mixed to get by bagasse, filter mud and molasses, and its is heavy
Amount is than being 4-6: 3-5: 2-4；
Step 2：The compound that step 1 is obtained is added in fermentation tank, is subsequently adding the about 70%- in terms of compound weight
90% water, and stir, obtain Preliminary fermentation liquid；
Step 3：To the mixed fermentation bacterium that the about 4-8% in terms of compound weight is added in the Preliminary fermentation liquid that step 2 is obtained
Agent, stirs, and then seals fermentation tank, is fermented；Fermentation temperature is 42-45 DEG C；Fermentation total time is 12-15 days,
And since the 3rd day, stirring zymotic fluid 1 time, each 0.5-1 hours daily；The mixed fermenting agent is Cellvibrio, lichens
The mix bacterium agent of bacillus, streptomycete and candida tropicalis, its weight ratio is 1-2: 1-2: 1-2: 1-2；
Step 4：After the completion of fermentation, zymotic fluid is stood and abandons precipitation, and remaining ferment liquid concentrated at 50-60 DEG C, is done
It is dry, solid content is obtained, further ground crushing obtains sugar refinery discarded object fermentation powder.
In a kind of preparation method of the invention, described sugar refinery discarded object fermentation powder is more preferably made by the following method
It is standby：
Step 1：Sugar refinery discarded object compound is prepared, the compound is mixed to get by bagasse, filter mud and molasses, and its is heavy
Amount is than being 5: 3: 2；
Step 2：The compound that step 1 is obtained is added in fermentation tank, is subsequently adding in terms of compound weight about 80%
Water, and stir, obtain Preliminary fermentation liquid；
Step 3：To the mixed fermenting agent that in terms of compound weight about 6% is added in the Preliminary fermentation liquid that step 2 is obtained,
Stir, then seal fermentation tank, fermented；Fermentation temperature is 45 DEG C；Fermentation total time is 15 days, and from the 3rd day
Start, daily stirring zymotic fluid 1 time, every time 0.5 hour；The mixed fermenting agent is Cellvibrio, bacillus licheniformis, chain
Mould and the mix bacterium agent of candida tropicalis, its weight ratio are 2: 1: 1: 2；
Step 4：After the completion of fermentation, zymotic fluid is stood and abandons precipitation, and remaining ferment liquid concentrated at 55 DEG C, is dried,
Solid content is obtained, further ground crushing obtains sugar refinery discarded object fermentation powder.
In preparation method of the invention, the auxiliary agent preferred embodiment is as described in before-mentioned products technical scheme；
In preparation method of the invention, the fermenting agent preferred embodiment is as described in before-mentioned products technical scheme；
In preparation method of the invention, each component content preferred embodiment such as before-mentioned products technical scheme institute
State.
On the other hand, the invention further relates to the use of the biological organic fertilizer containing bacillus subtilis of described utilization sugar refinery discarded object
It is specially dilute with water in the Growing season of plant using the biological organic fertilizer as foliar fertilizer in the purposes for improving plant growth proterties
100-500 times, preferably 200-400 times administration of spraying is released, dilution spray amount is 30-120kg/ mus, preferably 50-80kg/ mu.
In purposes of the invention, the preferred sugarcane of plant；
In purposes of the invention, the plant trait for preferably improving is selected from following one or more：Increase blade and blade green
Cellulose content, increase plant height, increase stem diameter.
In embodiments of the invention, the bagasse, filter mud and molasses for example can be obtained from cane sugar factory, wherein
The water content of bagasse and filter mud is less than 30%；The fermenting agent and auxiliary agent can be commercially available.
Beneficial effects of the present invention are：
1st, the present invention uses bagasse, filter mud and the molasses mixture of special ratios as fermentation raw material, both effectively utilizes
Sugar refinery discarded object, while effectively prevent when only applying molasses as fermentation raw material, it is understood that there may be remaining sucrose, influences plant
The hidden danger grown, and solve when using only bagasse and/or filter mud as fermentation raw material, fermenting agent propagation institute
The problem of the nutrient deficiency for needing；
2nd, the present invention uses liquid fermentation method, the method relative to solid fermentation method, can be ferment it is more thorough；
3rd, the present invention it is final prepare solid foliar fertilizer, relative to liquid foliage fertilizer, packing and the link such as transporting
Operation is more prone to；
4th, the fermentation solid powder and bacillus subtilis that specifically fermentation method of the present invention is obtained are used cooperatively, relative to each
It is used alone, the growth traits of plant can be significantly improved.
Specific embodiment
In order to understand the present invention, the present invention is further illustrated with embodiment below, but do not limit the present invention.Wherein, under
In stating embodiment, the water content of bagasse used and filter mud between 10-15%, the number of viable about 5-6 hundred million of fermenting agent used
Cfu/g, the cfu/g of viable count about 20,000,000,000 of bacillus subtilis powder used
(1) product preparation example
Embodiment 1
The formula of biological organic fertilizer is：
Its preparation method is：Bagasse 50kg, filter mud 30kg, molasses 20kg are weighed, and is mixed；Then, it is added into
In fermentation tank, and 80kg water is added, and stirred；Be subsequently adding 6kg by Cellvibrio, bacillus licheniformis, streptomycete with
And the mixed fermenting agent (weight ratio is 2: 1: 1: 2) of candida tropicalis composition, stir, then fermentation tank is sealed,
Fermented；Fermentation temperature is 45 DEG C；Fermentation total time is 15 days, and since the 3rd day, stirs zymotic fluid 1 time daily, every time
0.5 hour.After the completion of fermentation, zymotic fluid is stood and abandons precipitation, and remaining ferment liquid concentrated at 55 DEG C, is dried, consolidate
Shape thing, further ground crushing obtains sugar refinery discarded object fermentation powder.
Weigh 7kg sugar refinery discarded object fermentation powder obtained above, 2kg bacillus subtilis powders, 0.5kg N- lauroyl
Base sodium glutamate, 0.5kg sodium lignin sulfonates, are mixed uniform, packaging, you can obtain biological organic fertilizer of the invention.
Embodiment 2
The formula of biological organic fertilizer is：
Its preparation method is：Bagasse 40kg, filter mud 40kg, molasses 20kg are weighed, and is mixed；Then, it is added into
In fermentation tank, and 70kg water is added, and stirred；Be subsequently adding 4kg by Cellvibrio, bacillus licheniformis, streptomycete with
And the mixed fermenting agent (weight ratio is 2: 1: 1: 2) of candida tropicalis composition, stir, then fermentation tank is sealed,
Fermented；Fermentation temperature is 48 DEG C；Fermentation total time is 12 days, and since the 3rd day, stirs zymotic fluid 1 time daily, every time
0.5 hour.After the completion of fermentation, zymotic fluid is stood and abandons precipitation, and remaining ferment liquid concentrated at 55 DEG C, is dried, consolidate
Shape thing, further ground crushing obtains sugar refinery discarded object fermentation powder.
Weigh 8kg sugar refinery discarded object fermentation powder obtained above, 1.2kg bacillus subtilis powders, 0.4kg sulfomethylated lignins
Sour calcium, 0.4kg penetrating agent JFCs, are mixed uniform, packaging, you can obtain biological organic fertilizer of the invention.
Embodiment 3
The formula of biological organic fertilizer is：
Its preparation method is：Bagasse 70kg, filter mud 50kg, molasses 30kg are weighed, and is mixed；Then, it is added into
In fermentation tank, and 150kg water is added, and stirred；12kg is subsequently adding by Cellvibrio, bacillus licheniformis, streptomycete
And the mixed fermenting agent (weight ratio is 2: 1: 1: 2) of candida tropicalis composition, stir, it is then that fermentation tank is close
Envelope, is fermented；Fermentation temperature is 40 DEG C；Fermentation total time is 15 days, and since the 3rd day, stirs zymotic fluid 1 time daily,
0.5 hour every time.After the completion of fermentation, zymotic fluid is stood and abandons precipitation, and remaining ferment liquid concentrated at 55 DEG C, is dried, obtained
To solid content, further ground crushing obtains sugar refinery discarded object fermentation powder.
Weigh 6.5kg sugar refinery discarded object fermentation powder obtained above, 2.5kg bacillus subtilis powders, 0.6kgN- bays
Acyl glutamic acid sodium, 0.4kg soap powder, are mixed uniform, packaging, you can obtain biological organic fertilizer of the invention.
Comparative example 1
Load 200L disinfection fermentation tanks after blackstrap 100kg, water 100kg are sterilized, when its temperature is dropped to below 35 DEG C
Inoculation aerophile bacterium, saccharomycete and the common 2kg of actinomadura roseorufa micro organisms strain, and stirred evenly with sterilizing waddy.Meanwhile, in fermentation tank mouth
3 layers of gauze of covering, carries out cultivation and fermentation.Start within the 4th day in fermentation, be aerated (morning and afternoon each 30min) to it daily,
Until producing within the 26th day dense aroma to stop intermittent aerating, liquid biological foliar fertilizer is obtained.
Comparative example 2
Bagasse 50kg, filter mud 30kg, molasses 20kg are weighed, and is mixed；Then, it is added into fermentation tank, and adds
Enter 80kg water, and stir；6kg is subsequently adding by Cellvibrio, bacillus licheniformis, streptomycete and candida tropicalis
The mixed fermenting agent (weight ratio is 2: 1: 1: 2) of composition, stirs, and then seals fermentation tank, is fermented；Fermentation temperature
Spend is 45 DEG C；Fermentation total time is 15 days, and since the 3rd day, stirs zymotic fluid 1 time, every time 0.5 hour daily.Ferment
Cheng Hou, zymotic fluid is stood and abandons precipitation, and remaining ferment liquid is concentrated at 55 DEG C, is dried, and solid content is obtained, further through grinding
Milling is broken to obtain sugar refinery discarded object fermentation powder.
(2) test example
Sugar cane breed：Osmanthus sugar 29；
Experimental design：Sandy loam plot is chosen, 20 cells, plot area about 40m are divided into2, grow cane, go forward side by side
Row conventional fertilizer application, management of watering.Experiment sets 6 treatment altogether, often processes 3 repetitions, and a blank, 2 repetitions are tested small
Area's random alignment.For each treatment, respectively at tillering stage, Early period of cane elongation, the great elongation stage foliage-spray tie substance as described below,
Whole Growing season is sprayed 3 times altogether, and dilution spray amount is 60kg/ mus；For blank, in each period foliage-spray and treatment
The clear water of 1 equivalent.
Treatment 1：The product of the embodiment of the present invention 1,200 times of extension rate；
Treatment 2：The product of the embodiment of the present invention 2,200 times of extension rate；
Treatment 3：The product of the embodiment of the present invention 3,200 times of extension rate；
Treatment 4：The product of comparative example 1,30 times of extension rate；
Treatment 5：The product of comparative example 2,200 times of extension rate；
Treatment 6：1000000000 gemma/g bacillus subtilis wettable powders, 600 times of extension rate；
Measuring chlorophyll content：After last time foliage-spray 15 days, each treatment healthy leaves is randomly selected respectively to be carried out
Chlorophyll content is analyzed.The measure of chlorophyll content is edited according to Chen Jianxun, publishing house of South China Science ＆ Engineering University publishes《Plant gives birth to
Experiment instruction (second edition) of science》Described in method carry out, the content of chlorophyll a, b is determined in 663nm and 645nm respectively,
And then calculate the total content of blade Determination of Chlorophyll.
Plant height is determined：In the technical maturity of sugarcane, randomly select the healthy sugarcane strain of each treatment by 5 point samplings and surveyed
It is fixed, average；
Stem diameter is determined：In the technical maturity of sugarcane, randomly select the healthy sugarcane strain of each treatment by 5 point samplings and surveyed
It is fixed, average；
Each treatment measurement result is as in the table below.
| Treatment | Chlorophyll content (mg/g FW) | Plant height (mm) | Stem diameter (mm) |
| Treatment 1 | 6.10 | 315 | 32.4 |
| Treatment 2 | 5.82 | 305 | 31.7 |
| Treatment 3 | 5.78 | 301 | 30.9 |
| Treatment 4 | 4.56 | 263 | 26.9 |
| Treatment 5 | 5.10 | 276 | 27.8 |
| Treatment 6 | 4.24 | 252 | 25.9 |
| Blank | 4.25 | 248 | 25.5 |
It is not difficult to find out by above-mentioned data：
In terms of chlorophyll content in leaf blades, after being processed using product of the present invention (embodiment 1-3 products process 1-3), relatively
In blank and the control liquid foliar fertilizer of molasses fermented preparation, (4) product of comparative example 1, treatment, can substantially increase sweet
The chlorophyll total amount of sugarcane blade.Further, it is not difficult to find out by above-mentioned data, using sugar refinery discarded object yeast powder, (comparative example 2 is produced
Product, after 5) treatment is processed, can to a certain extent increase the chlorophyll total amount of Sugarcane Leaves, with bacillus subtilis (treatment
6) after processing, the chlorophyll total amount of Sugarcane Leaves is suitable with blank, and both are combined and combined in appropriate proportions by the present invention
After use, relative to the treatment for only using sugar refinery discarded object yeast powder, the chlorophyll that further can substantially increase Sugarcane Leaves is total
Amount, namely the present invention will it is above-mentioned both combine after, generate unexpected effect.
In terms of sugarcane plant height, after being processed using product of the present invention (embodiment 1-3 products process 1-3), relative to blank
(4) product of comparative example 1, treatment, can substantially increase the strain of sugarcane to the control liquid foliar fertilizer of control and molasses fermented preparation
It is high.Further, be not difficult to find out by above-mentioned data, using sugar refinery discarded object yeast powder (product of comparative example 2, after 5) treatment is processed,
Sugarcane plant height can to a certain extent be increased, after bacillus subtilis (treatment 6) treatment, sugarcane plant height and blank phase
When, and the present invention combines both after being applied in combination in appropriate proportions, relative to the treatment for only using sugar refinery discarded object yeast powder, energy
It is enough further substantially to increase sugarcane plant height, namely the present invention will it is above-mentioned both combine after, generate unexpected effect.
In terms of sugarcane stem diameter, after being processed using product of the present invention (embodiment 1-3 products process 1-3), relative to blank
(4) product of comparative example 1, treatment, can substantially increase the stem of sugarcane to the control liquid foliar fertilizer of control and molasses fermented preparation
Footpath.Further, be not difficult to find out by above-mentioned data, using sugar refinery discarded object yeast powder (product of comparative example 2, after 5) treatment is processed,
Sugarcane stem diameter can to a certain extent be increased, after bacillus subtilis (treatment 6) treatment, sugarcane stem diameter and blank phase
When, and the present invention combines both after being applied in combination in appropriate proportions, relative to the treatment for only using sugar refinery discarded object yeast powder, energy
It is enough further substantially to increase sugarcane stem diameter, namely the present invention will it is above-mentioned both combine after, generate unexpected effect.
The present invention is described by above-mentioned specific embodiment.But examples detailed above be only to it is of the invention enter
One step is explained, is not limitation of the invention, by carrying out such scheme simply to adjust the scheme obtained from, is not had
Depart from spirit of the invention, be regarded as being included within the scope of the present invention.
Claims (7)
1. a kind of biological organic fertilizer containing bacillus subtilis of utilization sugar refinery discarded object, the biological organic fertilizer includes such as the following group
Divide and its weight content：
Sugar refinery discarded object fermentation powder 40-90%
Bacillus subtilis powder 5-40%
Auxiliary agent 5-20%；
Sugar refinery discarded object fermentation powder is obtained by the following method：
Step 1：Sugar refinery discarded object compound is prepared, the compound is mixed to get by bagasse, filter mud and molasses, its weight ratio
It is 2-8: 2-8: 1-4；
Step 2：The compound that step 1 is obtained is added in fermentation tank, is subsequently adding the 50%-150% in terms of compound weight
Water, and stir, obtain Preliminary fermentation liquid；
Step 3：To the mixed fermenting agent that the 1-10% in terms of compound weight is added in the Preliminary fermentation liquid that step 2 is obtained, stir
Mix uniform, then seal fermentation tank, fermented；Fermentation temperature is 40-50 DEG C；Fermentation total time is 10-15 days, and from the
3 days start, and stirring zymotic fluid 1 time, each 0.5-1 hours daily；The mixed fermenting agent is Cellvibrio, lichens gemma bar
The mix bacterium agent of bacterium, streptomycete and candida tropicalis, its weight ratio is 1-2: 1-2: 1-2: 1-2；
Step 4：After the completion of fermentation, zymotic fluid is stood and abandons precipitation, and remaining ferment liquid concentrated at 45-65 DEG C, is dried, obtained
To solid content, further ground crushing obtains sugar refinery discarded object fermentation powder.
2. a kind of biological organic fertilizer containing bacillus subtilis of utilization sugar refinery discarded object, it is characterised in that the biological organic
Fertilizer is solid-state foliar fertilizer, and the biological organic fertilizer includes following component and its weight content：
Sugar refinery discarded object fermentation powder 60-80%
Bacillus subtilis powder 10-30%
Auxiliary agent 6-15%；
Sugar refinery discarded object fermentation powder is obtained by the following method：
Step 1：Sugar refinery discarded object compound is prepared, the compound is mixed to get by bagasse, filter mud and molasses, its weight ratio
It is 2-8: 2-8: 1-4；
Step 2：The compound that step 1 is obtained is added in fermentation tank, is subsequently adding the 50%-150% in terms of compound weight
Water, and stir, obtain Preliminary fermentation liquid；
Step 3：To the mixed fermenting agent that the 1-10% in terms of compound weight is added in the Preliminary fermentation liquid that step 2 is obtained, stir
Mix uniform, then seal fermentation tank, fermented；Fermentation temperature is 40-50 DEG C；Fermentation total time is 10-15 days, and from the
3 days start, and stirring zymotic fluid 1 time, each 0.5-1 hours daily；The mixed fermenting agent is Cellvibrio, lichens gemma bar
The mix bacterium agent of bacterium, streptomycete and candida tropicalis, its weight ratio is 1-2: 1-2: 1-2: 1-2；
Step 4：After the completion of fermentation, zymotic fluid is stood and abandons precipitation, and remaining ferment liquid concentrated at 45-65 DEG C, is dried, obtained
To solid content, further ground crushing obtains sugar refinery discarded object fermentation powder.
3. biological organic fertilizer according to claim 1 and 2, the auxiliary agent includes wetting dispersing agent, bleeding agent, wherein,
Described wetting dispersing agent is selected from the one kind in calcium lignosulfonate, N- sodium lauroyl glutamates, sldium lauryl sulfate
Or it is various, its weight content is 2.5-10%；
Described bleeding agent is selected from one or more in soap powder, sodium lignin sulfonate, penetrating agent JFC, and its weight content is
2.5-10%.
4. it is a kind of according to claim any one of 1-3 using sugar refinery discarded object the biological organic fertilizer containing bacillus subtilis
Preparation method, it is characterised in that
(1) sugar refinery discarded object fermentation powder is prepared by following methods
Step 1：Sugar refinery discarded object compound is prepared, the compound is mixed to get by bagasse, filter mud and molasses, its weight ratio
It is 2-8: 2-8: 1-4；
Step 2：The compound that step 1 is obtained is added in fermentation tank, is subsequently adding the 50%-150% in terms of compound weight
Water, and stir, obtain Preliminary fermentation liquid；
Step 3：To the mixed fermenting agent that the 1-10% in terms of compound weight is added in the Preliminary fermentation liquid that step 2 is obtained, stir
Mix uniform, then seal fermentation tank, fermented；Fermentation temperature is 40-50 DEG C；Fermentation total time is 10-15 days, and from the
3 days start, and stirring zymotic fluid 1 time, each 0.5-1 hours daily；The mixed fermenting agent is Cellvibrio, lichens gemma bar
The mix bacterium agent of bacterium, streptomycete and candida tropicalis, its weight ratio is 1-2: 1-2: 1-2: 1-2；
Step 4：After the completion of fermentation, zymotic fluid is stood and abandons precipitation, and remaining ferment liquid concentrated at 45-65 DEG C, is dried, obtained
To solid content, further ground crushing obtains sugar refinery discarded object fermentation powder；
(2) sugar refinery discarded object fermentation powder, bacillus subtilis powder, the auxiliary agent of formula ratio are weighed, uniform, packaging is mixed,
Can obtain biological organic fertilizer of the invention.
5. a kind of biological organic fertilizer described in any one of claim 1-3 is prepared by claim 4 methods described
Biological organic fertilizer is used to improve the purposes of plant growth proterties.
6. purposes according to claim 5, it is characterised in that the plant is sugarcane.
7. purposes according to claim 5, it is characterised in that the plant trait of the improvement is selected from following a kind of or many
Kind：Increase chlorophyll content in leaf blades, increase plant height, increase stem diameter.
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| CN201510057269.8A CN104609977B (en) | 2015-02-04 | 2015-02-04 | A kind of biological organic fertilizer containing bacillus subtilis of utilization sugar refinery discarded object |
| AU2015100945A AU2015100945A4 (en) | 2015-02-04 | 2015-07-16 | Bio-organic fertilizer containing Bacillus subtilis |
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| CN109438006B (en) * | 2018-11-19 | 2021-12-28 | 南京工业大学 | Preparation method of aeroponic green agricultural biological product nutrient solution |
| CN109438069A (en) * | 2018-12-13 | 2019-03-08 | 中山市承铭农业技术开发有限公司 | A method of utilizing sugar refinery leftover bits and pieces Amino acid liquid fertilizer |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1171384A (en) * | 1997-05-20 | 1998-01-28 | 陈立平 | Process for producing active organic compound fertilizer with waste liquid of molasses alcohol |
| CN1412157A (en) * | 2002-12-06 | 2003-04-23 | 广东益万家食品有限公司 | Preparation of active biological organic fertilizer by utilizing bagasse and lime sludge |
| CN101250066A (en) * | 2008-04-03 | 2008-08-27 | 桂林工学院 | Method for producing biological leaf fertilizer by using waste molasses of sugar plant |
| CN103396232A (en) * | 2013-08-01 | 2013-11-20 | 京博农化科技股份有限公司 | Biological fertilizer |
| CN103896659A (en) * | 2014-01-23 | 2014-07-02 | 叶长东 | Humic acid type fluid or solid organic fertilizer composition utilizing wastes from sugar mills, alcohol plants, gourmet powder factories and yeast plants |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007015881A (en) * | 2005-07-06 | 2007-01-25 | Katsuo Uchida | Organic fertilizer |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1171384A (en) * | 1997-05-20 | 1998-01-28 | 陈立平 | Process for producing active organic compound fertilizer with waste liquid of molasses alcohol |
| CN1412157A (en) * | 2002-12-06 | 2003-04-23 | 广东益万家食品有限公司 | Preparation of active biological organic fertilizer by utilizing bagasse and lime sludge |
| CN101250066A (en) * | 2008-04-03 | 2008-08-27 | 桂林工学院 | Method for producing biological leaf fertilizer by using waste molasses of sugar plant |
| CN103396232A (en) * | 2013-08-01 | 2013-11-20 | 京博农化科技股份有限公司 | Biological fertilizer |
| CN103896659A (en) * | 2014-01-23 | 2014-07-02 | 叶长东 | Humic acid type fluid or solid organic fertilizer composition utilizing wastes from sugar mills, alcohol plants, gourmet powder factories and yeast plants |
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| Title |
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| 广辟蔗区肥源以蔗养蔗;叶成秋;《广东农业科学》;19880430(第2期);第18-20页 * |
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