CN104605160B - A kind of compositions preventing and treating sow puerperal endometritis and application thereof - Google Patents
A kind of compositions preventing and treating sow puerperal endometritis and application thereof Download PDFInfo
- Publication number
- CN104605160B CN104605160B CN201510027670.7A CN201510027670A CN104605160B CN 104605160 B CN104605160 B CN 104605160B CN 201510027670 A CN201510027670 A CN 201510027670A CN 104605160 B CN104605160 B CN 104605160B
- Authority
- CN
- China
- Prior art keywords
- sow
- resveratrol
- endometritis
- vitamin
- compositions
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention discloses a kind of compositions preventing and treating sow puerperal endometritis and application thereof；Said composition includes following components: resveratrol, vitamin C and the carrier of feed additive license and essence；Described compositions is applied to that sow is antenatal and 7 days puerperal, it is possible to significantly inhibit synthesis and the secretion of the neutrophilic chemotactic factor of endometrium local pathogenic bacterium inducing, lower the effect of the incidence rate of endometritis as feed additive.It is convenient that said composition is added, instant effect, it is entirely avoided toxic and side effects that antibiotic is caused and drug resistance.
Description
Technical field
The present invention relates to a kind of compositions preventing and treating sow puerperal endometritis and application thereof, belong to pannage additive technology
Field.
Background technology
Gilt Uterus intimitis is common disease during Pig Industry produces, sickness rate typically 15%～about 20%, minority pig farm
The sickness rate of Gilt Uterus intimitis is up to more than 30%.Mostly the generation of this disease is to sterilize in Farrowing or Process of Insemination not tight,
Bring into caused by pathogen.It is impaired that Gilt Uterus intimitis can directly result in uterine function, loses fertility, is to cause producing
One of Important cause of disease that sow is eliminated.This disease also can cause sow not eat puerperal, and the milk water yield declines, and causes nascent piglets
Mortality rate rises.
Modern medicine study thinks that the pathogenic factor of Gilt Uterus intimitis is that pathogenic microorganism invades Gilt Uterus, causes uterus to feel
Dye, and form inflammatory reaction.The pathogenic microorganism causing endometritis mainly has: escherichia coli, streptococcus, staphylococcus,
Pseudomonas aeruginosa, corynebacterium pyogenes, klebsiella, tubercule bacillus, Salmonella, candidiasis, actinomycetes, Mucor
The multiple-microorganisms such as bacterium.Relate to the kinds such as virus, antibacterial, fungus, mycoplasma.Gilt Uterus is then thought in traditional medicine research
Intimitis belongs to damp and hot knot, the stagnancy of qi and blood stasis of becoming silted up, and its dominant mechanism occurred is that vessels of the uterus is hollow, and the turbid liquid of remaining blood is not clean, as
Puerperium is unclean, infects damp and hot pathogen；Or sterilization is not strict, the heresy causing damp and hot poison is taken advantage of a weak point, and in retardance born of the same parents, vessels of the uterus QI and blood is transported
Capable not smooth, punching is appointed impaired and is fallen ill.
The existing prophylactico-therapeutic measures for endometritis mainly use antimicrobial susceptibility antibiotic (as oxytetracycline, ofloxacin,
Metronidazole, amoxicillin etc.) treat, or use some to have heat-clearing and toxic substances removing, blood stasis dispersing and fresh blood promoting, nourshing blood and promoting blood circulation, dispel
Lochia, the Chinese herbal medicine and the preparation (such as Herba Leonuri, Flos Carthami, Radix Angelicae Sinensis, Rhizoma Chuanxiong, SHENGHUATANG etc.) thereof that shrink uterus are treated.
These methods, while obtained using widely in production practices, but it is poor to there is obvious prevention effect, and the many grades of side effect lack
Fall into.It main reason is that antibiotics easily produces drug resistance, and toxic and side effects is big, in injection or uterine perfusion mistake
In journey, operation element amount is big, the defects such as sow stress is big.Simple Chinese herbal medicine extract is used then to there is slow curative effect, effect
The shortcomings such as fruit is the most certain.
Summary of the invention
The present invention is directed to prior art is prevented and treated the method for sow puerperal endometritis and have that toxic and side effects is big, be easily formed fastbacteria
The defect of the aspects such as strain and curative effect instability, it is therefore intended that a kind of convenient drug administration is provided, can effectively prevent and treat Gilt Uterus intimitis
Compositions and application thereof.
A kind of compositions preventing and treating Gilt Uterus intimitis, said composition is made up of resveratrol and vitamin C, both weight ratios
For 1:10-10:1.It is preferably 1:5-5:1.More preferably 1:3-3:1.Much further preferably from 1:3-1:1.Most preferably
For 1:2.
The compositions of described preventing and treating Gilt Uterus intimitis, also can add carrier and the essence of feed additive license.Preferably carry
Body can be Semen Maydis powder, rice chaff powder, glucose, precipitated calcium carbonate etc., and the mass ratio of carrier controls at 10%-95%, essence
Can be preferably U.S. giving off a strong fragrance, big Supreme Being is fragrant.The mass ratio of essence controls at 0.1%-0.5%.
The application process of the compositions of described preventing and treating Gilt Uterus intimitis, adds to described compositions and is used in feedstuff preventing and treating
Gilt Uterus intimitis.Specifically by compositions sow is antenatal and puerperal adds feeding sow in sow feed to.More specifically
Compositions was added in sow feed feeding sow during first 7 days to 7 days puerperal of sow expected date of confinement；Feeding 2-3 at least continuously
My god.
Described compositions addition in feedstuff is 0.05～1.0wt%.
The present invention is mainly based upon inventor and for pathogenetic further investigation of endometritis and re-recognizes.Real producing
In trampling, it is a universal phenomenon that sow pathogen in puerperal enters Gilt Uterus, but final major part sow all can rely on uterus certainly
The immunologic mechanism of body disposes invasion pathogen in time, it is ensured that uterus is not by pathogenic bacterial infection, but minority sow is due to uterus or machine
The function of immune system of body is abnormal, it is impossible to quickly disposes pathogen, thus causes inflammation to occur.Endometritis is ground
In studying carefully, find that mostly the sow suffering from endometritis is owing to Uterine mucosa barrier breakdown, uterine bacteria and toxin persistently invade, swash
Local, uterus alive leucocytes system, especially mononuclear phagocyte system, leukocyte is in the excessive gathering in local inflammation region, shape
Become damage type damage, and and then discharge a large amount of cytokines and inflammatory mediator, the neutrophilic granulocyte of endometrium local in localized sustained
Chemotactic factor (CINC-1, CINC-2, CINC-3) and lipopolysaccharide-induced Gro-beta-T (LIX) express notable rising,
Produce the active oxygen of excess, and further induction inflammatory cell release proinflammatory factor.Thus it is anti-to start waterfall type cascade inflammation
Should, cause excessive inflammatory reaction.
Based on this understanding, it is known that the invasion uterus of pathogenic microorganism not necessarily causes the generation of endometritis.Body without
Method effectively suppresses the LCF of pathogen-inducible, causes out of control being only of inflammatory reaction to cause the main of endometritis
Reason.Therefore, the waterfall type cascade inflammatory reaction that the most in time, quickly suppression inflammatory factor causes at pathogenic microorganism is only
The key of preventing and treating endometritis.
On the basis of this understanding, screen the combination that can suppress inflammatory cytokine in early days, find resveratrol and Wei Sheng
The use in conjunction of element C has obvious inhibition to inflammatory cytokine.Resveratrol (3,5,4 '-trihydroxy-1,2-hexichol
Ethylene) it is a kind of natural polyphenol class antioxidant, it is possible to and effective suppression leukocyte oozes out in blood capillary, suppresses Semen arachidis hypogaeae four
The release of olefin(e) acid, scavenging activated oxygen, it is possible to the most effectively stop the generation of cascade of response of inflammation in inflammation, prevent excessive inflammation
Disease is reacted.
Vitamin C is also L ascorbic acid, it is possible to reduce capillary permeability and fragility, reduces tissue infiltration, plays detumescence
With the effect promoting wound healing, it is possible to play synergism with resveratrol.
The present invention Inhibition test by LCF, finds resveratrol and ascorbic use in conjunction, it is possible to ratio
Use one of which more can effectively suppress the effect of lipopolysaccharide-induced LCF.Its safety experiment also indicates that,
This combination is without any side effects for sow, and can effectively improve piglet survival ratio.Effect oestrus again also after sow wean
Become apparent from.
Said composition is added in pig feed as additive, can effectively prevent and treat the generation of the puerperal endometritis of sow, this
Bright product has environmental protection, curative effect really and is not likely to produce drug resistance and is difficult to the features such as recurrence.And this invention is in feedstuff
It is convenient to add, and will not produce the stress causing sow injection or intrauterine administration.
Detailed description of the invention
Below in conjunction with embodiment, the present invention, the scheme of embodiment described here are described, are not intended to the present invention, the professional people of this area
The improvement that it can be correlated with by member according to the present invention, these described improvement are regarded as within the scope of the invention.
Embodiment 1
Resveratrol and the vitamin C use in conjunction inhibitory action to lipopolysaccharide-induced Gro-beta-T:
First weigh by following mass ratio, then dissolve preparation resveratrol and ascorbic solution, set up blank group:
Sample 1 resveratrol: vitamin C is 1:0
Sample 2 resveratrol: vitamin C is 0:1
Sample 3 resveratrol: vitamin C is 1:2
Sample 4 resveratrol: vitamin C is 3:1
Sample 5 resveratrol: vitamin C is 2:3
Sample 6 resveratrol: vitamin C is 1:3
Sample 7 resveratrol: vitamin C is 2:1
Sample 8 resveratrol: vitamin C is 1:1
Sample 9 resveratrol: vitamin C is 3:2
Sample 10 blank (ultra-pure water group)
The total content of the solution's solute of above-mentioned preparation is 0.01wt%.Carry out the lipopolysaccharide-induced of rat endometrium cell respectively
The detection of the inhibitory action of Gro-beta-T.Its concrete grammar is: use the SD rat 1 in 1 day puerperal, scrapes intrauterine
Theca cell, after the trypsinization of 0.25%, by cell suspension inoculation in cell bottle, is placed in 37 DEG C, the carbon dioxide training of 5%
Support in case and cultivate.Cell after passing on is inoculated in 96 well culture plates, after cell again covers with and converges, for white Herba chenopodii
Reed alcohol and the vitamin C Inhibition test to lipopolysaccharide-induced Gro-beta-T.
LPS is added so that it is final concentration reaches 100 μ g/mL, sets up rat endometrium thin in the cell culture fluid of 96 orifice plates
The lipopolysaccharide-induced model of born of the same parents.Being respectively added in Tissue Culture Plate by the sample of above-mentioned preparation, each sample is added separately to 5
In individual hole.The Gro-beta-T that the detection of euzymelinked immunosorbent assay (ELISA) (ELISA) method is lipopolysaccharide-induced is used after continuing to cultivate 48 hours
(LIX) concentration.Acquired results is shown in Table 1.
| Sample number | LIX mean concentration (pg/mL) (n=5, Mean ± SD) |
| 1 | 545.82±143.44 |
| 2 | 665.24±133.54 |
| 3 | 311.26±21.75 |
| 4 | 413.84±147.58 |
| 5 | 398.74±64.25 |
| 6 | 348.56±65.88 |
| 7 | 496.67±84.32 |
| 8 | 356.82±74.87 |
| 9 | 409.53±38.72 |
| 10 | 2155.82±142.15 |
As shown in Table 1, resveratrol and vitamin C all lipopolysaccharide-induced Gro-beta-Ts (LIX) to endometrial cell
There is obvious inhibitory action, and at total soluble matters content in the case of 0.01%, resveratrol and ascorbic use in conjunction
Effect is better than the application of monomer.Its optimal proportioning is resveratrol and ascorbic mass ratio is about 1:2.
Embodiment 2
Resveratrol 0.25 kilogram, vitamin C 0.5 kilogram.And 9.24 kilograms of carrier being allowed of additive and essence 0.01
Kilogram, stir after often adding a component, finally obtain compositions.
Said composition is joined in sow complete feed in the ratio of 1% again, sow is antenatal and feeds in puerperal 0～7 days,
The feed intake of every sow every day controls at 3～8 kilograms.
Embodiment 3
Resveratrol 0.5 kilogram, vitamin C 1.0 kilograms.And 8.49 kilograms of carrier being allowed of additive and essence 0.01
Kilogram, stir after often adding a component, finally obtain compositions.
Said composition is joined in sow complete feed in the ratio of 1% again, sow is antenatal and feeds in puerperal 0～7 days,
The feed intake of every sow every day controls at 3～8 kilograms.
Embodiment 4
Resveratrol 0.25 kilogram, vitamin C 1.0 kilograms.And 8.49 kilograms of carrier being allowed of additive and essence 0.01
Kilogram, stir after often adding a component, finally obtain compositions.
Said composition is joined in sow complete feed in the ratio of 1% again, in sow puerperal 3～within 5 days, feed, every sow
The feed intake of every day controls at 3～8 kilograms.
Embodiment 5
Experiment effect:
Formula 1: resveratrol 0.25 kilogram, vitamin C 0.5 kilogram.And 9.24 kilograms of the carrier that additive is allowed
With 0.01 kilogram of essence, stir after often adding a component, finally obtain compositions.
Formula 2: 9.7 kilograms of the carrier that resveratrol 0.25 kilogram and additive are allowed and 0.01 kilogram of essence, often
Stir after adding a component, finally obtain compositions.
Formula 3: vitamin C 0.5 kilogram.And 9.54 kilograms of carrier being allowed of additive and 0.01 kilogram of essence, often
Stir after adding a component, finally obtain compositions.Sow 240 is chosen in experiment altogether, is randomly divided into experimental group and right
According to group totally two groups, often 60 sows of group, wherein every sow of experimental group sow is in antenatal and 3 days puerperal, i.e. perinatal stage totally 6 days
Adding this invention preparation continuously, matched group sow, without invention formulation, observes the sickness rate of sow puerperal endometritis.
Endometritis criterion is that sow is still discharged by lochia 4 days puerperal, and secretions cloudy state.Gilt Uterus intimitis
Sickness rate is shown in Table 2.
| Group | Morbidity sow number | Healthy sow number | Endometritis sickness rate |
| Formula 1 | 1 | 59 | 1.67%c |
| Formula 2 | 3 | 57 | 5%b |
| Formula 3 | 3 | 57 | 5%b |
| Matched group | 7 | 54 | 11.67%a |
Note: letter shoulder mark difference represents significant difference (P≤0.05)
As known from Table 2, formula 1, formula 2 and formula 3 all can significantly reduce the sickness rate of sow puerperal endometritis,
And the effect of formula 1 is better than formula 2 and formula 3.
Claims (1)
1. a resveratrol and vitamin C are in the purposes preparing the compositions preventing and treating Gilt Uterus intimitis, it is characterized in that, being used for preparing by described compositions and add the preparation being used for preventing and treating Gilt Uterus intimitis in feedstuff to, said composition is made up of by weight 1:2 resveratrol and vitamin C；Described compositions is also added with carrier and the essence of feed additive license, and carrier quality content is 10%～95%, and essence mass content is 0.1%～0.5%；Described compositions addition in feedstuff is 0.05-1.0wt%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510027670.7A CN104605160B (en) | 2015-01-20 | 2015-01-20 | A kind of compositions preventing and treating sow puerperal endometritis and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510027670.7A CN104605160B (en) | 2015-01-20 | 2015-01-20 | A kind of compositions preventing and treating sow puerperal endometritis and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104605160A CN104605160A (en) | 2015-05-13 |
| CN104605160B true CN104605160B (en) | 2016-08-17 |
Family
ID=53140036
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510027670.7A Active CN104605160B (en) | 2015-01-20 | 2015-01-20 | A kind of compositions preventing and treating sow puerperal endometritis and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104605160B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112704159A (en) * | 2020-11-18 | 2021-04-27 | 福建傲农生物科技集团股份有限公司 | Feed premix for reducing inflammation after delivery of sows and preparation and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104225096A (en) * | 2014-08-21 | 2014-12-24 | 杭州双马生物工程有限公司 | Immunity-enhancing capsule and preparation method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE502006008551D1 (en) * | 2005-02-04 | 2011-02-03 | Peter Heger | METHOD FOR PRODUCING A HYDROXYSTILLE-CONTAINING DRUG EXTRACT |
-
2015
- 2015-01-20 CN CN201510027670.7A patent/CN104605160B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104225096A (en) * | 2014-08-21 | 2014-12-24 | 杭州双马生物工程有限公司 | Immunity-enhancing capsule and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 中西医结合治疗母猪子宫内膜炎;崔芳年等;《中兽医学杂志》;20070228(第2期);18页第1、3.1以及5部分 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104605160A (en) | 2015-05-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Thompson et al. | Effect of cooling during the dry period on immune response after Streptococcus uberis intramammary infection challenge of dairy cows | |
| CN104688895B (en) | A kind of Traditional Chinese medicine probiotic compound formulation for preventing and treating chickens respiratory disease | |
| Zhong et al. | Polyphenol extracts from Punica granatum and Terminalia chebula are anti-inflammatory and increase the survival rate of chickens challenged with Escherichia coli | |
| WO2008097109A1 (en) | Tonic and food supplement comprising aloe vera and honey | |
| KR20100088389A (en) | Natural antibiotics comprising bee-venoms and crude drug-extracts and preparing method thereof | |
| CN107158087A (en) | It is a kind of to prevent Chinese medicine composition of hog cholera and preparation method thereof | |
| RU2624868C2 (en) | Preparation method of the complex gel for mastitis treatment and prevention in cows and complex gel on its basis | |
| CN104605160B (en) | A kind of compositions preventing and treating sow puerperal endometritis and application thereof | |
| CN104162162B (en) | A kind of anti-Schweineseuche compositions, lyophilized powder, preparation method and application | |
| CN102973690A (en) | Chinese herbal medicine preparation for treating pig gastroenteritis | |
| CN104162159B (en) | A kind of anti-dog parvovirus compositions, lyophilized powder, preparation method and application | |
| CN115624579A (en) | Traditional Chinese medicine composition for inhibiting aeromonas veronii and application thereof | |
| US20210128695A1 (en) | Ovotransferrin treatment for the reproductive tract | |
| CN103877521A (en) | Pharmaceutical composition for preventing and treating chicken colibacillosis and preparation method of pharmaceutical composition | |
| da Cruz et al. | Health benefits of honey | |
| CN104758347B (en) | Traditional Chinese medicine oral liquid for treating pullorum disease and preparation method thereof | |
| CN112043730A (en) | Traditional Chinese medicine biological preparation for preventing and treating animal epidemic diseases | |
| RU2524664C1 (en) | Method of prevention of mass gastrointestinal and respiratory diseases of suckling pigs | |
| Oliver et al. | Heifer mastitis: prevalence, risk factors and control strategies | |
| CN104434970A (en) | Medicine for treating nematodosis and trematodiasis of cattle and sheep and preparation method of medicine | |
| Cariddi et al. | Medicinal plants as immune response enhancers to prevent infectious diseases of veterinary interest | |
| Deolindo et al. | Lacaune ewes with subclinical mastitis: effects of intramammary application of própolis | |
| KR20130013965A (en) | Anti-fungal and anti-bacterial compositions against fish pathogenic bacteria | |
| US20080075799A1 (en) | Use Of Medicinal Plants And Extracts Thereof With Growth Promoting Activity In Veterinary Medicine And Zootechnics | |
| CN108815274A (en) | Clean maintenance bacteriostatic gel of woman and its preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |