CN104585060A - Pleurotus eryngii var. tuoliensis and cultivating method thereof - Google Patents
Pleurotus eryngii var. tuoliensis and cultivating method thereof Download PDFInfo
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Abstract
The invention discloses a pleurotus eryngii var. tuoliensis belonging to the field of edible fungi. The pleurotus eryngii var. tuoliensis strain CCAS NO.7 was collected in China General Microbiological Culture Collection Center at 12th, December, 2014, with the collection number of CGMCC No.10197. The invention further discloses a cultivation method of the Pleurotus eryngii var. tuoliensis strain CCAS NO.7. The cultivating method comprises the following steps of mother strain breeding, stock culturing, cultivated seed cultivating, low-temperature stimulation, fruiting management, harvesting and the like. The invention provides a new Pleurotus eryngii var. tuoliensis strain, which enriches the variety of the Pleurotus eryngii var. tuoliensis; secondly, the pleurotus eryngii var. tuoliensis strain CCAS NO.7 sporocarp has high commercial values, a cap is shaped like a steamed bun, and the Pleurotus eryngii var. tuoliensis is hard in texture, beautiful in appearance and tidy in fruiting. And the pleurotus eryngii var. tuoliensis strain CCAS NO.7 is suitable for agricultural cultivation.
Description
Technical field
The invention belongs to edible mushroom field, be specifically related to a kind of Pleuotus nebrodensis Quel, and the cultivation method of this Pleuotus nebrodensis Quel.
Background technology
Pleuotus nebrodensis Quel (Pleurotus eryngii var.tuoliensis, have another name called asafoetida mushroom, the mutation of pleurotus eryngii asafoetide, Pleurotus eryngii var. nebrodensis, the mutation of pleurotus eryngii white, wing abalone mushroom, beautiful snow Pleurotus ferulae, the white glossy ganoderma in Western Paradise, Tianshan Mountains god mushroom etc.) belong to Pleurotus (Pleurotus), Chinese formal name used at school is Pleurotus nebrodensis (or be: the mutation of pleurotus eryngii Tuoli) by Classification system Pleurotus eryngii var.tuoliensis literal translation.Pleurotus nebrodensis sporophore plumpness is pure white, quality is fine and smooth tasty and refreshing, nutritious, is a kind of very precious large edible bacterium.Pleuotus nebrodensis Quel facultative parasitism is on the basal part of stem and root of half withered Ferula sinkiangensis, and wild resource is extremely rare.
" Pleuotus nebrodensis Quel " title buys asafoetida mushroom bacterium bag from Beijing Jinxin Edible Fungi Co., Ltd. in 1997 from Xinjiang, in the success of Beijing large area fruiting, classification of fungi scholar fourth of the twelve Earthly Branches Mr. morning mist wherein literature name will be decided to be Pleurotus nebrodensis, Classification system is decided to be Pleurotus nebrodensis, and (fourth of the twelve Earthly Branches morning mist is edited to be recorded in " Macrofungi From China ", Henan science tech publishing house, 2000).But the Pleurotus nebrodensis that the Pleurotus nebrodensis of the China of research discovery is afterwards real from Europe is different, the Pleurotus nebrodensis formal name used at school of China should be the Pleurotus eryngii var.tuoliensis (.mycoscience such as Kawai, 2008,49:75 ~ 79; Huang Chenyang etc. plant genetic resources journal, 2011,12 (5): 825 ~ 827,823).And obtained authority international mycology name database (
http:// www.indexfungorum.org/) admit, namely the formal name used at school of Pleuotus nebrodensis Quel is Pleurotus eryngii var.tuoliensis.
Pleuotus nebrodensis Quel is a kind of edible Rare edible fungus all very high with medical value, and it has agriculture formula to cultivate and factory culture two kinds of planting types, and Cultivation of Pleurotus nebrodensis exists following main bugbear: (1), bacterial classification are few.At present, the Pleurotus nebrodensis strain that country is assert only has 4, and national Main Cultivation bacterial classification is estimated to be no more than 10, and wherein factory culture mainly middle peasant No. 1, agriculture formula cultivates mainly middle peasant's wing Bao.All there is shortcoming in these kinds, as on the low side in middle peasant's No. 1 biological efficiency, and fruit body is softer, not storage tolerance; Middle peasant's wing Bao cultivation period is long, and fruit body is water stain many, appearance poor.(2), latter stage of ripening, is long.Pleuotus nebrodensis Quel is the kind that typical case needs after-ripening, and latter stage of ripening, from being inoculated into, fruiting needed about 120 days usually at about 2 months.(3), biological efficiency is low.Pleuotus nebrodensis Quel cultivation cycle is oversize, and after after-ripening completes, planting material percentage of water loss is high, if not moisturizing before fruiting (as factory culture), biological efficiency only has 30% ~ 40%, if moisturizing before fruiting (as agriculture formula cultivation earthing), biological efficiency can reach more than 60%.
Summary of the invention
For the shortcoming that Pleurotus nebrodensis strain is few, the object of the invention is to provide a kind of new Pleuotus nebrodensis Quel.
Another object of the present invention is the cultivation method providing above-mentioned Pleuotus nebrodensis Quel.
Object of the present invention realizes by following technical solution:
A kind of Pleuotus nebrodensis Quel (Pleurotus eryngii var.tuoliensis) No. 7, bacterial strain middle peasant Bai Ling of the present invention, on December 12nd, 2014, in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, (preservation address was: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, postcode 100101), its deposit number is CGMCC No.10197.
The cultivation method of above-mentioned No. 7, Pleuotus nebrodensis Quel bacterial strain middle peasant Bai Ling, comprises the steps:
(1), femalely plant breeding: in the weight ratio ratio of 1:80 ~ 100 by middle peasant Bai Ling No. 7 strain inoculation of diameter 0.3 ~ 0.5cm on mother culture media, 22 ~ 28 DEG C, relative air humidity is 40 ~ 60%, cultivate 10 ~ 12 days under lucifuge condition, obtain No. 7, middle peasant Bai Ling mother and plant;
(2), Primary spawn: according to percentage by weight be 3 ~ 5% ratio No. 7, the middle peasant Bai Ling of gained in step (1) mother planted be inoculated on cultivation composts or fertilisers of cultivating, 22 ~ 28 DEG C, relative air humidity is 40 ~ 60%, cultivate 28 ~ 35 days under lucifuge condition, obtains middle peasant Bai Ling No. 7 original seeds;
(3), cultivated species is cultivated: according to percentage by weight be 3 ~ 5% ratio middle peasant Bai Ling No. 7 original seeds of gained in step (2) are inoculated on cultivation composts or fertilisers of cultivating, 22 ~ 28 DEG C, relative air humidity is 40 ~ 60%, cultivate 28 ~ 35 days under lucifuge condition, obtains middle peasant Bai Ling No. 7 cultivated speciess;
(4), cooling inoculation: according to percentage by weight be 5 ~ 8% ratio middle peasant Bai Ling No. 7 cultivated speciess of gained in step (3) are inoculated on cultivation composts or fertilisers of cultivating, 22 ~ 28 DEG C, relative air humidity is 40 ~ 60%, cultivate 30 ~ 40 days under lucifuge condition, mycelia covers with composts or fertilisers of cultivating;
(5), mycelium stimulation: opened by sack, with the mycoderma on sterilization spatula removing charge level, pine is pricked suitable for reading, is then pricked by bacterium bag pine, continues cultivation 3 ~ 5 days, until mycelia recovers;
(6), low temperature stimulation: stimulate 7 ~ 14 days under bacterium bag being placed in 1 ~ 3 DEG C of low temperature; Or greenhouse temperature is 0 ~ 15 DEG C time, keeps day and night temperature 10 ~ 15 DEG C, stimulate 10 ~ 15 days;
(7), management of producing mushroom and gathering: after there is former base, in 5 ~ 18 DEG C (agriculture formula cultivations) or 12 ~ 14 DEG C (industrial formula cultivation), relative air humidity is 75 ~ 85%, illumination 500 ~ 800 lux, well-ventilated, environment CO
2≤ 1000 × 10
-6cultivate 20 ~ 30 days under condition; When fruit body cap fully launches, but still have slight crimping, fruit body of gathering when spore does not discharge.
Bacterial classification described in above-mentioned cultivation method step (1) refers to mycelia.
Raw material constituent and the part by weight thereof of the mother culture media described in above-mentioned cultivation method step (1) are: peeled potatoes block 180 ~ 300g (getting juice), glucose 15 ~ 20g, potassium dihydrogen phosphate 0.5 ~ 3g, magnesium sulfate 0.5 ~ 3g, agar 15 ~ 25g, water 1000ml, pH value nature.
Raw material constituent and the percentage by weight thereof of above-mentioned cultivation method step (2), (3) or the cultivation composts or fertilisers of cultivating described in (4) are: cotton seed hulls 75 ~ 81%, wheat bran 8 ~ 15%, corn flour 2 ~ 8%, lime 1 ~ 3%; Be that the ratio of 1:1.8 ~ 2.3 adds water again with material-water ratio.
The constituent of the cultivation composts or fertilisers of cultivating described in above-mentioned cultivation method and percentage by weight thereof are preferably: cotton seed hulls 81%, wheat bran 12.5%, corn flour 3.5%, lime 3%, material-water ratio 1:1.86, namely water content is 65%.
Above-mentioned cultivation method step (1), (2), (3) or the temperature described in (4) are preferably 24 ~ 26 DEG C, more preferably 25 DEG C.
Above-mentioned cultivation method step (1), (2), (3) or the relative air humidity described in (4) are preferably 60%.
Inoculation method described in above-mentioned cultivation method step (4) is the hollow plastic stick of first extracting the insertion in advance of bacterium bag central authorities, in the hole stayed after hollow plastic stick is extracted in cultivated species access.
The preparation method of the mother culture media described in above-mentioned cultivation method step (1), comprises and proportionally peeled potatoes block (diameter is about 1cm) is placed in 600ml water, boiling water boiling 30min, 4 layers of filtered through gauze, taking juice; Add in gained juice and be less than 400ml water, then add glucose, potassium dihydrogen phosphate, magnesium sulfate, agar, be settled to 1000ml, little fire heating while stirring, dissolve completely to above composition, packing test tube (specification is 20mm × 200mm), 121 DEG C of sterilizing 30min; Pendulum inclined-plane, for subsequent use.Agar used can be agar strip or agar powder.
The preparation method of above-mentioned cultivation method step (2), the cultivation composts or fertilisers of cultivating described in (3) is: proportionally mixed by each raw material components, stir; Add water according to material-water ratio again, stir, dress bottle/bag, cultivation composts or fertilisers of cultivating adopts autoclaving, keeps 150min at 125 DEG C.
The preparation method of the cultivation composts or fertilisers of cultivating described in above-mentioned cultivation method step (4) is: proportionally mixed by each raw material components, stir; Add water according to material-water ratio again, stir, cultivation bag adopts polypropylene or Polythene Bag, general 5 ~ 6 of cultivation bag thickness, bacterium bag folding footpath 17cm, long 35cm; The cultivation composts or fertilisers of cultivating prepared is loaded in bag, requires degree of tightness appropriateness, install in the hole in the middle of the press-in of rear sack varus, and insert hollow bar, put in disinfection basket, namely carry out sterilizing.General employing normal-pressure sterilization, in steamer after air, keeps 12 ~ 14 hours, inoculates after cooling at 100 DEG C.
The separation domestication process of No. 7, Pleuotus nebrodensis Quel middle peasant Bai Ling of the present invention:
In May, 2009, the present inventor's Gobi desert near the brick field of Yumin County, Tacheng area collects the wild Pleurotus nebrodensis sporophore colonizing in Ferula sinkiangensis root, mycelia (bacterial classification) is obtained by fruit body tissue separation, detect mycelia rDNA-ITS size and sequence, identify that it is Pleuotus nebrodensis Quel (Pleurotus eryngii var.tuoliensis), cultivate being separated the bacterial classification obtained in 2009-2011, the annual fruit body good to mushroom shape is carried out tissue and is separated, all at difcoPDA medium, (constituent of BDdifcoPDA medium and ratio thereof are: BDdifcoPDA powder 39g, distilled water 1000ml) upper cultivation, therefrom filter out mycelial growth rate fast, robust growth, the bacterial strain of bacterium colony rounding.2012, experiment in cultivation in 2013 finds that No. 2750 bacterial strain fruiting performances filtered out for 2011 are excellent, No. 7, called after middle peasant Bai Ling.
Advantage of the present invention and beneficial effect: (1) the invention provides a kind of new Pleuotus nebrodensis Quel bacterial strain, has enriched the type of Pleuotus nebrodensis Quel; (2) cap of this bacterial strain is snowy white, average 11.9 centimetres of major diameter, average 10.5 centimetres of minor axis, average 5.2 centimetres of thickness; Raw in stem, white, upper slightly inferior, quality is hard, smooth surface, stem on average long 5.0 centimetres, on average thick 4.6 centimetres; Lamella is creamy white, and has reticulate pattern.(3) the fruit body commodity value of No. 7, middle peasant Bai Ling of the present invention is higher, and cap is steamed bun shape, and quality is hard, and outward appearance is beautiful, and fruiting is neat, and form uniformity is high.The average single mushroom of agricultural formula cultivation weighs 226.6 ± 24.3g, and every bag can go out 2 mushrooms.
Accompanying drawing illustrates:
Fig. 1. the fruit body photo of No. 7, middle peasant Bai Ling.
Fig. 2. adopt the ISSR collection of illustrative plates of 5 Pleurotus nebrodensis strains of primer P4 amplification.Wherein M is Marker, and 1 be China assorted 13,2 is middle peasant's wing Bao, and 3 is KH2, and 4 is middle peasant No. 1, and 5 is No. 7, middle peasant Bai Ling.
Fig. 3. adopt the ISSR collection of illustrative plates of 5 Pleurotus nebrodensis strains of primer P24 amplification; Wherein M is Marker, and 1 be China assorted 13,2 is middle peasant's wing Bao, and 3 is KH2, and 4 is middle peasant No. 1, and 5 is No. 7, middle peasant Bai Ling.
Fig. 4. the IGS2 that No. 7, middle peasant Bai Ling is through Hap II restriction enzyme digestion and electrophoresis collection of illustrative plates; Wherein 1 is middle peasant No. 1, and 2 is No. 7, middle peasant Bai Ling.
Fig. 5. the IGS2 that No. 7, middle peasant Bai Ling is through Rsa I restriction enzyme digestion and electrophoresis collection of illustrative plates; Wherein 1 is middle peasant No. 1, and 2 is No. 7, middle peasant Bai Ling.
Embodiment:
Below by way of example, the invention will be further described, but do not constitute any limitation the present invention.
The taxonomic identification of No. 7, embodiment 1 middle peasant Bai Ling of the present invention:
(1) morphological feature of No. 7, middle peasant Bai Ling and taxonomic identification:
Middle peasant Bai Ling No. 7 former bases are scattered, canescence; Time ripe, cap is snowy white, mussel shape, and quality is hard, average 11.9 centimetres of major diameter, average 10.5 centimetres of minor axis, average 5.2 centimetres of thickness; Raw in stem, white, upper slightly inferior, quality is hard, smooth surface, stem on average long 5.0 centimetres, on average thick 4.6 centimetres; Lamella is plain boiled pork pink colour, has reticulate pattern.Cap aspect ratio is about 1.1:1, and the aspect ratio of stem is about 1.1:1, and cap ratio that is long and stem length is about 2.4:1.According to " Macrofungi From China ", (fourth of the twelve Earthly Branches morning mist is edited, Henan science tech publishing house, within 2000, publish) the 66th page of photo about Pleuotus nebrodensis Quel and individual features describe, No. 7, middle peasant Bai Ling is consistent with the morphological feature of Pleuotus nebrodensis Quel, therefore, No. 7, middle peasant Bai Ling belongs to Pleuotus nebrodensis Quel in classification, and the Pleuotus nebrodensis Quel Classification system introduced as technical background changes, and its correct Classification system is Pleurotuseryngii var.tuoliensis.
(2) identification and analysis is contrasted with the ISSR collection of illustrative plates of 4 the Pleuotus nebrodensis Quel bacterial strains assert by country
(1), test strain: control strain: the Pleuotus nebrodensis Quel bacterial strain assert by country only has 4, i.e. assorted No. 13 of China, middle peasant's wing Bao, KH2 and middle peasant No. 1; Bacterial strain of the present invention: No. 7, middle peasant Bai Ling
(2), test method: with the DNA of bacterial strain for template, carry out ISSR amplification with P4 or P24 for primer respectively, obtain ISSR amplified production; Described primer sequence is as follows respectively:
P4：5’-GGATGCAACACACACACAC-3’(SEQ ID No.1)
P24：5’-CACGAGAGAGAGAGAGA-3’(SEQ ID No.2)
ISSR amplification system is: dNTP (2.5mM) 1.5 μ L, primer (10 μm of ol) 1 μ L, Ex Taq
tMarchaeal dna polymerase 0.5U, 10 × Ex Taq PCR buffer solution 2 μ L, DNA profiling 4 μ L, uses ddH
2o polishing to 20 μ L; ISSR reaction condition: 94 DEG C of 4min; 94 DEG C of 50s, 55 DEG C of 50s, 72 DEG C of 2min, totally 35 circulations; 72 DEG C of polishing 7min.Amplified production is carried out electrophoresis detection on 1.0% Ago-Gel, detects ISSR amplification.
Result with P4 be primer amplification ISSR collection of illustrative plates (see Fig. 2) and with P24 be primer amplification ISSR collection of illustrative plates (see Fig. 3) in all can find out that No. 7, middle peasant Bai Ling and China are assorted 13, all there is notable difference band in middle peasant's wing Bao, KH2, middle peasant No. 1, illustrates that No. 7, middle peasant Bai Ling is a kind of new Pleuotus nebrodensis Quel (Pleurotuseryngii var.tuoliensis) bacterial strain.
(3) IGS2-RFLP collection of illustrative plates is utilized to carry out identification and analysis to No. 7, middle peasant Bai Ling
Test strain: No. 7, middle peasant Bai Ling and middle peasant No. 1
Test method: (1), DNA extract: extract middle peasant Bai Ling No. 7 DNA according to a conventional method.
(2), primer: InvSR1R and 5SRNAR.
InvSR1R：5’-ACTGGCAGAATCAACCAGGTA-3’(SEQ ID No.3)
5SRNAR：5’-ACCGCATCCCGTCTGAT-3’(SEQ ID No.4)
(3), restriction enzyme: Hap II, Rsa I.
IGS2-PCR amplification system: dNTP (2.5mM each) 4 μ L, InvSR1R, 5SRNAR each 2.5 μ L, Ex Taq
tMarchaeal dna polymerase 1.25U, 1 × PCR buffer solution, template 25ng, uses ddH
2o polishing to 50 μ L.IGS2-RFLP reaction condition: 94 DEG C of 1min, 60 DEG C of 1min, 72 DEG C of 3min, totally 35 circulations; 72 DEG C, 7min.Enzyme cuts system: restriction enzyme (10U/ μ L) 1 μ L, PCR primer 6 μ L, 10 × buffer 1 μ L, with water polishing to 10 μ L.At being placed in 37 DEG C, enzyme cuts 4 hours.1.2% agarose gel electrophoresis, detects endonuclease reaction result under ultraviolet gel imaging system.Data processing adopts Quantity One analysis software.
The IGS2 of No. 7, result middle peasant Bai Ling cuts (see Fig. 4) through Hap II enzyme and generates 3 fragments that size is 609bp, 1487bp, 1821bp; Cut (see Fig. 5) through Rsa I enzyme and generate 5 fragments that size is 454bp, 510bp, 1118bp, 1838bp, 2078bp.Contrast finds that No. 7, middle peasant Bai Ling and other Pleuotus nebrodensis Quel bacterial strains all exist notable difference on banding pattern, illustrates that No. 7, middle peasant Bai Ling is a kind of new Pleuotus nebrodensis Quel bacterial strain.
The experiment in cultivation of No. 7, embodiment 2 Pleuotus nebrodensis Quel middle peasant of the present invention Bai Ling
(1) female breeding of planting: ((preservation address was: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms on December 12nd, 2014 by middle peasant Bai Ling No. 7 bacterial classifications of 0.3 ~ 0.5cm size in the weight ratio ratio of 1:80, postcode 100101), its deposit number is CGMCC No10197) be inoculated on mother culture media, 25 DEG C, relative air humidity 60%, to cultivate 12 days under lucifuge condition, cover with test tube slant, obtain No. 7, middle peasant Bai Ling mother and plant; Wherein the preparation method of mother culture media is: by peeled potatoes stripping and slicing (diameter is about 1cm) 200g, be placed in about 600ml water, boiling water boiling 30min, 4 layers of filtered through gauze, taking juice; Add in gained juice and be less than 400ml cold water, then add glucose 20g, potassium dihydrogen phosphate 2g, magnesium sulfate 0.5g, agar powder 15g, be settled to 1000ml, be stirred to agar powder without conglomeration, little fire heating is also stirred simultaneously, dissolves completely to above composition, packing test tube, 121 DEG C of sterilizing 30min; Pendulum inclined-plane, obtains mother culture media.
(2) Primary spawn: No. 7, step (1) gained middle peasant Bai Ling mother kind is inoculated on cultivation composts or fertilisers of cultivating according to the ratio of percentage by weight 5%, 25 DEG C, relative air humidity 60%, to cultivate 30 days under lucifuge condition, obtain middle peasant Bai Ling No. 7 original seeds; Described cultivation composts or fertilisers of cultivating is prepared as follows: according to percentage by weight ratio by cotton seed hulls 81%, wheat bran 12.5%, corn flour 3.5%, and lime 3% mixes, and stirs; Add water according to the part by weight of material-water ratio 1:1.86 again, stir, dress bacterium bag, bacterium bag specification is 17cm × 33cm, the Polypropylene Bag of thick 4, and pack height 10cm, rubber band tying, adopts autoclaving, keeps 150min at 125 DEG C.After the sterilizing of bacterium bag, take out spreading for cooling.
(3) cultivated species is cultivated: according to percentage by weight be 5% ratio step (2) gained middle peasant Bai Ling No. 7 original seeds are inoculated in cultivation composts or fertilisers of cultivating (its constituent and the same step of ratio (2) thereof), 25 DEG C, relative air humidity 60%, to cultivate 28 days under lucifuge condition, obtain middle peasant Bai Ling No. 7 cultivated speciess.
(4) preparation of composts or fertilisers of cultivating, is cultivated: prepare cultivation composts or fertilisers of cultivating with reference to step (2), pack, bacterium bag specification is 17cm × 35cm, the Polypropylene Bag of thick 4, expect high about 16cm, the middle edible mushroom plastic hollow rod inserting long 13cm, with the diameter 3.5cm collar and supporting tampon cap seal mouth; Adopt the sterilizing of normal pressure bacterium, keep 14 hours at 100 DEG C.
(5), cooling inoculation: cooling chamber is cleaned up rear sterilization, step (4) gained bacterium bag is put into cooling chamber and is reduced to normal temperature to temperature.Aseptically, take out the edible mushroom hollow plastic stick in bacterium bag, according to the ratio of percentage by weight 5%, middle peasant Bai Ling No. 7 cultivated speciess are inoculated in the hole of bacterium bag central authorities, with the collar and supporting tampon cap seal mouth; At 25 DEG C, relative air humidity 60%, half-light, cultivates under well-ventilated's condition and covers with bacterium bag in 30 days.
(6), mycelium stimulation: opened by sack, with the mycoderma of sterilization spatula removing charge level 3 square centimeters of sizes, pine is pricked suitable for reading, is then pricked by bacterium bag pine, continues cultivation 5 days, until mycelia recovers.
(7), low temperature stimulation: bacterium bag to be put under freezer 1 DEG C of condition low temperature stimulation 7 days.
(8), management of producing mushroom and gathering: the bacterium bag that will process through step (7), proceeds to mushroom room, control temperature at 12 DEG C, relative air humidity 85%, illumination 500 ~ 800 lux, well-ventilated, environment CO
2≤ 1000 × 10
-6cultivate 28 days under condition; When fruit body cap fully launches, but still have slight crimping, fruit body of gathering when spore does not discharge; Cut off from fruit body root when gathering, with vanning after tin foil parcel.
The cultivation period of No. 7, result middle peasant Bai Ling is 100-110 days; Agricultural formula is cultivated single mushroom and is weighed 226.6 ± 24.3g, and every bag can go out 2 mushrooms.Fruit body (see Fig. 1) commodity value of No. 7, middle peasant Bai Ling of the present invention is higher, and its cap is steamed bun shape, and color is snow-white, and quality is hard.Average 11.9 centimetres of cap major diameter, average 10.5 centimetres of minor axis, average 5.2 centimetres of thickness; Raw in stem, average long 5.0cm, average thick 4.6cm.No. 7, middle peasant Bai Ling is applicable to the cultivation of agriculture formula.
Claims (10)
1. Pleuotus nebrodensis Quel (Pleurotus eryngii var.tuoliensis) No. 7, a bacterial strain middle peasant Bai Ling, on December 12nd, 2014 in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, its deposit number is CGMCC No.10197.
2. the cultivation method of No. 3, Pleuotus nebrodensis Quel bacterial strain middle peasant Bai Ling according to claim 1, is characterized in that comprising the steps:
(1), in the weight ratio ratio of 1:80 ~ 100 by middle peasant Bai Ling No. 7 strain inoculation of diameter 0.3 ~ 0.5cm on mother culture media, 22 ~ 28 DEG C, relative air humidity is 40 ~ 60%, cultivate 10 ~ 12 days under lucifuge condition, obtain No. 7, middle peasant Bai Ling mother and plant;
(2), according to percentage by weight be 3 ~ 5% ratio No. 7, the middle peasant Bai Ling of gained in step (1) mother planted be inoculated on cultivation composts or fertilisers of cultivating, 22 ~ 28 DEG C, relative air humidity is 40 ~ 60%, cultivate 28 ~ 35 days under lucifuge condition, obtains middle peasant Bai Ling No. 7 original seeds;
(3), according to percentage by weight be 3 ~ 5% ratio middle peasant Bai Ling No. 7 original seeds of gained in step (2) are inoculated on cultivation composts or fertilisers of cultivating, 22 ~ 28 DEG C, relative air humidity is 40 ~ 60%, cultivate 28 ~ 35 days under lucifuge condition, obtains middle peasant Bai Ling No. 7 cultivated speciess;
(4), according to percentage by weight be 5 ~ 8% ratio middle peasant Bai Ling No. 7 cultivated speciess of gained in step (3) are inoculated on cultivation composts or fertilisers of cultivating, 22 ~ 28 DEG C, relative air humidity is 40 ~ 60%, cultivate 30 ~ 40 days under lucifuge condition, mycelia covers with composts or fertilisers of cultivating;
(5), by sack open, with the mycoderma on sterilization spatula removing charge level, pine is pricked suitable for reading, is then pricked by bacterium bag pine, continues cultivation 3 ~ 5 days, until mycelia recovers;
(6) stimulate 7 ~ 14 days under, bacterium bag being placed in 1 ~ 3 DEG C of low temperature; Or greenhouse temperature is 0 ~ 15 DEG C time, keeps day and night temperature 10 ~ 15 DEG C, stimulate 10 ~ 15 days;
(7), there is former base after, be 5 ~ 18 DEG C (agriculture formula cultivations) or 12 ~ 14 DEG C (industrial formula cultivation) in temperature, relative air humidity is 75 ~ 85%, illumination 500 ~ 800 lux, well-ventilated, environment CO
2≤ 1000 × 10
-6cultivate 20 ~ 30 days under condition; When fruit body cap fully launches, but still have slight crimping, fruit body of gathering when spore does not discharge.
3., according to cultivation method according to claim 2, it is characterized in that the bacterial classification described in its step (1) refers to mycelia.
4. according to cultivation method according to claim 2, it is characterized in that raw material constituent and the part by weight thereof of the mother culture media described in its step (1) are: peeled potatoes block 180 ~ 300g, glucose 15 ~ 20g, potassium dihydrogen phosphate 0.5 ~ 3g, magnesium sulfate 0.5 ~ 3g, agar 15 ~ 25g, water 1000ml, pH value nature.
5. according to cultivation method according to claim 2, it is characterized in that its step (2), the raw material constituent of (3) or the cultivation composts or fertilisers of cultivating described in (4) and percentage by weight thereof are: cotton seed hulls 75 ~ 81%, wheat bran 8 ~ 15%, corn flour 2 ~ 8%, lime 1 ~ 3%; Be that the ratio of 1:1.8 ~ 2.3 adds water again with material-water ratio.
6., according to cultivation method according to claim 5, it is characterized in that the constituent of described cultivation composts or fertilisers of cultivating and percentage by weight thereof are: cotton seed hulls 81%, wheat bran 12.5%, corn flour 3.5%, lime 3%, material-water ratio 1:1.86, namely water content is 65%.
7., according to cultivation method according to claim 2, it is characterized in that its step (1), (2), (3) or the temperature described in (4) are 24 ~ 26 DEG C.
8., according to cultivation method according to claim 7, it is characterized in that described temperature is 25 DEG C.
9., according to cultivation method according to claim 2, it is characterized in that its step (1), (2), (3) or the relative air humidity described in (4) are 60%.
10., according to cultivation method according to claim 2, it is characterized in that the inoculation method described in its step (4) is the hollow plastic stick of first extracting the insertion in advance of bacterium bag central authorities, in the hole stayed after hollow plastic stick is extracted in cultivated species access.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106993465A (en) * | 2016-01-22 | 2017-08-01 | 许昌世纪香生物科技有限公司 | Pleurotus nebrodensis liquid spawn is cultivated and Pleurotus nebrodensis bottle plants industrial production method |
| CN108934754A (en) * | 2018-06-29 | 2018-12-07 | 贵州健丰农业发展有限公司 | A kind of artificial cultivation method of Pleurotus ferulae Lanzi good quality and high output |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1648234A (en) * | 2004-10-24 | 2005-08-03 | 张红伟 | High temperature Pleuotus nebrodensis Quel Tianshan 619 and cultivating method |
| CN1692693A (en) * | 2004-10-09 | 2005-11-09 | 北京金信食用菌有限公司 | Edible bailing mushroom |
| CN1813513A (en) * | 2005-01-31 | 2006-08-09 | 中国农业科学院农业资源与农业区划研究所 | Bailing mushroom new variety, and bacterial species production and culture method |
| JP2008104380A (en) * | 2006-10-24 | 2008-05-08 | Asahimatsu Shokuhin Kk | New pleurotus eryngii var. tuoliensis strain |
| KR20140011152A (en) * | 2012-07-17 | 2014-01-28 | 권경열 | Novel pleurotus eryngii var. ferulae strain |
-
2015
- 2015-01-07 CN CN201510007502.1A patent/CN104585060B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1692693A (en) * | 2004-10-09 | 2005-11-09 | 北京金信食用菌有限公司 | Edible bailing mushroom |
| CN1648234A (en) * | 2004-10-24 | 2005-08-03 | 张红伟 | High temperature Pleuotus nebrodensis Quel Tianshan 619 and cultivating method |
| CN1813513A (en) * | 2005-01-31 | 2006-08-09 | 中国农业科学院农业资源与农业区划研究所 | Bailing mushroom new variety, and bacterial species production and culture method |
| JP2008104380A (en) * | 2006-10-24 | 2008-05-08 | Asahimatsu Shokuhin Kk | New pleurotus eryngii var. tuoliensis strain |
| KR20140011152A (en) * | 2012-07-17 | 2014-01-28 | 권경열 | Novel pleurotus eryngii var. ferulae strain |
Non-Patent Citations (3)
| Title |
|---|
| MENGRAN ZHAO, ET. AL.: "Genetic variability and population structure of the mushroom pleurotus eryngii var. tuoliensis", 《PLOS ONE》 * |
| 张瑞颖等: "白灵菇栽培经验及问题分析", 《中国食用菌》 * |
| 徐彦军等: "白灵菇栽培研究进展", 《安徽农业可科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106993465A (en) * | 2016-01-22 | 2017-08-01 | 许昌世纪香生物科技有限公司 | Pleurotus nebrodensis liquid spawn is cultivated and Pleurotus nebrodensis bottle plants industrial production method |
| CN108934754A (en) * | 2018-06-29 | 2018-12-07 | 贵州健丰农业发展有限公司 | A kind of artificial cultivation method of Pleurotus ferulae Lanzi good quality and high output |
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| CN104585060B (en) | 2016-08-24 |
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