CN104584879A - Cultivation method of auricularia polytricha - Google Patents

Cultivation method of auricularia polytricha Download PDF

Info

Publication number
CN104584879A
CN104584879A CN201510085901.XA CN201510085901A CN104584879A CN 104584879 A CN104584879 A CN 104584879A CN 201510085901 A CN201510085901 A CN 201510085901A CN 104584879 A CN104584879 A CN 104584879A
Authority
CN
China
Prior art keywords
auricularia polytricha
turning
cultivation
mushroom tang
mushroom
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201510085901.XA
Other languages
Chinese (zh)
Inventor
邬方成
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN201510085901.XA priority Critical patent/CN104584879A/en
Publication of CN104584879A publication Critical patent/CN104584879A/en
Pending legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D3/00Calcareous fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00Other organic fertilisers

Abstract

The invention discloses a cultivation method of auricularia polytricha. The method is a technology for applying lentinus edodes stem root to cultivation of auricularia polytricha. Compared with the prior art, the cultivation method of auricularia polytricha has the advantages that a lentinus edodes stem root soaking material and the cultivation raw material are mixed, stacked and fermented, the beneficial microorganism reproduction is accelerated, the microbial community in the material is improved, and the catalytic decomposition of the cultivation material is accelerated; the fermented cultivation material comprise high-activity beneficial bacteria and active metabolite of the high-activity beneficial bacteria, the acceleration effect on the growth of auricularia polytricha hyphae is achieved, the hyphae has the characteristics of strong growth and high in hybrid bacteria resistance; after the second batch of auricularia polytricha and the third batch of auricularia polytricha are harvested, a good effect of re-strengthening the hyphae by using the lentinus edodes stem root leaching liquid is achieved, an acceleration effect on the formation of auricularia polytricha base and the growth of the auricularia polytricha is achieved, and the third batch of auricularia polytricha and the fourth batch of auricularia polytricha are high in biotransformation efficiency; the resources such as labor and carbon are economized; the pollution to the environment is reduced; the low-carbon environmental protection concept can be met.

Description

A kind of Uricularia polytricha cultivation method
Technical field
A kind of Uricularia polytricha cultivation method of the present invention, is mushroom tang is applied to the technology in Uricularia polytricha cultivation, belongs to fungus growing technique field.
Background technology
Uricularia polytricha quality is tender and crisp, and containing higher carbohydrate, protein, multivitamin and each seed amino acid, nutritious, delicious flavour, is called as " umbrella of jellyfish on tree ".
The traditional cultivation method of Uricularia polytricha is cured material bag-cultured, and planting material carries out inoculated and cultured after high pressure or normal-pressure sterilization, and the contour molecular organic material of the cellulose now in material is degraded, miscellaneous bacteria in material is also killed, therefore, when planting material sends out bacterium, mycelial growth is fast, and living contaminants is few.But along with the significantly rise of the price of labour power and fuel price, grog cultivation cost increases severely, and high-temperature sterilization also causes the loss of planting material nutriment, causes planting material conversion ratio to reduce simultaneously.
Mushroom is famous and precious edible one of the medicinal fungus of holding concurrently in the world, and current China mushroom production accounts for 70% of world's mushroom gross yield, has the equal mushroom culture in the province of more than 70% in China.Because mushroom stems stiffness of foot in children, content of cellulose are higher, palatability is poor, and therefore, mushroom is being eaten raw or in process, the tang of mushroom can remove as offcuts by the producer, causes the mushroom tang accounting for mushroom weight about 30% to become discarded object.
Summary of the invention
For the deficiencies in the prior art, the present invention adds the immersion of mushroom tang and expects to mix and stir to carry out banking up with culturing raw material to ferment, and do not add insecticide, bactericide during windrow, the planting material after fermentation is directly taped against ridge-up bed cultivation, and the Uricularia polytricha quality of producing is good, output is high.
The technical solution adopted in the present invention is:
A kind of Uricularia polytricha cultivation method, comprises batching, ferments, makes the bed, sows, hair bacterium, earthing, goes out syrinx and manage, concrete operation step:
(1) prepare burden: cotton seed hulls 76-84%, soya-bean cake 12-14%, mushroom tang 3-4%, lime 2-3%, gypsum 1%, be all the quality of dry matter, proportioning sum is 100%; Described mushroom tang is the offcuts in Lentnus edodes process, is ground into the particle of 2mm-3mm after drying;
(2) ferment: mushroom tang particle is added clear water by 20-30 weight ratio doubly, soak 18h-24h at normal temperatures, then leachate is admixed in cotton seed hulls, soya-bean cake compound uniformly together with mushroom stems slag, stockpile is become high 1.0m-1.2m, wide 2m-3m, the trapezoidal heap that length is not limit, the composts or fertilisers of cultivating often piled controls at dry weight 300kg-500kg, after carrying out heap, surrounding is patted, on heap, insert some vertical pores with the wooden stick of diameter 10cm-15cm again, at the bottom of straight through reactor, stomatal frequency is 1/m 3, finally cover straw mat by its spontaneous fermentation;
(3) turning: after material temperature reaches 60 DEG C, keeps 24h, then carries out turning, and turning every day later once, add lime during first time turning, add gypsum during second time turning, altogether turning 3-4 time, time remaining 5d-6d, when stopping fermentation, the water content of planting material is 63%-65%;
(4) make the bed, sow, send out bacterium, earthing: the planting material fermented, after heat radiation, is directly taped against ridge-up bed sowing and sends out a bacterium, when mycelia sends out material full, covers the loam of grogs diameter 0.5-1.0cm, thickness 1-1.5cm, carries out syrinx reason;
(5) go out syrinx reason: after the second damp ear, the 3rd damp ear are gathered, depending on mycelial growth situation, spray the mushroom tang leachate of 0.01-0.02g/ml in ear bed, every square metre is sprayed 4-6kg, with rejuvenation mycelia comprehensively; The preparation method of described leachate is: mushroom tang particle is added clear water by the weight ratio of 20 times, soak 18h-24h at normal temperatures, filter and remove residue, then complement to the original water yield, obtain the mushroom tang leachate that concentration is 0.05g/ml, add clear water dilution and use.
Mushroom tang is applied to the method for Uricularia polytricha cultivation by the present invention, and apart from outside explanation, other operating procedure is all undertaken by this area routine techniques.
The present invention's advantage is compared with prior art:
1. mushroom tang leachate is full-natural nutritive liquid, containing glucide, amino acid, peptide class, nucleic acid and trace element, bio-hormone also containing abundant species, also the bioactivator do not leached in a large number is had in mushroom stems slag, add and expect to mix and stir to carry out banking up with culturing raw material to ferment with the immersion of mushroom tang, promote beneficial microbe breeding, improve the microorganism species in material, accelerate the catalytic decomposition of planting material;
2. the planting material after fermentation has high activity beneficial bacterium and active metabolite thereof, has Promotive effect, show as prosperous, the anti-miscellaneous bacteria ability of mycelium growth vigor strong to the growth of Uricularia polytricha mycelia;
3., after the second damp ear, the 3rd damp ear are gathered, effective with mushroom tang leachate rejuvenation mycelia, have facilitation to the formation of Uricularia polytricha ear base and the growth of auricle, the biological transformation ratio of third and fourth damp ear is high;
4. save the resource such as manpower, coal, decrease environmental pollution, meet low-carbon environment-friendly theory.
Embodiment
Below in conjunction with embodiment, the invention will be further described, and what do not illustrate in detail in embodiment is all state of the art.
Embodiment 1: a kind of Uricularia polytricha cultivation method, comprise batching, ferment, make the bed, sow, hair bacterium, earthing, go out syrinx reason, concrete operation step:
1. prepare burden: cotton seed hulls 80%, soya-bean cake 13%, mushroom tang 3.5%, lime 2.5%, gypsum 1%, be all the quality of dry matter, proportioning sum is 100%;
2. ferment: mushroom tang particle is added clear water by 20-30 weight ratio doubly, soak 18h-24h at normal temperatures, then leachate is admixed in cotton seed hulls through prewetting, soya-bean cake compound together with mushroom stems slag uniformly, stockpile is become high 1.2m-1.5m, wide 2m-3m, the trapezoidal heap that length is not limit, the composts or fertilisers of cultivating often piled controls at dry weight 300kg-500kg, after carrying out heap, surrounding is patted, on heap, insert some vertical pores with the wooden stick of diameter 10cm-15cm again, at the bottom of straight through reactor, stomatal frequency is 1/m 3, finally cover straw mat by its spontaneous fermentation;
3. turning: after material temperature reaches 60 DEG C, keeps 24h, then carries out turning, and turning every day later once, add lime during first time turning, add gypsum during second time turning, altogether turning 3 times, time remaining 5d, when stopping fermentation, the water content of planting material is 63%-65%;
4. make the bed, sow, send out bacterium, earthing: the planting material fermented, after heat radiation, is directly taped against ridge-up bed sowing and sends out a bacterium, when mycelia sends out material full, covers the loam of grogs diameter 0.5-1.0cm, thickness 1-1.5cm, carries out syrinx reason;
5. go out syrinx reason: after having adopted the second damp ear, spray the mushroom tang leachate of 0.01g/ml in ear bed, every square metre is sprayed 4kg; After having adopted the 3rd damp ear, spray the mushroom tang leachate of 0.02g/ml, every square metre is sprayed 6kg, and by mushroom tang leachate rejuvenation mycelia, go out ear effective, the biological efficiency of third and fourth damp ear reaches 98.7%, and total biological efficiency reaches 225.1%.
Embodiment 2: a kind of Uricularia polytricha cultivation method, comprise batching, ferment, make the bed, sow, hair bacterium, earthing, go out syrinx reason, concrete operation step:
1. prepare burden: cotton seed hulls 80%, soya-bean cake 13%, mushroom tang 3.5%, lime 2.5%, gypsum 1%, be all the quality of dry matter, proportioning sum is 100%;
2. ferment: mushroom tang particle is added clear water by 20-30 weight ratio doubly, soak 18h-24h at normal temperatures, then leachate is admixed in cotton seed hulls through prewetting, soya-bean cake compound together with mushroom stems slag uniformly, stockpile is become high 1.2m-1.5m, wide 2m-3m, the trapezoidal heap that length is not limit, the composts or fertilisers of cultivating often piled controls at dry weight 300kg-500kg, after carrying out heap, surrounding is patted, on heap, insert some vertical pores with the wooden stick of diameter 10cm-15cm again, at the bottom of straight through reactor, stomatal frequency is 1/m 3, finally cover straw mat by its spontaneous fermentation;
3. turning: after material temperature reaches 60 DEG C, keeps 24h, then carries out turning, and turning every day later once, add lime during first time turning, add gypsum during second time turning, altogether turning 3 times, time remaining 5d, when stopping fermentation, the water content of planting material is 63%-65%;
4. make the bed, sow, send out bacterium, earthing: the planting material fermented, after heat radiation, is directly taped against ridge-up bed sowing and sends out a bacterium, when mycelia sends out material full, covers the loam of grogs diameter 0.5-1.0cm, thickness 1-1.5cm, carries out syrinx reason;
5. syrinx reason is gone out: after having adopted the second damp ear, the clear water of every square metre of sprinkling 4kg; After having adopted the second damp ear, the clear water of every square metre of sprinkling 6kg, the biological efficiency of third and fourth damp ear has been 67.9%, and total biological efficiency is 194.3%.
Embodiment 3: a kind of Uricularia polytricha cultivation method, comprise batching, ferment, make the bed, sow, hair bacterium, earthing, go out syrinx reason, concrete operation step:
1. prepare burden: cotton seed hulls 80%, soya-bean cake 13%, mushroom tang 3.5%, lime 2.5%, gypsum 1%, be all the quality of dry matter, proportioning sum is 100%;
2. ferment: mushroom tang particle is added clear water by 20-30 weight ratio doubly, soak 18h-24h at normal temperatures, then the leachate after removing slag is admixed uniformly in the cotton seed hulls through prewetting, soya-bean cake compound, stockpile is become high 1.2m-1.5m, wide 2m-3m, the trapezoidal heap that length is not limit, the composts or fertilisers of cultivating often piled controls at dry weight 300kg-500kg, after carrying out heap, surrounding is patted, on heap, insert some vertical pores with the wooden stick of diameter 10cm-15cm again, at the bottom of straight through reactor, stomatal frequency is 1/m 3, finally cover straw mat by its spontaneous fermentation;
3. turning: after material temperature reaches 60 DEG C, keeps 24h, then carries out turning, and turning every day later once, add lime during first time turning, add gypsum during second time turning, altogether turning 3 times, time remaining 5d, when stopping fermentation, the water content of planting material is 63%-65%;
4. make the bed, sow, send out bacterium, earthing: the planting material fermented, after heat radiation, is directly taped against ridge-up bed sowing and sends out a bacterium, when mycelia sends out material full, covers the loam of grogs diameter 0.5-1.0cm, thickness 1-1.5cm, carries out syrinx reason;
5. go out syrinx reason: after having adopted the second damp ear, spray the mushroom tang leachate of 0.01g/ml in ear bed, every square metre is sprayed 4kg; After having adopted the 3rd damp ear, spray the mushroom tang leachate of 0.02g/ml, every square metre is sprayed 6kg, and by mushroom tang leachate rejuvenation mycelia, go out ear effective, the biological efficiency of third and fourth damp ear reaches 90.1%, and total biological efficiency reaches 206.4%.
Embodiment 4: a kind of Uricularia polytricha cultivation method, comprise batching, ferment, make the bed, sow, hair bacterium, earthing, go out syrinx reason, concrete operation step:
1. prepare burden: cotton seed hulls 80%, soya-bean cake 13%, mushroom tang 3.5%, lime 2.5%, gypsum 1%, be all the quality of dry matter, proportioning sum is 100%;
2. ferment: mushroom tang particle is added clear water by 20-30 weight ratio doubly, soak 18h-24h at normal temperatures, then the leachate after removing slag is admixed uniformly in the cotton seed hulls through prewetting, soya-bean cake compound, stockpile is become high 1.2m-1.5m, wide 2m-3m, the trapezoidal heap that length is not limit, the composts or fertilisers of cultivating often piled controls at dry weight 300kg-500kg, after carrying out heap, surrounding is patted, on heap, insert some vertical pores with the wooden stick of diameter 10cm-15cm again, at the bottom of straight through reactor, stomatal frequency is 1/m 3, finally cover straw mat by its spontaneous fermentation;
3. turning: after material temperature reaches 60 DEG C, keeps 24h, then carries out turning, and turning every day later once, add lime during first time turning, add gypsum during second time turning, altogether turning 3 times, time remaining 5d, when stopping fermentation, the water content of planting material is 63%-65%;
4. make the bed, sow, send out bacterium, earthing: the planting material fermented, after heat radiation, is directly taped against ridge-up bed sowing and sends out a bacterium, when mycelia sends out material full, covers the loam of grogs diameter 0.5-1.0cm, thickness 1-1.5cm, carries out syrinx reason;
5. syrinx reason is gone out: after having adopted the second damp ear, the clear water of every square metre of sprinkling 4kg; After having adopted the 3rd damp ear, the clear water of every square metre of sprinkling 6kg, the biological efficiency of third and fourth damp ear has been 64.3%, and total biological efficiency is 180.6%.
table one Uricularia polytricha biological efficiency compares
As can be seen from Table I: the ferment effect utilizing mushroom tang leachate to add than single leachate together with the ferment effect that mushroom stems slag adds is good, and output is high; Leachate is good to the effect of rejuvenation of Uricularia polytricha mycelia, and third and fourth damp ear biological efficiency is high, obvious effect of increasing production.

Claims (1)

1. a Uricularia polytricha cultivation method, comprise batching, ferment, make the bed, sow, hair bacterium, earthing, go out syrinx reason, it is characterized in that:
(1) prepare burden: cotton seed hulls 76-84%, soya-bean cake 12-14%, mushroom tang 3-4%, lime 2-3%, gypsum 1%, be all the quality of dry matter, proportioning sum is 100%; Described mushroom tang is the offcuts in Lentnus edodes process, is ground into the particle of 2mm-3mm after drying;
(2) ferment: mushroom tang particle is added clear water by 20-30 weight ratio doubly, soak 18h-24h at normal temperatures, then leachate is admixed in cotton seed hulls, soya-bean cake compound uniformly together with mushroom stems slag, stockpile is become high 1.0m-1.2m, wide 2m-3m, the trapezoidal heap that length is not limit, the composts or fertilisers of cultivating often piled controls at dry weight 300kg-500kg, after carrying out heap, surrounding is patted, on heap, insert some vertical pores with the wooden stick of diameter 10cm-15cm again, at the bottom of straight through reactor, stomatal frequency is 1/m 3, finally cover straw mat by its spontaneous fermentation;
(3) turning: after material temperature reaches 60 DEG C, keeps 24h, then carries out turning, and turning every day later once, add lime during first time turning, add gypsum during second time turning, altogether turning 3-4 time, time remaining 5d-6d, when stopping fermentation, the water content of planting material is 63%-65%;
(4) make the bed, sow, send out bacterium, earthing: the planting material fermented, after heat radiation, is directly taped against ridge-up bed sowing and sends out a bacterium, when mycelia sends out material full, covers the loam of grogs diameter 0.5-1.0cm, thickness 1-1.5cm, carries out syrinx reason;
(5) go out syrinx reason: after the second damp ear, the 3rd damp ear are gathered, depending on mycelial growth situation, spray the mushroom tang leachate of 0.01-0.02g/ml in ear bed, every square metre is sprayed 4-6kg comprehensively; The preparation method of described leachate is: mushroom tang particle is added clear water by the weight ratio of 20 times, soak 18h-24h at normal temperatures, filter and remove residue, then complement to the original water yield, obtain the mushroom tang leachate that concentration is 0.05g/ml, add clear water dilution and use.
CN201510085901.XA 2015-02-22 2015-02-22 Cultivation method of auricularia polytricha Pending CN104584879A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510085901.XA CN104584879A (en) 2015-02-22 2015-02-22 Cultivation method of auricularia polytricha

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201510085901.XA CN104584879A (en) 2015-02-22 2015-02-22 Cultivation method of auricularia polytricha

Publications (1)

Publication Number Publication Date
CN104584879A true CN104584879A (en) 2015-05-06

Family

ID=53111223

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201510085901.XA Pending CN104584879A (en) 2015-02-22 2015-02-22 Cultivation method of auricularia polytricha

Country Status (1)

Country Link
CN (1) CN104584879A (en)

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101575239A (en) * 2008-05-05 2009-11-11 孙伟 Method for preparing auricularia polytricha culture material
CN103262753A (en) * 2013-05-23 2013-08-28 邬金飞 Auricularia polytricha soil-covered cultivation technology
CN103274775A (en) * 2013-05-20 2013-09-04 邬金飞 Auricularia polytricha cultivation material and preparation method of cultivation material
CN103420712A (en) * 2013-07-03 2013-12-04 邬金飞 Auricularia polytricha cultivation material compatibility and manufacturing method thereof
CN103435399A (en) * 2013-08-02 2013-12-11 邬金飞 Prescription of auricularia polytricha cultivation material and manufacturing method of cultivation material
CN103449895A (en) * 2013-07-11 2013-12-18 华中农业大学 Culture material for cultivating auricularia polytricha, and preparation method thereof
CN103497024A (en) * 2013-10-23 2014-01-08 邬方成 Method for preparing auricularia polytricha cultivation material through utilization of pecan production and processing waste
CN104140313A (en) * 2013-05-09 2014-11-12 李艳莉 Formula of culture material for cultivation of auricularia polytricha and preparation method thereof

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101575239A (en) * 2008-05-05 2009-11-11 孙伟 Method for preparing auricularia polytricha culture material
CN104140313A (en) * 2013-05-09 2014-11-12 李艳莉 Formula of culture material for cultivation of auricularia polytricha and preparation method thereof
CN103274775A (en) * 2013-05-20 2013-09-04 邬金飞 Auricularia polytricha cultivation material and preparation method of cultivation material
CN103262753A (en) * 2013-05-23 2013-08-28 邬金飞 Auricularia polytricha soil-covered cultivation technology
CN103420712A (en) * 2013-07-03 2013-12-04 邬金飞 Auricularia polytricha cultivation material compatibility and manufacturing method thereof
CN103449895A (en) * 2013-07-11 2013-12-18 华中农业大学 Culture material for cultivating auricularia polytricha, and preparation method thereof
CN103435399A (en) * 2013-08-02 2013-12-11 邬金飞 Prescription of auricularia polytricha cultivation material and manufacturing method of cultivation material
CN103497024A (en) * 2013-10-23 2014-01-08 邬方成 Method for preparing auricularia polytricha cultivation material through utilization of pecan production and processing waste

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
何瑞峰: "食用菌下脚料的综合利用", 《今日科技》 *
刘振祥等: "《食用菌栽培技术》", 31 July 2007 *
李艳莉等: "无公害高温双孢菇生产栽培技术规程", 《中国果菜》 *
王谦等: "以香菇柄为主料固态发酵茯苓菌", 《食品科技》 *

Similar Documents

Publication Publication Date Title
CN104541995A (en) Mushroom cultivation method
CN104541996A (en) Pholiota nameko culture method
CN104737799A (en) Auricularia polytricha cultivating method
CN104756757A (en) Method for planting hericium erinaceus
CN104823686A (en) Tremella cultivation method
CN104737798A (en) Auricularia polytricha cultivating method
CN104584869A (en) Hericium erinaceus cultivation method
CN104737778A (en) Pleurotus geesteranus cultivation method
CN104604531A (en) Cultivation method of Pholiota nameko
CN104541994A (en) Pleurotus nebrodensis culture method
CN104604529A (en) Cultivation method of Pholiota nameko
CN104782398A (en) Cultivating method for pleurotus citrinopileatus sing
CN104604534A (en) Pleurotus citrinopileatus cultivation method
CN104737807A (en) Pleurotus geesteranus cultivation method
CN104737808A (en) Pleurotus geesteranus cultivation method
CN104737803A (en) Cultivation method for auricularia auricula-judae
CN104584877A (en) Pleurotus citrinopileatus cultivation method
CN104541992A (en) Abalone mushroom culture method
CN104584879A (en) Cultivation method of auricularia polytricha
CN104737801A (en) Auricularia polytricha cultivating method
CN104541991A (en) Auricularia polytricha cultivation method
CN104737776A (en) Pleurotus eryngii cultivation method
CN104737781A (en) Pleurotus citrinopileatus cultivating method
CN104604530A (en) Cultivation method of Pholiota nameko
CN104737793A (en) Cultivation method for pholiota nameko

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20150506

RJ01 Rejection of invention patent application after publication