CN104560402A - Tricholoma matsutake extract oil and preparation method thereof - Google Patents
Tricholoma matsutake extract oil and preparation method thereof Download PDFInfo
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- CN104560402A CN104560402A CN201410856225.7A CN201410856225A CN104560402A CN 104560402 A CN104560402 A CN 104560402A CN 201410856225 A CN201410856225 A CN 201410856225A CN 104560402 A CN104560402 A CN 104560402A
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/022—Refining
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
- A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
- A23D9/02—Other edible oils or fats, e.g. shortenings, cooking oils characterised by the production or working-up
- A23D9/04—Working-up
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/025—Recovery by solvent extraction
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/027—Recovery of volatiles by distillation or stripping
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- Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Wood Science & Technology (AREA)
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- Food Science & Technology (AREA)
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- Coloring Foods And Improving Nutritive Qualities (AREA)
- Edible Oils And Fats (AREA)
Abstract
The invention discloses a tricholoma matsutake extract oil which is prepared by using aqueous enzymatic method combined with microwave low temperature vacuum extraction. The invention further discloses a tempered oil containing tricholoma matsutake extract oil component, to be specific, 1-2% of tricholoma matsutake extract oil, 70-87% of olive oil, 10-25% of deep sea fish oil and 2-3% of yak oil. The tempered oil not only has unique flavor of tricholoma matsutake, but also contains a plurality of nutrient substances, the ratio of saturated fatty acid (SFA) to monounsaturated fatty acid (MUFA) to polyunsaturated fatty acid (PUFA) is 0.3-0.5 to 1.1-1.4 to 1, and the ratio of omega-6 to omega-3 in polyunsaturated fatty acid is 4.4-5.0 to 1, so that the tempered oil not only conforms to tastes of modern consumers, but also can meet the healthy diet standard , and has the functions of regulating blood lipids and lowering cholesterol levels.
Description
Technical field
The present invention relates to edible fungi oil field, particularly relate to a kind of matsutake and extract oil and preparation method thereof.
Background technology
Along with the increase of China's hypertension, fat chronic diseases patient, chronic disease causes the attention of people.Expert points out, chronic disease is relevant with the incorrect use of eating habit (particularly edible oil) with the dietary structure of people.Angiocardiopathy is as first of chronic disease, and increasing cardiovascular patient tends to the edible oil selecting applicable own health." Chinese residents dietary int ake Dietary Reference Intakes " that Chinese Soclety of Nutrition publishes for 2000 has been formulated Chinese residents dietary fat and has been suitable for intake (AI), wherein the ratio of saturated fatty acid (SFA), monounsaturated fatty acids (MUFA), polyunsaturated fatty acid (PUFA) is 1:1:1, and in polyunsaturated fatty acid, ω-6 is 4 ~ 6:1 with the ratio of ω-3.On the other hand, along with the raising of people's living standard, consumer is while emphasis nutrient health, also fragrance and the mouthfeel of edible oil is pursued, but the part edible oil of market today is in order to improve mouthfeel or tart up, with the addition of the chemical composition of some Prof. Du Yucang, do not meet the theory of health diet.
Olive oil, in pistac, be that current medical circle is known as one of edible oil benefiting health most, the western countries such as the U.S., Germany are loaded into pharmacopeia olive oil already.Olive fruit (butt) oil-containing 35% ~ 70%, wherein pulp oil-containing 60% ~ 80%, kernel oil-containing about 30%.Olive oil is nutritious, " liquid golden ", " vegetable oil queen ", " Mediterranean sweet dew " is described as in west, it contains the monounsaturated fatty acids of 65.8 ~ 84.9%, the polyunsaturated fatty acid of 3.5 ~ 22%, polyunsaturated fatty acid can be divided into again omega-fatty acid and ω-6 aliphatic acid, digestibility, up to 100%, has very high biological value.In addition, abundant micro-squalene, Flavonoid substances, polyphenolic substance, carrotene, vitamin E and multiple liposoluble vitamin is contained in olive oil.Olive oil stimulating circulation, improve digestive system function, protection skin, improve internal system function, strengthen in skeletal system and all have good effect, can also give protection against cancer, radiation proof, anti-ageing, prevention cardiovascular and cerebrovascular disease.
Aliphatic acid in deep sea fish oil is different from the composition of land animal and plant fat, and in the vegeto-animal grease in land, aliphatic acid is generally with C
16and C
18be main, the aliphatic acid of deep sea fish oil is then with C
20and C
22polyunsaturated fatty acid is maximum.Research shows, deep sea fish oil is rich in polyunsaturated fatty acid, and being mainly ω-3 is polyunsaturated fatty acid, comprises EPA and DHA.EPA and DHA is two kinds of important functional Chinese medicines in aliphatic acid, and belonging to the mankind can not the necessary aliphatic acid of self synthesis.Medical science and the Nutritional studies of nearly more than two decades show: EPA, DHA have treatments such as suppressing platelet aggregation, antithrombotic, vasodilator, adjustment blood fat, the HDL (HDL-C) raised in blood, the LDL (LDL-C) reduced in blood and prevent and treat the function of cardiovascular and cerebrovascular diseases; Also good curative effect is had to diabetes, inflammation, ephrosis and cancer.Recently, find that again DHA also has and promote that brain cell grows, grows, improves cerebral function, improve memory and learning ability, enhancing retinal reflex ability and prevent and treat the function that senile dementia etc. improves quality of life.
Growth eats pure natural herbage the yak on plateau, there is provided the natural green food that modern advocates, its yak oil nutritive value is very high, is highly profitable to health, meanwhile, add a small amount of yak oil and can make up the saturated fatty acid composition lacked in vegetable oil.
Matsutake is the natural nourishing category of globalization, and be described as " king in bacterium ", industry mainly concentrates on Europe and Japan, is domesticly still in the starting stage.Matsutake not only as the dish of good to eat delicious food, and can have plurality kinds of health care and medical value.It is documented, matsutake has keeps fit, tonifying kidney and strengthening yang, beneficial stomach, regulates the flow of vital energy, reduces phlegm and the effect such as expelling parasite.Matsutake is not only balanced in nutrition, sufficient, but also be improved immunity, anticancer antitumor, treatment diabetes and angiocardiopathy, short stomach protects the liver the multiple efficacies such as dirty.Show according to the study, matsutake has peculiar fragrance taste, and its pleasant fragrance and agreeable to the taste fragrance are mainly from matsutake oil.The research of the Fructificatio Tricholomatis Matsutakis extract of Amino UP Chemical Co., Ltd. of Japan exploitation shows, the chemical composition of matsutake has 1-OCOL, 2-matsutake alcohol, atoxopyrimidine, carboxyl albumen, also have firpene and methyl cinnamate etc., wherein 1-OCOL is the main component of matsutake perfume (or spice).
Tricholoma oil and preparation method thereof disclosed in existing document, it mixes obtained with vegetable oil by the weight ratio of 1:3 ~ 5 by matsutake essential oil, the method adopts supercritical carbon dioxide extraction method extraction matsutake, but its equipment is complicated, conditional request is high, be difficult to suitability for industrialized production, in addition, this oil only mixes obtained with vegetable oil, constituent is single.
Summary of the invention
The object of the present invention is to provide a kind of matsutake to extract oil, this oil has unique matsutake fragrance, and pure flavor, has good commercial application prospect.
In order to realize object of the present invention, matsutake of the present invention extracts oil, obtains according to the following steps:
A) carry out ultra micro air-flow crushing by after matsutake freeze drying, cross 100 ~ 300 mesh sieves;
B) the matsutake powder of 1 weight portion is got, add the distilled water of 10 ~ 20 weight portions, add the chitinase of 0.01 ~ 0.1 weight portion again: cellulase: the mixed enzyme stirring and dissolving of papain=5:2:3, enzymolysis 2 ~ 8h under the condition of vacuum-0.02 ~-0.08MPa and temperature 45 ~ 55 DEG C;
C) by sample microwave radiation exaraction 2 ~ 4h under the condition of temperature 80 ~ 95 DEG C;
D) sample is centrifugal, filter, after filtrate organic solvent extraction, isolate aqueous phase with separatory funnel, organic solvent is removed with Rotary Evaporators reduced pressure concentration by its organic phase at 50 ~ 60 DEG C, obtains matsutake and extracts oil.
In some embodiments, above-mentioned organic solvent is food-grade ethyl acetate.
Another object of the present invention is to provide a kind of ready-mixed oil, extracts oil containing above-mentioned matsutake.
In some embodiments, also oily containing olive oil, deep sea fish oil and yak in ready-mixed oil.
In other embodiments, each component of ready-mixed oil and mass ratio are that matsutake extracts oil 1 ~ 2%, olive oil 70 ~ 87%, deep sea fish oil 10 ~ 25%, yak oil 2 ~ 3%.
Another object of the present invention is to provide a kind of preparation method of ready-mixed oil, prepared by following steps: olive oil is heated to 60 ~ 80 DEG C, add deep sea fish oil and yak oil, mixing is filtered, then adds matsutake extraction oil, stirs with the speed of 80 ~ 120r/min, abundant mixing, filter, filling, obtained ready-mixed oil finished product.
Beneficial effect of the present invention:
1, ready-mixed oil of the present invention has unique matsutake fragrance, pure flavor, the ratio of its saturated fatty acid (SFA), monounsaturated fatty acids (MUFA), polyunsaturated fatty acid (PUFA) is 0.3 ~ 0.5:1.1 ~ 1.4:1, in polyunsaturated fatty acid, ω-6 is 4.4 ~ 5.0:1 with the ratio of ω-3, both modern consumer taste had been met, meet again the standard of health diet, there is adjusting blood lipid simultaneously, reduce the effects such as cholesterol.
2, method of the present invention is by after olive oil, deep sea fish oil and the mixing of yak oil, then adds matsutake extraction oil, and it is good that matsutake extracts the dissolubility of oil in miscella, gained ready-mixed oil stable in properties.Ready-mixed oil is in storage after 20 months, and its nutrient composition content does not change, and fragrance and mouthfeel are still good.
Detailed description of the invention
Embodiment 1
A) carry out ultra micro air-flow crushing by after matsutake drying, cross 100 mesh sieves;
B) the matsutake powder of 1 weight portion is got, add the distilled water of 10 weight portions, add the chitinase of 0.1 weight portion again: cellulase: the mixed enzyme stirring and dissolving of papain=5:2:3, enzymolysis 6h under the condition of vacuum-0.08MPa and temperature 45 C;
C) by sample microwave radiation exaraction 3h under the condition of microwave power 500W and temperature 95 DEG C after, cell membrane is destroyed further;
D) sample is centrifugal, filter, filtrate, with after food-grade extraction into ethyl acetate, isolates aqueous phase with separatory funnel, and food-grade ethyl acetate is removed with Rotary Evaporators reduced pressure concentration by its organic phase at 60 DEG C, obtains matsutake and extracts oil.
Get matsutake and extract oil 1%, olive oil 82%, deep sea fish oil 15%, yak oil 2%, is first heated to 60 DEG C by olive oil, in the ratio determined, add deep sea fish oil and yak oil, mixing is filtered, then adds matsutake extraction oil, stirs with the speed of 80r/min, abundant mixing, filter, filling, obtained ready-mixed oil finished product.
Through aliphatic acid composition measuring, the ratio of SFA, MUFA, PUFA contained by this ready-mixed oil is that in 0.4:1.4:1, PUFA, ω-6 is 4.6:1 with the ratio of ω-3.
Embodiment 2
A) carry out ultra micro air-flow crushing by after matsutake freeze drying, cross 300 mesh sieves;
B) the matsutake powder of 1 weight portion is got, add the distilled water of 20 weight portions, add the chitinase of 0.01 weight portion again: cellulase: the mixed enzyme stirring and dissolving of papain=5:2:3, enzymolysis 8h under the condition of vacuum-0.02MPa and temperature 55 DEG C;
C) by sample microwave radiation exaraction 2h under the condition of microwave power 500W and temperature 80 DEG C after, cell membrane is destroyed further;
D) sample is centrifugal, filter, filtrate, with after food-grade extraction into ethyl acetate, isolates aqueous phase with separatory funnel, and food-grade ethyl acetate is removed with Rotary Evaporators reduced pressure concentration by its organic phase at 50 DEG C, obtains matsutake and extracts oil.
Get matsutake and extract oil 2%, olive oil 75%, deep sea fish oil 20%, yak oil 3%, is first heated to 80 DEG C by olive oil, in the ratio determined, add deep sea fish oil and yak oil, mixing is filtered, then adds matsutake extraction oil, stirs with the speed of 120r/min, abundant mixing, filter, filling, obtained ready-mixed oil finished product.
Through aliphatic acid composition measuring, the ratio of SFA, MUFA, PUFA contained by this ready-mixed oil is that in 0.4:1.3:1, PUFA, ω-6 is 4.9:1 with the ratio of ω-3.
Embodiment 3
A) carry out ultra micro air-flow crushing by after matsutake freeze drying, cross 200 mesh sieves;
B) the matsutake powder of 1 weight portion is got, add the distilled water of 15 weight portions, add the chitinase of 0.05 weight portion again: cellulase: the mixed enzyme stirring and dissolving of papain=5:2:3, enzymolysis 2h under the condition of vacuum-0.08MPa and temperature 55 DEG C;
C) by sample microwave radiation exaraction 4h under the condition of microwave power 500W and temperature 85 DEG C after, cell membrane is destroyed further;
D) sample is centrifugal, filter, filtrate, with after food-grade extraction into ethyl acetate, isolates aqueous phase with separatory funnel, and food-grade ethyl acetate is removed with Rotary Evaporators reduced pressure concentration by its organic phase at 55 DEG C, obtains matsutake and extracts oil.
Get matsutake and extract oil 1%, olive oil 87%, deep sea fish oil 10%, yak oil 2%, is first heated to 70 DEG C by olive oil, in the ratio determined, add deep sea fish oil and yak oil, mixing is filtered, then adds matsutake extraction oil, stirs with the speed of 100r/min, abundant mixing, filter, filling, obtained ready-mixed oil finished product.
Through aliphatic acid composition measuring, the ratio of SFA, MUFA, PUFA contained by this ready-mixed oil is that in 0.3:1.3:1, PUFA, ω-6 is 4.4:1 with the ratio of ω-3.
Embodiment 4
A) carry out ultra micro air-flow crushing by after matsutake freeze drying, cross 100 ~ 300 mesh sieves;
B) the matsutake powder of 1 weight portion is got, add the distilled water of 20 weight portions, add the chitinase of 0.1 weight portion again: cellulase: the mixed enzyme stirring and dissolving of papain=5:2:3, enzymolysis 5h under the condition of vacuum-0.06MPa and temperature 50 C;
C) by sample microwave radiation exaraction 2h under the condition of temperature 90 DEG C;
D) sample is centrifugal, filter, filtrate, with after food-grade extraction into ethyl acetate, isolates aqueous phase with separatory funnel, and food-grade ethyl acetate is removed with Rotary Evaporators reduced pressure concentration by its organic phase at 50 ~ 60 DEG C, obtains matsutake and extracts oil.
Get matsutake and extract oil 2%, olive oil 70%, deep sea fish oil 25%, yak oil 3%, is first heated to 80 DEG C by olive oil, in the ratio determined, add deep sea fish oil and yak oil, mixing is filtered, then adds matsutake extraction oil, stirs with the speed of 90r/min, abundant mixing, filter, filling, obtained ready-mixed oil finished product.
Through aliphatic acid composition measuring, the ratio of SFA, MUFA, PUFA contained by this ready-mixed oil is that in 0.4:1.1:1, PUFA, ω-6 is 5.0:1 with the ratio of ω-3.
Embodiment 5
A) carry out ultra micro air-flow crushing by after matsutake freeze drying, cross 250 mesh sieves;
B) the matsutake powder of 1 weight portion is got, add the distilled water of 10 weight portions, add the chitinase of 0.08 weight portion again: cellulase: the mixed enzyme stirring and dissolving of papain=5:2:3, enzymolysis 5h under the condition of vacuum-0.06MPa and temperature 45 C;
C) by sample microwave radiation exaraction 4h under the condition of temperature 82 DEG C;
D) sample is centrifugal, filter, filtrate, with after food-grade extraction into ethyl acetate, isolates aqueous phase with separatory funnel, and food-grade ethyl acetate is removed with Rotary Evaporators reduced pressure concentration by its organic phase at 60 DEG C, obtains matsutake and extracts oil.
Get matsutake and extract oil 2%, olive oil 74%, deep sea fish oil 22%, yak oil 2%, is first heated to 65 DEG C by olive oil, in the ratio determined, add deep sea fish oil and yak oil, mixing is filtered, then adds matsutake extraction oil, stirs with the speed of 110r/min, abundant mixing, filter, filling, obtained ready-mixed oil finished product.
Through aliphatic acid composition measuring, the ratio of SFA, MUFA, PUFA contained by this ready-mixed oil is that in 0.5:1.4:1, PUFA, ω-6 is 4.8:1 with the ratio of ω-3.
Although the present invention with preferred embodiment openly as above; but it is also not used to limit the present invention, any person skilled in the art, without departing from the spirit and scope of the present invention; all can do various changes and modification, what therefore protection scope of the present invention should define with claims is as the criterion.
Claims (6)
1. a matsutake extracts oil, it is characterized in that, obtains according to the following steps:
A) carry out ultra micro air-flow crushing by after matsutake freeze drying, cross 100 ~ 300 mesh sieves;
B) the matsutake powder of 1 weight portion is got, add the distilled water of 10 ~ 20 weight portions, add the chitinase of 0.01 ~ 0.1 weight portion again: cellulase: the mixed enzyme stirring and dissolving of papain=5:2:3, enzymolysis 2 ~ 8h under the condition of vacuum-0.02 ~-0.08MPa and temperature 45 ~ 55 DEG C;
C) by sample microwave radiation exaraction 2 ~ 4h under the condition of temperature 80 ~ 95 DEG C;
D) sample is centrifugal, filter, after filtrate organic solvent extraction, isolate aqueous phase with separatory funnel, organic solvent is removed with Rotary Evaporators reduced pressure concentration by its organic phase at 50 ~ 60 DEG C, obtains matsutake and extracts oil.
2. matsutake according to claim 1 extracts oil, and it is characterized in that, described organic solvent is food-grade ethyl acetate.
3. a ready-mixed oil, extracts oil containing, for example matsutake according to claim 1.
4. ready-mixed oil according to claim 3, is characterized in that, also containing olive oil, deep sea fish oil and yak oil.
5. ready-mixed oil according to claim 4, is characterized in that, the mass ratio of each component is that matsutake extracts oil 1 ~ 2%, olive oil 70 ~ 87%, deep sea fish oil 10 ~ 25%, yak oil 2 ~ 3%.
6. the preparation method as ready-mixed oil in claim 5, prepared by following steps: olive oil is heated to 60 ~ 80 DEG C, add deep sea fish oil and yak oil, mixing is filtered, then adds matsutake extraction oil, stirs with the speed of 80 ~ 120r/min, abundant mixing, filter, filling, obtained ready-mixed oil finished product.
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Cited By (6)
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| CN105362792A (en) * | 2015-12-23 | 2016-03-02 | 焦福艳 | Nanometer Chinese herbal medicine care solution for pruritus vulvae and preparation method of care solution |
| CN106221916A (en) * | 2016-07-22 | 2016-12-14 | 深圳波顿香料有限公司 | The extracting method of a kind of Pericarpium Zanthoxyli fragrance ingredient and extract, application |
| CN107233334A (en) * | 2017-05-15 | 2017-10-10 | 新乡医学院 | A kind of pharmaceutical composition for adjusting immunity and its application |
| CN107260711A (en) * | 2017-05-15 | 2017-10-20 | 新乡医学院 | A kind of pharmaceutical composition for treating breast cancer and its application |
| CN107259495A (en) * | 2017-06-22 | 2017-10-20 | 龙牌食品股份有限公司 | A kind of capsicum fries special soy sauce of meat and preparation method thereof |
| CN108707507A (en) * | 2018-05-28 | 2018-10-26 | 云南能投野生菌农业发展有限公司 | A kind of truffle oil and preparation method thereof |
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| CN105362792A (en) * | 2015-12-23 | 2016-03-02 | 焦福艳 | Nanometer Chinese herbal medicine care solution for pruritus vulvae and preparation method of care solution |
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| CN107233334A (en) * | 2017-05-15 | 2017-10-10 | 新乡医学院 | A kind of pharmaceutical composition for adjusting immunity and its application |
| CN107260711A (en) * | 2017-05-15 | 2017-10-20 | 新乡医学院 | A kind of pharmaceutical composition for treating breast cancer and its application |
| CN107259495A (en) * | 2017-06-22 | 2017-10-20 | 龙牌食品股份有限公司 | A kind of capsicum fries special soy sauce of meat and preparation method thereof |
| CN108707507A (en) * | 2018-05-28 | 2018-10-26 | 云南能投野生菌农业发展有限公司 | A kind of truffle oil and preparation method thereof |
| CN108707507B (en) * | 2018-05-28 | 2020-11-24 | 云南能投野生菌农业发展有限公司 | Truffle oil and preparation method thereof |
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Application publication date: 20150429 |