CN104548205B - A kind of animal sources Nerve Scaffold and preparation method thereof - Google Patents
A kind of animal sources Nerve Scaffold and preparation method thereof Download PDFInfo
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- CN104548205B CN104548205B CN201410835135.XA CN201410835135A CN104548205B CN 104548205 B CN104548205 B CN 104548205B CN 201410835135 A CN201410835135 A CN 201410835135A CN 104548205 B CN104548205 B CN 104548205B
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- animal sources
- nerve
- coating materials
- support
- scaffold
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
- A61L27/04—Metals or alloys
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Medicinal Chemistry (AREA)
- Dermatology (AREA)
- General Health & Medical Sciences (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Veterinary Medicine (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Materials For Medical Uses (AREA)
- Prostheses (AREA)
Abstract
The invention provides a kind of animal sources Nerve Scaffold, include the degradable metal silk of support coating materials and support coating materials parcel, the main component of the support coating materials is animal sources blood vessel, lymphatic vessel, nerve channel or enteron aisle.The Nerve Scaffold is animal origin, solves the problem of autologous nerve is insufficient, also solves nerve autograft and necessarily cause new neurotrosis, causes to supply the impaired problem in area, CO2 laser weld is had far-reaching significance.
Description
Technical field
The present invention relates to CO2 laser weld technical field, in particular it relates to a kind of animal sources Nerve Scaffold and preparation method thereof.
Background technology
Because peripheral nerve injury caused by various traumatic origins or fracture cause the wounded to feel the decline with motor function
Or lose.Serious peripheral nerve injury often leads to patient's paralysis or permanent loss labour.Peripheral nerve injury is in China
The incidence of disease is higher, according to statistics, in traumatic patient, and four limbs injured nerves account for there are about in the 10% of wound sum, gunshot fracture
60% merging injured nerves.Therefore, need to find preferable peripheral nerve hinders treatment means.For peripheral nerve fracture class damage,
If fracture breach is larger, it must just be repaiied by can just make the peripheral nerve of fracture obtain regeneration breach progress bridge joint transfer operation
It is multiple.
At present, conventional transplant has artificial Nerve Scaffold and autotransplantation.In terms of artificial neuron support, due to non-degradable
Tube material may cause the risk of neurothlipsis and second operation, and the main of Recent study is intended to Biodegradable scaffold
The R and D of material.Currently, the U.S. has been approved by a variety of brands, e.g., NeuraGen, SaluBridge, Neurolac etc.
Nerve trachea be used for clinic.However, research of the China in terms of Nerve Scaffold more falls behind, do not develop with autonomous
The nerve trachea of intellectual property.
Autotransplantation is undoubtedly optimal neurologic defect restorative procedure, however, many experiments and clinical effectiveness are confirmed certainly
The effect of somatic nerves transplanting is better than man-made support.It is well known, however, that nerve autograft necessarily causes new neurotrosis,
Cause to be damaged for area's function, or even be partially formed scar, neuroma causes motion or sensory disturbance.Moreover, autologous nerve is originated
It is limited, especially for important thick neural Long nerve defect, it is often difficult to find suitable donor.So that autologous god
Development through transplanting is extremely limited, it is difficult to promote.Therefore, neurologic defect repair materials and the side for substituting nerve autograft are found
Method is particularly necessary.
The content of the invention
In order to overcome the deficiencies in the prior art, the invention provides a kind of animal sources Nerve Scaffold and preparation method thereof, god
It is animal origin through support, solves the problem of autologous nerve is insufficient, also solve nerve autograft and necessarily cause
New neurotrosis, causes to supply the impaired problem in area, CO2 laser weld is had far-reaching significance.
Technical scheme is as follows：A kind of animal sources Nerve Scaffold, including support coating materials and support coating materials parcel
Degradable metal silk, the main component of the support coating materials is animal sources blood vessel, lymphatic vessel, nerve channel or enteron aisle.
The support is sheet, strip or cylindric.
The main component of the degradable metal silk is magnesium, zinc, calcium, iron or its alloy.
The degradable metal silk is distributed along the Nerve Scaffold vertical and horizontal.
The preparation method of the animal sources Nerve Scaffold, comprises the following steps：
1) animal sources coating materials powder：Worn into after animal sources blood vessel, lymphatic vessel, nerve channel or enteron aisle freeze-drying, disinfection
Powder, obtains animal sources coating materials powder；
2) support is prepared：Animal sources coating materials powder is dissolved completely in dichloromethane, ultrasonic disperse is uniform, obtains slurry,
It is transferred in the mold cavity of masking mould, places horizontal and vertical degradable metal silk in mold cavity along rack forming direction, make
In degradable metal silk immersion slurry, solvent nature volatile dry vacuumizes 48h, low temperature drying, dry support coating materials parcel
The magnesium silk, obtains the animal sources Nerve Scaffold.
The raw materials such as animal sources blood vessel, lymphatic vessel, nerve channel or enteron aisle and human body have good biocompatibility, Neng Gouwei
Nerve growth provides morphologic support, and SEM shows that the internal structure of gained animal sources Nerve Scaffold is
It is cellular, creeping and growing and provide space for nerve cell；The degradable metal and its alloy can guide neural thin
Intracellular growth, so as to be conducive to the reparation of nerve cell.
Beneficial effects of the present invention are：Nerve Scaffold of the present invention is animal origin, solves autologous nerve supply
Not enough the problem of, also solve nerve autograft and necessarily cause new neurotrosis, cause to supply the impaired problem in area, it is right
CO2 laser weld has far-reaching significance.
Brief description of the drawings：
Fig. 1 is the structural representation of the artificial neuron support of the present invention rich in collagen.
Embodiment
The invention will be further described with preferred embodiment below in conjunction with the accompanying drawings, but embodiments of the present invention are not limited
In this.
The degradable metal that reference picture 1, a kind of animal sources Nerve Scaffold, including support coating materials 1 and support coating materials 1 are wrapped up
Silk 2.The degradable metal silk 2 is distributed along the vertical and horizontal of the Nerve Scaffold.
The support can be according to using needing to be prepared into variously-shaped, generally cylindrical cross section or sheet.
The main component of the support coating materials 1 is animal sources blood vessel, lymphatic vessel, nerve channel or enteron aisle.The degradable gold
The main component for belonging to silk 2 is magnesium, zinc, calcium, iron or its alloy.
Embodiment 1：The preparation method of the animal sources Nerve Scaffold, comprises the following steps：
1) animal sources coating materials powder：Animal sources blood vessel is freeze-dried, grinds after disinfection, obtains animal sources coating materials powder；
2) support is prepared：Animal sources coating materials powder is dissolved completely in dichloromethane, ultrasonic disperse is uniform, obtains slurry,
It is transferred in the mold cavity of masking mould, places horizontal and vertical magnesium-zinc alloy silk in mold cavity along rack forming direction, make magnesium
In saxonia metal wire immersion slurry, solvent nature volatile dry vacuumizes 48h, and low temperature drying, dry support coating materials parcel is described
Magnesium silk, obtains the animal sources Nerve Scaffold.
Embodiment 2：The preparation method of the animal sources Nerve Scaffold, comprises the following steps：
1) animal sources coating materials powder：Animal sources nerve channel is freeze-dried, grinds after disinfection, obtains animal sources coating materials
Powder；
2) support is prepared：Animal sources coating materials powder is dissolved completely in dichloromethane, ultrasonic disperse is uniform, obtains slurry,
It is transferred in the mold cavity of masking mould, places horizontal and vertical degradable metal silk in mold cavity along rack forming direction, make
In degradable metal silk immersion slurry, solvent nature volatile dry vacuumizes 48h, low temperature drying, dry support coating materials parcel
The magnesium silk, obtains the animal sources Nerve Scaffold.
Embodiment 3：The preparation method of the animal sources Nerve Scaffold, comprises the following steps：
1) animal sources coating materials powder：Animal sources enteron aisle is freeze-dried, grinds after disinfection, obtains animal sources coating materials powder；
2) support is prepared：Animal sources coating materials powder is dissolved completely in dichloromethane, ultrasonic disperse is uniform, obtains slurry,
It is transferred in the mold cavity of masking mould, places horizontal and vertical degradable metal silk in mold cavity along rack forming direction, make
In degradable metal silk immersion slurry, solvent nature volatile dry vacuumizes 48h, low temperature drying, dry support coating materials parcel
The magnesium silk, obtains the animal sources Nerve Scaffold.
Above content is to combine specific preferred embodiment further description made for the present invention, it is impossible to assert
The specific implementation of the present invention is confined to these explanations.For general technical staff of the technical field of the invention,
On the premise of not departing from present inventive concept, its framework form can be flexible and changeable, can be with subseries product.Simply make some
Simple deduction or replace, should all be considered as belonging to the scope of patent protection that the present invention is determined by the claims submitted.
Claims (3)
1. a kind of animal sources Nerve Scaffold, it is characterised in that the degradable metal wrapped up including support coating materials and support coating materials
Silk, the main component of the support coating materials is animal sources blood vessel, lymphatic vessel, nerve channel or enteron aisle, and SEM table
Bright, the internal structure of the animal sources Nerve Scaffold is cellular；
The degradable metal silk is distributed along the Nerve Scaffold vertical and horizontal.
2. animal sources Nerve Scaffold as claimed in claim 1, it is characterised in that the support is sheet, strip or cylinder
Shape.
3. the preparation method of animal sources Nerve Scaffold as claimed in claim 1 or 2, it is characterised in that comprise the following steps：
1) animal sources coating materials powder：Grinds after animal sources blood vessel, lymphatic vessel, nerve channel or enteron aisle freeze-drying, disinfection, are obtained
To animal sources coating materials powder；
2) support is prepared：Animal sources coating materials powder is dissolved completely in dichloromethane, ultrasonic disperse is uniform, obtains slurry, is transferred to
In the mold cavity of masking mould, horizontal and vertical degradable metal silk is placed in mold cavity along rack forming direction, makes to drop
Solve in wire immersion slurry, solvent nature volatile dry, vacuumize 48h, low temperature drying, dry support coating materials parcel is described
Degradable metal, obtains the animal sources Nerve Scaffold.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410835135.XA CN104548205B (en) | 2014-12-29 | 2014-12-29 | A kind of animal sources Nerve Scaffold and preparation method thereof |
| PCT/CN2015/082158 WO2016107107A1 (en) | 2014-12-29 | 2015-06-24 | Animal-derived nerve scaffold and manufacturing method therefor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410835135.XA CN104548205B (en) | 2014-12-29 | 2014-12-29 | A kind of animal sources Nerve Scaffold and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104548205A CN104548205A (en) | 2015-04-29 |
| CN104548205B true CN104548205B (en) | 2017-07-21 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410835135.XA Active CN104548205B (en) | 2014-12-29 | 2014-12-29 | A kind of animal sources Nerve Scaffold and preparation method thereof |
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| Country | Link |
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| CN (1) | CN104548205B (en) |
| WO (1) | WO2016107107A1 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104548205B (en) * | 2014-12-29 | 2017-07-21 | 东莞颠覆产品设计有限公司 | A kind of animal sources Nerve Scaffold and preparation method thereof |
| CN204394742U (en) * | 2014-12-29 | 2015-06-17 | 东莞颠覆产品设计有限公司 | A kind of real core Nerve Scaffold of built-in degradable metal silk |
| CN109513041B (en) * | 2018-12-29 | 2023-10-03 | 南通纺织丝绸产业技术研究院 | Nerve conduit with magnesium silk composite woven structure and preparation method thereof |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN200951213Y (en) * | 2005-12-20 | 2007-09-26 | 广州知光生物科技有限公司 | Biological type guide tube for nerve |
| US7942918B2 (en) * | 2005-12-29 | 2011-05-17 | Ethicon, Inc. | Device for treating carpal tunnel syndrome |
| CN101366975B (en) * | 2008-09-03 | 2011-07-20 | 陕西瑞盛生物科技有限公司 | Preparation method for cellfree intestinum tenue submucosa biological material |
| US20140099352A1 (en) * | 2012-10-08 | 2014-04-10 | Robert G. Matheny | Compositions, Structures and Methods for Neural Regeneration |
| CN104107096B (en) * | 2014-07-18 | 2016-09-07 | 上海交通大学 | Flexible degradable magnesium alloy nerve trachea and preparation method thereof |
| CN104548205B (en) * | 2014-12-29 | 2017-07-21 | 东莞颠覆产品设计有限公司 | A kind of animal sources Nerve Scaffold and preparation method thereof |
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2014
- 2014-12-29 CN CN201410835135.XA patent/CN104548205B/en active Active
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- 2015-06-24 WO PCT/CN2015/082158 patent/WO2016107107A1/en active Application Filing
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| Publication number | Publication date |
|---|---|
| WO2016107107A1 (en) | 2016-07-07 |
| CN104548205A (en) | 2015-04-29 |
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