WO2016107107A1 - Animal-derived nerve scaffold and manufacturing method therefor - Google Patents

Animal-derived nerve scaffold and manufacturing method therefor Download PDF

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WO2016107107A1
WO2016107107A1 PCT/CN2015/082158 CN2015082158W WO2016107107A1 WO 2016107107 A1 WO2016107107 A1 WO 2016107107A1 CN 2015082158 W CN2015082158 W CN 2015082158W WO 2016107107 A1 WO2016107107 A1 WO 2016107107A1
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stent
animal
nerve
film
derived
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French (fr)
Chinese (zh)
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李扬德
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东莞颠覆产品设计有限公司
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/02—Inorganic materials
    • A61L27/04—Metals or alloys
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials

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  • the invention relates to the technical field of nerve repair, and in particular to an animal-derived nerve stent and a preparation method thereof.
  • Peripheral nerve damage or breakage due to various trauma causes a decrease or loss of sensory and motor function. Severe peripheral nerve injury often leads to paralysis or permanent loss of labor. Peripheral nerve injury has a high incidence in China. According to statistics, in trauma patients, nerve injury of the extremities accounts for about 10% of the total number of trauma, and about 60% of the fractures of firearm injuries have neurological injuries. To this end, we need to find the ideal treatment for peripheral nerve injury. For peripheral nerve fractures, if the fracture gap is large, the fractured peripheral nerve must be regenerated and repaired by bridging the graft.
  • Autologous transplantation is undoubtedly the most ideal method for repairing nerve defects.
  • a large number of experimental and clinical results have confirmed that autologous nerve transplantation is superior to artificial stents.
  • autologous nerve transplantation inevitably causes new nerve damage, resulting in impaired function of the donor site, and even local formation of scars, neuroma, resulting in motor or sensory disturbances.
  • autologous nerve sources are limited, especially for long segments of important large nerves, and it is often difficult to find a suitable donor. Therefore, the development of autologous nerve transplantation is very limited and difficult to promote. Therefore, it is especially necessary to find nerve defect repair materials and methods to replace autologous nerve transplantation.
  • the present invention provides an animal-derived nerve stent and a preparation thereof
  • the nerve scaffold is of animal origin, solves the problem of insufficient supply of autologous nerves, and also solves the problem that autologous nerve transplantation inevitably causes new nerve damage, leading to impaired function of the donor area, and has far-reaching significance for nerve repair.
  • an animal-derived nerve stent comprising a stent film and a degradable metal wire wrapped by a stent film, the main component of which is an animal-derived blood vessel, a lymphatic vessel, a neural tube or an intestinal tract. .
  • the stent is in the form of a sheet, a strip or a cylinder.
  • the main component of the degradable metal wire is magnesium, zinc, calcium, iron or an alloy thereof.
  • the degradable wires are distributed along the longitudinal and transverse directions of the nerve stent.
  • the method for preparing the animal-derived nerve stent comprises the following steps:
  • Animal source film powder animal source blood vessels, lymphatic vessels, neural tubes or intestines are freeze-dried, sterilized and pulverized into powder, and animal source film powder is obtained;
  • the animal source film powder is completely dissolved in methylene chloride, and the ultrasonic dispersion is uniform, and the slurry is obtained, which is transferred into the mold cavity of the film forming mold, and the horizontal and vertical directions are placed in the mold cavity along the stent forming direction.
  • the metal wire is degraded, the degradable metal wire is immersed in the slurry, the solvent is naturally volatilized and dried, vacuumed for 48 hours, dried at a low temperature, and the dried stent film is wrapped with the magnesium wire to obtain the animal-derived nerve stent.
  • Raw materials such as blood vessels, lymphatic vessels, neural tubes or intestines have good biocompatibility with human body and can provide morphological support for nerve growth.
  • Scanning electron fiber mirrors show that the internal structure of the obtained animal-derived nerve scaffold is Honeycomb provides space for the nerve cells to crawl and grow; the degradable metals and their alloys can guide the growth of nerve cells, thereby facilitating the repair of nerve cells.
  • the invention has the beneficial effects that the nerve scaffold of the invention is of animal origin, solves the problem of insufficient supply of autologous nerve, and solves the problem that the autologous nerve transplantation inevitably causes new nerve damage, resulting in impaired function of the donor area, Nerve repair has far-reaching significance.
  • FIG. 1 is a schematic view showing the structure of a collagen-rich artificial nerve stent of the present invention.
  • an animal-derived nerve stent comprises a stent film 1 and a degradable metal wire 2 wrapped by a stent film 1.
  • the degradable metal wire 2 is distributed along the longitudinal and transverse directions of the nerve stent.
  • the stent can be prepared into various shapes according to the needs of use, and is generally cylindrical or sheet-shaped.
  • the main component of the scaffold film 1 is an animal-derived blood vessel, a lymphatic vessel, a neural tube or an intestinal tract.
  • the main component of the degradable metal wire 2 is magnesium, zinc, calcium, iron or an alloy thereof.
  • Example 1 A method for preparing the animal-derived nerve stent, comprising the following steps:
  • Animal source film powder animal source blood vessels are freeze-dried, sterilized and pulverized into powder, and animal source film powder is obtained;
  • the animal source film powder is completely dissolved in methylene chloride, the ultrasonic dispersion is uniform, the slurry is obtained, and transferred into the mold cavity of the film forming mold, and the transverse and longitudinal magnesium are placed in the mold cavity along the stent forming direction.
  • Zinc alloy wire, the magnesium-zinc alloy wire is immersed in the slurry, the solvent is naturally volatilized and dried, vacuumed for 48 hours, dried at a low temperature, and the dried stent film is wrapped with the magnesium wire to obtain the animal-derived nerve stent.
  • Example 2 A method for preparing the animal-derived nerve stent, comprising the following steps:
  • Animal source film powder the animal source neural tube is freeze-dried, sterilized and pulverized into powder, and the animal source film powder is obtained;
  • the animal source film powder is completely dissolved in methylene chloride, and the ultrasonic dispersion is uniform, and the slurry is obtained, which is transferred into the mold cavity of the film forming mold, and the horizontal and vertical directions are placed in the mold cavity along the stent forming direction.
  • the metal wire is degraded, the degradable metal wire is immersed in the slurry, the solvent is naturally volatilized and dried, vacuumed for 48 hours, dried at a low temperature, and the dried stent film is wrapped with the magnesium wire to obtain the animal-derived nerve stent.
  • Example 3 A method for preparing the animal-derived nerve stent, comprising the following steps:
  • Animal source film powder the animal source is lyophilized and dried, sterilized and pulverized into powder, and the animal source film powder is obtained;
  • the animal source film powder is completely dissolved in methylene chloride, and the ultrasonic dispersion is uniform, and the slurry is obtained, which is transferred into the mold cavity of the film forming mold, and the horizontal and vertical directions are placed in the mold cavity along the stent forming direction.
  • the metal wire is degraded, the degradable metal wire is immersed in the slurry, the solvent is naturally volatilized and dried, vacuumed for 48 hours, dried at a low temperature, and the dried stent film is wrapped with the magnesium wire to obtain the animal-derived nerve stent.

Abstract

An animal-derived nerve scaffold and a manufacturing method therefor. The animal-derived nerve scaffold comprises a scaffold membrane (1) and a degradable metal wire (2) wrapped by the scaffold membrane (1). The primary constituents of the scaffold membrane (1) are animal-derived blood vessels, lymphatic vessels, neural tubes or intestines. The manufacturing method comprises the steps of preparing an animal-derived membrane powder and wrapping the degradable metal wire (2) with the scaffold membrane (1).

Description

一种动物源神经支架及其制备方法Animal source nerve scaffold and preparation method thereof 技术领域Technical field
本发明涉及神经修复技术领域，具体地，涉及一种动物源神经支架及其制备方法。The invention relates to the technical field of nerve repair, and in particular to an animal-derived nerve stent and a preparation method thereof.
背景技术Background technique
由于各种外伤原因导致的周围神经损伤或断裂造成伤者感觉和运动功能的下降或丧失。严重的外周神经损伤往往导致患者瘫痪或永久丧失劳动力。外周神经损伤在我国发病率较高，据统计，在外伤患者中，四肢神经伤约占外伤总数的10％，火器伤骨折中约有60％的合并神经伤。为此，需寻找理想的外周神经伤治疗手段。对于外周神经断裂类损伤，若断裂缺口较大，就须借助对缺口进行桥接移植手术才能使断裂的周围神经得到再生修复。Peripheral nerve damage or breakage due to various trauma causes a decrease or loss of sensory and motor function. Severe peripheral nerve injury often leads to paralysis or permanent loss of labor. Peripheral nerve injury has a high incidence in China. According to statistics, in trauma patients, nerve injury of the extremities accounts for about 10% of the total number of trauma, and about 60% of the fractures of firearm injuries have neurological injuries. To this end, we need to find the ideal treatment for peripheral nerve injury. For peripheral nerve fractures, if the fracture gap is large, the fractured peripheral nerve must be regenerated and repaired by bridging the graft.
目前，常用的移植体有人工神经支架和自体移植。人工神经支架方面，由于非降解导管材料可能导致神经压迫和二次手术的风险，近年来研究的主要趋向于生物可降解支架材料的开发和研究。当前，美国已经批准多种品牌，如，NeuraGen、SaluBridge，Neurolac等的神经导管用于临床。然而，我国在神经支架方面的研究较为落后，并没有开发出具有自主知识产权的神经导管。At present, commonly used implants include artificial nerve stents and autografts. In terms of artificial nerve stents, the development of non-degradable catheter materials may lead to nerve compression and the risk of secondary surgery. In recent years, research has mainly led to the development and research of biodegradable stent materials. Currently, the United States has approved a variety of brands, such as NeuraGen, SaluBridge, Neurolac and other nerve catheters for clinical use. However, China's research on nerve scaffolds is relatively backward, and no neural catheters with independent intellectual property rights have been developed.
自体移植无疑是最理想的神经缺损修复方法，然而，大量实验和临床结果证实自体神经移植的效果优于人工支架。然而，众所周知，自体神经移植必然造成新的神经损伤，导致供区功能受损，甚至局部形成瘢痕，神经瘤，造成运动或感觉障碍。而且，自体神经来源有限，特别是对于重要的粗大神经的长段缺损，常常难以找到合适的供体。从而使得自体神经移植的发展十分有限，难以推广。因此，寻找替代自体神经移植的神经缺损修复材料和方法尤为必要。Autologous transplantation is undoubtedly the most ideal method for repairing nerve defects. However, a large number of experimental and clinical results have confirmed that autologous nerve transplantation is superior to artificial stents. However, it is well known that autologous nerve transplantation inevitably causes new nerve damage, resulting in impaired function of the donor site, and even local formation of scars, neuroma, resulting in motor or sensory disturbances. Moreover, autologous nerve sources are limited, especially for long segments of important large nerves, and it is often difficult to find a suitable donor. Therefore, the development of autologous nerve transplantation is very limited and difficult to promote. Therefore, it is especially necessary to find nerve defect repair materials and methods to replace autologous nerve transplantation.
发明内容Summary of the invention
为了克服现有技术的不足，本发明提供了一种动物源神经支架及其制备方 法，神经支架为动物来源的，解决了自体神经供应不足的问题，也解决了自体神经移植必然造成新的神经损伤，导致供区功能受损的问题，对神经修复具有深远的意义。In order to overcome the deficiencies of the prior art, the present invention provides an animal-derived nerve stent and a preparation thereof The method, the nerve scaffold is of animal origin, solves the problem of insufficient supply of autologous nerves, and also solves the problem that autologous nerve transplantation inevitably causes new nerve damage, leading to impaired function of the donor area, and has far-reaching significance for nerve repair.
本发明的技术方案如下：一种动物源神经支架，包括支架膜料以及支架膜料包裹的可降解金属丝，所述支架膜料的主要成分为动物源血管、淋巴管、神经管或肠道。The technical solution of the present invention is as follows: an animal-derived nerve stent comprising a stent film and a degradable metal wire wrapped by a stent film, the main component of which is an animal-derived blood vessel, a lymphatic vessel, a neural tube or an intestinal tract. .
所述支架为片状，长条状或圆柱状。The stent is in the form of a sheet, a strip or a cylinder.
所述可降解金属丝的主要成分为镁，锌，钙，铁或其合金。The main component of the degradable metal wire is magnesium, zinc, calcium, iron or an alloy thereof.
所述可降解金属丝沿所述神经支架纵向和横向分布。The degradable wires are distributed along the longitudinal and transverse directions of the nerve stent.
所述动物源神经支架的制备方法，包括如下步骤：The method for preparing the animal-derived nerve stent comprises the following steps:
1)动物源膜料粉：动物源血管、淋巴管、神经管或肠道冷冻干燥，消毒杀菌后磨成粉，得到动物源膜料粉；1) Animal source film powder: animal source blood vessels, lymphatic vessels, neural tubes or intestines are freeze-dried, sterilized and pulverized into powder, and animal source film powder is obtained;
2)制备支架：将动物源膜料粉完全溶解在二氯甲烷中，超声分散均匀，得到浆料，转入制膜模具的模具腔中，模具腔中沿支架成型方向放置横向和纵向的可降解金属丝，使可降解金属丝浸入浆料中，溶剂自然挥发干燥，抽真空48h，低温干燥，干燥的支架膜料包裹所述镁丝，得到所述动物源神经支架。2) Preparation of the stent: the animal source film powder is completely dissolved in methylene chloride, and the ultrasonic dispersion is uniform, and the slurry is obtained, which is transferred into the mold cavity of the film forming mold, and the horizontal and vertical directions are placed in the mold cavity along the stent forming direction. The metal wire is degraded, the degradable metal wire is immersed in the slurry, the solvent is naturally volatilized and dried, vacuumed for 48 hours, dried at a low temperature, and the dried stent film is wrapped with the magnesium wire to obtain the animal-derived nerve stent.
动物源血管、淋巴管、神经管或肠道等原料与人体具有良好的生物相容性，能够为神经生长提供形态学上的支撑，扫描电子纤维镜表明，所得动物源神经支架的内部结构为蜂窝状，为神经细胞的爬行和生长提供了空间；所述可降解金属及其合金可以引导神经细胞生长，从而有利于神经细胞的修复。Raw materials such as blood vessels, lymphatic vessels, neural tubes or intestines have good biocompatibility with human body and can provide morphological support for nerve growth. Scanning electron fiber mirrors show that the internal structure of the obtained animal-derived nerve scaffold is Honeycomb provides space for the nerve cells to crawl and grow; the degradable metals and their alloys can guide the growth of nerve cells, thereby facilitating the repair of nerve cells.
本发明的有益效果为：本发明所述神经支架为动物来源的，解决了自体神经供应不足的问题，也解决了自体神经移植必然造成新的神经损伤，导致供区功能受损的问题，对神经修复具有深远的意义。 The invention has the beneficial effects that the nerve scaffold of the invention is of animal origin, solves the problem of insufficient supply of autologous nerve, and solves the problem that the autologous nerve transplantation inevitably causes new nerve damage, resulting in impaired function of the donor area, Nerve repair has far-reaching significance.
附图说明：BRIEF DESCRIPTION OF THE DRAWINGS:
图1为本发明所述富含胶原蛋白的人工神经支架的结构示意图。1 is a schematic view showing the structure of a collagen-rich artificial nerve stent of the present invention.
具体实施方式detailed description
下面结合附图和优选实施例对本发明作进一步的描述，但本发明的实施方式不限于此。The present invention will be further described below in conjunction with the drawings and preferred embodiments, but the embodiments of the present invention are not limited thereto.
参照图1，一种动物源神经支架，包括支架膜料1以及支架膜料1包裹的可降解金属丝2。所述可降解金属丝2沿所述神经支架的纵向和横向分布。Referring to Fig. 1, an animal-derived nerve stent comprises a stent film 1 and a degradable metal wire 2 wrapped by a stent film 1. The degradable metal wire 2 is distributed along the longitudinal and transverse directions of the nerve stent.
所述支架可以根据使用需要制备成各种形状，一般为圆筒形，也可以是片状。The stent can be prepared into various shapes according to the needs of use, and is generally cylindrical or sheet-shaped.
所述支架膜料1的主要成分为动物源血管、淋巴管、神经管或肠道。所述可降解金属丝2的主要成分为镁，锌，钙，铁或其合金。The main component of the scaffold film 1 is an animal-derived blood vessel, a lymphatic vessel, a neural tube or an intestinal tract. The main component of the degradable metal wire 2 is magnesium, zinc, calcium, iron or an alloy thereof.
实施例1：所述动物源神经支架的制备方法，包括如下步骤：Example 1: A method for preparing the animal-derived nerve stent, comprising the following steps:
1)动物源膜料粉：动物源血管冷冻干燥，消毒杀菌后磨成粉，得到动物源膜料粉；1) Animal source film powder: animal source blood vessels are freeze-dried, sterilized and pulverized into powder, and animal source film powder is obtained;
2)制备支架：将动物源膜料粉完全溶解在二氯甲烷中，超声分散均匀，得到浆料，转入制膜模具的模具腔中，模具腔中沿支架成型方向放置横向和纵向的镁锌合金丝，使镁锌合金丝浸入浆料中，溶剂自然挥发干燥，抽真空48h，低温干燥，干燥的支架膜料包裹所述镁丝，得到所述动物源神经支架。2) Preparation of the stent: the animal source film powder is completely dissolved in methylene chloride, the ultrasonic dispersion is uniform, the slurry is obtained, and transferred into the mold cavity of the film forming mold, and the transverse and longitudinal magnesium are placed in the mold cavity along the stent forming direction. Zinc alloy wire, the magnesium-zinc alloy wire is immersed in the slurry, the solvent is naturally volatilized and dried, vacuumed for 48 hours, dried at a low temperature, and the dried stent film is wrapped with the magnesium wire to obtain the animal-derived nerve stent.
实施例2：所述动物源神经支架的制备方法，包括如下步骤： Example 2: A method for preparing the animal-derived nerve stent, comprising the following steps:
1)动物源膜料粉：动物源神经管冷冻干燥，消毒杀菌后磨成粉，得到动物源膜料粉；1) Animal source film powder: the animal source neural tube is freeze-dried, sterilized and pulverized into powder, and the animal source film powder is obtained;
2)制备支架：将动物源膜料粉完全溶解在二氯甲烷中，超声分散均匀，得到浆料，转入制膜模具的模具腔中，模具腔中沿支架成型方向放置横向和纵向的可降解金属丝，使可降解金属丝浸入浆料中，溶剂自然挥发干燥，抽真空48h，低温干燥，干燥的支架膜料包裹所述镁丝，得到所述动物源神经支架。2) Preparation of the stent: the animal source film powder is completely dissolved in methylene chloride, and the ultrasonic dispersion is uniform, and the slurry is obtained, which is transferred into the mold cavity of the film forming mold, and the horizontal and vertical directions are placed in the mold cavity along the stent forming direction. The metal wire is degraded, the degradable metal wire is immersed in the slurry, the solvent is naturally volatilized and dried, vacuumed for 48 hours, dried at a low temperature, and the dried stent film is wrapped with the magnesium wire to obtain the animal-derived nerve stent.
实施例3：所述动物源神经支架的制备方法，包括如下步骤：Example 3: A method for preparing the animal-derived nerve stent, comprising the following steps:
1)动物源膜料粉：动物源肠道冷冻干燥，消毒杀菌后磨成粉，得到动物源膜料粉；1) Animal source film powder: the animal source is lyophilized and dried, sterilized and pulverized into powder, and the animal source film powder is obtained;
2)制备支架：将动物源膜料粉完全溶解在二氯甲烷中，超声分散均匀，得到浆料，转入制膜模具的模具腔中，模具腔中沿支架成型方向放置横向和纵向的可降解金属丝，使可降解金属丝浸入浆料中，溶剂自然挥发干燥，抽真空48h，低温干燥，干燥的支架膜料包裹所述镁丝，得到所述动物源神经支架。2) Preparation of the stent: the animal source film powder is completely dissolved in methylene chloride, and the ultrasonic dispersion is uniform, and the slurry is obtained, which is transferred into the mold cavity of the film forming mold, and the horizontal and vertical directions are placed in the mold cavity along the stent forming direction. The metal wire is degraded, the degradable metal wire is immersed in the slurry, the solvent is naturally volatilized and dried, vacuumed for 48 hours, dried at a low temperature, and the dried stent film is wrapped with the magnesium wire to obtain the animal-derived nerve stent.
以上内容是结合具体的优选实施方式对本发明所作的进一步详细说明，不能认定本发明的具体实施只局限于这些说明。对于本发明所属技术领域的普通技术人员来说，在不脱离本发明构思的前提下，其架构形式能够灵活多变，可以派生系列产品。只是做出若干简单推演或替换，都应当视为属于本发明由所提交的权利要求书确定的专利保护范围。 The above is a further detailed description of the present invention in connection with the specific preferred embodiments, and the specific embodiments of the present invention are not limited to the description. For those skilled in the art to which the present invention pertains, the architectural form can be flexible and can be derived from a series of products without departing from the inventive concept. It is to be understood that the invention is intended to be limited to the scope of the invention as defined by the appended claims.

Claims (5)

  1. 一种动物源神经支架，其特征在于，包括支架膜料以及支架膜料包裹的可降解金属丝，所述支架膜料的主要成分为动物源血管、淋巴管、神经管或肠道。An animal-derived nerve stent is characterized in that it comprises a stent film and a degradable metal wire wrapped by a stent film, and the main component of the stent film is an animal-derived blood vessel, a lymphatic vessel, a neural tube or an intestinal tract.
  2. 如权利要求1所述的动物源神经支架，其特征在于，所述支架为片状，长条状或圆柱状。The animal-derived nerve stent according to claim 1, wherein the stent is in the form of a sheet, an elongated strip or a cylinder.
  3. 如权利要求1所述的动物源神经支架，其特征在于，所述可降解金属丝的主要成分为镁，锌，钙，铁或其合金。The animal-derived nerve stent according to claim 1, wherein the main component of the degradable metal wire is magnesium, zinc, calcium, iron or an alloy thereof.
  4. 如权利要求1所述的动物源神经支架，其特征在于，所述可降解金属丝沿所述神经支架纵向和横向分布。The animal-derived nerve stent of claim 1 wherein said degradable metal wire is distributed longitudinally and laterally of said nerve stent.
  5. 如权利要求1-4任一项所述动物源神经支架的制备方法，其特征在于，包括如下步骤：The method for preparing an animal-derived nerve stent according to any one of claims 1 to 4, comprising the steps of:
    1)动物源膜料粉：动物源血管、淋巴管、神经管或肠道冷冻干燥，消毒杀菌后磨成粉，得到动物源膜料粉；1) Animal source film powder: animal source blood vessels, lymphatic vessels, neural tubes or intestines are freeze-dried, sterilized and pulverized into powder, and animal source film powder is obtained;
    2)制备支架：将动物源膜料粉完全溶解在二氯甲烷中，超声分散均匀，得到浆料，转入制膜模具的模具腔中，模具腔中沿支架成型方向放置横向和纵向的可降解金属丝，使可降解金属丝浸入浆料中，溶剂自然挥发干燥，抽真空48h，低温干燥，干燥的支架膜料包裹所述镁丝，得到所述动物源神经支架。 2) Preparation of the stent: the animal source film powder is completely dissolved in methylene chloride, and the ultrasonic dispersion is uniform, and the slurry is obtained, which is transferred into the mold cavity of the film forming mold, and the horizontal and vertical directions are placed in the mold cavity along the stent forming direction. The metal wire is degraded, the degradable metal wire is immersed in the slurry, the solvent is naturally volatilized and dried, vacuumed for 48 hours, dried at a low temperature, and the dried stent film is wrapped with the magnesium wire to obtain the animal-derived nerve stent.
PCT/CN2015/082158 2014-12-29 2015-06-24 Animal-derived nerve scaffold and manufacturing method therefor WO2016107107A1 (en)

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CN104548205B (en) * 2014-12-29 2017-07-21 东莞颠覆产品设计有限公司 A kind of animal sources Nerve Scaffold and preparation method thereof
CN109513041B (en) * 2018-12-29 2023-10-03 南通纺织丝绸产业技术研究院 Nerve conduit with magnesium silk composite woven structure and preparation method thereof

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