CN104548205A - Animal-derived nerve scaffold and preparation method thereof - Google Patents
Animal-derived nerve scaffold and preparation method thereof Download PDFInfo
- Publication number
- CN104548205A CN104548205A CN201410835135.XA CN201410835135A CN104548205A CN 104548205 A CN104548205 A CN 104548205A CN 201410835135 A CN201410835135 A CN 201410835135A CN 104548205 A CN104548205 A CN 104548205A
- Authority
- CN
- China
- Prior art keywords
- animal sources
- nerve
- coating materials
- scaffold
- nerve scaffold
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
- A61L27/04—Metals or alloys
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
Abstract
The invention provides an animal-derived nerve scaffold which comprises a scaffold membrane material and degradable metal wires wrapped in the scaffold membrane material, wherein main components of the scaffold membrane material are animal-derived blood vessels, lymphatic vessels, neural tubes or intestinal tracts. The animal-derived nerve scaffold is derived from animals, so that the problem of short supply of autologous nerves is solved, the problem of donor site function damage caused by new nerve injury inevitably caused by autologous neural transplantation is also solved, and the nerve scaffold has far-reaching significance for nerve repair.
Description
Technical field
The present invention relates to CO2 laser weld technical field, particularly, relate to a kind of animal sources Nerve Scaffold and preparation method thereof.
Background technology
The peripheral nerve injury caused due to various traumatic origin or fracture cause the wounded to feel and the decline of motor function or forfeiture.Serious peripheral nerve injury often causes patient to paralyse or permanent loss labour force.Peripheral nerve injury is higher at China's sickness rate, and according to statistics, in traumatic patient, extremity injured nerves accounts for 10% of wound sum, about has the merging injured nerves of 60% in gunshot fracture.For this reason, desirable peripheral nervous need be found and hinder treatment means.For the damage of peripheral nervous fracture class, if fracture breach is comparatively large, just the peripheral nerve of fracture just can must be made to obtain Regeneration and Repair by carrying out breach bridge joint transplant operation.
At present, conventional transplant has artificial neuron support and autotransplantation.Artificial neuron support aspect, because non-degradable tube material may cause the risk of nerve compression and second operation, the R and D mainly trending towards Biodegradable scaffold material of Recent study.Current, the U.S. has ratified multiple brand, and e.g., NeuraGen, SaluBridge, the nerve trachea of Neurolac etc. is used for clinical.But the research of China in Nerve Scaffold comparatively backwardness, does not develop the nerve trachea with independent intellectual property right.
Autotransplantation is optimal neurologic defect restorative procedure beyond doubt, but great many of experiments and clinical effectiveness confirm that the effect of nerve autograft is better than man-made support.But as everyone knows, nerve autograft must cause new nerve injury, cause for district's function impaired, be even partially formed cicatrix, neuroma, cause motion or sensory disturbance.And autologous nerve limited source, particularly for the Long nerve defect of important thick nerve, is usually difficult to find suitable donor.Thus make the development of nerve autograft very limited, be difficult to promote.Therefore, neurologic defect repair materials and the method for finding alternative nerve autograft are particularly necessary.
Summary of the invention
In order to overcome the deficiencies in the prior art, the invention provides a kind of animal sources Nerve Scaffold and preparation method thereof, Nerve Scaffold is animal origin, solve the problem that autologous nerve is under-supply, also solve nerve autograft and must cause new nerve injury, cause, for the impaired problem of district's function, to CO2 laser weld, there is profound significance.
Technical scheme of the present invention is as follows: a kind of animal sources Nerve Scaffold, and comprise the degradable metal silk of support coating materials and support coating materials parcel, the main component of described support coating materials is animal sources blood vessel, lymphatic vessel, neurocele or intestinal.
Described support is lamellar, strip or cylindric.
The main component of described degradable metal silk is magnesium, zinc, calcium, ferrum or its alloy.
Described degradable metal silk distributes along described Nerve Scaffold vertical and horizontal.
The preparation method of described animal sources Nerve Scaffold, comprises the steps:
1) animal sources coating materials powder: animal sources blood vessel, lymphatic vessel, neurocele or intestinal lyophilization, grinds after disinfection, obtains animal sources coating materials powder;
2) support is prepared: be dissolved completely in dichloromethane by animal sources coating materials powder, ultrasonic disperse is even, obtain slurry, proceed in the mold cavity of masking mould, in mold cavity, place horizontal and vertical degradable metal silk along rack forming direction, degradable metal silk is made to immerse in slurry, solvent nature volatile dry, evacuation 48h, cold drying, dry support coating materials wraps up described magnesium silk, obtains described animal sources Nerve Scaffold.
The raw materials such as animal sources blood vessel, lymphatic vessel, neurocele or intestinal and human body have good biocompatibility, morphologic support can be provided for nerve growth, scanning electron fibrescope shows, the internal structure of gained animal sources Nerve Scaffold is cellular, for neurocyte creep and growth provides space; Described degradable metal and alloy thereof can guide nerve growth, thus are conducive to the reparation of neurocyte.
Beneficial effect of the present invention is: Nerve Scaffold of the present invention is animal origin, solve the problem that autologous nerve is under-supply, also solve nerve autograft and must cause new nerve injury, cause, for the impaired problem of district's function, to CO2 laser weld, there is profound significance.
Accompanying drawing illustrates:
Fig. 1 is the structural representation being rich in the artificial neuron support of collagen protein of the present invention.
Detailed description of the invention
Below in conjunction with accompanying drawing and preferred embodiment, the invention will be further described, but embodiments of the present invention are not limited thereto.
With reference to Fig. 1, a kind of animal sources Nerve Scaffold, comprises the degradable metal silk 2 of support coating materials 1 and support coating materials 1 parcel.Described degradable metal silk 2 distributes along the vertical and horizontal of described Nerve Scaffold.
Described support can need to be prepared into various shape according to use, is generally cylindrical shape, also can be lamellar.
The main component of described support coating materials 1 is animal sources blood vessel, lymphatic vessel, neurocele or intestinal.The main component of described degradable metal silk 2 is magnesium, zinc, calcium, ferrum or its alloy.
Embodiment 1: the preparation method of described animal sources Nerve Scaffold, comprises the steps:
1) animal sources coating materials powder: animal sources blood vessel lyophilization, grinds after disinfection, obtains animal sources coating materials powder;
2) support is prepared: be dissolved completely in dichloromethane by animal sources coating materials powder, ultrasonic disperse is even, obtain slurry, proceed in the mold cavity of masking mould, in mold cavity, place horizontal and vertical magnesium-zinc alloy silk along rack forming direction, magnesium-zinc alloy silk is made to immerse in slurry, solvent nature volatile dry, evacuation 48h, cold drying, dry support coating materials wraps up described magnesium silk, obtains described animal sources Nerve Scaffold.
Embodiment 2: the preparation method of described animal sources Nerve Scaffold, comprises the steps:
1) animal sources coating materials powder: animal sources neurocele lyophilization, grinds after disinfection, obtains animal sources coating materials powder;
2) support is prepared: be dissolved completely in dichloromethane by animal sources coating materials powder, ultrasonic disperse is even, obtain slurry, proceed in the mold cavity of masking mould, in mold cavity, place horizontal and vertical degradable metal silk along rack forming direction, degradable metal silk is made to immerse in slurry, solvent nature volatile dry, evacuation 48h, cold drying, dry support coating materials wraps up described magnesium silk, obtains described animal sources Nerve Scaffold.
Embodiment 3: the preparation method of described animal sources Nerve Scaffold, comprises the steps:
1) animal sources coating materials powder: animal sources intestinal lyophilization, grinds after disinfection, obtains animal sources coating materials powder;
2) support is prepared: be dissolved completely in dichloromethane by animal sources coating materials powder, ultrasonic disperse is even, obtain slurry, proceed in the mold cavity of masking mould, in mold cavity, place horizontal and vertical degradable metal silk along rack forming direction, degradable metal silk is made to immerse in slurry, solvent nature volatile dry, evacuation 48h, cold drying, dry support coating materials wraps up described magnesium silk, obtains described animal sources Nerve Scaffold.
Above content is in conjunction with concrete preferred implementation further description made for the present invention, can not assert that specific embodiment of the invention is confined to these explanations.For general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, its framework form can be flexible and changeable, can subseries product.Just make some simple deduction or replace, all should be considered as belonging to the scope of patent protection that the present invention is determined by submitted to claims.
Claims (5)
1. an animal sources Nerve Scaffold, is characterized in that, comprise the degradable metal silk of support coating materials and support coating materials parcel, the main component of described support coating materials is animal sources blood vessel, lymphatic vessel, neurocele or intestinal.
2. animal sources Nerve Scaffold as claimed in claim 1, it is characterized in that, described support is lamellar, strip or cylindric.
3. animal sources Nerve Scaffold as claimed in claim 1, it is characterized in that, the main component of described degradable metal silk is magnesium, zinc, calcium, ferrum or its alloy.
4. animal sources Nerve Scaffold as claimed in claim 1, it is characterized in that, described degradable metal silk distributes along described Nerve Scaffold vertical and horizontal.
5. the preparation method of animal sources Nerve Scaffold as described in any one of claim 1-4, is characterized in that, comprise the steps:
1) animal sources coating materials powder: animal sources blood vessel, lymphatic vessel, neurocele or intestinal lyophilization, grinds after disinfection, obtains animal sources coating materials powder;
2) support is prepared: be dissolved completely in dichloromethane by animal sources coating materials powder, ultrasonic disperse is even, obtain slurry, proceed in the mold cavity of masking mould, in mold cavity, place horizontal and vertical degradable metal silk along rack forming direction, degradable metal silk is made to immerse in slurry, solvent nature volatile dry, evacuation 48h, cold drying, dry support coating materials wraps up described magnesium silk, obtains described animal sources Nerve Scaffold.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410835135.XA CN104548205B (en) | 2014-12-29 | 2014-12-29 | A kind of animal sources Nerve Scaffold and preparation method thereof |
| PCT/CN2015/082158 WO2016107107A1 (en) | 2014-12-29 | 2015-06-24 | Animal-derived nerve scaffold and manufacturing method therefor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410835135.XA CN104548205B (en) | 2014-12-29 | 2014-12-29 | A kind of animal sources Nerve Scaffold and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104548205A true CN104548205A (en) | 2015-04-29 |
| CN104548205B CN104548205B (en) | 2017-07-21 |
Family
ID=53065967
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410835135.XA Active CN104548205B (en) | 2014-12-29 | 2014-12-29 | A kind of animal sources Nerve Scaffold and preparation method thereof |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN104548205B (en) |
| WO (1) | WO2016107107A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016107107A1 (en) * | 2014-12-29 | 2016-07-07 | 东莞颠覆产品设计有限公司 | Animal-derived nerve scaffold and manufacturing method therefor |
| WO2016107106A1 (en) * | 2014-12-29 | 2016-07-07 | 东莞颠覆产品设计有限公司 | Solid-core nerve scaffold having built-in degradable metal wire |
| WO2020133668A1 (en) * | 2018-12-29 | 2020-07-02 | 南通纺织丝绸产业技术研究院 | Nerve conduit of magnesium filament and silk compositely woven structure and preparation method for nerve conduit |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN200951213Y (en) * | 2005-12-20 | 2007-09-26 | 广州知光生物科技有限公司 | Biological type guide tube for nerve |
| CN101366975A (en) * | 2008-09-03 | 2009-02-18 | 陕西瑞盛生物科技有限公司 | Preparation method for cellfree intestinum tenue submucosa biological material |
| US7942918B2 (en) * | 2005-12-29 | 2011-05-17 | Ethicon, Inc. | Device for treating carpal tunnel syndrome |
| WO2014058586A1 (en) * | 2012-10-08 | 2014-04-17 | Cormatrix Cardiovascular, Inc. | Compositions, structures and methods for neural regeneration |
| CN104107096A (en) * | 2014-07-18 | 2014-10-22 | 上海交通大学 | Bendable fully-degrading magnesium alloy nerve conduit and preparing method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104548205B (en) * | 2014-12-29 | 2017-07-21 | 东莞颠覆产品设计有限公司 | A kind of animal sources Nerve Scaffold and preparation method thereof |
-
2014
- 2014-12-29 CN CN201410835135.XA patent/CN104548205B/en active Active
-
2015
- 2015-06-24 WO PCT/CN2015/082158 patent/WO2016107107A1/en active Application Filing
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN200951213Y (en) * | 2005-12-20 | 2007-09-26 | 广州知光生物科技有限公司 | Biological type guide tube for nerve |
| US7942918B2 (en) * | 2005-12-29 | 2011-05-17 | Ethicon, Inc. | Device for treating carpal tunnel syndrome |
| CN101366975A (en) * | 2008-09-03 | 2009-02-18 | 陕西瑞盛生物科技有限公司 | Preparation method for cellfree intestinum tenue submucosa biological material |
| WO2014058586A1 (en) * | 2012-10-08 | 2014-04-17 | Cormatrix Cardiovascular, Inc. | Compositions, structures and methods for neural regeneration |
| CN104107096A (en) * | 2014-07-18 | 2014-10-22 | 上海交通大学 | Bendable fully-degrading magnesium alloy nerve conduit and preparing method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016107107A1 (en) * | 2014-12-29 | 2016-07-07 | 东莞颠覆产品设计有限公司 | Animal-derived nerve scaffold and manufacturing method therefor |
| WO2016107106A1 (en) * | 2014-12-29 | 2016-07-07 | 东莞颠覆产品设计有限公司 | Solid-core nerve scaffold having built-in degradable metal wire |
| WO2020133668A1 (en) * | 2018-12-29 | 2020-07-02 | 南通纺织丝绸产业技术研究院 | Nerve conduit of magnesium filament and silk compositely woven structure and preparation method for nerve conduit |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2016107107A1 (en) | 2016-07-07 |
| CN104548205B (en) | 2017-07-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Han et al. | Therapeutic strategies for peripheral nerve injury: decellularized nerve conduits and Schwann cell transplantation | |
| CN102580153B (en) | Method for preparing allograft acellular dermal matrixes | |
| CN104307045B (en) | A kind of preparation method of the Acellular bone membrane material in natural tissues source | |
| Poongodi et al. | Bio-scaffolds as cell or exosome carriers for nerve injury repair | |
| CN104548205A (en) | Animal-derived nerve scaffold and preparation method thereof | |
| CN103263308B (en) | Many micropores degradable collagen-chitin nerve trachea and preparation method thereof | |
| CN104307044B (en) | A kind of preparation method of the de-cell material of total spinal disc in natural tissues source | |
| CN204394742U (en) | A kind of real core Nerve Scaffold of built-in degradable metal silk | |
| CN101690830B (en) | Preparation method of bionic cartilage extracellular matrix for tissue engineering | |
| CN106236326A (en) | Megastage Bone Defect Repari system | |
| CN104587526A (en) | Collagen-hydroxyapatite nerve scaffold and preparation method thereof | |
| CN105944150B (en) | A kind of hydrogel and the artificial skin and preparation method thereof using the hydrogel | |
| US20160166733A1 (en) | Engineered neural tissue | |
| CN104399130B (en) | A kind of porous cell free tissue engineering bone/cartilage support and preparation method | |
| CN101856515A (en) | Method for preparing artificial bone from chitosan and shell powder serving as raw materials | |
| CN106166307A (en) | A kind of 3D tissue engineering product containing living cells and preparation method thereof | |
| CN104548203B (en) | Preparation method of collagen-rich artificial nerve scaffold and thereof | |
| CN104645409A (en) | Polylactic acid nerve scaffold and preparation method thereof | |
| CN104524628A (en) | Hydroxyapatite nerve scaffold and preparation method thereof | |
| CN106913908A (en) | A kind of cell growth support with structure memory characteristic | |
| RU2016137291A (en) | IMPLANT CONTAINING FGF-18 | |
| CN104524633A (en) | Muscle-based nerve scaffold and preparation method thereof | |
| CN209519145U (en) | Needle holder being applied to surgery Minimally nerve trachea | |
| CN113694253A (en) | Preparation method of small-caliber artificial blood vessel | |
| CN105709276B (en) | A kind of chorion de-cell liquid and method for removing cells |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant |