CN104645409B - A kind of PLA Nerve Scaffold and preparation method thereof - Google Patents
A kind of PLA Nerve Scaffold and preparation method thereof Download PDFInfo
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- CN104645409B CN104645409B CN201410833446.2A CN201410833446A CN104645409B CN 104645409 B CN104645409 B CN 104645409B CN 201410833446 A CN201410833446 A CN 201410833446A CN 104645409 B CN104645409 B CN 104645409B
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- pla
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
- A61L27/04—Metals or alloys
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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Abstract
The invention provides a kind of PLA Nerve Scaffold, including support coating materials and support coating materials parcel degradable metal silk, the degradable metal silk along the support horizontal and vertical distribution, the main component of the support coating materials is PLA.Artificial neuron support prepared by the method is filled with fibronectin splicing variants on PLA, so that schwann cell can grow on support, biodegradable, with the three-dimensional structure required for nerve regneration, has far-reaching significance to CO2 laser weld.
Description
Technical field
The present invention relates to Nerve Scaffold technical field, in particular it relates to a kind of PLA Nerve Scaffold and preparation method thereof.
Background technology
Because peripheral nerve injury caused by various traumatic origins or fracture cause the wounded to feel the decline with motor function
Or lose.Serious peripheral nerve injury often leads to patient's paralysis or permanent loss labour.Peripheral nerve injury is in China
The incidence of disease is higher, and according to statistics, in traumatic patient, four limbs injured nerves account for the 10% of wound sum, be there are about in gunshot fracture
60% merging injured nerves.Therefore, need to find preferable peripheral nerve hinders treatment means.Damaged for peripheral nerve fracture class,
If fracture breach is larger, just must can just make the peripheral nerve of fracture obtain regeneration to repair by bridge joint transfer operation is carried out to breach
It is multiple.
At present, conventional transplant has autotransplantation body and artificial neuron support.Autotransplantation body limited source and meeting band
Carry out donor sequelae.Artificial neuron support aspect, because non-degradable tube material may cause neurothlipsis and second operation
Risk, the main R and D for being intended to Biodegradable scaffold material of Recent study.Currently, the U.S. has been approved by many
Kind of brand, e.g., NeuraGen, SaluBridge, the nerve trachea of Neurolac etc. are used for clinic.However, China props up in nerve
Research in terms of frame more falls behind, and the nerve trachea with independent intellectual property right is not developed.
PLA is a kind of polyester material, is the most the most frequently used material applied in current organizational project, with good
Biocompatibility, degradability, and being had no toxic side effect to human body.However, the cellular affinity of PLA can be poor, poly- breast is used
After the 3 D stereo neural tissue engineering stenter to implant human body of acid construct, the growth that schwann cell can not be good on support, because
And, limit application of the PLA on neural tissue engineering support.
The content of the invention
In order to overcome the deficiencies in the prior art, the invention provides a kind of PLA Nerve Scaffold and preparation method thereof, should
Artificial neuron support prepared by method is filled with fibronectin splicing variants on PLA, so that schwann cell can be in support
Upper growth, biodegradable, with the three-dimensional structure required for nerve regneration, has far-reaching significance to CO2 laser weld.
Technical scheme is as follows：A kind of PLA Nerve Scaffold, including support coating materials and support coating materials parcel
Degradable metal silk, the main component of the support coating materials is PLA, and fiber adhesion egg is stained with the support coating materials
In vain, degradable metal silk along the support horizontal and vertical distribution, wherein the preparation side of the PLA Nerve Scaffold
Method, comprises the following steps：
1) PDLLA R206 is taken, its molecular weight 50X103, the dioxane for being dissolved in 5% obtains PLA
R206 dioxane solutions, are then transferred in the mold cavity of masking mould, along the transverse direction in rack forming direction in mold cavity
There is degradable metal silk with placed longitudinally, mould is then immersed into 2h in liquid nitrogen, taking-up is placed under 0 DEG C of vacuum environment and allows solvent
Preliminary volatilization, then allow solvent thoroughly to volatilize at room temperature so that PLA forms support coating materials and wraps up the degradable metal
Silk, the i.e. precursor of PLA Nerve Scaffold；
2) precursor of the PLA Nerve Scaffold of gained is trimmed to the strip of 10-30mm, with the fibre of 30-80ug/ml
Dimension Fibronectin solution is irrigated from strip top, fibronectin splicing variants solution is flowed down along the hole in strip, static 0.5-
3h, the lower sterilization 15-35min of ultraviolet irradiation, obtains the PLA Nerve Scaffold.
The support is sheet, strip or cylindric.
The main component of the degradable metal silk is magnesium, zinc, calcium, iron or its alloy.
The three-dimensional structure of the polylactic acid bracket is stringer tubular arrangement, and aperture is 10-100 μm, and this structure is beneficial to thin
Born of the same parents regenerate.Irrigate fibronectin splicing variants in the support coating materials of PLA row, contribute to schwann cell between on grow so that
Be conducive to nerve regneration.
Degradable metal and its alloy can guide nerve growth, so as to be conducive to the reparation of nerve cell.
Beneficial effects of the present invention are：PLA Nerve Scaffold of the present invention is filled with fiber adhesion egg on PLA
In vain, so that schwann cell can grow on support, biodegradable, with the three-dimensional structure required for nerve regneration,
And degradable metal silk guiding nerve growth in support is provided with, CO2 laser weld is had far-reaching significance.
Brief description of the drawings：
Fig. 1 is the structural representation of PLA Nerve Scaffold of the present invention.
Specific embodiment
The invention will be further described with preferred embodiment below in conjunction with the accompanying drawings, but embodiments of the present invention are not limited
In this.
The degradable metal of reference picture 1, a kind of PLA Nerve Scaffold, including support coating materials 1 and the parcel of support coating materials 1
Silk 2.Degradable metal silk 2 along the support horizontal and vertical distribution.The support can according to using need prepare
Into variously-shaped, generally cylindrical cross section, or sheet.
The main component of the support coating materials 1 is PLA, and the main component of degradable metal silk 2 is magnesium, zinc,
Calcium, iron or its alloy.
Embodiment 1：A kind of preparation method between PLA nerve, comprises the following specific steps that：
1) PDLLA R206 is taken, its molecular weight 50X103, the dioxane for being dissolved in 5% obtains PLA
R206 dioxane solutions, are then transferred in the mold cavity of masking mould, along the transverse direction in rack forming direction in mold cavity
There is degradable metal silk with placed longitudinally, mould is then immersed into 2h in liquid nitrogen, taking-up is placed under 0 DEG C of vacuum environment and allows solvent
Preliminary volatilization, then allow solvent thoroughly to volatilize at room temperature so that PLA forms support coating materials and wraps up the degradable metal
Silk, the i.e. precursor of PLA Nerve Scaffold；
2) precursor of the PLA Nerve Scaffold of gained is trimmed to the strip of 20mm, with the fiber adhesion egg of 50ug/ml
White solution is irrigated from strip top, fibronectin splicing variants solution is flowed down along the hole in strip, static 1.5h, ultraviolet irradiation
Lower sterilization 30min, obtains the PLA Nerve Scaffold.
Embodiment 2：A kind of preparation method between PLA nerve, comprises the following specific steps that：
1) PLA is taken, its molecular weight 50X103, the dioxane for being dissolved in 5% obtains PLA dioxane second
Alkane solution, is then transferred in the mold cavity of masking mould, and horizontal and vertical being placed with mold cavity along rack forming direction can
Degraded wire, then immerses 2h in liquid nitrogen by mould, and taking-up allows solvent tentatively to volatilize under being placed in 0 DEG C of vacuum environment, then
Solvent is allowed thoroughly to volatilize at room temperature so that PLA forms support coating materials and wraps up the degradable metal silk, i.e. PLA god
Through the precursor of support；
2) precursor of the PLA Nerve Scaffold of gained is trimmed to the strip of 10mm, with the fiber adhesion egg of 30ug/ml
White solution is irrigated from strip top, fibronectin splicing variants solution is flowed down along the hole in strip, static 3h, under ultraviolet irradiation
Sterilization 15min, obtains the PLA Nerve Scaffold.
Above content is to combine specific preferred embodiment further description made for the present invention, it is impossible to assert
Specific implementation of the invention is confined to these explanations.For general technical staff of the technical field of the invention,
On the premise of not departing from present inventive concept, its framework form can be flexible and changeable, can be with subseries product.Simply make some
Simple deduction or replace, should all be considered as belonging to the scope of patent protection that the claims of the present invention by being submitted to determine.
Claims (3)
1. a kind of PLA Nerve Scaffold, it is characterised in that including the degradable metal that support coating materials and support coating materials are wrapped up
Silk, the main component of the support coating materials is PLA, and fibronectin splicing variants are stained with the support coating materials, described degradable
Wire along the support horizontal and vertical distribution, wherein the preparation method of the PLA Nerve Scaffold, including following step
Suddenly：
1) PDLLA R206 is taken, its molecular weight 50X103, the dioxane for being dissolved in 5% obtains PLA R206 bis-
Oxa- cyclohexane solution, is then transferred in the mold cavity of masking mould, along the horizontal and vertical of rack forming direction in mold cavity
Degradable metal silk is placed with, mould is then immersed into 2h in liquid nitrogen, taking-up allows solvent tentatively to wave under being placed in 0 DEG C of vacuum environment
Hair, then allow solvent thoroughly to volatilize at room temperature so that PLA forms support coating materials and wraps up the degradable metal silk, that is, gather
The precursor of lactic acid Nerve Scaffold；
2) precursor of the PLA Nerve Scaffold of gained is trimmed to the strip of 10-30mm, is glued with the fiber of 30-80 μ g/ml
Even protein solution is irrigated from strip top, fibronectin splicing variants solution is flowed down along the hole in strip, static 0.5-3h, purple
The lower sterilization 15-35min of outside line irradiation, obtains the PLA Nerve Scaffold.
2. PLA Nerve Scaffold as claimed in claim 1, it is characterised in that the support is sheet, strip or cylinder
Shape.
3. PLA Nerve Scaffold as claimed in claim 1, it is characterised in that the main component of the degradable metal silk is
Magnesium, zinc, calcium, iron or its alloy.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410833446.2A CN104645409B (en) | 2014-12-29 | 2014-12-29 | A kind of PLA Nerve Scaffold and preparation method thereof |
| PCT/CN2015/082159 WO2016107108A1 (en) | 2014-12-29 | 2015-06-24 | Polylactic acid nerve scaffold and manufacturing method therefor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410833446.2A CN104645409B (en) | 2014-12-29 | 2014-12-29 | A kind of PLA Nerve Scaffold and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104645409A CN104645409A (en) | 2015-05-27 |
| CN104645409B true CN104645409B (en) | 2017-06-23 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410833446.2A Active CN104645409B (en) | 2014-12-29 | 2014-12-29 | A kind of PLA Nerve Scaffold and preparation method thereof |
Country Status (2)
| Country | Link |
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| CN (1) | CN104645409B (en) |
| WO (1) | WO2016107108A1 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104645409B (en) * | 2014-12-29 | 2017-06-23 | 东莞颠覆产品设计有限公司 | A kind of PLA Nerve Scaffold and preparation method thereof |
| CN204394742U (en) * | 2014-12-29 | 2015-06-17 | 东莞颠覆产品设计有限公司 | A kind of real core Nerve Scaffold of built-in degradable metal silk |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1293924C (en) * | 2003-09-02 | 2007-01-10 | 中国人民解放军第四军医大学口腔医学院 | Tissue engineering peripheral nerve used for repairing peripheral nerve defect and its preparation method |
| CN1241656C (en) * | 2004-03-16 | 2006-02-15 | 武汉理工大学 | Aretificial nerve canula material of slow operating complex type poly lactic acid and preparation method |
| TWI374037B (en) * | 2008-08-21 | 2012-10-11 | Univ Taipei Medical | Bio-acceptable conduits and method providing the same |
| US11305501B2 (en) * | 2009-06-25 | 2022-04-19 | Beijing Advanced Medical Technologies, Co., Ltd. | Methods and apparatus for fabricating porous three-dimensional tubular scaffolds |
| CN104107096B (en) * | 2014-07-18 | 2016-09-07 | 上海交通大学 | Flexible degradable magnesium alloy nerve trachea and preparation method thereof |
| CN104645409B (en) * | 2014-12-29 | 2017-06-23 | 东莞颠覆产品设计有限公司 | A kind of PLA Nerve Scaffold and preparation method thereof |
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2014
- 2014-12-29 CN CN201410833446.2A patent/CN104645409B/en active Active
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2015
- 2015-06-24 WO PCT/CN2015/082159 patent/WO2016107108A1/en active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| CN104645409A (en) | 2015-05-27 |
| WO2016107108A1 (en) | 2016-07-07 |
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