CN104524628B - A kind of hydroxyapatite Nerve Scaffold and preparation method thereof - Google Patents
A kind of hydroxyapatite Nerve Scaffold and preparation method thereof Download PDFInfo
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- CN104524628B CN104524628B CN201410834302.9A CN201410834302A CN104524628B CN 104524628 B CN104524628 B CN 104524628B CN 201410834302 A CN201410834302 A CN 201410834302A CN 104524628 B CN104524628 B CN 104524628B
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- hydroxyapatite
- coating materials
- support coating
- nerve scaffold
- nerve
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
- A61L27/04—Metals or alloys
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
- A61L27/12—Phosphorus-containing materials, e.g. apatite
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Transplantation (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
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- Inorganic Chemistry (AREA)
- Materials For Medical Uses (AREA)
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Abstract
The invention provides a kind of hydroxyapatite Nerve Scaffold, the degradable metal silk wrapped up including support coating materials and support coating materials, described support coating materials be mainly composed of hydroxyapatite.Artificial neuron support good mechanical properties prepared by the method, biodegradable, there is the three dimensional structure required for neuranagenesis, CO2 laser weld is had profound significance.
Description
Technical field
The present invention relates to Nerve Scaffold technical field, in particular it relates to a kind of hydroxyapatite Nerve Scaffold and
Its preparation method.
Background technology
The peripheral nerve injury caused due to various traumatic origin or fracture cause the wounded to feel and motor function
Decline or lose.Serious peripheral nerve injury often leads to patient's paralysis or permanent loss labour force.Periphery
Nerve injury is higher at China's sickness rate, and according to statistics, in traumatic patient, it is total that extremity injured nerves accounts for wound
The 10% of number, there are about the merging injured nerves of 60% in gunshot fracture.To this end, preferable periphery god need to be found
Through hindering treatment means.For peripheral nervous fracture class damage, if fracture breach is relatively big, just must be by breach
Carrying out bridging transplant operation just can make the peripheral nerve of fracture obtain Regeneration and Repair.
At present, conventional transplant has autotransplantation body and artificial neuron support.Autotransplantation body limited source
And donor sequela can be brought.Artificial neuron support aspect, owing to non-degradable tube material may cause nerve
Compressing and the risk of second operation, the exploitation mainly trending towards Biodegradable scaffold material of Recent study
And research.Currently, the U.S. has been approved by multiple brand, e.g., NeuraGen, SaluBridge, Neurolac
Deng nerve trachea for clinic.But, China's research in terms of Nerve Scaffold is the most backward, not
Develop the nerve trachea with independent intellectual property right.
Hydroxyapatite is human body and animal body skeleton, the main inorganic composition of tooth, and its physical chemistry forms
Very much like with the inorganic composition of human bone, there is good biocompatibility, biological activity and bone conductibility,
And with body bone tissue, there is fabulous chemistry and bioaffinity.Biological activity prepared by hydroxyapatite
Pottery is inserted internal the most safe and nontoxic, moreover it is possible to conduction osteogenesis.But, it is used for making by hydroxyapatite
Standby Nerve Scaffold, for CO2 laser weld, yet there are no report.
Summary of the invention
In order to overcome the deficiencies in the prior art, the invention provides a kind of hydroxyapatite Nerve Scaffold and system thereof
Preparation Method, Nerve Scaffold good mechanical properties prepared by the method, biodegradable, there is neuranagenesis institute
The three dimensional structure needed, has profound significance to CO2 laser weld.
Technical scheme is as follows: a kind of hydroxyapatite Nerve Scaffold, including support coating materials and
Frame coating materials parcel degradable metal silk, described support coating materials be mainly composed of hydroxyapatite.
Described support is lamellar, strip or cylindric.
Described degradable metal silk is along the horizontal and vertical distribution of described support.
Described degradable metal silk be mainly composed of magnesium, zinc, calcium, ferrum or its alloy.
The preparation method of described hydroxyapatite Nerve Scaffold, comprises the steps:
1) binding agent is prepared: weigh 3g carboxymethyl cellulose and be dissolved in 60ml distilled water, use temperature control magnetic force
Agitator be heated to 40 DEG C and stir 30min to solution clarify, obtain cmc soln；
2) prepare hydroxyapatite slurry: take hydroxyapatite powder, calcine 3 hours at 900 DEG C, cooling
After be dissolved in distilled water, use supersonic oscillations 30min so that it is be uniformly dispersed, obtain hydroxyapatite slurry,
Its mass concentration is 10-50%；
3) support coating materials is prepared: in step 2) gained hydroxyapatite slurry adds the step of 1-1.5% volume
Rapid 1 gained cmc soln, uses magnetic stirrer 1h, stands 24h, slough slurry in vacuum tank
Gas in material, obtains described support coating materials；
4) described support coating materials is injected in the die cavity of special die, along support coating materials forming direction in die cavity
Horizontal and vertical degradable metal silk is set ,-10--70 DEG C of freezing, then mould is taken out and put into sucking filtration and set
Standby, and connect vacuum pump, it is placed in refrigerator-freezer and carries out evacuation and be dried；Then, at 200-400 DEG C of high temperature binder removal,
Remove carboxymethyl cellulose, then sinter 2-6 hour at 1000-1200 DEG C, obtain described hydroxyapatite nerve and prop up
Frame.
The porosity of described Nerve Scaffold is 40-65%.
The invention have the benefit that hydroxyapatite Nerve Scaffold good mechanical properties of the present invention, raw
Biodegradable, has the three dimensional structure required for neuranagenesis, CO2 laser weld is had profound significance.
Accompanying drawing illustrates:
Fig. 1 is the structural representation of hydroxyapatite Nerve Scaffold of the present invention.
Fig. 2 a, 2b and 2c are the upper surface obtaining hydroxyapatite Nerve Scaffold with observation station under microscope, following table
Face and longitudinal surface photo.
Detailed description of the invention
The invention will be further described with preferred embodiment below in conjunction with the accompanying drawings, but the embodiment party of the present invention
Formula is not limited to this.
With reference to Fig. 1, a kind of hydroxyapatite Nerve Scaffold, wraps up including support coating materials 1 and support coating materials 1
Degradable metal silk 2.Described degradable metal silk is along the horizontal and vertical distribution of described support.Described support
Can need to be prepared as according to use variously-shaped, generally cylindrical cross section, it is also possible to be lamellar.Described nerve
The porosity of support is 40-65%.
Described support coating materials 1 is hydroxyapatite.Described degradable metal silk 2 be mainly composed of magnesium, zinc,
Calcium, ferrum or its alloy.
Embodiment 1, the preparation method of a kind of hydroxyapatite Nerve Scaffold, comprise the steps:
1) binding agent is prepared: weigh carboxymethyl cellulose and be dissolved in distilled water, add with temperature control magnetic stirring apparatus
Heat to 40 DEG C and stir 30min to solution clarify, obtain cmc soln, its mass concentration is 5%；
2) prepare hydroxyapatite slurry: take hydroxyapatite powder, calcine 3 hours at 900 DEG C, cooling
After be dissolved in distilled water, use supersonic oscillations 30min so that it is be uniformly dispersed, obtain hydroxyapatite slurry,
Its mass concentration is 40%；
3) support coating materials is prepared: in step 2) gained hydroxyapatite slurry adds the step of 1.5% volume
1 gained cmc soln, uses magnetic stirrer 1h, stands 24h, slough in slurry in vacuum tank
Gas, obtain described support coating materials；
4) described support coating materials is injected in the die cavity of special die, along support coating materials forming direction in die cavity
Horizontal and vertical degradable metal silk is set ,-10 DEG C of freezings, then mould is taken out and put into pumping and filtering device,
And connect vacuum pump, it is placed in refrigerator-freezer and carries out evacuation and be dried；Then, at 200-400 DEG C of high temperature binder removal, remove
Remove carboxymethyl cellulose, then sinter 2-6 hour at 1050 DEG C, obtain described hydroxyapatite Nerve Scaffold.
Fig. 2 a, 2b and 2c are the upper surface obtaining artificial neuron support with observation station under microscope, and lower surface is with vertical
To surface picture.
Embodiment 2, the preparation method of a kind of hydroxyapatite Nerve Scaffold, comprise the steps:
1) binding agent is prepared: weigh carboxymethyl cellulose and be dissolved in distilled water, add with temperature control magnetic stirring apparatus
Heat to 40 DEG C and stir 30min to solution clarify, obtain cmc soln, its mass concentration is 3%；
2) prepare hydroxyapatite slurry: take hydroxyapatite powder, calcine 3 hours at 800-DEG C, cooling
After be dissolved in distilled water, use supersonic oscillations 40min so that it is be uniformly dispersed, obtain hydroxyapatite slurry,
Its mass concentration is 50%；
3) support coating materials is prepared: in step 2) gained hydroxyapatite slurry adds the step 1 of 1% volume
Gained cmc soln, uses magnetic stirrer 1h, stands 24h, slough in slurry in vacuum tank
Gas, obtain described support coating materials；
4) described support coating materials is injected in the die cavity of special die, along support coating materials forming direction in die cavity
Horizontal and vertical degradable metal silk is set ,-70 DEG C of freezings, then mould is taken out and put into pumping and filtering device,
And connect vacuum pump, it is placed in refrigerator-freezer and carries out evacuation and be dried；Then, at 200-400 DEG C of high temperature binder removal, remove
Remove carboxymethyl cellulose, then sinter 2-6 hour at 1000-1200 DEG C, obtain described hydroxyapatite nerve and prop up
Frame.
Above content is to combine concrete preferred implementation further description made for the present invention, no
Can assert the present invention be embodied as be confined to these explanations.Common for the technical field of the invention
For technical staff, without departing from the inventive concept of the premise, its framework form can be flexible and changeable, can
With subseries product.Simply make some simple deduction or replace, all should be considered as belonging to the present invention by institute
The scope of patent protection that the claims submitted to determine.
Claims (5)
1. a hydroxyapatite Nerve Scaffold, it is characterised in that include support coating materials and support coating materials bag
The degradable metal silk wrapped up in, described support coating materials be mainly composed of hydroxyapatite；Wherein said hydroxyl phosphorus
The preparation method of lime stone Nerve Scaffold comprises the steps:
1) binding agent is prepared: weigh 3g carboxymethyl cellulose and be dissolved in 60ml distilled water, use temperature control magnetic force
Agitator be heated to 40 DEG C and stir 30min to solution clarify, obtain cmc soln；
2) prepare hydroxyapatite slurry: take hydroxyapatite powder, calcine 3 hours at 900 DEG C, cooling
After be dissolved in distilled water, use supersonic oscillations 30min so that it is be uniformly dispersed, obtain hydroxyapatite slurry,
Its mass concentration is 10-50%；
3) support coating materials is prepared: in step 2) gained hydroxyapatite slurry adds the step of 1-1.5% volume
Rapid 1 gained cmc soln, uses magnetic stirrer 1h, stands 24h, slough slurry in vacuum tank
Gas in material, obtains described support coating materials；
4) described support coating materials is injected in the die cavity of special die, along support coating materials forming direction in die cavity
Horizontal and vertical degradable metal silk is set ,-10--70 DEG C of freezing, then mould is taken out and put into sucking filtration and set
Standby, and connect vacuum pump, it is placed in refrigerator-freezer and carries out evacuation and be dried；Then, at 200-400 DEG C of high temperature binder removal,
Remove carboxymethyl cellulose, then sinter 2-6 hour at 1000-1200 DEG C, obtain described hydroxyapatite nerve and prop up
Frame.
2. hydroxyapatite Nerve Scaffold as claimed in claim 1, it is characterised in that described support is sheet
Shape, strip or cylindric.
3. hydroxyapatite Nerve Scaffold as claimed in claim 1, it is characterised in that described degradable gold
Belong to the silk horizontal and vertical distribution along described support.
4. hydroxyapatite Nerve Scaffold as claimed in claim 1, it is characterised in that described degradable gold
Belong to silk is mainly composed of magnesium, zinc, calcium, ferrum or its alloy.
5. hydroxyapatite Nerve Scaffold as claimed in claim 1, it is characterised in that described Nerve Scaffold
Porosity be 40-65%.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410834302.9A CN104524628B (en) | 2014-12-29 | 2014-12-29 | A kind of hydroxyapatite Nerve Scaffold and preparation method thereof |
| PCT/CN2015/082175 WO2016107110A1 (en) | 2014-12-29 | 2015-06-24 | Hydroxyapatite nerve scaffold and manufacturing method therefor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410834302.9A CN104524628B (en) | 2014-12-29 | 2014-12-29 | A kind of hydroxyapatite Nerve Scaffold and preparation method thereof |
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| Publication Number | Publication Date |
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| CN104524628A CN104524628A (en) | 2015-04-22 |
| CN104524628B true CN104524628B (en) | 2016-11-02 |
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| CN204394742U (en) * | 2014-12-29 | 2015-06-17 | 东莞颠覆产品设计有限公司 | A kind of real core Nerve Scaffold of built-in degradable metal silk |
| CN104524628B (en) * | 2014-12-29 | 2016-11-02 | 东莞颠覆产品设计有限公司 | A kind of hydroxyapatite Nerve Scaffold and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1241656C (en) * | 2004-03-16 | 2006-02-15 | 武汉理工大学 | Aretificial nerve canula material of slow operating complex type poly lactic acid and preparation method |
| TWI374037B (en) * | 2008-08-21 | 2012-10-11 | Univ Taipei Medical | Bio-acceptable conduits and method providing the same |
| CN102149859B (en) * | 2009-06-25 | 2015-08-26 | 北京阿迈特医疗器械有限公司 | For the preparation of the method and apparatus of three-dimensional porous tubular bracket |
| CN102000362A (en) * | 2010-12-02 | 2011-04-06 | 上海纳米技术及应用国家工程研究中心有限公司 | Silk fibroin/nano hydroxyapatite porous bracket material and preparation method thereof |
| CN104107096B (en) * | 2014-07-18 | 2016-09-07 | 上海交通大学 | Flexible degradable magnesium alloy nerve trachea and preparation method thereof |
| CN104524628B (en) * | 2014-12-29 | 2016-11-02 | 东莞颠覆产品设计有限公司 | A kind of hydroxyapatite Nerve Scaffold and preparation method thereof |
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| WO2016107110A1 (en) | 2016-07-07 |
| CN104524628A (en) | 2015-04-22 |
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