CN104543565B - A kind of spotted maigre compound feed for young and preparation method thereof - Google Patents

A kind of spotted maigre compound feed for young and preparation method thereof Download PDF

Info

Publication number
CN104543565B
CN104543565B CN201510013943.2A CN201510013943A CN104543565B CN 104543565 B CN104543565 B CN 104543565B CN 201510013943 A CN201510013943 A CN 201510013943A CN 104543565 B CN104543565 B CN 104543565B
Authority
CN
China
Prior art keywords
parts
spotted maigre
feed
young
fish
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201510013943.2A
Other languages
Chinese (zh)
Other versions
CN104543565A (en
Inventor
王立改
楼宝
詹炜
陈睿毅
毛国民
徐冬冬
徐麒翔
马涛
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zhejiang Marine Fisheries Research Institute
Original Assignee
Zhejiang Marine Fisheries Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zhejiang Marine Fisheries Research Institute filed Critical Zhejiang Marine Fisheries Research Institute
Priority to CN201510013943.2A priority Critical patent/CN104543565B/en
Publication of CN104543565A publication Critical patent/CN104543565A/en
Application granted granted Critical
Publication of CN104543565B publication Critical patent/CN104543565B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

Landscapes

  • Feed For Specific Animals (AREA)
  • Fodder In General (AREA)

Abstract

A kind of spotted maigre compound feed for young and preparation method thereof, by fish meal, dregs of beans, gluten powder, fish oil, dextrin, soybean lecithin, multidimensional premix, many ore deposit premixes, Choline Chloride, calcium dihydrogen phosphate, composite immune reinforcing agent, cellulose composition, the feed formula that the present invention designs for the nutritional need specifically designed for spotted maigre juvenile fish, feature of ingesting and digestive characteristic, disclosure satisfy that whole nutrient demands needed for the development of spotted maigre juvenile growth.Feed formulation nutrients composition of the present invention is comprehensively balanced, and environmental protection, can be good in water stability.Prove that feed of the present invention can significantly improve spotted maigre rate of body weight gain and survival rate by culture experiment, improve spotted maigre premunition.

Description

A kind of spotted maigre compound feed for young and preparation method thereof
Technical field:
The present invention relates to a kind of agriculture mariculture technology field, and in particular to a kind of spotted maigre compound feed for young and its Preparation method.
Background technology:
Spotted maigre(Nibea albiflora Richardson), it is subordinate to Perciformes, Sciaenidae, Nibea warms up temperature Lower floor fish in property coastal waters, are distributed mainly on northwest Pacific area, the South Sea, the East Sea and the Huanghai Sea of China are distributed.Its meat flavour Rapidly, disease is few for delicious, nutritious and growth, and cultivation can reach marketable fish listing specification then, it has also become seawater fishnet Cage breeding grows extremely potential new varieties.But lack the cooperation feeding individually for feature research and development of spotted maigre juvenile fish itself at present Material.
, can only be to feed based on chilled small fish and other fingerling mixed feeds in current spotted maigre cultivation.Feed Not only feed coefficient is higher for small fish, and easily brings pathogen into, pollutes breeding environment, in addition chilled small fish source of goods by Closed fishing season and the restriction in season, the freshness and quality of fish are relatively difficult to ensure card;And the mixed feed of other fingerlings is fed due to its nutrition Parameter is not specific to spotted maigre juvenile fish and set, so causing the growth of spotted maigre and the rate of body weight gain can not to reach it most Good growth conditions.With the continuous expansion of spotted maigre aquaculture, the high-quality of its needs that grow can be met in the urgent need to providing Mixed feed, therefore need the special compound feed developed specifically designed for spotted maigre feature itself badly, to accelerate spotted maigre industrialization Process.
The content of the invention:
It is an object of the invention to provide agriculture mariculture technology, the present invention is the nutrition specifically designed for spotted maigre juvenile fish The feed formula that demand, feature of ingesting and digestive characteristic are designed, disclosure satisfy that spotted maigre juvenile growth development needed for it is complete Portion's nutrient demand.Feed formulation nutrients composition of the present invention is comprehensively balanced, and environmental protection, in water stability Can be good.Prove that feed of the present invention can significantly improve spotted maigre rate of body weight gain and survival rate by culture experiment, improve spotted maigre and resist Sick power.
The technical solution that the present invention is used is:A kind of spotted maigre compound feed for young, by the original of following parts by weight Material is made:30-45 parts of fish meal, 15-25 parts of dregs of beans, 8-16 parts of gluten powder, 5-15 parts of fish oil, 5-13 parts of dextrin, soybean ovum - 3 parts of phosphatidase 1,0.1-0.8 parts of multidimensional premix, many 0.1-0.8 parts of ore deposit premixes, 0.1-0.6 parts of Choline Chloride, biphosphate 0.1-0.8 parts of calcium, 0.05-0.4 parts of composite immune reinforcing agent, 5-15 parts of cellulose;Described composite immune reinforcing agent is β-Portugal Glycan, A3 α-peptide glycan.
A kind of described spotted maigre compound feed for young, is prepared from the following raw materials in parts by weight:35-40 parts of fish meal, beans 18-22 parts of the dregs of rice, 10-15 parts of gluten powder, 7-10 parts of fish oil, 7-9 parts of dextrin, 1-2 parts of soybean lecithin, multidimensional premix 0.2-0.6 parts, many 0.2-0.5 parts of ore deposit premixes, 0.2-0.5 parts of Choline Chloride, 0.2-0.6 parts of calcium dihydrogen phosphate, complex immunity 0.1-0.3 parts of reinforcing agent, 7-13 parts of cellulose;Described composite immune reinforcing agent is beta glucan, A3 α-peptide glycan.
A kind of described spotted maigre compound feed for young, is prepared from the following raw materials in parts by weight:37 parts of fish meal, dregs of beans 20 Part, 12 parts of gluten powder, 8.5 parts of fish oil, 8 parts of dextrin, 1.5 parts of soybean lecithin, 0.5 part of multidimensional premix, many ore deposit premixs 0.3 part of material, 0.3 part of Choline Chloride, 0.5 part of calcium dihydrogen phosphate, 0.25 part of composite immune reinforcing agent, 11.15 parts of cellulose;It is described Composite immune reinforcing agent be beta glucan, A3 α-peptide glycan.
A kind of preparation method of described spotted maigre compound feed for young, comprises the following steps:
(1)Solid feed raw material is crushed with pulverizer, and the whole that will grind fodder crosses 60 mesh sieves;
(2)All feedstuffs are weighed by formula rate, and mixed according to the principle expanded step by step, are mixed Time is more than half an hour;
(3)Mixed raw material is put into mixer to add water stirring, when humidity reaches 8%-12%, passes through banded extruder and system Grain machine, is made a diameter of 2mm and 3mm granulated meal;
(4)By the feed chamber fitted's warm air made it is dry after, be fitted into sealed bag, be stored in -20 DEG C it is stand-by.
Multidimensional premix in a kind of raw material of described spotted maigre compound feed for young be characterised by including it is following into Point(Raw work bioengineering(Shanghai)Limited company buys):Vitamine A acetate, vitamine D3, vitamin K, vitamin E, vitamin B1, vitamin B2, vitamin B6, vitamin B12, nicotinic acid, folic acid, biotin, inositol, vitamin C.
Many ore deposit premixes in a kind of raw material of described spotted maigre compound feed for young be characterised by including it is following into Point(Chemical Reagent Co., Ltd., Sinopharm Group buys):Epsom salt, ferrous sulfate heptahydrate, white vitriol, sulfuric acid monohydrate Manganese, CoCL2 6H2O, sodium selenite, KI, cupric sulfate pentahydrate, sodium chloride.
The beneficial effect that the present invention is obtained is:The invention provides a kind of spotted maigre compound feed for young and its preparation side Method, disclosure satisfy that whole nutrient demands needed for the development of spotted maigre juvenile growth.Feed formulation nutrients of the present invention into Point comprehensively balanced, and environmental protection is good in water stability energy.Prove that feed of the present invention can be notable by culture experiment Spotted maigre rate of body weight gain and survival rate are improved, spotted maigre premunition is improved.
Embodiment:
In July, 2014 is prepared for mixed feed in oceanography institute of University Of Ningbo feed process room.The 8-10 months in 2014, in Zhejiang The culture efficiency evaluation test that test site has carried out spotted maigre compound feed for young is dodged in west by marine fishery research institute of river province.Spotted maigre Gunther parent fish spawning that juvenile fish is cultivated from test site, hatching gained.
Example:
One feed formula
Embodiment 1
Feed formula is constituted(Calculated with 1000g):Peru fish meal(Crude protein 64%, crude fat 10%, the biological section of the big health of promise Skill Co., Ltd)370g, dregs of beans(Crude protein 45%, crude fat 1.2%)200g, gluten powder(Crude protein 75%, crude fat 1%, Hua Fengfen industry Co., Ltd)120g, fish oil(EPA>7%,DHA>13%, peroxide value<10meq/kg, the life of Weihai Ai Ersi oceans Thing Science and Technology Ltd.)85g, dextrin 80g, soybean lecithin 15g, multidimensional premix 5g, many ore deposit premix 3g, Choline Chloride 3g, calcium dihydrogen phosphate 5g, composite immune reinforcing agent(Beta glucan+A3 α-peptide glycan)2.5g, cellulose 111.5g.
The feed nutrition made is constituted:Moisture, 8.26%;Crude protein, 49.7%;Crude fat, 12.7%;Coarse ash 11.2%
Embodiment 2
Feed formula is constituted(Calculated with 1000g):Peru fish meal(Crude protein 64%, crude fat 10%, the biological section of the big health of promise Skill Co., Ltd)300g, dregs of beans(Crude protein 45%, crude fat 1.2%)221.5g, gluten powder(Crude protein 75%, crude fat 1%, Hua Fengfen industry Co., Ltd)80g, fish oil(EPA>7%,DHA>13%, peroxide value<10meq/kg, Weihai Ai Ersi oceans Bio tech ltd)150g, dextrin 50g, soybean lecithin 30g, multidimensional premix 1g, many ore deposit premix 8g, chlorination courage Alkali 1g, calcium dihydrogen phosphate 8g, composite immune reinforcing agent(Beta glucan+A3 α-peptide glycan)0.5g, cellulose 150g.
The feed nutrition made is constituted:Moisture, 9.43%;Crude protein, 40.1%;Crude fat, 16.7%;Coarse ash 10.6%
Embodiment 3
Feed formula is constituted(Calculated with 1000g):Peru fish meal(Crude protein 64%, crude fat 10%, the biological section of the big health of promise Skill Co., Ltd)430g, dregs of beans(Crude protein 45%, crude fat 1.2%)150g, gluten powder(Crude protein 75%, crude fat 1%, Hua Fengfen industry Co., Ltd)160g, fish oil(EPA>7%,DHA>13%, peroxide value<10meq/kg, the life of Weihai Ai Ersi oceans Thing Science and Technology Ltd.)50g, dextrin 130g, soybean lecithin 10g, multidimensional premix 8g, many ore deposit premix 1g, Choline Chloride 6g, calcium dihydrogen phosphate 1g, composite immune reinforcing agent(Beta glucan+A3 α-peptide glycan)4g, cellulose 50g.
The feed nutrition made is constituted:Moisture, 10.4%;Crude protein, 54.7%;Crude fat, 9.64%;Coarse ash 13.4%
Two preparation methods
Solid feed raw material is crushed with pulverizer, and the whole that will grind fodder crosses 60 mesh sieves;All feeds is former Material is weighed in the ratio of feed formula, and is mixed according to the principle expanded step by step, and incorporation time is more than half an hour; The sufficient raw material of mixing is put into mixer and added water stirring, when humidity reaches 8%-12%, by banded extruder and granulator, is made A diameter of 2mm and 3mm granulated meal;The feed chamber fitted's warm air made is done(Moisture is below 12%)Afterwards, sealing is loaded In bag, -20 DEG C of refrigerators are stored in stand-by.
Three culture experiments
Experimental subjects is used as using 17.7 ± 0.2g spotted maigre juvenile fish.Spotted maigre juvenile fish indoors temporarily support two weeks by cement pit. Before formal culture experiment, selection specification is close, bouncing juvenile fish is weighed, and puts at random into 500-L Glass fibre reinforced plastic tubs, often 20 tail juvenile fish of bucket, each 3 repetitions of processing.Feed formula example 1,2,3 is fed respectively and carries out contrast test, and control group is fed not Composite immune reinforcing agent group is added, test group adds composite immune reinforcing agent group to feed.Continuous aeration in each experiment bucket, with Keep enough dissolved oxygens.Feed twice daily(The morning 08:00, afternoon 17:00).The water of daily renewal 1/3.In the entirely cultivation phase Between, temperature is maintained at 27 ± 2 DEG C, and pH is 7.8-8.0, and salinity is 28 ‰ -29 ‰.Dissolved oxygen is controlled in more than 6mg/L, and ammonia nitrogen contains Amount is less than 0.02mg/L.Culture-cycle is 56 days, and result of the test shows:The spotted maigre juvenile fish of test group in feed formula example 1 Survival rate is up to 96.7%, and rate of body weight gain is up to 336%, and control group spotted maigre juvenile fish survival rate is that 81% rate of body weight gain is 229%; The spotted maigre juvenile fish survival rate of test group is up to 85.2% in feed formula example 2, and rate of body weight gain is 211%, control group spotted maigre Juvenile fish survival rate is 78%, and rate of body weight gain is 198%;The spotted maigre juvenile fish survival rate of test group is 83.4% in feed formula example 3, Rate of body weight gain is 207%, and control group spotted maigre juvenile fish survival rate is 73%, and rate of body weight gain is 180%.

Claims (4)

1. a kind of spotted maigre compound feed for young, it is characterised in that be prepared from the following raw materials in parts by weight:Fish meal 30-45 Part, 15-25 parts of dregs of beans, 8-16 parts of gluten powder, 5-15 parts of fish oil, 5-13 parts of dextrin, 1-3 parts of soybean lecithin, multidimensional is pre- 0.1-0.8 parts of batch mixing, many 0.1-0.8 parts of ore deposit premixes, 0.1-0.6 parts of Choline Chloride, 0.1-0.8 parts of calcium dihydrogen phosphate is combined 0.05-0.4 parts of immunopotentiator, 5-15 parts of cellulose;Described composite immune reinforcing agent is beta glucan, A3 α-peptide glycan.
2. a kind of spotted maigre compound feed for young according to claim 1, it is characterised in that by the original of following parts by weight Material is made:35-40 parts of fish meal, 18-22 parts of dregs of beans, 10-15 parts of gluten powder, 7-10 parts of fish oil, 7-9 parts of dextrin, soybean ovum - 2 parts of phosphatidase 1,0.2-0.6 parts of multidimensional premix, many 0.2-0.5 parts of ore deposit premixes, 0.2-0.5 parts of Choline Chloride, biphosphate 0.2-0.6 parts of calcium, 0.1-0.3 parts of composite immune reinforcing agent, 7-13 parts of cellulose;Described composite immune reinforcing agent is β-Portugal Glycan, A3 α-peptide glycan.
3. a kind of spotted maigre compound feed for young according to claim 1, it is characterised in that by the original of following parts by weight Material is made:37 parts of fish meal, 20 parts of dregs of beans, 12 parts of gluten powder, 8.5 parts of fish oil, 8 parts of dextrin, 1.5 parts of soybean lecithin is more Tie up 0.5 part of premix, many 0.3 part of ore deposit premixes, 0.3 part of Choline Chloride, 0.5 part of calcium dihydrogen phosphate, composite immune reinforcing agent 0.25 part, 11.2 parts of cellulose;Described composite immune reinforcing agent is beta glucan, A3 α-peptide glycan.
4. a kind of a kind of preparation method of spotted maigre compound feed for young described in claim 1 or 2 or 3, it is characterised in that bag Include following steps:
(1)Solid feed raw material is crushed with pulverizer, and the whole that will grind fodder crosses 60 mesh sieves;
(2)All feedstuffs are weighed by formula rate, and mixed according to the principle expanded step by step, incorporation time More than half an hour;
(3)Mixed raw material is put into mixer to add water stirring, when humidity reaches 8%-12%, passes through banded extruder and granulation Machine, is made a diameter of 2mm and 3mm granulated meal;
(4)By the feed chamber fitted's warm air made it is dry after, be fitted into sealed bag, be stored in -20 DEG C it is stand-by.
CN201510013943.2A 2015-01-13 2015-01-13 A kind of spotted maigre compound feed for young and preparation method thereof Active CN104543565B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510013943.2A CN104543565B (en) 2015-01-13 2015-01-13 A kind of spotted maigre compound feed for young and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201510013943.2A CN104543565B (en) 2015-01-13 2015-01-13 A kind of spotted maigre compound feed for young and preparation method thereof

Publications (2)

Publication Number Publication Date
CN104543565A CN104543565A (en) 2015-04-29
CN104543565B true CN104543565B (en) 2017-07-11

Family

ID=53061489

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201510013943.2A Active CN104543565B (en) 2015-01-13 2015-01-13 A kind of spotted maigre compound feed for young and preparation method thereof

Country Status (1)

Country Link
CN (1) CN104543565B (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104996343A (en) * 2015-08-12 2015-10-28 张晶 Method for culturing nibea albiflora
CN106562109A (en) * 2016-11-02 2017-04-19 通化师范学院 Environmentally friendly soft pellet feed for breeding freshwater salmon, and preparation method and use thereof
CN106942522A (en) * 2017-03-17 2017-07-14 浙江省海洋水产研究所 A kind of spotted maigre juvenile fish vitamin premix and preparation method thereof
CN107373206A (en) * 2017-07-29 2017-11-24 合肥申仁养殖有限公司 A kind of spotted maigre juvenile fish special feed
CN107373208A (en) * 2017-07-29 2017-11-24 合肥申仁养殖有限公司 A kind of mixed feed for promoting spotted maigre growth
CN108813209A (en) * 2018-06-04 2018-11-16 浙江省海洋水产研究所 A kind of little yellow croaker young stage feed and preparation method thereof
CN108850734A (en) * 2018-09-03 2018-11-23 汕头大学 It is a kind of improve Nibea coibor air bladder quality artifical compound feed and its preparation

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101473899A (en) * 2008-12-30 2009-07-08 浙江海洋学院 Compound feed for Nibea japonica
CN101480488A (en) * 2008-11-19 2009-07-15 广东海洋大学 Complex immunopotentiator for grouper
CN103070301A (en) * 2012-12-21 2013-05-01 浙江海洋学院 Basic bait formulation for Nibea albiflora
CN103355495A (en) * 2012-05-21 2013-10-23 青岛玛斯特生物技术有限公司 Functional composite additive premix for freshwater fish
CN104000009A (en) * 2014-02-24 2014-08-27 舟山海洋科技有限公司 Nibea albiflora feed and preparing method of nibea albiflora feed

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101480488A (en) * 2008-11-19 2009-07-15 广东海洋大学 Complex immunopotentiator for grouper
CN101473899A (en) * 2008-12-30 2009-07-08 浙江海洋学院 Compound feed for Nibea japonica
CN103355495A (en) * 2012-05-21 2013-10-23 青岛玛斯特生物技术有限公司 Functional composite additive premix for freshwater fish
CN103070301A (en) * 2012-12-21 2013-05-01 浙江海洋学院 Basic bait formulation for Nibea albiflora
CN104000009A (en) * 2014-02-24 2014-08-27 舟山海洋科技有限公司 Nibea albiflora feed and preparing method of nibea albiflora feed

Also Published As

Publication number Publication date
CN104543565A (en) 2015-04-29

Similar Documents

Publication Publication Date Title
CN104543565B (en) A kind of spotted maigre compound feed for young and preparation method thereof
CN102845653B (en) Nibea albiflora richardson larvae and juvenile microcapsule feed, as well as processing preparation and feeding method thereof
CN107027664B (en) Method for breeding micropterus salmoides
CN103549118B (en) Low salinity stocking Environment of Litopenaeus vannamei Low efficient and environment-friendly type mixed feed and preparation method thereof
Sontakke et al. Economic viability of biofloc based system for the nursery rearing of milkfish (Chanos chanos)
CN110731417B (en) Microbial fermentation feed for crayfish and preparation method thereof
CN104719222A (en) Tor brevifilis, grass carp, chub and freshwater shrimp mixed aquaculture method
CN106387444A (en) Cat food and preparation method thereof
CN102396653A (en) Granulated feed for procypris rabaudi
CN102742739A (en) Feed formula for odontobutis obscurus
CN105130679A (en) Efficient large-water-surface fishing bacteria-algae balancing bio-fertilizer
CN103734490B (en) A kind of Crab Portunus trituberculatus Larvae cultivates feed
CN104026417B (en) A kind of mandarin fish culturing feed
Lagutkina et al. Biotech aspects of Caridean shrimp cultivation
CN103503807B (en) Method for cultivating coho salmon seedlings
CN104798995A (en) Saladfish young fish feed
CN104187163A (en) Feed capable of increasing laying rate of truogx sinensis and production method of feed
CN101904454B (en) Bait for promoting mature of genital glands of parent shrimps of white shrimps in South America and preparation method thereof
CN101273760A (en) Black rockfish phagostimulant
CN105533265A (en) Pet-crab feed of easy digestion and easy absorption as well as production method thereof
CN108813209A (en) A kind of little yellow croaker young stage feed and preparation method thereof
CN102485020B (en) Soft feed for babylonia areolata parent mature stage and preparation method thereof
CN104115800A (en) Grouper fry larval stage feed and preparing method thereof
CN101120728A (en) Fodder additives for ox
CN106942522A (en) A kind of spotted maigre juvenile fish vitamin premix and preparation method thereof

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant