CN102845653B - Nibea albiflora richardson larvae and juvenile microcapsule feed, as well as processing preparation and feeding method thereof - Google Patents

Nibea albiflora richardson larvae and juvenile microcapsule feed, as well as processing preparation and feeding method thereof Download PDF

Info

Publication number
CN102845653B
CN102845653B CN2012103893589A CN201210389358A CN102845653B CN 102845653 B CN102845653 B CN 102845653B CN 2012103893589 A CN2012103893589 A CN 2012103893589A CN 201210389358 A CN201210389358 A CN 201210389358A CN 102845653 B CN102845653 B CN 102845653B
Authority
CN
China
Prior art keywords
feed
micro
mixed
make
raw material
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN2012103893589A
Other languages
Chinese (zh)
Other versions
CN102845653A (en
Inventor
谢中国
楼宝
王芙蓉
史会来
徐冬冬
詹炜
毛国民
辛俭
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zhejiang Marine Fisheries Research Institute
Original Assignee
Zhejiang Marine Fisheries Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zhejiang Marine Fisheries Research Institute filed Critical Zhejiang Marine Fisheries Research Institute
Priority to CN2012103893589A priority Critical patent/CN102845653B/en
Publication of CN102845653A publication Critical patent/CN102845653A/en
Application granted granted Critical
Publication of CN102845653B publication Critical patent/CN102845653B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Landscapes

  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)

Abstract

The invention provides a nibea albiflora richardson larvae and juvenile microcapsule feed which is prepared from white fish meal, euphausiid meal, squid meal, fish protein hydrolysate, feed yeast, alpha-starch, soybean lecithin, papain, fish oil, composite vitamin, composite mineral substance, binder, immunopotentiator, phagostimulant and the like. By adopting the innovated prescription and processing process, the microcapsule feed can fulfill the requirement for nutrition of larvae and juvenile growth, has the advantages of comprehensive nutrition, good palatability, high water stability, low cost and the like, can be produced in batches, is convenient for storing, transporting and feeding, is suitable for industrial breeding production, and adopts a corresponding feeding method, so that the survival rate of nibea albiflora richardson is greatly improved, the shape of the nibea albiflora richardson is increase. The nibea albiflora richardson larvae and juvenile microcapsule feed is suitable for large-scale popularization and application.

Description

A kind of spotted maigre young postlarva micro-encapsulated diet and processing preparation and feeding method
Technical field
The invention belongs to agriculture mariculture technology, specifically a kind of spotted maigre young postlarva micro-encapsulated diet and processing preparation and feeding method.
Background technology
Spotted maigre ( nibea albiflorarichardson), be commonly called as yellow mother-in-law chicken, belong to Perciformes, Sciaenidae, Nibea, the bodily form is similar to large yellow croaker, belongs to lower water layer fish in warm warm nature coastal waters, mainly inhabits the more shallow littoral sea of sand mud substrate, the zoobenthos such as small-sized shell-fish and small fish of take are food, mainly be distributed in the northwest Pacific district, the South Sea of China, the East Sea and the Huanghai Sea all have its distribution, 8~33 ℃ of comfort zones, suitable salt scope 10~40 ‰, the most suitable growth water temperature is 24~29 ℃, 24~28 ℃ of the water temperatures of laying eggs, and age at sexual maturity is 2 week age.Spotted maigre is the important marine fish of China, has meat flavour deliciousness, nutritious, culture benefit high.At present, the extensive artificial breeding of having carried out spotted maigre in Fujian and the Zhejiang area of China, but the special compound feed of spotted maigre young postlarva is is not yet researched and developed, cultivate feed and mainly adopt wheel animalcule, halogen worm and Copepods etc., adopt existing marine fish larvae feedstuff feeding spotted maigre, there are the series of problems such as hypotrophy face, palatability is poor, absorption rate is low, had a strong impact on the survival rate of spotted maigre, restricted further developing of its aquaculture industry.Therefore, carry out the development of the special-purpose micro-encapsulated diet of spotted maigre young postlarva, raising spotted maigre weed survival rate, solution spotted maigre propagation in scale, quickening spotted maigre aquaculture industry process are all had to positive facilitation.
Summary of the invention
The problems and shortcomings that exist for the existing feed of spotted maigre young postlarva, the present invention is according to the characteristics such as nutritional need, eating habit and digestive physiology of spotted maigre young postlarva, adopt methods such as optimizing feed formula, interpolation immunopotentiator and phagostimulant, be prepared into the micro-encapsulated diet of spotted maigre young postlarva special use by technological processes such as ultramicro grinding, mixing, granulation, dressings, and adopted corresponding feeding method to be thrown something and fed.
A kind of spotted maigre young postlarva micro-encapsulated diet, is characterized in that its raw material by following weight percentage makes: white fish meal 30%-50%, krill meal 10%-20%, squid powder 5%-15%, fish protein hydrolysate levels 2%-8%, fodder yeast 3%-9%, alphalise starch 7.5%-8.5%, soybean lecithin 2%-8%, papain 1%-3%, fish oil 1%-5%, B B-complex 1%-3%, composite mineral matter 1%-3%, binding agent 0.5%-1.5%, immunopotentiator 0.05%-0.15%, phagostimulant 0.5%-1.5%.
Described a kind of spotted maigre young postlarva micro-encapsulated diet, is characterized in that its raw material by following weight percentage makes: white fish meal 35%-45%, krill meal 12%-18%, squid powder 8%-12%, fish protein hydrolysate levels 4%-6%, fodder yeast 5%-7%, alphalise starch 7.8%-8%, soybean lecithin 4%-6%, papain 1.5%-2.5%, fish oil 2%-4%, B B-complex 1.5%-2.5%, composite mineral matter 1.5%-2.5%, binding agent 0.8%-1.2%, immunopotentiator 0.08%-0.12%, phagostimulant 0.8%-1.2%.
Described a kind of spotted maigre young postlarva micro-encapsulated diet; it is characterized in that its raw material by following weight percentage makes: white fish meal 40%, krill meal 15%, squid powder 10%, fish protein hydrolysate levels 5%, fodder yeast 6%, alphalise starch 7.9%, soybean lecithin 5%, papain 2%, fish oil 3%, B B-complex 2%, composite mineral matter 2%, binding agent 1%, immunopotentiator 0.1%, phagostimulant 1%; wherein said binding agent is sodium alginate; described immunopotentiator is glucan and shitosan, and described phagostimulant is taurine.
The preparation method of described a kind of spotted maigre young postlarva micro-encapsulated diet, is characterized in that comprising the steps:
1) the solid feed raw material is pulverized with micronizer;
2) all feeds raw material is weighed, mixed in the ratio of feed formula, first fully mix by hand, by 100 eye mesh screens, then mixed with mixer;
3) mixed raw material is made to particulate;
4) will at the bottom of the particulate feed employing fluid bed of making, spray or carry out dressing with sugar coating machine, coating solution is uniformly sprayed on the feed surface, make micro-encapsulated diet, wherein coating solution adopts a kind of in the ethanolic solution of ethanolic solution, resin of ethanolic solution, the zeins of the aqueous solution, the ethyl cellulose of gelatin;
5) by the preparation micro-encapsulated diet by the shaking screen of different meshes, carry out grain size grading with sieve method;
6) by classification, good feed is sealed in aluminium foil bag with vacuum packing machine, is stored in the refrigerator of 4 ℃ stand-by.
Described preparation method, it is characterized in that adopting extrusion-spheronization to make particulate mixed raw material in step 3), comprise the steps: mixed material is added the water of core amount 5%-10%, with trough type mixing machine with rotating speed 20-30rpm, the process conditions that the batch turning angle is 100-120 ° are mixed 10-30min, make the uniform moist wood of moisture content, the moist wood prepared is placed in to the stainless steel screen drum of rotating type granulating machine, through rotary squeezing, form cylindrical bars shape feed by mesh, and the method that is aided with hand-crushed becomes wet granular, particle diameter is controlled at 150~600 μ m, wet granular is put into and thrown circle 20-40min with rotating speed 5-15rpm in the rotating centrifugal dish of shot-blasting machine and make the ball ball.
Described preparation method, it is characterized in that comprising the steps: mixed material is added the water of core amount 8%, with trough type mixing machine with rotating speed 24rpm, the process conditions that the batch turning angle is 105 ° are mixed 20min, make the uniform moist wood of moisture content, the moist wood prepared is put in the stainless steel screen drum of rotating type granulating machine, through rotary squeezing, form cylindrical bars shape feed by 50 purpose mesh, and the method that is aided with hand-crushed becomes wet granular, particle diameter is controlled at 150~600 μ m, wet granular is put into and thrown circle 30min with rotating speed 10rpm in the rotating centrifugal dish of shot-blasting machine and make the ball ball.
Described preparation method; it is characterized in that adopting the efficient wet granulator to make particulate mixed raw material in step 3); comprise the steps: mixed feed is put into to the efficient wet granulator; mesh by certain order number is made particulate, and the large I of particulate is regulated according to the size of mesh.
Described preparation method, it is characterized in that in step 3) mixed raw material being adopted fluid bed top spray method make particulate, comprise the steps: mixed material is placed in fluid bed, peristaltic pump sprays into water, flow is 1-5mL/min, the EAT of fluid bed is that 60-80 ℃, leaving air temp are 30-50 ℃, 40-60 ℃ of bed temperatures, compressed air pressure 20-30Mpa, and the feed product particle diameter made is distributed between 150~600 μ m; In step 4), adopt the method for spraying at the bottom of fluid bed to carry out dressing, with hot water soluble gelatin solution, carry out end spray, process conditions are that EAT is 50-70 ℃, leaving air temp is 20-40 ℃, bed temperature is 35-45 ℃, atmospheric pressure 15-25mPa, and the flow velocity of peristaltic pump is 0.5-1.5 mL/min.
Described preparation method, it is characterized in that in step 3) mixed raw material being adopted fluid bed top spray method make particulate, comprise the steps: mixed material is placed in fluid bed, peristaltic pump sprays into water, flow is 3mL/min, the EAT of fluid bed is that 70 ℃, leaving air temp are 40 ℃, 50 ℃ of bed temperatures, compressed air pressure 25Mpa, and the feed product particle diameter made is distributed between 150~600 μ m; In step 4), adopt the method for spraying at the bottom of fluid bed to carry out dressing, with hot water soluble gelatin solution, carry out end spray, process conditions are that EAT is 60 ℃, and leaving air temp is 30 ℃, and bed temperature is 40 ℃, atmospheric pressure 20mPa, and the flow velocity of peristaltic pump is 1mL/min.
The feeding method of described a kind of spotted maigre young postlarva micro-encapsulated diet, it is characterized in that comprising the steps: the spotted maigre fry is carried out the feed transition period of 5 days, within the transition period, reduce the consumption of 20% biological live bait every day, increase the consumption of 20% micro-encapsulated diet every day, throw something and feed during the 15-25 age in days micro-encapsulated diet of 150-250 μ m particle diameter, be replaced by the micro-encapsulated diet of 200-450 μ m particle diameter during the 25-45 age in days, 5.0-8.0 mg/ tail/sky, increase aeration quantity simultaneously, make Feed Energy be distributed in uniformly in water body, more than dissolved oxygen in nursery pond remains on 5mg/L, preferably more than 6.0mg/L, ammonia-nitrogen content is less than 0.02mg/L, content of nitrite is less than 0.01mg/L, keeping temperature of cultivation is 26 ± 2 ℃, salinity is 30 ± 1 ‰, the pH value is 8.2 ± 0.2, adopt on a small quantity repeatedly, the mode of being satiated with food and throwing something and feeding, throw something and feed every day 6 times, inhale every day at the bottom of pond 1 time, with the residual bait at the bottom of the removing pond and dead seedling, remove in time the dirt of floating on water, quantity of exchanged water is 80%.
A kind of spotted maigre young postlarva micro-encapsulated diet of the present invention, adopt formula and the processing technology of innovation, can meet the nutritional need of young postlarva growth, having again comprehensive nutrition, good palatability, water stability can wait advantage well, can manufacture, and is convenient to storage, transports and throw something and feed, the seed that is applicable to batch production produces, adopted corresponding feeding method simultaneously, can improve growth and the survival rate of spotted maigre young postlarva, be applicable to large-scale promotion and use.
The specific embodiment
In April, 2012, in Southern Yangtze University's Foodstuffs Academy technological experiment chamber, prepared micro-encapsulated diet.5~June in 2012, dodge test site in Zhejiang Prov. Inst. of Marine Products west and carried out the culture efficiency evaluation test of spotted maigre young postlarva micro-encapsulated diet.The Gunther parent fish spawning that the young postlarva of spotted maigre is cultivated from test site, hatching gained.The incubation temperature of ovum is 26 ± 1 ℃, and the fry hatched is supported temporarily in indoor cement pit, and between nursery stage, water temperature is 26 ± 1 ℃, and salinity is 26~28 ‰.From young postlarva hatching, feed through wheel animalcule, the halogen worm of fortification, until on-test.
Embodiment 1
One, feed formula
The consisting of of feed formula (take 1000g): (crude protein is 67% to white fish meal; Crude fat 9% (dry weight), U.S. seafood company), 400g; (crude protein is 63.9% to krill meal; Crude fat is 5.6% (dry weight), Zhoushan Fengshun fish meal Products Co., Ltd), 150g; Fish protein hydrolysate levels (crude protein is 64.3%, and crude fat is 7.6% (dry weight), and self-control adopts trypsase and papain hydrolysis white fish meal to make) 50g; (crude protein is 50.3% to squid powder; Crude fat is 4.9% (dry weight), Zhoushan Marine Fishery Co., State Bureau of Aquatic Products's fish meal factory), 100g; Fodder yeast, 60g; Alphalise starch, 79g; Soybean lecithin, 50g; Papain, 20g; Sodium alginate (binding agent), 10g; Fish oil, 30g; B B-complex, 20g; Composite mineral matter, 20g, taurine (phagostimulant and immunopotentiator), 10g; Glucan (immunopotentiator), 0.5g; Shitosan (immunopotentiator), 0.5g.
The feed nutrition of making consists of: moisture, 7.32%; Crude protein, 56.47%; Crude fat, 16.15%; Crude fibre: 2.76%; Coarse ash, 13.86%; Salt, 1.63%; Calcium, 2.47%; Total phosphorus, 1.31%; Lysine, 3.43%.
Two, the preparation method of micro-encapsulated diet
Solid material and micronizer for additive (WFJ series micronizer, Jiangyin City size reduction machinery Co., Ltd of gold section) are pulverized, and granularity is less than 20 μ m.The all feeds raw material is weighed in proportion, the principle of stack is mixed in a small amount, add a certain proportion of water, first fully mix by hand, all by 100 mesh sieves, then use 3 D multi-directional movement mixer (YH series 3 D multi-directional movement mixer, Changzhou Pu Naier drying equipment Co., Ltd) to mix 10 min.By the method granulation of fluid bed top spray, process conditions are that EAT is 70 ℃, and leaving air temp is 40 ℃, and bed temperature is 50 ℃, atmospheric pressure 25mPa, and the flow velocity of peristaltic pump is 1 mL/min, pumps into water, the time is 60min.Then use spray method at the bottom of fluid bed instead, hot water soluble gelatin solution is carried out to end spray, process conditions are that EAT is 60 ℃, leaving air temp is 30 ℃, and bed temperature is 40 ℃, atmospheric pressure 20mPa, the flow velocity of peristaltic pump is 1 mL/min, single crowd of output 2kg, coating material is gelatin solution, the consumption of gelatin is 100g.
By 100 orders, 80 orders, 60 orders, 40 orders, 30 orders, 20 orders, 10 purpose shaking screens, adopt sieve method to carry out grain size grading the micro-encapsulated diet of preparation.By classification, good feed is with in vacuum packing machine sealing and aluminium foil bag, is stored in the refrigerator of 4 ℃ stand-by.
Three, culture experiment
Using the spotted maigre prelarva of 15 ages in days as test material, and total length is 0.5cm.The spotted maigre prelarva is moved into to 2 2.5m 3the cement culturing pool in, 20,000 tails are put in every pond.The Copepods of control group fed refrigeration.Test group, through feed transition period of 5 days, progressively reduces the consumption (reducing 20% every day) of halogen worm within the transition period, progressively increases the consumption (increasing by 20% every day) of micro-encapsulated diet (control group Copepods) simultaneously.According to the size of young postlarva and the bore of ingesting, the consumption of the particle diameter of micro-encapsulated diet and consumption and Copepods increases along with the growth of young postlarva.Throw something and feed during the 15-25 age in days micro-encapsulated diet (5.0-8.0 mg/ tail/sky) of 150-250 μ m particle diameter, be replaced by 200-450 μ m particle diameter during the 25-45 age in days.Control group 15-25 age in days fry is thrown something and fed and refrigerates Copepods (10.0-15.0mg/ tail/sky).Accomplish agreeable to the taste, appropriate, in good time.It is that 26 ± 2 ℃, salinity are 30 ± 1 ‰, the pH value is 8.2 ± 0.2 that duration of test keeps temperature of cultivation.More than dissolved oxygen is controlled at 6.0mg/L, ammonia-nitrogen content is less than 0.02mg/L, and content of nitrite is less than 0.01mg/L.Inhale every day at the bottom of pond 1 time, to remove residual bait and the dead seedling at the bottom of pond; Remove in time the dirt of floating on water, quantity of exchanged water is 80%.Cultivation is carried out 30 days, and the survival rate of the spotted maigre of test group is 32.6%, and the survival rate of control group is 21.3%; The average length of test group spotted maigre is 4.2cm, and the average length of control group spotted maigre is 3.6cm.
Embodiment 2
One, feed formula
The consisting of of feed formula (take 1000g): (crude protein is 67% to white fish meal; Crude fat 9% (dry weight), U.S. seafood company), 350g; (crude protein is 63.9% to krill meal; Crude fat is 5.6% (dry weight), Zhoushan Fengshun fish meal Products Co., Ltd), 180g; Fish protein hydrolysate levels (crude protein is 64.3%, and crude fat is 7.6% (dry weight), and self-control adopts trypsase and papain hydrolysis white fish meal to make) 40g; (crude protein is 50.3% to squid powder; Crude fat is 4.9% (dry weight), Zhoushan Marine Fishery Co., State Bureau of Aquatic Products's fish meal factory), 120g; Fodder yeast, 70g; Alphalise starch, 78g; Soybean lecithin, 40g; Papain, 25g; Sodium alginate (binding agent), 12g; Fish oil, 22g; B B-complex, 25g; Composite mineral matter, 25g, taurine (phagostimulant and immunopotentiator), 12g; Glucan (immunopotentiator), 0.5g; Shitosan (immunopotentiator), 0.5g.
The feed nutrition of making consists of: moisture, 7.68%; Crude protein, 56.11%; Crude fat, 16.25%; Crude fibre: 2.66%; Coarse ash, 13.86%; Salt, 1.63%; Calcium, 2.57%; Total phosphorus, 1.38%; Lysine, 3.49%.
Two, the preparation method of micro-encapsulated diet
Solid material and micronizer for additive (WFJ series micronizer, Jiangyin City size reduction machinery Co., Ltd of gold section) are pulverized, and granularity is less than 20 μ m.The all feeds raw material is weighed in proportion, the principle of stack is mixed in a small amount, add a certain proportion of water, first fully mix by hand, all by 100 mesh sieves, then use 3 D multi-directional movement mixer (YH series 3 D multi-directional movement mixer, Changzhou Pu Naier drying equipment Co., Ltd) to mix 10 min.Method granulation by extrusion-spheronization; the water that mixed material is added to core amount 8%; with trough type mixing machine with rotating speed 24rpm; the process conditions that the batch turning angle is 105 ° are mixed 20min; make the uniform moist wood of moisture content; the moist wood prepared is put in the stainless steel screen drum of rotating type granulating machine; through rotary squeezing; form cylindrical bars shape feed by 50 purpose mesh; and the method that is aided with hand-crushed becomes wet granular; particle diameter is controlled at 150~600 μ m, wet granular is put into throw circle 30min with rotating speed 10rpm in the rotating centrifugal dish of shot-blasting machine and make the ball ball.Then use spray method at the bottom of fluid bed instead, hot water soluble gelatin solution is carried out to end spray, process conditions are that EAT is 50 ℃, leaving air temp is 20 ℃, and bed temperature is 35 ℃, atmospheric pressure 15mPa, the flow velocity of peristaltic pump is 0.5 mL/min, single crowd of output 2kg, coating material is gelatin solution, the consumption of gelatin is 100g.
By 100 orders, 80 orders, 60 orders, 40 orders, 30 orders, 20 orders, 10 purpose shaking screens, adopt sieve method to carry out grain size grading the micro-encapsulated diet of preparation.By classification, good feed is with in vacuum packing machine sealing and aluminium foil bag, is stored in the refrigerator of 4 ℃ stand-by.
Three, culture experiment
Using the spotted maigre prelarva of 15 ages in days as test material, and total length is 0.5cm.The spotted maigre prelarva is moved into to 2 2.5m 3the cement culturing pool in, 20,000 tails are put in every pond.The Copepods of control group fed refrigeration.Test group, through feed transition period of 5 days, progressively reduces the consumption (reducing 20% every day) of halogen worm within the transition period, progressively increases the consumption (increasing by 20% every day) of micro-encapsulated diet (control group Copepods) simultaneously.According to the size of young postlarva and the bore of ingesting, the consumption of the particle diameter of micro-encapsulated diet and consumption and Copepods increases along with the growth of young postlarva.Throw something and feed during the 15-25 age in days micro-encapsulated diet (5.0-8.0 mg/ tail/sky) of 150-250 μ m particle diameter, be replaced by 200-450 μ m particle diameter during the 25-45 age in days.Control group 15-25 age in days fry is thrown something and fed and refrigerates Copepods (10.0-15.0mg/ tail/sky).Accomplish agreeable to the taste, appropriate, in good time.It is that 26 ± 2 ℃, salinity are 30 ± 1 ‰, the pH value is 8.2 ± 0.2 that duration of test keeps temperature of cultivation.More than dissolved oxygen is controlled at 5.0mg/L, ammonia-nitrogen content is less than 0.02mg/L, and content of nitrite is less than 0.01mg/L.Inhale every day at the bottom of pond 1 time, to remove residual bait and the dead seedling at the bottom of pond; Remove in time the dirt of floating on water, quantity of exchanged water is 80%.Cultivation is carried out 30 days, and the survival rate of the spotted maigre of test group is 30.5%, and the survival rate of control group is 21.1%; The average length of test group spotted maigre is 4.0cm, and the average length of control group spotted maigre is 3.7cm.
Embodiment 3
One, feed formula
The consisting of of feed formula (take 1000g): (crude protein is 67% to white fish meal; Crude fat 9% (dry weight), U.S. seafood company), 450g; (crude protein is 63.9% to krill meal; Crude fat is 5.6% (dry weight), Zhoushan Fengshun fish meal Products Co., Ltd), 120g; Fish protein hydrolysate levels (crude protein is 64.3%, and crude fat is 7.6% (dry weight), and self-control adopts trypsase and papain hydrolysis white fish meal to make) 60g; (crude protein is 50.3% to squid powder; Crude fat is 4.9% (dry weight), Zhoushan Marine Fishery Co., State Bureau of Aquatic Products's fish meal factory), 80g; Fodder yeast, 50g; Alphalise starch, 80g; Soybean lecithin, 60g; Papain, 15g; Sodium alginate (binding agent), 8g; Fish oil, 38g; B B-complex, 15g; Composite mineral matter, 15g, taurine (phagostimulant and immunopotentiator), 8g; Glucan (immunopotentiator), 0.5g; Shitosan (immunopotentiator), 0.5g.
The feed nutrition of making consists of: moisture, 7.52%; Crude protein, 56.25%; Crude fat, 16.1%; Crude fibre: 2.44%; Coarse ash, 13.95%; Salt, 1.89%; Calcium, 2.47%; Total phosphorus, 1.95%; Lysine, 3.74%.
Two, the preparation method of micro-encapsulated diet
Solid material and micronizer for additive (WFJ series micronizer, Jiangyin City size reduction machinery Co., Ltd of gold section) are pulverized, and granularity is less than 20 μ m.The all feeds raw material is weighed in proportion, the principle of stack is mixed in a small amount, add a certain proportion of water, first fully mix by hand, all by 100 mesh sieves, then use 3 D multi-directional movement mixer (YH series 3 D multi-directional movement mixer, Changzhou Pu Naier drying equipment Co., Ltd) to mix 10 min.Adopt the efficient wet granulator to make particulate, mixed feed is put into to the efficient wet granulator, by 50 purpose meshes, make particulate.Then use spray method at the bottom of fluid bed instead, hot water soluble gelatin solution is carried out to end spray, process conditions are that EAT is 70 ℃, leaving air temp is 40 ℃, and bed temperature is 45 ℃, atmospheric pressure 25mPa, the flow velocity of peristaltic pump is 1.5 mL/min, single crowd of output 2kg, coating material is gelatin solution, the consumption of gelatin is 100g.
By 100 orders, 80 orders, 60 orders, 40 orders, 30 orders, 20 orders, 10 purpose shaking screens, adopt sieve method to carry out grain size grading the micro-encapsulated diet of preparation.By classification, good feed is with in vacuum packing machine sealing and aluminium foil bag, is stored in the refrigerator of 4 ℃ stand-by.
Three, culture experiment
Using the spotted maigre prelarva of 15 ages in days as test material, and total length is 0.5cm.The spotted maigre prelarva is moved into to 2 2.5m 3the cement culturing pool in, 20,000 tails are put in every pond.The Copepods of control group fed refrigeration.Test group, through feed transition period of 5 days, progressively reduces the consumption (reducing 20% every day) of halogen worm within the transition period, progressively increases the consumption (increasing by 20% every day) of micro-encapsulated diet (control group Copepods) simultaneously.According to the size of young postlarva and the bore of ingesting, the consumption of the particle diameter of micro-encapsulated diet and consumption and Copepods increases along with the growth of young postlarva.Throw something and feed during the 15-25 age in days micro-encapsulated diet (5.0-8.0 mg/ tail/sky) of 150-250 μ m particle diameter, be replaced by 200-450 μ m particle diameter during the 25-45 age in days.Control group 15-25 age in days fry is thrown something and fed and refrigerates Copepods (10.0-15.0mg/ tail/sky).Accomplish agreeable to the taste, appropriate, in good time.It is that 26 ± 2 ℃, salinity are 30 ± 1 ‰, the pH value is 8.2 ± 0.2 that duration of test keeps temperature of cultivation.More than dissolved oxygen is controlled at 6.0mg/L, ammonia-nitrogen content is less than 0.02mg/L, and content of nitrite is less than 0.01mg/L.Inhale every day at the bottom of pond 1 time, to remove residual bait and the dead seedling at the bottom of pond; Remove in time the dirt of floating on water, quantity of exchanged water is 80%.Cultivation is carried out 30 days, and the survival rate of the spotted maigre of test group is 29.9%, and the survival rate of control group is 21.6%; The average length of test group spotted maigre is 3.9cm, and the average length of control group spotted maigre is 3.5cm.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any modifications of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all being included in protection scope of the present invention.

Claims (5)

1. a spotted maigre young postlarva micro-encapsulated diet, it is characterized in that its raw material by following weight percentage makes: white fish meal 35%-45%, krill meal 12%-18%, squid powder 8%-12%, fish protein hydrolysate levels 4%-6%, fodder yeast 5%-7%, alphalise starch 7.8%-8%, soybean lecithin 4%-6%, papain 1.5%-2.5%, fish oil 2%-4%, B B-complex 1.5%-2.5%, composite mineral matter 1.5%-2.5%, binding agent 0.8%-1.2%, immunopotentiator 0.08%-0.12%, phagostimulant 0.8%-1.2%, wherein said binding agent is sodium alginate, described immunopotentiator is glucan and shitosan, described phagostimulant is taurine, and adopt the preparation method's preparation comprised the steps:
1) the solid feed raw material is pulverized with micronizer;
2) all feeds raw material is weighed, mixed in the ratio of feed formula, first fully mix by hand, by 100 eye mesh screens, then mixed with mixer;
3) mixed raw material is made to particulate;
4) by the capable dressing of spirt at the bottom of the particulate feed employing fluid bed of making, coating solution is uniformly sprayed on the feed surface, make micro-encapsulated diet, wherein coating solution adopts the aqueous solution of gelatin;
5) by the preparation micro-encapsulated diet by the shaking screen of different meshes, carry out grain size grading with sieve method;
6) by classification, good feed is sealed in aluminium foil bag with vacuum packing machine, is stored in the refrigerator of 4 ℃ stand-by;
Wherein in step 3), adopt one of following 3 kinds of methods to make particulate:
A. mixed raw material is adopted fluid bed top spray method to make particulate, comprise the steps: mixed material is placed in fluid bed, peristaltic pump sprays into water, flow is 0.5-1.5mL/min, the EAT of fluid bed is that 60-80 ℃, leaving air temp are 30-50 ℃, 40-60 ℃ of bed temperatures, compressed air pressure 20-30MPa, and the feed product particle diameter made is distributed between 150~600 μ m;
B. adopt extrusion-spheronization to make particulate mixed raw material, comprise the steps: mixed material is added the water of core amount 5%-10%, with trough type mixing machine with rotating speed 20-30rpm, the process conditions that the batch turning angle is 100-120 ° are mixed 10-30min, make the uniform moist wood of moisture content, the moist wood prepared is placed in to the stainless steel screen drum of rotating type granulating machine, through rotary squeezing, form cylindrical bars shape feed by mesh, and the method that is aided with hand-crushed becomes wet granular, particle diameter is controlled at 150~600 μ m, wet granular is put into and thrown circle 20-40min with rotating speed 5-15rpm in the rotating centrifugal dish of shot-blasting machine and make the ball ball,
C. adopt the efficient wet granulator to make particulate mixed raw material, comprise the steps: mixed feed is put into to the efficient wet granulator, by the mesh of certain order number, make particulate, the size of particulate is regulated according to the size of mesh;
In step 4), adopt the method for spraying at the bottom of fluid bed to carry out dressing, with hot water soluble gelatin solution, carry out end spray, process conditions are that EAT is 50-70 ℃, leaving air temp is 20-40 ℃, bed temperature is 35-45 ℃, atmospheric pressure 15-25MPa, and the flow velocity of peristaltic pump is 0.5-1.5 mL/min.
2. a kind of spotted maigre young postlarva micro-encapsulated diet as claimed in claim 1, is characterized in that its raw material by following weight percentage makes: white fish meal 40%, krill meal 15%, squid powder 10%, fish protein hydrolysate levels 5%, fodder yeast 6%, alphalise starch 7.9%, soybean lecithin 5%, papain 2%, fish oil 3%, B B-complex 2%, composite mineral matter 2%, binding agent 1%, immunopotentiator 0.1%, phagostimulant 1%.
3. a kind of spotted maigre young postlarva micro-encapsulated diet as claimed in claim 1, it is characterized in that b method in step 3) comprises the steps: mixed material is added the water of core amount 8%, with trough type mixing machine with rotating speed 24rpm, the process conditions that the batch turning angle is 105 ° are mixed 20min, make the uniform moist wood of moisture content, the moist wood prepared is placed in to the stainless steel screen drum of rotating type granulating machine, through rotary squeezing, form cylindrical bars shape feed by 50 purpose mesh, and the method that is aided with hand-crushed becomes wet granular, particle diameter is controlled at 150~600 μ m, wet granular is put into and thrown circle 30min with rotating speed 10rpm in the rotating centrifugal dish of shot-blasting machine and make the ball ball.
4. a kind of spotted maigre young postlarva micro-encapsulated diet as claimed in claim 1, it is characterized in that a method in step 3) draws together following steps: mixed material is placed in fluid bed, peristaltic pump sprays into water, flow is 1mL/min, the EAT of fluid bed is that 70 ℃, leaving air temp are 40 ℃, 50 ℃ of bed temperatures, compressed air pressure 25MPa, and the feed product particle diameter made is distributed between 150~600 μ m; In step 4), adopt the method for spraying at the bottom of fluid bed to carry out dressing, with hot water soluble gelatin solution, carry out end spray, process conditions are that EAT is 60 ℃, and leaving air temp is 30 ℃, and bed temperature is 40 ℃, atmospheric pressure 20MPa, and the flow velocity of peristaltic pump is 1mL/min.
5. the feeding method of a kind of spotted maigre young postlarva micro-encapsulated diet as described as any one in claim 1 to 4, it is characterized in that comprising the steps: the spotted maigre fry is carried out the feed transition period of 5 days, within the transition period, reduce the consumption of 20% biological live bait every day, increase the consumption of 20% micro-encapsulated diet every day, throw something and feed during the 15-25 age in days micro-encapsulated diet of 150-250 μ m particle diameter, be replaced by the micro-encapsulated diet of 200-450 μ m particle diameter during the 25-45 age in days, 5.0-8.0 mg/ tail/sky, increase aeration quantity simultaneously, make Feed Energy be distributed in uniformly in water body, more than dissolved oxygen in nursery pond remains on 5mg/L, ammonia-nitrogen content is less than 0.02mg/L, content of nitrite is less than 0.01mg/L, keeping temperature of cultivation is 26 ± 2 ℃, salinity is 30 ± 1 ‰, the pH value is 8.2 ± 0.2, adopt on a small quantity repeatedly, the mode of being satiated with food and throwing something and feeding, throw something and feed every day 6 times, inhale every day at the bottom of pond 1 time, with the residual bait at the bottom of the removing pond and dead seedling, remove in time the dirt of floating on water, quantity of exchanged water is 80%.
CN2012103893589A 2012-10-15 2012-10-15 Nibea albiflora richardson larvae and juvenile microcapsule feed, as well as processing preparation and feeding method thereof Active CN102845653B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN2012103893589A CN102845653B (en) 2012-10-15 2012-10-15 Nibea albiflora richardson larvae and juvenile microcapsule feed, as well as processing preparation and feeding method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN2012103893589A CN102845653B (en) 2012-10-15 2012-10-15 Nibea albiflora richardson larvae and juvenile microcapsule feed, as well as processing preparation and feeding method thereof

Publications (2)

Publication Number Publication Date
CN102845653A CN102845653A (en) 2013-01-02
CN102845653B true CN102845653B (en) 2013-12-18

Family

ID=47392950

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2012103893589A Active CN102845653B (en) 2012-10-15 2012-10-15 Nibea albiflora richardson larvae and juvenile microcapsule feed, as well as processing preparation and feeding method thereof

Country Status (1)

Country Link
CN (1) CN102845653B (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103392924A (en) * 2013-08-13 2013-11-20 江苏天石生物科技有限公司 Coated feed additive added with complex enzyme preparation
CN103652495B (en) * 2013-12-16 2015-06-17 中国科学院海洋研究所 Megalobatrachusdavidianus pellet feed and preparation method thereof
CN104758315B (en) * 2014-01-06 2019-05-31 香港理工大学 A method of fish oil is wrapped up using zeins
CN104000009B (en) * 2014-02-24 2016-02-17 舟山海洋科技有限公司 A kind of spotted maigre feed and preparation method thereof
WO2016033942A1 (en) * 2014-09-02 2016-03-10 广东英锐生物科技有限公司 Capsule feed and preparation method and use thereof
CN106689652A (en) * 2015-07-15 2017-05-24 雷遨宇 Pelagic high protein powder of bighead carp and preparation method of pelagic high protein powder
CN105028907B (en) * 2015-09-16 2019-01-29 湖南文理学院 A kind of Pelteobagrus fulvidraco larva and juvenile Intelligent controlled release micro-encapsulated diet and preparation method thereof
CN105410466A (en) * 2015-12-04 2016-03-23 上海农好饲料有限公司 Functional feed used for inhibiting growth of gonads of Nibea albifloras and preparation method thereof
CN106036208B (en) * 2016-06-01 2019-10-01 广东德宁水产科技有限公司 A kind of aquatic product sprout feed and its production method
CN105876163A (en) * 2016-06-28 2016-08-24 张家银 Loach coated feed capable of realizing soybean meal fermentation modification by sophora japonica polysaccharides and improving delicious degree of fish and preparation method for loach coated feed
CN106387352A (en) * 2016-11-29 2017-02-15 南宁学院 Microcapsule animal immunoenhancer containing tea polyphenol and preparation method of immunoenhancer
CN108094693A (en) * 2017-12-29 2018-06-01 福建省淡水水产研究所 A kind of grouper larvae micro-encapsulated diet and preparation method thereof
CN108077651A (en) * 2017-12-31 2018-05-29 长沙善道新材料科技有限公司 A kind of spotted maigre young postlarva micro-encapsulated diet and preparation method thereof
CN108782367A (en) * 2018-06-26 2018-11-13 珠海容川饲料有限公司 The method and feed and preparation method and application of raising pseudosciaena crocea larvae survival rate
CN109329588B (en) * 2018-10-24 2022-02-08 浙江海洋大学 Compound feed for marine fish for deep-water cage culture
CN110393162A (en) * 2019-08-12 2019-11-01 汕头大学 A kind of cultural method for reducing Nibea coibor feed coefficient and improving air bladder quality
CN113694086A (en) * 2020-05-20 2021-11-26 香港理工大学深圳研究院 Probiotics capsule and preparation method thereof

Also Published As

Publication number Publication date
CN102845653A (en) 2013-01-02

Similar Documents

Publication Publication Date Title
CN102845653B (en) Nibea albiflora richardson larvae and juvenile microcapsule feed, as well as processing preparation and feeding method thereof
CN102934751B (en) Microcapsule bait, bait feeding method and bait feeding device for grouper larvae
CN104799124B (en) A kind of colored clam mixed feed
CN101129168B (en) Granulated feed of kirschner crawfish
CN103120269B (en) Pike overwintering feed and production method thereof
CN103976188B (en) A kind of yellow-head catfish feed and application thereof containing stratiomyiid worm powder
CN103844140B (en) Mixed bostrichthys sinensis feed and preparation method thereof
CN102835548B (en) Larval macrobrachium rosenbergii micro-pellet feed and preparation method thereof
CN105942025A (en) Nutritional feed promoting growth of eleutheronema tetradactylum as well as preparation and use methods of nutritional feed
CN103082150B (en) Reeves shad parent fish nutrition reinforcement forage and its preparation method
CN104543565B (en) A kind of spotted maigre compound feed for young and preparation method thereof
CN107744053A (en) A kind of Parent Female of Macrobrachium rosenbergii resisting stress promotees breeding pellet and preparation method thereof
CN108201025A (en) A kind of multifilament cray feed and preparation method thereof
CN102960556B (en) Young oplopanax elatus feed mainly prepared from enteromorpha and preparation method and feeding method thereof
CN104957430A (en) Epinephelus akaara compound feed and preparation method thereof
CN102934750B (en) Microcapsule bait feed for larval and juvenile oplegnathus fasciatus and feeding method
CN106721655A (en) Catfish scrod feed and its manufacture method
CN104206829B (en) A kind of monocycle thorn feedstuff and preparation method thereof
CN102934749B (en) Micro-capsule bait of tawny puffer larvae and juveniles and bait feeding method
CN101869201A (en) Snakehead feed
CN108077651A (en) A kind of spotted maigre young postlarva micro-encapsulated diet and preparation method thereof
CN103621790A (en) Composite earthworm enzymatic hydrolysate feed additive and preparation method thereof
CN103704489A (en) Special feed for intercropping yellow-head catfish and erythroculter ilishaeformis
CN104431418A (en) Stichopus japonicus complete formula feed
CN108902459A (en) A kind of breeding soft-shell turtle feed

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant