CN108850734A - It is a kind of improve Nibea coibor air bladder quality artifical compound feed and its preparation - Google Patents
It is a kind of improve Nibea coibor air bladder quality artifical compound feed and its preparation Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
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Abstract
The present invention relates to a kind of artifical compound feeds for improving Nibea coibor air bladder quality, including fish meal, fish oil, tapioca and auxiliary material, further include the hydroxyproline that weight percentage is 0.25~1.25%；The auxiliary material is selected from the group being made of choline chloride, microcrystalline cellulose, multi-vitamins, composite mineral matter, calcium dihydrogen phosphate, glutamic acid, kilnitamin and their one or more of mixtures；Wherein, the feed is made of the nutritional ingredient of following weight percent：Crude protein 42.78~42.96, crude fat 8.47~8.59, remainder are carbohydrate, crude fibre, ash content and moisture.The hydroxyproline that the present invention passes through the addition 0.25~1.25% in the artifical compound feed of Nibea coibor, and the component and its content of the other raw materials of Reasonable Regulation And Control and auxiliary material, Nibea coibor can be promoted to grow, increase the content of the necessary amino acid of crude protein content and methionine and lysine etc. in air bladder, also increase soluble collagen and total collagen content, to improve Nibea coibor air bladder quality.
Description
Technical field
The present invention relates to fish feed field more particularly to a kind of hand fit's feedings for improving Nibea coibor air bladder quality
Material.
Background technique
Air bladder also known as fish bubble, fish maw, the fish sperm, pressure born of the same parents, it is eponymous with bird's nest, shark's fin with famous rich in colloid, it is " ocean eight
One of it is precious ", and have the reputation of " ocean ginseng ".Collagen is most important ingredient in fish maw protein, since it is easily inhaled by body
The advantages of receiving and utilizing, can be used as protein needed for raw material carrys out synthesising biological body, collagen is in form of an aqueous solutions
It is stored in tissue, to achieve the purpose that improve histotrophic nutrition situation and accelerate body metabolism, can also influence
Certain specific organization's physiological functions promote growth, accelerate development, and strengthen immunity plays the aging for delaying body and resists disease
Effect of disease.The dry product of air bladder is that China coast production and processing is worth the edible treasure of very high aquatic products, and the country is additional at present
The major product form of work air bladder.In addition, air bladder economic value also with higher, adult fish is can be processed in its air bladder of the adult fish of 5kg
Glue weight about 50g~60g, the fish glue market price is 5000 yuan/kg~10000 yuan/kg, and fish glue product is in very great demand.
In numerous production fish glue ocean fishes, Nibea coibor (Nibea coibor), be commonly called as it is white how, spun gold catfish, byproduct-Bai Nai
Fish glue, i.e. " white flower glue " medicinal and nutritive value are only second to money catfish, and market value is very high.It also have growth is fast, individual is big,
Air bladder is big, it is disease-resistant it is strong etc. it is many a little and be suitable for China coast seawater cage culture, it is deep by the vast cage culture in the ground such as Fujian, Guangdong
The favor at family has good development prospect.However, existing into since Nibea coibor cultivation mainly feeds chilled wild fishes
The disadvantages of this height, seriously polluted breeding environment.Meanwhile iced fish nutrient structure is unbalanced, and the quality of air bladder is caused also to cannot get
Stablize and ensures.In terms of the existing economic value of Nibea coibor is mainly reflected in the flesh of fish, on the contrary in terms of being worth higher air bladder
Research is few.Therefore, the development of Nibea coibor aquaculture can be promoted by researching and developing one kind, and can improve Nibea coibor
The feed of air bladder quality is particularly necessary.
Summary of the invention
The purpose of the present invention is to provide a kind of artifical compound feeds for improving Nibea coibor air bladder quality, can promote
It is grown into Nibea coibor, improves Nibea coibor air bladder quality, it is of the existing technology to solve the problems, such as.
To solve the above problems, the present invention provides a kind of artifical compound feed for improving Nibea coibor air bladder quality,
Including fish meal, fish oil, tapioca and auxiliary material, which is characterized in that further include the hydroxyl that weight percentage is 0.25~1.25%
Proline；
The auxiliary material is selected from by choline chloride, microcrystalline cellulose, multi-vitamins, composite mineral matter, calcium dihydrogen phosphate, paddy
The group that propylhomoserin, kilnitamin and their one or more of mixtures are constituted；
Wherein, the feed is made of the nutritional ingredient of following weight percent：Crude protein 42.78~42.96, crude fat
8.47~8.59, remainder is carbohydrate, crude fibre, ash content and moisture.
Hydroxyproline (Hyp) is the product of Proline Metabolism, it contains only an alpha-imino, in chemical structure and biology
It learns in characteristic, there is unique function.Hydroxyproline is the primary amino acid in collagen, is constitute collagen main
Amino acid plays a significant role in the synthesis process of collagen, it is necessary to collagen three-dimensional spiral structure is maintained
, and the thermal stability of collagen Dai Bai can be enhanced and be able to suppress the generation of protein coagulation and fibrosis.In addition, hydroxyl
Proline is also considered as the necessary amino acid of condition of fish, and fish ingests after hydroxyproline content feedstuff abundant, can mention
Its high growth performance.Since Nibea coibor is predacious fish, 80% ingredient of air bladder is made of amino acid, and hydroxyproline is
The primary amino acid for constituting collagen, plays a significant role in the synthesis process of collagen.
Preferably, the weight percent of the hydroxyproline is 1%.
Preferably, the glutamic acid for being 0.25~1.25% including weight percentage.
It mainly include the raw material of following weight percentage as a kind of preferred embodiment of the invention：Fish meal 32~
36%, fish oil 5.6%, tapioca 20~24%, kilnitamin 17~19%, microcrystalline cellulose 13~15%, chlorination gallbladder
Alkali 1%, calcium dihydrogen phosphate 1%, glutamic acid 0.25~1.25%, multi-vitamins 1%, composite ore substance 1%, hydroxyproline
0.25~1.25%.
It mainly include the raw material of following weight percentage as more preferably a kind of embodiment of the invention：Fish meal
34.5%, fish oil 5.6%, tapioca 22.25%, kilnitamin 17.7%, microcrystalline cellulose 14.7%, choline chloride
1%, calcium dihydrogen phosphate 1%, glutamic acid 0.25%, multi-vitamins 1%, composite ore substance 1%, hydroxyproline 1%.
The preparation of the artifical compound feed of above-mentioned raising Nibea coibor air bladder quality mainly includes：It will be in addition to fish oil
Other raw materials and auxiliary material crush in advance and cross 80~120 meshes, and moisture is added after mixing, and extruding is pelletized after mixing, swelling temperature
It is 100~120 DEG C, finally fish oil is sprayed in feed, to get finished feed after fully absorbing.
The application of the artifical compound feed of above-mentioned raising Nibea coibor air bladder quality, for improving Nibea coibor air bladder
Quality.
Compared with prior art, the present invention in the artifical compound feed of Nibea coibor by adding 0.25~1.25%
Hydroxyproline, and the component and its content of the other raw materials of Reasonable Regulation And Control and auxiliary material can promote Nibea coibor to grow, and
Significantly improve Hydroxyproline in Sera and liver total hydroxyproline content, increase in air bladder crude protein content and methionine and
The content of the necessary amino acid such as lysine, also increases soluble collagen and total collagen content, to improve light color
Spotted maigre air bladder quality.The medical value and economic value for making Nibea coibor air bladder further increase, and mention for the production of air bladder
For certain theoretical direction.
Detailed description of the invention
Fig. 1 is that addition proline Nibea coibor air bladder total collagen (TC) content influences regression analysis；
Fig. 2 is the influence for adding proline to Nibea coibor juvenile fish air bladder, muscle, liver pH vigor；
Fig. 3 is the influence for adding proline to Nibea coibor juvenile fish serum representing prolyl hydroxylase enzyme activity.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, the present invention is made into one below in conjunction with attached drawing
Step ground detailed description.
Embodiment 1
A kind of artifical compound feed improving Nibea coibor air bladder quality, the raw material preparation including following weight percent
It forms：Fish meal 36%, fish oil 5.6%, tapioca 21%, kilnitamin 19%, microcrystalline cellulose 12.9%, choline chloride
1%, calcium dihydrogen phosphate 1%, glutamic acid 1.25%, multi-vitamins 1%, composite ore substance 1%, hydroxyproline 0.25%.
Embodiment 2
A kind of artifical compound feed improving Nibea coibor air bladder quality, the raw material preparation including following weight percent
It forms：Fish meal 34.5%, fish oil 5.6%, tapioca 22.25%, kilnitamin 17.7%, microcrystalline cellulose 14.7%,
Choline chloride 1%, calcium dihydrogen phosphate 1%, glutamic acid 0.75%, multi-vitamins 1%, composite ore substance 1%, hydroxyproline
0.5%.
Embodiment 3
A kind of artifical compound feed improving Nibea coibor air bladder quality, the raw material preparation including following weight percent
It forms：Fish meal 32%, fish oil 5.6%, tapioca 25%, kilnitamin 17%, microcrystalline cellulose 14.9%, choline chloride
1%, calcium dihydrogen phosphate 1%, glutamic acid 0.25%, multi-vitamins 1%, composite ore substance 1%, hydroxyproline 1.25%.
Embodiment 4
A kind of artifical compound feed improving Nibea coibor air bladder quality, the raw material preparation including following weight percent
It forms：Fish meal 34%, fish oil 5.6%, tapioca 22.25%, kilnitamin 17.7%, microcrystalline cellulose 14.7%, chlorine
Change choline 1%, calcium dihydrogen phosphate 1%, glutamic acid 0.25~1.25%, multi-vitamins 1%, composite ore substance 1%, hydroxyl dried meat ammonia
Acid 0.25~1.25%.
The effect detection of feed of the present invention：
Culture experiment is bordered on the sea in University Of Shantou of Shantou, Guangdong Nanao County village of the stockaded village Wu Ping Nan'ao in the steck net cage of experiment station sea
It carries out, fry is purchased from Raoping, Guangdong Province.Aquaculture net cage is 1 × 1 × 1.5m, there is cover net, to prevent escape and aquatic bird.By embodiment 4
The hydroxyproline of formula addition corresponding amount, design hydroxyproline content is respectively 0,0.25,0.5,0.75,1,1.25% 6 ladder
The feed of degree, surveying hydroxyproline content in feed is respectively 0.04%, 0.27%, 0.53%, 0.78%, 1.06% He
1.29%, number be I~VI (can also mark be, CH1, CH2, CH3, CH4 and CH5), feed formula (number as shown in table 1
Value is percentage), every group of 3 repetitions, each 30 tail fish of repetition.Temperature during cultivation is 20~26 DEG C, and salinity is 30 ‰ left
The right side, for dissolved oxygen content in 6mg/L or more, the culture-cycle is 60 days.
It after culture experiment, weighs to all fishes of each test group, calculates growth performance index, 2 are shown in Table, by table
2 growth indexes can be seen that the raising with hydroxyproline pitch-based sphere, Nibea coibor rate of body weight gain, specific growth rate and
Protein efficiency ratio, PER presentation first increases the trend reduced afterwards, and raised trend after first reducing is presented in feed coefficient, wherein III group of life
Long performance is best.
It randomly chooses 5 tail fishes from each repetition, after jugular vein sacrificed by exsanguination, then takes out swim bladder tissue, shave surface
Loaded in hermetic bag after fat, -20 DEG C of refrigerators are saved, in case measurement air bladder nutritional ingredient and air bladder collagen content.By table 3
Air bladder trophic component can be seen that hydroxyproline and not make significant difference to air bladder trophic component, but after hydroxyproline addition, light color is yellow
Crude protein content has raised trend in aunt's fish air bladder.It can be seen that by 4 air bladder amino acid content of table, it, must after adding hydroxyproline
Methionine and lysine presentation in amino acid is needed first to increase the trend reduced afterwards, maximum value respectively appears in the IIIth and the IVth group,
And significant difference, in nonessential amino acid, after adding hydroxyproline, glutamic acid, glycine and proline content are increased, hydroxyl
Proline does not make significant difference to other amino acid.
By 5 air bladder collagen content of table it is found that with hydroxyproline pitch-based sphere raising, Nibea coibor air bladder is total
The trend for first increasing and reducing afterwards is presented in collagen and soluble collagen content, and occurs maximum value at V group, and aobvious
It writes and is higher than I group (control group).This may be since the raising of hydroxyprolin levels inhibits the degradation of collagen, and not necessarily
Improve the synthesis capability of collagen.
The feed formula that the number of table 1 is I~VI
Note：The U.S. a white fish meal, gross protein value 64.7%, crude fat content 10%；
B fish oil, tapioca are commercial product, can be from internal sources market purchasing；
C kilnitamin (g/100g feed):Glycine, 13.29；Alanine, 3.63；Arginine, 0.28；Phenylpropyl alcohol ammonia
Acid, 0.31；Threonine, 0.18, it can be from internal sources market purchasing；
The auxiliary materials such as d glutamic acid, multi-vitamins, composite mineral matter, calcium dihydrogen phosphate, choline chloride can be from internal feed factory
Buying.
With hydroxyproline content (percentage) added in feed for independent variable, total collagen egg in Nibea coibor air bladder
White (TC) content can obtain the total collagen content (y) of air bladder and hydroxyproline additive amount (x) by regression analysis for dependent variable
Regression equation：Y=-50.157x2+94.08x+573.85(R2=0.8446).By regression equation derivation it is found that working as hydroxyl dried meat ammonia
When sour additive amount is 0.94%, the total collagen content of Nibea coibor juvenile fish air bladder has theoretic maximum value, such as Fig. 1 institute
Show.
Influence of 2 hydroxyproline of table to Nibea coibor growth performance
Note：Data are average value ± standard error；In data of going together, no identical lowercase labeler indicates mutual difference
Significantly (P<0.05)
Influence of 3 hydroxyproline of table to Nibea coibor air bladder nutritional ingredient
Note：Data are average value ± standard error；In data of going together, no identical lowercase labeler indicates mutual difference
Significantly (P<0.05)
Influence of 4 hydroxyproline of table to Nibea diacanthus air bladder amino acid content
Note：EAA：Essential amino acid, NEAA：Nonessential amino acid.
Data are average value ± standard error；In data of going together, no identical lowercase labeler indicates that mutual difference is aobvious
(P<0.05)
Influence of 5 hydroxyproline of table to Nibea coibor air bladder collagen content
Figure it is seen that after addition hydroxyproline feeds Nibea coibor juvenile fish, very to each tissue pH activity influence
Greatly.Being affected maximum is swim bladder tissue, there is significant difference (P between each group<0.05), maximum value appears in CH3
Group；Secondly musculature has significant difference (P in addition to CH1 and CH4 group difference is not significant between remaining each group<0.05), most
Big value appears in CH2 group.Being affected the smallest is liver organization, in addition to CH1 and CH2, CH0 and CH5 group difference is not significant,
There is significant difference (P between remaining each group<0.05), maximum value appears in CH1 group.
From figure 3, it can be seen that pH activity is significant after addition hydroxyproline feeds Nibea coibor juvenile fish, in serum increases (P
<0.05), and as the increase of additive amount is in rising trend, maximum value appears in CH5 group.
It can be obtained by table 6, after addition hydroxyproline feeds Nibea coibor juvenile fish, blood calcium persistently drops in each group serum
It is low, in addition to CH2, CH3, CH4 group, remaining each group significant difference (P<0.05).Content of triglyceride decreases drop compared with CH0 group
It is low, but the not significant (P of difference>0.05), illustrate that Hyp additive amount may not influence it.Total cholesterol level is similar with blood calcium,
Without significant difference (P between each group>0.05), but addition group CH1 to CH5 group decreases compared with control group CH0.
Table 6, which adds hydroxyproline, influences SC, TG and T-CH content in Nibea coibor juvenile fish serum
Note：Data are mean+SD (n=3)；With number of rows in, different lowercase labelers indicate mutual
Significant difference (P<0.05)
As shown in Table 7, after addition hydroxyproline feeds Nibea coibor juvenile fish, the TG content in each group liver is lower than control
Group CH0, but the not significant (P of difference>0.05).T-CH content is risen, but each group is also without significant difference (P>0.05).
Table 7, which adds hydroxyproline, influences (% weight in wet base) to TG and T-CH content in Nibea coibor juvenile fish liver
Note：Data are mean+SD (n=3)；With number of rows in, different lowercase labelers indicate mutual
Significant difference (P<0.05)
The result of additional embodiment 1-3 data analysis is similar to Example 4.The SUR of Nibea coibor is in 90.00%-
Between 96.67%, and the not significant (P of difference between each group>0.05).With the raising of Hyp content, the WGR of Nibea coibor and
SGR significantly increases (P<0.05).Each group CP, CA, CL content is not significantly different (P>0.05).SC and TG content is with feeding in serum
The raising of Hyp content is remarkably decreased (P in material<0.05), T-CH content is also on a declining curve, but the not significant (P of difference>
0.05), feed difference graded levels Hyp content has significant shadow to amino acid composition in Nibea coibor juvenile fish muscle, air bladder
Ring (P<0.05).ASC and TC content has significant raising (P compared with CH0 group in CH4 group air bladder<0.05), to PH activity in each group air bladder
With significantly affecting (P<0.05).Free serum Hyp content and the total Hyp content of liver are aobvious with the raising of feed Hyp level
Write increase, and with the horizontal correlation of feed Hyp.The change of fish free serum Hyp and the total Hyp content of liver and muscle
Change the variation that protein (especially collagen) may be caused to be metabolized.But in muscle total collagen content not with feed
Middle Hyp is increased and is changed.
Claims (7)
1. a kind of artifical compound feed for improving Nibea coibor air bladder quality, including fish meal, fish oil, tapioca and auxiliary material,
It is characterized in that, further including the hydroxyproline that weight percentage is 0.25~1.25%；
The auxiliary material is selected from by choline chloride, microcrystalline cellulose, multi-vitamins, composite mineral matter, calcium dihydrogen phosphate, paddy ammonia
The group that acid, kilnitamin and their one or more of mixtures are constituted；
Wherein, the feed is made of the nutritional ingredient of following weight percent：Crude protein 42.78~42.96, crude fat 8.47
~8.59, remainder is carbohydrate, crude fibre, ash content and moisture.
2. improving the artifical compound feed of Nibea coibor air bladder quality according to claim 1, which is characterized in that the hydroxyl
The weight percent of proline is 1%.
3. improving the artifical compound feed of Nibea coibor air bladder quality according to claim 1, which is characterized in that including weight
Measure the glutamic acid that percentage composition is 0.25~1.25%.
4. improving the artifical compound feed of Nibea coibor air bladder quality according to claim 1, which is characterized in that main packet
Include the raw material of following weight percentage：Fish meal 32~36%, fish oil 5.6%, tapioca 20~24%, kilnitamin 17
~19%, microcrystalline cellulose 13~15%, choline chloride 1%, calcium dihydrogen phosphate 1%, glutamic acid 0.25~1.25%, compound dimension
Raw element 1%, composite ore substance 1%, hydroxyproline 0.25~1.25%.
5. improving the artifical compound feed of Nibea coibor air bladder quality according to claim 1, which is characterized in that main packet
Include the raw material of following weight percentage：Fish meal 34.5%, fish oil 5.6%, tapioca 22.25%, kilnitamin
17.7%, microcrystalline cellulose 14.7%, choline chloride 1%, calcium dihydrogen phosphate 1%, glutamic acid 0.25%, multi-vitamins 1%,
Composite ore substance 1%, hydroxyproline 1%.
6. the preparation of any one of -5 artifical compound feeds for improving Nibea coibor air bladder quality according to claim 1,
It is characterized in that, mainly includes：By in addition to fish oil other raw materials and auxiliary material crush in advance and cross 80~120 meshes, after mixing plus
Entering moisture, extruding is pelletized after mixing, and swelling temperature is 100~120 DEG C, finally fish oil is sprayed in feed, after fully absorbing,
Up to finished feed.
7. the application of any one of -5 artifical compound feeds for improving Nibea coibor air bladder quality according to claim 1,
It is characterized in that, for improving Nibea coibor air bladder quality.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114009633A (en) * | 2021-11-24 | 2022-02-08 | 汕头大学 | Feed premix for improving quality and muscle quality of air bladder of light-colored spotted maigre |
| CN114343074A (en) * | 2021-12-03 | 2022-04-15 | 汕头大学 | Feed containing broad bean water extract and preparation method and application thereof |
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| CN104543565A (en) * | 2015-01-13 | 2015-04-29 | 浙江省海洋水产研究所 | Young nibea albiflora compound feed and preparation method thereof |
| CN107373208A (en) * | 2017-07-29 | 2017-11-24 | 合肥申仁养殖有限公司 | A kind of mixed feed for promoting spotted maigre growth |
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- 2018-09-03 CN CN201811022736.3A patent/CN108850734A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104543565A (en) * | 2015-01-13 | 2015-04-29 | 浙江省海洋水产研究所 | Young nibea albiflora compound feed and preparation method thereof |
| CN107373208A (en) * | 2017-07-29 | 2017-11-24 | 合肥申仁养殖有限公司 | A kind of mixed feed for promoting spotted maigre growth |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114009633A (en) * | 2021-11-24 | 2022-02-08 | 汕头大学 | Feed premix for improving quality and muscle quality of air bladder of light-colored spotted maigre |
| CN114009633B (en) * | 2021-11-24 | 2023-09-26 | 汕头大学 | Feed premix for improving quality of light-colored spotted maigre swim bladder and muscle quality |
| CN114343074A (en) * | 2021-12-03 | 2022-04-15 | 汕头大学 | Feed containing broad bean water extract and preparation method and application thereof |
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Application publication date: 20181123 |