CN104531805B - Promote the yam oligosaccharide mixture and preparation method of proliferation of intestinal probiotics - Google Patents
Promote the yam oligosaccharide mixture and preparation method of proliferation of intestinal probiotics Download PDFInfo
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Abstract
The invention discloses a kind of yam oligosaccharide mixture and preparation method for promoting proliferation of intestinal probiotics, (1) miniaturization Chinese yam magma；(2) medium temperature alpha amylase is added in Chinese yam magma and carries out first time enzymolysis；(3) after first time digests, add Fungal Alpha amylase and beta glucan enzyme carries out second and digested；(4) after being digested to second gained mixing molasses decolourized, desalination, concentration, drying, obtain yam oligosaccharide mixture.The present invention not only by starch by medium temperature alpha amylase enzymatic starch be macromolecular amylin, and then using Fungal Alpha amylase by macromolecular amylin enzymolysis be malto-oligosaccharide, it is synchronous to use beta glucan enzyme degraded cellulose, obtain reaching with malto-oligosaccharide and cellooligosaccharide content more than 80% yam oligosaccharide mixture.The yam oligosaccharide mixture can be used as raw-food material and medicine auxiliary material, and particle, powder, tablet, capsule or beverage etc. are configured to one or more food and pharmaceutically acceptable carrier.
Description
Technical field
The present invention relates to a kind of yam oligosaccharide mixture, more particularly to one kind to have promotion proliferation of intestinal probiotics effect
Yam oligosaccharide mixture, the invention further relates to the preparation method of the yam oligosaccharide mixture.
Background technology
Chinese yam is the rhizome of Dioscoreaceae sprawling herbs plant Chinese yam for many years, is the traditional integration of drinking and medicinal herbs food in China, also belongs to
In the medicine-food two-purpose resource that the Ministry of Public Health of China announces.《Compendium of Materia Medica》Record " Chinese yam is warm-natured, sweet taste, kidney-nourishing gas, strengthening the spleen and stomach,
Stopping leak dysentery, resolving sputum saliva, moisten fur ", the coordinating intestines and stomach function of Chinese yam has gained public acceptance；In addition, modern study confirms Chinese yam tool
There are the multiple efficacies such as anti-oxidant, reducing blood lipid, reducing blood lipid, antitumor, immunological regulation.
Oligosaccharide, which has been shown to have, to be improved micro-ecological environment in human body, improves blood lipid metabolism, enhancing immunologic function, delays
Aging and a variety of pharmacological activity such as antitumor, especially by promoting beneficial bacteria of intestinal tract growth to improve human body Tiny ecosystem ring
Border, the function of coordinating intestines and stomach are most notable, it is shown that good development prospect.
Oligosaccharide is the important substance basis of Chinese yam coordinating intestines and stomach function, and naturally occurring oligosaccharide content is not in Chinese yam
Height, but containing abundant starch and cellulose in Chinese yam, therefore, using Chinese yam as raw material, preparing one kind can promote enteron aisle beneficial
The yam oligosaccharide mixture of raw bacterium propagation, not only conforms with demand of the people to health food, is also the profit of China's Chinese yam resource
With providing a kind of new way.
The content of the invention
An object of the present invention is to provide a kind of preparation method of yam oligosaccharide mixture.
The second object of the present invention is to provide a kind of yam oligosaccharide mixture that can promote proliferation of intestinal probiotics.
One of the object of the invention can be achieved through the following technical solutions：A kind of preparation side of yam oligosaccharide mixture
Method, comprise the following steps：
(1), miniaturization Chinese yam magma；
(2) mesophilicα-diastase, is added in Chinese yam magma and carries out first time enzymolysis；
(3), after first time digests, add fungal alpha-amylase and 1,4 beta-glucanase carries out second and digested；
(4) after, being digested to second gained mixing molasses decolourized, desalination, concentration, drying, obtain yam oligosaccharide and mix
Compound.
The present invention selects the fresh Chinese yam of the full rhizome without peeling, to utilize the starch and yam skin enriched in yam meat
In enrich cellulose.
The process of miniaturization Chinese yam magma of the present invention：Fresh yam cleans stripping and slicing, adds the cleaning of Chinese yam 5~8 times of weight of block
Water is beaten, and is subsequently placed in homogenizer the homogeneous under 10~15MPa pressure, obtains 250~350 μm of solids average grain diameter
The yam pulp of (Mastersizer3000 particle diameter distributions instrument measure), micro- penetrate further is carried out by microjet nanometer homogenizer
Stream process, microjet treatment conditions are 90~120MPa of pressure, 30 DEG C of temperature, obtain 100~150 μm of solids average grain diameter
The yam pulp of (Mastersizer3000 particle diameter distributions instrument measure).
Enzymolysis detailed process for the first time of the invention：Using 0.1M hydrochloric acid and 0.1MNa2CO3It is 6.0 to adjust Chinese yam magma pH value
~6.5；Being heated to 90~95 DEG C keeps 10~15min complete to the gelatinization of Chinese yam magma；Medium temperature is added when being cooled to 75~85 DEG C
Alpha-amylase simultaneously mixes, and in terms of dry in Chinese yam magma, addition is 1.8~3.0U/g；Kept for 75~85 DEG C, 20~40 points
It is 12%~15% that clock, which carries out enzymolysis to slurries DE values for the first time,；It is brought rapidly up to 95~100 DEG C, the work of enzyme deactivation in 2~3 minutes；It is cold
But to 45~55 DEG C.
Second of enzymolysis detailed process of the present invention：Using 0.1M salt adjust the slurry pH value after digesting for the first time as 5.0~
5.5；Add fungal alpha-amylase and 1,4 beta-glucanase, in the yam pulp after digesting for the first time in terms of dry, addition is
0.9~1.5U/g of 8~12U/g of fungal alpha-amylase and 1,4 beta-glucanase, carry out second and digest 3~4 hours to slurries DE values
For 23%~27%；It is brought rapidly up to 95~100 DEG C, the work of enzyme deactivation in 2~3 minutes；Obtain Chinese yam mixing molasses.
The detailed process that the present invention is decolourized：It is mixed using AB-8 macroreticular resins and Chinese yam malto-oligosaccharide and cellooligosaccharide etc.
Syrup is closed with 1：5~6 weight after 1~2hr of concussion absorption, filters than well mixed.
The detailed process of desalination of the present invention：Combined using 001 × 7 cationic ion-exchange resin and D201 anion exchange resin
Desalination, it will be inhaled by the mixing molasses to decolourize with 1~1.5ml/min flow velocity by 001 × 7 cationic ion-exchange resin chromatographic column
Na in attached syrup+、Mg+、K+、H+Deng cation, the mixing molasses efflux for sloughing above-mentioned cation is collected, with 1~1.5ml/
Min flow velocity is by the Cl in D201 anion exchange resin chromatographic column absorption mixing molasses-、CO3 2-、NO3-、OH-Deng it is cloudy from
Son, it is the refined mixing molasses Jing Guo desalination to collect efflux.
Yam oligosaccharide mixture prepared by a kind of the inventive method, the yam oligosaccharide mixture can be used as raw-food material
With medicine auxiliary material, particle, powder, tablet, capsule or drink are configured to one or more food and pharmaceutically acceptable carrier
Material etc..
Beneficial effects of the present invention：
(1) the Chinese yam magma by mashing and homogeneous further carried out microjet and handle to obtain miniaturization mountain by the present invention
Medicine magma, the average grain diameter of the insoluble composition granule such as starch, cellulose is by 250~350 μ after common homogenization in yam pulp
M drops to 100~150 μm, has increased considerably the surface area of the substrate particles such as starch, cellulose, and it is anti-to be favorably improved enzymolysis
Answer efficiency；
(2) the Chinese yam raw material that the present invention uses is the full rhizome of Chinese yam without peeling, makes full use of what is enriched in yam meat
The cellulose enriched in starch and yam skin, it is not only macromolecular amylin by mesophilicα-diastase enzymatic starch by starch, enters
And it is malto-oligosaccharide to use fungal alpha-amylase to digest macromolecular amylin, and in fungal alpha-amylase enzymolysis macromolecular paste
It is synchronous to be dropped using 1,4 beta-glucanase the characteristics of using 1,4 beta-glucanase and close fungal alpha-amylase enzymatic hydrolysis condition while smart
Cellulose is solved, obtains the yam oligosaccharide mixture using malto-oligosaccharide and cellooligosaccharide as main component；Its oligosaccharide is (main
To be malto-oligosaccharide and cellooligosaccharide) content reaches more than 80% (percentage by weight).
(3) the yam oligosaccharide mixture for preparing of the present invention is due to containing a variety of oligomeric saccharic compositions, compared to one-component
Oligomeric sugar product have more wide spectrum promotion proliferation of intestinal probiotics effect, to lactobacillus acidophilus, streptococcus thermophilus, protect plus
Six kinds of beneficial bacteria of intestinal tract such as Leah lactobacillus, Lactobacillus plantarum, animal bifidobacteria, bifidobacterium adolescentis all have excellent body
Outer growth promoting function.
(4) yam oligosaccharide mixture prepared by the present invention also can be Chinese yam as the health-care food ingredient of high-quality
New approach has been opened up in deep processing.
Embodiment
Following examples are used merely to explain the present invention, are not intended to limit protection scope of the present invention.The art it is common
The purpose of the present invention can be achieved according to scope disclosed above in technical staff.
Embodiment 1
Fresh Chinese yam cleans stripping and slicing, and the clean water for adding Chinese yam 6 times of weight of block is beaten, be subsequently placed in homogenizer in
Homogeneous under 14MPa pressure, the yam pulp of 325.46 μm of solids average grain diameter is obtained, further pass through microjet nanometer homogeneous
Machine carries out microjet processing, and microjet treatment conditions are pressure 110MPa, 30 DEG C of temperature, obtain solids average grain diameter 130.27
The miniaturization yam pulp of μm (Mastersizer3000 particle diameter distributions instrument measure)；
Using 0.1M hydrochloric acid and 0.1MNa2CO3It is 6.20 to adjust above-mentioned miniaturization yam pulp pH value, is heated to 90 DEG C of guarantors
It is complete to the gelatinization of Chinese yam magma to hold 15min, mesophilicα-diastase 2.0U/g is added when being cooled to 85 DEG C and is mixed, above-mentioned addition
In terms of dry in Chinese yam magma, it is 13.5% to be kept for 85 DEG C carry out within 25 minutes enzymolysis to slurries DE values for the first time, then rapid
97 DEG C of enzyme deactivations in 3 minutes are warming up to live, after the completion of be cooled to 55 DEG C, pH value is adjusted as 5.5 using 0.1M salt, while add fungi α-shallow lake
Powder enzyme 9.0U/g and 1,4 beta-glucanase 1.1U/g carry out second and digested, and above-mentioned addition is with the yam pulp after digesting for the first time
Middle dry meter, second enzymolysis 4.0 hours to slurries DE values are 26.5%, are brought rapidly up to 100 DEG C of enzyme deactivations in 2 minutes living to obtain
The mixing molasses crude product such as Chinese yam malto-oligosaccharide and cellooligosaccharide；
By the mixing molasses crude product such as AB-8 macroreticular resins and above-mentioned Chinese yam malto-oligosaccharide and cellooligosaccharide with 1：5 weight
Measure than well mixed, after concussion absorption 2hr, suction filtration obtains the mixing such as Chinese yam malto-oligosaccharide and cellooligosaccharide by decolouring
Syrup；The above-mentioned mixing molasses by decolourizing are passed through into 001 × 7 cationic ion-exchange resin chromatographic column with 1.5ml/min flow velocity,
It is to take off the mixing molasses such as decationizing yam oligosaccharide to collect efflux；The above-mentioned decationizing yam oligosaccharide etc. that takes off is mixed
Close syrup and anion therein is adsorbed by D201 anion exchange resin chromatographic column with 1.5ml/min flow velocity, collect outflow
Liquid is the refined yam oligosaccharide mixing molasses Jing Guo desalination.
Above-mentioned refined yam oligosaccharide mixing molasses are freeze-dried after vacuum concentration, enteron aisle can be promoted by obtaining one kind
The yam oligosaccharide mixture of proliferation of probiotics, wherein malto-oligosaccharide content are 67.25%, cellooligosaccharide content is
15.83% (being weight percentage).
Embodiment 2
Fresh Chinese yam cleans stripping and slicing, and the clean water for adding Chinese yam 7 times of weight of block is beaten, be subsequently placed in homogenizer in
Homogeneous under 15MPa pressure, the yam pulp of 285.49 μm of solids average grain diameter is obtained, further pass through microjet nanometer homogeneous
Machine carries out microjet processing, and microjet treatment conditions are pressure 95MPa, 30 DEG C of temperature, obtain the μ of solids average grain diameter 128.12
M (Mastersizer3000 particle diameter distributions instrument measure) miniaturization yam pulp；
Using 0.1M hydrochloric acid and 0.1MNa2CO3It is 6.50 to adjust above-mentioned miniaturization yam pulp pH value, is heated to 92 DEG C of guarantors
It is complete to the gelatinization of Chinese yam magma to hold 12min, mesophilicα-diastase 1.9U/g is added when being cooled to 75 DEG C and is mixed, above-mentioned addition
In terms of dry in Chinese yam magma, it is 12.5% to be kept for 75 DEG C carry out within 40 minutes enzymolysis to slurries DE values for the first time, then rapid
100 DEG C of enzyme deactivations in 2 minutes are warming up to live, after the completion of be cooled to 48 DEG C, pH value is adjusted as 5.0 using 0.1M salt, at the same add fungi α-
Amylase 11.0U/g and 1,4 beta-glucanase 1.5U/g carry out second and digested, and above-mentioned addition is with the Chinese yam after digesting for the first time
Dry meter in slurries, second enzymolysis 3.0 hours to slurries DE values are 24.3%, are brought rapidly up to 95 DEG C of work of enzyme deactivations in 3 minutes
Obtain the mixing molasses crude product such as Chinese yam malto-oligosaccharide and cellooligosaccharide；
By the mixing molasses crude product such as AB-8 macroreticular resins and above-mentioned Chinese yam malto-oligosaccharide and cellooligosaccharide with 1：6 weight
Measure than well mixed, after concussion absorption 1hr, suction filtration obtains the mixing such as Chinese yam malto-oligosaccharide and cellooligosaccharide by decolouring
Syrup；The above-mentioned mixing molasses by decolourizing are passed through into 001 × 7 cationic ion-exchange resin chromatographic column with 1.2ml/min flow velocity,
It is to take off the mixing molasses such as decationizing yam oligosaccharide to collect efflux；The above-mentioned decationizing yam oligosaccharide etc. that takes off is mixed
Close syrup and anion therein is adsorbed by D201 anion exchange resin chromatographic column with 1.3ml/min flow velocity, collect outflow
Liquid is the refined yam oligosaccharide mixing molasses Jing Guo desalination.
Above-mentioned refined yam oligosaccharide mixing molasses are freeze-dried after vacuum concentration, enteron aisle can be promoted by obtaining one kind
The yam oligosaccharide mixture of proliferation of probiotics, wherein malto-oligosaccharide content are 65.74%, cellooligosaccharide content is
16.87% (being weight percentage).
Embodiment 3
Fresh Chinese yam cleans stripping and slicing, and the clean water for adding Chinese yam 8 times of weight of block is beaten, be subsequently placed in homogenizer in
Homogeneous under 13MPa pressure, the yam pulp of 312.36 μm of solids average grain diameter is obtained, further pass through microjet nanometer homogeneous
Machine carries out microjet processing, and microjet treatment conditions are pressure 105MPa, 30 DEG C of temperature, obtain solids average grain diameter 136.75
The miniaturization yam pulp of μm (Mastersizer3000 particle diameter distributions instrument measure)；
Using 0.1M hydrochloric acid and 0.1MNa2CO3It is 6.00 to adjust above-mentioned miniaturization yam pulp pH value, is heated to 95 DEG C of guarantors
It is complete to the gelatinization of Chinese yam magma to hold 10min, mesophilicα-diastase 2.2U/g is added when being cooled to 80 DEG C and is mixed, above-mentioned addition
In terms of dry in Chinese yam magma, it is 14.0% to be kept for 80 DEG C carry out within 30 minutes enzymolysis to slurries DE values for the first time, then rapid
95 DEG C of enzyme deactivations in 3 minutes are warming up to live, after the completion of be cooled to 50 DEG C, pH value is adjusted as 5.2 using 0.1M salt, while add fungi α-shallow lake
Powder enzyme 10.0U/g and 1,4 beta-glucanase 0.9U/g carry out second and digested, and above-mentioned addition is with the Chinese yam pulp after digesting for the first time
Dry meter in liquid, second enzymolysis 3.5 hours to slurries DE values are 25.7%, are brought rapidly up to 98 DEG C of enzyme deactivations in 3 minutes living
To the mixing molasses crude product such as Chinese yam malto-oligosaccharide and cellooligosaccharide；
By the mixing molasses crude product such as AB-8 macroreticular resins and above-mentioned Chinese yam malto-oligosaccharide and cellooligosaccharide with 1：6 weight
Measure than well mixed, after concussion absorption 1.5hr, it is mixed that suction filtration obtains Chinese yam malto-oligosaccharide and cellooligosaccharide by decolouring etc.
Close syrup；The above-mentioned mixing molasses by decolourizing are chromatographed with 1.0ml/min flow velocity by 001 × 7 cationic ion-exchange resin
Post, it is to take off the mixing molasses such as decationizing yam oligosaccharide to collect efflux；Decationizing yam oligosaccharide is taken off by above-mentioned
Collected Deng mixing molasses with 1.0ml/min flow velocity by D201 anion exchange resin chromatographic column absorption anion therein
Efflux is the refined yam oligosaccharide mixing molasses Jing Guo desalination.
Above-mentioned refined yam oligosaccharide mixing molasses are freeze-dried after vacuum concentration, enteron aisle can be promoted by obtaining one kind
The yam oligosaccharide mixture of proliferation of probiotics, wherein malto-oligosaccharide content are 69.34%, cellooligosaccharide content is
13.57% (being weight percentage).
The continuous mode of DE values is as follows in the various embodiments described above：DE values %=(content of reducing sugar/dry matter content) ×
100%；Content of reducing sugar is determined with 3,5. dinitrosalicylic acid systems (DNS methods), and amount of dry matter is determined with abbe's refractometer；Wherein
Concentration measured by abbe's refractometer refers to the grams of the dry contained in every 100g liquid glucoses, and content of reducing sugar refers to 100g sugar
The grams of contained reduced sugar in liquid.
Take the yam oligosaccharide mixture obtained by above-described embodiment 1 to carry out the external stimulation effect of beneficial bacteria of intestinal tract to comment
Valency, do following test example：
The above-mentioned yam oligosaccharide mixture substitution wherein grape of various concentrations is added in modified MRS basal medium
Sugar, its ultimate density is respectively reached 0.5%, 1%, 2%, (wherein 2% is that yam oligosaccharide substitutes MRS basal mediums completely
Middle glucose)；Lactobacillus acidophilus after activation, streptococcus thermophilus, lactobacillus bulgaricus and Lactobacillus plantarum are pressed 5% respectively
Inoculum concentration be connected to respectively in above culture medium, take out zymotic fluid after 37 DEG C of quiescent culture 24hr, determine the viable bacteria in culture medium
Number.Using basal medium as control, compare various concentrations yam oligosaccharide to lactobacillus acidophilus, streptococcus thermophilus, Bulgaria
The multiplication effect of lactobacillus and Lactobacillus plantarum.Experimental result is shown in Table 1
The above-mentioned yam oligosaccharide mixture that various concentrations are added in Bifidobacterium basal medium substitutes Portugal therein
Grape sugar, makes the ultimate density of yam oligosaccharide mixture respectively reach 0.25%, 0.5%, and the Bifidobacterium after activation is pressed into 5%
Inoculum concentration be connected to respectively in above culture medium, take out zymotic fluid after 37 DEG C of Anaerobic culturel 24hr, determine the viable bacteria in culture medium
Number.Using basal medium as control, compare increasing of the various concentrations yam oligosaccharide to bifidobacterium adolescentis and animal bifidobacteria
Grow effect.
Influence of the yam oligosaccharide mixture of table 1 to lactic acid bacteria growth in vitro
Influence of the yam oligosaccharide of table 2 to Bifidobacterium growth in vitro
From table 1, glucose in MRS basal mediums, inoculation are substituted with Different adding amount yam oligosaccharide mixture
After cultivating 24hr, the work of 4 kinds of lactic acid bacterias such as its lactobacillus acidophilus, streptococcus thermophilus, lactobacillus bulgaricus and Lactobacillus plantarum
Bacterium number is above the MRS basal mediums for not substituting glucose with yam oligosaccharide mixture, and with substitution glucose amount
It is in increase trend to increase its viable count, and when yam oligosaccharide mixture addition is 2.0%, i.e., substitution MRS is cultivated on basis completely
In base during glucose, the viable count highest of its lactic acid bacteria；Illustrate that yam oligosaccharide adds to lactobacillus acidophilus, streptococcus thermophilus, guarantor
4 kinds of lactic acid bacterias such as Leah lactobacillus and Lactobacillus plantarum have good growth promoting function.
From table 2, respectively with the yam oligosaccharide mixture substitution Bifidobacterium culture of 0.25%, 0.5% addition
The glucose of same amount in base, after inoculated and cultured 24hr, the work of 2 kinds of Bifidobacteriums such as its bifidobacterium adolescentis, animal bifidobacteria
Bacterium number is all remarkably higher than basal medium, and as its viable count of increase of substitution glucose amount is in obvious increase trend, explanation
Yam oligosaccharide has good growth promoting function to 2 kinds of Bifidobacteriums such as bifidobacterium adolescentis, animal bifidobacteria.
4 kinds of lactic acid bacterias such as lactobacillus acidophilus, streptococcus thermophilus, lactobacillus bulgaricus, Lactobacillus plantarum and animal bifid
2 kinds of Bifidobacteriums such as bacillus, bifidobacterium adolescentis are beneficial bacteria of intestinal tract, yam oligosaccharide mixture prepared by other embodiment
By above-mentioned same experiment process, approximate experimental result can be also obtained, is not repeated one by one.Test result indicates that the present invention
The yam oligosaccharide mixture of acquisition has good promotion proliferation of intestinal probiotics effect.
The yam oligosaccharide mixture of above-mentioned preparation can be used as raw-food material and medicine auxiliary material, with one or more food and
Pharmaceutically acceptable carrier is configured to particle, powder, tablet, capsule or beverage etc., the health food as high-quality.
Claims (3)
1. a kind of preparation method of the yam oligosaccharide mixture of promotion proliferation of intestinal probiotics, it is characterised in that including following step
Suddenly：
(1) fresh Chinese yam clean stripping and slicing, add Chinese yam 6 times of weight of block clean water be beaten, be subsequently placed in homogenizer in
Homogeneous under 14MPa pressure, yam pulp is obtained, microjet processing, microjet are further carried out by microjet nanometer homogenizer
Treatment conditions are pressure 110MPa, 30 DEG C of temperature, obtain miniaturization yam pulp；
(2) 0.1M hydrochloric acid and 0.1MNa are used2CO3It is 6.20 to adjust above-mentioned miniaturization yam pulp pH value, is heated to 90 DEG C of holdings
15min is complete to the gelatinization of Chinese yam magma, and mesophilicα-diastase 2.0U/g is added when being cooled to 85 DEG C and is mixed, above-mentioned addition with
Dry meter in Chinese yam magma, it is 13.5% to be kept for 85 DEG C carry out within 25 minutes enzymolysis to slurries DE values for the first time, then rapid to rise
Temperature to 97 DEG C of enzyme deactivations in 3 minutes are lived, after the completion of be cooled to 55 DEG C, pH value is adjusted as 5.5 using 0.1M salt, while add fungi alphalise starch
Enzyme 9.0U/g and 1,4 beta-glucanase 1.1U/g carry out second and digested, and above-mentioned addition is with the yam pulp after digesting for the first time
Dry meter, second enzymolysis 4.0 hours to slurries DE values are 26.5%, are brought rapidly up to 100 DEG C, enzyme deactivation in 2 minutes living to obtain
Chinese yam malto-oligosaccharide and cellooligosaccharide mixing molasses crude product；
(3) by AB-8 macroreticular resins and above-mentioned Chinese yam malto-oligosaccharide and cellooligosaccharide mixing molasses crude product with 1：5 weight
Than well mixed, after concussion absorption 2hr, suction filtration obtains the Chinese yam malto-oligosaccharide and cellooligosaccharide mixed sugar by decolouring
Slurry；The above-mentioned mixing molasses by decolourizing are passed through into 001 × 7 cationic ion-exchange resin chromatographic column with 1.5ml/min flow velocity, received
It is to take off decationizing yam oligosaccharide mixing molasses to collect efflux；Decationizing yam oligosaccharide mixing molasses are taken off by above-mentioned
Anion therein is adsorbed by D201 anion exchange resin chromatographic column with 1.5ml/min flow velocity, collecting efflux is
By the refined yam oligosaccharide mixing molasses of desalination.
2. a kind of preparation method of the yam oligosaccharide mixture of promotion proliferation of intestinal probiotics, it is characterised in that including following step
Suddenly：
(1) fresh Chinese yam clean stripping and slicing, add Chinese yam 7 times of weight of block clean water be beaten, be subsequently placed in homogenizer in
Homogeneous under 15MPa pressure, yam pulp is obtained, microjet processing, microjet are further carried out by microjet nanometer homogenizer
Treatment conditions are pressure 95MPa, 30 DEG C of temperature, obtain miniaturization yam pulp；
(2) 0.1M hydrochloric acid and 0.1MNa are used2CO3It is 6.50 to adjust above-mentioned miniaturization yam pulp pH value, is heated to 92 DEG C of holdings
12min is complete to the gelatinization of Chinese yam magma, and mesophilicα-diastase 1.9U/g is added when being cooled to 75 DEG C and is mixed, above-mentioned addition with
Dry meter in Chinese yam magma, it is 12.5% to be kept for 75 DEG C carry out within 40 minutes enzymolysis to slurries DE values for the first time, then rapid to rise
Temperature to 100 DEG C of enzyme deactivations in 2 minutes are lived, after the completion of be cooled to 48 DEG C, pH value is adjusted as 5.0 using 0.1M salt, while add fungi α-shallow lake
Powder enzyme 11.0U/g and 1,4 beta-glucanase 1.5U/g carry out second and digested, and above-mentioned addition is with the Chinese yam pulp after digesting for the first time
Dry meter in liquid, second enzymolysis 3.0 hours to slurries DE values are 24.3%, are brought rapidly up to 95 DEG C, enzyme deactivation in 3 minutes living
To Chinese yam malto-oligosaccharide and cellooligosaccharide mixing molasses crude product；
(3) by AB-8 macroreticular resins and above-mentioned Chinese yam malto-oligosaccharide and cellooligosaccharide mixing molasses crude product with 1：6 weight
Than well mixed, after concussion absorption 1hr, suction filtration obtains the Chinese yam malto-oligosaccharide and cellooligosaccharide mixed sugar by decolouring
Slurry；The above-mentioned mixing molasses by decolourizing are passed through into 001 × 7 cationic ion-exchange resin chromatographic column with 1.2ml/min flow velocity, received
It is to take off decationizing yam oligosaccharide mixing molasses to collect efflux；Decationizing yam oligosaccharide mixing molasses are taken off by above-mentioned
Anion therein is adsorbed by D201 anion exchange resin chromatographic column with 1.3ml/min flow velocity, collecting efflux is
By the refined yam oligosaccharide mixing molasses of desalination.
3. a kind of preparation method of the yam oligosaccharide mixture of promotion proliferation of intestinal probiotics, it is characterised in that including following step
Suddenly：
(1) fresh Chinese yam clean stripping and slicing, add Chinese yam 8 times of weight of block clean water be beaten, be subsequently placed in homogenizer in
Homogeneous under 13MPa pressure, yam pulp is obtained, microjet processing, microjet are further carried out by microjet nanometer homogenizer
Treatment conditions are pressure 105MPa, 30 DEG C of temperature, obtain miniaturization yam pulp；
(2) 0.1M hydrochloric acid and 0.1MNa are used2CO3It is 6.00 to adjust above-mentioned miniaturization yam pulp pH value, is heated to 95 DEG C of holdings
10min is complete to the gelatinization of Chinese yam magma, and mesophilicα-diastase 2.2U/g is added when being cooled to 80 DEG C and is mixed, above-mentioned addition with
Dry meter in Chinese yam magma, it is 14.0% to be kept for 80 DEG C carry out within 30 minutes enzymolysis to slurries DE values for the first time, then rapid to rise
Temperature to 95 DEG C of enzyme deactivations in 3 minutes are lived, after the completion of be cooled to 50 DEG C, pH value is adjusted as 5.2 using 0.1M salt, while add fungi alphalise starch
Enzyme 10.0U/g and 1,4 beta-glucanase 0.9U/g carry out second and digested, and above-mentioned addition is with the yam pulp after digesting for the first time
Middle dry meter, second enzymolysis 3.5 hours to slurries DE values are 25.7%, are brought rapidly up to 98 DEG C, enzyme deactivation in 3 minutes living to obtain
Chinese yam malto-oligosaccharide and cellooligosaccharide mixing molasses crude product；
(3) by AB-8 macroreticular resins and above-mentioned Chinese yam malto-oligosaccharide and cellooligosaccharide mixing molasses crude product with 1：6 weight
Than well mixed, after concussion absorption 1.5hr, suction filtration obtains the Chinese yam malto-oligosaccharide and cellooligosaccharide mixed sugar by decolouring
Slurry；The above-mentioned mixing molasses by decolourizing are passed through into 001 × 7 cationic ion-exchange resin chromatographic column with 1.0ml/min flow velocity, received
It is to take off decationizing yam oligosaccharide mixing molasses to collect efflux；Decationizing yam oligosaccharide mixing molasses are taken off by above-mentioned
Anion therein is adsorbed by D201 anion exchange resin chromatographic column with 1.0ml/min flow velocity, collecting efflux is
By the refined yam oligosaccharide mixing molasses of desalination.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410740827.6A CN104531805B (en) | 2014-12-04 | 2014-12-04 | Promote the yam oligosaccharide mixture and preparation method of proliferation of intestinal probiotics |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201410740827.6A CN104531805B (en) | 2014-12-04 | 2014-12-04 | Promote the yam oligosaccharide mixture and preparation method of proliferation of intestinal probiotics |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| EP1897954A1 (en) * | 2006-09-11 | 2008-03-12 | Wageningen Universiteit | Methods and means for producing starch having at least one altered characteristic |
| CN102860359A (en) * | 2012-09-10 | 2013-01-09 | 广东省农业科学院农业生物技术研究所 | Black grain milk and steady-state preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1897954A1 (en) * | 2006-09-11 | 2008-03-12 | Wageningen Universiteit | Methods and means for producing starch having at least one altered characteristic |
| CN102860359A (en) * | 2012-09-10 | 2013-01-09 | 广东省农业科学院农业生物技术研究所 | Black grain milk and steady-state preparation method thereof |
Non-Patent Citations (2)
| Title |
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| "几种α-淀粉酶系生产麦芽低聚糖工艺研究";刘汉文等;《食品科技》;19911231(第6期);参见第32页左栏第2段-第33页右栏第1段，图1-3 * |
| "酶法提取山药中多糖的工艺研究";张元等;《中国中药杂志》;20080228;第33卷(第4期);第375页第1-4段，第375页右栏第1-4段 * |
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