CN104529773A - Preparation method and applications of lacti-glyceride caprylate feed additives - Google Patents
Preparation method and applications of lacti-glyceride caprylate feed additives Download PDFInfo
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- CN104529773A CN104529773A CN201410771025.1A CN201410771025A CN104529773A CN 104529773 A CN104529773 A CN 104529773A CN 201410771025 A CN201410771025 A CN 201410771025A CN 104529773 A CN104529773 A CN 104529773A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C67/00—Preparation of carboxylic acid esters
- C07C67/08—Preparation of carboxylic acid esters by reacting carboxylic acids or symmetrical anhydrides with the hydroxy or O-metal group of organic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C67/00—Preparation of carboxylic acid esters
- C07C67/48—Separation; Purification; Stabilisation; Use of additives
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C67/00—Preparation of carboxylic acid esters
- C07C67/48—Separation; Purification; Stabilisation; Use of additives
- C07C67/52—Separation; Purification; Stabilisation; Use of additives by change in the physical state, e.g. crystallisation
- C07C67/54—Separation; Purification; Stabilisation; Use of additives by change in the physical state, e.g. crystallisation by distillation
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Abstract
The invention discloses a preparation method and applications of lacti-glyceride caprylate feed additives. The method comprises the following steps: A, weighting a caprylic acid, glycerol and a lactic acid in molar ratio; B, under the protection of N2, adding a boric acid and glycerol into a reaction kettle; C, heating reflux, and reducing the temperature; D, sequentially adding a caprylic acid, hydrogenated resin, and cyclohexane into the reaction kettle again; E, gradually increasing the internal temperature of the reaction kettle, and maintaining a certain temperature for a time; F, cooling to room temperature, and adding 0.1 M of a hydrochloric acid to stir; G, after a solution is stabilized, opening a liquid outlet valve of the reaction kettle to remove a boric acid-hydrochloric acid mixture by separating and filtering; H, adding a lactic acid into the reaction kettle, and reacting at normal pressure; J, opening a knockout valve of the reaction kettle to separate a water phase; and K, carrying out reduced pressure distillation so as to recover cyclohexane, and separating hydrogenated resin, so that a lacti-glyceride caprylate feed additive is obtained. The method is simple in process, high in safety and low in cost, and has the advantages of promoting growth, improving the conversion efficiency of feeds, and effectively replacing an antibacterial growth promoter.
Description
Technical field
The invention belongs to herding or field of feed, more specifically relate to a kind of preparation method of sad glyceride lactate fodder additives, also relate to the purposes of a kind of sad glyceride lactate as fodder additives simultaneously.
Background technology
Feeding antibiotic has played vital role in animal health and husbandry sector.But due to the life-time service of these additives and abuse, its drawback is familiar with day by day widely: 1. microbiotic is residual in animal products meat, egg, milk, hair, skin, be detrimental to health, as produced " three cause ", irritated, even produce disease or potential hazard that the current scientific level of the mankind also fails to understand.2. cause the microorganism of livestock and poultry inside and outside to produce resistance or resistance to microbiotic, drug effect reduces, and dosage constantly increases, and also makes drug residue even more serious while of making aquaculture cost ever-increasing, forms vicious cycle.3., after livestock and poultry life-time service or abuse of antibiotics, while suppression pathogenic microorganism, also killed microorganism species useful normally in animal body, made body normal Tiny ecosystem system unbalance, cause animal autogenous infection or superinfection.4. make livestock and poultry produce microbiotic to rely on, suppress growing or reducing the immunologic function of animal of animal immune system, resistance against diseases is declined.
In order to overcome all drawbacks that antibiotic etc additive brings, the scientist of countries in the world makes great efforts to seek and develop the novel fodder additive had no side effect.Desirable Substitutes For Antibiotic generally has following characteristics: 1. material itself or its meta-bolites have no side effect to animal; 2. to environment without effect or act on little; 3. not easily or not bring out resistant organism to produce, also not easily bring out the generation of crossing drug resistant bacterium; 4. kill or suppress the growth and breeding of pathogenic micro-organism; 5. feed palatability is not affected.Current feeding antibiotic substitute is constantly weeded out the old and bring forth the new, and sums up, and current feeding antibiotic substitute mainly contains following 6 kinds: 1. souring agent; 2. zymin; 3. oligosaccharides; 4. herbal medicine and plant milk extract class; 5. probiotics; 6. antibacterial peptide.But these substitutes exist such as production technology imperfection, quality product to be in varying degrees difficult to ensure, to feed manufacturing conditional instability, destroy the problems such as feed Middle nutrition material.
In recent years, the bacteriostatic activity of lipid acid and derivative thereof receives the concern of people always.There are some researches show, lipid acid and derivative thereof can suppress filamentous fungus and saccharomycetic growth, the Chinese juniper shape mould easily contaminated in such as bread, Penicllium chrysogenum, monascus and common aspergillus niger, penicillum requeforti, penicillium jensenii, Candida albicans, yeast saccharomyces cerevisiae etc.; Simultaneously also can the growth and breeding of anti-bacteria, and gram-negative bacteria is better than to the restraining effect of gram-positive bacteria, but there is strain differences, as very weak to the restraining effect of the gram-negative bacterias such as intestinal bacteria, salmonella, pseudomonas, and to the gram-negative bacteria such as helicobacter pylori, Campylobacter, there is very strong restraining effect; Also report is had to other common restraining effect causing rotten malignant bacteria (Salmonella typhimurtum, listeria monocytogenes, Vibrio parahemolyticus, clostridium botulinum, meat bacillus, lactobacillus curvatus, lactobacillus sake, Pseudomonas fluorescens, liquefaction thunder Salmonella, heat dead circumfili bacterium, enterococcus faecalis, subtilis, bacillus cereus etc.).
Existing document shows, Capmul MCM C8 has anti-microbial effect, is used as sanitas and emulsifying agent, there is not yet the preparation of sad glyceride lactate and the report of anti-microbial effect, also has no its utilisation technology scheme as fodder additives.
Summary of the invention
The object of the invention is the preparation method that there are provided sad glyceride lactate fodder additives.This preparation method's technological line is reasonable, and technique is simple, the advantage that security is high, with low cost.
Another object of the present invention there are provided sad glyceride lactate as the application of fodder additives in livestock and poultry and aquatic animal feed, there is growth promoting effects, improve feed efficiency, reduce disease incident, effectively can substitute antivirus somatotropic agent.
In order to realize above-mentioned object, the present invention adopts following technical measures:
A preparation method for sad glyceride lactate fodder additives, its step is (having three kinds of products):
The first product: sad glyceride lactate refers to the sad lactyl-lactic acid glyceryl ester of list that and two molecule lactic acid sad by a part and a part glycerine esterification generate, and its molecular formula is C
17h
30o
8, molecular weight: 362.42, structural formula See Figure:
In figure, " H " represents hydrogen, " O " represents oxygen, " C " represents carbon, and footmark numeral 2,3,6 etc. represents number, and "-" represents singly-bound, and "=" represents double bond.
A. sad, the glycerol of 92 weight parts and the lactic acid of 220 weight parts of 144 weight parts is taken respectively.
B. at N
2under protection, in reactor, add the boric acid of 62 weight parts and the glycerol of 92 weight parts.
C.198-202 DEG C reflux 3-4 hour, reduces temperature room temperature (20-25 DEG C, identical below).
D. in reactor, the hydrogenated resin of sad, 10 weight parts of 144 weight parts is added again successively, the hexanaphthene of 200 parts by volume.
E. raise reactor temperature gradually to 148-152 DEG C, maintain 3-4 hour.
F. be cooled to room temperature, the 0.1M hydrochloric acid adding 100 weight parts stirs 58-62 minute.
G., after solution-stabilized, reactor fluid valve separating and filtering removing boric acid hydrochloric acid mixed solution is opened.
H. add in reactor by the lactic acid of 220 weight parts again, at normal pressure 128-132 DEG C, the reaction times is 4-5 hour.
I. open reactor skimmer valve and isolate aqueous phase.
Hexanaphthene is reclaimed in J, 0.5KPa underpressure distillation, isolates hydrogenated resin, namely obtains single sad lactyl-lactic acid glyceryl ester fodder additives.
A preparation method for sad glyceride lactate fodder additives, the steps include:
The second product: sad glyceride lactate is that its molecular formula is C by the sad sad single glyceride lactate of list generated with a part lactic acid and a part glycerine esterification of a part
14h
26o
6, molecular weight: 290.36, structural formula See Figure:
In figure, " H " represents hydrogen, " O " represents oxygen, " C " represents carbon, and footmark numeral 2,3,6 etc. represents number, and "-" represents singly-bound, and "=" represents double bond.
A. sad, the glycerol of 92 weight parts and the lactic acid of 110 weight parts of 144 weight parts is taken respectively.
B. first by the hexanaphthene of the glycerol of 92 weight parts, 200 parts by volume and the hydrogenated resin of 10 weight parts, reactor is added successively.
C. raise reactor temperature gradually to 148-152 DEG C, slowly add the sad of 144 weight parts.
D. open reactor skimmer valve and collect aquifer yield when reaching the water of 18 parts by volume, in reactor, slowly add the lactic acid of 110 weight parts.
E. at normal pressure 128-132 DEG C, the reaction times is 4-5 hour, the termination reaction when reactor skimmer valve collection aquifer yield reaches the water of 45 parts by volume.
Hexanaphthene is reclaimed in F, 0.5KPa underpressure distillation, isolates hydrogenated resin, obtains light yellow clear liquid and namely obtains single sad single glyceride lactate.
A preparation method for sad glyceride lactate fodder additives, the steps include:
The third product: sad glyceride lactate is that its molecular formula is C by the sad two sad single glyceride lactates generated with a part lactic acid and a part glycerine esterification of two molecules
22h
40o
7, molecular weight: 416.55, structural formula See Figure:
In figure, " H " represents hydrogen, " O " represents oxygen, " C " represents carbon, and footmark numeral 2,3,6 etc. represents number, and "-" represents singly-bound, and "=" represents double bond.
A. sad, the glycerol of 92 weight parts and the lactic acid of 110 weight parts of 288 weight parts is taken respectively.
B. at N
2under protection, in reactor, add the boric acid of 62 weight parts and the glycerol of 92 weight parts.
C.198-202 DEG C reflux 3 hours, reduces temperature to room temperature.
D. in reactor, the lactic acid of 110 weight parts, the hydrogenated resin of 10 weight parts is added again successively, the hexanaphthene of 200 parts by volume.
E. raise reactor temperature to 150 DEG C gradually, maintain 3-4 hour.
F. be cooled to room temperature, the 0.1M hydrochloric acid adding 100 weight parts stirs 1 hour.
G., after solution-stabilized, reactor fluid valve separating and filtering removing boric acid hydrochloric acid mixed solution is opened.
H. add in reactor by the sad of 288 weight parts again, at normal pressure 148-152 DEG C, the reaction times is 3-4 hour.
I. reactor skimmer valve is opened when isolating the water of 40 parts by volume, stopped reaction.
Hexanaphthene is reclaimed in J, 0.5KPa underpressure distillation, isolates hydrogenated resin, obtains yellow liquid i.e. two sad glyceride lactates.
The application of sad glyceride lactate fodder additives in livestock and poultry and aquatic animal feed, its step is (all having four kinds of modes):
First kind of way: directly addition manner
In the livestock and poultry that the sad glyceride lactate fodder additives taking 0.05 ~ 0.5 weight part joins 99.95 ~ 99.50 weight parts or aquatic animal feed, drop in mixing machine and fully mix, form the livestock and poultry containing sad glyceride lactate fodder additives or aquatic animal feed.
The weight sum of above-described sad glyceride lactate fodder additives and livestock and poultry or aquatic animal feed is 100.
The second way: pre-mixture addition manner
The carrier (being applicable to any one carrier of feed) of A, the sad glyceride lactate fodder additives taking 1 weight part and 1 weight part, fully mixes through mixing machine, defines containing sad glyceride lactate fodder additives pre-mixture.
In B, the livestock and poultry joining 99.9 ~ 99.0 weight parts containing sad glyceride lactate fodder additives pre-mixture taking 0.1 ~ 1 weight part and aquatic animal feed, drop in mixing machine and fully mix, form the livestock and poultry containing sad glyceride lactate fodder additives pre-mixture and aquatic animal feed.
The above-described weight sum containing sad glyceride lactate fodder additives pre-mixture and livestock and poultry or aquatic animal feed is 100.
The third mode: Preblend addition manner is example with 1% (accounting for the ratio of perfect compound feed, identical below) livestock and poultry or aquatic animal Preblend.
In 1% livestock and poultry that A, the sad glyceride lactate fodder additives taking 5 ~ 50 weight parts join 95 ~ 50 weight parts or aquatic animal Preblend, fully mix through mixing machine, define 1% livestock and poultry containing sad glyceride lactate fodder additives or aquatic animal Preblend.
The weight sum of above-described sad glyceride lactate fodder additives and 1% livestock and poultry or aquatic animal Preblend is 100.
Described Preblend is the abbreviation of additive premix, and it is by one or more minor components and diluent or carrier proportioning on request, the osculant compound feed products made after Homogeneous phase mixing.Preblend is a kind of important component of perfect compound feed, then mixes a certain proportion of protein feed (fish meal, dregs of beans etc.), energy feed (corn, Chinese sorghum, barley etc.), mineral substance (stone flour, secondary calcium phosphate etc.) etc. and just become the complete feed meeting Animal nutrition needs.General ratio shared in perfect compound feed is 0.01% ~ 10%.Preblend composition: containing following material in its raw material: trace element, comprises copper sulfate, manganous sulfate, zinc sulfate, ferrous sulfate, potassiumiodide, Sodium Selenite etc.; Amino acid, antioxidant, microbiotic and multivitamin etc.It is that livestock and poultry life physically well develops soon, meat is good, the required trophic level that is of high nutritive value calculates, and adopts modern processing units according to making, and is fully mixed by above raw material and makes.This Preblend can raise unit as an independent hay varieties supply, and user mixes other feedstuff raw materials such as protein feed, energy feed, mineral substance by a certain percentage, namely becomes mixed feed after stirring.
In B, the livestock and poultry joining 99 weight parts containing 1% livestock and poultry of sad glyceride lactate fodder additives or aquatic animal Preblend taking 1 weight part or aquatic animal feed, drop in mixing machine and fully mix, form the livestock and poultry containing sad glyceride lactate fodder additives or aquatic animal feed.
Above-described is 100 containing the Preblend of sad glyceride lactate fodder additives and the weight sum of livestock and poultry or aquatic animal feed.
4th kind of mode: concentrate feed addition manner, for 40% livestock and poultry or aquatic animal concentrate feed.
In 40% livestock and poultry that A, the sad glyceride lactate fodder additives taking 0.75 ~ 7.5 weight part join 99.25 ~ 92.5 weight parts or aquatic animal concentrated fodder, drop in mixing machine and fully mix, form the livestock and poultry containing sad glyceride lactate fodder additives and aquatic animal concentrated fodder.
The weight sum of above-described sad glyceride lactate fodder additives and 40% livestock and poultry or aquatic animal concentrated fodder is 100.
B, by 40% livestock and poultry or aquatic animal concentrate feed according to the method in its working instructions, form the livestock and poultry containing sad glyceride lactate fodder additives and aquatic animal feed.
Described concentrated fodder, is also called protein supplementaling feed, is by the formulated mixed feed work in-process of protein feed (fish meal, soya-bean cake etc.), mineral feed (bone meal stone flour etc.) and additive premix.Mix a certain proportion of energy feed (corn, Chinese sorghum, barley etc.) again and just become the complete feed meeting Animal nutrition needs, there is the advantages such as protein content high (generally between 30% ~ 50%), nutritive ingredient be comprehensive, easy to use.General ratio shared in perfect compound feed is 20% ~ 40%.Concentrated fodder composition: containing following material in its raw material: mineral substance, comprises bone meal, stone flour (calcium powder) or oyster shell whiting; Trace element, comprises copper sulfate, manganous sulfate, zinc sulfate, ferrous sulfate, potassiumiodide, Sodium Selenite etc.; Amino acid, antioxidant, microbiotic, protein feed and multivitamin etc.It is that livestock and poultry life physically well develops soon, meat is good, the required trophic level that is of high nutritive value calculates, and adopts modern processing units according to making, and is fully mixed by above raw material and makes.This concentrated fodder can raise unit as an independent hay varieties supply, and user mixes energy feed by a certain percentage, namely becomes mixed feed after stirring.Such as: the proportioning of layer chicken feed is 30% laying hen concentrated fodder, the energy feed of 70%; The proportioning of pig feed is 20% concentrated fodder, the energy feed of 80%.The most applicable rural households exclusively engaging in a particular productionor trade of concentrated fodder is raised pigs, use of raising chickens.The grain utilizing oneself to produce and byproduct, then be equipped with concentrated fodder, get final product Direct-fed, decrease unnecessary transit link, abridged edition saving of labor.
Described livestock and poultry are specially pig, ox, sheep, chicken, duck, goose any one wherein.
Described aquatic animal is specially the freshwater aquiculture animal any one wherein such as green grass or young crops, grass, silver carp, flathead, tilapia, hybridized prussian carp, crucian, shrimp, crab.
Outstanding advantages of the present invention is: sad glyceride lactate effectively can suppress animal intestinal harmful microbe to be bred, promote animal health, reduces and reduces disease incident more than 44%, improves day weight gain more than 9.5%, and then improves breeding performonce fo animals.This fodder additives has the advantage that security is high, have no drug resistance, and effectively can substitute feeding antibiotic, meet healthy aquaculture developing direction.
Embodiment
Embodiment 1:
A preparation method for sad glyceride lactate fodder additives, the steps include:
1. take that 144g is sad, 92g glycerol, 220g lactic acid, 62g boric acid, 10g hydrogenated resin and 200ml hexanaphthene respectively.
2. at N
2under protection, in the reactor of 1L, add boric acid and glycerol.
3.200 DEG C of reflux 3 hours, reduce temperature to room temperature (20-25 DEG C).
4. in reactor, add sad, hydrogenated resin successively again, hexanaphthene.
5. raise reactor temperature gradually, maintain 150 DEG C, 3 hours.
6. be cooled to room temperature, the 0.1M hydrochloric acid adding 100g stirs 1 hour.
7., after solution-stabilized, open reactor fluid valve separating and filtering removing boric acid hydrochloric acid mixed solution.
8. add in reactor by lactic acid again, at normal pressure 130 DEG C, the reaction times is 4 hours.
9. open reactor skimmer valve and isolate aqueous phase.
10.0.5KPa hexanaphthene is reclaimed in underpressure distillation, isolates hydrogenated resin, namely obtains single sad lactyl-lactic acid glyceryl ester.
Embodiment 2:
A preparation method for sad glyceride lactate fodder additives, the steps include:
1. take that 144g is sad, 92g glycerol, 110g lactic acid, 62g boric acid, 10g hydrogenated resin and 200ml hexanaphthene respectively.
2., by glycerol, hexanaphthene and hydrogenated resin, add 1L reactor successively.
3. raise reactor temperature to 150 DEG C gradually, it is sad slowly to add.
4. open reactor skimmer valve and collect aquifer yield when reaching the water of 18ml, slowly in reactor, add lactic acid.
5. at normal pressure 130 DEG C, the reaction times is 4 hours, the termination reaction when reactor skimmer valve collection aquifer yield reaches 45ml water.
6.0.5KPa hexanaphthene is reclaimed in underpressure distillation, isolates hydrogenated resin, obtains light yellow clear liquid and namely obtains single sad single glyceride lactate.
Embodiment 3:
A preparation method for sad glyceride lactate fodder additives, the steps include:
1. take that 288g is sad, 92g glycerol, 110g lactic acid, 62g boric acid, 10g hydrogenated resin and 200ml hexanaphthene respectively.
2. at N
2under protection, in 1L reactor, add boric acid and glycerol successively.
3.200 DEG C of reflux 3 hours, reduce temperature to room temperature (20-25 DEG C).
4. in reactor, add lactic acid, hydrogenated resin and hexanaphthene successively again.
5. raise reactor temperature gradually, maintain 150 DEG C, 3 hours.
6. be cooled to room temperature, the 0.1M hydrochloric acid adding 100g stirs 1 hour.
7., after solution-stabilized, open reactor fluid valve separating and filtering removing boric acid hydrochloric acid mixed solution.
8. add in reactor by sad again, at normal pressure 150 DEG C, the reaction times is 3 hours.
9. open reactor skimmer valve when isolating 40ml water, stopped reaction.
10.0.5KPa hexanaphthene is reclaimed in underpressure distillation, isolates hydrogenated resin, obtains yellow liquid i.e. two sad single glyceride lactates.
Embodiment 4:
Single sad lactyl-lactic acid glyceryl ester, single sad single glyceride lactate and two sad single glyceride lactates are tested according to following table arrangement, measures the bacteriostatic activity of sad lactate with Odontothrips loti.
Table 1 sad lactate In Vitro Bacteriostasis effect (mm)
The data of table 1 show, sad lactate has restraining effect to intestinal bacteria and Salmonellas, and the size of its In Vitro Bacteriostasis circle is respectively 13.47 ~ 18.29mm and 11.70 ~ 15.91mm.
Embodiment 5:
The application of sad glyceride lactate fodder additives in weanling pig feed, the steps include:
1, the sad lactyl-lactic acid glyceryl ester of list taking 50 weight parts respectively drops in mixing machine with the defatted rice bran of 50 weight parts and mixes, and namely prepares sad glyceride lactate fodder additives pre-mixture I.
2, the sad lactate fodder additives pre-mixture I taking 0.1 weight part respectively drops in mixing machine with the weanling pig feed of 99.9 weight parts and mixes, and namely prepares the weanling pig feed containing sad glyceride lactate fodder additives I.
3, the sad single glyceride lactate of list taking 50 weight parts respectively drops in mixing machine with the defatted rice bran of 50 weight parts and mixes, and namely prepares sad glyceride lactate fodder additives pre-mixture II.
4, the sad glyceride lactate fodder additives pre-mixture II taking 0.18 weight part respectively drops in mixing machine with the weanling pig feed of 99.82 weight parts and mixes, and namely prepares the weanling pig feed containing sad glyceride lactate fodder additives II.
5, the two sad single glyceride lactates taking 50 weight parts respectively drop in mixing machine with the defatted rice bran of 50 weight parts and mix, and namely prepare sad glyceride lactate fodder additives pre-mixture III.
6, the sad glyceride lactate fodder additives pre-mixture III taking 0.5 weight part respectively drops in mixing machine with the weanling pig feed of 99.5 weight parts and mixes, and namely prepares the weanling pig feed containing sad glyceride lactate fodder additives III.
7, select the DLY weanling pig that the body weight of the disposable wean of 96 21 ages in days is consistent with sex, be divided into 4 groups at random, be i.e. control group and test I, II, III group, every component 3 repetition (hurdle), 8, every hurdle.
8, four groups basal diet trophic level of feeding is consistent.Wherein test that I, II, III group is fed containing sad lactate fodder additives I respectively, containing sad lactate fodder additives II, weanling pig feed containing sad lactate fodder additives III, control group fed adds 0.04% kangdisu sulfas pre-mixture (kangdisu sulfas content is 10%).
9, feed weanling pig 21 days, result is as following table 2:
Table 2 adds sad glyceride lactate fodder additives to the impact of Growth Performance of Weaning Piglets
Note: the female different person of colleague's shoulder marking-up represents significant difference (p<0.05).Below roughly the same.
Result shows, applies fodder additives of the present invention, compared with adding the control group of kangdisu sulfas, can significantly improve piglet day weight gain more than 14.62%; Reduce feed-weight ratio more than 9.48%; Reduce Incidence of Diarrhea 43.66%.Therefore, sad glyceride lactate fodder additives can promote piglet growth, improves feed efficiency, reduce disease incident, effectively can substitute antivirus somatotropic agent.This embodiment is applicable to pig, ox, sheep, rabbit any one wherein.
Embodiment 6:
Sad glyceride lactate fodder additives, preparing the application in hybridized prussian carp feed, the steps include:
1, the sad lactyl-lactic acid glyceryl ester of list taking 50 weight parts respectively drops in mixing machine with the defatted rice bran of 50 weight parts and mixes, and namely prepares sad glyceride lactate fodder additives pre-mixture I.
2, the sad lactate fodder additives pre-mixture I taking 0.2 weight part respectively drops in mixing machine with the hybridized prussian carp feed of 99.8 weight parts and mixes, and namely prepares the hybridized prussian carp feed containing sad glyceride lactate fodder additives I.
3, the sad single glyceride lactate of list taking 50 weight parts respectively drops in mixing machine with the defatted rice bran of 50 weight parts and mixes, and namely prepares sad glyceride lactate fodder additives pre-mixture II.
4, the sad lactate fodder additives pre-mixture II taking 0.38 weight part respectively drops in mixing machine with the hybridized prussian carp feed of 99.62 weight parts and mixes, and namely prepares the hybridized prussian carp feed containing sad glyceride lactate fodder additives II.
5, the two sad single glyceride lactates taking 50 weight parts respectively drop in mixing machine with the defatted rice bran of 50 weight parts and mix, and namely prepare sad glyceride lactate fodder additives pre-mixture III.
6, the sad lactate fodder additives pre-mixture III taking 1 weight part respectively drops in mixing machine with the hybridized prussian carp feed of 99 weight parts and mixes, and namely prepares the hybridized prussian carp feed containing sad glyceride lactate fodder additives III.
7, select body weight to be hybridized prussian carp 600 tail of 9.27 ± 0.06g, be divided into 4 groups at random, be i.e. control group and test I, II, III group, every component 3 repetition (case), every case 50 tail.
8, each group of basal diet trophic level of feeding is consistent.Test I, II, III group to feed containing sad glyceride lactate fodder additives I, containing sad glyceride lactate fodder additives II, hybridized prussian carp feed containing sad glyceride lactate fodder additives III, control group fed adds 2mg/kg bambermycin.
9, by the growth test of 8 weeks, sad glyceride lactate fodder additives is evaluated as the impact of antivirus somatotropic agent on allogynogenetic crucian carp performance.The results are shown in following table 3.
Table 3 adds sad glyceride lactate fodder additives to the impact of crucian growth performance
| Project | Blank group | Test I group | Test II group | Test III group |
| Specific growth rate % | 3.66±0.12 b | 4.27±0.04 a | 4.38±0.09 a | 4.32±0.04 a |
| Feed coefficient | 1.49±0.05 b | 1.31±0.03 a | 1.32±0.06 a | 1.34±0.02 a |
| Survival rate % | 98.10±2.00 | 98.70±2.07 | 99.30±1.09 | 99.50±1.10 |
Test-results shows, applies fodder additives of the present invention, compared with adding the control group of bambermycin, can improve crucian specific growth rate 16.67%, feed coefficient reduces by 10.06%.Therefore, sad glyceride lactate fodder additives is by improving crucian serum resistance of oxidation, thus growth promoting effects, raising feed efficiency, effectively can substitute antivirus somatotropic agent.This embodiment is applicable to the freshwater aquiculture animal any one wherein such as green grass or young crops, grass, silver carp, flathead, tilapia, hybridized prussian carp, crucian, shrimp, crab.
Claims (4)
1. a preparation method for sad glyceride lactate fodder additives, is characterized in that: the sad glyceride lactate sad lactyl-lactic acid glyceryl ester of list that sad and two molecule lactic acid and a part glycerine esterification generate by a part, its molecular formula is C
17h
30o
8, molecular weight: 362.42, the steps include:
A, sad, the glycerol of 92 weight parts taking 144 weight parts respectively and the lactic acid of 220 weight parts;
B, at N
2under protection, in reactor, add the boric acid of 62 weight parts and the glycerol of 92 weight parts;
C, 198-202 DEG C of reflux 3-4 hour, reduces temperature room temperature;
D, in reactor, add the hydrogenated resin of sad, 10 weight parts of 144 weight parts more successively, the hexanaphthene of 200 parts by volume;
E, gradually rising reactor temperature, to 148-152 DEG C, maintain 3-4 hour;
F, be cooled to room temperature, the 0.1M hydrochloric acid adding 100 weight parts stirs 58-62 minute;
G, after solution-stabilized, open reactor fluid valve separating and filtering removing boric acid hydrochloric acid mixed solution;
H, add in reactor by the lactic acid of 220 weight parts again, at normal pressure 128-132 DEG C, the reaction times is 4-5 hour;
J, open reactor skimmer valve and isolate aqueous phase;
Hexanaphthene is reclaimed in K, 0.5KPa underpressure distillation, isolates hydrogenated resin, obtains single sad lactyl-lactic acid glyceryl ester fodder additives.
2. the preparation method of a kind of sad glyceride lactate fodder additives according to claim 1, it is characterized in that: sad glyceride lactate is that its molecular formula is C by the sad sad single glyceride lactate of list generated with a part lactic acid and a part glycerine esterification of a part
14h
26o
6, molecular weight: 290.36, the steps include:
A, sad, the glycerol of 92 weight parts taking 144 weight parts respectively and the lactic acid of 110 weight parts;
B, first by the hexanaphthene of the glycerol of 92 weight parts, 200 parts by volume and the hydrogenated resin of 10 weight parts, add reactor successively;
C, gradually rising reactor temperature, to 148-152 DEG C, slowly add the sad of 144 weight parts;
D, open reactor skimmer valve and collect aquifer yield when reaching the water of 18 parts by volume, in reactor, slowly add the lactic acid of 110 weight parts;
E, at normal pressure 128-132 DEG C the reaction times be 4-5 hour, when reactor skimmer valve collects termination reaction when aquifer yield reaches the water of 45 parts by volume;
Hexanaphthene is reclaimed in F, 0.5KPa underpressure distillation, isolates hydrogenated resin, obtains light yellow clear liquid and namely obtains single sad single glyceride lactate;
The preparation method of a kind of sad glyceride lactate fodder additives according to claim 1, is characterized in that: sad glyceride lactate is that its molecular formula is C by the sad two sad single glyceride lactates generated with a part lactic acid and a part glycerine esterification of two molecules
22h
40o
7, molecular weight: 416.55, the steps include:
A, sad, the glycerol of 92 weight parts taking 288 weight parts respectively and the lactic acid of 110 weight parts;
B, at N
2under protection, in reactor, add the boric acid of 62 weight parts and the glycerol of 92 weight parts;
C, 198-202 DEG C of reflux 3 hours, reduces temperature to room temperature;
D, in reactor, add the lactic acid of 110 weight parts, the hydrogenated resin of 10 weight parts more successively, the hexanaphthene of 200 parts by volume;
E, gradually rising reactor temperature to 150 DEG C, maintain 3-4 hour;
F, be cooled to room temperature, the 0.1M hydrochloric acid adding 100 weight parts stirs 1 hour;
G, after solution-stabilized, open reactor fluid valve separating and filtering removing boric acid hydrochloric acid mixed solution;
H, add in reactor by the sad of 288 weight parts again, at normal pressure 148-152 DEG C, the reaction times is 3-4 hour;
J, open reactor skimmer valve when isolating the water of 40 parts by volume, stopped reaction;
Hexanaphthene is reclaimed in K, 0.5KPa underpressure distillation, isolates hydrogenated resin, obtains yellow liquid i.e. two sad glyceride lactates.
3. a kind of sad glyceride lactate fodder additives application in animal and fowl fodder of claims 1 to 3 described in any one;
Described livestock and poultry are pig, ox, sheep, chicken, duck, goose any one wherein.
4. a kind of sad glyceride lactate fodder additives application in aquatic animal feed of claims 1 to 3 described in any one; Described aquatic animal is green grass or young crops, grass, silver carp, flathead, tilapia, hybridized prussian carp, crucian, shrimp, crab freshwater aquiculture animal any one wherein.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110122667A (en) * | 2019-05-30 | 2019-08-16 | 河南正通化工有限公司 | A kind of feed antibacterial agent and preparation method thereof |
| WO2023137659A1 (en) * | 2022-01-20 | 2023-07-27 | 南京泛太化工医药研究所 | Series of polymers having properties of acid-sensitive degradation and temperature sensitivity and drug loading composition thereof |
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| CN107927350A (en) * | 2017-12-29 | 2018-04-20 | 湖北浩华生物技术有限公司 | Purposes, feed addictive and feed of the valerin on feed addictive is prepared |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103082094A (en) * | 2013-01-31 | 2013-05-08 | 武汉工业学院 | Application of butyrin lactate as feed additive |
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| CN103082094A (en) * | 2013-01-31 | 2013-05-08 | 武汉工业学院 | Application of butyrin lactate as feed additive |
Non-Patent Citations (1)
| Title |
|---|
| 未本美等: "单辛酸甘油酯的合成及抗菌活性的初步研究", 《食品科技》, no. 9, 31 December 2007 (2007-12-31), pages 163 - 164 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110122667A (en) * | 2019-05-30 | 2019-08-16 | 河南正通化工有限公司 | A kind of feed antibacterial agent and preparation method thereof |
| WO2023137659A1 (en) * | 2022-01-20 | 2023-07-27 | 南京泛太化工医药研究所 | Series of polymers having properties of acid-sensitive degradation and temperature sensitivity and drug loading composition thereof |
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Effective date of registration: 20181130 Address after: 437199 Xianning High-tech Industrial Park, Xianning City, Hubei Province Patentee after: HUBEI HAOHUA BIOTECHNOLOGY CO., LTD. Address before: 430023 No. 68 Xuefu South Road, Changqing Garden, Hankou, Wuhan, Hubei. Co-patentee before: HUBEI HAOHUA BIOTECHNOLOGY CO., LTD. Patentee before: Wuhan Polytechnic University |