CN104529773B - A kind of preparation method and application of sad glyceride lactate feed additive - Google Patents
A kind of preparation method and application of sad glyceride lactate feed additive Download PDFInfo
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- CN104529773B CN104529773B CN201410771025.1A CN201410771025A CN104529773B CN 104529773 B CN104529773 B CN 104529773B CN 201410771025 A CN201410771025 A CN 201410771025A CN 104529773 B CN104529773 B CN 104529773B
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C67/00—Preparation of carboxylic acid esters
- C07C67/08—Preparation of carboxylic acid esters by reacting carboxylic acids or symmetrical anhydrides with the hydroxy or O-metal group of organic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C67/00—Preparation of carboxylic acid esters
- C07C67/48—Separation; Purification; Stabilisation; Use of additives
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C67/00—Preparation of carboxylic acid esters
- C07C67/48—Separation; Purification; Stabilisation; Use of additives
- C07C67/52—Separation; Purification; Stabilisation; Use of additives by change in the physical state, e.g. crystallisation
- C07C67/54—Separation; Purification; Stabilisation; Use of additives by change in the physical state, e.g. crystallisation by distillation
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Abstract
The invention discloses the preparation method and application of a kind of sad glyceride lactate feed additive, step: A, weigh by the mol ratio of sad, glycerol and lactic acid; B, at N2Under protection, in reactor, add boric acid and glycerol; C, it is heated to reflux, reduces temperature; D, in reactor, it is sequentially added into sad, hydrogenated resin, hexamethylene again; E, gradually rise reactor temperature, maintain uniform temperature and time; F, it is cooled to room temperature, adds the stirring of 0.1M hydrochloric acid; G, after solution-stabilized, open reactor liquid valve door separating and filtering remove boric acid hydrochloric acid mixed solution; H, again lactic acid is added in reactor, react at ambient pressure; J, open reactor liquor separator valve and isolate aqueous phase; K, decompression Distillation recovery hexamethylene, isolate hydrogenated resin, obtains single sad lactyl-lactic acid glyceride feed additive. Technique is simple, and safety is high, with low cost, has promotion growth, improves feed efficiency, effectively substitutes antivirus somatotropic agent.
Description
Technical field
The invention belongs to herding or field of feed, the preparation method being more particularly to a kind of sad glyceride lactate feed additive, also relate to a kind of sad glyceride lactate purposes as feed additive.
Background technology
Feeding antibiotic has played important function in animal health and husbandry sector. But due to the life-time service of these additives and abuse, its drawback is recognized day by day widely: 1. antibiotic remains in animal products meat, egg, milk, hair, skin, it is detrimental to health, as produced " three cause ", allergy, even produce disease or potential hazard that the current scientific level of the mankind also fails to understand. Antibiotic is produced drug resistance or Drug resistance by the microorganism 2. causing poultry inside and outside, and drug effect reduces, and dosage is continuously increased, and also makes drug residue even more serious while of making aquaculture cost ever-increasing, forms vicious cycle. 3., after poultry life-time service or abuse of antibiotics, while suppressing pathogenic microorganism, also killed microorganism species useful normally in animal body, made body normal Tiny ecosystem system unbalance, cause animal autogenous infection or superinfection. 4. make poultry produce to rely on to antibiotic, it is suppressed that the growth promoter of animal immune system or reduce the immunologic function of animal, make resistance against diseases decline.
For all drawbacks overcoming antibiotic etc additive to bring, the scientist of countries in the world makes great efforts to seek and develop the novel fodder additive having no side effect. Desirable Substitutes For Antibiotic generally has the following characteristics that 1. animal is had no side effect by material itself or its metabolite;2. environment without effect or is acted on little; 3. not easily or not bring out fastbacteria to produce, be not easy to bring out the generation of crossing drug resistant bacterium; 4. kill or suppress the growth and breeding of pathogenic microorganism; 5. feedstuff palatability is not affected. Current feeding antibiotic succedaneum is constantly weeded out the old and bring forth the new, and sums up, and current feeding antibiotic succedaneum mainly has following 6 kinds: 1. acidulant; 2. enzyme preparation; 3. oligosaccharides; 4. Chinese herbal medicine and plant extract class; 5. microbial ecological agent; 6. antibacterial peptide. But these succedaneum exist in varying degrees such as production technology imperfection, product quality be difficult to ensure that, to feed manufacturing conditional instability, destroy the problem such as feedstuff Middle nutrition material.
In recent years, the bacteriostatic activity of fatty acid and derivant thereof is constantly subjected to the concern of people. There are some researches show, fatty acid and derivant thereof can suppress filamentous fungi and saccharomycetic growth, Chinese juniper shape penicillium sp, Penicllium chrysogenum, monascus and common aspergillus niger, penicillium roqueforti, penicillium jensenii, Candida albicans, the saccharomyces cerevisiae etc. easily contaminated in such as bread; Also can suppress the growth and breeding of antibacterial simultaneously, and the inhibitory action of gram-positive bacteria is better than gram-negative bacteria, but there is strain differences, as the inhibitory action of the gram-negative bacterias such as escherichia coli, salmonella, pseudomonas is very weak, and the gram-negative bacteria such as helicobacter pylori, Campylobacter is had very strong inhibitory action; Other common inhibitory action causing rotten malignant bacteria (Salmonella typhimurtum, listeria monocytogenes, vibrio parahaemolyticus, Clostridium botulinum, meat bacillus, lactobacillus curvatus, Lactobacillus saki, pseudomonas fluorescens, liquefaction thunder Salmonella, heat dead circumfili bacterium, enterococcus faecalis, bacillus subtilis, bacillus cereus etc.) are also had been reported that.
Existing document shows, Capmul MCM C8 has antibacterial action, is used as preservative and emulsifying agent, there is not yet the report of the preparation of sad glyceride lactate and antibacterial action, also has no its application technology scheme as feed additive.
Summary of the invention
It is an object of the invention to the preparation method that there are provided sad glyceride lactate feed additive. This preparation method technology path is reasonable, and technique is simple, the advantage that safety is high, with low cost.
Another object of the present invention is to there are provided sad glyceride lactate as feed additive application in poultry and aquatic animal feed, there is promotion growth, improve feed efficiency, reduce disease incident, it is possible to effectively substitute antivirus somatotropic agent.
In order to realize above-mentioned purpose, the present invention adopts techniques below measure:
A kind of preparation method of sad glyceride lactate feed additive, its step is (having three kinds of products):
The first product: sad glyceride lactate refers to the sad lactyl-lactic acid glyceride of list that and two molecule lactic acid sad by a part generate with a part glycerine esterification, and its molecular formula is C17H30O8, molecular weight: 362.42, structural formula See Figure:
In figure, " H " represents hydrogen, " O " represents oxygen, " C " represents carbon, and footmark numeral 2,3,6 etc. represents number, and " " represents singly-bound, "=" represent double bond.
A. the glycerol of sad, 92 weight portions of 144 weight portions and the lactic acid of 220 weight portions are weighed respectively.
B. at N2Under protection, in reactor, add the boric acid of 62 weight portions and the glycerol of 92 weight portions.
C.198-202 DEG C it is heated to reflux 3-4 hour, reduces temperature room temperature (20-25 DEG C, identical below).
D. in reactor, it is sequentially added into the hydrogenated resin of sad, 10 weight portions of 144 weight portions, the hexamethylene of 200 parts by volume again.
E. gradually rise reactor temperature to 148-152 DEG C, maintain 3-4 hour.
F. being cooled to room temperature, the 0.1M hydrochloric acid adding 100 weight portions stirs 58-62 minute.
G., after solution-stabilized, open reactor liquid valve door separating and filtering and remove boric acid hydrochloric acid mixed solution.
H. being added in reactor by the lactic acid of 220 weight portions, at normal pressure 128-132 DEG C, the response time is 4-5 hour again.
I. open reactor liquor separator valve and isolate aqueous phase.
J, 0.5KPa reduce pressure Distillation recovery hexamethylene, isolate hydrogenated resin, namely obtain single sad lactyl-lactic acid glyceride feed additive.
The preparation method of a kind of sad glyceride lactate feed additive, the steps include:
The second product: sad glyceride lactate is sad by a part and the sad single glyceride lactate of list of a part lactic acid and the generation of a part glycerine esterification, and its molecular formula is C14H26O6, molecular weight: 290.36, structural formula See Figure:
In figure, " H " represents hydrogen, " O " represents oxygen, " C " represents carbon, and footmark numeral 2,3,6 etc. represents number, and " " represents singly-bound, "=" represent double bond.
A. the glycerol of sad, 92 weight portions of 144 weight portions and the lactic acid of 110 weight portions are weighed respectively.
B. first by the hydrogenated resin of the glycerol of 92 weight portions, the hexamethylene of 200 parts by volume and 10 weight portions, it is sequentially added into reactor.
C. gradually rise reactor temperature to 148-152 DEG C, be slowly added to the sad of 144 weight portions.
When D. opening the water that reactor liquor separator valve collection water yield reaches 18 parts by volume, in reactor, slowly add the lactic acid of 110 weight portions.
E. at normal pressure 128-132 DEG C, the response time is 4-5 hour, terminates reaction when reactor liquor separator valve collection water yield reaches the water of 45 parts by volume.
F, 0.5KPa reduce pressure Distillation recovery hexamethylene, isolate hydrogenated resin, obtain light yellow clear liquid and namely obtain single sad single glyceride lactate.
The preparation method of a kind of sad glyceride lactate feed additive, the steps include:
The third product: sad glyceride lactate is sad by two molecules and two sad single glyceride lactates of a part lactic acid and the generation of a part glycerine esterification, and its molecular formula is C22H40O7, molecular weight: 416.55, structural formula See Figure:
In figure, " H " represents hydrogen, " O " represents oxygen, " C " represents carbon, and footmark numeral 2,3,6 etc. represents number, and " " represents singly-bound, "=" represent double bond.
A. the glycerol of sad, 92 weight portions of 288 weight portions and the lactic acid of 110 weight portions are weighed respectively.
B. at N2Under protection, in reactor, add the boric acid of 62 weight portions and the glycerol of 92 weight portions.
C.198-202 DEG C it is heated to reflux 3 hours, reduces temperature to room temperature.
D. in reactor, it is sequentially added into the hydrogenated resin of the lactic acid of 110 weight portions, 10 weight portions, the hexamethylene of 200 parts by volume again.
E. gradually rise reactor temperature to 150 DEG C, maintain 3-4 hour.
F. being cooled to room temperature, the 0.1M hydrochloric acid adding 100 weight portions stirs 1 hour.
G., after solution-stabilized, open reactor liquid valve door separating and filtering and remove boric acid hydrochloric acid mixed solution.
H. again by the sad addition reactor of 288 weight portions, at normal pressure 148-152 DEG C, the response time is 3-4 hour.
When I. opening the water that reactor liquor separator valve isolates 40 parts by volume, stopped reaction.
J, 0.5KPa reduce pressure Distillation recovery hexamethylene, isolate hydrogenated resin, obtain the sad glyceride lactate of yellow liquid that is two.
The application in poultry and aquatic animal feed of a kind of sad glyceride lactate feed additive, its step is (all having four kinds of modes):
First kind of way: directly addition manner
Weigh in poultry or the aquatic animal feed that the sad glyceride lactate feed additive of 0.05～0.5 weight portion joins 99.95～99.50 weight portions, put in mixer and be sufficiently mixed, form the poultry containing sad glyceride lactate feed additive or aquatic animal feed.
The weight sum of above-described sad glyceride lactate feed additive and poultry or aquatic animal feed is 100.
The second way: pre-mixing agent addition manner
The carrier (any one carrier suitable in feedstuff) of A, the sad glyceride lactate feed additive weighing 1 weight portion and 1 weight portion, blended machine is sufficiently mixed, and defines containing sad glyceride lactate feed additive pre-mixing agent.
B, weigh in the poultry and the aquatic animal feed that join 99.9～99.0 weight portions containing sad glyceride lactate feed additive pre-mixing agent of 0.1～1 weight portion, put in mixer and be sufficiently mixed, form the poultry containing sad glyceride lactate feed additive pre-mixing agent and aquatic animal feed.
The above-described weight sum containing sad glyceride lactate feed additive pre-mixing agent with poultry or aquatic animal feed is 100.
The third mode: premix material addition manner, is example with 1% (accounting for the ratio of perfect compound feed, identical below) poultry or aquatic animal premix material.
A, weigh in 1% poultry or the aquatic animal premix material that the sad glyceride lactate feed additive of 5～50 weight portions joins 95～50 weight portions, blended machine is sufficiently mixed, and defines 1% poultry containing sad glyceride lactate feed additive or aquatic animal premix material.
The weight sum of above-described sad glyceride lactate feed additive and 1% poultry or aquatic animal premix material is 100.
Described premix material is the abbreviation of additive premix, and it is by one or more microcomponents and diluent or carrier proportioning on request, the middle type compound feed products made after Homogeneous phase mixing. Premix material is a kind of important component of perfect compound feed, then mixes a certain proportion of protein feeds (fish flour, bean cake etc.), energy feed (Semen Maydis, Sorghum vulgare Pers., Fructus Hordei Vulgaris etc.), mineral (stone powder, calcium hydrogen phosphate etc.) etc. and just become the complete feed meeting Animal nutrition needs. Being typically in ratio shared in perfect compound feed is 0.01%～10%. Premix material composition: containing following material in its raw material: trace element, including copper sulfate, manganese sulfate, zinc sulfate, ferrous sulfate, potassium iodide, sodium selenite etc.; Aminoacid, antioxidant, antibiotic and multivitamin etc. It is that poultry life physically well develops soon, meat is good, the required trophic level that is of high nutritive value is calculated according to making, and adopts modern process equipment, is sufficiently mixed by above raw material and makes. This premix material can raise unit as an independent hay varieties supply, and user mixes other feedstuffs such as protein feeds, energy feed, mineral by a certain percentage, namely becomes mixed feed after stirring.
B, 1% poultry containing sad glyceride lactate feed additive weighing 1 weight portion or aquatic animal premix material join in poultry or the aquatic animal feed of 99 weight portions, put in mixer and be sufficiently mixed, form the poultry containing sad glyceride lactate feed additive or aquatic animal feed.
The weight sum of the above-described premix material containing sad glyceride lactate feed additive and poultry or aquatic animal feed is 100.
4th kind of mode: concentrate feed addition manner, for 40% poultry or aquatic animal concentrate feed.
A, weigh in 40% poultry or the aquatic animal concentrated feed that the sad glyceride lactate feed additive of 0.75～7.5 weight portion joins 99.25～92.5 weight portions, put in mixer and be sufficiently mixed, form the poultry containing sad glyceride lactate feed additive and aquatic animal concentrated feed.
The weight sum of above-described sad glyceride lactate feed additive and 40% poultry or aquatic animal concentrated feed is 100.
B, by 40% poultry or aquatic animal concentrate feed according to the method in its operation instructions, form the poultry containing sad glyceride lactate feed additive and aquatic animal feed.
Described concentrated feed, is also called protein supplementaling feed, is the mixed feed semi-finished product formulated by protein feeds (fish flour, soybean cake etc.), mineral feed (bone meal stone powder etc.) and additive premix. Mix a certain proportion of energy feed (Semen Maydis, Sorghum vulgare Pers., Fructus Hordei Vulgaris etc.) again and just become the complete feed meeting Animal nutrition needs, there is the advantages such as protein content height (being typically between 30%～50%), nutritional labeling be comprehensive, easy to use. Being typically in ratio shared in perfect compound feed is 20%～40%. Concentrated feed composition: containing following material in its raw material: mineral, including bone meal, stone powder (calcium powder) or conch meal; Trace element, including copper sulfate, manganese sulfate, zinc sulfate, ferrous sulfate, potassium iodide, sodium selenite etc.; Aminoacid, antioxidant, antibiotic, protein feeds and multivitamin etc. It is that poultry life physically well develops soon, meat is good, the required trophic level that is of high nutritive value is calculated according to making, and adopts modern process equipment, is sufficiently mixed by above raw material and makes. This concentrated feed can raise unit as an independent hay varieties supply, and user mixes energy feed by a certain percentage, namely becomes mixed feed after stirring. Such as: the match ratio of layer chicken feed is 30% laying hen concentrated feed, the energy feed of 70%; The match ratio of pig feed is 20% concentrated feed, the energy feed of 80%. Concentrated feed be best suitable for rural households exclusively engaging in a particular productionor trade raise pigs, use of raising chickens. Utilize the grain and side-product that oneself produce, then be equipped with concentrated feed, get final product Direct-fed, decrease unnecessary transit link, abridged edition saving of labor.
It is therein any that described poultry are specially pig, cattle, sheep, chicken, duck, goose.
It is therein any that described aquatic animal is specially the freshwater aquiculture animals such as green grass or young crops, grass, silver carp, flathead, tilapia, Carassius aurutus gibelio, Carassius auratus, shrimp, Eriocheir sinensis.
The outstanding advantages of the present invention is: sad glyceride lactate can effectively suppress the breeding of animal intestinal harmful microbe, promote animal health, reduces and reduces disease incident more than 44%, improves daily gain more than 9.5%, and then improves breeding performonce fo animals. The advantage that this feed additive has safety height, has no drug resistance, it is possible to effectively substitute feeding antibiotic, meets healthy aquaculture developing direction.
Detailed description of the invention
Embodiment 1:
The preparation method of a kind of sad glyceride lactate feed additive, the steps include:
1. weigh that 144g is sad respectively, 92g glycerol, 220g lactic acid, 62g boric acid, 10g hydrogenated resin and 200ml hexamethylene.
2. at N2Under protection, in the reactor of 1L, add boric acid and glycerol.
3.200 DEG C are heated to reflux 3 hours, reduce temperature to room temperature (20-25 DEG C).
4. in reactor, it is sequentially added into sad, hydrogenated resin, hexamethylene again.
5. gradually rise reactor temperature, maintain 150 DEG C, 3 hours.
6. being cooled to room temperature, the 0.1M hydrochloric acid adding 100g stirs 1 hour.
7., after solution-stabilized, open reactor liquid valve door separating and filtering and remove boric acid hydrochloric acid mixed solution.
8. adding in reactor by lactic acid again, at normal pressure 130 DEG C, the response time is 4 hours.
9. open reactor liquor separator valve and isolate aqueous phase.
10.0.5KPa decompression Distillation recovery hexamethylene, isolate hydrogenated resin, namely obtain single sad lactyl-lactic acid glyceride.
Embodiment 2:
The preparation method of a kind of sad glyceride lactate feed additive, the steps include:
1. weigh that 144g is sad respectively, 92g glycerol, 110g lactic acid, 62g boric acid, 10g hydrogenated resin and 200ml hexamethylene.
2., by glycerol, hexamethylene and hydrogenated resin, it is sequentially added into 1L reactor.
3. gradually rising reactor temperature to 150 DEG C, it is sad to be slowly added to.
4., when opening the water that reactor liquor separator valve collection water yield reaches 18ml, in reactor, slowly add lactic acid.
5. at normal pressure 130 DEG C, the response time is 4 hours, collects when water yield reaches 45ml water when reactor liquor separator valve and terminates reaction.
6.0.5KPa decompression Distillation recovery hexamethylene, isolates hydrogenated resin, obtains light yellow clear liquid and namely obtains single sad single glyceride lactate.
Embodiment 3:
The preparation method of a kind of sad glyceride lactate feed additive, the steps include:
1. weigh that 288g is sad respectively, 92g glycerol, 110g lactic acid, 62g boric acid, 10g hydrogenated resin and 200ml hexamethylene.
2. at N2Under protection, in 1L reactor, it is sequentially added into boric acid and glycerol.
3.200 DEG C are heated to reflux 3 hours, reduce temperature to room temperature (20-25 DEG C).
4. in reactor, it is sequentially added into lactic acid, hydrogenated resin and hexamethylene again.
5. gradually rise reactor temperature, maintain 150 DEG C, 3 hours.
6. being cooled to room temperature, the 0.1M hydrochloric acid adding 100g stirs 1 hour.
7., after solution-stabilized, open reactor liquid valve door separating and filtering and remove boric acid hydrochloric acid mixed solution.
8., again by sad addition reactor, at normal pressure 150 DEG C, the response time is 3 hours.
9. open reactor liquor separator valve when isolating 40ml water, stopped reaction.
10.0.5KPa decompression Distillation recovery hexamethylene, isolate hydrogenated resin, obtain the sad single glyceride lactate of yellow liquid that is two.
Embodiment 4:
Single sad lactyl-lactic acid glyceride, single sad single glyceride lactate and two sad single glyceride lactates are scheduled for test according to following table, measure the bacteriostatic activity of sad lactate with Odontothrips loti.
Table 1 sad lactate In Vitro Bacteriostasis effect (mm)
The data of table 1 show, escherichia coli and Salmonella are had inhibitory action by sad lactate, the size of its In Vitro Bacteriostasis circle respectively 13.47～18.29mm and 11.70～15.91mm.
Embodiment 5:
The application in ablactational baby pig feedstuff of a kind of sad glyceride lactate feed additive, the steps include:
The defatted rice bran of the sad lactyl-lactic acid glyceride of list and 50 weight portions that 1, weigh 50 weight portions respectively puts into mix homogeneously in mixer, namely prepares sad glyceride lactate feed additive pre-mixing agent I.
The ablactational baby pig feedstuff of the sad lactate feed additive pre-mixing agent I and 99.9 weight portions that 2, weigh 0.1 weight portion respectively puts in mixer mixs homogeneously, and namely prepares the ablactational baby pig feedstuff containing sad glyceride lactate feed additive I.
The defatted rice bran of the sad single glyceride lactate of list and 50 weight portions that 3, weigh 50 weight portions respectively puts into mix homogeneously in mixer, namely prepares sad glyceride lactate feed additive pre-mixing agent II.
The ablactational baby pig feedstuff of the sad glyceride lactate feed additive pre-mixing agent II and 99.82 weight portions that 4, weigh 0.18 weight portion respectively puts in mixer mixs homogeneously, and namely prepares the ablactational baby pig feedstuff containing sad glyceride lactate feed additive II.
The defatted rice bran of the two sad single glyceride lactates and 50 weight portions that 5, weigh 50 weight portions respectively puts into mix homogeneously in mixer, namely prepares sad glyceride lactate feed additive pre-mixing agent III.
The ablactational baby pig feedstuff of the sad glyceride lactate feed additive pre-mixing agent III and 99.5 weight portions that 6, weigh 0.5 weight portion respectively puts in mixer mixs homogeneously, and namely prepares the ablactational baby pig feedstuff containing sad glyceride lactate feed additive III.
7, select the DLY ablactational baby pig that the body weight of 96 21 disposable wean of age in days is consistent with sex, be randomly divided into 4 groups, be i.e. matched group and test I, II, III group, every component 3 repetition (hurdle), 8, every hurdle.
8, the basal diet trophic level that four groups feed is consistent. Wherein testing I, II, III group to feed respectively containing sad lactate feed additive I, containing sad lactate feed additive II, ablactational baby pig feedstuff containing sad lactate feed additive III, control group fed adds 0.04% kangdisu sulfas pre-mixing agent (kangdisu sulfas content is 10%).
9, feeding ablactational baby pig 21 days, result is table 2 below such as:
Table 2 adds the impact on Growth Performance of Weaning Piglets of the sad glyceride lactate feed additive
Note: colleague shoulder marking-up mother difference person represents significant difference (p < 0.05). Similar below.
It is shown that apply feed additive of the present invention, compared with the matched group adding kangdisu sulfas, it is possible to significantly improve piglet daily gain more than 14.62%; Reduce feed-weight ratio more than 9.48%; Reduce Incidence of Diarrhea 43.66%. Therefore, sad glyceride lactate feed additive can promote piglet growth, improves feed efficiency, reduce disease incident, it is possible to effectively substitute antivirus somatotropic agent. It is therein any that this embodiment is applicable to pig, cattle, sheep, rabbit.
Embodiment 6:
The application in preparing Carassius aurutus gibelio feedstuff of a kind of sad glyceride lactate feed additive, the steps include:
The defatted rice bran of the sad lactyl-lactic acid glyceride of list and 50 weight portions that 1, weigh 50 weight portions respectively puts into mix homogeneously in mixer, namely prepares sad glyceride lactate feed additive pre-mixing agent I.
The Carassius aurutus gibelio feedstuff of the sad lactate feed additive pre-mixing agent I and 99.8 weight portions that 2, weigh 0.2 weight portion respectively puts in mixer mixs homogeneously, and namely prepares the Carassius aurutus gibelio feedstuff containing sad glyceride lactate feed additive I.
The defatted rice bran of the sad single glyceride lactate of list and 50 weight portions that 3, weigh 50 weight portions respectively puts into mix homogeneously in mixer, namely prepares sad glyceride lactate feed additive pre-mixing agent II.
The Carassius aurutus gibelio feedstuff of the sad lactate feed additive pre-mixing agent II and 99.62 weight portions that 4, weigh 0.38 weight portion respectively puts in mixer mixs homogeneously, and namely prepares the Carassius aurutus gibelio feedstuff containing sad glyceride lactate feed additive II.
The defatted rice bran of the two sad single glyceride lactates and 50 weight portions that 5, weigh 50 weight portions respectively puts into mix homogeneously in mixer, namely prepares sad glyceride lactate feed additive pre-mixing agent III.
The Carassius aurutus gibelio feedstuff of the sad lactate feed additive pre-mixing agent III and 99 weight portions that 6, weigh 1 weight portion respectively puts in mixer mixs homogeneously, and namely prepares the Carassius aurutus gibelio feedstuff containing sad glyceride lactate feed additive III.
7, Carassius aurutus gibelio 600 tail selecting body weight to be 9.27 ± 0.06g, is randomly divided into 4 groups, i.e. matched group and test I, II, III group, every component 3 repetition (case), every case 50 tail.
8, the basal diet trophic level that each group feeds is consistent. Testing I, II, III group to feed containing sad glyceride lactate feed additive I, containing sad glyceride lactate feed additive II, Carassius aurutus gibelio feedstuff containing sad glyceride lactate feed additive III, control group fed adds 2mg/kg bambermycin.
9, by the growth test of 8 weeks, sad glyceride lactate feed additive is evaluated as the antivirus somatotropic agent impact on allogynogenetic crucian carp performance. Result is shown in table 3 below.
Table 3 adds the impact on Carassius auratus growth performance of the sad glyceride lactate feed additive
| Project | Blank group | Test I group | Test II group | Test III group |
| Specific growth rate % | 3.66±0.12b | 4.27±0.04a | 4.38±0.09a | 4.32±0.04a |
| Feed coefficient | 1.49±0.05b | 1.31±0.03a | 1.32±0.06a | 1.34±0.02a |
| Survival rate % | 98.10±2.00 | 98.70±2.07 | 99.30±1.09 | 99.50±1.10 |
Result of the test shows, applies feed additive of the present invention, compared with the matched group adding bambermycin, it is possible to improve Carassius auratus specific growth rate 16.67%, feed coefficient reduces by 10.06%. Therefore, sad glyceride lactate feed additive is by improving Carassius auratus serum oxidation resistance, thus promoting growth, improving feed efficiency, it is possible to effectively substitute antivirus somatotropic agent. It is therein any that this embodiment is applicable to the freshwater aquiculture animals such as green grass or young crops, grass, silver carp, flathead, tilapia, Carassius aurutus gibelio, Carassius auratus, shrimp, Eriocheir sinensis.
Claims (5)
1. the preparation method of a sad glyceride lactate feed additive, it is characterised in that: the sad lactyl-lactic acid glyceride of list that sad glyceride lactate is sad by a part and two molecule lactic acid generate with a part glycerine esterification, its molecular formula is C17H30O8, molecular weight: 362.42, the steps include:
A, weigh the glycerol of sad, 92 weight portions of 144 weight portions and the lactic acid of 220 weight portions respectively;
B, at N2Under protection, in reactor, add the boric acid of 62 weight portions and the glycerol of 92 weight portions;
C, 198-202 DEG C is heated to reflux 3-4 hour, reduces temperature room temperature;
D, in reactor, it is sequentially added into the hydrogenated resin of sad, 10 weight portions of 144 weight portions again, the hexamethylene of 200 parts by volume;
E, gradually rise reactor temperature to 148-152 DEG C, maintain 3-4 hour;
F, being cooled to room temperature, the 0.1M hydrochloric acid adding 100 weight portions stirs 58-62 minute;
G, after solution-stabilized, open reactor liquid valve door separating and filtering remove boric acid hydrochloric acid mixed solution;
H, the lactic acid of 220 weight portions being added in reactor, at normal pressure 128-132 DEG C, the response time is 4-5 hour again;
J, open reactor liquor separator valve and isolate aqueous phase;
K, 0.5KPa reduce pressure Distillation recovery hexamethylene, isolate hydrogenated resin, obtain single sad lactyl-lactic acid glyceride feed additive.
2. the preparation method of a sad glyceride lactate feed additive, it is characterised in that: sad glyceride lactate is sad by a part and the sad single glyceride lactate of list of a part lactic acid and the generation of a part glycerine esterification, and its molecular formula is C14H26O6, molecular weight: 290.36, the steps include:
A, weigh the glycerol of sad, 92 weight portions of 144 weight portions and the lactic acid of 110 weight portions respectively;
B, first by the hydrogenated resin of the glycerol of 92 weight portions, the hexamethylene of 200 parts by volume and 10 weight portions, be sequentially added into reactor;
C, gradually rise reactor temperature to 148-152 DEG C, be slowly added to the sad of 144 weight portions;
D, open reactor liquor separator valve and collect water yield when reaching the water of 18 parts by volume, in reactor, slowly add the lactic acid of 110 weight portions;
E, at normal pressure 128-132 DEG C the response time be 4-5 hour, collect when water yield reaches the water of 45 parts by volume when reactor liquor separator valve and terminate reaction;
F, 0.5KPa reduce pressure Distillation recovery hexamethylene, isolate hydrogenated resin, obtain light yellow clear liquid and namely obtain single sad single glyceride lactate.
3. the preparation method of a sad glyceride lactate feed additive, it is characterised in that: sad glyceride lactate is sad by two molecules and two sad single glyceride lactates of a part lactic acid and the generation of a part glycerine esterification, and its molecular formula is C22H40O7, molecular weight: 416.55, the steps include:
A, weigh the glycerol of sad, 92 weight portions of 288 weight portions and the lactic acid of 110 weight portions respectively;
B, at N2Under protection, in reactor, add the boric acid of 62 weight portions and the glycerol of 92 weight portions;
C, 198-202 DEG C is heated to reflux 3 hours, reduces temperature to room temperature;
D, in reactor, it is sequentially added into the hydrogenated resin of the lactic acid of 110 weight portions, 10 weight portions again, the hexamethylene of 200 parts by volume;
E, gradually rise reactor temperature to 150 DEG C, maintain 3-4 hour;
F, being cooled to room temperature, the 0.1M hydrochloric acid adding 100 weight portions stirs 1 hour;
G, after solution-stabilized, open reactor liquid valve door separating and filtering remove boric acid hydrochloric acid mixed solution;
H, again by the sad addition reactor of 288 weight portions, at normal pressure 148-152 DEG C, the response time is 3-4 hour;
J, when opening water that reactor liquor separator valve isolates 40 parts by volume, stopped reaction;
K, 0.5KPa reduce pressure Distillation recovery hexamethylene, isolate hydrogenated resin, obtain the sad glyceride lactate of yellow liquid that is two.
4. the application in animal and fowl fodder of a kind of sad glyceride lactate feed additive described in claims 1 to 3 any one;
Described poultry are that pig, cattle, sheep, chicken, duck, goose are therein any.
5. the application in aquatic animal feed of a kind of sad glyceride lactate feed additive described in claims 1 to 3 any one; Described aquatic animal is that green grass or young crops, grass, silver carp, flathead, tilapia, Carassius aurutus gibelio, Carassius auratus, shrimp, Eriocheir sinensis freshwater aquiculture animal are therein any.
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| WO2023137659A1 (en) * | 2022-01-20 | 2023-07-27 | 南京泛太化工医药研究所 | Series of polymers having properties of acid-sensitive degradation and temperature sensitivity and drug loading composition thereof |
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Effective date of registration: 20181130 Address after: 437199 Xianning High-tech Industrial Park, Xianning City, Hubei Province Patentee after: HUBEI HAOHUA BIOTECHNOLOGY CO., LTD. Address before: 430023 No. 68 Xuefu South Road, Changqing Garden, Hankou, Wuhan, Hubei. Co-patentee before: HUBEI HAOHUA BIOTECHNOLOGY CO., LTD. Patentee before: Wuhan Polytechnic University |