CN104524233A - Biological reaction regulator capable of promoting hematopoietic function and enhancing immunity, preparation and application thereof - Google Patents
Biological reaction regulator capable of promoting hematopoietic function and enhancing immunity, preparation and application thereof Download PDFInfo
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Abstract
The invention discloses a biological reaction regulator capable of promoting hematopoietic function and enhancing immunity, preparation and application thereof. The biological reaction regulator is prepared from the following traditional Chinese medicine raw materials in parts by weight: 10-30 parts of squid ink, 5-30 parts of anoectochilus roxburghii, 5-10 parts of xylariasp, 10-30 parts of dendrobium candidum, 10-30 parts of radix rehmanniae praeparata, 10-30 parts of wolfberries, 10-30 parts of semen cuscutae, 10-30 parts of radix astragali, 10-30 parts of angelica sinensis and 10-30 parts of liquorice. A preparation method of the biological reaction regulator comprises the following steps: mixing the traditional Chinese medicine raw materials, then adding water for immersing for 1.5-3 hours, heating and decocting, then pouring decoction liquid, adding water, heating, decocting and pouring decoction liquid, combining the decoction fluids and filtering to obtain clear liquid, concentrating, spray drying, crushing and screening to obtain the biological reaction regulator capable of promoting hematopoietic function and enhancing immunity. The biological reaction regulator has remarkable effects of protecting leukocyte, promoting differentiation of hematopoietic stem cells and increasing leukocyte.
Description
Technical field
The invention belongs to the field of Chinese medicines, particularly a kind ofly can promote biological response modifier and preparation method thereof that hemopoietic function improves immunity and application, at protection leukocyte, promote hematopoietic stem cell differentiation, there is remarkable result leukocyte increasing aspect.
Background technology
In panoramic immunomodulator, natural immunity regulator causes the great interest of scientist.Polysaccharide (as Cordyceps polysaccharide, astragalus polysaccharides, dendrobium polysaccharide, prepared rehmannia root polysaccharide, polysaccharide-peptide and Radix Angelicae Sinensis polysaccharide etc.) the energy Promote immunity function of natural origin, can as biological regulator.Active polysaccharide is a kind of new and effective immunomodulator, can significantly improve the phagocytic activity of macrophage, strengthens the activity of lymphocyte (T, bone-marrow-derived lymphocyte), plays antiinflammatory, antibacterium, viral infection resisting, Tumor suppression, antidotal effect.
Immunity is a kind of physiological function of body, body relies on this identification of function " oneself " and " non-oneself " composition, thus destroy with repel the antigenic substance (as pathogen, tumor cell) that enters body or body itself produce heterogeneous material (as always extremely and damaged cell).Immunity has protective effect to body substantially, as anti-infectious immunity and tumour immunity.At present, along with the progress of molecular biology and immunopharmacology, Chinese medicine immunologic function, as the important branch of modern medicine, has caused the concern of medical circle and pharmacy circle day by day.
Often adopt bone marrow, the peripheral blood of mobilization or the hematopoietic stem cell transplantation of Cord Blood-Derived to rebuild the hemopoietic system of patient clinically, its process is complicated, risk is high, treatment cost is high.The various diseases prevalence of current blood system is in rising trend, and for Chinese medicine or effective monomer component research, real can be rarely found in conjunction with the research of clinical efficacy and theory of Chinese medical science, do not find promote hemopoietic function, improve immunity biological response modifier in clinical practice.Promote that hemopoietic function improves a biological response modifier for immunity, this Chinese medicine associating prescription has no market any Product Report.
Therefrom medically say, Chinese medicine works in coordination with clinical treatment research from " righting ", " efficacy enhancing and toxicity reducing ", is extended to " anticancer synergia attenuation ", and clearly proposes the Comprehensive Treatment concept of Chinese medicine, embody efficient, low toxicity, the target spot feature widely of some Chinese medicine.The immune system of body is a system of carrying out self regulation by various feedback, and Chinese medicine, to the effect of immunity, mainly transfers overall nonspecific resistance.The immunomodulating of Chinese medicine is multidirectional, and it is mainly " strengthening the body resistance ", by strengthening or regulate the function of body, as promoting that the report that hemopoietic function improves the biological response modifier Chinese medicine composition of immunity does not almost have.
Sepia production can promote bone marrow hematogenesis, and its action pathway can by the immune function of conditioner body, and induction produces cytokine profiles, promotes the propagation of hematopoietic stem cell, CFU CM, and to the differentiation of grain system, promotes the grain system hemopoietic of mice.Ink sepiellae seu sepiae has significant promotion mouse bone marrow cells grain system hemoposieis, and its mechanism of action can by the immune function of conditioner body, and induction body produces grain/Granulocyte microphage colony stimulating factor and cytokine profiles.Shandong Academy of Medical Sciences Liu Zhi etc. also find that Ink sepiellae seu sepiae can protect hematopoietic stem cell, and prevention rat acute radiation sickness, illustrate that Ink sepiellae seu sepiae has radiation resistance, this effect may be relevant with its melanic Characteristics of Free Radicals Produced.
Herba Anoectochili roxburghii clearing away heat and cooling blood, removing dampness and detoxicating, Yin Yang balancing, strengthening the body resistance, the function that yin yang complementarity, skin care of promoting the production of body fluid, QI and blood regulating, the five internal organs, foster longevity prolong life.Enter kidney, liver, lung three warp, body immunity can be improved comprehensively, strengthen human body to the resistance of disease.
Wu Lingcan calms the nerves, hemostasis, blood pressure lowering.Wu Lingcan is containing polysaccharide, and protein, and trace element and the active substance such as ferrum, manganese, stannum, have the synergism of the proliferation and growth promoting hematopoietic stem cell, CFU CM.Embody efficient, nontoxic, the pharmacological action feature widely of Wu Lingcan.
The dendrobium polysaccharide of Herba Dendrobii has the effect of enhancing human body immunity power, and polysaccharide is to the promotion of humoral immunity of organism function, cellular immune function with induce cytokine profiles, is that polysaccharide plays one of mechanism of efficacy effect.The research such as Zhang Zhongjian shows, Herba Dendrobii preparation can significantly increase mouse cell immunologic function and humoral immune function, has good immunoregulation effect.Dendrobium polysaccharide can with rlL-2 synergy, remarkable enhancing Cord blood LAK cell (CBK) and Peripheral Blood from Patients with Malignant LAK cell (PBLAK), in vitro to the killing activity of tumor cell, can be used as biological response modifier and are used for the treatment of tumor.
Radix Angelicae Sinensis polysaccharide all has its proliferation function of promotion to normal or through radiation damage mice pluripotential hemopoietic stem cell (CFU-S).No matter Radix Angelicae Sinensis polysaccharide is administered to donor or receptor Mus, result is consistent.Ge faithful and upright person waits In vitro culture also to prove Radix Angelicae Sinensis polysaccharide energy significant stimulation normal and the grain of bone marrow depression one anemia mice, the propagation of monosystem CFU-GM (CFU-GM), and its mechanism may be relevant with promoting body secretes grain, monosystem colony stimulating factor (GM-CSF).
Polysaccharide in Radix Rehmanniae Preparata resembles for different model of blood dificiency mouse peripheral blood, bone marrow nucleated cell declines all has antagonism, has promote propagation, differentiation to mouse hematopoietic stem cell.About research also demonstrate that prepared rehmannia root polysaccharide can act on the hematopoietic stem cell of different cycles, and participate in the proliferation and differentiation of hematopoietic stem cell from protein and molecular level impact, reach the object of rebuilding and recovering immune function.
After Radix Astragali human peripheral blood transplantation of hematopoietic stem cell, early stage leukocytic reconstruction has facilitation, and its mechanism may be that astragalus polysaccharides has protection and improvement result to marrow stromal cell.
Fructus Lycii and Semen Cuscutae fit applications, have the effect obviously promoting hematopoietic cell proliferation.
At present, in the preventing and treating of stem cell transplantation or cellular immunotherapy complication, the promotion hemopoietic function needing good therapeutic effect badly improves the appearance of immunity Chinese medicine composition.With Chinese herb decoction therapeutic alliance refractory disease, result shows can accelerate patient's hematopoietic reconstitution over the course for the treatment of, reduces complication of transplant.The therapeutic effect that this promotion hemopoietic function improves the biological response modifier Chinese medicine composition of immunity is remarkable.
Summary of the invention
In order to overcome the shortcoming of prior art with not enough, primary and foremost purpose of the present invention is to provide a kind of biological response modifier that hemopoietic function improves immunity that promotes.
Another object of the present invention is to provide above-mentioned promotion hemopoietic function to improve the preparation method of the biological response modifier of immunity.
Another object of the present invention is to provide above-mentioned promotion hemopoietic function to improve the application of the biological response modifier of immunity.
Object of the present invention is achieved through the following technical solutions: a kind of biological response modifier promoting hemopoietic function raising immunity, prepared by the following raw material of Chinese medicine counted by weight: Ink sepiellae seu sepiae 10 ~ 30 parts, Herba Anoectochili roxburghii 5 ~ 30 parts, Wu Ling ginseng 5 ~ 10 parts, Herba Dendrobii 10 ~ 30 parts, Radix Rehmanniae Preparata 10 ~ 30 parts, Fructus Lycii 10 ~ 30 parts, Semen Cuscutae 10 ~ 30 parts, the Radix Astragali 10 ~ 30 parts, Radix Angelicae Sinensis 10 ~ 30 parts, 10 ~ 30 parts, Radix Glycyrrhizae, described helper component is water.
Described promotion hemopoietic function improves the preparation method of the biological response modifier of immunity, comprises the following steps: each component by weight:
(1) Herba Dendrobii 10 ~ 30 parts is got, Radix Rehmanniae Preparata 10 ~ 30 parts, Fructus Lycii 10 ~ 30 parts, Semen Cuscutae 10 ~ 30 parts, the Radix Astragali 10 ~ 30 parts, Radix Angelicae Sinensis 10 ~ 30 parts, 10 ~ 30 parts, Radix Glycyrrhizae, obtains mixture after mixing, add mixture total weight amount 8 ~ 10 times amount water soaking, heating decocts, and inclines and decoction liquor, collects medicinal residues;
(2) medicinal residues that step (1) is collected are added again 8 ~ 9 times of water gaging heating of step (1) mixture total weight amount to decoct, incline and decoction liquor, the decoction liquor obtained with step (1) merges filters, and obtains clear liquid;
(3), after clear liquid step (2) obtained is concentrated, carries out spraying dry, obtain spray drying powder; Spray drying powder being crushed to fineness is 30 ~ 100 orders, obtains effective constituents A;
(4) depletion Anoectochilus roxburghii 5 ~ 30 parts, Wu Ling joins 5 ~ 10 parts of mixing, pulverizes, obtains effective ingredient B;
(5) by Ink sepiellae seu sepiae 10 ~ 30 parts, the effective constituents A that step (3) obtains and the effective ingredient B that step (4) obtains mix, and are promoted the biological response modifier that hemopoietic function improves immunity.
Immersion described in step (1) is preferably immersion 1.5 ~ 3h; Be more preferably 1.5 ~ 2.5h;
Decoction described in step (1) is preferably decoction 1.5 ~ 2h;
Decoction described in step (2) is preferably decoction 1 ~ 2h;
Filtration described in step (2) is preferably filters by medical filtration spin vibration sieve;
It is 1.05 ~ 1.06 that concentrating described in step (3) is preferably concentrated into relative density;
Spray-dired condition described in step (3) is preferably: inlet temperature is 140 ~ 160 DEG C, and leaving air temp is 100 ~ 110 DEG C, nebulizer rotating speed 50 ~ 60rpm, and charging rate is 3 ~ 5mL/min;
It is 30 ~ 100 orders that pulverizing described in step (4) is preferably crushed to fineness.
The biological response modifier that described promotion hemopoietic function improves immunity is promoting that hemopoietic function improves the application in immunity.
The effect of each tcm components raw material that the present invention uses is as follows:
Ink sepiellae seu sepiae: [taste with return through] bitter in the mouth; Property is put down.Return Liver Channel.[function with cure mainly] astringing to arrest bleeding.
The Ink sepiellae seu sepiae melanin contained is made up of 5,6-dihydroxy indole and 5,6-dihydroxy indole-2-carboxylic acid, wherein containing secondary degraded oligosaccharide, a small amount of tryrosinase and a kind of can the melanin enzyme reset of catalysis dopachrome.The people such as Lu Tong study and find that Ink sepiellae seu sepiae has the effect of leukocyte increasing.Pharmacological research: antitumor action; Induction NK, LAK killing activity; Activating macrophage; Induce cytokine profiles; Affect protein kinase activity; Affect red blood cell immunization tumor cell ability; Affect Ca in cell
2+concentration.
Herba Anoectochili roxburghii: [taste with return through] property is flat, sweet in the mouth, nontoxic.Return kidney, liver, lung meridian.[function with cure mainly] clearing away heat and cooling blood, Eradicates wind dampness removing, heart tonifying diuresis, reinforce the kidney, suppressing the hyperactive liver.Cure mainly the diseases such as spitting of blood, bronchitis, nephritis, cystitis, pneumonia, diabetes, hematuria, chyluria, rheumatism, acute infantile convulsion wind, pertussis, venom.Enter kidney, liver, lung three warp, body immunity can be improved comprehensively, strengthen human body to the resistance of disease.
Chemical composition and the content of Herba Anoectochili roxburghii are definite; the material with valuable pharmacological activity is mainly: flavone compound (Quercetin, isorhamnetin), steroid compound, triterpenoid compound (oleanolic acid, ursolic acid); polysaccharide composition; alkaloid, and Cardiac glycosides, esters; taurine etc.; Cardiac glycosides, several amino acids, trace element and inorganic elements etc.Pharmacological research shows that the content of aminoacid and trace element in Herba Anoectochili roxburghii composition is all higher than domestic Radix Panacis Quinquefolii and wild ginseng; They and taurine, polysaccharide composition have the effect of nutrition, defying age, adjustment human body immunity of organism.
Wu Lingcan: [taste with return through] property is flat, sweet in the mouth, nontoxic.GUIXIN, liver, urinary bladder channel.[function with cure mainly] calms the nerves; Hemostasis; Blood pressure lowering.Cure mainly insomnia; Cardiopalmus; Spit blood; Epistaxis; Hypertension; Scald; Tonifying YANG; Flat fat.Wu Ling joins appearance brown or black, diameter 1.5 ~ 8cm, have circular recess with mycelia junction, sclerotium inner white, meat is soft, depart from mycelia gradually after sclerotium grows up to and contact, in umbilicus shape, and containing polysaccharide, protein, and the trace element such as ferrum, manganese, stannum, there is the synergism of the proliferation and growth promoting hematopoietic stem cell, CFU CM.
Herba Dendrobii: [taste with return through] is sweet, is slightly cold.Return stomach, spleen, kidney channel.[function with cure mainly] has reinforcing stomach reg fluid, nourishing YIN and clearing away heat.Herba Dendrobii benefit essence fills out marrow and anticoagulation, vasodilator, " element is to the large opinion of YIN YANG classification of natural phenomena ": " kidney producing bone marrow ".Kidney governing bones, can give birth to marrow, medullization blood.Kidney essense is sufficient, then essence and blood alternate; Deficiency of the kidney, then essence and blood can not demutation.Therefore, kidney and blood have very close relationship.Pharmacological research shows, Herba Dendrobii has effect such as recovery of anticoagulation, vasodilator, promotion hemopoietic function.Sesquiterpene glycoside in Herba Dendrobii can promote the propagation of T cell and B cell in vitro.Dendrobium polysaccharide has immunological enhancement, is a kind of good immunomodulator.Herba Dendrobii comparative study shows, and all can raise cyclophosphamide model mice peripheral white blood cell, strengthens the phagocytic function of macrophage, promotes the transformation of body endolymph, improves non-specific immunity and the cellular immune function of body.
Radix Rehmanniae Preparata: [taste with return through] is sweet, tepor.Return liver, kidney channel.[function with cure mainly] nourishing the blood and yin, vital essence replenishing and marrow benifiting.Prepared rehmannia root polysaccharide resembles for different model of blood dificiency mouse peripheral blood, bone marrow nucleated cell declines all has antagonism, has promote propagation, differentiation to mouse hematopoietic stem cell.
Fructus Lycii: [taste with return through] is sweet, flat.Return liver, kidney channel.[function with cure mainly] nourishing the liver and kidney, replenishing vital essence to improve eyesight.One of the effective ingredient be rich in Fructus Lycii peel lycium barbarum polysaccharide (LBP) has obvious potentiation to hemopoietic function of bone marrow and every Immunological Markers for Cell-Mediated Immunity, the active quantities of superoxide dismutase (SOD) of body blood, liver and muscular tissue can be significantly improved, thus be conducive to the removing of active oxygen, increase the quantity of antibody forming cell, improve antibody effects.Fructus Lycii and Semen Cuscutae fit applications, have the effect obviously promoting hematopoietic cell proliferation.
Semen Cuscutae: [taste with return through] is pungent, sweet is flat.Return liver,kidney,spleen warp.[function with cure mainly] YANG invigorating tonifying YIN, controlling nocturnal emission with astringent drugs reducing urination, improving eyesight, antidiarrheal.Semen Cuscutae ethanol extraction has the effect promoting grain system growth of progenitor cells.Fructus Lycii and Semen Cuscutae fit applications, have the effect obviously promoting hematopoietic cell proliferation.
The Radix Astragali: [taste with return through] is sweet, tepor; Return spleen, lung meridian.[function with cure mainly] benefit defends consolidating superficial resistance, tonifying Qi and lifting yang, promoting pus discharge and tissue regeneration strengthening, inducing diuresis to remove edema.Astragalus polysaccharides can improve the contact between marrow stromal cell and hematopoietic stem cell, and astragalus polysaccharides is dry to mouse hemopoietic, CFU-GM has certain mobilized effects to promote the secretion of the endogenous cytokine such as recombinant human granulocyte colony stimulating factor (G-CSF) to show.After Radix Astragali human peripheral blood transplantation of hematopoietic stem cell, early stage leukocytic reconstruction has facilitation, and its mechanism may be that the Radix Astragali has protection and improvement result to marrow stromal cell.The Radix Astragali can promote bone marrow hematogenesis, can promote the generation of all kinds of hemocyte, growth and maturation process, makes low erythrocyte and number of white blood cells recover normal.The Radix Astragali obviously can strengthen cellular immunization, promotes the lymphocyte transformation that polyhydroxyalkanoate (PHA), concanavalin A, Con A (ConA), phytolacca american agglutinin (PWM) cause.
Radix Angelicae Sinensis: [taste with return through] sweet in the mouth; Pungent; Bitter; Warm in nature.Return liver; The heart; Spleen channel [function with cure mainly] is enriched blood; Invigorate blood circulation; Menstruction regulating and pain relieving; Moisturize cunning.Radix Angelicae Sinensis polysaccharide (APS) promotes the propagation of CFU GM * (CFU-GM) and Multipotential progenitor cell (CFU-S); Promote endotheliocyte GM-CSF, interleukin Ⅲ (IL-3) and interleukin-17 (IL-7) protein expression, show that Radix Angelicae Sinensis polysaccharide (APS) is dry to mouse hemopoietic, CFU-GM has certain mobilized effects.
Radix Glycyrrhizae: [taste with return through] is sweet, flat.Enter spleen, stomach, lung meridian.[function with cure mainly] and middle emergency, lung moistening, detoxifies, coordinating the actions of various ingredients in a prescription.Radix Glycyrrhizae acids main manifestations is the effect strengthening macrophage phagocytic function and strengthen cellular immune function, but has inhibitory action to humoral immune function.The property of medicine of Radix Glycyrrhizae relaxes, and can rise, can fall, can with the various kinds of drug such as tonic, cathartic, cold medicine, warm medicine, the medicine of a cold nature with the use of, and have the effect of the property of medicine of being in harmonious proportion.
The present invention adopts various drug matching to work in coordination with and plays respective pharmacological property; play and promote that hemopoietic function improves biological response modifier effect of immunity; be applied in the treatment of the patient such as HD, hypoimmunity, can produce and promote that hematopoietic stem cell breaks up, protection leukocyte leukocyte increasing improves immunity aspect and has positive role.And this Chinese medicine composition has no side effect, play while improving medicine curative effect and have high safety.
Mechanism of the present invention is: the present invention adopts various drug matching to work in coordination with and plays respective pharmacological property, promote body secretes grain, monosystem colony stimulating factor (GM-CSF), play and promote that hemopoietic function improves biological response modifier effect of immunity, be improved the effect of medicine curative effect.Experimentation shows, Chinese medicine composition In vitro culture of the present invention also proves polysaccharide energy significant stimulation wherein normal and the grain of bone marrow depression anemia mice, the propagation of monosystem CFU-GM (CFU-GM), its mechanism and promotion body secretes grain, monosystem colony stimulating factor (GM-CSF), have and enough promote bone marrow hematogenesis significantly, its action pathway can by the immune function of conditioner body, induction produces cytokine profiles, promote the propagation of hematopoietic stem cell, CFU CM, and to the differentiation of grain system, promote the effect of the grain system hemopoietic of mice.
Compared with the conventional medicine of current clinical treatment, find no the medicine that similar promotion hemopoietic function improves the biological response modifier of immunity temporarily, fill up promotion hemopoietic stem cell proliferation, and the blank of biological response modifier aspect is broken up to grain system, the Supporting Therapy after clinical hematopoietic stem cell transplantation, auxiliary feedback is a good regulating drug.
The present invention, relative to prior art, has following advantage and effect:
(1) experimentation shows, biological response modifier In vitro culture of the present invention also proves the grain of the normal and bone marrow depression anemia mice of polysaccharide energy significant stimulation wherein, the propagation of monosystem CFU-GM (CFU-GM), its mechanism may with promotion body secretes grain, monosystem colony stimulating factor (GM-CSF) is relevant, have and enough promote bone marrow hematogenesis significantly, its action pathway can by the immune function of conditioner body, induction produces cytokine profiles, promote hematopoietic stem cell, the propagation of CFU CM, and break up to grain system, promote the effect of the grain system hemopoietic of mice.
(2) compared with the conventional medicine of current clinical treatment, find no the medicine that similar promotion hemopoietic function improves the biological response modifier of immunity temporarily, fill up the propagation promoting hematopoietic stem cell, CFU CM, and break up to grain system, promote the blank of the drugs with function aspect of grain system hemopoietic, in clinical hematopoietic stem cell transplantation, auxiliary feed back in the preventing and treating of later stage or cellular immunotherapy complication to be a good regulating drug, to have great application prospect.
(3) the promotion hemopoietic function that the present invention obtains improves the application of the biological response modifier of immunity except at protection leukocyte, promotes that hematopoietic stem cell breaks up, leukocyte increasing aspect has remarkable result; Also expanded the application of this biological response modifier in cellular replacement therapy refractory severe autoimmune disease or in the preventing and treating of cellular immunotherapy complication simultaneously.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
Embodiment 1
A kind of preparation promoting the biological response modifier Chinese medicine composition of hemopoietic function raising immunity:
(1) take raw material of Chinese medicine Herba Dendrobii 100g, Radix Rehmanniae Preparata 50g, Fructus Lycii 100g, Semen Cuscutae 50g, Radix Astragali 100g, Radix Angelicae Sinensis 50g, Radix Glycyrrhizae 100g, after mixing, obtain raw material of Chinese medicine mixture; Add each raw material of Chinese medicine mixture total weight amount 8 times amount water soaking 1.5 hours, heating decoction 1.5 hours, inclines and decoction liquor; Medicinal residues add each raw material of Chinese medicine mixture total weight amount 8 times of water gaging heating decoctions 1 hour again, and incline and decoction liquor, the decoction liquor twice decoction obtained merges, and filters, obtain clear liquid by medical filtration spin vibration sieve;
(2), when the clear liquid that step (1) obtains is concentrated into relative density is 1.05, send into spray dryer and carry out spraying dry, it is 140 DEG C that inlet temperature controls, leaving air temp is 100 DEG C, nebulizer rotating speed 50 revs/min, charging rate is 3mL/ minute, obtains spray drying powder 30g; Spray drying powder being crushed to fineness is 100 orders, obtains effective constituents A 1;
(3) depletion Anoectochilus roxburghii 30g, Wu Ling join the dry product mixing of 25g, are crushed to 100 orders, obtain effective ingredient B1;
(4) by Ink sepiellae seu sepiae 100g, the effective constituents A 1 that step (2) obtains and the effective ingredient B1 that step (3) obtains mix, and are promoted the biological response modifier that hemopoietic function improves immunity.
Embodiment 2
A kind of preparation promoting the biological response modifier Chinese medicine composition of hemopoietic function raising immunity:
(1) take raw material of Chinese medicine Herba Dendrobii 50g, Radix Rehmanniae Preparata 100g, Fructus Lycii 50g, Semen Cuscutae 100g, Radix Astragali 50g, Radix Angelicae Sinensis 100g, Radix Glycyrrhizae 50g, after mixing, obtain raw material of Chinese medicine mixture; Add each raw material of Chinese medicine mixture total weight amount 9 times amount water soaking 2.0 hours, heating decoction 2.0 hours, inclines and decoction liquor; Medicinal residues add each raw material of Chinese medicine mixture total weight amount 9 times of water gaging heating decoctions 2.0 hours again, and incline and decoction liquor, the decoction liquor twice decoction obtained merges, and filters, obtain clear liquid by medical filtration spin vibration sieve;
(2), when the clear liquid that step (1) obtains is concentrated into relative density is 1.05, send into spray dryer and carry out spraying dry, it is 140 DEG C that inlet temperature controls, leaving air temp is 100 DEG C, nebulizer rotating speed 50 revs/min, charging rate is 3mL/ minute, obtains spray drying powder 50g; Spray drying powder being crushed to fineness is 80 orders, obtains effective constituents A 2;
(3) depletion Anoectochilus roxburghii 120g, Wu Ling join the dry product mixing of 40g, and being crushed to fineness is 80 orders, obtains effective ingredient B2;
(4) by Ink sepiellae seu sepiae 50g, the effective constituents A 2 that step (2) obtains and the effective ingredient B2 that step (3) obtains mix, and obtain a kind of biological response modifier that hemopoietic function improves immunity that promotes.
Embodiment 3
A kind of preparation promoting the biological response modifier Chinese medicine composition of hemopoietic function raising immunity:
(1) take raw material of Chinese medicine Herba Dendrobii 120g, Radix Rehmanniae Preparata 100g, Fructus Lycii 100g, Semen Cuscutae 120g, Radix Astragali 120g, Radix Angelicae Sinensis 100g, Radix Glycyrrhizae 50g, after mixing, obtain raw material of Chinese medicine mixture; Add each raw material of Chinese medicine mixture total weight amount 10 times amount water soaking 2.5 hours, heating decoction 2.0 hours, inclines and decoction liquor; Medicinal residues add each raw material of Chinese medicine mixture total weight amount 9 times of water gagings again and heat 1.5 hours, incline and decoction liquor, and the decoction liquor twice decoction obtained merges, and filter, obtain clear liquid by medical filtration spin vibration sieve;
(2) when the clear liquid that step (1) obtains is concentrated into relative density is 1.06, send into spray dryer, carry out spraying dry, it is 160 DEG C that inlet temperature controls, leaving air temp is 110 DEG C, nebulizer rotating speed 60 revs/min, charging rate is 5mL/ minute, obtains spray drying powder 80g; Spray drying powder being crushed to fineness is 50 orders, obtains effective constituents A 3;
(3) depletion Anoectochilus roxburghii 40g, Wu Ling join the dry product mixing of 30g, and being crushed to fineness is 50 orders, obtains effective ingredient B3;
(4) by Ink sepiellae seu sepiae 40g, the effective constituents A 3 that step (2) obtains and the effective ingredient B3 that step (3) obtains mix, and are promoted the biological response modifier that hemopoietic function improves immunity.
Embodiment 4
A kind of preparation promoting the biological response modifier Chinese medicine composition of hemopoietic function raising immunity:
(1) take raw material of Chinese medicine Herba Dendrobii 100g, Radix Rehmanniae Preparata 100g, Fructus Lycii 100g, Semen Cuscutae 100g, Radix Astragali 100g, Radix Angelicae Sinensis 100g, Radix Glycyrrhizae 100g, after mixing, obtain raw material of Chinese medicine mixture; Add each raw material of Chinese medicine mixture total weight amount 9.5 times amount water soaking 2.0 hours, heating decoction 2.0 hours, inclines and decoction liquor; Medicinal residues add each raw material of Chinese medicine mixture total weight amount 9 times of water gagings again and heat 2.0 hours, incline and decoction liquor, and the decoction liquor twice decoction obtained merges, and filter, obtain clear liquid by medical filtration spin vibration sieve;
(2) when the clear liquid that step (1) obtains is concentrated into relative density is 1.05, send into spray dryer, carry out spraying dry, it is 160 DEG C that inlet temperature controls, leaving air temp is 110 DEG C, nebulizer rotating speed 60 revs/min, charging rate is 5mL/ minute, obtains spray drying powder 100g; Spray drying powder being crushed to fineness is 30 orders, obtains effective constituents A 4;
(3) depletion Anoectochilus roxburghii 80g, Wu Ling join the dry product mixing of 20g, and being crushed to fineness is 30 orders, obtains effective ingredient B4;
(4) by Ink sepiellae seu sepiae 40g, the effective constituents A 4 that step (2) obtains and the effective ingredient B4 that step (3) obtains mix, and are promoted the biological response modifier that hemopoietic function improves immunity.
Comparative example 1
(1) take raw material of Chinese medicine Herba Dendrobii 100g, Radix Rehmanniae Preparata 50g, Fructus Lycii 100g, Semen Cuscutae 50g, Radix Astragali 100g, Radix Angelicae Sinensis 50g, Radix Glycyrrhizae 100g, after mixing, obtain raw material of Chinese medicine mixture; Add each raw material of Chinese medicine mixture total weight amount 8 times amount water soaking 1.5 hours, heating decoction 1.5 hours, inclines and decoction liquor; Medicinal residues add each raw material of Chinese medicine mixture total weight amount 8 times of water gagings again and heat 1 hour, incline and decoction liquor, and the decoction liquor twice decoction obtained merges, and filter, obtain clear liquid by medical filtration spin vibration sieve;
(2), when the clear liquid that step (1) obtains is concentrated into relative density is 1.05, enter spray dryer, inlet temperature controls 140 DEG C, leaving air temp 100 DEG C, nebulizer rotating speed 50 revs/min, charging rate is 3mL/ minute, obtains spray drying powder 100g; Spray drying powder being crushed to fineness is 100 orders, obtains the Chinese medicine composition of biological response modifier.
Effect example 1
Treatment disease effect analysis: a kind of biological response modifier promoting hemopoietic function raising immunity, at protection leukocyte, promotes the effect of hematopoietic stem cell differentiation, leukocyte increasing aspect.
Leukocyte increasing experimental design of the present invention carries out the research of comparative grouping/treatment to the leukopenia that 69 routine adult's a variety of causes cause, and is divided into 3 groups respectively.The embodiment 1 be respectively containing Ink sepiellae seu sepiae, Herba Anoectochili roxburghii, Wu Ling ginseng composition is routine as " biological response modifier group " 23; The comparative example 1 of composition is joined as A group 23 example without Ink sepiellae seu sepiae, Herba Anoectochili roxburghii, Wu Ling; General leukocyte increasing treatment group be B group 23 example as a control group, use batilol 50mg, one month three times are oral, leucogen 20mg, and three times on the one are oral, treat 6 weeks.
" biological response modifier group ", every day 3 doses is oral, treats 6 weeks, " biological response modifier group " should compare with A group, observe hemogram recovery situation after 6 weeks.Curative effect determinate standard: in 4 weeks, total white blood cells rises to 4 × 10
9individual/more than L is effective, and in 6 weeks, total white blood cells rises to 4 × 10
9individual/more than L is that effectively 6 weeks lower than 4 × 10
9individual/L is invalid.
" biological response modifier group ", every day 2 doses is oral, treats 6 weeks, " biological response modifier group " should compare with B matched group, observe hemogram recovery situation after 6 weeks.Curative effect determinate standard: in 4 weeks, total white blood cells rises to 4 × 10
9individual/more than L is effective, and in 6 weeks, total white blood cells rises to 4 × 10
9individual/more than L is that effectively 6 weeks lower than 4 × 10
9individual/L is invalid.Wherein, adult's total white blood cells is in (4.0 ~ 10.0) × 10
9individual/L.
Detailed results is in table 1.
The result that table 13 treatment group therapeutic effect paired observations are analyzed
As seen from the data in Table 1; total effective rate 95.65% containing the present invention's " a kind of biological response modifier promoting hemopoietic function raising immunity " treatment group; comparatively A group, the contrast of B group can make hemogram recovery situation comparatively remarkable, and at protection leukocyte, leukocyte increasing aspect has remarkable result.
Flow cytometer ISHAGE method is adopted to detect CD34+ cell percentage for predicting hematopoietic stem cell differentiation, multiplication capacity.The CD34+ cell percentages average that ISHAGE method detects treatment " biological response modifier " group, A group and B group after 6 weeks is respectively (0.28 ± 0.070) %, (0.01 ± 0.006) % and (0.01 ± 0.001) % (P < 0.01), and the application that the promotion hemopoietic function that the present invention obtains improves the biological response modifier of immunity has remarkable result in the differentiation of promotion hematopoietic stem cell, multiplication capacity.
Above data result shows, biological response modifier of the present invention has the differentiation of promotion hematopoietic stem cell, protection leukocyte leukocyte increasing improves immunity aspect and has positive role.
The present invention adopts this Chinese medicine composition to have no side effect and optimization blends, the pharmacological property that collaborative performance is respective, is applied in the treatment of the patient such as HD, hypoimmunity, can produce and promote that hemopoietic function improves the effect of immunity." a kind of promote that hemopoietic function improves the biological response modifier of immunity " propagation promoting hematopoietic stem cell, CFU CM should be filled up, and to the differentiation of grain system, promote the blank of the biological response modifier aspect of grain system hemoposieis.Simultaneously for patient clinical hematopoietic stem cell transplantation, auxiliary feed back after or the Supporting Therapy of cellular immunotherapy be also a good regulating drug, have great application prospect.
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; the pharmaceutical dosage form, preparation method etc. done under other any does not deviate from spirit of the present invention and principle are done to change, modify, substitute, combine, are simplified; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (9)
1. one kind promotes that hemopoietic function improves the biological response modifier of immunity, it is characterized in that being prepared by the following raw material of Chinese medicine counted by weight: Ink sepiellae seu sepiae 10 ~ 30 parts, Herba Anoectochili roxburghii 5 ~ 30 parts, Wu Ling ginseng 5 ~ 10 parts, Herba Dendrobii 10 ~ 30 parts, Radix Rehmanniae Preparata 10 ~ 30 parts, Fructus Lycii 10 ~ 30 parts, Semen Cuscutae 10 ~ 30 parts, the Radix Astragali 10 ~ 30 parts, Radix Angelicae Sinensis 10 ~ 30 parts, 10 ~ 30 parts, Radix Glycyrrhizae.
2. promotion hemopoietic function according to claim 1 improves the preparation method of the biological response modifier of immunity, it is characterized in that comprising the following steps: each component by weight:
(1) Herba Dendrobii 10 ~ 30 parts is got, Radix Rehmanniae Preparata 10 ~ 30 parts, Fructus Lycii 10 ~ 30 parts, Semen Cuscutae 10 ~ 30 parts, the Radix Astragali 10 ~ 30 parts, Radix Angelicae Sinensis 10 ~ 30 parts, 10 ~ 30 parts, Radix Glycyrrhizae, obtains mixture after mixing, add mixture total weight amount 8 ~ 10 times amount water soaking, heating decocts, and inclines and decoction liquor, collects medicinal residues;
(2) medicinal residues that step (1) is collected are added again 8 ~ 9 times of water gaging heating of step (1) mixture total weight amount to decoct, incline and decoction liquor, the decoction liquor obtained with step (1) merges filters, and obtains clear liquid;
(3), after clear liquid step (2) obtained is concentrated, carries out spraying dry, obtain spray drying powder; Spray drying powder being crushed to fineness is 30 ~ 100 orders, obtains effective constituents A;
(4) depletion Anoectochilus roxburghii 5 ~ 30 parts, Wu Ling joins 5 ~ 10 parts of mixing, pulverizes, obtains effective ingredient B;
(5) by Ink sepiellae seu sepiae 10 ~ 30 parts, the effective constituents A that step (3) obtains and the effective ingredient B that step (4) obtains mix, and are promoted the biological response modifier that hemopoietic function improves immunity.
3. preparation method according to claim 2, is characterized in that: the immersion described in step (1) is immersion 1.5 ~ 3h.
4. preparation method according to claim 2, is characterized in that: the decoction described in step (1) is decoction 1.5 ~ 2h.
5. preparation method according to claim 2, is characterized in that: the decoction described in step (2) is decoction 1 ~ 2h.
6. preparation method according to claim 2, is characterized in that: it is 1.05 ~ 1.06 that the simmer down to described in step (3) is concentrated into relative density.
7. preparation method according to claim 2, it is characterized in that: the spray-dired condition described in step (3) is: inlet temperature is 140 ~ 160 DEG C, leaving air temp is 100 ~ 110 DEG C, nebulizer rotating speed 50 ~ 60rpm, and charging rate is 3 ~ 5mL/min.
8. preparation method according to claim 2, is characterized in that: the pulverizing described in step (4) is 30 ~ 100 orders for being crushed to fineness.
9. promotion hemopoietic function according to claim 1 improves the application of biological response modifier in promotion hemopoietic function raising immunity of immunity.
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