CN104523956A - Mixed preparation of bacillus pumilus and immunopotentiator - Google Patents
Mixed preparation of bacillus pumilus and immunopotentiator Download PDFInfo
- Publication number
- CN104523956A CN104523956A CN201410751286.7A CN201410751286A CN104523956A CN 104523956 A CN104523956 A CN 104523956A CN 201410751286 A CN201410751286 A CN 201410751286A CN 104523956 A CN104523956 A CN 104523956A
- Authority
- CN
- China
- Prior art keywords
- bacillus pumilus
- parts
- mix preparation
- radix
- immunostimulant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention relates to a mixed preparation of bacillus pumilus and an immunopotentiator. The mixed preparation consists of effective components, that is, bacillus pumilus and the immunopotentiator, wherein the bacillus pumilus has a quorum sensing system inhibition function and has the preservation number of CCTCC M 2013240; the immunopotentiator is vitamin C, astragalus membranaceus, eucommia ulmoides, codonopsis pilosula, angelica sinensis, honeysuckle, radix bupleuri, smoked plum, pomegranate bark and schisandra chinensis. The mixed preparation has the advantages that the ability of fish bodies in resisting aeromonas hydrophila infection is remarkably improved, the organism immunity of cultured fishes is improved, the growth of pathogenic entero becteria of fishes is remarkably inhibited, the rapid fish body growth is promoted, and the weight gain rate and the disease resistance of cultured fishes are increased.
Description
Technical field
The present invention relates to microorganism field, specifically, is a kind of Bacillus pumilus and immunostimulant mix preparation.
Background technology
Bacterial disease is one of major reason of puzzlement culture fishery development always.In recent years, the intensive production model of high density is while promotion aquatic products industry improves output, and the chance also making bacterial disease occur increases, frequency increases, harm increasing; In addition, antibiotic usage causes drug resistance, drug residue and the destruction to ecological environment also become the focus of social concerns.Therefore, find antibiotic alternative medicine, explore new controlling mode and become Aquatic product disease control research one of important directions.
Quorum sensing (Quorum sensing is called for short QS) refers to the antibacterial spontaneous generation of energy and discharges some specific signaling molecules, the change of perception self concentration, and then the expression of regulation and control related gene.At present, regulating the signaling molecule of bacterial community induction to mainly contain three kinds, (a) N-acyl homoserine lactones (AHLs), is the signaling molecule of the quorum sensing be extensively present in gram negative bacteria; (b) autologous induction peptide (Autoinducing peptides, AIP), AIP is a class short peptide molecules, is signaling molecule common in gram-positive bacterium; C () auto-inducer class Ⅱmolecule (Auto inducing II, AI2) is representative by the signaling molecule of luxS enzymatic AI 2 system with Fermi operator (Vibrio fischeri).Research shows, in gram negative bacteria, as Pseudomonas aeruginosa (Pseudomonas aeruginosa), Aeromonas hydrophila (Aeromonas hydrophila) etc., the behaviors such as the formation of its biofilm, harmful toxins generation, virulence factor release are all subject to the adjustment of AHLs class signaling molecule.The people such as Dong (2000) have found aiiA gene in bacillus cereus (Bacillus cereus), can synthesize AHLs signaling molecule digestive enzyme, block the transmission of QS signaling molecule between antibacterial; Yin Shouliang etc. (2009) filter out a strain from Jiaozhou Bay and can to degrade the fungus of AHLs signaling molecule, show that Marine microorganism also can as the potential object of study of QS inhibitor; Bhavanath etc. (2013) find that the crude extract of Ramulus et folium taxi cuspidatae shape Asparagus toxiformis (Delile) Coll et Harv (Asparagopsis taxiformis) can be degraded AHLs signaling molecule, and have certain inhibitory action to aeruginosa biofilm.Defoirdt etc. (2011) have isolated the bacillus cereus that vibrio harveyi (Vibrio harveyi) QS signaling molecule can be suppressed to transmit first from the intestinal of the aquatic animal such as Penaeus vannamei and marine seabass, Natrah etc. (2011) isolate the microalgae that a strain can suppress AHLs signaling molecule, this microalgae can block vibrio harveyi QS system, reduces its pathogenicity.These researchs are inquire into the support providing theoretical foundation and science data from quorum sensing angle prevention and control Aquatic product bacterial disease.Bacillus cereus (Bcaillus sp) has the features such as easy production, just transport, storage endurance, aquaculture can play purify water, the effect such as disease preventing and treating and growth promoting effects, be conventional strains of probiotic microorganism.
Chinese patent literature CN103525723A discloses one and has the inhibiting Bacillus pumilus of intervention school-based, has the effect of anti-bacteria quorum sensing, can be used for the microorganism formulation preparing control Aquatic product bacterial disease.But there is significantly control Aquatic product bacterial disease, the Bacillus pumilus of improving survival rate and immunostimulant mix preparation yet there are no report about a kind of.
Summary of the invention
The object of the invention is for deficiency of the prior art, a kind of Bacillus pumilus and immunostimulant mix preparation are provided.
For achieving the above object, the technical scheme that the present invention takes is: a kind of Bacillus pumilus and immunostimulant mix preparation, the effective ingredient of described mix preparation is made up of Bacillus pumilus and immunostimulant, described Bacillus pumilus is for having the inhibiting Bacillus pumilus of intervention school-based, deposit number is CCTCC M 2013240, described immunostimulant is vitamin C, the Radix Astragali, the Cortex Eucommiae, Radix Codonopsis, Radix Angelicae Sinensis, Flos Lonicerae, Radix Bupleuri, Fructus Mume, Pericarpium Granati and Fructus Schisandrae Chinensis, in described mix preparation, the weight portion of Bacillus pumilus and immunostimulant is respectively: Bacillus pumilus 14-16 part, vitamin C 10-12 part, Radix Astragali 7-8 part, Cortex Eucommiae 4-5 part, Radix Codonopsis 2-4 part, Radix Angelicae Sinensis 3-5 part, Flos Lonicerae 6-8 part, Radix Bupleuri 9-10 part, Fructus Mume 3-5 part, Pericarpium Granati 4-6 part, Fructus Schisandrae Chinensis 9-10 part.
Preferably, in described mix preparation, the weight portion of Bacillus pumilus and immunostimulant is respectively: Bacillus pumilus 16 parts, vitamin C 12 parts, the Radix Astragali 8 parts, the Cortex Eucommiae 5 parts, Radix Codonopsis 4 parts, Radix Angelicae Sinensis 5 parts, Flos Lonicerae 8 parts, Radix Bupleuri 10 parts, Fructus Mume 5 parts, Pericarpium Granati 6 parts, Fructus Schisandrae Chinensis 10 parts.
Preferably, the preparation method of described mix preparation is that described Bacillus pumilus is made microbial bacterial agent through liquid fermentation or solid fermentation, and in described microbial bacterial agent, Bacillus pumilus viable count is 10
9-10
11cfu/g, after described microbial bacterial agent, vitamin C, the Radix Astragali, the Cortex Eucommiae, Radix Codonopsis, Radix Angelicae Sinensis, Flos Lonicerae, Radix Bupleuri, Fructus Mume, Pericarpium Granati and Fructus Schisandrae Chinensis were pulverized 100 mesh sieves respectively, according to weight mixing, obtains described mix preparation.
Preferably, described mix preparation uses as feeding additive aquatic animal or aquaculture water body additives.
Preferably, described Aquatic product is freshwater fish.
Preferably, the addition of described mix preparation in feedstuff is 1%-3%.
The invention has the advantages that:
Mix preparation of the present invention to the immune protective rate of Fish up to 73-90%; the infection ability of fish body opposing Aeromonas hydrophila can be significantly improved; strengthen the immunity of organisms of cultured fishes; the growth of remarkable suppression fish intestines pathogenic bacterium; promote that fish body grows fast, improve rate of body weight gain and the premunition of cultured fishes.
Detailed description of the invention
Below in conjunction with embodiment, detailed description of the invention provided by the invention is elaborated.
The invention provides a kind of Bacillus pumilus and immunostimulant mix preparation, the effective ingredient of described mix preparation is made up of Bacillus pumilus and immunostimulant, described Bacillus pumilus is for having the inhibiting Bacillus pumilus of intervention school-based, deposit number is CCTCC M 2013240, has confirmed that it has intervention school-based inhibitory action.Described immunostimulant is vitamin C, the Radix Astragali, the Cortex Eucommiae, Radix Codonopsis, Radix Angelicae Sinensis, Flos Lonicerae, Radix Bupleuri, Fructus Mume, Pericarpium Granati and Fructus Schisandrae Chinensis, and in described mix preparation, the weight portion of Bacillus pumilus and immunostimulant is respectively: Bacillus pumilus 14-16 part, vitamin C 10-12 part, Radix Astragali 7-8 part, Cortex Eucommiae 4-5 part, Radix Codonopsis 2-4 part, Radix Angelicae Sinensis 3-5 part, Flos Lonicerae 6-8 part, Radix Bupleuri 9-10 part, Fructus Mume 3-5 part, Pericarpium Granati 4-6 part, Fructus Schisandrae Chinensis 9-10 part.
Described Bacillus pumilus (Bacillus pumilus), depositary institution: China typical culture collection center (CCTCC), address: Wuhan University's school of life and health sciences preservation center, preservation date on June 2nd, 2013, deposit number is CCTCC NO:M 2013240.Confirm that it has intervention school-based inhibitory action.
The preparation method of described mix preparation is that described Bacillus pumilus is made microbial bacterial agent through liquid fermentation or solid fermentation, and in described microbial bacterial agent, Bacillus pumilus viable count is 10
9-10
11cfu/g, after described microbial bacterial agent, vitamin C, the Radix Astragali, the Cortex Eucommiae, Radix Codonopsis, Radix Angelicae Sinensis, Flos Lonicerae, Radix Bupleuri, Fructus Mume, Pericarpium Granati and Fructus Schisandrae Chinensis were pulverized 100 mesh sieves respectively, according to weight mixing, obtains described mix preparation.
Embodiment 1 Bacillus pumilus liquid fermentation
Short and small bud pole bacteria strain is pressed the order cultivation of inclined-plane, liquid seeds, liquid fermentation, slant medium is conventional nutrient agar culture medium, and liquid seed culture medium is conventional nutrient meat soup fluid medium; Liquid fermentation medium formula (W/V): glucose 2%, soybean cake powder 8%, Semen Maydis pulp 1%, MgSO
47H
2o0.02%, NaCl 0.4%, KH
2pO
40.1%, MnCl
20.04%, fermentation temperature 30 DEG C, aerlbic culture 24-48h.Obtain the zymocyte liquid of high concentration, wherein viable count 10
9-10
11cfu/g.
Also by zymocyte liquid centrifugal segregation fermentation liquid, can obtain wet bacterium mud, after adding hat resistant pretectants, spraying dry, obtains solid mycopowder, viable count 10
9-10
11cfu/g.
Embodiment 2 Bacillus pumilus solid fermentation
Short and small bud pole bacteria strain is pressed the order cultivation of inclined-plane, liquid seeds, solid fermentation, slant medium is conventional nutrient agar culture medium, and liquid seed culture medium is conventional nutrient meat soup fluid medium.Be inoculated in solid-state fermentation culture medium with the inoculum concentration of 5-10% by seed culture fluid and carry out solid fermentation, fermentation time is 24-48h, fermentation temperature 30 DEG C, and tunning obtains Bacillus pumilus microbial bacterial agent after drying and pulverizing.Wherein viable count 10
9-10
11cfu/g.
Wherein solid-state fermentation culture medium is by expecting to form with water, and described material, by gross mass mark 100%, comprises Testa oryzae 10%, wheat bran 25%, bean cake 32%, Semen Maydis powder 30%, yeast extract 2%, NaCl 1%, and material-water ratio is 1:1.
The preparation of embodiment 3 mix preparation
Described Bacillus pumilus is made microbial bacterial agent through solid fermentation, and in described microbial bacterial agent, Bacillus pumilus viable count is 10
9-10
11cfu/g, by described Bacillus pumilus microbial inoculum 16 parts, vitamin C 12 parts, the Radix Astragali 8 parts, the Cortex Eucommiae 5 parts, Radix Codonopsis 4 parts, Radix Angelicae Sinensis 5 parts, Flos Lonicerae 8 parts, Radix Bupleuri 10 parts, Fructus Mume 5 parts, Pericarpium Granati 6 parts, Fructus Schisandrae Chinensis 10 parts.After pulverizing 100 mesh sieves respectively, according to weight mixing, obtain described mix preparation.
The preparation of embodiment 4 mix preparation
Described Bacillus pumilus is made microbial bacterial agent through liquid fermentation, and in described microbial bacterial agent, Bacillus pumilus viable count is 10
9-10
11cfu/g, by described Bacillus pumilus microbial inoculum 14 parts, vitamin C 10 parts, the Radix Astragali 7 parts, the Cortex Eucommiae 4 parts, Radix Codonopsis 2 parts, Radix Angelicae Sinensis 3 parts, Flos Lonicerae 6 parts, Radix Bupleuri 9 parts, Fructus Mume 3 parts, Pericarpium Granati 4 parts, Fructus Schisandrae Chinensis 9 parts.After pulverizing 100 mesh sieves respectively, according to weight mixing, obtain described mix preparation.
The preparation of embodiment 5 mix preparation
Described Bacillus pumilus is made microbial bacterial agent through solid fermentation, and in described microbial bacterial agent, Bacillus pumilus viable count is 10
9-10
11cfu/g, by described Bacillus pumilus microbial inoculum 15 parts, vitamin C 11 parts, the Radix Astragali 7 parts, the Cortex Eucommiae 4 parts, Radix Codonopsis 3 parts, Radix Angelicae Sinensis 4 parts, Flos Lonicerae 7 parts, Radix Bupleuri 10 parts, Fructus Mume 4 parts, Pericarpium Granati 5 parts, Fructus Schisandrae Chinensis 10 parts.After pulverizing 100 mesh sieves respectively, according to weight mixing, obtain described mix preparation.
The water splashing effect experimental of embodiment 6 mix preparation
Carassius auratus, buys from freshwater fish culturing center, Xuzhou, body weight 100 ± 0.5g, feeds in advance 10 days, without any Aeromonas hydrophila symptom.Feedstuff is bought from Ningbo Tianbang Co., Ltd, feeds according to 3% of body weight.
First raise and train before the test and within one week, determine to close Aeromonas hydrophila symptom without appointing, then get the Carassius auratus 90 of body weight (100 ± 2.3) g, be divided into three groups often to organize three repetitions at random, often organize 10, cultivation, at 700 × 500 × 50mm, in volume 90L aquarium, keeps water temperature 16 ~ 20 DEG C.
Experimental design three groups, totally 90, often organize 10, three repetition, matched group: in water body, adding the Aeromonas hydrophila bacterium liquid 100mL of cultivation, (finally bacterial concentration reaches 10 after dilution
8cfu/mL, formerly experiment determines that the absolute lethal concentration of Aeromonas hydrophila is 1 × 10
8cfu/mL), test group adds the mix preparation 10g of the present invention that Aeromonas hydrophila bacterium liquid 100mL and embodiment 3 prepare, and blank group does not add anything in blank water body.Change water every three days, add the tap water of the gas that exposed to the sun, and the bacterium liquid again cultivated, the upgrowth situation of record Carassius auratus and death toll.
In the breeding cycle of two weeks, blank group without any the phenomena of mortality; The all samples of matched group are all dead in 9 days, and add the test group of Aeromonas hydrophila and mix preparation of the present invention in water body, and after seven days, reach stable, final survival rate is 90%.
Embodiment 7 mix preparation growth promotion and challenge viral dosage
Growth experiment: the Carassius auratus that test and Selection health, specification, weight are basically identical, individual just counterpoise is about 50g.Random packet, be provided with matched group, test one group, test two groups, test three groups and test four groups, often group establishes 3 parallel tests, every parallel group 50 tail test fish, totally 6 net cages (specification is 250cm × 150cm × 100cm).After Carassius auratus domestication 30d, matched group is thrown something and fed normal diet, test the feedstuff of one group of interpolation 3.0% percentage by weight mix preparation of the present invention (embodiment 3) of throwing something and feeding, test the feedstuff of two groups of interpolation 1.0% percentage by weight mix preparations of the present invention of throwing something and feeding, test the feedstuff of three groups of interpolation 3.0% percentage by weight Bacillus pumilus of the present invention (not comprising the mix preparation of immunostimulant) of throwing something and feeding, test the feedstuff of four groups of interpolation 3.0% percentage by weight immunostimulants (not comprising the immunostimulant of Bacillus pumilus) of throwing something and feeding, raise 56d, measure the impact of its growth.
Challenge viral dosage: matched group, test one group, test two groups, test three groups and test four groups often group randomly draw 15 tail Carassius auratuss.1 × 10
8cfu/mL Aeromonas hydrophila, by every 100g fish heavy lumbar injection bacterium liquid 1.0ml, after put in the cement pit net cage of same specification volume (specification is 100cm × 100cm × 100cm) in a suitable place to breed, and ensure sufficient oxygen, observe fish body death condition every day, observe 10d, statistics survival rate.
Concrete result of the test sees the following form 1.
The contrast of table 1 three groups of rates of body weight gain and mortality rate
| Matched group | Test one group | Test two groups | Test three groups | Test four groups | |
| Rate of body weight gain | 5.02％ | 15.48％ | 10.25％ | 8.34％ | 9.15％ |
| Survival rate | 0 | 86％ | 73％ | 61％ | 53％ |
Result of the test shows, in feedstuff, add the mix preparation of the present invention of 3.0%, 1.0% test one group and test two groups of rates of body weight gain reach 15.48% and 10.25%, compared with matched group, and P < 0.05.After lumbar injection Aeromonas hydrophila 10d, matched group Carassius auratus is all dead, and the test one group of mix preparation of the present invention of feeding and test two groups of final survival rates are respectively 86% and 73%, compared with matched group, and P < 0.01.Simultaneously, the immune indexes such as superoxide dismutase activity, lysozyme content, antibacterial activity tested in one group and test two groups of Carassius auratus serum is all significantly higher than matched group (P < 0.05), and the quantity of the pathogenic bacterium such as the total number of bacteria in intestinal, escherichia coli, Aeromonas hydrophila is significantly lower than matched group (P < 0.05).Rate of body weight gain and the survival rate of testing one group are also significantly higher than the test three groups and test four groups that are used alone Bacillus pumilus or immunostimulant, show mix preparation of the present invention to use simultaneously there is the inhibiting Bacillus pumilus of intervention school-based and immunostimulant, fish body anti-infection ability can be significantly improved, strengthen the immunity of organisms of cultured fishes, promote that fish body grows fast simultaneously.
Above experimental result display; mix preparation of the present invention to the immune protective rate of Fish up to 73-90%; the infection ability of fish body opposing Aeromonas hydrophila can be significantly improved; strengthen the immunity of organisms of cultured fishes; the growth of remarkable suppression fish intestines pathogenic bacterium; promote that fish body grows fast, improve rate of body weight gain and the premunition of cultured fishes.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the prerequisite not departing from the inventive method; can also make some improvement and supplement, these improve and supplement and also should be considered as protection scope of the present invention.
Claims (6)
1. a Bacillus pumilus and immunostimulant mix preparation, it is characterized in that, the effective ingredient of described mix preparation is made up of Bacillus pumilus and immunostimulant, described Bacillus pumilus is for having the inhibiting Bacillus pumilus of intervention school-based, deposit number is CCTCC M 2013240, described immunostimulant is vitamin C, the Radix Astragali, the Cortex Eucommiae, Radix Codonopsis, Radix Angelicae Sinensis, Flos Lonicerae, Radix Bupleuri, Fructus Mume, Pericarpium Granati and Fructus Schisandrae Chinensis, in described mix preparation, the weight portion of Bacillus pumilus and immunostimulant is respectively: Bacillus pumilus 14-16 part, vitamin C 10-12 part, Radix Astragali 7-8 part, Cortex Eucommiae 4-5 part, Radix Codonopsis 2-4 part, Radix Angelicae Sinensis 3-5 part, Flos Lonicerae 6-8 part, Radix Bupleuri 9-10 part, Fructus Mume 3-5 part, Pericarpium Granati 4-6 part, Fructus Schisandrae Chinensis 9-10 part.
2. mix preparation according to claim 1, it is characterized in that, in described mix preparation, the weight portion of Bacillus pumilus and immunostimulant is respectively: Bacillus pumilus 16 parts, vitamin C 12 parts, the Radix Astragali 8 parts, the Cortex Eucommiae 5 parts, Radix Codonopsis 4 parts, Radix Angelicae Sinensis 5 parts, Flos Lonicerae 8 parts, Radix Bupleuri 10 parts, Fructus Mume 5 parts, Pericarpium Granati 6 parts, Fructus Schisandrae Chinensis 10 parts.
3. according to the arbitrary described mix preparation of claim 1-2, it is characterized in that, the preparation method of described mix preparation is that described Bacillus pumilus is made microbial bacterial agent through liquid fermentation or solid fermentation, and in described microbial bacterial agent, Bacillus pumilus viable count is 10
9-10
11cfu/g, after described microbial bacterial agent, vitamin C, the Radix Astragali, the Cortex Eucommiae, Radix Codonopsis, Radix Angelicae Sinensis, Flos Lonicerae, Radix Bupleuri, Fructus Mume, Pericarpium Granati and Fructus Schisandrae Chinensis were pulverized 100 mesh sieves respectively, according to weight mixing, obtains described mix preparation.
4. according to the arbitrary described mix preparation of claim 1-2, it is characterized in that, described mix preparation uses as feeding additive aquatic animal or aquaculture water body additives.
5. mix preparation according to claim 4, is characterized in that, described Aquatic product is freshwater fish.
6. mix preparation according to claim 4, is characterized in that, the addition of described mix preparation in feedstuff is 1%-3%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410751286.7A CN104523956A (en) | 2014-12-10 | 2014-12-10 | Mixed preparation of bacillus pumilus and immunopotentiator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410751286.7A CN104523956A (en) | 2014-12-10 | 2014-12-10 | Mixed preparation of bacillus pumilus and immunopotentiator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104523956A true CN104523956A (en) | 2015-04-22 |
Family
ID=52839712
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410751286.7A Pending CN104523956A (en) | 2014-12-10 | 2014-12-10 | Mixed preparation of bacillus pumilus and immunopotentiator |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104523956A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101480432A (en) * | 2008-11-19 | 2009-07-15 | 广东海洋大学 | Compound Chinese herbal medicine immunopotentiator for Rachycentron canadum |
| CN103525723A (en) * | 2013-09-24 | 2014-01-22 | 上海海洋大学 | Bacillus pumillus microbial preparation with quorum sensing system inhibiting effect |
-
2014
- 2014-12-10 CN CN201410751286.7A patent/CN104523956A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101480432A (en) * | 2008-11-19 | 2009-07-15 | 广东海洋大学 | Compound Chinese herbal medicine immunopotentiator for Rachycentron canadum |
| CN103525723A (en) * | 2013-09-24 | 2014-01-22 | 上海海洋大学 | Bacillus pumillus microbial preparation with quorum sensing system inhibiting effect |
Non-Patent Citations (2)
| Title |
|---|
| 张厚梅: "中草药在水生动物病害防治中的应用", 《安徽农学通报》 * |
| 温周瑞等: "中草药对水产动物免疫作用研究进展", 《水利渔业》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Samad et al. | Effects of dietary katuk (Sauropus androgynus L. Merr.) on growth, non-specific immune and diseases resistance against Vibrio alginolyticus infection in grouper Epinephelus coioides | |
| CN102696884B (en) | Composite feed additive product | |
| CN101530165A (en) | Animal feed composite microecologic agent | |
| CN104146182A (en) | Biological feed additive for preventing and treating diarrhoea of piggies | |
| CN103966124A (en) | Compound microecological preparation and additive, as well as premix thereof | |
| CN102657287A (en) | Compound feed addictive with bacteria and oxidation resisting functions | |
| CN106754551A (en) | A kind of bacterium amount lactobacillus preparation high and preparation method and application | |
| CN110787247B (en) | Traditional Chinese medicine microecological preparation for preventing and treating livestock and poultry diarrhea and preparation method thereof | |
| CN102669435A (en) | Feed additive product | |
| CN105029045A (en) | Additive for aquatic feed | |
| WO2008023580A1 (en) | Animal feed additive | |
| CN103320365A (en) | Fish-sourced aeromonas hydrophila disease antagonistic strain and application thereof | |
| CN104855684A (en) | An antibacterial traditional Chinese medicine microbial fermentation preparation and a preparation method and application thereof | |
| CN104381697A (en) | Microecological preparation used in prawn feeds and application thereof | |
| WO2007034627A1 (en) | Additive for animal feed | |
| Qian et al. | Development and evaluation of a herbal formulation with anti-pathogenic activities and probiotics stimulatory effects | |
| CN105166487A (en) | Composite Chinese herbal medicine feed additive for pigs and preparation method of composite Chinese herbal medicine feed additive | |
| CN102367416A (en) | Traditional Chinese medicine probiotic solid culture medium for cow feed, culture method and feed additive | |
| CN103232959A (en) | Preparation method and application of feeding bacillus licheniformis inoculant | |
| CN102763772B (en) | Functional feed additive | |
| CN104286419A (en) | Novel feed additive | |
| CN101874812B (en) | Composite bacteria preparation and application thereof in weight increment of immature livestock and control of diarrhea | |
| CN102987114B (en) | Feed additive for live pig breeding | |
| Allinson et al. | Effect of herbal supplement on growth response and faecal egg counts of cockerels | |
| CN104399074A (en) | Application of bacillus pumilus and immunopotentiator mixed preparation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150422 |