CN104497094A - Processing method based on comprehensive utilization of stems and roots of cucurbitaceae melon plants - Google Patents

Processing method based on comprehensive utilization of stems and roots of cucurbitaceae melon plants Download PDF

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CN104497094A
CN104497094A CN201410832120.8A CN201410832120A CN104497094A CN 104497094 A CN104497094 A CN 104497094A CN 201410832120 A CN201410832120 A CN 201410832120A CN 104497094 A CN104497094 A CN 104497094A
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curcurbitaceae
root
comprehensive utilization
working method
mellon plant
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CN104497094B (en
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张奎昌
张志年
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Jiangsu Qianyaotang Traditional Chinese Medicine Research Institute Co Ltd
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Jiangsu Qianyaotang Traditional Chinese Medicine Research Institute Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07J—STEROIDS
    • C07J63/00—Steroids in which the cyclopenta(a)hydrophenanthrene skeleton has been modified by expansion of only one ring by one or two atoms
    • C07J63/008—Expansion of ring D by one atom, e.g. D homo steroids
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F3/00—Fertilisers from human or animal excrements, e.g. manure
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

The invention relates to a processing method based on comprehensive utilization of stems and roots of cucurbitaceae melon plants. The processing method comprises the following steps: boiling and leaching stems and roots of cucurbitaceae melon plants by using alkaline water as a solvent, acidifying a leachate to form a low-water-solubility bryonolic acid precipitate, separating, washing, recrystallizing and refining to obtain a bryonolic acid product; after performing drying, ultra-fine pulverization and enzymolysis treatment on leached waste residues, adding a moisture adjustment material, sterilizing, adding compound bacteria accounting for 8-12% by weight of a base material, fermenting, drying at a low temperature to obtain a biological protein feed product with a grain size of 80-90 meshes; pulverizing the waste residues, using 20-40% by weight of stem and root residue powder, 50-70% by weight of animal manure, and 10-15% by weight of saccharomycetes, fermenting, placing for 1 week at a temperature of 75-90 DEG C and humidity of 85-95%, drying, pulverizing, mixing 92.2-95.2% by weight of fermented material powder and 4.8-7.8% of compound microbial flora, and distributing, granulating, screening and packaging to obtain a bio-organic fertilizer product according to the conventional method.

Description

Based on the working method that Curcurbitaceae mellon plant stem and root fully utilize
Technical field
The invention belongs to extracted form natural plant field, relate to a kind of extracting method of bryonia alcohol acid.Particularly relate to the working method of Curcurbitaceae mellon plant stem and root comprehensive utilization, the stem of mellon plant and root is specifically utilized to extract bryonia alcohol acid, the stem of the mellon plant after then being extracted and the solid slag of root, obtain biological protein feedstuff product and biological organic fertilizer product further respectively.
Background technology
Bryonia alcohol acid (Bryonolic Acid, BA, also known as 3-Hydroxy-8-multifloren-29-oicacid).Chemistry 3-Hydroxy-8-multifloren-29-oicacid by name.
Structural formula is:
Bryonia alcohol acid is from Curcurbitaceae (Cucurbitaceae): red bryony Bryonia dioica root; The one extracted in black fruit bryonia Bryonia melanocarpa root acid friedooleanane type three note based compound.It is documented, bryonia alcohol acid is extensively present in Curcurbitaceae (Cucurbitaceae): wax gourd (Benincasa cerifera) root; Different bryonia Bryonia dioica root; Black fruit bryonia Bryonia melanocarpa root; Watermelon Citrullus lanatus root; Snake melon Cucumis melo var.conomon root; Muskmelon Cucumis melo root; Cucumber Cucumis sativus root; Buffalo gourd Cucurbita foetidissima root; Cucurbit Lagenaria siceraria root; Sponge gourd Luffa cylindrica; Trichosanthes bracteataVoigy Trichosanthes bracteata root; Hubei snakegourd Trichosanthes hupehensis root; Trichosanthes japonica Regel Trichosanthes kirilowii var.japonica root; Snakegourd Trichosanthes kirilowii root and Meliaceae (Meliaceae): India mountain pass chinaberry Sandoricum indicum; Datiscaceceae (Tetramelaceae): Tetrameles nudiflora Tetrameles nudiflora skin, leaf; Vitaceae (Vitaceae): in the plants such as radix ampelopsis Ampelopsis japonica root is wherein abundant with content in the root of the mellon plants such as Curcurbitaceae watermelon (Citrullus lanatus), snake melon (Cucumis melo var.conomon), pumpkin (Cucurbita moschata), muskmelon (Cucumis melo), cucumber (Cucumis sativus), sponge gourd (Luffa cylindrica) and stem.Very abundant in china natural resources with regard to above-mentioned mellon plant, there is very large DEVELOPMENT PROSPECT.
The medicinal effect that bryonia alcohol acid is given prominence to the most shows on anti-allergic effects, and it not only has the anti-allergic effects almost identical with glycyrrhetinic acid (GA), and shows than the irritated pawl of the suppression mouse ear property the touched IV type of strong several times of GA in effect; In addition, to the mouse toes swelling that histamine, thrombotonin or bradykinin cause, BA shows the inhibition of 10 times or more than 10 times stronger than GA.BA is different from GA, does not embrace the metabolism acyl activity (BASK) (Liu Tingting field 2014) of steroid hormone processed completely.
Because bryonia alcohol acid has antianaphylaxis, moisturizing, promotion dermal cell growth, check melanin cell enlargement, the multiple efficacies such as antitumor, also can be used as the raw material of other reactive derivatives of synthesis simultaneously, become the focus of attention of field of medicaments gradually.At present, in Japan, bryonia acid alcohol has been widely used in the fields such as medicine, makeup, food, and has carried out corresponding patent protection (Liu Tingting field 2014).
With regard to the development of current bryonia alcohol acid production technology, have scholar to contrast to conduct in-depth research: the comparatively ripe bryonia alcohol acid production technique mainly organic solvent extraction existed in prior art both at home and abroad, cellulase/extraction with aqueous solution method and biological synthesis process.
Organic solvent extraction.Because bryonia alcohol acid is mainly present in the root of the plant of Curcurbitaceae, Meliaceae and Vitaceae etc. and stem, therefore traditional bryonia alcohol acid is the root being taken out above-mentioned plant by organic solvent extracting, is then separated by chromatographic column and obtains.For traditional extraction process, want to improve productive rate when producing in enormous quantities, key factor is to process material of vegetable origin, extract the choosing of reagent, chromatographic column and the selection of chromatogram liquid and the function influence of other factors.Material of vegetable origin is processed, the people such as EMILY (Emily C.Barker) follow the trail of the enrichment content determining BA bryonia alcohol acid in pumpkin particular organization by HPLC, finally find that suitable position is the root of seedling and seedling, also investigate different cultivation duration simultaneously, culture medium on the impact of final BA content, thus determined the top condition of pumpkin raw material disposal.Extracting the selection of reagent, for extracting the extraction reagent of plant root based on polar organic reagent, comprising sherwood oil, chloroform, methyl alcohol etc., its Semi-polarity moderate based on chloroform, extraction reagent corresponding to different plant varieties is different.The selection of chromatographic column and chromatogram liquid, because the material extracted in every kind of plant has multiple, the main mode of chromatographic separation that adopts isolates bryonia alcohol acid cut at present.Chromatographic column adopts conventional silica gel chromatographic column usually, the proportioning of the selection of chromatogram liquid mainly sherwood oil, acetone, chloroform etc.In addition, mitsui petrochemical industry finds that the content of 2,4 dichlorophenoxyacetic acid in reduction system is to 5 × 10 -8mol/L can improve the productive rate of bryonia alcohol acid greatly.
Cellulase/extraction with aqueous solution method.A kind of preparation method's (publication number: CN102002087A) preparing bryonia alcohol acid of Nanjing Ze Lang Medicinal invention, get Curcurbitaceae mellon plant stem and root, add the water of its quality 5 times amount, add cellulase, temperature 40 DEG C to 50 DEG C, pH value 3 to 6, under enzyme amount 20U/g to 40U/g condition, enzymolysis 2 is little of 5 hours, be heated to seethe with excitement and decoct 0.5 little of 1.5 hours, filter, get filtrate, pass through absorption with macroporous adsorbent resin, with 10% to 50% ethanol elution, be collected into 8 times amount column volume elutriants, adsorbed by strong-basicity styrene type anionite-exchange resin, with the NaOH solution wash-out of 0.5mol/L, collect wash-out, filter, by strongly acidic styrene's type Zeo-karb, collect effluent liquid, and filter, filtrate is concentrated by reverse osmosis membrane, add methanol crystallization, fractional crystallization, washing, be drying to obtain.Its improvement is to substituted for traditional organic extract liquid with water and cellulase system, replaces organic solution as elutriant with strong base solution, and corresponding with strongly acidic styrene's Zeo-karb substituted for silicon gum resin.The method have been abandoned in previous methods conventional organic solvent and has repeatedly been processed caused seriously polluted, the defect such as energy consumption is large.
Biological synthesis process.The people such as Ta Bata (M.Tabata) disclose a kind of method watermelon culturing cell being prepared bryonia alcohol acid by biological synthesis process.It is by R [2- 14c]-mevalonic acid ion or [2- 14c]-acetic acid ion adds in watermelon culturing cell extraction liquid at ATP, Mg 2+ and NADPH exist under carry out cell cultures thus biosynthetic.In reaction process, according to the locating and displaying of tracer element, R [2- 14c] first-mevalonic acid ion be formed as 2,3-oxidosqualene, then under the effect of oxidasic disorganization, generate intermediate product isomultiflorenol with watermelon culturing cell, finally on the C-29 position of isomultiflorenol, methyl group oxidation forms acid thus obtains bryonia alcohol acid material (Liu Tingting field 2014).
As can be seen from above-mentioned summary Journal of Sex Research, the extraction at present with regard to bryonia alcohol acid adopts polar organic solvent mostly, by it from containing lixiviate the cucurbitaceous plant of bryonia alcohol acid out, is then separated by chromatographic column and obtains.But to leach in the crude product come not only containing a large amount of plurality of impurities such as vegetable polysaccharides, pigment, flavonoid, terpene, glycoside, phenolic acids, tartrate, tannin, also there is following shortcoming: 1. use multiple organic solvent, and consumption of organic solvent is large simultaneously; 2. operation is many, and just extraction one step generally needs through triple-stage cross-flow extraction, the loaded down with trivial details complexity of operation; 3. need repeatedly to distill, heat-up time is long; 4. active constituent content and leaching yield lower.Chinese patent (CN102002087A) discloses a kind of preparation method of bryonia alcohol acid, the technical scheme of this invention as previously mentioned, adopt cellulase/extraction with aqueous solution again through absorption with macroporous adsorbent resin, with 10% to 50% ethanol elution, be collected into 8 times amount column volume elutriants, by the absorption of strong-basicity styrene type anionite-exchange resin, with the NaOH solution wash-out of 0.5mol/L, collect elutriant, filter, effluent liquid is collected by strongly acidic styrene's type Zeo-karb, and filter, filtrate is concentrated by reverse osmosis membrane, add methanol crystallization, fractional crystallization, washing is drying to obtain.It improves and is to replace organic extract liquid with water and cellulase system, and corresponding with strongly acidic styrene's type Zeo-karb substituted for silicon gum resin, the organic solvent conventional in previous methods of having abandoned of program novelty processes caused seriously polluted, the defect such as energy consumption is large repeatedly.But also there is the numerous and diverse operation of multiple tracks that need process respectively anions and canons.Buck lixiviation process is utilized to be dissolved out from the rhizome system of cucurbitaceous plant by bryonia alcohol acid, make it to precipitate by the method for regulator solution pH again and obtain the character that is separated, be used for being separated the bryonia alcohol acid in leach liquor, for this reason, the applicant conducts in-depth research with regard to the method, through repetition test, finally obtain promising result, through literature search, there is not yet and just utilize buck lixiviate to be dissolved out by cucurbitaceous plant rhizome system, make it to precipitate by the method for regulator solution pH again and obtain the character of separation, be used for the relevant report of the bryonia alcohol acid be separated in leach liquor and patent application.
According to existing reported in literature, the bryonia alcohol acid extract yield in Curcurbitaceae mellon plant stem and root, because of kind difference slightly difference, but the average yield of just comprehensive extraction is between 0.2-0.7%, and if watermelon root yield is 0.195%, Radix Melo yield is 0.69%.But in the prior art, extract the waste residue after bryonia alcohol acid through the stem to mellon plant, root, then abandoned by as waste, this not only gives environment, and causes waste to resource.But the stem of Curcurbitaceae mellon plant and root all as medicinal material application, have history for a long time in China, as Towel Gourd Stem and root decoct juice or alcohol extract has cough-suppressing phlegm-dispelling functions and antibacterial and anti-inflammation functions." rattan and root, control that tooth is hidden, brain leaks, desinsection detoxifies " Compendium of Material Medica; " and blood vessels, grain of living, grows water, only cloudy pain, bowl spares invigorating the spleen, consumer edema.Control blood depletion few, waist knee numbness of the limbs, postpartum infantile convulsion, menstruation regulating " " book on Chinese herbal medicine asks former "; " solution hot summer weather " " south of the Five Ridges gather medicinal herbs record ".As Cushaw stem " sweet-bitter flavor, cold nature, nontoxic ", " entering liver, spleen two warp ", there is " sharp blood vessels, kidney nourishing water, control liver wind, and blood nourishes blood, menstruation regulating is regulated the flow of vital energy, and doublely removes all wind for suppressing the hyperactive liver and easing the stomach, the meridian dredging " effect " book on Chinese herbal medicine is new again "; " herbal medicine is commonly used in Shanghai ": " heat-clearing.Control pulmonary tuberculosis low-heat ".Among the peoplely pumpkin, the tender rattan of sponge gourd being stir-fried and eaten as dish, to cook soup etc. edible also very general, existing using Cushaw stem as the exploitation of fashion vegetable variety, having defined market climate, also arises at the historic moment in its specialty plantation family thereupon.
The pharmaceutical use of Curcurbitaceae mellon plant stem and root is very high, and edible nourishing enriches, the multiple nutrients materials such as melon stem and root fiber-enriched, starch, protein, amino acid, VITAMIN and mineral substance.Wherein amino acid is in the majority with arginine, citrulline; VITAMIN as B1, B2, also has carotene, vitamin A, vitamins C, nicotinic acid based on vitamin B group; Containing macro-and trace-element such as calcium, phosphorus, potassium, iron, zinc, selenium in mineral substance; In addition, also containing the benefit materials such as saponins material, amaroid, lymphatic temperament, trigonelline, VITAMIN B4, glucose, N.F,USP MANNITOL, piperylene, pectin, xylan and Interferon, rabbit and particular matter, there is certain special role.
Mellon plant stem and root processing is adopted to extract bryonia alcohol acid for the present invention, its extraction yield only accounts for the 0.2-0.7% of stem and root, if the solid slag being greater than 90% can not get adequately and reasonably applying, not only cause pollution to environment, also a large amount of wastings of resources will be caused simultaneously, for this reason, another object of the inventive method utilizes mellon plant stem and the solid slag of root after extracting bryonia alcohol acid further to process obtained biological protein feedstuff product; An object is also to utilize mellon plant stem and the solid slag of root after extracting bryonia alcohol acid further to process obtained biological organic fertilizer product again, realize the comprehensive processing and utilization to mellon plant stem and root, reach the comprehensive utilization effect of " eating dry bleeding ", promote " resource-conserving, environmentally friendly " socio-economic development.Solid slag after just utilizing Curcurbitaceae mellon plant stem and root processing to extract bryonia alcohol acid prepares biological protein feedstuff product and biological organic fertilizer product further, there is not yet relevant reported in literature and patent application through retrieval.
Summary of the invention
The object of this invention is to provide a kind of extracting method of bryonia alcohol acid.The open object of the present invention program overcomes the following shortcoming existed in prior art: 1. use multiple organic solvent, and the consumption of organic solvent is large; 2. operation is many, the loaded down with trivial details complexity of technique; 3. need repeatedly to distill, heat-up time is long; 4. active constituent content and leaching yield low etc.
Technical scheme of the present invention adopts buck to leach ratio juris, utilize bryonia alcohol acid can form phenates with some mineral alkali, basic salt and be dissolved out from system under certain condition, make it to precipitate and the character that is separated by the method for regulator solution pH again, the bryonia alcohol acid be used in separation leach liquor.The present invention adopts the program to carry out the leaching of bryonia alcohol acid, achieves good effect.
The detection method of the inventive method gained bryonia alcohol acid, the detection technique method disclosed in Chinese patent CN102002087A that have employed completely detects, that is: test example 1HPLC method measures bryonia alcohol acid purity
Chromatographic condition
Chromatographic column: octadecylsilane bonding glue silica gel is weighting agent; Moving phase: methyl alcohol-acetonitrile-water (8:10:82); Flow velocity: 1ml/ minute; Determined wavelength; 260nm; Column temperature: 30 DEG C.
Measuring method: precision takes bryonia alcohol acid 2mg, is placed in 50mL measuring bottle, and add methyl alcohol 20mL, sonic oscillation makes dissolving, and methanol constant volume, to scale, draws 10 μ L, injects high performance liquid chromatograph, adopts normalization method working sample purity.Its result UV, IR, MS, 2hNMR, 13the data that CNMR etc. characterize its physical behavior are consistent with prior art.
Technical scheme of the present invention is as follows:
Based on the working method that Curcurbitaceae mellon plant stem and root fully utilize, according to the present invention, following steps obtain bryonia alcohol acid product:
(1) get Curcurbitaceae mellon plant stem and root, pulverize with pulverizer, cross 50-60 mesh sieve, obtain meal;
(2) in step (1) meal, 40-50 DEG C is added with pure water through 0.1molL -1naOH regulates pH to be the leaching solvent of 10.2-12, and the weight ratio of meal and solvent is 1:(4-15), rotating speed is set under the constant speed, uniform stirring of 45r/min, is incubated 40-50 DEG C of hot dipping 1-4h;
(3) by the material after step (2) hot dipping, then rotating speed is adjusted under the constant speed, uniform stirring of 25r/min, heated and boiled 1.5-3h;
(4) material after step (3) being boiled is placed and is cooled to 40-45 DEG C; Then squeeze, obtain pressed liquor and filter residue;
(5) step (4) filter residue is added with pure water through 0.1molL -1naOH regulates pH to be the leaching solvent of 10.2-12, and the amount adding solvent is meal: the weight ratio of solvent is 1:3-10; Rotating speed is set under the constant speed, uniform stirring of 25r/min, heated and boiled 1-2h;
(6) material after step (5) being boiled is placed and is cooled to 40-45 DEG C, then squeezes, obtains pressed liquor and filter residue; Filter residue is for subsequent use;
(7) pressing filtering liquid step (4) and step (6) obtained carries out centrifugation, centrifugal rotational speed 5000-10000r/min, obtains supernatant liquor and solid phase precipitation thing after merging; Solid phase precipitation thing is for subsequent use;
(8) supernatant liquor that step (7) obtains is carried out essence filter, obtain smart cleaner liquid;
(9) smart cleaner liquid 0.5molL step (8) obtained -1hydrochloric acid soln regulates pH to 2.5-3.2, places 15min, when stablizing unchanged to pH value of solution, under putting 2-5 DEG C of environment, leaves standstill 20h, makes bryonia alcohol acid precipitate completely;
(10) step (9) bryonia alcohol acid is precipitated through 3000-3500r/min centrifugation, washing, refine with ordinary method recrystallization, vacuum-drying, obtain bryonia alcohol acid product.
The present invention obtains the method for bryonia alcohol acid, utilize the bryonia alcohol acid character that salify and solubility property increase in buck, be that solvent boils leaching with buck, after buck leach liquor is acidified with acid, the phenates of bryonia alcohol acid changes into the bryonia alcohol acid of low water solubility again, thus the precipitation forms of bryonia alcohol acid is separated from solution; Its principle is that bryonia alcohol acid belongs to polyatomic phenol material, has slightly acidic.In its structure, phenolic hydroxyl group quantity is many, and thus buck solubility is good; In bryonia alcohol acid structure, phenolic hydroxyl group polarity is relatively little, and thus in water, solubleness is less.But in the basic conditions, phenolic hydroxyl group can be transformed into salt and make water-soluble remarkable increase.Compared with organic solvent lixiviation process, alkali leaching method has the following advantages: (1) is simple for process, easy-to-operate, and its buck and sour water waste liquid are through being neutralized into Ficus caricaL, and unharmful substance discharges, and can not cause environmental pollution; (2) do not need to use a large amount of organic solvents, can production cost be reduced; (3) selectivity is strong, thus active constituent content and leaching yield high.Bryonia alcohol acid after extraction and isolation is through washing, then adopt processes well known recrystallizing and refining to obtain bryonia alcohol acid product that purity is >=95.6%.The inventive method, through the multiple batches of leaching carrying out bryonia alcohol acid, achieves good effect.
Following steps are continued, obtained biological protein feedstuff product after above-mentioned steps (6):
(11) the solid phase precipitation thing that filter residue step (6) obtained and step (7) obtain merges, and under 60 DEG C of-85 DEG C of conditions, dry 24-36 hour, obtains dry slag;
(12) the dry slag of step (11) gained is cooled to room temperature, pulverizing, the dry slag after pulverizing crosses 250-350 mesh sieve, obtains mellon plant stem root ground-slag; Meal returns to be pulverized again;
(13) pure water adding 40-45 DEG C inserted in cultivation and fermentation tank by mellon plant stem root ground-slag step (12) obtained, and the weight ratio of mellon plant stem, root ground-slag and pure water is 1:(2-10), soak 1-2h, composite slip;
(14) in step (13) gains slip, add the cellulase (unit of activity is 2000U/g) of weight 0.3-0.6%, adjustment pH is 4-5.5, and be heated to 40-50 DEG C, insulation 5-14h, period carries out stirring 10min every 30min, obtains enzymolysis slip;
(15) step (14) gained enzymolysis slip is cooled to room temperature to release, in enzymolysis slip, adds moisture adjustment material, the moisture of whole material is adjusted to 55-65%w/w, makes solid medium, then culture base-material is carried out sterilizing;
(16) by the solid culture base-material after step (15) sterilizing through naturally cooling to below 45 DEG C time, add culture base-material weight 8-12% composite bacteria, mixing material, load in Stainless Steel Disc, bed thickness is 4-6 centimetre, in immigration proving room, be placed on fermenting frame by fermented product charging tray, regulation and control room temp is 25-36 DEG C, room ventilated amount is 0.8m 3under/min condition, solid-state ventilating fermentation cultivates 30-50 hour, obtains fermentation culture material;
(17) by the fermentation culture material that step (16) obtains, low-temperature vacuum drying or lyophilize is adopted;
(18) by step (17) dried material, through pulverizing 100 mesh sieves, adopt dry granulation machine to be directly compressed into particle, the grain diameter be pressed into was 80-90 order, through metering, packaging, namely became biological protein feedstuff of the present invention.
The biological protein feedstuff that aforesaid method obtains has following characteristic: product is mealiness particulate matter, water content lower than 6%, composite bacteria living bacteria count is not less than 1,000,000,000/gram, protein content is at 32-38%w/w, organic content is 42-58%w/w, detect department through feed to detect, reach national fodder production standard.
The solid phase precipitation thing that the filter residue obtain above-mentioned steps (6) and step (7) obtain merges after drying, further employing super-fine powder is broken into 250-350 order fine powder, effectively can promote the stripping of effective components in plants, improve bioavailability, be beneficial to the metabolic process of microbial bacteria fermentation, also can improve absorption and the availability of the finished product simultaneously.
Cellulase is further utilized to carry out enzymolysis, can be made vegetable fibre fully decompose, destroy cell walls, increase the stripping of vegetable cell content, can promote effective ingredients in plant content, cellulose decomposition can also be glucose by cellulase, improves the nutritive value of feeds product.
Further employing composite bacteria of the present invention can produce the multiple enzyme of such as proteolytic enzyme, lipase, amylase, chitinase etc.; Produce vitamin B group; Form mycoprotein; Can be micromolecular polypeptide and oligosaccharides by macro-molecular protein, amino acid and the fat acid decomposition in mellon plant and adjustment material, the protein content of dish dregs of rice class can also be brought up to 12% from 8%.
Become or lyophilize through terminal low-temperature vacuum drying further, product water content is made to be less than 5%, product is made 80-90 order powdered granule further, be easy to flowing, mix feed uniformity coefficient, be also beneficial to the stable of microbial bacteria gemma, microorganism is made to be in dormant state, retain the quantity of microorganism live bacteria to greatest extent, and be beneficial to storage, be convenient to transport, its biologic live bacteria can be bred rapidly and played a role under suitable growing environment; Composite bacteria of the present invention is better than other single microbial bacteria feeds, and has stronger resistance, can breed rapidly under animal hydrochloric acid in gastric juice condition and anaerobic conditions, guarantees to enter in animal gastrointestinal tract and has an effect.Product of the present invention has the feature of high viable bacteria (composite bacteria living bacteria count be not less than 1,000,000,000/gram), high protein (> 32%), growth-promoting effect as biological protein feedstuff can substitute the microbiotic and growth promoter that add in feed, improves ecological benefits; Biological protein feedstuff can make an addition in feed in the ratio of 8-12% and substitute feed grain, such as corn, dregs of beans etc., saves food.The present invention solve simultaneously above-mentioned bryonia alcohol acid is extracted after mellon plant stem and the difficult problem of waste disposal of root, by enzymolysis, microbial transformation, this waste is converted into biological protein feedstuff, not only turn waste into wealth, reduce environmental pollution, economize on resources, the raising aspect such as livestock and poultry output and meat quality thereof is particularly useful, there is economic benefit and the social benefit of significant comprehensive utilization.
After above-mentioned steps (6), continue following steps, biological organic fertilizer product can also be obtained:
(19) the solid phase precipitation thing that filter residue step (6) obtained and step (7) obtain merges, and dries 20-32 hour, obtain dry slag under 60-95 DEG C of condition;
(20) the dry slag that step (19) obtains is cooled to room temperature to pulverize, crosses 85-95 mesh sieve, obtain mellon plant stem root ground-slag, for subsequent use;
(21) mellon plant stem root ground-slag step (20) obtained mixes with animal excreta and saccharomycetes to make fermentation agent, and the adjustment water content that adds water is to 55-60%w/w, and be modulated into and treat fermentation solid medium material, wherein animal excreta is the animal excreta after drying and other treatment; Wherein the weight percent of mellon plant stem root ground-slag, animal excreta and saccharomycetes to make fermentation agent is: mellon plant stem root ground-slag 20-40%, animal excreta 50-70%, saccharomycetes to make fermentation agent 10-15% ratio;
(22) what step (21) modulated treats fermentation solid medium material, load in wooden or bamboo case, bed thickness is 20-25 centimetre, move in the wet proving room of controlled temperature control, first temperature be 29-34 DEG C, humidity be 60-80% condition under spontaneous fermentation 24-36 hour, then adjust room temp be 75-90 DEG C, humidity be under 85-95% condition place 1 week, obtain fermentation materials;
Temperature be 75-90 DEG C, humidity be 85-95% condition under place 1 week, object is that fermentation materials carries out Maillard reaction further, one is to make the albumen of fermentation materials be further converted to amino acid and amino acid short peptide and little peptide, promote fertilizer biologicak efficiency, in addition, be further maturation process is carried out to fermented product, can make that the effect of using of product is fast, fertility is strong.
(23) by the fermentation materials that step (22) obtains, under 60-90 DEG C of condition, carry out drying, make drying materials to water ratio 6% or lower;
(24) after the siccative that step (23) obtains being cooled to room temperature, pulverize, cross 60-80 mesh sieve, obtain fermentation materials powder;
(25) fermentation material powder step (24) obtained and composite microbial bacteria are fermentation material powder 92.2-95.2% in mass ratio, composite microbial bacteria 4.8-7.8% proportions, after mixing, method is routinely carried out preparing burden, granulation, screening, is namely obtained bio-organic fertilizer product after packaging.
The bio-organic fertilizer product obtained by the present invention carries out comprehensive detection by organic fertilizer NY884-2004 and composite microbiological fertilizer NY/T798-2004 standard respectively, obtains its technical indicator as shown in the table:
Table
Utilize the bio-organic fertilizer that the inventive method is obtained, the operational path of employing is not the thinking of offal treatment, but the thinking to resource circulation utilization.To environment without any pollution, more than 98% is reached to the utilization ratio of resource; And the index of bio-organic fertilizer product meets agricultural industry biological organic fertilizer standard-required.
The bio-organic fertilizer product that the inventive method is obtained, one of its main raw material is the waste after extraction bryonia alcohol acid by the stem of its mellon plant and root, i.e. stem, root slag mixes with the animal excrement after drying and other treatment, utilizes saccharomycetes to make fermentation, then again through Maillard reaction, adds mixed bacterium after drying again through mixing, granulation, dry, packaging is made, through saccharomycetes to make fermentation can be useful the organic composition of material is transformed, increase amino acid especially, the content of vitamin B group, containing abundant VITAMIN in yeast, amino acid, protein, nucleic acid, inorganics, sugar, crude fat etc. by the culture material after yeast bio-transformation, then are 75-90 DEG C through temperature, humidity is that the environment of 85-95% is placed 1 week, its objective is and make fermentation materials carry out Maillard reaction further, one is effectively to make the protein component in fermentation materials be converted into amino acid, making amino acid converting is little peptide or small peptide, makes to be obtained by the protein that plant directly utilizes useful conversion, promotes the biologicak efficiency of fertilizer, improve result of use, in addition be carry out maturation process to fermented product further, the effect of using of product can be made fast, and fertility is strong.
Organic content through technical scheme process artifact organic fertilizer of the present invention is high, can reach more than 75%.Simultaneously obtained bio-organic fertilizer is nutritious, total Readily oxidized organic matter content reaches 22-24%, and containing rich in protein, amino acid, carbohydrate, vitamin B group, mycoprotein, mineral trace element, be particularly suitable for the fertilizer in green planting production.
According to the present invention, do to optimize further and/or select to technique scheme:
Curcurbitaceae mellon plant described in above-mentioned steps (1) is sponge gourd (Luffa cylindrica), cucumber (Cucumis sativus), watermelon (Citrullus lanatus), pumpkin (Cucurbita moschata), wax gourd (Benincasa cerifera), muskmelon (Cucumis melo) and snake melon (Cucumis melo var.conomon).
Stem and the root of the sponge gourd in Curcurbitaceae mellon plant described above, cucumber, watermelon, pumpkin, wax gourd, muskmelon and snake melon are dry product or fresh goods; Advantageous applications is dry product.
In above-mentioned steps (2), the weight ratio of preferred meal and solvent is 1:(5-10).
Insulation 40-50 DEG C of hot dipping time preferred 1.5-2.5h in above-mentioned steps (2); Insulation hot dipping for some time can increase soluble components stripping.
The preferred pH of leaching solvent described in above-mentioned steps (2) is 10.2-10.5; Heated and boiled time preferred 1.5-2h in described step (3).
Packaging type squeezing machine is selected in above-mentioned steps (4) and the squeezing described in (6);
In above-mentioned steps (5), preferred pH scope is 10.2-10.5; Preferred meal: the weight ratio of solvent is 1:(3-5).
The pressed liquor centrifugation that is combined described in above-mentioned steps (7) preferably uses horizontal spiral centrifuge; Preferred centrifugal rotational speed is 6000-8000r/min.
The filtering with microporous membrane that essence filter described in above-mentioned steps (8) uses aperture to be less than 0.45 μm.
Vacuum-drying condition described in above-mentioned steps (10) is temperature 55-60 DEG C, vacuum tightness-0.085--0.1MPa.
Drying described in above-mentioned steps (11), preferred drying conditions is under 75-85 DEG C of condition, dry 30-36 hour.
Drying described in above-mentioned steps (11) adopts warm air drying or far infrared drying; Pulverizing described in step (12) is micronizing, preferably uses horizontal continuously stirring pulverizer.
The preferred weight ratio of the mellon plant stem root ground-slag described in above-mentioned steps (13) and water is 1:(3-5).
The preferred condition of enzymolysis described in above-mentioned steps (14) is pH is 4.0-4.4, temperature is 42-45 DEG C, the time is 8-12h.
Moisture adjustment material described in above-mentioned steps (15) is selected from wheat bran, rice bran, maize peel, brewer's grains, the dish dregs of rice, cotton dregs any one or its mixture; Preferred use maize peel, brewer's grains, the dish dregs of rice, cotton dregs or its mixture.
The preferred add-on of the moisture adjustment material described in above-mentioned steps (15) makes moisture content of material be down to 56-63%W/W.
Sterilizing in above-mentioned steps (15), uses hot pressing 0.7Kg/cm 2sterilizing in 115 DEG C, 35 minutes.
Composite bacteria described in above-mentioned steps (16) contains the mixed bacterium of following bacterial classification: yeast (Saccharomyces), genus bacillus (Bacillus) and milk-acid bacteria (Lactobacillus); By weight being yeast: genus bacillus: milk-acid bacteria=2:1:1 proportions mixes.
Yeast described above commercially available all of choice for use can be used as foodstuff additive or fodder additives and be every gram and be no less than Candida utilis (Candida utilis), any one in cereuisiae fermentum (Saccharomyces cerevisiae) or fodder yeast (Candida Tropicali) of 20,000,000,000 containing viable bacteria concentration; Preferred use fodder yeast (Candida Tropicali).
Genus bacillus (Bacillus) described above uses one or more arbitrary combination in subtilis (Bacillus subtilis), Bacillus licheniformis (Bacillus licheniformis), Bacillus coagulans (Bacillus coagulans), bacstearothermophilus (Bacillus stearothermophilus) and bacillus firmus (Bacillusfirmus).
Milk-acid bacteria (Lactobacillus) described above uses one or more arbitrary combination in thermophilus streptococcus (Streptococcus thermophilus), thermophilic lacto-bacilli (Lactobacillus thermophilus), plant lactobacillus (Lactobacillus plantarum), Lactococcus lactis (Lactococcus lactis), lactobacillus bulgaricus (Lactobacillus bulgaricus).
Genus bacillus (Bacillus) described above and milk-acid bacteria (Lactobacillus), commercially available all of choice for use can be used as foodstuff additive or fodder additives every gram are no less than 20,000,000,000 thalline dry powder goods containing viable bacteria concentration.
Every gram described above is no less than to yeast, genus bacillus and the milk-acid bacteria thalline dry powder goods of 200 hundred million containing viable bacteria concentration, except the edible or feeding viable bacteria goods that choice for use is commercially available, can also, according in the prior art conventionally known to one of skill in the art, be not unobtainable by understanding the growth properties of bacterium.As by each bacterial classification individually according to a conventional method from preserve the streak inoculation of bacterial classification (as cryopreservation) picking bacterial classification to solid plate substratum, cultivate under conditions suitable, bacterium colony to be grown, adopt liquid culture fermentation equipment to be inoculated in the liquid nutrient medium of corresponding optimization again and carry out shaking culture, can find, as handbook or the textbook of the cultivation about microorganism from relevant handbook as culture condition (as temperature etc.).
In order to be applicable to the fermentative processing to material of the present invention.Can be adopted liquid culture fermentation equipment in respective independently culture system by the substratum optimized, cultivate Candida utilis respectively, cereuisiae fermentum, fodder yeast and subtilis, Bacillus licheniformis, Bacillus coagulans, bacstearothermophilus, bacillus firmus and thermophilus streptococcus, thermophilic lacto-bacilli, plant lactobacillus, Lactococcus lactis, lactobacillus bulgaricus, then with supercentrifuge, the thalline in fermented liquid is separated, be placed in dry indoor cold air drying or vacuum lyophilization mode bacterium mud is dry and obtain, obtain the thalline dry powder goods that viable bacteria concentration is no less than every gram 20,000,000,000 respectively, can be used alone according to formula needs, also can proportionally carry out used in combination.
The preferred room temp of above-mentioned steps (16) is 29-33 DEG C; Preferred solid-state aerlbic culture 36-42 hour.
The condition of the low-temperature vacuum drying described in above-mentioned steps (17) is temperature 34-37 DEG C, vacuum tightness-0.09--0.1MPa; Described cryodesiccated condition is temperature-55--50 DEG C, vacuum tightness 25-35Pa.
In order to the stability making product assurance enough, preferably the above-mentioned culture material drying fermented is made moisture to material water ratio 6 or lower, microbial bacteria preferably is in dormant state, is of value to the storage of product.
Oven dry described in above-mentioned steps (19), preferred drying condition for dry 22-25 hour under 80-95 DEG C of condition; Described oven dry adopts hot-air seasoning.
Animal excreta in above-mentioned steps (21) is the mixture of herbivorous animal rabbit excrement, sheep excrement, cow dung, horsehit, donkey excrement, camel excrement or its arbitrary combination; Be preferably the mixture of rabbit excrement, sheep excrement, cow dung or its arbitrary combination.
Animal excreta drying and other treatment described in above-mentioned steps (21) carries out drying to water content < 5% through 135 DEG C for adopting dryer, applies after crossing 80 mesh sieves.
Drying in above-mentioned steps (23), preferred drying conditions is temperature 80-90 DEG C; Described drying adopts rotary dryer.
Composite microbial bacteria described in above-mentioned steps (25) is mixed in proportion by the milk-acid bacteria (Lactobacillus) of following bacterial classification, genus bacillus (Bacillus) and enzymatic microorganism and forms, and preferred weight part is than being milk-acid bacteria: genus bacillus: enzymatic microorganism=1:1:1 ratio.
Above-mentioned milk-acid bacteria selects one or more arbitrary combination in thermophilus streptococcus (Streptococcus thermophilus), streptococcus acidi lactici (Streptococcus acidi lactici), Lactobacterium acidophilum (Bacillus acidophilus), lactobacillus delbruckii (Lactobacillus delbriickii), Lactobacillus rogosae (Lactobacillus rentdril).
Genus bacillus described above uses one or more arbitrary combination in Bacillus licheniformis (Bacillus licheniform is), Bacillus circulans (Bacillus circulans Jordan), subtilis (Bacillus subtilis), bacillus firmus (Bacillusfirmus) and bacillus lentus (Bacillus lentus).
Milk-acid bacteria described above and genus bacillus are that every gram of viable bacteria concentration is no less than 200 hundred million thalline dry powder goods.
Milk-acid bacteria described above and genus bacillus can utilize the known bacterial classification of prior art completely, such as, at bacterial classification or other bacterial classification that can obtain from prior art of the preservation center preservation of Chinese Academy of Sciences microbe research common micro-organisms preservation center and other branch.Composite microbial bacteria of the present invention can single culture in advance, mixes during use again; Being no less than the milk-acid bacteria of 200 hundred million and the dry powder goods of genus bacillus for every gram of viable bacteria concentration described above, is not unobtainable according to existing known technology by understanding the growth properties of biological bacteria.As by each bacterial classification individually according to a conventional method from preserve the streak inoculation of bacterial classification (as cryopreservation) picking bacterial classification to solid plate substratum, cultivate under conditions suitable, bacterium colony to be grown up to, adopt liquid culture fermentation equipment again, be inoculated in the liquid nutrient medium of corresponding optimization and carry out shaking culture, can find, as handbook or the textbook of the cultivation about microorganism from relevant handbook as culture condition (as temperature etc.).
In order to be suitable for adding the stable of microbial bacteria in material of the present invention, ensure microbial bacteria effective survival rate in the product, can liquid culture fermentation equipment be adopted in respective independently culture system to cultivate thermophilus streptococcus respectively by the substratum optimized, streptococcus acidi lactici, Lactobacterium acidophilum, lactobacillus delbruckii, Lactobacillus rogosae and Bacillus licheniformis, Bacillus circulans, subtilis, bacillus firmus, bacillus lentus, then with high speed centrifugation, the thalline in fermented liquid is separated, be placed in dry indoor cold air drying or vacuum lyophilization mode, the drying of bacterium mud is obtained, obtain the thalline dry powder goods that viable bacteria concentration is no less than every gram 20,000,000,000 respectively, can be used alone according to formula needs, also can proportionally carry out used in combination.
The milk-acid bacteria that milk-acid bacteria described above and genus bacillus can also be applied in choice for use bio-feritlizer commercially available or feed and genus bacillus are no less than the thalline dry powder goods of 200 hundred million containing every gram of viable bacteria concentration.
Enzymatic microorganism described above use commercially available every gram to be no less than containing viable bacteria concentration 5,000 ten thousand dry powder goods.
The above equipment of the present invention is this area conventional equipment.
Do not state the operation of its technological process in processing step in the present invention in detail, be the routine operation in the general knowledge of those skilled in the art.
Except as otherwise noted, per-cent of the present invention is all weight percentage.
Technical scheme of the present invention has following beneficial effect:
1, produce bryonia alcohol acid with Curcurbitaceae mellon plant, be the main plant resource of bryonia alcohol acid, utilize new method development will make important contribution to the Sustainable development of industry.The present invention utilizes the stem of mellon plant and root to produce bryonia alcohol acid, and the processing technology routine of employing is not only the thinking of offal treatment but the thought fully fully utilized resource, to environment without any pollution, more than 98% is reached to the utilization ratio of resource.
2, fill up the domestic recycling utilizing mellon plant stem and root to produce bryonia alcohol acid and byproduct (waste material) and provide new Method and process, technological line.
3, technique of the present invention is with the method for potass extraction, Acid precipitation, compared with organic solvent, both reduced production cost, and turn avoid the pollution of residual solvent, the form that the alkaline waste water in step of the present invention and acid waste water form salt through neutralizing treatment can not pollute environment.
4, raw material is imitated utilization by technique of the present invention entirely, utilize mellon plant stem and root after extracting bryonia alcohol acid, utilize its stem, root slag passes through microbial transformation, be separately converted to biological protein feedstuff and/or bio-organic fertilizer product, do not produce waste, meeting and eat the dry processing principle bled to raw material, is environmentally friendly process for cleanly preparing.Technology of the present invention not only implements and utilizes melon stem and root effectively to extract bryonia alcohol acid, its mellon plant stem root is fully utilized simultaneously and further biological protein feedstuff and/or bio-organic fertilizer are converted into its stem root slag, not only to the exploitation of its bryonia alcohol acid high value added product, comprehensive utilzation is carried out to raw material resources simultaneously, not only turn waste into wealth, reduce environmental pollution, and biological protein feedstuff can also improve livestock and poultry output and meat quality thereof, realize the aspects such as nuisance free meat products to be particularly useful, bio-organic fertilizer product improves soil link to raising, improve crop yield, promote agriculture green planting to be even more important equally, there is significant economic and social benefit.
Embodiment
Below in conjunction with embodiment, the specific embodiment of the invention is further illustrated; as limitation of the present invention should not be regarded; to following preferred embodiment to one skilled in the art; under the prerequisite not departing from the present invention program's know-why, the improvements and modifications of carrying out all are considered as protection scope of the present invention.
Embodiment 1
A working method for Curcurbitaceae mellon plant stem and root comprehensive utilization, step is as follows:
(1) get Curcurbitaceae mellon plant towel gourd stem and root pulverizer is pulverized, cross 50-60 mesh sieve, obtain meal;
(2) taking the meal 100 kilograms that step (1) obtains is placed in extractor, with the pure water of 40-50 DEG C through 0.1molL -1naOH adjustment pH is the leaching solvent 1000 kilograms of 10.2-10.5, and arranging rotating speed is that 45r/min constant speed stirs, and passes into steam-heated cal(l)andria, make extractive substance temperature maintain the temperature at 40-50 DEG C of hot dipping 1.5h through extractor interlayer;
(3) by the material after step (2) hot dipping, adjustment rotating speed is that the constant speed of 25r/min stirs, and adjustment steam intake, is heated to 100 DEG C and boils 2h;
(4) material after step (3) being boiled is cooled to 40-45 DEG C; Then adopt packaging type squeezing machine to squeeze, obtain pressed liquor and filter residue;
(5) filter residue obtained after step (4) being squeezed, adds pure water through 0.1molL -1naOH adjustment pH is the leaching solvent 300 kilograms of 10.2-10.5, arranges rotating speed under 25r/min constant speed stirs, is heated to 100 DEG C and boils 1.5h;
(6) material after step (5) being boiled is placed and is cooled to 40-45 DEG C, squeezes, obtain pressed liquor and filter residue with packaging type squeezing machine; Filter residue is for subsequent use;
(7) pressed liquor that pressed liquor step (4) obtained and step (6) obtain merges, and uses horizontal spiral centrifuge centrifugation, centrifugal rotational speed 6000r/min, obtains supernatant liquor and solid phase precipitation thing; Solid phase precipitation thing is for subsequent use;
(8) supernatant liquor that step (7) obtains is adopted the filter of 0.42 μm of millipore filtration essence, obtain smart cleaner liquid;
(9) smart cleaner liquid 0.5molL step (8) obtained -1hydrochloric acid soln regulates pH to 2.5, places 15min, when stablizing unchanged to pH value of solution, under being placed in 2-5 DEG C of environment, leaves standstill 20h, makes bryonia alcohol acid precipitate completely;
(10) step (9) bryonia alcohol acid precipitation is used 3000r/min centrifugation, washing, with recrystallizing methanol carry out refining after, in temperature be 55 DEG C, vacuum tightness is drying to obtain colorless crystalline bryonia alcohol acid 758 grams under being 0.1MPa condition, detect through HPLC, purity is 95.6%.
Following steps are continued, obtained biological protein feedstuff product after above-mentioned steps (6):
(11) the solid phase precipitation thing that filter residue step (6) obtained and step (7) obtain merges, and insert in baking oven, under 75 DEG C of conditions, warm air drying 36 hours, obtains dry slag;
(12), after the dry slag of step (11) gained being cooled to room temperature, adopting horizontal continuously stirring to pulverize and carry out micronizing, cross 250 mesh sieves after pulverizing, obtain towel gourd stem, root ground-slag;
(13) take the obtained towel gourd stem of step (12), root ground-slag 100 kilograms inserts in cultivation and fermentation tank the pure water 300 kilograms adding 40-45 DEG C, soak 2h, composite slip;
(14) in step (13) gains slip, add the cellulase 1.25 kilograms that unit of activity is 2000U/g, adjustment pH is 4.0-4.4, stirs, adjustment material temperature to 43 DEG C, insulation 10h, and period carries out stirring 10min every 30min, obtains enzymolysis slip;
(15) step (14) gained enzymolysis slip is cooled to room temperature hypsokinesis to go out, (moisture adjustment material) maize peel 50 kilograms and the dish dregs of rice 50 kilograms are added in enzymolysis slip, the moisture content of material is adjusted to 60%, make solid medium, then by substratum autoclave sterilizer at hot pressing 0.7kg/cm 2, sterilizing 35 minutes under temperature 115 DEG C of conditions;
(16) by the solid culture base-material after step (15) sterilizing, when being cooled to below 45 DEG C, add the composite bacteria be made up of 22 kg diet yeast, 11 kilograms of subtilises and 11 kilograms of thermophilic lacto-bacillies, stirring and evenly mixing, load in Stainless Steel Disc, bed thickness is 4-6 centimetre, moves in proving room, be placed on fermenting frame by fermented product charging tray, regulation and control room temp is 29 DEG C, room ventilated amount is 0.8m 3/ min, solid-state ventilating fermentation cultivates 42 hours, obtains fermentation culture material;
(17) by the fermentation culture material that step (16) obtains, be placed in Vacuumdrier, under temperature 34 DEG C, vacuum tightness-0.1MPa condition vacuum-drying to material moisture 5.6%;
(18) by step (17) dried material, through pulverizing 100 mesh sieves, adopt dry granulation machine to be directly compressed into particle, the grain diameter be pressed into was 80-90 order, through metering packing, obtained biological protein feedstuff product.
Following steps are continued, obtained biological organic fertilizer product after above-mentioned steps (6):
(19) solid phase precipitation thing that filter residue step (6) obtained and step (7) obtain merges, and with baking oven hot-air seasoning 25 hours under 80 DEG C of conditions, obtains dry slag;
(20) after the dry slag that step (19) obtains being cooled to room temperature, pulverize with Universalpulverizer, cross 85-95 mesh sieve, obtain towel gourd stem, root ground-slag;
(21) get towel gourd stem, root ground-slag 40 kilograms that step (20) obtains, mix with 50 kilograms, rabbit excrement after mummification and saccharomycetes to make fermentation agent 10 kilograms of proportionings, by suitable quantity of water adjustment water content to 55-60%, make and wait the solid culture medium that ferments;
(22) what step (21) modulated waits the solid culture medium that ferments, load in wooden (-packing) case, bed thickness is 20 centimetres, move in the proving room of controlled epidemic disaster, first temperature be 29 DEG C, humidity be 60% condition under spontaneous fermentation 36 hours, and then adjust that room temp is 75 DEG C, humidity is 95% placement 1 week, obtain fermentation materials;
(23) by the fermentation materials that step (22) obtains, under 80 DEG C of conditions, carry out drying with rotary dryer, make drying materials be less than 6% to water ratio;
(24) after the siccative that step (23) obtains being cooled to room temperature, pulverize with Universalpulverizer, cross 60 mesh sieves, obtain fermentation materials powder;
(25) after 95.2 kilograms, fermentation materials powder step (24) obtained and thermophilus streptococcus, Bacillus licheniformis and enzyme element bacterium mix in composite microbial bacteria 4.8 kilograms of proportionings that 1:1:1 ratio forms, carry out preparing burden, namely granulation, screening, packaging obtain bio-organic fertilizer product.
Embodiment 2
A working method for Curcurbitaceae mellon plant stem and root comprehensive utilization, step is as follows:
(1) get Curcurbitaceae mellon plant Cucumis sativus stem and root pulverizer is pulverized, cross 50-60 mesh sieve, obtain meal;
(2) taking the meal 100 kilograms that step (1) obtains is placed in extractor, with the pure water of 40-50 DEG C through 0.1molL -1naOH adjustment pH is the leaching solvent 500 kilograms of 10.2-10.5, and arranging rotating speed is that 45r/min constant speed stirs, and passes into steam-heated cal(l)andria, make extractive substance temperature maintain the temperature at 40-50 DEG C of hot dipping 2h through extractor interlayer;
(3) by the material after step (2) hot dipping, adjustment rotating speed is that the constant speed of 25r/min stirs, and adjustment steam intake, is heated to 100 DEG C and boils 2.5h;
(4) material after step (3) being boiled is cooled to 40-45 DEG C; Then adopt packaging type squeezing machine to squeeze, obtain pressed liquor and filter residue;
(5) filter residue obtained after step (4) being squeezed, adds pure water through 0.1molL -1naOH adjustment pH is the leaching solvent 500 kilograms of 10.2-10.5, arranges rotating speed under 25r/min constant speed stirs, is heated to 100 DEG C and boils 1.5h;
(6) material after step (5) being boiled is placed and is cooled to 40-45 DEG C, squeezes, obtain pressed liquor and filter residue with packaging type squeezing machine; Filter residue is for subsequent use;
(7) pressed liquor that pressed liquor step (4) obtained and step (6) obtain merges, and uses horizontal spiral centrifuge centrifugation, centrifugal rotational speed 8000r/min, obtains supernatant liquor and solid phase precipitation thing; Solid phase precipitation thing is for subsequent use;
(8) supernatant liquor that step (7) obtains is adopted the filter of 0.22 μm of millipore filtration essence, obtain smart cleaner liquid;
(9) smart cleaner liquid 0.5molL step (8) obtained -1hydrochloric acid soln regulates pH to 3.0, places 15min, when stablizing unchanged to pH value of solution, under being placed in 2-5 DEG C of environment, leaves standstill 20h, makes bryonia alcohol acid precipitate completely;
(10) step (9) bryonia alcohol acid precipitation is used 3500r/min centrifugation, washing, with recrystallizing methanol carry out refining after, in temperature be 60 DEG C, vacuum tightness is drying to obtain colorless crystalline bryonia alcohol acid 574 grams under being 0.085MPa condition, detect through HPLC, purity is 96.3%.
Following steps are continued, obtained biological protein feedstuff product after above-mentioned steps (6):
(11) the solid phase precipitation thing that filter residue step (6) obtained and step (7) obtain merges, and insert in baking oven, under 85 DEG C of conditions, warm air drying 30 hours, obtains dry slag;
(12), after the dry slag of step (11) gained being cooled to room temperature, adopting horizontal continuously stirring to pulverize and carry out micronizing, cross 300 mesh sieves after pulverizing, obtain Cucumis sativus stem, root ground-slag;
(13) take the obtained Cucumis sativus stem of step (12), root ground-slag 100 kilograms inserts in cultivation and fermentation tank the pure water 400 kilograms adding 40-45 DEG C, soak 1h, composite slip;
(14) in step (13) gains slip, add the cellulase 2.5 kilograms that unit of activity is 2000U/g, adjustment pH is 4.0-4.4, stirs, adjustment material temperature to 42 DEG C, insulation 12h, and period carries out stirring 10min every 30min, obtains enzymolysis slip;
(15) step (14) gained enzymolysis slip is cooled to room temperature hypsokinesis to go out, (moisture adjustment material) maize peel 120 kg and cotton dregs 105 kg is added in enzymolysis slip, the moisture content of material is adjusted to 55.17%, make solid medium, then by substratum autoclave sterilizer at hot pressing 0.7kg/cm 2, sterilizing 35 minutes under temperature 115 DEG C of conditions;
(16) by the solid culture base-material after step (15) sterilizing, when being cooled to below 45 DEG C, add by cereuisiae fermentum 43.5 kilograms, Bacillus licheniformis 21.75 kilograms and plant lactobacillus 21.75 kilograms of composite bacteria formed, stirring and evenly mixing, load in Stainless Steel Disc, bed thickness is 4-6 centimetre, moves in proving room, be placed on fermenting frame by fermented product charging tray, regulation and control room temp is 33 DEG C, room ventilated amount is 0.8m 3/ min, solid-state ventilating fermentation cultivates 36 hours, obtains fermentation culture material;
(17) by the fermentation culture material that step (16) obtains, be placed in freeze drier, temperature for-55 DEG C, vacuum tightness be 35Pa lyophilize to material moisture 5%;
(18) by step (17) dried material, through pulverizing 100 mesh sieves, adopt dry granulation machine to be directly compressed into particle, the grain diameter be pressed into was 80-90 order, through metering packing, obtained biological protein feedstuff product.
Following steps are continued, obtained biological organic fertilizer product after above-mentioned steps (6):
(19) solid phase precipitation thing that filter residue step (6) obtained and step (7) obtain merges, and with baking oven hot-air seasoning 22 hours under 95 DEG C of conditions, obtains dry slag;
(20) after the dry slag that step (19) obtains being cooled to room temperature, pulverize with Universalpulverizer, cross 85-95 mesh sieve, obtain Cucumis sativus stem, root ground-slag;
(21) Cucumis sativus stem, root ground-slag 20 kilograms that step (20) obtains is got, mix with 35 kilograms, rabbit excrement powder after mummification, 35 kilograms, sheep excrement powder after mummification and saccharomycetes to make fermentation agent 10 kilograms of proportionings, by suitable quantity of water adjustment water content to 55-60%w/w, make and wait the solid culture medium that ferments;
(22) what step (21) modulated waits the solid culture medium that ferments, load in wooden (-packing) case, bed thickness is 25 centimetres, move in the proving room of controlled epidemic disaster, first temperature be 34 DEG C, humidity be 80% condition under spontaneous fermentation 24 hours, and then adjust that room temp is 90 DEG C, humidity is 90% placement 1 week, obtain fermentation materials;
(23) by the fermentation materials that step (22) obtains, under 85 DEG C of conditions, carry out drying with rotary dryer, make drying materials be less than 5.5% to water ratio;
(24) after the siccative that step (23) obtains being cooled to room temperature, pulverize with Universalpulverizer, cross 80 mesh sieves, obtain fermentation materials powder;
(25) after the composite microbial bacteria proportioning that 94 kilograms, fermentation materials powder step (24) obtained and lactobacillus delbruckii 2 kilograms, bacillus firmus 2 kilograms and enzymatic microorganism 2 kilograms form mixes, carry out preparing burden, namely granulation, screening, packaging obtain bio-organic fertilizer product.
Embodiment 3
A working method for Curcurbitaceae mellon plant stem and root comprehensive utilization, step is as follows:
(1) get Curcurbitaceae mellon plant watermelon stem and root pulverizer is pulverized, cross 50-60 mesh sieve, obtain meal;
(2) taking the meal 100 kilograms that step (1) obtains is placed in extractor, with the pure water of 40-50 DEG C through 0.1molL -1naOH adjustment pH is the leaching solvent 800 kilograms of 10.2-10.5, and arranging rotating speed is that 45r/min constant speed stirs, and passes into steam-heated cal(l)andria, make extractive substance temperature maintain the temperature at 40-50 DEG C of hot dipping 2h through extractor interlayer;
(3) by the material after step (2) hot dipping, adjustment rotating speed is that the constant speed of 25r/min stirs, and adjustment steam intake, is heated to 100 DEG C and boils 1.5h;
(4) material after step (3) being boiled is cooled to 40-45 DEG C; Then adopt packaging type squeezing machine to squeeze, obtain pressed liquor and filter residue;
(5) filter residue obtained after step (4) being squeezed, adds pure water through 0.1molL -1naOH adjustment pH is the leaching solvent 400 kilograms of 10.2-10.5, arranges rotating speed under 25r/min constant speed stirs, is heated to 100 DEG C and boils 1h;
(6) material after step (5) being boiled is placed and is cooled to 40-45 DEG C, squeezes, obtain pressed liquor and filter residue with packaging type squeezing machine; Filter residue is for subsequent use;
(7) pressed liquor that pressed liquor step (4) obtained and step (6) obtain merges, and uses horizontal spiral centrifuge centrifugation, centrifugal rotational speed 7000r/min, obtains supernatant liquor and solid phase precipitation thing; Solid phase precipitation thing is for subsequent use;
(8) supernatant liquor that step (7) obtains is adopted the filter of 0.42 μm of millipore filtration essence, obtain smart cleaner liquid;
(9) smart cleaner liquid 0.5molL step (8) obtained -1hydrochloric acid soln regulates pH to 2.8, places 15min, when stablizing unchanged to pH value of solution, under being placed in 2-5 DEG C of environment, leaves standstill 20h, makes bryonia alcohol acid precipitate completely;
(10) step (9) bryonia alcohol acid precipitation is used 3000r/min centrifugation, washing, with recrystallizing methanol carry out refining after, in temperature be 58 DEG C, vacuum tightness is drying to obtain colorless crystalline bryonia alcohol acid 268 grams under being 0.09MPa condition, detect through HPLC, purity is 97.6%.
Following steps are continued, obtained biological protein feedstuff product after above-mentioned steps (6):
(11) the solid phase precipitation thing that filter residue step (6) obtained and step (7) obtain merges, and insert in baking oven, under 80 DEG C of conditions, warm air drying 34 hours, obtains dry slag;
(12), after the dry slag of step (11) gained being cooled to room temperature, adopting horizontal continuously stirring to pulverize and carry out micronizing, cross 350 mesh sieves after pulverizing, obtain watermelon stem, root ground-slag;
(13) take the obtained watermelon stem of step (12), root ground-slag 100 kilograms inserts in cultivation and fermentation tank the pure water 350 kilograms adding 40-45 DEG C, soak 2h, composite slip;
(14) in step (13) gains slip, add the cellulase 2.25 kilograms that unit of activity is 2000U/g, adjustment pH is 4.0-4.4, stirs, adjustment material temperature to 42 DEG C, insulation 12h, and period carries out stirring 10min every 30min, obtains enzymolysis slip;
(15) step (14) gained enzymolysis slip is cooled to room temperature hypsokinesis to go out, brewer's grains 60 kilograms and cotton dregs 50 kilograms are added in enzymolysis slip, the moisture content of material is adjusted to 62.5%, makes solid medium, then by substratum autoclave sterilizer at hot pressing 0.7kg/cm 2, sterilizing 35 minutes under temperature 115 DEG C of conditions;
(16) by the solid culture base-material after step (15) sterilizing, when being cooled to below 45 DEG C, add by fodder yeast 25.2 kilograms, subtilis 6.3 kilograms, Bacillus licheniformis 6.3 kilograms, thermophilus streptococcus 6.3 kilograms and lactobacillus bulgaricus 6.3 kilograms of composite bacteria formed, stirring and evenly mixing, load in Stainless Steel Disc, bed thickness is 4-6 centimetre, moves in proving room, be placed on fermenting frame by fermented product charging tray, regulation and control room temp is 29 DEG C, room ventilated amount is 0.8m 3/ min, solid-state ventilating fermentation cultivates 42 hours, obtains fermentation culture material;
(17) by the fermentation culture material that step (16) obtains, be placed in Vacuumdrier, temperature be 37 DEG C, vacuum tightness for vacuum-drying under-0.09MPa condition to water content 5%;
(18) by step (17) dried material, through pulverizing 100 mesh sieves, adopt dry granulation machine to be directly compressed into particle, the grain diameter be pressed into was 80-90 order, through metering packing, obtained biological protein feedstuff product.
Following steps are continued, obtained biological organic fertilizer product after above-mentioned steps (6):
(19) solid phase precipitation thing that filter residue step (6) obtained and step (7) obtain merges, and with baking oven hot-air seasoning 23 hours under 90 DEG C of conditions, obtains dry slag;
(20) after the dry slag that step (19) obtains being cooled to room temperature, pulverize with Universalpulverizer, cross 85-95 mesh sieve, obtain watermelon stem, root ground-slag;
(21) watermelon stem, root ground-slag 30 kilograms that step (20) obtains is got, 30 kilograms, rabbit excrement after mummification, cow dung after mummification 30 kilograms and saccharomycetes to make fermentation agent 10 kilograms of proportionings mix, and by suitable quantity of water adjustment water content to 55-60%w/w, make and wait the solid culture medium that ferments;
(22) what step (21) modulated waits the solid culture medium that ferments, load in wooden (-packing) case, bed thickness is 20 centimetres, move in the proving room of controlled epidemic disaster, first temperature be 33 DEG C, humidity be 70% condition under spontaneous fermentation 28 hours, and then adjust that room temp is 80 DEG C, humidity is 90% placement 1 week, obtain fermentation materials;
(23) by the fermentation materials that step (22) obtains, under 85 DEG C of conditions, carry out drying with rotary dryer, make drying materials to water content 5%;
(24) after the siccative that step (23) obtains being cooled to room temperature, pulverize with Universalpulverizer, cross 70 mesh sieves, obtain fermentation materials powder;
(25), after the composite microbial bacteria proportioning that 92.2 kilograms, fermentation materials powder step (24) obtained and Lactobacillus rogosae 1.3 kilograms, streptococcus acidi lactici 1.3 kilograms, Bacillus circulans 1.3 kilograms, subtilis 1.3 kilograms and enzymatic microorganism 2.6 kilograms form mixes, method carries out preparing burden according to the rules, namely granulation, screening, packaging obtain bio-organic fertilizer product.
Embodiment 4
A working method for Curcurbitaceae mellon plant stem and root comprehensive utilization, step is as follows:
(1) get Curcurbitaceae mellon plant pumpkin stem and root pulverizer is pulverized, cross 50-60 mesh sieve, obtain meal;
(2) taking the meal 100 kilograms that step (1) obtains is placed in extractor, with the pure water of 40-50 DEG C through 0.1molL -1naOH adjustment pH is the leaching solvent 700 kilograms of 10.2-10.5, and arranging rotating speed is that 45r/min constant speed stirs, and passes into steam-heated cal(l)andria, make extractive substance temperature maintain the temperature at 50 DEG C of hot dipping 1.5h through extractor interlayer;
(3) by the material after step (2) hot dipping, adjustment rotating speed is that the constant speed of 25r/min stirs, and adjustment steam intake, is heated to 100 DEG C and boils 2h;
(4) material after step (3) being boiled is cooled to 40-45 DEG C; Then adopt packaging type squeezing machine to squeeze, obtain squeezing juice and filter residue;
(5) filter residue obtained after step (4) being squeezed, adds pure water through 0.1molL -1naOH adjustment pH is the leaching solvent 400 kilograms of 10.2-10.5, arranges rotating speed under 25r/min constant speed stirs, is heated to 100 DEG C and boils 1.5h;
(6) material after step (5) being boiled is placed and is cooled to 40-45 DEG C, squeezes, obtain squeezing juice and filter residue with packaging type squeezing machine; Filter residue is for subsequent use;
(7) squeezing juice that squeezing juice step (4) obtained and step (6) obtain merges, and uses horizontal spiral centrifuge centrifugation, centrifugal rotational speed 7000r/min, obtains supernatant liquor and solid phase precipitation thing; Solid phase precipitation thing is for subsequent use;
(8) supernatant liquor that step (7) obtains is adopted the filter of 0.42 μm of millipore filtration essence, obtain smart cleaner liquid;
(9) smart cleaner liquid 0.5molL step (8) obtained -1hydrochloric acid soln regulates pH to 2.6, places 15min, when stablizing unchanged to pH value of solution, under being placed in 2-5 DEG C of environment, leaves standstill 20h, makes bryonia alcohol acid precipitate completely;
(10) step (9) bryonia alcohol acid precipitation is used 3500r/min centrifugation, washing, with recrystallizing methanol carry out refining after, in temperature be 60 DEG C, vacuum tightness is drying to obtain colorless crystalline bryonia alcohol acid 635.7 grams under-0.085MPa condition, detect through HPLC, purity is 95.6%.
Following steps are continued, obtained biological protein feedstuff product after above-mentioned steps (6):
(11) the solid phase precipitation thing that filter residue step (6) obtained and step (7) obtain merges, and insert in baking oven, under 75 DEG C of conditions, warm air drying 36 hours, obtains dry slag;
(12), after the dry slag of step (11) gained being cooled to room temperature, adopting horizontal continuously stirring to pulverize and carry out micronizing, cross 300 mesh sieves after pulverizing, obtain pumpkin stem, root powder;
(13) take the obtained pumpkin stem of step (12), 100 kilograms, root powder inserts in cultivation and fermentation tank the pure water 400 kilograms adding 40-45 DEG C, soak 1.5h, composite slip;
(14) in step (13) gains slip, add the cellulase 3 kilograms that unit of activity is 2000U/g, adjustment pH is 4.0-4.4, stirs, adjustment material temperature to 45 DEG C, insulation 8h, and period carries out stirring 10min every 30min, obtains enzymolysis slip;
(15) step (14) gained enzymolysis slip is cooled to room temperature hypsokinesis to go out, (moisture adjustment material) maize peel 100 kilograms, cotton dregs 20 kilograms and the dish dregs of rice 20 kilograms are added in enzymolysis slip, the moisture content of material is adjusted to 62.5%, make solid medium, then by substratum autoclave sterilizer at hot pressing 0.7kg/cm 2, sterilizing 35 minutes under temperature 115 DEG C of conditions;
(16) by the solid culture base-material after step (15) sterilizing, when being cooled to below 45 DEG C, add the composite bacteria be made up of 28.8 kg diet yeast, 7.2 kilograms of bacillus firmus, 7.2 kilograms of Bacillus licheniformis, 7.2 kilograms of plant lactobacilluss and 7.2 kilograms of Lactococcus lactis, stirring and evenly mixing, load in Stainless Steel Disc, bed thickness is 4-6 centimetre, moves in proving room, be placed on fermenting frame by fermented product charging tray, regulation and control room temp is 31 DEG C, room ventilated amount is 0.8m 3/ min, solid-state ventilating fermentation cultivates 40 hours, obtains fermentation culture material;
(17) by the fermentation culture material that step (16) obtains, be placed in Vacuumdrier, under temperature 35 DEG C, vacuum tightness-0.095MPa condition vacuum-drying to material moisture 5%;
(18) by step (17) dried material, through pulverizing 100 mesh sieves, adopt dry granulation machine to be directly compressed into particle, the grain diameter be pressed into was 80-90 order, through metering packing, obtained biological protein feedstuff product.
Following steps are continued, obtained biological organic fertilizer product after above-mentioned steps (6):
(19) solid phase precipitation thing that filter residue step (6) obtained and step (7) obtain merges, and with baking oven hot-air seasoning 25 hours under 80 DEG C of conditions, obtains dry slag;
(20) after the dry slag that step (19) obtains being cooled to room temperature, pulverize with Universalpulverizer, cross 85-95 mesh sieve, obtain pumpkin stem, root ground-slag;
(21) pumpkin stem, root ground-slag 20 kilograms that step (20) obtains is got, with dry after 35 kilograms, rabbit excrement powder, dry after 30 kilograms, cow dung powder and saccharomycetes to make fermentation agent 15 kilograms of proportionings mix, by suitable quantity of water adjustment water content to 55-60%, make and wait the solid culture medium that ferments;
(22) what step (21) modulated waits the solid culture medium that ferments, load in wooden (-packing) case, bed thickness is 20 centimetres, move in the proving room of controlled epidemic disaster, first temperature be 33 DEG C, humidity be 75% condition under spontaneous fermentation 32 hours, and then adjust that room temp is 90 DEG C, humidity is 95% placement 1 week, obtain fermentation materials;
(23) by the fermentation materials that step (22) obtains, under 85 DEG C of conditions, carry out drying with rotary dryer, make drying materials be less than 5.5% to water ratio;
(24) after the siccative that step (23) obtains being cooled to room temperature, pulverize with Universalpulverizer, cross 70 mesh sieves, obtain fermentation materials powder;
(25) after composite microbial bacteria 4.8 kilograms of proportionings that 95.2 kilograms, fermentation materials powder step (24) obtained and thermophilus streptococcus 1.2 kilograms, Bacillus licheniformis 1.2 kilograms, bacillus lentus 1.2 kilograms and enzyme element bacterium 1.2 kilograms form mix, carry out preparing burden, namely granulation, screening, packaging obtain bio-organic fertilizer product.
Embodiment 5
A working method for Curcurbitaceae mellon plant stem and root comprehensive utilization, step is as follows:
(1) get Curcurbitaceae mellon plant wax gourd stem and root pulverizer is pulverized, cross 50-60 mesh sieve, obtain meal;
(2) taking the meal 100 kilograms that step (1) obtains is placed in extractor, with the pure water of 40-50 DEG C through 0.1molL -1naOH adjustment pH is the leaching solvent 600 kilograms of 10.2-10.5, and arranging rotating speed is that 45r/min constant speed stirs, and passes into steam-heated cal(l)andria, make extractive substance temperature maintain the temperature at 40-50 DEG C of hot dipping 2.5h through extractor interlayer;
(3) by the material after step (2) hot dipping, adjustment rotating speed is that the constant speed of 25r/min stirs, and adjustment steam intake, is heated to 100 DEG C and boils 2h;
(4) material after step (3) being boiled is cooled to 40-45 DEG C; Then adopt packaging type squeezing machine to squeeze, obtain pressed liquor and filter residue;
(5) filter residue obtained after step (4) being squeezed, adds pure water through 0.1molL -1naOH adjustment pH is the leaching solvent 450 kilograms of 10.2-10.5, arranges rotating speed under 25r/min constant speed stirs, is heated to 100 DEG C and boils 1h;
(6) material after step (5) being boiled is placed and is cooled to 40-45 DEG C, squeezes, obtain pressed liquor and filter residue with packaging type squeezing machine; Filter residue is for subsequent use;
(7) pressed liquor that pressed liquor step (4) obtained and step (6) obtain merges, and uses horizontal spiral centrifuge centrifugation, centrifugal rotational speed 8000r/min, obtains supernatant liquor and solid phase precipitation thing; Solid phase precipitation thing is for subsequent use;
(8) supernatant liquor that step (7) obtains is adopted the filter of 0.22 μm of millipore filtration essence, obtain smart cleaner liquid;
(9) smart cleaner liquid 0.5molL step (8) obtained -1hydrochloric acid soln regulates pH to 3.2, places 15min, when stablizing unchanged to pH value of solution, under being placed in 2-5 DEG C of environment, leaves standstill 14h, makes bryonia alcohol acid precipitate completely;
(10) step (9) bryonia alcohol acid precipitation is used 3500r/min centrifugation, washing, with recrystallizing methanol carry out refining after, in temperature be 55 DEG C, vacuum tightness is drying to obtain colorless crystalline bryonia alcohol acid 514 grams under-0.1MPa condition, detect through HPLC, purity is 96.3%.
Following steps are continued, obtained biological protein feedstuff product after above-mentioned steps (6):
(11) the solid phase precipitation thing that filter residue step (6) obtained and step (7) obtain merges, and insert in baking oven, under 80 DEG C of conditions, warm air drying 33 hours, obtains dry slag;
(12), after the dry slag of step (11) gained being cooled to room temperature, adopting horizontal continuously stirring to pulverize and carry out micronizing, cross 350 mesh sieves after pulverizing, obtain wax gourd stem, root powder;
(13) take the obtained wax gourd stem of step (12), 100 kilograms, root powder inserts in cultivation and fermentation tank the pure water 450 kilograms adding 40-45 DEG C, soak 1.5h, composite slip;
(14) in step (13) gains slip, add the cellulase 2.75 kilograms that unit of activity is 2000U/g, adjustment pH is 4.0-4.4, stirs, adjustment material temperature to 43 DEG C, insulation 10h, and period carries out stirring 10min every 30min, obtains enzymolysis slip;
(15) step (14) gained enzymolysis slip is cooled to room temperature hypsokinesis to go out, (moisture adjustment material) maize peel 50 kilograms, 50 kilograms, rice bran and cotton dregs 100 kilograms are added in enzymolysis slip, the moisture content of material is adjusted to 60%, make solid medium, then by substratum autoclave sterilizer at hot pressing 0.7kg/cm 2, sterilizing 35 minutes under temperature 115 DEG C of conditions;
(16) by the solid culture base-material after step (15) sterilizing, when being cooled to below 45 DEG C, add by cereuisiae fermentum 30 kilograms, Bacillus licheniformis 15 kilograms, subtilis 15 kilograms, plant lactobacillus 15 kilograms and thermophilus streptococcus 15 kilograms of composite bacteria formed, stirring and evenly mixing, load in Stainless Steel Disc, bed thickness is 4-6 centimetre, moves in proving room, be placed on fermenting frame by fermented product charging tray, regulation and control room temp is 30 DEG C, room ventilated amount is 0.8m 3/ min, solid-state ventilating fermentation cultivates 40 hours, obtains fermentation culture material;
(17) by the fermentation culture material that step (16) obtains, be placed in freeze drier, temperature be-50 DEG C, vacuum tightness is that 30Pa lyophilize is less than 5% to material is moisture;
(18) by step (17) dried material, through pulverizing 100 mesh sieves, adopt dry granulation machine to be directly compressed into particle, the grain diameter be pressed into was 80-90 order, through metering packing, obtained biological protein feedstuff product.
Following steps are continued, obtained biological organic fertilizer product after above-mentioned steps (6):
(19) solid phase precipitation thing that filter residue step (6) obtained and step (7) obtain merges, and with baking oven hot-air seasoning 23 hours under 85 DEG C of conditions, obtains dry slag;
(20) after the dry slag that step (19) obtains being cooled to room temperature, pulverize with Universalpulverizer, cross 85-95 mesh sieve, obtain wax gourd stem, root ground-slag;
(21) wax gourd stem, root ground-slag 35 kilograms that step (20) obtains is got, mix with 33 kilograms, rabbit excrement powder after mummification, 20 kilograms, sheep excrement powder after mummification and saccharomycetes to make fermentation agent 12 kilograms of proportionings, by suitable quantity of water adjustment water content to 55-60%w/w, make and wait the solid culture medium that ferments;
(22) what step (21) modulated waits the solid culture medium that ferments, load in wooden (-packing) case, bed thickness is 20 centimetres, move in the proving room of controlled epidemic disaster, first temperature be 30 DEG C, humidity be 70% condition under spontaneous fermentation 30 hours, and then adjust that room temp is 80 DEG C, humidity is 85% placement 1 week, obtain fermentation materials;
(23) by the fermentation materials that step (22) obtains, under 80 DEG C of conditions, carry out drying with rotary dryer, make drying materials be less than 6% to water ratio;
(24) after the siccative that step (23) obtains being cooled to room temperature, pulverize with Universalpulverizer, cross 75 mesh sieves, obtain fermentation materials powder;
(25) after the composite microbial bacteria proportioning that 94 kilograms, fermentation materials powder step (24) obtained and streptococcus acidi lactici 1 kilogram, lactobacillus delbruckii 1 kilogram, Bacillus circulans 1 kilogram, bacillus firmus 1 kilogram and enzymatic microorganism 2 kilograms form mixes, carry out preparing burden, namely granulation, screening, packaging obtain bio-organic fertilizer product.
Embodiment 6
A working method for Curcurbitaceae mellon plant stem and root comprehensive utilization, step is as follows:
(1) get Curcurbitaceae mellon plant Muskmelon stem and root pulverizer is pulverized, cross 50 mesh sieves, obtain meal;
(2) taking the meal 100 kilograms that step (1) obtains is placed in extractor, with the pure water of 40-50 DEG C through 0.1molL -1naOH adjustment pH is the leaching solvent 800 kilograms of 10.2-10.5, and arranging rotating speed is that 45r/min constant speed stirs, and passes into steam-heated cal(l)andria, make extractive substance temperature maintain the temperature at 40 DEG C of hot dipping 2.5h through extractor interlayer;
(3) by the material after step (2) hot dipping, adjustment rotating speed is that the constant speed of 25r/min stirs, and adjustment steam intake, is heated to 100 DEG C and boils 1.5h;
(4) material after step (3) being boiled is cooled to 40-45 DEG C; Then adopt packaging type squeezing machine to squeeze, obtain pressed liquor and filter residue;
(5) filter residue obtained after step (4) being squeezed, adds pure water through 0.1molL -1naOH adjustment pH is the leaching solvent 400 kilograms of 10.2-10.5, arranges rotating speed under 25r/min constant speed stirs, is heated to 100 DEG C and boils 1.5h;
(6) material after step (5) being boiled is placed and is cooled to 40-45 DEG C, squeezes, obtain pressed liquor and filter residue with packaging type squeezing machine; Filter residue is for subsequent use;
(7) pressed liquor that pressed liquor step (4) obtained and step (6) obtain merges, and uses horizontal spiral centrifuge centrifugation, centrifugal rotational speed 7500r/min, obtains supernatant liquor and solid phase precipitation thing; Solid phase precipitation thing is for subsequent use;
(8) supernatant liquor that step (7) obtains is adopted the filter of 0.42 μm of millipore filtration essence, obtain smart cleaner liquid;
(9) smart cleaner liquid 0.5molL step (8) obtained -1hydrochloric acid soln regulates pH to 3.0, places 15min, when stablizing unchanged to pH value of solution, under being placed in 2-5 DEG C of environment, leaves standstill 20h, makes bryonia alcohol acid precipitate completely;
(10) step (9) bryonia alcohol acid precipitation is used 3000r/min centrifugation, washing, with recrystallizing methanol carry out refining after, in temperature be 55 DEG C, vacuum tightness is drying to obtain colorless crystalline bryonia alcohol acid 683.7 grams under-0.1MPa condition, detect through HPLC, purity is 97.6%.
Following steps are continued, obtained biological protein feedstuff product after above-mentioned steps (6):
(11) the solid phase precipitation thing that filter residue step (6) obtained and step (7) obtain merges, and insert in baking oven, under 80 DEG C of conditions, warm air drying 34 hours, obtains dry slag;
(12), after the dry slag of step (11) gained being cooled to room temperature, adopting horizontal continuously stirring to pulverize and carry out micronizing, cross 250 mesh sieves after pulverizing, obtain Muskmelon stem, root ground-slag;
(13) take the obtained Muskmelon stem of step (12), root ground-slag 100 kilograms inserts in cultivation and fermentation tank the pure water 400 kilograms adding 40-45 DEG C, soak 1.5h, composite slip;
(14) in step (13) gains slip, add the cellulase 2.8 kilograms that unit of activity is 2000U/g, adjustment pH is 4.0-4.4, stirs, adjustment material temperature to 45 DEG C, insulation 8h, and period carries out stirring 10min every 30min, obtains enzymolysis slip;
(15) step (14) gained enzymolysis slip is cooled to room temperature hypsokinesis to go out, maize peel 30 kilograms, brewer's grains 30 kilograms and the dish dregs of rice 80 kilograms are added in enzymolysis slip, the moisture content of material is adjusted to 62.5%, make solid medium, then by substratum autoclave sterilizer at hot pressing 0.7kg/cm 2, sterilizing 35 minutes under temperature 115 DEG C of conditions;
(16) by the solid culture base-material after step (15) sterilizing, when being cooled to below 45 DEG C, add by fodder yeast 35.2 kilograms, Bacillus coagulans 8.8 kilograms, bacstearothermophilus 8.8 kilograms, thermophilus streptococcus 8.8 kilograms and thermophilic lacto-bacilli 8.8 kilograms of composite bacteria formed, stirring and evenly mixing, load in Stainless Steel Disc, bed thickness is 4-6 centimetre, moves in proving room, be placed on fermenting frame by fermented product charging tray, regulation and control room temp is 33 DEG C, room ventilated amount is 0.8m 3/ min, solid-state ventilating fermentation cultivates 36 hours, obtains fermentation culture material;
(17) by the fermentation culture material that step (16) obtains, be placed in Vacuumdrier, temperature be 36 DEG C, vacuum tightness is less than 5% for vacuum-drying to water content under-0.095MPa condition;
(18) by step (17) dried material, through pulverizing 100 mesh sieves, adopt dry granulation machine to be directly compressed into particle, the grain diameter be pressed into was 80-90 order, through metering packing, obtained biological protein feedstuff product.
Following steps are continued, obtained biological organic fertilizer product after above-mentioned steps (6):
(19) solid phase precipitation thing that filter residue step (6) obtained and step (7) obtain merges, and with baking oven hot-air seasoning 22 hours under 95 DEG C of conditions, obtains dry slag;
(20) after the dry slag that step (19) obtains being cooled to room temperature, pulverize with Universalpulverizer, cross 85 mesh sieves, obtain Muskmelon stem, root ground-slag;
(21) Muskmelon stem, root ground-slag 25 kilograms that step (20) obtains is got, 32 kilograms, rabbit excrement after mummification, 30 kilograms, sheep excrement after mummification and saccharomycetes to make fermentation agent 13 kilograms of proportionings mix, and by suitable quantity of water adjustment water content to 55-60%w/w, make and wait the solid culture medium that ferments;
(22) what step (21) modulated waits the solid culture medium that ferments, load in wooden (-packing) case, bed thickness is 25 centimetres, move in the proving room of controlled epidemic disaster, first temperature be 32 DEG C, humidity be 75% condition under spontaneous fermentation 32 hours, and then adjust that room temp is 85 DEG C, humidity is 90% placement 1 week, obtain fermentation materials;
(23) by the fermentation materials that step (22) obtains, under 90 DEG C of conditions, carry out drying with rotary dryer, make drying materials be less than 6% to water content;
(24) after the siccative that step (23) obtains being cooled to room temperature, pulverize with Universalpulverizer, cross 65 mesh sieves, obtain fermentation materials powder;
(25), after the composite microbial bacteria proportioning that 92.5 kilograms, fermentation materials powder step (24) obtained and lactobacillus delbruckii 1.25 kilograms, thermophilus streptococcus 1.25 kilograms, bacillus firmus 1.25 kilograms, Bacillus licheniformis 1.25 kilograms and enzymatic microorganism 2.5 kilograms form mixes, method carries out preparing burden according to the rules, namely granulation, screening, packaging obtain bio-organic fertilizer product.
Embodiment 7
A working method for Curcurbitaceae mellon plant stem and root comprehensive utilization, step is as follows:
(1) get Curcurbitaceae mellon plant snake melon stem and root pulverizer is pulverized, cross 55 mesh sieves, obtain meal;
(2) taking the meal 100 kilograms that step (1) obtains is placed in extractor, with the pure water of 40-50 DEG C through 0.1molL -1naOH adjustment pH is the leaching solvent 750 kilograms of 10.2-10.5, and arranging rotating speed is that 45r/min constant speed stirs, and passes into steam-heated cal(l)andria, make extractive substance temperature maintain the temperature at 50 DEG C of hot dipping 1.5h through extractor interlayer;
(3) by the material after step (2) hot dipping, adjustment rotating speed is that the constant speed of 25r/min stirs, and adjustment steam intake, is heated to 100 DEG C and boils 3h;
(4) material after step (3) being boiled is cooled to 40-45 DEG C; Then adopt packaging type squeezing machine to squeeze, obtain pressed liquor and filter residue;
(5) filter residue obtained after step (4) being squeezed, adds pure water through 0.1molL -1naOH adjustment pH is the leaching solvent 450 kilograms of 10.2-10.5, arranges rotating speed under 25r/min constant speed stirs, is heated to 100 DEG C and boils 1.5h;
(6) material after step (5) being boiled is placed and is cooled to 40-45 DEG C, squeezes, obtain pressed liquor and filter residue with packaging type squeezing machine; Filter residue is for subsequent use;
(7) pressed liquor that pressed liquor step (4) obtained and step (6) obtain merges, and uses horizontal spiral centrifuge centrifugation, centrifugal rotational speed 6500r/min, obtains supernatant liquor and solid phase precipitation thing; Solid phase precipitation thing is for subsequent use;
(8) supernatant liquor that step (7) obtains is adopted the filter of 0.42 μm of millipore filtration essence, obtain smart cleaner liquid;
(9) smart cleaner liquid 0.5molL step (8) obtained -1hydrochloric acid soln regulates pH to 2.6, places 15min, when stablizing unchanged to pH value of solution, under being placed in 2-5 DEG C of environment, leaves standstill 20h, makes bryonia alcohol acid precipitate completely;
(10) step (9) bryonia alcohol acid precipitation is used 3500r/min centrifugation, washing, with recrystallizing methanol carry out refining after, in temperature be 55 DEG C, vacuum tightness is drying to obtain colorless crystalline bryonia alcohol acid 567 grams under-0.1MPa condition, detect through HPLC, purity is 97.6%.
Following steps are continued, obtained biological protein feedstuff product after above-mentioned steps (6):
(11) the solid phase precipitation thing that filter residue step (6) obtained and step (7) obtain merges, and insert in baking oven, under 85 DEG C of conditions, warm air drying 30 hours, obtains dry slag;
(12), after the dry slag of step (11) gained being cooled to room temperature, adopting horizontal continuously stirring to pulverize and carry out micronizing, cross 300 mesh sieves after pulverizing, obtain snake melon stem, root ground-slag;
(13) take the obtained snake melon stem of step (12), root ground-slag 100 kilograms inserts in cultivation and fermentation tank the pure water 480 kilograms adding 40-45 DEG C, soak 2h, composite slip;
(14) in step (13) gains slip, add the cellulase 2.7 kilograms that unit of activity is 2000U/g, adjustment pH is 4.0-4.4, stirs, adjustment material temperature to 43 DEG C, insulation 10h, and period carries out stirring 10min every 30min, obtains enzymolysis slip;
(15) step (14) gained enzymolysis slip is cooled to room temperature hypsokinesis to go out, wheat bran 120 kg and the dish dregs of rice 100 kilograms are added in enzymolysis slip, the moisture content of material is adjusted to 60%, makes solid medium, then by substratum autoclave sterilizer at hot pressing 0.7kg/cm 2, sterilizing 35 minutes under temperature 115 DEG C of conditions;
(16) by the solid culture base-material after step (15) sterilizing, when being cooled to below 45 DEG C, add by fodder yeast 35.2 kilograms, subtilis 8.8 kilograms, Bacillus coagulans 8.8 kilograms, thermophilic lacto-bacilli 8.8 kilograms and plant lactobacillus 8.8 kilograms of composite bacteria formed, stirring and evenly mixing, load in Stainless Steel Disc, bed thickness is 4-6 centimetre, moves in proving room, be placed on fermenting frame by fermented product charging tray, regulation and control room temp is 32 DEG C, room ventilated amount is 0.8m 3/ min, solid-state ventilating fermentation cultivates 38 hours, obtains fermentation culture material;
(17) by the fermentation culture material that step (16) obtains, be placed in Vacuumdrier, temperature be 36 DEG C, vacuum tightness for vacuum-drying under-0.095MPa condition to water content 5%;
(18) by step (17) dried material, through pulverizing 100 mesh sieves, adopt dry granulation machine to be directly compressed into particle, the grain diameter be pressed into was 80-90 order, through metering packing, obtained biological protein feedstuff product.
Following steps are continued, obtained biological organic fertilizer product after above-mentioned steps (6):
(19) solid phase precipitation thing that filter residue step (6) obtained and step (7) obtain merges, and with baking oven hot-air seasoning 23 hours under 90 DEG C of conditions, obtains dry slag;
(20) after the dry slag that step (19) obtains being cooled to room temperature, pulverize with Universalpulverizer, cross 95 mesh sieves, obtain snake melon stem, root ground-slag;
(21) get snake melon stem, root ground-slag 35 kilograms that step (20) obtains, 53 kilograms, rabbit excrement powder after mummification, saccharomycetes to make fermentation agent 12 kilograms of proportionings mix, and by suitable quantity of water adjustment water content to 55-60%w/w, make and wait the solid culture medium that ferments;
(22) what step (21) modulated waits the solid culture medium that ferments, load in wooden (-packing) case, bed thickness is 20 centimetres, move in the proving room of controlled epidemic disaster, first temperature be 30 DEG C, humidity be 65% condition under spontaneous fermentation 33 hours, and then adjust that room temp is 75 DEG C, humidity is 85% placement 1 week, obtain fermentation materials;
(23) by the fermentation materials that step (22) obtains, under 90 DEG C of conditions, carry out drying with rotary dryer, make drying materials to water content 5%;
(24) after the siccative that step (23) obtains being cooled to room temperature, pulverize with Universalpulverizer, cross 80 mesh sieves, obtain fermentation materials powder;
(25), after the composite microbial bacteria proportioning that 93.4 kilograms, fermentation materials powder step (24) obtained and lactobacillus delbruckii 1.1 kilograms, Lactobacillus rogosae 1.1 kilograms, Bacillus licheniformis 1.1 kilograms, subtilis 1.1 kilograms and enzymatic microorganism 2.2 kilograms form mixes, method carries out preparing burden according to the rules, namely granulation, screening, packaging obtain bio-organic fertilizer product.

Claims (41)

1. the working method of Curcurbitaceae mellon plant stem and root comprehensive utilization, is characterized in that following steps obtain bryonia alcohol acid product:
(1) get Curcurbitaceae mellon plant stem and root, pulverize with pulverizer, cross 50-60 mesh sieve, obtain meal;
(2) in step (1) meal, 40-50 DEG C is added with pure water through 0.1molL -1naOH regulates pH to be the leaching solvent of 10.2-12, and the weight ratio of meal and solvent is 1:(4-15), rotating speed is set under the constant speed, uniform stirring of 45r/min, is incubated 40-50 DEG C of hot dipping 1-4h;
(3) by the material after step (2) hot dipping, then rotating speed is adjusted under the constant speed, uniform stirring of 25r/min, heated and boiled 1.5-3h;
(4) material after step (3) being boiled is placed and is cooled to 40-45 DEG C; Then squeeze, obtain pressed liquor and filter residue;
(5) step (4) filter residue is added with pure water through 0.1molL -1naOH regulates pH to be the leaching solvent of 10.2-12, and the amount adding solvent is meal: the weight ratio of solvent is 1:3-10; Rotating speed is set under the constant speed, uniform stirring of 25r/min, heated and boiled 1-2h;
(6) material after step (5) being boiled is placed and is cooled to 40-45 DEG C, then squeezes, obtains pressed liquor and filter residue; Filter residue is for subsequent use;
(7) pressing filtering liquid step (4) and step (6) obtained carries out centrifugation, centrifugal rotational speed 5000-10000r/min, obtains supernatant liquor and solid phase precipitation thing after merging; Solid phase precipitation thing is for subsequent use;
(8) supernatant liquor that step (7) obtains is carried out essence filter, obtain smart cleaner liquid;
(9) smart cleaner liquid 0.5molL step (8) obtained -1hydrochloric acid soln regulates pH to 2.5-3.2, places 15min, when stablizing unchanged to pH value of solution, under putting 2-5 DEG C of environment, leaves standstill 20h, makes bryonia alcohol acid precipitate completely;
(10) step (9) bryonia alcohol acid is precipitated through 3000-3500r/min centrifugation, washing, refine with ordinary method recrystallization, vacuum-drying, obtain bryonia alcohol acid product.
2. the working method of Curcurbitaceae mellon plant stem as claimed in claim 1 and root comprehensive utilization, is characterized in that, after above-mentioned steps (6), continue following steps, obtained biological protein feedstuff product:
(11) the solid phase precipitation thing that filter residue step (6) obtained and step (7) obtain merges, and under 60 DEG C of-85 DEG C of conditions, dry 24-36 hour, obtains dry slag;
(12) the dry slag of step (11) gained is cooled to room temperature, pulverizing, the dry slag after pulverizing crosses 250-350 mesh sieve, obtains mellon plant stem root ground-slag; Meal returns to be pulverized again;
(13) pure water adding 40-45 DEG C inserted in cultivation and fermentation tank by mellon plant stem root ground-slag step (12) obtained, and the weight ratio of mellon plant stem, root ground-slag and pure water is 1:(2-10), soak 1-2h, composite slip;
(14) in step (13) gains slip, add the cellulase that unit of activity is 2000U/g weight 0.3-0.6%, adjustment pH is 4-5.5, and be heated to 40-50 DEG C, insulation 5-14h, period carries out stirring 10min every 30min, obtains enzymolysis slip;
(15) step (14) gained enzymolysis slip is cooled to room temperature to release, in enzymolysis slip, adds moisture adjustment material, the moisture of whole material is adjusted to 55-65%w/w, makes solid medium, then culture base-material is carried out sterilizing;
(16) by the solid culture base-material after step (15) sterilizing through naturally cooling to below 45 DEG C time, add culture base-material weight 8-12% composite bacteria, mixing material, load in Stainless Steel Disc, bed thickness is 4-6 centimetre, in immigration proving room, be placed on fermenting frame by fermented product charging tray, regulation and control room temp is 25-36 DEG C, room ventilated amount is 0.8m 3under/min condition, solid-state ventilating fermentation cultivates 30-50 hour, obtains fermentation culture material;
(17) by the fermentation culture material that step (16) obtains, low-temperature vacuum drying or lyophilize is adopted;
(18) by step (17) dried material, through pulverizing 100 mesh sieves, adopt dry granulation machine to be directly compressed into particle, the grain diameter be pressed into was 80-90 order, through metering, packaging, obtained biological protein feedstuff product.
3. the working method of Curcurbitaceae mellon plant stem as claimed in claim 1 and root comprehensive utilization, is characterized in that, after above-mentioned steps (6), continue following steps, obtained biological organic fertilizer product:
(19) the solid phase precipitation thing that filter residue step (6) obtained and step (7) obtain merges, and dries 20-32 hour, obtain dry slag under 60-95 DEG C of condition;
(20) the dry slag that step (19) obtains is cooled to room temperature to pulverize, crosses 85-95 mesh sieve, obtain mellon plant stem root ground-slag;
(21) mellon plant stem root ground-slag step (20) obtained mixes with animal excreta and saccharomycetes to make fermentation agent, and the adjustment water content that adds water, to 55-60%w/w, is modulated into and treats fermentation solid medium material; Wherein animal excreta is the animal excreta after drying and other treatment; Wherein the weight percent of mellon plant stem root ground-slag, animal excreta and saccharomycetes to make fermentation agent is: mellon plant stem root ground-slag 20-40%, animal excreta 50-70%, saccharomycetes to make fermentation agent 10-15% ratio;
(22) what step (21) modulated treats fermentation solid medium material, load in wooden or bamboo case, bed thickness is 20-25 centimetre, move in the wet proving room of controlled temperature control, first temperature be 29-34 DEG C, humidity be 60-80% condition under spontaneous fermentation 24-36 hour, then adjust room temp be 75-90 DEG C, humidity be under 85-95% condition place 1 week, obtain fermentation materials;
(23) by the fermentation materials that step (22) obtains, under 60-90 DEG C of condition, carry out drying, make drying materials to water ratio 6% or lower;
(24) after the siccative that step (23) obtains being cooled to room temperature, pulverize, cross 60-80 mesh sieve, obtain fermentation materials powder;
(25) fermentation material powder step (24) obtained and composite microbial bacteria are fermentation material powder 92.2-95.2% in mass ratio, composite microbial bacteria 4.8-7.8% proportions, after mixing, method is routinely carried out preparing burden, granulation, screening, is namely obtained bio-organic fertilizer product after packaging.
4. the working method of the Curcurbitaceae mellon plant stem as described in claim 1 or 2 or 3 and root comprehensive utilization, it is characterized in that, the Curcurbitaceae mellon plant described in described step (1) is sponge gourd (Luffa cylindrica), cucumber (Cucumis sativus), watermelon (Citrullus lanatus), pumpkin (Cucurbita moschata), wax gourd (Benincasa cerifera), muskmelon (Cucumis melo) and snake melon (Cucumis melo var.conomon).
5. the working method of Curcurbitaceae mellon plant stem as claimed in claim 4 and root comprehensive utilization, it is characterized in that, described Curcurbitaceae mellon plant is dry product or fresh goods.
6. the working method of Curcurbitaceae mellon plant stem as claimed in claim 5 and root comprehensive utilization, it is characterized in that, described Curcurbitaceae mellon plant is dry product.
7. the working method of the Curcurbitaceae mellon plant stem as described in claim 1 or 2 or 3 and root comprehensive utilization, is characterized in that, in described step (2), the weight ratio of meal and solvent is preferably 1:(5-10); Insulation 40-50 DEG C, hot dipping time preferred 1.5-2.5h in step (2).
8. the working method of the Curcurbitaceae mellon plant stem as described in claim 1 or 2 or 3 and root comprehensive utilization, it is characterized in that, the preferred pH of leaching solvent described in described step (2) is 10.2-10.5.
9. the working method of the Curcurbitaceae mellon plant stem as described in claim 1 or 2 or 3 and root comprehensive utilization, it is characterized in that, the heated and boiled time in described step (3) is preferably 1.5-2h; Packaging type squeezing machine is selected in squeezing described in described step (4).
10. the working method of the Curcurbitaceae mellon plant stem as described in claim 1 or 2 or 3 and root comprehensive utilization, it is characterized in that, in described step (5), preferred pH scope is 10.2-10.5; Preferred meal: the weight ratio of solvent is 1:(3-5); Packaging type squeezing machine is selected in squeezing described in described step (6).
The working method of 11. Curcurbitaceae mellon plant stems as described in claim 1 or 2 or 3 and root comprehensive utilization, it is characterized in that, the pressed liquor centrifugation that is combined described in described step (7) preferably uses horizontal spiral centrifuge; Preferred centrifugal rotational speed is 6000-8000r/min.
The working method of 12. Curcurbitaceae mellon plant stems as claimed in claim 1 or 2 and root comprehensive utilization, is characterized in that, the filtering with microporous membrane that the essence filter described in described step (8) uses aperture to be less than 0.45 μm.
The working method of 13. Curcurbitaceae mellon plant stems as claimed in claim 1 and root comprehensive utilization, it is characterized in that, the described vacuum drying condition of described step (10) is temperature 55-60 DEG C, vacuum tightness-0.085--0.1MPa.
14. the working method of Curcurbitaceae mellon plant stem as claimed in claim 2 and root comprehensive utilization, it is characterized in that, the drying described in described step (11) adopts warm air drying or far infrared drying; Preferred drying conditions be under 75-85 DEG C of condition, dry 30-36 hour.
The working method of 15. Curcurbitaceae mellon plant stems as claimed in claim 2 and root comprehensive utilization, it is characterized in that, the pulverizing described in described step (12) is micronizing; The horizontal continuously stirring pulverizer of preferred use.
The working method of 16. Curcurbitaceae mellon plant stems as claimed in claim 2 and root comprehensive utilization, is characterized in that, in described step (13), the preferred weight ratio of mellon plant stem root ground-slag and water is 1:(3-5); The preferred condition of enzymolysis described in step (14) is pH4.0-4.4, temperature 42-45 DEG C, time 8-12h.
The working method of 17. Curcurbitaceae mellon plant stems as claimed in claim 2 and root comprehensive utilization, it is characterized in that, the moisture adjustment material described in described step (15) is selected from wheat bran, rice bran, maize peel, brewer's grains, the dish dregs of rice, cotton dregs any one or its mixture.
The working method of 18. Curcurbitaceae mellon plant stems as claimed in claim 17 and root comprehensive utilization, is characterized in that, described moisture adjustment material is selected from maize peel, brewer's grains, the dish dregs of rice, cotton dregs or its mixture.
The working method of 19. Curcurbitaceae mellon plant stems as described in claim 17 or 18 and root comprehensive utilization, is characterized in that, the add-on of described moisture adjustment material makes moisture content of material be down to 56-63%W/W.
20. the working method of Curcurbitaceae mellon plant stem as claimed in claim 2 and root comprehensive utilization, is characterized in that, the sterilizing described in described step (15), adopt hot pressing 0.7Kg/cm 2, 115 DEG C, sterilizing 35 minutes.
The working method of 21. Curcurbitaceae mellon plant stems as claimed in claim 2 and root comprehensive utilization, it is characterized in that, the mixed bacterium that the composite bacteria described in described step (16) contains following bacterial classification is yeast by yeast (Saccharomyces), genus bacillus (Bacillus) and milk-acid bacteria (Lactobacillus) by weight: genus bacillus: milk-acid bacteria=2:1:1 proportions mixes.
The working method of 22. Curcurbitaceae mellon plant stems as claimed in claim 21 and root comprehensive utilization, it is characterized in that, described yeast adopts every gram commercially available to be no less than Candida utilis (Candida utilis), cereuisiae fermentum (Saccharomyces cerevisiae) and the fodder yeast (Candida Tropicali) of 20,000,000,000 containing viable bacteria concentration.
The working method of 23. Curcurbitaceae mellon plant stems as claimed in claim 22 and root comprehensive utilization, it is characterized in that, described yeast is fodder yeast (Candida Tropicali).
The working method of 24. Curcurbitaceae mellon plant stems as claimed in claim 21 and root comprehensive utilization, it is characterized in that, described genus bacillus adopts every gram commercially available to be no less than the subtilis (Bacillus subtilis) of 20,000,000,000 containing number of viable, Bacillus licheniformis (Bacillus licheniformis), Bacillus coagulans (Bacillus coagulans), one or more arbitrary combination in bacstearothermophilus (Bacillus stearothermophilus) and bacillus firmus (Bacillusfirmus) thalline dry powder goods.
The working method of 25. Curcurbitaceae mellon plant stems as claimed in claim 21 and root comprehensive utilization, it is characterized in that, described milk-acid bacteria (Lactobacillus) adopts every gram commercially available to be no less than the thermophilus streptococcus (Streptococcus thermophilus) of 20,000,000,000 containing number of viable, thermophilic lacto-bacilli (Lactobacillus thermophilus), plant lactobacillus (Lactobacillus plantarum), Lactococcus lactis (Lactococcus lactis), one or more arbitrary combination in lactobacillus bulgaricus (Lactobacillus bulgaricus) thalline dry powder goods.
The working method of 26. Curcurbitaceae mellon plant stems as claimed in claim 2 and root comprehensive utilization, is characterized in that, in the proving room described in described step (16), preferred temperature is 29-33 DEG C; Preferred fermented incubation time is 36-42 hour.
The working method of 27. Curcurbitaceae mellon plant stems as claimed in claim 2 and root comprehensive utilization, it is characterized in that, the condition of the low-temperature vacuum drying described in described step (17) is temperature 34-37 DEG C, vacuum tightness-0.09--0.1MPa.
The working method of 28. Curcurbitaceae mellon plant stems as claimed in claim 2 and root comprehensive utilization, it is characterized in that, the cryodesiccated condition described in described step (17) is temperature-55--50 DEG C, vacuum tightness 25-35Pa.
The working method of 29. Curcurbitaceae mellon plant stems as claimed in claim 3 and root comprehensive utilization, is characterized in that, the oven dry described in described step (19) adopts hot-air seasoning; Preferred drying condition for dry 22-25 hour under 80-95 DEG C of condition.
The working method of 30. Curcurbitaceae mellon plant stems as claimed in claim 3 and root comprehensive utilization, is characterized in that, the animal excreta in described step (21) is the mixture of herbivorous animal rabbit excrement, sheep excrement, cow dung, horsehit, camel excrement or its arbitrary combination.
The working method of 31. Curcurbitaceae mellon plant stems as claimed in claim 30 and root comprehensive utilization, is characterized in that, described animal excreta adopts the mixture of rabbit excrement, sheep excrement, cow dung or its arbitrary combination.
The working method of 32. Curcurbitaceae mellon plant stems as claimed in claim 3 and root comprehensive utilization, it is characterized in that, animal excreta drying and other treatment in described step (21) carries out drying to water content < 5% through 135 DEG C for adopting dryer, applies after crossing 80 mesh sieves.
The working method of 33. Curcurbitaceae mellon plant stems as claimed in claim 3 and root comprehensive utilization, is characterized in that, the drying in described step (23) adopts rotary dryer; Preferred drying conditions is temperature 80-90 DEG C.
The working method of 34. Curcurbitaceae mellon plant stems as claimed in claim 3 and root comprehensive utilization, it is characterized in that, the composite microbial bacteria described in described step (25) is mixed in proportion by the milk-acid bacteria (Lactobacillus) of following bacterial classification, genus bacillus (Bacillus) and enzymatic microorganism and forms; Preferred weight part ratio is milk-acid bacteria: genus bacillus: enzymatic microorganism=1:1:1 ratio.
The working method of 35. Curcurbitaceae mellon plant stems as claimed in claim 34 and root comprehensive utilization, it is characterized in that, described milk-acid bacteria and genus bacillus adopt commercially available every gram to be no less than milk-acid bacteria and the genus bacillus thalline dry powder goods of 20,000,000,000 containing number of viable.
The working method of 36. Curcurbitaceae mellon plant stems as claimed in claim 34 and root comprehensive utilization, is characterized in that, described enzymatic microorganism adopts commercially available every gram to be no less than the dry powder goods of 5,000 ten thousand containing number of viable.
The working method of 37. Curcurbitaceae mellon plant stems as described in claim 34 or 35 and root comprehensive utilization, it is characterized in that, described milk-acid bacteria adopts one or more arbitrary combination in thermophilus streptococcus (Streptococcus thermophilus), streptococcus acidi lactici (Streptococcus acidi lactici), Lactobacterium acidophilum (Bacillus acidophilus), lactobacillus delbruckii (Lactobacillus delbriickii), Lactobacillus rogosae (Lactobacillus rentdril).
The working method of 38. Curcurbitaceae mellon plant stems as described in claim 34 or 35 and root comprehensive utilization, it is characterized in that, described genus bacillus adopts one or more arbitrary combination in Bacillus licheniformis (Bacillus licheniform is), Bacillus circulans (Bacillus circulans Jordan), subtilis (Bacillus subtilis), bacillus firmus (Bacillusfirmus) and bacillus lentus (Bacillus lentus).
The working method of 39. 1 kinds of Curcurbitaceae mellon plant stems as claimed in claim 1 and root comprehensive utilization, obtains bryonia alcohol acid product.
The working method of 40. 1 kinds of Curcurbitaceae mellon plant stems as claimed in claim 2 and root comprehensive utilization, obtains biological protein feedstuff product.
The working method of 41. 1 kinds of Curcurbitaceae mellon plant stems as claimed in claim 3 and root comprehensive utilization, obtains bio-organic fertilizer product.
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Publication number Priority date Publication date Assignee Title
CN104957633A (en) * 2015-07-17 2015-10-07 唐玉贵 Plant enzyme solid preparation and preparation method thereof
CN105272442A (en) * 2015-09-23 2016-01-27 广西壮锦家园农业科技发展有限公司 Chinese herbal medicine bio-fertilizer and production method
CN107759296A (en) * 2016-08-16 2018-03-06 曾绍珍 A kind of cassava knot potato stage special fertilizer
CN115104531A (en) * 2022-06-21 2022-09-27 珀莱雅化妆品股份有限公司 Method for increasing bryonolic acid content in towel gourd root and extracting towel gourd root

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CN102002087A (en) * 2010-10-26 2011-04-06 南京泽朗医药科技有限公司 Method for preparing bryonia alcohol acid
CN103214544A (en) * 2013-05-17 2013-07-24 张丽 Method for extracting ursolic acid from paulownia leaves

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011029170A1 (en) * 2009-09-13 2011-03-17 Valsanic Enterprises Llc Process to obtain a homeopathic medicament and use thereof
CN102002087A (en) * 2010-10-26 2011-04-06 南京泽朗医药科技有限公司 Method for preparing bryonia alcohol acid
CN103214544A (en) * 2013-05-17 2013-07-24 张丽 Method for extracting ursolic acid from paulownia leaves

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104957633A (en) * 2015-07-17 2015-10-07 唐玉贵 Plant enzyme solid preparation and preparation method thereof
CN105272442A (en) * 2015-09-23 2016-01-27 广西壮锦家园农业科技发展有限公司 Chinese herbal medicine bio-fertilizer and production method
CN107759296A (en) * 2016-08-16 2018-03-06 曾绍珍 A kind of cassava knot potato stage special fertilizer
CN115104531A (en) * 2022-06-21 2022-09-27 珀莱雅化妆品股份有限公司 Method for increasing bryonolic acid content in towel gourd root and extracting towel gourd root
CN115104531B (en) * 2022-06-21 2023-10-13 珀莱雅化妆品股份有限公司 Method for improving content of bryophyte alkyd in luffa roots and extracting bryophyte alkyd

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