CN104496728A - Compound fertilizer for increasing flavone content of fagopyrum tataricum - Google Patents
Compound fertilizer for increasing flavone content of fagopyrum tataricum Download PDFInfo
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- CN104496728A CN104496728A CN201410852576.0A CN201410852576A CN104496728A CN 104496728 A CN104496728 A CN 104496728A CN 201410852576 A CN201410852576 A CN 201410852576A CN 104496728 A CN104496728 A CN 104496728A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
Abstract
The invention discloses a compound fertilizer for increasing the flavone content of fagopyrum tataricum. The compound fertilizer comprises the following components in parts by weight: 100-150 parts of dry cattle and sheep manure, 5-20 parts of urea, 1-10 parts of calcium superphosphate, 1-10 parts of bamboo charcoal powder, 1-5 parts of sodium borate, 1-2 parts of ferrous sulfate, 1-2 parts of zinc sulfate, 1-2 parts of sodium selenite, 0.5-2 parts of potassium dihydrogen phosphate and 0.1-0.5 part of ammonium molybdate. According to the compound fertilizer, the flavone content of a fagopyrum tataricum kernel is increased by 40%-60%, the content of microelements, namely zinc, selenium, iron and the like is greatly increased, the total yield reaches more than 160 kilograms per mu, the soil quality is improved, and the economic benefit is obviously enhanced.
Description
Technical field:
The present invention relates to a kind of chemical fertilizer, particularly relate to a kind of composite fertilizer of improving tartary wheet flavone content, belong to plantation technical field of fertilizers.
Background technology:
Buckwheat (buckwheat) belongs to polygonaceae (Polygonaceae) medicinal and edible plant, nutritious, have the laudatory title of " kings of five cereals ".Nutritive value due to its uniqueness is considered to global emerging crop.It is little ancestor crop, but the deficiency that can make up staple crop advantage and the composition do not had: it can be planted in, and the breeding time that staple crop can not plant is short, cool region and hungry soil, the nutritive ingredient that it not only contains containing staple crop, but also do not contain containing staple crop and also be human body institute essential component.
Buckwheat whole body is precious, sprout tender leaf, cauline leaf flowers and fruits, root and stalk, none refuse of shell rice and flour.Volume increase of increasing soil fertility is used from natural resources, from agricultural to aquaculture, produce from foodstuffs industry to light industry, from food (food and medicine consangunity) to health care diseases prevention, from domestic market to world market, in the tourist industry of particularly day by day rising at Aba Prefecture, there are immeasurable market outlook, in featured agriculture, buckwheat more will show distinctive economic worth.Therefore, greatly develop buckwheat and produce, the resources advantage becoming buckwheat is commodity advantage, realizes increasing both production and income, for disease of uplifting the people's living standard, defend, promotes that rural economic development and high halfway up the hill are shaken off poverty and set out on the road to prosperity, all has important practical significance.In recent years, along with deepening continuously of the aspect researchs such as agricultural, medical science and food science, the enhancing gradually of health of people consciousness, the market demand of buckwheat increases day by day, and the status in world commerce is also raised gradually.Especially in recent years, along with the raising of people's living standard, deepening continuously of buckwheat scientific research and new product development, people deepen continuously to buckwheat medical treatment and health value understanding, and the industry size of buckwheat grows stronger day by day as the development of buckwheat brings excellent opportunity.
Flavonoid compound is the main component of buckwheat polyphenols, mainly comprises: rutin, Quercetin 3-galactoside, oak skin element and Quercetin etc.Research shows, the flavonoid compound in buckwheat has different physiological roles: the function with SOD, can scavenging activated oxygen; Rutin wherein has prophylactic effect to hematencephalon, anemia; Rutin can also suppress the activity of Zinc metallopeptidase Zace1, has active effect to the control of vascular hypertension and treatment; Hypoglycemic, lipid-lowering effect is obvious; Quercetin in buckwheat can inhibition tumor cell growth, makes Carcinoma cell differentiation, suppresses chemical toxicity or the carinogenicity of xenobiotic thing by enzyme system in control agent.Because Flavone Content of Buckwheat is on the low side, add the perfect not of extraction process, seriously limit the development of buckwheat.
Summary of the invention:
The object of this invention is to provide a kind of composite fertilizer of improving tartary wheet flavone content, improve the micronutrient levels such as flavones and zinc, selenium, iron in buckwheat, improve buckwheat ultimate production, reduce disease, improvement soil quality, increases economic efficiency.
For achieving the above object, technical scheme of the present invention is:
Improve a composite fertilizer for tartary wheet flavone content, it is characterized in that:
Its component and parts by weight comprise: dry cattle and sheep excrement 100-150 part, urea 5-20 part, calcium superphosphate 1-10 part, bamboo charcoal powder 1-10 part, boric acid 1-5 part, ferrous sulfate 1-2 part, zinc sulfate 1-2 part, Sodium Selenite 1-2 part, potassium primary phosphate 0.5-2 part, ammonium molybdate 0.1-0.5 part.
Preferably, its component and parts by weight comprise: dry cattle and sheep excrement 120-150 part, urea 8-20 part, calcium superphosphate 5-10 part, bamboo charcoal powder 2-10 part, boric acid 1-5 part, ferrous sulfate 1-2 part, zinc sulfate 1-2 part, Sodium Selenite 1-2 part, potassium primary phosphate 0.5-2 part, ammonium molybdate 0.1-0.5 part.
The physiological action of boron is: participate in metabolism and the transport of making sugar in object; Suppress the biosynthesizing of phenolic compound and xylogen; Promote the growth of pollen germination and pollen tube; Promote growing of seed and fruit; Affect cell fission, differentiation, maturation; The metabolism of involved in plant auxins hormone; Promote that plant is to the picked-up of calcium.The general symptom of boron deficiency: vegetative point is downright bad; Vascular bundle is impaired, root system development is bad; Crop top branch growth is unusual; Leaf deformity; Crop growth stage fruit drop; Crop pollination insemination and fruit are formed not good enough; Deformed fruit and seeds abortion; Breeding time postpones; Impaired and the insufficient quality of crop yield.Spraying borax, facilitates buckwheat and blooms, pollinates and seed growth, thus improve the flavones content of seed.
Phosphorus can promote the growth of flower and fruit, and potassium can make well developed root system, and blade plumpness is dark green, and stem stalk is sturdy, and disease resistance, resistance strengthen, and promotes Starch formation.After foliage-spray potassium primary phosphate, buckwheat stem stalk can be made sturdy, and blade plumpness is dark green, and photosynthesis strengthens, and seed starch enriches, and then improves flavones content in buckwheat seed.
Molybdenum is the composition of nitrate reductase, the nitrogen fixing capacity of root nodule bacterium can be improved, promote the reductive action of nitrate in plant materials, absorbing namely to nitric nitrogen, inorganic phosphorus in plant materials can also be strengthened and be transformed into the ability of organophosphorus, eliminate the toxic action that active aluminum and manganese in soil are too much, molybdenum element is also the required nutritive element of plant, is the moiety of coenzyme in organism, enzyme.Lack molybdenum symptom and first show that Lao Ye occurs then on young leaves.Sometimes vegetative point is dead, and it is real not full that Floral development is suppressed son.There is provided sufficient molybdenum element can improve flavones content in buckwheat seed.
Preferably, its component also comprises subtilis culture, bacillus megaterium culture and rhizobia cultures, and parts by weight are respectively 1-3 part, 3-5 part and 2-6 part.
Preferably, the culturing step of its subtilis culture, bacillus megaterium culture and rhizobia cultures is:
The first step: the 120 DEG C of sterilizings of first liquid substratum were mixed with weight ratio 100:1 with tween after 20 minutes, subtilis, bacillus megaterium mixture are inoculated in above-mentioned substratum according to the ratio of weight ratio 1:100, abundant stirring airtight standing for fermentation 4-7 days after 10 minutes, sampling Detection thalli growth situation and measure pH value, plate count, when viable bacteria total concn is more than 10
9/ ML and pH value just stop fermentation at 3.0-4.0;
Second step: the 120 DEG C of sterilizings of second liquid substratum were ventilated after 20 minutes, root nodule bacterium are inoculated in second liquid substratum according to weight ratio 1:100 ratio, abundant stirring airtight standing for fermentation 1-4 days after 10 minutes, sampling Detection thalli growth situation and measure pH value, plate count, when viable bacteria total concn is more than 10
9namely/ML stops fermentation;
3rd step: by the first step with 35-40 DEG C of warm air drying 10-20 hour after under second step gained culture relative centrifugal force 3000-4000 × g centrifugal 10-30 minute, make water content be reduced to less than 25%;
4th step: the microbial strain culture that the 3rd step obtains mixed according to 1:100 part by weight with microbe carrier, namely obtains yeast culture thing after room temperature fragmentation rear mistake 200 micron pore size sieve.
Preferably, component and the mass fraction of described first liquid substratum are: bean cake powder 3-5 part, soyflour 2-4 part, bicarbonate of ammonia 0.05-0.4 part, glucose sugar 1-10 part, potassium primary phosphate 1.5-3 part, magnesium sulfate 0.1-1 part, manganous sulfate 0.1-0.8 part, Sodium Propionate 0.1-0.5 part, ammonium nitrate 0.1-0.4 part, four water eight Sodium Tetraborate 3-10 parts, sterilized water 80-90 part.
Preferably, the component of described second liquid substratum and mass fraction are: N.F,USP MANNITOL 8-10 part, bean cake powder 5-10 part, glucose sugar 1-5 part, Semen Maydis powder 3-5 part, potassium primary phosphate 1-5 part, SODIUM PHOSPHATE, MONOBASIC 0.2-0.9 part, magnesium sulfate 1-2 part, sterilized water 50-80 part.
Preferably, described microbe carrier is plant ash, wilkinite or both mixtures.
The present invention's four kinds of bacterial classifications used are subtilis (Bacillus subtilis), bacillus megaterium (Bacillus megaterium), root nodule bacterium (Root nodule bacteria).
The preparation method of composite fertilizer is: in proportion by dry cattle and sheep excrement, urea, calcium superphosphate, bamboo charcoal powder, boric acid, ferrous sulfate, zinc sulfate, Sodium Selenite, potassium primary phosphate, put into stirrer stirring after ammonium molybdate mixing and after 100 minutes, put into pulverizer pulverizing 50 minutes, take out after pulverizing and add subtilis culture by proportioning, bacillus megaterium culture, put into stirrer after rhizobia cultures and continue stirring 100 minutes, pour pattern grit machine after stirring completes into and be produced into hard particles, cross 400 micron pore size sieves, the composite fertilizer of tartary wheet flavone content must be improved.
Advantage of the present invention is:
1. composite fertilizer of the present invention can significantly improve flavones content in buckwheat seed, improves the nutritional health function of buckwheat, the economic benefit of improve buckwheat plantation, producing.
2. composite fertilizer of the present invention nutritive element enriches, and can promote that buckwheat grows, strengthen buckwheat resistibility, reach the beneficial effect of increasing both production and income disease resistance.
3. fertilizer of the present invention contains microbial preparation, and profitable strain quantity is up to 10
9/ g, and containing various active metabolic substd, can Soil structure be improved, decompose remaining agricultural chemicals in soil and crop, improve crop and the absorption of nutrient is changed, improve economy and zoology benifit.
4. the present invention contains the carrier with special adsorption function, improves in the adsorptivity of fertilizer on soil and blade face, effectively improves the micro-ecological environment on crop root and blade face, reach a kind of safe, green planting effect.
5. cost of the present invention is low, and source is wide, and product safety is reliable.
Embodiment:
Describe the present invention in detail below in conjunction with specific embodiment, be used for explaining the present invention in this illustrative examples of inventing and explanation, but not as a limitation of the invention.
Embodiment one
Improve a composite fertilizer for tartary wheet flavone content, its component and parts by weight comprise: 120 parts, dry cattle and sheep excrement, 8 parts, urea, calcium superphosphate 5 parts, bamboo charcoal powder 2 parts, boric acid 1 part, 1 part, ferrous sulfate, 1 part, zinc sulfate, Sodium Selenite 1 part, potassium primary phosphate 0.5 part, ammonium molybdate 0.1 part, subtilis 1 part, bacillus megaterium 3 parts, root nodule bacterium 2 parts.
Wherein the culturing step of its subtilis, bacillus megaterium, root nodule bacterium is:
The first step: the 120 DEG C of sterilizings of first liquid substratum were mixed with weight ratio 100:1 with tween after 20 minutes, subtilis, bacillus megaterium mixture are inoculated in above-mentioned substratum according to the ratio of weight ratio 1:100, abundant stirring airtight standing for fermentation 7 days after 10 minutes, sampling Detection thalli growth situation and measure pH value, plate count, when viable bacteria total concn is more than 10
9/ ML and pH value just stop fermentation 3.0;
Second step: the 120 DEG C of sterilizings of second liquid substratum were ventilated after 20 minutes, root nodule bacterium are inoculated in second liquid substratum according to weight ratio 1:100 ratio, abundant stirring airtight standing for fermentation 4 days after 10 minutes, sampling Detection thalli growth situation and measure pH value, plate count, when viable bacteria total concn is more than 10
9namely/ML stops fermentation;
3rd step: by the first step with 35 DEG C of warm air dryings after under second step gained culture relative centrifugal force 3000 × g centrifugal 10 minutes 10 hours, make water content be reduced to less than 25%;
4th step: the microbial strain culture that the 3rd step obtains mixed according to 1:100 part by weight with plant ash, gets product after room temperature fragmentation rear mistake 200 micron pore size sieve.
In above-mentioned steps, the component of first liquid substratum and mass fraction are: bean cake powder 3 parts, soyflour 2 parts, 0.05 part, bicarbonate of ammonia, glucose sugar 1 part, potassium primary phosphate 1.5 parts, 0.1 part, magnesium sulfate, manganous sulfate 0.1 part, Sodium Propionate 0.1 part, 0.1 part, ammonium nitrate, four water eight Sodium Tetraborate 3 parts, sterilized water 80 parts; The component of second liquid substratum and mass fraction are: 8 parts, N.F,USP MANNITOL, bean cake powder 5 parts, glucose sugar 1 part, Semen Maydis powder 3 parts, potassium primary phosphate 1 part, SODIUM PHOSPHATE, MONOBASIC 0.2 part, 1 part, magnesium sulfate, sterilized water 50 parts.
Embodiment two
Improve a composite fertilizer for tartary wheet flavone content, it is characterized in that: composite fertilizer's component and parts by weight comprise: 150 parts, dry cattle and sheep excrement, 20 parts, urea, calcium superphosphate 10 parts, bamboo charcoal powder 10 parts, boric acid 5 parts, 2 parts, ferrous sulfate, 2 parts, zinc sulfate, Sodium Selenite 2 parts, potassium primary phosphate 2 parts, ammonium molybdate 0.5 part, subtilis 3 parts, bacillus megaterium 5 parts, root nodule bacterium 6 parts.
The culturing step of subtilis, bacillus megaterium, root nodule bacterium is:
The first step: the 120 DEG C of sterilizings of first liquid substratum were mixed with weight ratio 100:1 with tween after 20 minutes, subtilis, bacillus megaterium mixture are inoculated in above-mentioned substratum according to the ratio of weight ratio 1:100, abundant stirring airtight standing for fermentation 4 days after 10 minutes, sampling Detection thalli growth situation and measure pH value, plate count, when viable bacteria total concn is more than 10
9/ ML and pH value just stop fermentation 4.0;
Second step: the 120 DEG C of sterilizings of second liquid substratum were ventilated after 20 minutes, root nodule bacterium are inoculated in second liquid substratum according to weight ratio 1:100 ratio, abundant stirring airtight standing for fermentation 3 days after 10 minutes, sampling Detection thalli growth situation and measure pH value, plate count, when viable bacteria total concn is more than 10
9namely/ML stops fermentation;
3rd step: by the first step with 40 DEG C of warm air drying 10-20 hour after under second step gained culture relative centrifugal force 4000 × g centrifugal 30 minutes, make water content be reduced to less than 25%;
4th step: the microbial strain culture that the 3rd step obtains mixed according to 1:100 part by weight with wilkinite, gets product after room temperature fragmentation rear mistake 200 micron pore size sieve.
The component of first liquid substratum and mass fraction are: bean cake powder 3 parts, soyflour 2 parts, 0.4 part, bicarbonate of ammonia, glucose sugar 1 part, potassium primary phosphate 3 parts, 1 part, magnesium sulfate, manganous sulfate 0.8 part, Sodium Propionate 0.5 part, 0.4 part, ammonium nitrate, four water eight Sodium Tetraborate 10 parts, sterilized water 90 parts; The component of second liquid substratum and mass fraction are: 8 parts, N.F,USP MANNITOL, bean cake powder 5 parts, glucose sugar 1 part, Semen Maydis powder 5 parts, potassium primary phosphate 1 part, SODIUM PHOSPHATE, MONOBASIC 0.9 part, 2 parts, magnesium sulfate, sterilized water 80 parts.
Embodiment three
Improve a composite fertilizer for tartary wheet flavone content, it is characterized in that: composite fertilizer's component and parts by weight comprise: 125 parts, dry cattle and sheep excrement, 15 parts, urea, calcium superphosphate 8 parts, bamboo charcoal powder 8 parts, boric acid 4 parts, 2 parts, ferrous sulfate, 2 parts, zinc sulfate, Sodium Selenite 1 part, potassium primary phosphate 1 part, ammonium molybdate 0.1 part, subtilis culture 1 part, bacillus megaterium culture 5 parts, rhizobia cultures 6 parts.
The culturing step of subtilis, bacillus megaterium, root nodule bacterium is:
The first step: the 120 DEG C of sterilizings of first liquid substratum were mixed with weight ratio 100:1 with tween after 20 minutes, subtilis, bacillus megaterium mixture are inoculated in above-mentioned substratum according to the ratio of weight ratio 1:100, abundant stirring airtight standing for fermentation 5 days after 10 minutes, sampling Detection thalli growth situation and measure pH value, plate count, when viable bacteria total concn is more than 10
9/ ML and pH value just stop fermentation 4.0;
Second step: the 120 DEG C of sterilizings of second liquid substratum were ventilated after 20 minutes, root nodule bacterium are inoculated in second liquid substratum according to weight ratio 1:100 ratio, abundant stirring airtight standing for fermentation 4 days after 10 minutes, sampling Detection thalli growth situation and measure pH value, plate count, when viable bacteria total concn is more than 10
9namely/ML stops fermentation;
3rd step: by the first step with 40 DEG C of warm air dryings after under second step gained culture relative centrifugal force 3500 × g centrifugal 10 minutes 20 hours, make water content be reduced to less than 25%;
4th step: the microbial strain culture that the 3rd step obtains mixed according to 1:100 part by weight with plant ash, get product after room temperature fragmentation rear mistake 200 micron pore size sieve microbial strain culture.
The component of first liquid substratum and mass fraction are: bean cake powder 5 parts, soyflour 4 parts, 0.4 part, bicarbonate of ammonia, glucose sugar 8 parts, potassium primary phosphate 3 parts, 1 part, magnesium sulfate, manganous sulfate 0.8 part, Sodium Propionate 0.4 part, 0.4 part, ammonium nitrate, four water eight Sodium Tetraborate 10 parts, sterilized water 90 parts; The component of second liquid substratum and mass fraction are: 10 parts, N.F,USP MANNITOL, bean cake powder 8 parts, glucose sugar 5 parts, Semen Maydis powder 4 parts, potassium primary phosphate 3 parts, SODIUM PHOSPHATE, MONOBASIC 0.3 part, 2 parts, magnesium sulfate, sterilized water 50 parts.
Embodiment four
Improve a composite fertilizer for tartary wheet flavone content, it is characterized in that: composite fertilizer's component and parts by weight comprise: 120 parts, dry cattle and sheep excrement, 10 parts, urea, calcium superphosphate 10 parts, bamboo charcoal powder 7 parts, boric acid 4 parts, 1 part, ferrous sulfate, 1 part, zinc sulfate, Sodium Selenite 1 part, potassium primary phosphate 2 parts, ammonium molybdate 0.5 part, subtilis 3 parts, bacillus megaterium 4 parts, root nodule bacterium 5 parts.
The culturing step of subtilis, bacillus megaterium, root nodule bacterium is:
The first step: the 120 DEG C of sterilizings of first liquid substratum were mixed with weight ratio 100:1 with tween after 20 minutes, subtilis, bacillus megaterium mixture are inoculated in above-mentioned substratum according to the ratio of weight ratio 1:100, abundant stirring airtight standing for fermentation 7 days after 10 minutes, sampling Detection thalli growth situation and measure pH value, plate count, when viable bacteria total concn is more than 10
9/ ML and pH value just stop fermentation 4.0;
Second step: the 120 DEG C of sterilizings of second liquid substratum were ventilated after 20 minutes, root nodule bacterium are inoculated in second liquid substratum according to weight ratio 1:100 ratio, abundant stirring airtight standing for fermentation 3 days after 10 minutes, sampling Detection thalli growth situation and measure pH value, plate count, when viable bacteria total concn is more than 10
9namely/ML stops fermentation;
3rd step: by the first step with 40 DEG C of warm air dryings after under second step gained culture relative centrifugal force 3000 × g centrifugal 30 minutes 10 hours, make water content be reduced to less than 25%;
4th step: the microbial strain culture that the 3rd step obtains mixed according to 1:100 part by weight with wilkinite, gets product after room temperature fragmentation rear mistake 200 micron pore size sieve.
The component of first liquid substratum and mass fraction are: bean cake powder 4 parts, soyflour 3 parts, 0.4 part, bicarbonate of ammonia, glucose sugar 9 parts, potassium primary phosphate 3 parts, 1 part, magnesium sulfate, manganous sulfate 0.7 part, Sodium Propionate 0.4 part, 0.3 part, ammonium nitrate, four water eight Sodium Tetraborate 10 parts, sterilized water 85 parts; The component of second liquid substratum and mass fraction are: 9 parts, N.F,USP MANNITOL, bean cake powder 9 parts, glucose sugar 4 parts, Semen Maydis powder 4 parts, potassium primary phosphate 4 parts, SODIUM PHOSPHATE, MONOBASIC 0.8 part, 1 part, magnesium sulfate, sterilized water 60 parts.
The preparation method of composite fertilizer is: put into pulverizer pulverizing 50 minutes by putting into after stirrer stirs 100 minutes after dry cattle and sheep excrement, urea, calcium superphosphate, bamboo charcoal powder, boric acid, ferrous sulfate, zinc sulfate, Sodium Selenite, potassium primary phosphate, ammonium molybdate mixing in proportion; put into stirrer after taking-up adds subtilis, bacillus megaterium, root nodule bacterium by proportioning after pulverizing and continue stirring 100 minutes; pour pattern grit machine after stirring completes into and be produced into hard particles; cross 400 micron pore size sieves, the composite fertilizer of tartary wheet flavone content must be improved.
Composite fertilizer's using method: composite fertilizer of the present invention is the buckwheat knot fringe initial stage best use age, and every mu of usage quantity is 30-50 kilogram, and hand is grabbed and spread.
Known by contrasting, the buckwheat seed of this composite fertilizer practical, flavones content significantly improves 40-60%, increases the economic benefit of buckwheat.
Above-described embodiment is illustrative principle of the present invention and effect thereof only, but not for limiting the present invention.Any person skilled in the art scholar all without prejudice under spirit of the present invention and category, can modify above-described embodiment or changes.Therefore, such as have in art usually know the knowledgeable do not depart from complete under disclosed spirit and technological thought all equivalence modify or change, must be contained by claim of the present invention.
Claims (7)
1. improve a composite fertilizer for tartary wheet flavone content, it is characterized in that: its component and parts by weight comprise: dry cattle and sheep excrement 100-150 part, urea 5-20 part, calcium superphosphate 1-10 part, bamboo charcoal powder 1-10 part, boric acid 1-5 part, ferrous sulfate 1-2 part, zinc sulfate 1-2 part, Sodium Selenite 1-2 part, potassium primary phosphate 0.5-2 part, ammonium molybdate 0.1-0.5 part.
2. a kind of composite fertilizer of improving tartary wheet flavone content according to claim 1, is characterized in that: its component and parts by weight comprise: dry cattle and sheep excrement 120-150 part, urea 8-20 part, calcium superphosphate 5-10 part, bamboo charcoal powder 2-10 part, boric acid 1-5 part, ferrous sulfate 1-2 part, zinc sulfate 1-2 part, Sodium Selenite 1-2 part, potassium primary phosphate 0.5-2 part, ammonium molybdate 0.1-0.5 part.
3. a kind of composite fertilizer of improving tartary wheet flavone content according to claim 1 and 2, it is characterized in that: its component also comprises subtilis culture, bacillus megaterium culture and rhizobia cultures, parts by weight are respectively 1-3 part, 3-5 part and 2-6 part.
4. a kind of composite fertilizer of improving tartary wheet flavone content according to claim 3, is characterized in that: the culturing step of its subtilis culture, bacillus megaterium culture and rhizobia cultures is:
The first step: the 120 DEG C of sterilizings of first liquid substratum were mixed with weight ratio 100:1 with tween after 20 minutes, subtilis, bacillus megaterium mixture are inoculated in above-mentioned substratum according to the ratio of weight ratio 1:100, abundant stirring airtight standing for fermentation 4-7 days after 10 minutes, sampling Detection thalli growth situation and measure pH value, plate count, when viable bacteria total concn is more than 10
9/ ML and pH value just stop fermentation at 3.0-4.0;
Second step: the 120 DEG C of sterilizings of second liquid substratum were ventilated after 20 minutes, root nodule bacterium are inoculated in second liquid substratum according to weight ratio 1:100 ratio, abundant stirring airtight standing for fermentation 1-4 days after 10 minutes, sampling Detection thalli growth situation and measure pH value, plate count, when viable bacteria total concn is more than 10
9namely/ML stops fermentation;
3rd step: by the first step with 35-40 DEG C of warm air drying 10-20 hour after under second step gained culture relative centrifugal force 3000-4000 × g centrifugal 10-30 minute, make water content be reduced to less than 25%;
4th step: the microbial strain culture that the 3rd step obtains mixed according to 1:100 part by weight with microbe carrier, namely obtains yeast culture thing after room temperature fragmentation rear mistake 200 micron pore size sieve.
5. described a kind of composite fertilizer of improving tartary wheet flavone content according to claim 4, it is characterized in that: component and the mass fraction of described first liquid substratum are: bean cake powder 3-5 part, soyflour 2-4 part, bicarbonate of ammonia 0.05-0.4 part, glucose sugar 1-10 part, potassium primary phosphate 1.5-3 part, magnesium sulfate 0.1-1 part, manganous sulfate 0.1-0.8 part, Sodium Propionate 0.1-0.5 part, ammonium nitrate 0.1-0.4 part, four water eight Sodium Tetraborate 3-10 parts, sterilized water 80-90 part.
6. described a kind of composite fertilizer of improving tartary wheet flavone content according to claim 4, is characterized in that: component and the mass fraction of described second liquid substratum are: N.F,USP MANNITOL 8-10 part, bean cake powder 5-10 part, glucose sugar 1-5 part, Semen Maydis powder 3-5 part, potassium primary phosphate 1-5 part, SODIUM PHOSPHATE, MONOBASIC 0.2-0.9 part, magnesium sulfate 1-2 part, sterilized water 50-80 part.
7. described a kind of composite fertilizer of improving tartary wheet flavone content according to claim 4, is characterized in that: described microbe carrier is plant ash, wilkinite or both mixtures.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107043287A (en) * | 2017-05-15 | 2017-08-15 | 福建农林大学 | A kind of high flavones accumulation special complex fertilizer of euscaphis konishii |
| CN108947602A (en) * | 2018-09-28 | 2018-12-07 | 郭文平 | A kind of Tartary buckwheat biological organic fertilizer and production method using the fermentation of tea tree branch |
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| CN102276324A (en) * | 2010-06-12 | 2011-12-14 | 闫家良 | Preparation method for buckwheat yield increasing fertilizer |
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| CN107043287A (en) * | 2017-05-15 | 2017-08-15 | 福建农林大学 | A kind of high flavones accumulation special complex fertilizer of euscaphis konishii |
| CN108947602A (en) * | 2018-09-28 | 2018-12-07 | 郭文平 | A kind of Tartary buckwheat biological organic fertilizer and production method using the fermentation of tea tree branch |
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