CN104480076B - The production method of anaerobism and oxygen probiotics symbiosis metabolism enzyme preparation - Google Patents

The production method of anaerobism and oxygen probiotics symbiosis metabolism enzyme preparation Download PDF

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Publication number
CN104480076B
CN104480076B CN201410753766.7A CN201410753766A CN104480076B CN 104480076 B CN104480076 B CN 104480076B CN 201410753766 A CN201410753766 A CN 201410753766A CN 104480076 B CN104480076 B CN 104480076B
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matrix
anaerobism
switch
fermentation
probiotics
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CN104480076A (en
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王胧庆
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Zhejiang Senjing Biotechnology Co ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes

Abstract

The invention discloses the production methods that anaerobism and oxygen probiotics symbiosis are metabolized enzyme preparation, include the following steps:First set refrigeration cold-room, anaerobic fermentating groove and circulation constant temperature heating device, the rear end automatic storage fermentation tank of matrix, wherein the outflow port of anaerobic fermentating groove is free surface liquid stream mouth, 1) fresh cultured matrix stirred in refrigeration cold-room, cooled down and maintain temperature, then the matrix is squeezed at regular time and quantity in the anaerobic fermentating groove, while thermostatic control is carried out to anaerobic fermentating groove by circulating heater;2) after the matrix enters anaerobic fermentating groove, free surface height of the liquid biological enzyme that fermentation is completed because being limited to outflow port, naturally it is spilled over in the rear end automatic storage fermentation tank, the liquid biological enzyme of stream is just gone out in the case of still active, the stable can through rear end setting carries out the secondary fermentation of product.The advantages of present invention is grown with excluding pathogenic bacteria, shortening probiotics screening time, raising culture efficiency, bacterium phase and stable quality.

Description

The production method of anaerobism and oxygen probiotics symbiosis metabolism enzyme preparation
Technical field
The present invention relates to the technical field that probiotics symbiosis is metabolized the production method of enzyme preparation, specifically a kind of anaerobism is simultaneous Oxygen probiotics symbiosis is metabolized the technology of the production method of enzyme preparation.
Background technology
Tradition culture anaerobism or aerobic beneficial bacterium method be single kind culture after mixed according to certain proportion, i.e., Batch fermentation, it is not only time-consuming in making, and still need to undertake the contaminated problem of strain in manufacturing process, cause price high Expensive, operation is not easy.
Invention content
For overcome the deficiencies in the prior art, the object of the present invention is to provide a kind of growths of excluding pathogenic bacteria, shorten prebiotic Bacterium screening time improves culture efficiency, the producer of the anaerobism and oxygen probiotics symbiosis metabolism enzyme preparation of bacterium phase and stable quality Method.
It is realized below by following technical scheme:
A kind of production method of anaerobism and oxygen probiotics symbiosis metabolism enzyme preparation, it is characterised in that:The anaerobism and oxygen benefit The production method of raw bacterium symbiosis metabolism enzyme preparation includes the following steps:First set refrigeration cold-room, the anaerobism of matrix fixed temperature Fermentation tank and circulation constant temperature heating device, rear end automatic storage fermentation tank, between refrigeration cold-room and the anaerobic fermentating groove mutually Connection is interconnected between anaerobic fermentating groove and rear end automatic storage fermentation tank, and wherein the outflow port of anaerobic fermentating groove is freely Liquid level liquid stream mouth, 1) fresh cultured matrix is stirred in refrigeration cold-room, cools down and maintains temperature, then by matrix timing It quantitatively squeezes into the anaerobic fermentating groove, while thermostatic control is carried out to anaerobic fermentating groove by circulating heater;2) described After matrix enters anaerobic fermentating groove, the free surface height of the liquid biological enzyme of completion of fermenting because being limited to outflow port is overflow naturally Go out to the rear end automatic storage fermentation tank, has just gone out the liquid biological enzyme of stream in the case of still active, be arranged through rear end Stable can carry out product secondary fermentation.
The enzyme fermentation liquid of the speed of the fresh cultured matrix being added into anaerobic fermentating groove and anaerobic fermentating groove outflow Speed it is equal, so that liquid measure in anaerobic fermentating groove is remained constant.
The source of cold temperature needed for the matrix include freezer, refrigerator-freezer or frozen water machine with gas or liquid form by freezing Compressor operating reaches the cold temperature.
The preparation method of the fresh cultured matrix:Raw water pure water is obtained by reverse osmose pure-water equipment first to respectively enter One ice water tank enters the agitator in cold-room after ice water tank refrigeration machine reduces water temperature, while microorganism is grown required nutrient Molasses are squeezed into agitator with nutritive salt and are stirred to scale, and the matrix storage barrel in cold-room is squeezed into pumping later, enters back into urgency Cooling preservation is carried out in quickly cooling tank, avoids molasses matrix acidifying, and matrix after cooling is transported to cold-room holding vessel etc. again later To be input to anaerobic fermentating groove.
Constant temperature and defined acid-base value and oxidation-reduction potential are maintained when the anaerobic fermentating groove is fermented, when matrix not It is disconnected that acid-base value PH in whole fermentation tank will be continued to decline when injecting the fermentation tank at regular time and quantity and carrying out anaerobic fermentation, In order to maintain suitable for anaerobism and facultative microbe living environment, when the PH in fermentation tank is too low, pass through the storage that startup stores lye Tank motor is deposited, the lye configured is squeezed into fermentation tank, to maintain PH in fixed range.
Required temperature follows wound, oily heating by direct or indirect electrothermal tube modular, heating wire in the fermentation tank Type, solar energy heating, Boiler Steam heating or the heat exchange of compressor condenser and obtain.
Required PH touches the quantitative timing matrix automated with display from console PLC in the fermentation tank and buck supplies It is deceleration Water-pumping motor with time controller, electromagnetism to maintain normal fermentation to require pH value, conveying quantitative timing equipment to fermentation tank Valve, peristaltic pump dispose one to wait pumping case and go out to flow tank and multiple stable storing tanks composition institute in the rear end of the fermentation tank outflow port Rear end automatic storage fermentation tank is stated, reaches specified amount when going out to flow the liquid state fermentation enzyme in tank time pumping case, delivery pump will be started automatically Pu is squeezed into stable storing tank and is stored.
The time takes out case and goes out to flow in the time pumping case of tank equipped with water pumper operating switch is started automatically, and the switch is opened for floating ball Pass or water level switch;Or optoelectronic switch;Or weight switch;Or close switch;Or microswitch;Or flow switch;Or number is opened It closes.
The marquis takes out case and pumps to stable fill by switching as float switch or water level switch automatically;Or optoelectronic switch;Or Weight switch;Or close switch;Or microswitch;Or flow switch;Or number switch control solenoid valve is turned on and off.
The fermentate for completing storage and sediment are evacuated to stabilization by the stabilization filling propeller, gear motor and frequency converter Abhiseca is recycled, or the fermentate for completing storage is evacuated to stable abhiseca with sediment equipped with suction pump stablizing filling bottom It is recycled.
Compared with the prior art, the present invention has the following advantages.
The present invention is due to using the mode continuously fermented, that is, using continous way bacteria group culture, by sealed fermenting Microorganism Natural Selection mechanism obtains anaerobism and aerobic probiotics in slot, and reaches microorganism by continuous feed and environmental Kuznets Curves The growth of life and growth in nature, therefore the present invention more shortens probiotics screening time and improves other than excluding pathogenic bacteria growth problems Culture efficiency, bacterium phase and stable quality.
Anaerobism of the present invention and oxygen probiotics symbiosis metabolism enzyme preparation production routine are will be certain by quantitative timing control Speed, which is injected, adds fresh culture in fermentation tank, while flowing out enzyme fermentation liquid with identical speed, to make the liquid in fermentation tank Amount maintains constant fermentation process.Since multiple growth cycles and multifarious microorganism growth need to be completed in fermentation tank, because This this zymotechnique flow need to carry out the scientific quantitative and qualitative with automation and control, due to using anaerobic fermentation tank and anaerobism and Aerobic Pseudomonas natural selection method carrys out symbiosis, therefore last zymotic fluid fully belongs to rich and varied dominant microflora, therefore compares it For his fermentation method, strain bacterium is mutually stablized, and is not easy to degenerate, and products application range is wide, production cost relative moderate, and matrix molasses turn The utilization rate for being melted into product is high with transformation efficiency, and since the utilization ratio to matrix is higher, production concentration is also for example criticized than other Secondary fermentation wants high.In the case where keeping a long-term stable state, the utilization rate and yield in unit time of raising equipment can be passed through. Each parameter tends to constant to the present invention during the fermentation, convenient for automatically controlling, especially to the material that is added during feed supplement without Bacterium requirement is low, incomplete in fermentation, and anaerobic fermentation can be reached again by feeding number by reduction, be not required to whole works Useless, ferment tank switching.
Description of the drawings
Fig. 1 is the production method flow chart that anaerobism of the present invention and oxygen probiotics symbiosis are metabolized enzyme preparation.
Specific implementation mode
Anaerobism of the present invention and oxygen probiotics symbiosis are metabolized enzyme preparation production method, i.e. anaerobism and oxygen probiotics symbiosis metabolic enzyme Program of continuously fermenting, wherein comprising a kind of matrix refrigerator of special heat preservation, a kind of anaerobic fermentating groove, a kind of cycle, which is kept the temperature, to be added Hot device and rear end holding vessel, the technical process take automated production, each technique unit to be interconnected, and reach automation and connect Supervention ferment.Wherein anaerobic fermentating groove can also be anaerobic fermentation tank.Rear end holding vessel is also referred to as gutter.
The present invention refrigerates interior be equipped with of cold-room and matrix stirring, cooling and long-time is quickly maintained temperature, will by controller Matrix squeeze at regular time and quantity tool anaerobism with and oxygen probiotics fermentation tank, while by circulating-heating machine to fermentation tank fermentation tank into Row thermostatic control, the design of fermentation tank outflow port is picked up from by overflowing, after matrix enters fermentation tank, the liquid biological enzyme for completion of fermenting Because being limited to the free surface height of outflow port, it will be spilled over to rear end fermentation tank naturally, due to just going out the liquid biological enzyme of stream still It is active, therefore multiple stable cans are designed to carry out product stability program, i.e. secondary fermentation in rear end.
The continuous production processes of the present invention including matrix agitator tank, are rapidly dropped by integrating matrix continuous feed unit Warm tank stablizes low temperature cold-room, intake line, anaerobic fermentating groove unit, is equipped with free surface liquid stream mouth, cycle heat-insulation unit with Storage element, it is interior to be equipped with automatic subpackaging pipeline and automatic circulating system.The present invention can be bred through 5 to 7 days pot-lifes A large amount of anaerobism and oxygen dominant bacteria, while being metabolized the enzyme needed for birth object growth, trace element, vitamin, Amino acid etc..
Present invention is further described in detail below in conjunction with the accompanying drawings.
Such as Fig. 1, it is equipment arrangement schematic diagram, also shows the flow chart for program of continuously fermenting, illustrated in figure Office 1, control laboratory 2, raw material storage 3, agitator 4, storage barrel 5, buck slot 6, marquis take out case 7, discharging motor 8, circulation pipe 9, motor 10, gutter 11, discharge port 12, water inlet pvc pipe 13, feed pipe 14, heating machine 15, frozen water machine 16, compressor are recycled 17, reacting furnace 18, drainage position 19.Wherein each gutter 11 is equipped with discharge port 12, and each gutter 11 passes through feed pipe 14 and marquis It takes out case 7 to be connected, marquis, which takes out, is equipped with discharging motor 8 at case 7.First by raw water by reverse osmose pure-water equipment obtain pure water respectively into Enter ice water tank, the agitator matrix entered in cold-room after ice water tank refrigeration machine reduces water temperature preserves the agitator tank in cold-room, together When by microorganism grow needed for nutrient molasses squeeze into agitator to scale with nutritive salt and be stirred, rear side Pu squeeze into cold-room Storage barrel rapidly cold tank carries out cooling preservation, avoid molasses matrix acidifying, the matrix after cooling be transported to cold-room again later Holding vessel, that is, storage barrel etc. is to be input to fermentation tank.Cold temperature source needed for matrix of the present invention includes freezer, refrigerator-freezer or ice water Machine reaches cold temperature with gas or liquid form by refrigeration compressor operation.The agitator of molasses and nutritive salt completes mixing water Pump is conveyed into matrix storage barrel, and rapidly cold tank carries out cooling preservation, avoids molasses matrix acidifying, is slowed down using propeller or cooperation Machine or cooperation frequency converter are cooled with the stirring of rational speed, and anaerobic fermentating groove, which carries out fermentation, need to maintain constant temperature, acid-base value, with oxidation Reduction potential, when matrix constantly injects fermentation tank at regular time and quantity and carries out anaerobic fermentation, the acid-base value PH in whole fermentation tank It will continue to decline, in order to maintain suitable for anaerobism and facultative microbe living environment, when the PH in fermentation tank is too low, system will The holding vessel motor for starting storage lye, squeezes into fermentation tank, to maintain PH in fixed range by the lye configured.Fermentation tank Interior required temperature, temperature source are followed wound by direct or indirect electrothermal tube modular, heating wire, and oil heating type, solar energy adds Heat, Boiler Steam heating, compressor condenser heat exchange.Required PH touches display by console PLC and matches in fermentation tank of the present invention It closes the quantitative timing matrix of automation and buck supplies fermentation tank maintenance normal fermentation requirement pH value, conveying quantitative timing equipment is Deceleration Water-pumping motor conveys tool movement with time controller, solenoid valve, peristaltic pump, all generated using motor.Fermentation tank goes out Stream mode is gone out using free liquid, after matrix injects fermentation tank, if slot inner height will go out when being more than outflow port height from flow liquid, And then the technique for reaching production of continuously fermenting.Disposing one to wait in fermentation tank outflow port rear end, pumping case goes out to flow tank and multiple storages are steady Determine tank i.e. storage element, reaches a certain amount of when going out to flow the liquid state fermentation enzyme in tank time pumping case, automatic will start to convey to help out with money and squeeze into Stable storing tank is stored.It is float switch or water level switch, photoelectricity to start water pumper work automatically in present invention time pumping case Switch, weight switch, close to switch, microswitch, flow switch, number switch.Marquis's pumping case pumps to gutter and fills automatically By float switch or water level switch, optoelectronic switch, weight switch, close to switch, microswitch, flow switch, number switch etc. Switch control solenoid valve is turned on and off.Operation, marquis take out case and come out delivery pipe.Gutter has species precipitate situation with slot bottom, To keep uniform, gear motor and frequency converter are closed with propeller, or the fermentate of storage will be completed with suction pump with trench bottom Groove top is evacuated to sediment to be recycled.

Claims (9)

1. the production method of a kind of anaerobism and oxygen probiotics symbiosis metabolism enzyme preparation, it is characterised in that:The anaerobism and oxygen are prebiotic The production method of bacterium symbiosis metabolism enzyme preparation includes the following steps:First set refrigeration cold-room, the anaerobism hair of matrix fixed temperature Ferment slot and circulation constant temperature heating device, rear end automatic storage fermentation tank, mutually interconnect between refrigeration cold-room and the anaerobic fermentating groove It is logical, it is interconnected between anaerobic fermentating groove and rear end automatic storage fermentation tank, wherein the outflow port of anaerobic fermentating groove is free liquid Face liquid stream mouth,
1) fresh cultured matrix stirred in refrigeration cold-room, cooled down and maintain temperature, then beat the matrix at regular time and quantity Enter in the anaerobic fermentating groove, while thermostatic control is carried out to anaerobic fermentating groove by circulating heater;
2) after the matrix enters anaerobic fermentating groove, the liquid biological enzyme of completion of fermenting is high because of the free surface for being limited to outflow port Degree, is spilled over in the rear end automatic storage fermentation tank, has just gone out the liquid biological enzyme of stream in the case of still active naturally, passes through The stable can of rear end setting carries out the secondary fermentation of product;
The speed of the speed of the fresh cultured matrix being added into anaerobic fermentating groove and the enzyme fermentation liquid of anaerobic fermentating groove outflow It spends equal, the liquid measure in anaerobic fermentating groove is made to maintain constant fermentation process.
2. the production method of anaerobism and oxygen probiotics symbiosis metabolism enzyme preparation according to claim 1, it is characterised in that:It is described The source of cold temperature needed for matrix includes that freezer, refrigerator-freezer or frozen water machine are reached with gas or liquid form by refrigeration compressor operation To the cold temperature.
3. the production method of anaerobism and oxygen probiotics symbiosis metabolism enzyme preparation according to claim 1, it is characterised in that described The preparation method of fresh cultured matrix:Raw water is obtained into pure water by reverse osmose pure-water equipment first and enters an ice water tank, through ice water Slot refrigeration machine, which reduces, enters the agitator in cold-room after water temperature, at the same by microorganism grow needed for nutrient molasses and nutritive salt according to than Example is squeezed into agitator and is stirred, and the matrix storage barrel in cold-room is squeezed into pumping later, is entered back into rapidly cold tank and is cooled down It preserves, avoids molasses matrix acidifying, matrix after cooling is transported to cold-room holding vessel etc. to be input to anaerobic fermentation again later Slot.
4. the production method of anaerobism and oxygen probiotics symbiosis metabolism enzyme preparation according to claim 1, it is characterised in that:It is described Constant temperature and defined acid-base value and oxidation-reduction potential are maintained when anaerobic fermentating groove is fermented, when matrix is constantly noted at regular time and quantity When entering the fermentation tank and carrying out anaerobic fermentation, the acid-base value PH in whole fermentation tank will be continued to decline, in order to remain suitable Anaerobism and facultative microbe living environment are stored the holding vessel motor of lye by startup, will matched when the PH in fermentation tank is too low The lye set squeezes into fermentation tank, to maintain PH in fixed range.
5. the production method of anaerobism and oxygen probiotics symbiosis metabolism enzyme preparation according to claim 1, it is characterised in that:It is described Required temperature follows wound, oil heating type by direct or indirect electrothermal tube modular, heating wire in fermentation tank, solar energy adds Heat, Boiler Steam heating or the heat exchange of compressor condenser and obtain.
6. the production method of anaerobism and oxygen probiotics symbiosis metabolism enzyme preparation according to claim 1, it is characterised in that:It is described Required PH touches the quantitative timing matrix automated with display from console PLC in fermentation tank and buck supply fermentation tank maintains It is that deceleration Water-pumping motor matches time controller, solenoid valve, peristaltic pump that normal fermentation, which requires pH value, conveying quantitative timing equipment,
It disposes one to wait pumping case in the rear end of the fermentation tank outflow port and goes out to flow tank and multiple stable storing tanks composition rear end Automatic storage fermentation tank reaches specified amount when going out to flow the liquid state fermentation enzyme in tank time pumping case, automatic will start to convey to pump and squeeze into Stable storing tank is stored.
7. the production method of anaerobism and oxygen probiotics symbiosis metabolism enzyme preparation according to claim 6, it is characterised in that:It is described It waits pumping case to go out to flow equipped with automatic startup water pumper operating switch in the time pumping case of tank, described switch is that float switch or water level are opened It closes;Or optoelectronic switch;Or weight switch;Or close switch;Or microswitch;Or flow switch;Or number switch.
8. the production method of anaerobism and oxygen probiotics symbiosis metabolism enzyme preparation according to claim 6, it is characterised in that:It is described Marquis takes out case and pumps to stable can automatically completely by switching as float switch or water level switch;Or optoelectronic switch;Or weight switch;Or Close to switch;Or microswitch;Or flow switch;Or number switch control solenoid valve is turned on and off.
9. the production method of anaerobism and oxygen probiotics symbiosis metabolism enzyme preparation according to claim 6, it is characterised in that:It is described The fermentate for completing storage and sediment are evacuated to stable can jacking row and followed by stable can propeller, gear motor and frequency converter Ring, or the fermentate for completing storage is evacuated to stable can jacking row with sediment and followed equipped with suction pump stablizing pot bottom Ring.
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CN103497890A (en) * 2013-09-26 2014-01-08 北京华都诗华生物制品有限公司 Perfusion culture system and method for bacteria fermentation tank
CN103555771A (en) * 2013-11-01 2014-02-05 中国科学院广州能源研究所 Production method and equipment for preparing ethanol through CO fermentation

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CN101402913B (en) * 2008-11-20 2011-12-21 南京工业大学 Apparatus and process for producing propionic acid with joint production of vitamin B12 with batch type zymotechnics
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Publication number Priority date Publication date Assignee Title
CN101368154A (en) * 2008-06-18 2009-02-18 华中农业大学 Continuous culture system and method for human intestinal canal flora
CN101709263A (en) * 2009-10-30 2010-05-19 南京工业大学 Chemostat continuous cultivation device and method for screening succinic acid mutant strain
CN103436447A (en) * 2011-12-31 2013-12-11 常州益菌加生物科技有限公司 Method for preparing probiotics
CN103497890A (en) * 2013-09-26 2014-01-08 北京华都诗华生物制品有限公司 Perfusion culture system and method for bacteria fermentation tank
CN103555771A (en) * 2013-11-01 2014-02-05 中国科学院广州能源研究所 Production method and equipment for preparing ethanol through CO fermentation

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