CN104212704B - Microbiological culture media dispenser, microbiological culture media compound method and method for filling - Google Patents
Microbiological culture media dispenser, microbiological culture media compound method and method for filling Download PDFInfo
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- CN104212704B CN104212704B CN201410428579.1A CN201410428579A CN104212704B CN 104212704 B CN104212704 B CN 104212704B CN 201410428579 A CN201410428579 A CN 201410428579A CN 104212704 B CN104212704 B CN 104212704B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M99/00—Subject matter not otherwise provided for in other groups of this subclass
Abstract
The invention belongs to microbe research development technique field, disclose a kind of microbiological culture media dispenser, comprise preparing tank, substratum bag and controller case, preparing tank is provided with feed-pipe, discharge nozzle, substratum is wrapped and is provided with water inlet pipe and rising pipe, the water inlet pipe of substratum bag connects water source, the feed-water end of substratum bag is provided with water inflow filter, the rising pipe of substratum bag is connected to the feed-pipe of preparing tank, heating unit and control unit is provided with in controller case, heating unit heats preparing tank, temperature sensor is provided with in preparing tank, temperature sensor is connected to control unit, control unit adjusts the temperature of culture medium solution in preparing tank in real time according to the feedback of temperature sensor, in preparing tank, agitator is set.The present invention, by newly-designed dispenser and compound method, makes the preparation of substratum reach more low nutrition material damage, less manpower intervention and realizes the preparation of comparatively large vol substratum fast.
Description
Technical field
The invention belongs to microbe research development technique field, particularly a kind of microbiological culture media dispenser, microbiological culture media compound method and method for filling.
Background technology
Substratum is the required nutritive substance specializing in microorganism growth, breeding, metabolism and Product formation of manual method preparation.Substratum is widely used in the Micro biological Tests of pharmaceutical biological product, food, makeup, disposable sanitary articles, and water examination and Clinical Laboratory work.In addition, the research of be also separated with animal and plant cells for related microorganism, cultivating, development and production field.In recent years, along with the development of biotechnology industry, the activity such as research and development, production, check analysis in these fields above-mentioned is more and more extensive, causes the demand of substratum to grow with each passing day, and also significantly improves the requirement of substratum quality.For meeting and adapting to this substratum consumption that sharply increases and promote and specification of quality, automatic equipment instrument specially for two important process links (preparation and packing) of medium preparing comes out thereupon, and be applied to specialized substratum manufacturing company, the finished product substratum of the commercialization of production; Or come into the Microbiological Lab of terminal user, improve the work effect of scientific research or reviewer.This quasi-instrument has automatization substratum packing instrument, automatization substratum dispenser etc.
Automatization substratum packing instrument is from the craft that substratum packing working link the instead of traditional time and effort consuming manual operation that " is down flat plate ", and automatic separated loading instrument not only separate-loading packaging is fast, and point dull and stereotyped standard taken on is standardized, point process of assembling unmanned.Automatic separated loading instrument significantly improves plate culture medium quality, and the accuracy of detected result based on plate culture medium, greatly saves manpower, time simultaneously, cost-saving.
Automatization substratum dispenser matches with automatization packing instrument, the back from packing substratum---preparation working link promotes medium preparing speed further, but on market, existing substratum dispenser to still have in working efficiency in lifting substratum constant product quality and promoting and manyly has problem not solve.
Existing automatization substratum dispenser has the following disadvantages:
1. existing automatization substratum dispenser is all adopt the sterilizing of steam high temperature mode.High-temp steam sterilizing destroys relatively more serious to the nutritive substance in substratum, and destructiveness increases with vapor pressure (temperature) rising and time lengthening.The destructiveness (see Zhao Desheng, Korean clear, Zhang Mingdou, the selection of medium sterilization temperature and time, value engineering, 2010(5) of high-temp steam sterilizing to nutritive substance is described: 205-206) in table 1 and 2;
Table 1. steam sterilizing temperature, dependency between time and nutritive substance destructiveness
Table 2. steam sterilizing temperature, time are to the destructiveness of different saccharide solution;
2. existing automatization substratum dispenser mainly solves the automatization of medium sterilization process, and heat up by substratum, maintain sterilising temp, cool to the process of packing and carried out automated operation, user can work out temperature variation curve in time.But the process for preparation of substratum also comprises the step such as weighing, dissolving, mixing, adjustment pH of medicine, these steps also need manual operation to complete in existing automatization substratum dispenser uses, these steps are that most time-consuming, consumption are artificial, and the most easily occur weighing, the link of metering or miscount;
3. the substratum starting material packaging of existing market being sold and specification mostly be 250 grams or 500 grams by weight or packaging vessel capacity come bottle or the bag of metering, distributing, packaging.The starting material of this specification packaging, can not be finished in a short time in most of Microbiological Lab, one bottle of substratum starting material often wants repeated multiple times use, sometimes even will across several season or several year, during this, because each opens packaging, cause the problem such as the substratum starting material moisture absorption, caking, rotten, variable color, crossed contamination between infested, different reagent and bottle when weighing.And the substratum quality product finally causing different times to prepare is unstable, fluctuation is very large, make assay inaccurate, reliability reduces, and between different experiments room, scientific data comparability is poor;
4. powdered medium starting material weigh and also easily produce dust in transfer process, can pollute on the one hand weighing apparatus and to the high production of purity requirements or scientific research environment, another aspect also can be sucked by operator, causes allergy or discomfort, health risk;
5. equipment price is expensive, and existing automatization substratum dispenser price of commercially selling is all twenty or thirty more than ten thousand yuans, and many Microbiological Labs limit due to fund, cannot adopt.
Summary of the invention
In order to solve the problems of the technologies described above, the invention provides a kind of microbiological culture media dispenser, microbiological culture media compound method and method for filling, be intended to develop more low nutrition material damage, less manpower intervention and realize device for formulating and the preparing and packaging method of comparatively large vol substratum fast.
The technical scheme that the present invention takes is:
A kind of microbiological culture media dispenser, it is characterized in that, comprise preparing tank, substratum bag and controller case, described preparing tank is provided with feed-pipe, discharge nozzle, described substratum is wrapped and is provided with water inlet pipe and rising pipe, the water inlet pipe of described substratum bag connects water source, the feed-water end of described substratum bag is provided with water inflow filter, the rising pipe of described substratum bag is connected to the feed-pipe of described preparing tank, heating unit and control unit is provided with in described controller case, described heating unit heats described preparing tank, temperature sensor is provided with in described preparing tank, described temperature sensor is connected to described control unit, described control unit adjusts the temperature of culture medium solution in described preparing tank in real time according to the feedback of described temperature sensor, in described preparing tank, agitator is set, described agitator is controlled to stir the culture medium solution in described preparing tank by the control unit of described controller case.
Further, described controller case comprises interchanger, interchanger water-in, interchanger water outlet and water port, the interchanger water-in of described controller case is connected to described water source, the interchanger water outlet of described controller case is connected to the water inlet pipe of described substratum bag, and described control unit controls the water outlet of described interchanger water outlet or described water port draining according to the output water temperature of described interchanger.
Further, described preparing tank is provided with chuck, at the sidewall of the chuck of described preparing tank, chuck water-in and chuck water outlet are set, in described controller case, heating in water bath case is also set, described chuck water-in is connected with described heating in water bath case with chuck water outlet, and the temperature realizing water in described chuck controls.
Further, wrap at described substratum and be provided with respiratory siphon, described respiratory siphon arranges air filter.
Further, arrange peristaltic pump in described controller case, the pipe of described interchanger water-in is connected to described water source by described peristaltic pump.
Further, at the water inlet pipe of described substratum bag, rising pipe and breathing pipe end, all pipe clamp is set.
A kind of microbiological culture media compound method, comprises the steps:
(1) preparing tank and accessory thereof are carried out sterilising treatment;
(2) preparing tank, substratum bag are connected with controller case, the rising pipe of the feed-pipe of preparing tank with substratum bag is connected, the water inlet pipe of described substratum bag is connected with the interchanger water outlet of described controller case, the interchanger water-in of described controller case is connected with water source by pipe connecting, and described pipe connecting is arranged on the pump head of peristaltic pump;
(3) start peristaltic pump, the water at water source is entered controller case from the water-in of controller case and is heated by interchanger, discharged by water port simultaneously;
(4) after water temperature reaches preset temp, water port is closed automatically, and the water outlet of controller case is opened automatically, and heating water enters substratum bag;
(5) when the water yield injecting substratum bag reaches the volume of dissolution of setting, peristaltic pump stops;
(6) disposable injection preparing tank after the culture medium dry powder in substratum bag all dissolves or reaches mixing state;
(7) again start peristaltic pump, the hot water after being heated by interchanger is directly injected in preparing tank through described substratum bag, until the water yield in preparing tank reaches preset value;
(8), after having prepared, the feed-pipe of preparing tank and the pipe clamp of discharge nozzle is closed.
Further, described substratum bag is arranged on a vibrator, and when described substratum bag water filling is dissolved, described vibrator makes described substratum bag vibrate.
A kind of biological medium method for filling, comprises the steps:
(1) pump head of the peristaltic pump of the discharge nozzle via controller case of preparing tank is connected on packing vessel;
(2) described peristaltic pump is started, the cultivation base unit weight that the injection successively in each packing vessel is preset.
Further, also comprise the steps: to set dispensed loading amount, the time-out duration of peristaltic pump between packing two vessel in the quantity of packing vessel, each vessel between described (1) step and (2).
The invention has the beneficial effects as follows:
(1) preparing in substratum packet procedures, have employed radiation mode sterilising medium, and make disposable sterilized bag, only need during use to import preparing tank post-heating to agar melt temperature by after the molten water of dry powder in substratum bag, and preparing tank can in steam sterilizer sterilising treatment, owing to being slack tank, not containing substratum, temperature can also than higher during sterilising medium, and the time is longer, makes preparing tank sterilizing more thorough.Because radiation mode is very little (see Zhou Aiyi on medium component impact, Chen Huiyuan, Cai Zhihe, 60Co γ irradiation sterilization is on the impact of microbial culture product. Chinese Journal of Health Laboratory Technology, 2010,20 (12): 3558-3560) loss because of nutritive substance during high-temp steam sterilizing, is greatly reduced.
(2) adopt dehydrated medium bag, according to preparing tank use volume needed for cultivation base unit weight encapsulate, only it need be connected with the pipeline on preparing tank and controller during use.Therefore exempt the step of the time and effort consumings such as manual weighing, mixing, adjustment pH and fallibility, and add water, heat, the action such as start and stop peristaltic pump and magnetic motor is all automatically to have controlled.Therefore, human cost, time cost and product quality variance is greatly reduced.
(3) in whole substratum process for preparation, dehydrated medium dry powder is all in the substratum bag and preparing tank of sealing, does not have and extraneous contact, therefore produces without dust, avoids dust to the pollution of clean environment and the health endangering operator.
(4) product of the present invention can maintain substratum constant temp and the whipped state of melting state, and is furnished with peristaltic pump, and a set of sequence of control, therefore can realize the semi-automatic and manual packing function of substratum.
(5) dispenser structure is simple, easy to operate, and cost is low.
(6) the packing process visualization (in glass tank body situation) of substratum, can see material mixing situation in tank, have the residual content change of bubble-free, point process of assembling.
(7) dispenser can be supporting with substratum automatic separated loading instrument, also can independently use, and carry out semi-automatic or manual packing, separation container, dispensed loading amount are unrestricted.
Accompanying drawing explanation
Accompanying drawing 1 is the structure iron of microbiological culture media dispenser of the present invention;
Accompanying drawing 2 is structural representations of preparing tank;
Accompanying drawing 3 is the structural representations at a visual angle of controller case;
Accompanying drawing 4 is the structural representations at another visual angle of controller case;
Accompanying drawing 5 is controller case internal structure figures;
Accompanying drawing 6 is structural representations of substratum bag;
Pipeline connection diagram when accompanying drawing 7 is preparations;
Accompanying drawing 8 is programflow charts of compound method;
Accompanying drawing 9 is programflow charts of method for filling;
Pipeline connection diagram when accompanying drawing 10 is packing.
Label in accompanying drawing is respectively:
1. preparing tank; 11. blades;
12. agitators; 13. discharge nozzles;
14. electrode sheaths; 15. air filters;
16. material-feeding ports; 17. reserved opening;
18. culture medium solutions; 19. feed-pipes;
191. charging opening; 192. silicone tube;
2. controller case; 21. hot-plates;
22. peristaltic pumps; 23. liquid crystal displays;
24. wires; 25. temperature electrodes;
26. water port; 27. water-ins;
28. water outlets; 31. silicone tubes;
32. separatory mouths; 33. magnetic valves or pipe clamp;
41. general supply interfaces; 42. data transmission interfaces;
43. water source access ports; 44. water port;
45. entrance of cooling water; 46. cooling water outlets;
47. chuck water inlet interfaces; 48. chuck backwater interfaces;
51. times mouths of a river; 52. filling pipe ends;
6. substratum bag; 61. water inlet pipes;
611. sebific duct; 612. magnetic valves or pipe clamp;
613. strainer; 62. rising pipes;
621. magnetic valves or pipe clamp; 63. pneumostomes;
631. air filter; 632. magnetic valves or pipe clamp;
71. interchanger; 72. peristaltic pump drive-motor;
73. heating in water bath casees; 74. magnetic agitation motors;
75. heating components; 76. circuit cards;
81. Distilled water buckets; 82. silicone tubes;
91. vessel.
Embodiment
Elaborate below in conjunction with the embodiment of accompanying drawing to microbiological culture media dispenser of the present invention, microbiological culture media compound method and method for filling.
See accompanying drawing 1,2, microbiological culture media dispenser has and stirs and Temperature Measure Control function, and agitating function is used for accelerating substratum dissolving and the mixing state of maintain base; Temperature control system is used for realizing substratum and dissolves, melt and divide the temperature requirement in process of assembling.
Microbiological culture media dispenser comprises preparing tank, substratum bag and controller case, and substratum preparing tank 1, as the container of preparation culture medium solution 18, can be the glass pot of a jacketed or stainless cylinder of steel, or the individual layer tank body of not jacketed.Also can be the tank of other materials or shape, bucket or bottle etc.Chuck can be only side-wall jacket or only bottom chuck, or sidewall and bottom are jacket structured.Chuck has filling pipe end 52 and the lower mouth of a river 51.Preparing tank top has multiple perforate or conduit to insert, and is the pneumostome of feed-pipe 19, discharge nozzle 13, thermometer protecting pipe 14, charging opening 191, material-feeding port 16, reserved opening 17, connection air filter 15 respectively.Whole substratum preparing tank and above accessories all can high-temp steam sterilizings.Silicone tube 31 and 192 can be connected to discharge nozzle 13 sterilizing together with on feed-pipe 19 during sterilizing, silicone tube 31 outlet connects a separatory mouth 32.Silicone tube 192 is provided with pipe clamp 621, is provided with agitator 12 in preparing tank 1, is blade 11 below agitator 12.
See accompanying drawing 1,3,4, controller case 2 is used to set, monitor and forecast preparation substratum time required various parameters, and perform corresponding operation, as substratum temperature, mixing speed, add volume of water etc.The water outlet of described controller case is connected to the water inlet pipe of described substratum bag, and the rising pipe of described substratum bag is connected to the feed-pipe of described preparing tank.The control module of controller case 2 completes above-mentioned software function.
See accompanying drawing 4,5, the operating panel of controller is provided with peristaltic pump 22, interchanger water-in 27, interchanger water outlet 28, interchanger water port 26 and liquid crystal display 23; The rear panel of controller there are the water inlet of general supply interface 41, data transmission interface 42, water source access port 43, water port 44, chuck interface 47, chuck backwater interface 48, entrance of cooling water 45, cooling water outlet 46; Interchanger 71, peristaltic pump drive-motor 72, heating in water bath case 73, magnetic agitation motor 74, heating component 75, circuit card 76 and the wire be connected is had in controller case.Magnetic agitation motor 74 stirs device 12 for driving, and interchanger 71 is for heating into tank water, and heating in water bath case 73 is for the water heated or freeze in preparing tank chuck, and heating component 75 is used for as individual layer preparing tank provides transmission of heat by contact.
See accompanying drawing 6, according to the use volume of preparing tank 1, pre-install the dehydrated medium amount needed for respective volume at substratum bag 6, and in advance through irradiation sterilization.Substratum bag can select batch cultur Ji Bao, for subsequent use after prior sterilizing.Substratum wraps the respiratory siphon 63 of the water inlet pipe 61 be connected with controller box upper water-out mouth 28, the rising pipe 62 be connected with preparing tank top material inlet pipe 19 and maintain base bag internal and external pressure balance.Water inlet pipe 61 connects on water inflow filter 613, respiratory siphon 63 and connect air filter 631, respiratory siphon 63 is arranged pipe clamp 632, connection rubber tube 611 on water inlet pipe 61, sebific duct 611 is also provided with pipe clamp 612.
See accompanying drawing 7, pipeline during preparation connect and use procedure as follows: first preparing tank and some accessories are put into Sterilizers sterilizing, need the accessory of sterilizing to comprise: the flexible pipe 31 be connected with preparing tank top discharge pipe 13 and the separatory mouth 32 of end, the flexible pipe 192 be connected with feed-pipe 19.Flexible pipe 31 is provided with pipe clamp 33, if water inflow filter 613 is that to constitute substratum bag as the part of substratum bag overall, then water inflow filter 613 irradiation sterilization, otherwise, then need for subsequent use for water inflow filter 613 sterilizing together with preparing tank.
Under aseptic technique, be connected by the preparing tank after sterilizing with substratum bag, the rising pipe 62 wrapped by substratum is connected with flexible pipe 192.Water inflow filter 613 after sterilizing and substratum are wrapped water inlet pipe 61 and be connected (if water inflow filter 613 provides with substratum bag, then this step attended operation does not need).
Be placed in by preparing tank above controller box, be connected on water inflow filter 613 by one of water inlet pipe 611, other end is connected on the water outlet 28 on controller box front panel; Be connected on the water-in 27 on front panel by one of a silicone tube 82, other end is connected on distilled water or deionized water water source, as Distilled water bucket 81; Silicone tube 82 is clamped on Peristaltic pump head; Temperature electrode 25 is inserted the electrode sheath 14 li of preparing tank; Temperature signal is sent to control module by the wire 24 of temperature electrode 25.Interface 47 of being intake with the chuck on controller box rear panel respectively in the filling pipe end 52 of preparing tank chuck, the lower mouth of a river 51 is connected with chuck backwater interface 48.
See accompanying drawing 8, the significant parameter of substratum preparation arranges as follows: flow process display set temperature, rotating speed and volumetric parameter, specifically comprise: enter tank water temperature, to meet the optimal temperature demand that medium component dissolves; Agar melt temperature, when entering after tank culture medium solution reaches certain volume to be rapidly heated, so that fast melt agar composition; Moisturizing temperature, temperature in tank lowers, and keeps substratum to be melting state after melting by agar as early as possible; Temperature during packing, after substratum preparation procedure terminates, temperature when waiting to be packed.Peristaltic pump rotating speed; Magnetic motor rotating speed, meets the stirring requirement of process for preparation different steps.Volume of dissolution (the volume of≤substratum bag, be beneficial to first substratum bag substratum be dissolved, and conveniently import to preparing tank), melt volume (be about preparation cumulative volume about 20%, small volume is convenient to the thawing agar that is rapidly heated, thawing is quick to be afterwards reduced to close to more than packing temperature a certain temperature by temperature, reduce high temperature to the impact of medium component) and preparation cumulative volume.
By dispenser of the present invention, realize microbiological culture media preparation, its method comprises the steps:
(1) preparing tank and accessory thereof are carried out sterilising treatment;
(2) preparing tank, substratum bag are connected with controller case, the rising pipe of the feed-pipe of preparing tank with substratum bag is connected, the water inlet pipe of described substratum bag is connected with the water outlet of described controller case, the water-in of described controller case is connected with water source by pipe connecting, and described pipe connecting is arranged on the pump head of described peristaltic pump;
(3) start peristaltic pump, the water at water source is entered controller case from the water-in of controller case and is heated by interchanger, discharged by water port simultaneously;
(4) after water temperature reaches preset temp, water port is closed automatically, and the water outlet of controller case is opened automatically, and heating water enters substratum bag;
(5) when the water yield injecting substratum bag reaches the volume of dissolution of setting, peristaltic pump stops;
(6) disposable injection preparing tank after the substratum in substratum bag all dissolves or reaches mixing state;
(7) again start peristaltic pump, the hot water after being heated by interchanger is directly injected in preparing tank through described substratum bag, until the water yield in preparing tank reaches preset value;
(8), after having prepared, the feed-pipe of preparing tank and the pipe clamp of discharge nozzle is closed.
Detailed process is as follows: after startup, peristaltic pump entry into service, starts to pump into distilled water, and when distilled water flows through interchanger 71, water is heated, and when water temperature does not reach the substratum solvent temperature of setting, water is discharged from water port 26, can directly discharge or be back to water butt; When water temperature reaches after tank water temperature, water port 26 is closed automatically, and water flows out from water outlet 28,611 flows to substratum bag 6 by the road.After the water yield pumped into reaches the volume of dissolution of setting, peristaltic pump automatic pause.In this stage, along with current enter substratum bag, substratum starts to dissolve, and completely after mixing, disposablely imports to preparing tank until substratum in water.If wish that whole process for preparation is unmanned intervention completely, then substratum bag can be placed on a vibrator, vibrator can place the position higher than tank deck water inlet pipe 19, and vibrator is controlled by console controller, accelerates substratum dissolve and mixing by vibration.When the magnetic valve 621 on pipe connecting 192 is opened, the substratum after dissolving flows into preparing tank.Another kind of mode adopts and manually rocks substratum bag, even both hands rubbing substratum bag, accelerate dissolution.After substratum dissolves, unclamp pipe clamp 621, open pipe clamp 632, utilize the air pressure inside and outside pneumostome 63 equilibrated Medium bag, by disposable for culture medium solution importing preparing tank.
After culture medium solution imports preparing tank, again start the peristaltic pump of time-out, continue to add water in tank through substratum bag, and rinse culture medium solution residual in substratum bag, or increase once intermittent flushing operation, reduce medium component residual in substratum bag as far as possible.
Once interchanger 71 starts heating schedule, then start the heating schedule of heating in water bath case 73 simultaneously, after the water temperature in heating in water bath case 73 reaches set(ting)value, maintain this temperature and start chuck circulation.After the accumulative water yield pumped in tank reaches and melts volume (be about about 20% that culture volume is always prepared in budget), peristaltic pump automatic pause, magnetic agitation motor starts automatically, and run under the rotating speed preset, and the heating in water bath case 73 flowing through jacket water (J.W.) starts heating schedule automatically, rise to temperature in tank always and reach agar melt temperature, and maintain several minutes (can be determined by test, so that agar melts completely).Automatically reduce chuck water temperature afterwards, with maintain substratum temperature in tank setting moisturizing temperature value (about 60 DEG C, can be determined by test, keep agar in tank and packing time be in melting state always).When temperature in tank reaches after predetermined holding time in agar melt temperature value, peristaltic pump is autoboot again, and starts the alternating temperature program of interchanger simultaneously, to keep moisturizing temperature consistent with holding temperature in tank (about 60 DEG C).If preparing tank is the individual layer tank body not having chuck, then the hot-plate 21 of controller upper central position can be adopted to carry out substratum in heating tank.
When after the preparation cumulative volume that the distilled water pumping into preparing tank reaches default, peristaltic pump shuts down.If in tank, temperature is higher than the temperature required during substratum packing during moisturizing, then heating in water bath case starts cooling process automatically, the temperature needed during to reach packing.If adopt hot-plate 21 mode to heat preparing tank, then by regulating hot-plate to change temperature in tank.
When reaching predetermined preparation cumulative volume in preparing tank and when in tank, temperature reaches packing holding temperature, controller all can send information, and pipe clamp 621 and 612 can clamp by operator, or adopts magnetic valve to substitute pipe clamp, automatically closes.The temperature needed during equipment maintenance packing and rotating speed, until packaging operation terminates.
Equipment can carry out parameter modification and confirmation when running at any time, performs new operational requirement.
The timing node of the heating module start and stop of process for preparation and temperature value, peristaltic pump start-stop time node and rotating speed and accumulation at that time at that time add the information such as the volume of distilled water and all can go on record.
Substratum packing completes as follows:
(1) discharge nozzle of preparing tank is connected on the peristaltic pump of controller case;
(2) by the dispensed loading amount in the quantity of program setting packing vessel, each vessel, the time-out duration of peristaltic pump between packing two vessel;
(3) described peristaltic pump is started, the cultivation base unit weight that the injection successively in each packing vessel is preset.
If user has automatic substratum packing instrument, the discharge nozzle of substratum preparing tank can be directly connected on automatic substratum packing instrument, by packing time variable control and the packing of automatic substratum packing instrument.
See accompanying drawing 9,10, design parameter configures and divides process of assembling as follows: the substratum prepared can perform multiple packing mode:
1. the substratum automatic separated loading instrument with other is supporting, by automatic separated loading instrument control packaging operation;
2. the packing program adopting present device to carry carries out semi-automatic or manual packing.
When adopting the packing program carried, the dull and stereotyped or pipe of each vessel 91(of setting or bottle before packing) in dispensed loading amount, the rotating speed of pump.
If there is special composition to need to add separately, then added from the material-feeding port of tank top by syringe;
Also can select semi-automatic packing or manual packing, under semi-automatic packing pattern, need to set the time-out duration of peristaltic pump between packing two vessel 91 and vessel 91 quantity of every component dress.It is that point pump installed before vessel 91 to the next vessel 91 of packing suspends duration that two vessel 91 suspend duration, and interval manually replaces vessel 91 to be packed for operator;
Due to non-full automatically subpackaging, there is no the automatic buttress dish function of culture dish, vessel 91 limited amount of operating table surface last energy packing, so need vessel 91 quantity of a setting packing, after reaching this quantity, peristaltic pump automatic pause, vessel 91 quantity that every component that Here it is fills.Continue the packing of next group vessel 91, need again to click start button, perform vessel 91 packing of next group setting quantity;
Under manual mode, can every packing vessel 91, point clicks button, can by the volume packing preset.If long by not putting, then peristaltic pump rotates continuously.
Under semiautomatic-mode, the dispensed loading amount of each vessel 91, accumulation packing vessel 91 quantity, point ETL estimated time of loading, end time, substratum title, operation are automatically recorded per capita.
Below be only the preferred embodiment of the present invention, it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention, can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (8)
1. a microbiological culture media dispenser, it is characterized in that: comprise preparing tank, substratum bag and controller case, described preparing tank is provided with feed-pipe, discharge nozzle, described substratum is wrapped and is provided with water inlet pipe and rising pipe, the water inlet pipe of described substratum bag connects water source, the feed-water end of described substratum bag is provided with water inflow filter, the rising pipe of described substratum bag is connected to the feed-pipe of described preparing tank, heating unit and control unit is provided with in described controller case, described heating unit heats described preparing tank, temperature sensor is provided with in described preparing tank, described temperature sensor is connected to described control unit, described control unit adjusts the temperature of culture medium solution in described preparing tank in real time according to the feedback of described temperature sensor, in described preparing tank, agitator is set, described agitator is controlled to stir the culture medium solution in described preparing tank by the control unit of described controller case.
2. microbiological culture media dispenser according to claim 1, it is characterized in that: described controller case comprises interchanger, interchanger water-in, interchanger water outlet and water port, the interchanger water-in of described controller case is connected to described water source, the interchanger water outlet of described controller case is connected to the water inlet pipe of described substratum bag, and described control unit controls the water outlet of described interchanger water outlet or described water port draining according to the output water temperature of described interchanger.
3. microbiological culture media dispenser according to claim 1, it is characterized in that: described preparing tank is provided with chuck, at the sidewall of the chuck of described preparing tank, chuck water-in and chuck water outlet are set, in described controller case, also arrange heating in water bath case, described chuck water-in is connected with described heating in water bath case the temperature realizing water in described chuck with chuck water outlet and controls.
4. microbiological culture media dispenser according to any one of claim 1 to 3, is characterized in that: wrap at described substratum and be provided with respiratory siphon, described respiratory siphon arranges air filter.
5. microbiological culture media dispenser according to claim 2, is characterized in that: arrange peristaltic pump in described controller case, the pipe of described interchanger water-in is connected to described water source by described peristaltic pump.
6. microbiological culture media dispenser according to claim 4, is characterized in that: all arrange pipe clamp at the water inlet pipe of described substratum bag, rising pipe and breathing pipe end.
7. a microbiological culture media compound method, uses dispenser as claimed in claim 4, it is characterized in that: comprise the steps:
(1) preparing tank and accessory thereof are carried out sterilising treatment;
(2) preparing tank, substratum bag are connected with controller case, the rising pipe of the feed-pipe of preparing tank with substratum bag is connected, the water inlet pipe of described substratum bag is connected with the interchanger water outlet of described controller case, the interchanger water-in of described controller case is connected with water source by pipe connecting, and described pipe connecting is arranged on the pump head of peristaltic pump;
(3) start peristaltic pump, the water at water source is entered controller case from the water-in of controller case and is heated by interchanger, discharged by water port simultaneously;
(4) after water temperature reaches preset temp, water port is closed automatically, and the water outlet of controller case is opened automatically, and heating water enters substratum bag;
(5) when the water yield injecting substratum bag reaches the volume of dissolution of setting, peristaltic pump stops;
(6) disposable injection preparing tank after the culture medium dry powder in substratum bag all dissolves or reaches mixing state;
(7) again start peristaltic pump, the hot water after being heated by interchanger is directly injected in preparing tank through described substratum bag, until the water yield in preparing tank reaches preset value;
(8), after having prepared, the feed-pipe of preparing tank and the pipe clamp of discharge nozzle is closed.
8. microbiological culture media compound method according to claim 7, is characterized in that: described substratum bag is arranged on a vibrator, and when described substratum bag water filling is dissolved, described vibrator makes described substratum bag vibrate.
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