CN104472343B - A kind of selection of Wuta-tsai self incompatible line - Google Patents
A kind of selection of Wuta-tsai self incompatible line Download PDFInfo
- Publication number
- CN104472343B CN104472343B CN201410783841.4A CN201410783841A CN104472343B CN 104472343 B CN104472343 B CN 104472343B CN 201410783841 A CN201410783841 A CN 201410783841A CN 104472343 B CN104472343 B CN 104472343B
- Authority
- CN
- China
- Prior art keywords
- wuta
- tsai
- self
- incompatible line
- self incompatible
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000000306 recurrent effect Effects 0.000 claims abstract description 19
- 238000012216 screening Methods 0.000 claims abstract description 10
- 238000009331 sowing Methods 0.000 claims description 6
- 238000009396 hybridization Methods 0.000 claims description 5
- 238000000034 method Methods 0.000 abstract description 19
- 238000009395 breeding Methods 0.000 abstract description 12
- 239000000463 material Substances 0.000 abstract description 8
- 230000001488 breeding effect Effects 0.000 abstract description 4
- 201000004569 Blindness Diseases 0.000 abstract description 2
- 238000007796 conventional method Methods 0.000 abstract description 2
- 238000000926 separation method Methods 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 description 41
- 230000005849 recognition of pollen Effects 0.000 description 6
- 240000007124 Brassica oleracea Species 0.000 description 5
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 5
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 5
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 5
- 230000010152 pollination Effects 0.000 description 4
- PXFBZOLANLWPMH-UHFFFAOYSA-N 16-Epiaffinine Natural products C1C(C2=CC=CC=C2N2)=C2C(=O)CC2C(=CC)CN(C)C1C2CO PXFBZOLANLWPMH-UHFFFAOYSA-N 0.000 description 3
- 235000013311 vegetables Nutrition 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 244000060696 Alpinia speciosa Species 0.000 description 1
- 235000013411 Alpinia speciosa Nutrition 0.000 description 1
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 1
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 1
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 1
- 241000219193 Brassicaceae Species 0.000 description 1
- 238000000205 computational method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000012214 genetic breeding Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000009399 inbreeding Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses the selection of a kind of Wuta-tsai self incompatible line, step includes: using Wuta-tsai self compatible line as recurrent parent, using Xiaoqinling Nature Reserve self incompatible line as proceeding to source, filial generation and recurrent parent backcross after some generations, it is thus achieved that stable Wuta-tsai self incompatible line; The Xiaoqinling Nature Reserve that the method utilizes with Wuta-tsai sibship is extremely near and selfing not affinitive material is abundant proceeds to source as self incompatible line, in conjunction with selfing separation screening offspring, reach the purpose of selection-breeding Wuta-tsai self incompatible line efficient, quick, more conventional method greatly reduces the blindness of work, time saving and energy saving, parent material wide material sources simultaneously, overcome the technical problem that Wuta-tsai self incompatible line breed breeding is difficult.
Description
Technical field
The present invention relates to the technical field of a kind of Crop Genetic Breeding, in particular the selection of a kind of Wuta-tsai self incompatible line.
Background technology
The approach obtaining self incompatible line at present on brassicaceous vegetable mainly has following 2 kinds: 1) selfing partition method (Du Min, the selection-breeding of brassicaceous vegetable self incompatible line and utilization, China seed industry, 2005), namely adopting florescence selfing and the not affine strain of flower bud phase selfed breeding selfing, then use pollen mixture pollination method selection-breeding self incompatible line in offspring, this method workload is big, cycle is long, and efficiency is low; 2) interbreed method in planting, such as Ma Chaozhi, Fu Tingdong, " selection of a kind of cabbage type rape self-incompatibility two-line cenospecies " that Gao Changbin et al. provides, the method is with cabbage type rape of self incompatible lin S-1300 for female parent, No. 3 hybridization double; two with self-compatible cabbage type rape strain China, obtain F1 seed; Utilize the self-compatibility of index of affinity method detection F1 plant, the incompatible plant of F1 is carried out stripping flower bud selfing, obtain F2 plant, the affinity of detection F2 plant, select not affine plant to carry out manually stripping flower bud selfing, obtain F3 seed; Sowing F3 seed, selects F3 family, and to all individual plant bagging selfings choosing strain, each strain selects 3��5 individual plants to carry out manually stripping flower bud selfing, obtains the seed of F4; Sowing individual plant index of affinity is respectively less than in the F3 family of 1 seed of results, select F4 family, to choosing all individual plant bagging selfings of strain, each strain selects 3��5 individual plants manually to shell flower bud selfing, and selecting the F4 family that index of affinity is respectively less than 1 is required cabbage type rape of self incompatible lin; The F3 family that self-incompatibility is unstable, repeats above-mentioned selection-breeding step, until selecting the cabbage type rape of self incompatible lin that self-incompatibility is stable; This method is to create and the transformation simplest method of self-incompatibility material, but the genetic resources being as in the single kind of plant is day by day exhausted, and the method is limited between kind, self-incompatible proceeds to narrow range.
Wuta-tsai is a mutation of Cruciferae Btassica Semen Brassicae Campestris kind Chinese cabbage subspecies, up to now, not yet it is reported that find the not affine plant of selfing of Wuta-tsai in experiment in cultivation, regardless of whether interbreed method in utilizing selfing partition method or planting, all it is difficult to obtain Wuta-tsai self incompatible line kind.
Summary of the invention
It is an object of the invention to overcome the deficiencies in the prior art, it is provided that the selection of a kind of Wuta-tsai self incompatible line, the technical problem difficult to solve Wuta-tsai self incompatible line breed breeding.
The present invention is achieved by the following technical solutions:
The selection of a kind of Wuta-tsai self incompatible line, comprises the following steps:
(1) using Wuta-tsai self compatible line as recurrent parent, using Xiaoqinling Nature Reserve self incompatible line as proceeding to source, hybridization obtains F1 seed;
(2) the F1 seed of sowing step (1), obtain F1 plant, F1 plant is carried out florescence selfing and the selfing of flower bud phase, measure its index of affinity, select the index of affinity of florescence selfing less than 1 and the index of affinity of flower bud phase selfing more than 10 and self-incompatible F1 plant that character is consistent with recurrent parent, backcross with recurrent parent, it is thus achieved that F2 seed;
(3) offspring repeats the screening of step (2) and back-crossing step n-2 time, it is thus achieved that Fn plant, when the florescence index of affinity of all individual plants of described Fn plant is less than 1, when flower bud phase index of affinity is more than 10, is stable Wuta-tsai self incompatible line.
The Wuta-tsai self compatible line of described step (1) one in S9701, W9703, Huainan evil mind crow and Hefei evil mind crow.
The Xiaoqinling Nature Reserve self incompatible line of described step (1) one in Xiaoqinling Nature Reserve Hua Guan, summer king, Xia Guanhe summer anti-self incompatible line.
The n of described step (3) is equal to 4 or 5.
The present invention has the advantage that the selection that the invention provides a kind of Wuta-tsai self incompatible line compared to existing technology, the method is on the basis of the approach of existing two kinds of brassicaceous vegetable selection-breeding self incompatible lines, by breaking kind of a restriction for interior interbreed, source is proceeded to as self incompatible line with the abundant Xiaoqinling Nature Reserve of and selfing not affinitive material extremely near with Wuta-tsai sibship, reach efficiently in combination with selfing separation screening offspring, the purpose of quick selection-breeding Wuta-tsai self incompatible line, more conventional method greatly reduces the blindness of work, time saving and energy saving, parent material wide material sources simultaneously, overcome the technical problem that Wuta-tsai self incompatible line breed breeding is difficult.
Accompanying drawing explanation
Fig. 1 is the selection-breeding flow chart of self incompatible line.
Detailed description of the invention
Below in conjunction with accompanying drawing, embodiments of the invention being elaborated, the present embodiment is carried out under premised on technical solution of the present invention, gives detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
Embodiment 1
The present embodiment is the selection of Wuta-tsai S9701 self incompatible line, and flow chart as shown in Figure 1, comprises the following steps:
1, material prepares:
Wuta-tsai S9701 self compatible line: S9701 is through many excellent self compatible line kinds for isolation and selection by the Wuta-tsai kind in Huainan.
Xiaoqinling Nature Reserve China crown-5A self incompatible line: the China's hat kind introduced by Japan, separates the stable Xiaoqinling Nature Reserve self incompatible line kind obtained through inbreeding of more generation.
2, selection-breeding step:
(1) using Wuta-tsai S9701 self compatible line as recurrent parent, using Xiaoqinling Nature Reserve self incompatible line China crown-5A as proceeding to source, hybridization obtains F1 seed;
(2) the F1 seed of sowing step (1), obtain F1 plant, the F1 plant that screening character is consistent with recurrent parent, the main inflorescence of the F1 plant of all screenings is carried out bagging, carry out florescence selfing and the selfing of flower bud phase respectively, utilizing index of affinity method to measure its affinity, described index of affinity method measures the step of index of affinity and includes:
I, florescence selfing: by F1 plant inflorescence bagging, after alabastrum in bag is open, coats the pollen of the prior bagging of homophyletic, pollination front and back bagging all immediately on stigma, pass part with protection against insect;
II, the selfing of flower bud phase: by F1 plant inflorescence bagging, select 1��2 inflorescence to pollinate simultaneously on homophyletic, by the alabastrum tip tweezers strip off of 2��4 days before blooming, stigma is made to expose, coat the pollen of the prior bagging of homophyletic, pollination front and back bagging all immediately, pass part with protection against insect.
III, computational methods: number is spent in index of affinity=number/pollination of setting seeds
Select the index of affinity of florescence selfing less than 1 and the index of affinity of flower bud phase selfing more than 10 and self-incompatible F1 plant that character is consistent with recurrent parent, backcross with recurrent parent, obtain F2 seed;
(3) offspring repeats the screening of step (2) and back-crossing step 2 times, obtain F4 plant, F4 plant is carried out florescence selfing and the selfing of flower bud phase, index of affinity method is utilized to measure its affinity, result shows, the florescence index of affinity of all individual plants of F4 plant is less than 1, and the flower bud phase, index of affinity was more than 10, it is determined that the self-incompatibility shape in F4 plant is stably handed down by heredity.
Embodiment 2
The present embodiment is the selection of Hefei evil mind crow self incompatible line, and flow chart as shown in Figure 1, comprises the following steps:
(1) using Hefei evil mind crow self compatible line as recurrent parent, using Xiaoqinling Nature Reserve self incompatible line China crown-5A as proceeding to source, hybridization obtains F1 seed;
(2) the F1 seed of sowing step (1), obtain F1 plant, the F1 plant that screening character is consistent with recurrent parent, the main inflorescence of the F1 plant of all screenings is carried out bagging, carry out florescence selfing and the selfing of flower bud phase respectively, utilize index of affinity method to measure its affinity, select the index of affinity of florescence selfing less than 1 and the index of affinity of flower bud phase selfing more than 10 and self-incompatible F1 plant that character is consistent with recurrent parent, backcross with recurrent parent, obtain F2 seed;
(3) offspring repeats the screening of step (2) and back-crossing step 3 times, obtain F5 plant, F5 plant is carried out florescence selfing and the selfing of flower bud phase, index of affinity method is utilized to measure its affinity, result shows, the florescence index of affinity of all individual plants of F5 plant is less than 1, and the flower bud phase, index of affinity was more than 10, it is determined that the self-incompatibility shape in F5 plant is stably handed down by heredity.
Embodiment 3
The present embodiment is using Huainan evil mind crow self compatible line as recurrent parent, and using the self incompatible line of Xiaoqinling Nature Reserve summer king's kind as proceeding to source, other steps are with embodiment 1, it is thus achieved that F4 plant be stable Huainan evil mind crow self incompatible line.
Embodiment 4
The present embodiment, using Hefei evil mind crow self compatible line as recurrent parent, is preced with the self incompatible line of kind as proceeding to source using the Xiaoqinling Nature Reserve summer, and other steps are with embodiment 2, it is thus achieved that F5 plant be stable Hefei evil mind crow self incompatible line.
Embodiment 5
The present embodiment is using W9703 self compatible line as recurrent parent, and using the self incompatible line of Xiaoqinling Nature Reserve summer anti-kind as proceeding to source, other steps are with embodiment 1, it is thus achieved that F4 plant be stable W9703 self incompatible line.
Claims (4)
1. the selection of a Wuta-tsai self incompatible line, it is characterised in that comprise the following steps:
(1) using Wuta-tsai self compatible line as recurrent parent, using Xiaoqinling Nature Reserve self incompatible line as proceeding to source, hybridization obtains F1 seed;
(2) the F1 seed of sowing step (1), obtain F1 plant, F1 plant is carried out florescence selfing and the selfing of flower bud phase, measure its index of affinity, select the index of affinity of florescence selfing less than 1 and the index of affinity of flower bud phase selfing more than 10 and self-incompatible F1 plant that character is consistent with recurrent parent, backcross with recurrent parent, it is thus achieved that F2 seed;
(3) offspring repeats the screening of step (2) and back-crossing step n-2 time, it is thus achieved that Fn plant, when the florescence index of affinity of all individual plants of described Fn plant is less than 1, when flower bud phase index of affinity is more than 10, is stable Wuta-tsai self incompatible line.
2. the selection of a kind of Wuta-tsai self incompatible line according to claim 1, it is characterised in that the Wuta-tsai self compatible line of described step (1) one in Huainan evil mind crow and Hefei evil mind crow.
3. the selection of a kind of Wuta-tsai self incompatible line according to claim 1, it is characterised in that the Xiaoqinling Nature Reserve self incompatible line of described step (1) is the one in Xiaoqinling Nature Reserve Hua Guan and summer king's self incompatible line.
4. the selection of a kind of Wuta-tsai self incompatible line according to claim 1, it is characterised in that the n of described step (3) is equal to 4 or 5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410783841.4A CN104472343B (en) | 2014-12-17 | 2014-12-17 | A kind of selection of Wuta-tsai self incompatible line |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410783841.4A CN104472343B (en) | 2014-12-17 | 2014-12-17 | A kind of selection of Wuta-tsai self incompatible line |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104472343A CN104472343A (en) | 2015-04-01 |
| CN104472343B true CN104472343B (en) | 2016-06-08 |
Family
ID=52747069
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410783841.4A Active CN104472343B (en) | 2014-12-17 | 2014-12-17 | A kind of selection of Wuta-tsai self incompatible line |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104472343B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105815211A (en) * | 2016-03-26 | 2016-08-03 | 安徽科技学院 | Method for improving self-incompatibility Wuta-tsai hybridization efficiency |
| CN109566401B (en) * | 2019-01-14 | 2021-12-24 | 安徽农业大学 | Method for quickly breeding bolting-resistant homozygous inbred line and inbred-incompatible line of black cabbage pole |
-
2014
- 2014-12-17 CN CN201410783841.4A patent/CN104472343B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN104472343A (en) | 2015-04-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Khush et al. | Haploids in plant breeding | |
| CN106967726A (en) | It is a kind of to create Asian Cultivated Rice and the methods and applications of the affine system of Oryza glaberrima Steud interspecific hybrid | |
| CN104335889B (en) | The haploid method of inducing maize | |
| CN104472343B (en) | A kind of selection of Wuta-tsai self incompatible line | |
| CN103999769A (en) | Corn haploid inducing method | |
| CN104429918B (en) | A kind of method of Wild cabbage type recessive epistatic interaction core infertility rape temporary maintainer line improvement | |
| CN104026002B (en) | A kind of fast breeding method of series of three-series hybrid rice sterile line | |
| CN103733976B (en) | Utilize the method for the sterile cytoplasm seed selection male sterile line of bluish dogbane KN142 | |
| CN104521740B (en) | Synchronous transform-breeding method for wuta-cai homozygous inbred line and cytoplasmic male sterile line | |
| CN103329796A (en) | General method for rapidly and directionally cultivating novel rice cytoplasm male sterile restoration source | |
| CN103733978A (en) | Method for breeding male sterile line by use of sterile cytoplasm of hibiscus cannabinus KN250 | |
| CN103190335B (en) | Simple method for artificially synthesizing cabbage type rape | |
| CN103120118A (en) | Method for breeding cabbage type rape recessive epistasis interaction genic male sterility two type line | |
| CN1127893C (en) | Two-line male sterile method for assortively breeding ecological-type cytoplasm of rape | |
| CN101347095A (en) | Honglian type method for breeding japonica hybrid rice | |
| CN109362562A (en) | A kind of cabbage type rape Breeding for restoration lines method with orange suit markings character | |
| CN111406644B (en) | Cluster-growing pod pepper nuclear-cytoplasmic interaction type male sterile line breeding method and application of three-line matched system | |
| De Guzman et al. | Genetics and breeding system for cytoplasmic and genetic male sterility in rice | |
| CN107950381A (en) | Cabbage type rape yellow fraction spends the application of mutant | |
| CN105830914A (en) | Breeding method of cabbage-type rape high oil-content recessive epistatic interaction nuclear sterile homozygous two-type line | |
| CN104186302A (en) | Technical method for multi-genotype heterosis utilization by utilizing photoperiod non-sensitive genetic male-sterile line | |
| CN105638447A (en) | Breeding method for increasing rape nuclear sterile line yield and improving seed pureness | |
| CN107182778B (en) | A kind of affine screening technique for keeping system of " eastern wild type " series of three-series hybrid rice outcrossing | |
| CN105850715A (en) | Breeding method for cabbage-type rape high-oil-content recessive-epistatic-interaction nucleic-male-sterility homonuclear temporary maintaining line | |
| CN107372096A (en) | A kind of method of quick breeding nucleo-cytoplasmic interreaction sterile line |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20150401 Assignee: Ma'anshan Yuan Mei agricultural science and Technology Co., Ltd. Assignor: Anhui Agricultural University Contract record no.: 2018340000003 Denomination of invention: Breeding method for the self-incompatible line of brassica campestris L. ssp. chinensis (L.) makino var. rosularis tsen et lee Granted publication date: 20160608 License type: Exclusive License Record date: 20180419 |
|
| EE01 | Entry into force of recordation of patent licensing contract |