CN104435135A - Method of extracting non-alkaloid from fructus meliae toosendan and rhizoma corydalis - Google Patents
Method of extracting non-alkaloid from fructus meliae toosendan and rhizoma corydalis Download PDFInfo
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Abstract
The invention discloses a method of extracting non-alkaloid from fructus meliae toosendan and rhizoma corydalis, relates to an extraction method of the non-alkaloid and aims at solving the technical problems of the traditional method of extracting the non-alkaloid of low speed, high cost, need for reflux high temperature, complex operation, high cost of extraction process and low extraction efficiency. The method comprises the following steps: mixing the rhizoma corydalis and the fructus meliae toosendan, grinding, adding an ethanol aqueous solution, and carrying out ultrasonic treatment and microwave heating; then carrying out ultrasonic treatment, filtering, collecting a filtrate, steaming out ethanol in the filtrate, adding water to regulate a pH value, and collecting an ion exchange liquid containing the non-alkaloid component; and regulating a pH value to be 7, carrying out vacuum concentration, and drying under vacuum to prepare non-alkaloid powder. Compared with the decocting and alcohol precipitation processes, the method has the following characteristics of rapidness in extraction, high extraction rate, low cost, high efficiency, no need for reflux high temperature, simpleness and feasibility in operation, low cost of the extraction process and remarkable comprehensive economical benefit. The invention belongs to the field of extracting the non-alkaloid.
Description
Technical field
The present invention relates to a kind of extracting method of non-alkaloid.
Background technology
Sichuan Chinaberry Powder is the recipe of all pain cards for the treatment of trusted subordinate's gastral cavilty, and curative effect is better, and use safety, and Fructus Toosendan and Rhizoma Corydalis are the main two taste medicines of Chinese medicine Sichuan Chinaberry Powder.Research shows that the non-alkaloid components that Sichuan Chinaberry Powder extracts plays a major role on treatment gastric ulcer.The method of usual extraction non-alkaloid is decocting, then adds ethanol extraction, and the method operating process complexity, length consuming time, extraction efficiency extraction ratio that is low, non-alkaloid are also lower, are unfavorable for Study on Modernization and the application of Effective Component of Chinese Medicine.In ultrasound wave and microwave assisted extraction Chinese medicine, chemical composition is considered to a kind of method efficiently.But decocting in water, alcohol precipitation process are adopted at present for the extraction of non-alkaloid, but there is the problem that extraction rate is slow, the cycle is long, price is high and need reflux high temperature, complicated operation.
Summary of the invention
The object of the invention is that method extraction rate in order to solve existing extraction non-alkaloid is slow, the cycle is long, price is high and the high temperature that need reflux, complicated operation, the technical problem that extraction process cost is high, provides a kind of method extracting non-alkaloid from Fructus Toosendan and Rhizoma Corydalis.
The method extracting non-alkaloid from Fructus Toosendan and Rhizoma Corydalis is carried out according to following steps:
One, the Fructus Toosendan of the Rhizoma Corydalis of 100g and 200g is mixed, then grind, add ethanol water, obtain mixture;
Two, the mixture 10-20 minute that obtains of ultrasound treatment step one, temperature controls at 20-40 DEG C, ultrasonic power 100-200W, and ultrasonic frequency is 40KHz; Then transfer to microwave heating 10-20 minute in microwave reactor, microwave power is 260-520W, and frequency is 2450MHz; Again 20-40 DEG C, ultrasonic power is 100-200W, ultrasonic frequency be the condition of 40KHz under supersound process 10-20 minute, filter, collect filtrate, steam the ethanol in filtrate, add water to 200mL again, the pH value adding hydrochloric acid conditioning solution is 1-2, then crosses 731 type cation exchange resin columns, collects the ion exchange liquid containing non-alkaloid components;
Three, the pH value of the ion exchange liquid containing non-alkaloid components is adjusted, concentrating under reduced pressure, then dry under vacuo, obtained non-alkaloid powder.
In step one, ethanol water volume parts is 60-80%.
The inventive method is adopted to obtain the 6-9% that abiotic alkali number is raw materials quality, namely every gram of Rhizoma Corydalis and Fructus Toosendan can extract the non-alkaloid 60-90 milligram (method of decocting alcohol extraction, the most multipotency of every gram of this Rhizoma Corydalis and Fructus Toosendan mixture extracts non-alkaloid 15 milligrams, and needs repeatedly to extract for a long time).
This method adopts first supersound process, effectively can destroy rapidly Fructus Toosendan and the hard cell wall of Rhizoma Corydalis, make alcoholic solution and biological components effect, reach and make extraction component hold diffluent object; Microwave is a kind of Sasser that can be rapidly heated, and next uses microwave treatment, and each component molecular can be made to discharge fast from Fructus Toosendan and Rhizoma Corydalis.But long-time microwave heating can destroy non-alkaloid component molecule, thus loses activity.But effectively can not extract non-alkaloid from Fructus Toosendan and Rhizoma Corydalis by means of only ultrasonic and microwave treatment, the abiotic alkali number obtained is very low.Thus, cause to prevent long-time microwave treatment destroying non-alkaloid component and activity thereof, this method is taked control the microwave time and reuse ultrasonic Treatment to mixture subsequently.Like this, method of the present invention can extract the non-alkaloid component in Fructus Toosendan and Rhizoma Corydalis quickly and efficiently.The method, compared with decocting in water, alcohol precipitation process, has following outstanding feature: extract quick, inexpensive, efficient; Without the need to the high temperature that refluxes; Operation is simple; Extraction process cost is low, and overall economic efficiency is remarkable; Particularly getting efficiency height will much larger than decocting in water, alcohol precipitating method, and extraction efficiency can reach the 4-6 doubly left and right of decocting in water, alcohol precipitating method.
Detailed description of the invention
Technical solution of the present invention is not limited to following cited detailed description of the invention, also comprises the combination in any between each detailed description of the invention.
Detailed description of the invention one: the method extracting non-alkaloid from Fructus Toosendan and Rhizoma Corydalis is carried out according to following steps:
One, the Fructus Toosendan of the Rhizoma Corydalis of 100g and 200g is mixed, then grind into 20-40 object powder, add and account for Rhizoma Corydalis and Fructus Toosendan gross mass 3-5 ethanol water doubly, obtain mixture;
Two, the mixture 10-20 minute that obtains of ultrasound treatment step one, temperature controls at 20-40 DEG C, ultrasonic power 100-200W, and ultrasonic frequency is 40KHz; Then transfer to microwave heating 10-20 minute in microwave reactor, microwave power is 260-520W, and frequency is 2450MHz; Again 20-40 DEG C, ultrasonic power is 100-200W, ultrasonic frequency be the condition of 40KHz under supersound process 10-20 minute, filter, collect filtrate, steam the ethanol in filtrate, add water to 200mL again, the pH value adding hydrochloric acid conditioning solution is 1-2, then crosses 731 type cation exchange resin columns, collects the ion exchange liquid containing non-alkaloid components;
Three, add NaOH adjust pH to 7 to containing in the ion exchange liquid of non-alkaloid components, obtain the neutral ion exchange liquid containing non-alkaloid components, be less than concentrating under reduced pressure at 60 DEG C, then dry under vacuo, obtained non-alkaloid powder.
Detailed description of the invention two: present embodiment and detailed description of the invention one are 60-80% unlike ethanol water volume fraction in step one.Other is identical with detailed description of the invention one.
Detailed description of the invention three: one of present embodiment and detailed description of the invention one or two are Rhizoma Corydalis and Fructus Toosendan gross mass 4 times unlike the addition of ethanol water in step one.Other is identical with one of detailed description of the invention one or two.
Detailed description of the invention four: present embodiment and one of detailed description of the invention one to three are unlike grinding into 30 object powder in step one.Other is identical with one of detailed description of the invention one to three.
Detailed description of the invention five: the mixture 11-19 minute that one of present embodiment and detailed description of the invention one to four obtain unlike ultrasound treatment step in step 2 one, temperature controls at 22-38 DEG C, ultrasonic power 120-180W, and ultrasonic frequency is 40KHz.Other is identical with one of detailed description of the invention one to four.
Detailed description of the invention six: the mixture 13-17 minute that one of present embodiment and detailed description of the invention one to five obtain unlike ultrasound treatment step in step 2 one, temperature controls at 25-35 DEG C, ultrasonic power 130-260W, and ultrasonic frequency is 40KHz.Other is identical with one of detailed description of the invention one to five.
Detailed description of the invention seven: the mixture that one of present embodiment and detailed description of the invention one to six obtain unlike ultrasound treatment step in step 2 one 15 minutes, temperature controls at 30 DEG C, ultrasonic power 200W, and ultrasonic frequency is 40KHz.Other is identical with one of detailed description of the invention one to six.
Detailed description of the invention eight: then one of present embodiment and detailed description of the invention one to seven unlike transferring in step 2 with microwave heating 11-18 minute in microwave reactor, and microwave power is 280-500W, and frequency is 2450MHz.Other is identical with one of detailed description of the invention one to seven.
Detailed description of the invention nine: then one of present embodiment and detailed description of the invention one to eight unlike transferring in step 2 with microwave heating 12-17 minute in microwave reactor, and microwave power is 300-450W, and frequency is 2450MHz.Other is identical with one of detailed description of the invention one to eight.
Detailed description of the invention ten: one of present embodiment and detailed description of the invention one to nine use microwave heating 15 minutes unlike then transferring in step 2 in microwave reactor, and microwave power is 400W, and frequency is 2450MHz.Other is identical with one of detailed description of the invention one to nine.
Adopt following experimental verification effect of the present invention:
Experiment one:
The method that decoction and alcohol sedimentation technique extracts non-alkaloid is carried out according to following steps:
One, the Fructus Toosendan of the Rhizoma Corydalis of 100g and 200g is mixed, then add the distilled water accounting for Rhizoma Corydalis and Fructus Toosendan gross mass 8 times and decoct 2h, collect decoction liquor, then add the distilled water decoction 2h accounting for Rhizoma Corydalis and Fructus Toosendan gross mass 6 times, merge decoction liquor and concentrate, obtaining the concentrated solution of 500mL;
Two, add in concentrated solution mass concentration be 95% ethanol to volume fraction of ethanol in mixed liquor be 75%, filter after leaving standstill 24h, then the ethanol in filtrate is steamed, add water to 500mL again, adding adjust ph in concentrated hydrochloric acid to solution is 1, then cross 731 type cation exchange resin columns, collect the ion exchange liquid containing non-alkaloid components, then to extremely neutral containing adding NaOH adjust pH in the ion exchange liquid of non-alkaloid components; Concentrating under reduced pressure at 50 DEG C, then drying obtains non-alkaloid powder under vacuo.
Adopt this method every gram Rhizoma Corydalis and Fructus Toosendan mixture can extract non-alkaloid 10-15 milligram.
Experiment two:
The method of ultrasonic extraction method non-alkaloid is carried out according to following steps:
The Fructus Toosendan of the Rhizoma Corydalis of 100g and 200g is mixed, then 20-40 object powder is ground into, adding the mass concentration accounting for Rhizoma Corydalis and Fructus Toosendan gross mass 3-5 times is the ethanol water of 80%, first 8-12 hour is soaked, then supersound process said mixture 10-20 minute is used, temperature controls at 40 degree, ultrasonic power 100-200W, and ultrasonic frequency is 40KHz; Cooling, filtration, then the ethanol in filtrate is steamed, add water to 500mL again, adding adjust ph in concentrated hydrochloric acid to solution is 1, then 731 type cation exchange resin columns are crossed, collect the ion exchange liquid containing non-alkaloid components, then to extremely neutral containing adding NaOH adjust pH in the ion exchange liquid of non-alkaloid components; Concentrating under reduced pressure at 50 DEG C, then drying obtains non-alkaloid powder under vacuo.
Adopt the method every gram Rhizoma Corydalis and Fructus Toosendan mixture can extract non-alkaloid 8-12 milligram.
Experiment three:
The method of multistage microwave amplifier non-alkaloid is carried out according to following steps:
The Fructus Toosendan of the Rhizoma Corydalis of 100g and 200g is mixed, then 20-40 object powder is ground into, adding the mass concentration accounting for Rhizoma Corydalis and Fructus Toosendan gross mass 3-5 times is the ethanol water of 60-80%, be placed in microwave reactor, use microwave heating 10-20 minute, microwave power is 260-520W, and frequency is 2450MHz.After said process completes, mixture filtered while hot, collect filtrate, then the ethanol in filtrate is steamed, add water to 200mL again, the pH value adding hydrochloric acid conditioning solution is 1-2, then crosses 731 type cation exchange resin columns, collect the ion exchange liquid containing non-alkaloid components, then add NaOH adjust pH to 7 to containing in the ion exchange liquid of non-alkaloid components; The ion exchange liquid containing non-alkaloid components of gained neutrality, is being less than concentrating under reduced pressure at 60 DEG C, and then drying obtains non-alkaloid powder under vacuo.
Every gram of Rhizoma Corydalis and Fructus Toosendan can extract non-alkaloid 8-15 milligram.
Experiment four:
The method that ultrasonic-microwave method extracts non-alkaloid is carried out according to following steps:
The Fructus Toosendan of the Rhizoma Corydalis of 100g and 200g is mixed, then 20-40 object powder is ground into, adding 6 mass concentrations accounting for Rhizoma Corydalis and Fructus Toosendan gross mass 3-5 times is the ethanol water of 0-80%, first supersound process said mixture 10-20 minute is used, temperature controls at 20-40 degree, ultrasonic power 100-200W, ultrasonic frequency is 40KHz; Then transfer to microwave heating 10-20 minute in microwave reactor, microwave power is 260-520W, and frequency is 2450MHz.After said process completes, mixture filtered while hot, collect filtrate, then the ethanol in filtrate is steamed, add water to 200mL again, the pH value adding hydrochloric acid conditioning solution is 1-2, then crosses 731 type cation exchange resin columns, collect the ion exchange liquid containing non-alkaloid components, then add NaOH adjust pH to 7 to containing in the ion exchange liquid of non-alkaloid components; The ion exchange liquid containing non-alkaloid components of gained neutrality, is being less than concentrating under reduced pressure at 60 DEG C, and then drying obtains non-alkaloid powder under vacuo.
Adopt the method, every gram of Rhizoma Corydalis and Fructus Toosendan can extract non-alkaloid 22-28 milligram.
Experiment five:
The method that microwave-ultrasonic method extracts non-alkaloid is carried out according to following steps:
The Fructus Toosendan of the Rhizoma Corydalis of 100g and 200g is mixed, then 20-40 object powder is ground into, adding the mass concentration accounting for Rhizoma Corydalis and Fructus Toosendan gross mass 3-5 times is the ethanol water of 60-80%, first microwave heating 10-20 minute is used, microwave power is 260-520W, and frequency is 2450MHz.Then use supersound process said mixture 10-20 minute, temperature controls at 40 degree, ultrasonic power 100-200W, and ultrasonic frequency is 40KHz; Cooling, filtration, then the ethanol in filtrate is steamed, add water to 500mL again, adding adjust ph in concentrated hydrochloric acid to solution is 1, then 731 type cation exchange resin columns are crossed, collect the ion exchange liquid containing non-alkaloid components, then to extremely neutral containing adding NaOH adjust pH in the ion exchange liquid of non-alkaloid components; Concentrating under reduced pressure at 50 DEG C, then drying obtains non-alkaloid powder under vacuo.
Adopt the method every gram Rhizoma Corydalis and Fructus Toosendan mixture can extract non-alkaloid 15-21 milligram.
Experiment six:
The method (ultrasonic-microwave-ultrasonic method) of non-alkaloid is extracted from Fructus Toosendan and Rhizoma Corydalis:
The Fructus Toosendan of the Rhizoma Corydalis of 100g and 200g is mixed, then 30 object powder are ground into, adding the mass concentration accounting for Rhizoma Corydalis and Fructus Toosendan gross mass 5 times is the ethanol water of 80%, first supersound process said mixture 20 minutes are used, temperature controls at 40 DEG C, ultrasonic power 200W, ultrasonic frequency is 40KHz; Then transfer in microwave reactor and use microwave heating 15 minutes, microwave power is 390W, and frequency is 2450MHz; Finally, then said mixture is placed in ultrasonic instrument again, temperature be 40 DEG C, ultrasonic power 200W, ultrasonic frequency be the ultrasound condition of 40KHz under supersound process 20 minutes, filter, collect filtrate, steam the ethanol in filtrate, add water to 200mL again, the pH value adding hydrochloric acid conditioning solution is 1, then crosses 731 type cation exchange resin columns, collects the ion exchange liquid containing non-alkaloid components; Add NaOH adjust pH to 7 to containing in the ion exchange liquid of non-alkaloid components, obtain the neutral ion exchange liquid containing non-alkaloid components, be less than concentrating under reduced pressure at 60 DEG C, then dry under vacuo, obtained non-alkaloid powder.
This method obtain that abiotic alkali number is raw materials quality 9%, namely every gram of Rhizoma Corydalis and Fructus Toosendan can extract non-alkaloid 90 milligrams.
Experiment seven:
The method (ultrasonic-microwave-ultrasonic method) of non-alkaloid is extracted from Fructus Toosendan and Rhizoma Corydalis:
The Fructus Toosendan of the Rhizoma Corydalis of 100g and 200g is mixed, then 20 object powder are ground into, adding the mass concentration accounting for Rhizoma Corydalis and Fructus Toosendan gross mass 3 times is the ethanol water of 60%, first supersound process said mixture 10 minutes are used, temperature controls at 40 DEG C, ultrasonic power 100W, ultrasonic frequency is 40KHz; Then transfer in microwave reactor and use microwave heating 10 minutes, microwave power is 260W, and frequency is 2450MHz; Then said mixture is placed in ultrasonic instrument again, control at 40 DEG C in temperature, ultrasonic power 100W, ultrasonic frequency is supersound process 10 minutes under the ultrasound condition of 40KHz, filters, collect filtrate, steam the ethanol in filtrate, then add water to 200mL, the pH value adding hydrochloric acid conditioning solution is 1, then cross 731 type cation exchange resin columns, collect the ion exchange liquid containing non-alkaloid components; Add NaOH adjust pH to 7 to containing in the ion exchange liquid of non-alkaloid components, obtain the neutral ion exchange liquid containing non-alkaloid components, be less than concentrating under reduced pressure at 60 DEG C, then dry under vacuo, obtained non-alkaloid powder.
This experimental technique obtain that abiotic alkali number is raw materials quality 6%, namely every gram of Rhizoma Corydalis and Fructus Toosendan can extract non-alkaloid 60 milligrams.
Experiment eight:
The method (ultrasonic-microwave-ultrasonic method) of non-alkaloid is extracted from Fructus Toosendan and Rhizoma Corydalis:
The Fructus Toosendan of the Rhizoma Corydalis of 100g and 200g is mixed, then 40 object powder are ground into, adding the mass concentration accounting for Rhizoma Corydalis and Fructus Toosendan gross mass 4 times is the ethanol water of 70%, first supersound process said mixture 15 minutes are used, temperature controls at 30 DEG C, ultrasonic power 150W, ultrasonic frequency is 40KHz; Then transfer in microwave reactor and use microwave heating 15 minutes, microwave power is 312W, and frequency is 2450MHz; Finally, then said mixture is placed in ultrasonic instrument again, controls at 30 DEG C in temperature, ultrasonic power 150W, ultrasonic frequency is supersound process 15 minutes under 40KHz condition, filter, collect filtrate, steam the ethanol in filtrate, add water to 200mL again, the pH value adding hydrochloric acid conditioning solution is 1, then crosses 731 type cation exchange resin columns, collects the ion exchange liquid containing non-alkaloid components; Add NaOH adjust pH to 7 to containing in the ion exchange liquid of non-alkaloid components, obtain the neutral ion exchange liquid containing non-alkaloid components, be less than concentrating under reduced pressure at 60 DEG C, then dry under vacuo, obtained non-alkaloid powder.This experimental technique obtain that abiotic alkali number is raw materials quality 6.7%, namely every gram of Rhizoma Corydalis and Fructus Toosendan can extract non-alkaloid 78 milligrams.
Above Different Extraction Method is adopted from Rhizoma Corydalis and Fructus Toosendan, to extract the difference of the amount of non-alkaloid in table 1.
In sum, the water decoction-alcohol sedimentation method of current employing can only extract non-alkaloid 10-15mg from every gram of Rhizoma Corydalis and Fructus Toosendan, we attempt the method adopting independent ultrasound wave or microwave-assisted, can reach the extraction effect with water decoction-alcohol sedimentation method, non-alkaloid extracted amount is at most also at about 15mg/g; But can reach and shorten extraction time, make extraction process become simple; On this basis, carry out the ultrasonic non-alkaloid extracted with microwave combination in Rhizoma Corydalis and Fructus Toosendan, i.e. first ultrasonic method (microwave-ultrasonic method) after ultrasonic rear microwave method (Ultrasound-microwave) and first microwave, these two kinds of methods can improve the extracted amount of non-alkaloid further, and Ultrasound-microwave is better than microwave-ultrasonic method on non-alkaloid extracted amount, this is relevant with the ultrasonic cell wall that effectively can destroy Rhizoma Corydalis and Fructus Toosendan.But that the extracted amount of non-alkaloid increases and not obvious, experiment shows effectively to extract non-alkaloid further by extending microwave heating time, but for a long time microwave heating can destroy component and the activity of some non-alkaloids.Therefore, in order to improve the extracted amount of non-alkaloid in Rhizoma Corydalis and Fructus Toosendan better, and ensure that non-alkaloid component is not destroyed and activity is not suffered a loss.The present invention proposes first ultrasonic, then microwave, finally ultrasonic again method (ultrasonic-microwave-ultrasonic method).Like this, just can control component and activity that the microwave time avoids destroying non-alkaloid, greatly can also improve the extracted amount of non-alkaloid, this method extracts abiotic alkali number from Rhizoma Corydalis and Fructus Toosendan can reach 60-90mg/g, far away higher than conventional water decoction-alcohol sedimentation method, it is its about 6 times.
Table 1 distinct methods extracts the contrast of abiotic alkali number from Rhizoma Corydalis and Fructus Toosendan
Claims (10)
1. from Fructus Toosendan and Rhizoma Corydalis, extract the method for non-alkaloid, it is characterized in that the method extracting non-alkaloid from Fructus Toosendan and Rhizoma Corydalis is carried out according to following steps:
One, the Fructus Toosendan of the Rhizoma Corydalis of 100g and 200g is mixed, then grind, add ethanol water, obtain mixture;
Two, the mixture 10-20 minute that obtains of ultrasound treatment step one, temperature controls at 20-40 DEG C, ultrasonic power 100-200W, and ultrasonic frequency is 40KHz; Then transfer to microwave heating 10-20 minute in microwave reactor, microwave power is 260-520W, and frequency is 2450MHz; Again 20-40 DEG C, ultrasonic power is 100-200W, ultrasonic frequency be the condition of 40KHz under supersound process 10-20 minute, filter, collect filtrate, steam the ethanol in filtrate, add water to 200mL again, the pH value adding hydrochloric acid conditioning solution is 1-2, then crosses 731 type cation exchange resin columns, collects the ion exchange liquid containing non-alkaloid components;
Three, the pH value of the ion exchange liquid containing non-alkaloid components is adjusted, concentrating under reduced pressure, then dry under vacuo, obtained non-alkaloid powder.
2. from Fructus Toosendan and Rhizoma Corydalis, extract the method for non-alkaloid according to claim 1, it is characterized in that in step one, ethanol water mass concentration is 60-80%.
3. according to claim 1 or 2, from Fructus Toosendan and Rhizoma Corydalis, extract the method for non-alkaloid, it is characterized in that the addition of ethanol water in step one is Rhizoma Corydalis and Fructus Toosendan gross mass 4 times.
4. according to claim 1 or 2, from Fructus Toosendan and Rhizoma Corydalis, extract the method for non-alkaloid, it is characterized in that in step one, grinding into 30 object powder.
5. according to claim 1 or 2, from Fructus Toosendan and Rhizoma Corydalis, extract the method for non-alkaloid, it is characterized in that the mixture 11-19 minute that in step 2, ultrasound treatment step one obtains, temperature controls at 22-38 DEG C, ultrasonic power 120-180W, and ultrasonic frequency is 40KHz.
6. according to claim 1 or 2, from Fructus Toosendan and Rhizoma Corydalis, extract the method for non-alkaloid, it is characterized in that the mixture 13-17 minute that in step 2, ultrasound treatment step one obtains, temperature controls at 25-35 DEG C, ultrasonic power 130-260W, and ultrasonic frequency is 40KHz.
7. according to claim 1 or 2, extract the method for non-alkaloid from Fructus Toosendan and Rhizoma Corydalis, it is characterized in that the mixture 15 minutes that ultrasound treatment step one in step 2 obtains, temperature controls at 30 DEG C, ultrasonic power 200W, and ultrasonic frequency is 40KHz.
8. according to claim 1 or 2, extract the method for non-alkaloid from Fructus Toosendan and Rhizoma Corydalis, it is characterized in that then transferring in step 2 with microwave heating 11-18 minute in microwave reactor, microwave power is 280-500W, and frequency is 2450MHz.
9. according to claim 1 or 2, extract the method for non-alkaloid from Fructus Toosendan and Rhizoma Corydalis, it is characterized in that then transferring in step 2 with microwave heating 12-17 minute in microwave reactor, microwave power is 300-450W, and frequency is 2450MHz.
10. according to claim 1 or 2, extract the method for non-alkaloid from Fructus Toosendan and Rhizoma Corydalis, it is characterized in that then transferring in step 2 in microwave reactor and use microwave heating 15 minutes, microwave power is 400W, and frequency is 2450MHz.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018115296A1 (en) | 2016-12-22 | 2018-06-28 | Antofenol | Preparation of a dry biomass extract rich in polyphenols |
| WO2020077755A1 (en) * | 2018-10-16 | 2020-04-23 | 泰州医药城国科化物生物医药科技有限公司 | Method for preparing quaternary ammonium alkaloid component in rhizoma corydalis extract |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20100007520A (en) * | 2008-07-14 | 2010-01-22 | 대구한의대학교산학협력단 | The extract of corydalis turtschaninovii having reinforced pharmaceutical activity, the preparation method thereof and the composition comprising the same |
| CN102920788A (en) * | 2012-11-16 | 2013-02-13 | 黑龙江中医药大学 | Preparation method of non-alkaloid pills extracted from fructus toosendan and rhizoma corydalis for treating gastric ulcer |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20100007520A (en) * | 2008-07-14 | 2010-01-22 | 대구한의대학교산학협력단 | The extract of corydalis turtschaninovii having reinforced pharmaceutical activity, the preparation method thereof and the composition comprising the same |
| CN102920788A (en) * | 2012-11-16 | 2013-02-13 | 黑龙江中医药大学 | Preparation method of non-alkaloid pills extracted from fructus toosendan and rhizoma corydalis for treating gastric ulcer |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018115296A1 (en) | 2016-12-22 | 2018-06-28 | Antofenol | Preparation of a dry biomass extract rich in polyphenols |
| US11517015B2 (en) | 2016-12-22 | 2022-12-06 | Antofenol | Preparation of a dry biomass extract rich in polyphenols |
| WO2020077755A1 (en) * | 2018-10-16 | 2020-04-23 | 泰州医药城国科化物生物医药科技有限公司 | Method for preparing quaternary ammonium alkaloid component in rhizoma corydalis extract |
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