CN104435135B - Method of extracting non-alkaloid from fructus meliae toosendan and rhizoma corydalis - Google Patents
Method of extracting non-alkaloid from fructus meliae toosendan and rhizoma corydalis Download PDFInfo
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Abstract
The invention discloses a method of extracting non-alkaloid from fructus meliae toosendan and rhizoma corydalis, relates to an extraction method of the non-alkaloid and aims at solving the technical problems of the traditional method of extracting the non-alkaloid of low speed, high cost, need for reflux high temperature, complex operation, high cost of extraction process and low extraction efficiency. The method comprises the following steps: mixing the rhizoma corydalis and the fructus meliae toosendan, grinding, adding an ethanol aqueous solution, and carrying out ultrasonic treatment and microwave heating; then carrying out ultrasonic treatment, filtering, collecting a filtrate, steaming out ethanol in the filtrate, adding water to regulate a pH value, and collecting an ion exchange liquid containing the non-alkaloid component; and regulating a pH value to be 7, carrying out vacuum concentration, and drying under vacuum to prepare non-alkaloid powder. Compared with the decocting and alcohol precipitation processes, the method has the following characteristics of rapidness in extraction, high extraction rate, low cost, high efficiency, no need for reflux high temperature, simpleness and feasibility in operation, low cost of the extraction process and remarkable comprehensive economical benefit. The invention belongs to the field of extracting the non-alkaloid.
Description
Technical field
The present invention relates to a kind of extracting method of non-alkaloid.
Background technology
Sichuan Chinaberry Powder is the recipe for the treatment of trusted subordinate's gastral cavilty all pains card, and curative effect preferably, and uses safety, and Fructus Toosendan and Rhizoma Corydalis
Rope is the main two taste medicines of Chinese medicine Sichuan Chinaberry Powder.Research shows the non-alkaloid components of Sichuan Chinaberry Powder extraction on treatment gastric ulcer
Play a major role.Then plus ethanol extraction the method generally extracting non-alkaloid is decocting, and, the method operating process is complicated, consumption
Duration, extraction efficiency are low, the extraction ratio of non-alkaloid is relatively low, are unfavorable for Study on Modernization and the application of Effective Component of Chinese Medicine.
In ultrasound wave and microwave assisted extraction Chinese medicine, chemical composition is considered as a kind of efficient method.But non-alkaloid is carried
Take and at present adopt decocting in water, alcohol precipitation process, but have that extraction rate is slow, cycle length, price are high and the high temperature that need to flow back, complex operation
Problem.
Content of the invention
The invention aims to solving that the existing method extraction rate extracting non-alkaloid is slow, cycle length, price are high
And the high temperature that need to flow back, complex operation, the technical problem of extraction process high cost, there is provided one kind carries from Fructus Toosendan and Rhizoma Corydalis
The method of negated alkaloid.
The method extracting non-alkaloid from Fructus Toosendan and Rhizoma Corydalis follows the steps below:
First, the Fructus Toosendan of the Rhizoma Corydalis of 100g and 200g is mixed, then grind, add ethanol water, obtain mixture；
2nd, the mixture 10-20 minute that ultrasound treatment step one obtains, temperature control at 20-40 DEG C, ultrasonic power
100-200w, ultrasonic frequency is 40khz；It is then transferred in microwave reactor using microwave heating 10-20 minute, microwave power
For 260-520w, frequency is 2450mhz；Again 20-40 DEG C, ultrasonic power be 100-200w, ultrasonic frequency be 40khz
Under the conditions of supersound process 10-20 minute, filter, collect filtrate, steam the ethanol in filtrate, add water to 200ml, salt adding acid is adjusted
The ph value of section solution is 1-2, then crosses 731 type cation exchange resin columns, collects the ion exchange liquid containing non-alkaloid components；
3rd, adjust the ph value of the ion exchange liquid containing non-alkaloid components, concentrating under reduced pressure, be then dried under vacuum, be obtained
Non-alkaloid powder.
In step one, ethanol water volume parts are 60-80%.
The 6-9% that abiotic alkali number is raw materials quality, i.e. every gram of Rhizoma Corydalis and Fructus Toosendan energy are obtained using the inventive method
(method of decocting alcohol extraction, every gram of this Rhizoma Corydalis and Fructus Toosendan mixture multipotency extract non-life to extract non-alkaloid 60-90 milligram
15 milligrams of alkaloids, and need long-time extraction repeatedly).
This method using first supersound process, effectively can be destroyed rapidly Fructus Toosendan and the hard cell wall of Rhizoma Corydalis, make
Ethanol solution is acted on biological components, reaches and makes extraction component hold diffluent purpose；Microwave is a kind of can be rapidly heated
Sasser, next using microwave treatment, can make each group fractionated molecule quickly discharge from Fructus Toosendan and Rhizoma Corydalis.But when long
Between microwave heating can destroy non-alkaloid component molecule, thus losing activity.But only can not be effective by ultrasonic and microwave treatment
Ground extracts non-alkaloid from Fructus Toosendan and Rhizoma Corydalis, and the abiotic alkali number obtaining is very low.Thus, in order to prevent long-time microwave
Process leads to destroy non-alkaloid component and its activity, and this method takes the control microwave time subsequently mixture to be reused
Ultrasonic Treatment.So, the method for the present invention can quickly and efficiently extract the non-alkaloid group in Fructus Toosendan and Rhizoma Corydalis
Part.The method, compared with decocting in water, alcohol precipitation process, has following outstanding feature: extract quick, inexpensive, efficient；Need not be flowed back height
Temperature；Operation is simple；Extraction process low cost, overall economic efficiency is notable；Efficiency high is particularly taken to be significantly larger than water
Boil, alcohol precipitating method, extraction efficiency can reach decocting in water, 4-6 times about of alcohol precipitating method.
Specific embodiment
Technical solution of the present invention is not limited to act specific embodiment set forth below, also includes between each specific embodiment
Combination in any.
Specific embodiment one: the method extracting non-alkaloid from Fructus Toosendan and Rhizoma Corydalis follows the steps below:
First, the Fructus Toosendan of the Rhizoma Corydalis of 100g and 200g is mixed, then grind into the powder of 20-40 mesh, add to account for and prolong
Rhizoma Corydalis and the ethanol water of Fructus Toosendan gross mass 3-5 times, obtain mixture；
2nd, the mixture 10-20 minute that ultrasound treatment step one obtains, temperature control at 20-40 DEG C, ultrasonic power
100-200w, ultrasonic frequency is 40khz；It is then transferred in microwave reactor using microwave heating 10-20 minute, microwave power
For 260-520w, frequency is 2450mhz；Again 20-40 DEG C, ultrasonic power be 100-200w, ultrasonic frequency be 40khz
Under the conditions of supersound process 10-20 minute, filter, collect filtrate, steam the ethanol in filtrate, add water to 200ml, salt adding acid is adjusted
The ph value of section solution is 1-2, then crosses 731 type cation exchange resin columns, collects the ion exchange liquid containing non-alkaloid components；
3rd, adding naoh in the ion exchange liquid containing non-alkaloid components adjusts ph value to 7, obtains neutral becoming containing non-alkaloid
The ion exchange liquid dividing, concentrating under reduced pressure at less than 60 DEG C, then it is dried under vacuum, prepared non-alkaloid powder.
Specific embodiment two: present embodiment and ethanol water body in step one unlike specific embodiment one
Fraction is 60-80%.Other identical with specific embodiment one.
Specific embodiment three: ethanol in step one unlike one of present embodiment and specific embodiment one or two
The addition of aqueous solution is Rhizoma Corydalis and 4 times of Fructus Toosendan gross mass.Other identical one of with specific embodiment one or two.
Specific embodiment four: grind in step one unlike one of present embodiment and specific embodiment one to three
Become the powder of 30 mesh.Other identical one of with specific embodiment one to three.
Specific embodiment five: ultrasonic in step 2 unlike one of present embodiment and specific embodiment one to four
The mixture 11-19 minute that process step one obtains, temperature control at 22-38 DEG C, ultrasonic power 120-180w, ultrasonic wave frequency
Rate is 40khz.Other identical one of with specific embodiment one to four.
Specific embodiment six: ultrasonic in step 2 unlike one of present embodiment and specific embodiment one to five
The mixture 13-17 minute that process step one obtains, temperature control at 25-35 DEG C, ultrasonic power 130-260w, ultrasonic wave frequency
Rate is 40khz.Other identical one of with specific embodiment one to five.
Specific embodiment seven: ultrasonic in step 2 unlike one of present embodiment and specific embodiment one to six
The mixture that process step one obtains 15 minutes, temperature control at 30 DEG C, ultrasonic power 200w, ultrasonic frequency be 40khz.
Other identical one of with specific embodiment one to six.
Specific embodiment eight: in step 2 unlike one of present embodiment and specific embodiment one to seven then
Transfer to and in microwave reactor, use microwave heating 11-18 minute, microwave power is 280-500w, frequency is 2450mhz.Other with
One of specific embodiment one to seven is identical.
Specific embodiment nine: in step 2 unlike one of present embodiment and specific embodiment one to eight then
Transfer to and in microwave reactor, use microwave heating 12-17 minute, microwave power is 300-450w, frequency is 2450mhz.Other with
One of specific embodiment one to eight is identical.
Specific embodiment ten: in step 2 unlike one of present embodiment and specific embodiment one to nine then
Transfer to and use microwave heating 15 minutes in microwave reactor, microwave power is 400w, frequency is 2450mhz.Other with concrete reality
Apply one of mode one to nine identical.
Using following experimental verifications effect of the present invention:
Experiment one:
The method that decoction and alcohol sedimentation technique extracts non-alkaloid follows the steps below:
First, the Fructus Toosendan of the Rhizoma Corydalis of 100g and 200g is mixed, be subsequently adding and account for Rhizoma Corydalis and 8 times of Fructus Toosendan gross mass
Distilled water decoct 2h, collect decoction liquor, add account for Rhizoma Corydalis and 6 times of Fructus Toosendan gross mass distilled water decoct 2h, merge
Decoction liquor simultaneously concentrates, and obtains the concentrated solution of 500ml；
2nd, the ethanol that mass concentration is 95% to volume fraction of ethanol in mixed liquor is added to be 75% in concentrated solution, quiet
Filter after putting 24h, then steam the ethanol in filtrate, add water to 500ml, adjusting ph value in enriching hydrochloric acid to solution is 1, so
Cross 731 type cation exchange resin columns afterwards, collect the ion exchange liquid containing non-alkaloid components, then to containing non-alkaloid components
Add naoh in ion exchange liquid and adjust ph value to neutrality；Concentrating under reduced pressure at 50 DEG C, is then dried under vacuum prepared abiotic ground caustic
End.
Non-alkaloid 10-15 milligram can be extracted using every gram of Rhizoma Corydalis of this method and Fructus Toosendan mixture.
Experiment two:
The method of ultrasonic extraction method non-alkaloid follows the steps below:
By the Fructus Toosendan mixing of the Rhizoma Corydalis of 100g and 200g, then grind into the powder of 20-40 mesh, add and account for Rhizoma Corydalis
Mass concentration with Fructus Toosendan gross mass 3-5 times is 80% ethanol water, soaks 8-12 hour first, then using ultrasonic
Process said mixture 10-20 minute, temperature control at 40 degree, ultrasonic power 100-200w, ultrasonic frequency be 40khz；
Cooling, filtration, then steam the ethanol in filtrate, add water to 500ml, and adjusting ph value in enriching hydrochloric acid to solution is 1, then
Cross 731 type cation exchange resin columns, collect the ion exchange liquid containing non-alkaloid components, then to containing non-alkaloid components from
Son exchanges in liquid plus naoh adjusts ph value to neutrality；Concentrating under reduced pressure at 50 DEG C, is then dried under vacuum prepared non-alkaloid powder.
Non-alkaloid 8-12 milligram can be extracted using every gram of Rhizoma Corydalis of the method and Fructus Toosendan mixture.
Experiment three:
The method of multistage microwave amplifier non-alkaloid follows the steps below:
By the Fructus Toosendan mixing of the Rhizoma Corydalis of 100g and 200g, then grind into the powder of 20-40 mesh, add and account for Rhizoma Corydalis
Mass concentration with Fructus Toosendan gross mass 3-5 times is the ethanol water of 60-80%, is placed in microwave reactor, using micro-
Wave heating 10-20 minute, microwave power is 260-520w, and frequency is 2450mhz.After the completion of said process, mixture while hot
Filter, collect filtrate, then steam the ethanol in filtrate, add water to 200ml, the ph value of salt adding acid-conditioning solution is 1-2, so
Cross 731 type cation exchange resin columns afterwards, collect the ion exchange liquid containing non-alkaloid components, then to containing non-alkaloid components
In ion exchange liquid plus naoh adjusts ph value to 7；The neutral ion exchange liquid containing non-alkaloid components of gained, less than at 60 DEG C
Concentrating under reduced pressure, is then dried under vacuum prepared non-alkaloid powder.
Every gram of Rhizoma Corydalis and Fructus Toosendan can extract non-alkaloid 8-15 milligram.
Experiment four:
The method that ultrasonic-microwave method extracts non-alkaloid follows the steps below:
By the Fructus Toosendan mixing of the Rhizoma Corydalis of 100g and 200g, then grind into the powder of 20-40 mesh, add and account for Rhizoma Corydalis
6 mass concentrations with Fructus Toosendan gross mass 3-5 times are the ethanol water of 0-80%, first by supersound process said mixture
10-20 minute, in 20-40 degree, ultrasonic power 100-200w, ultrasonic frequency is 40khz to temperature control；It is then transferred into micro-
Microwave heating 10-20 minute is used, microwave power is 260-520w, frequency is 2450mhz in ripple reactor.Said process completes
Afterwards, mixture filtered while hot, collect filtrate, then steam the ethanol in filtrate, add water to 200ml, salt adding acid adjusts molten
The ph value of liquid is 1-2, then crosses 731 type cation exchange resin columns, collects the ion exchange liquid containing non-alkaloid components, then to
In ion exchange liquid containing non-alkaloid components plus naoh adjusts ph value to 7；The neutral ion containing non-alkaloid components of gained is handed over
Change liquid, concentrating under reduced pressure at less than 60 DEG C, prepared non-alkaloid powder is then dried under vacuum.
Using the method, every gram of Rhizoma Corydalis and Fructus Toosendan can extract non-alkaloid 22-28 milligram.
Experiment five:
The method that microwave-ultrasonic method extracts non-alkaloid follows the steps below:
By the Fructus Toosendan mixing of the Rhizoma Corydalis of 100g and 200g, then grind into the powder of 20-40 mesh, add and account for Rhizoma Corydalis
Mass concentration with Fructus Toosendan gross mass 3-5 times is the ethanol water of 60-80%, first by microwave heating 10-20 minute,
Microwave power is 260-520w, and frequency is 2450mhz.Then using supersound process said mixture 10-20 minute, temperature control
At 40 degree, ultrasonic power 100-200w, ultrasonic frequency is 40khz；Cooling, filtration, then steam the ethanol in filtrate, then
Add water to 500ml, adjusting ph value in enriching hydrochloric acid to solution is 1, then crosses 731 type cation exchange resin columns, collects containing non-
The ion exchange liquid of alkaloid component, then add naoh tune ph value in the ion exchange liquid containing non-alkaloid components to neutrality；50
Concentrating under reduced pressure at DEG C, is then dried under vacuum prepared non-alkaloid powder.
Non-alkaloid 15-21 milligram can be extracted using every gram of Rhizoma Corydalis of the method and Fructus Toosendan mixture.
Experiment six:
The method (ultrasonic-microwave-ultrasonic method) of extraction non-alkaloid from Fructus Toosendan and Rhizoma Corydalis:
By the mixing of the Fructus Toosendan of the Rhizoma Corydalis of 100g and 200g, then grind into the powder of 30 mesh, add account for Rhizoma Corydalis and
The mass concentration that 5 times of Fructus Toosendan gross mass is 80% ethanol water, first by supersound process said mixture 20 minutes,
Temperature control at 40 DEG C, ultrasonic power 200w, ultrasonic frequency be 40khz；It is then transferred in microwave reactor using microwave
Heating 15 minutes, microwave power is 390w, and frequency is 2450mhz；Finally, then said mixture is placed into ultrasonoscope again
In device, temperature be 40 DEG C, ultrasonic power 200w, ultrasonic frequency be 40khz ultrasound condition under supersound process 20 minutes,
Filter, collect filtrate, steam the ethanol in filtrate, add water to 200ml, the ph value of salt adding acid-conditioning solution is 1, then mistake
731 type cation exchange resin columns, collect the ion exchange liquid containing non-alkaloid components；Hand over to the ion containing non-alkaloid components
Change in liquid plus naoh adjusts ph value to 7, obtain the neutral ion exchange liquid containing non-alkaloid components, reduce pressure at less than 60 DEG C dense
Contracting, is then dried under vacuum, prepared non-alkaloid powder.
This method obtains 9% that abiotic alkali number is raw materials quality, that is, every gram of Rhizoma Corydalis and Fructus Toosendan can extract abiotic
90 milligrams of alkali.
Experiment seven:
The method (ultrasonic-microwave-ultrasonic method) of extraction non-alkaloid from Fructus Toosendan and Rhizoma Corydalis:
By the mixing of the Fructus Toosendan of the Rhizoma Corydalis of 100g and 200g, then grind into the powder of 20 mesh, add account for Rhizoma Corydalis and
The mass concentration that 3 times of Fructus Toosendan gross mass is 60% ethanol water, first by supersound process said mixture 10 minutes,
Temperature control at 40 DEG C, ultrasonic power 100w, ultrasonic frequency be 40khz；It is then transferred in microwave reactor using microwave
Heating 10 minutes, microwave power is 260w, and frequency is 2450mhz；Then said mixture is placed in ultrasonic instrument again,
In temperature control at 40 DEG C, ultrasonic power 100w, supersound process 10 minutes under the ultrasound condition for 40khz for the ultrasonic frequency,
Filter, collect filtrate, steam the ethanol in filtrate, add water to 200ml, the ph value of salt adding acid-conditioning solution is 1, then mistake
731 type cation exchange resin columns, collect the ion exchange liquid containing non-alkaloid components；Hand over to the ion containing non-alkaloid components
Change in liquid plus naoh adjusts ph value to 7, obtain the neutral ion exchange liquid containing non-alkaloid components, reduce pressure at less than 60 DEG C dense
Contracting, is then dried under vacuum, prepared non-alkaloid powder.
This experimental technique obtains 6% that abiotic alkali number is raw materials quality, that is, every gram of Rhizoma Corydalis and Fructus Toosendan can extract non-
60 milligrams of alkaloid.
Experiment eight:
The method (ultrasonic-microwave-ultrasonic method) of extraction non-alkaloid from Fructus Toosendan and Rhizoma Corydalis:
By the mixing of the Fructus Toosendan of the Rhizoma Corydalis of 100g and 200g, then grind into the powder of 40 mesh, add account for Rhizoma Corydalis and
The mass concentration that 4 times of Fructus Toosendan gross mass is 70% ethanol water, first by supersound process said mixture 15 minutes,
Temperature control at 30 DEG C, ultrasonic power 150w, ultrasonic frequency be 40khz；It is then transferred in microwave reactor using microwave
Heating 15 minutes, microwave power is 312w, and frequency is 2450mhz；Finally, then said mixture is placed into ultrasonoscope again
In device, in temperature control at 30 DEG C, ultrasonic power 150w, ultrasonic frequency is supersound process 15 minutes under the conditions of 40khz, mistake
Filter, collects filtrate, steams the ethanol in filtrate, add water to 200ml, the ph value of salt adding acid-conditioning solution is 1, then crosses 731
Type cation exchange resin column, collects the ion exchange liquid containing non-alkaloid components；To the ion exchange containing non-alkaloid components
Adding naoh in liquid adjusts ph value to 7, obtains the neutral ion exchange liquid containing non-alkaloid components, concentrating under reduced pressure at less than 60 DEG C,
Then it is dried under vacuum, prepared non-alkaloid powder.This experimental technique obtains 6.7% that abiotic alkali number is raw materials quality,
I.e. every gram of Rhizoma Corydalis and Fructus Toosendan can extract 78 milligrams of non-alkaloid.
The difference extracting the amount of non-alkaloid using above Different Extraction Method from Rhizoma Corydalis and Fructus Toosendan is shown in Table 1.
In sum, the water decoction-alcohol sedimentation method adopting at present can only extract non-alkaloid from every gram of Rhizoma Corydalis and Fructus Toosendan
10-15mg, we attempt the method using single ultrasound wave or microwave-assisted, can reach the extraction with water decoction-alcohol sedimentation method
Effect, non-alkaloid extracted amount is at most also in 15mg/g；But can reach and shorten extraction time, so that extraction process is become
Simply；On this basis, carried out the ultrasonic non-alkaloid extracting in Rhizoma Corydalis and Fructus Toosendan with microwave combination, after that is, elder generation is ultrasonic
Ultrasonic method (microwave-ultrasonic method) after microwave method (Ultrasound-microwave) and first microwave, both approaches can improve non-life further
The extracted amount of alkaloids, and Ultrasound-microwave is better than microwave-ultrasonic method on non-alkaloid extracted amount, this with ultrasonic can
Effectively destroy Rhizoma Corydalis relevant with the cell wall of Fructus Toosendan.But that the extracted amount of non-alkaloid increases and inconspicuous, test table
Bright further effectively can extract non-alkaloid by extending microwave heating time, but long microwave heating can destroy one
The component of a little non-alkaloids and activity.Therefore, in order to preferably improve the extracted amount of non-alkaloid in Rhizoma Corydalis and Fructus Toosendan, and
Ensure that non-alkaloid component is not destroyed and its activity is not suffered a loss.The present invention proposes first ultrasonic, then microwave, finally surpasses again
The method (ultrasonic-microwave-ultrasonic method) of sound.In this manner it is possible to control the microwave time avoid damage to non-alkaloid component and
Activity moreover it is possible to greatly improve the extracted amount of non-alkaloid, this method extract from Rhizoma Corydalis and Fructus Toosendan abiotic alkali number up to
60-90mg/g, the water decoction-alcohol sedimentation method significantly larger than commonly used, is its 6 times about.
Table 1 distinct methods extract the contrast of abiotic alkali number from Rhizoma Corydalis and Fructus Toosendan
Claims (10)
1. extract the method for non-alkaloid from Fructus Toosendan and Rhizoma Corydalis it is characterised in that extracting non-from Fructus Toosendan and Rhizoma Corydalis
The method of alkaloid follows the steps below:
First, the Fructus Toosendan of the Rhizoma Corydalis of 100g and 200g is mixed, then grind, add ethanol water, obtain mixture；
2nd, the mixture 10-20 minute that ultrasound treatment step one obtains, temperature control at 20-40 DEG C, ultrasonic power 100-
200w, ultrasonic frequency is 40khz；It is then transferred in microwave reactor using microwave heating 10-20 minute, microwave power is
260-520w, frequency is 2450mhz；Again 20-40 DEG C, ultrasonic power be 100-200w, ultrasonic frequency be 40khz bar
Supersound process 10-20 minute under part, filters, and collects filtrate, steams the ethanol in filtrate, adds water to 200ml, salt adding acid is adjusted
The ph value of solution is 1-2, then crosses 731 type cation exchange resin columns, collects the ion exchange liquid containing non-alkaloid components；
3rd, adjust the ph value of the ion exchange liquid containing non-alkaloid components, concentrating under reduced pressure, be then dried under vacuum, non-life is obtained
Alkaloids powder.
2. extract the method for non-alkaloid according to claim 1 from Fructus Toosendan and Rhizoma Corydalis it is characterised in that step one
Middle ethanol water mass concentration is 60-80%.
3. the method for non-alkaloid of extracting from Fructus Toosendan and Rhizoma Corydalis according to claim 1 or claim 2 is it is characterised in that step
In one, the addition of ethanol water is Rhizoma Corydalis and 4 times of Fructus Toosendan gross mass.
4. the method for non-alkaloid of extracting from Fructus Toosendan and Rhizoma Corydalis according to claim 1 or claim 2 is it is characterised in that step
The powder of 30 mesh is ground in one.
5. the method for non-alkaloid of extracting from Fructus Toosendan and Rhizoma Corydalis according to claim 1 or claim 2 is it is characterised in that step
The mixture 11-19 minute that in two, ultrasound treatment step one obtains, temperature control at 22-38 DEG C, ultrasonic power 120-180w,
Ultrasonic frequency is 40khz.
6. the method for non-alkaloid of extracting from Fructus Toosendan and Rhizoma Corydalis according to claim 1 or claim 2 is it is characterised in that step
The mixture 13-17 minute that in two, ultrasound treatment step one obtains, temperature control at 25-35 DEG C, ultrasonic power 130-260w,
Ultrasonic frequency is 40khz.
7. the method for non-alkaloid of extracting from Fructus Toosendan and Rhizoma Corydalis according to claim 1 or claim 2 is it is characterised in that step
The mixture that in two, ultrasound treatment step one obtains 15 minutes, temperature control at 30 DEG C, ultrasonic power 200w, ultrasonic frequency
For 40khz.
8. the method for non-alkaloid of extracting from Fructus Toosendan and Rhizoma Corydalis according to claim 1 or claim 2 is it is characterised in that step
It is then transferred in microwave reactor using microwave heating 11-18 minute in two, microwave power is 280-500w, frequency is
2450mhz.
9. the method for non-alkaloid of extracting from Fructus Toosendan and Rhizoma Corydalis according to claim 1 or claim 2 is it is characterised in that step
It is then transferred in microwave reactor using microwave heating 12-17 minute in two, microwave power is 300-450w, frequency is
2450mhz.
10. the method extracting non-alkaloid from Fructus Toosendan and Rhizoma Corydalis according to claim 1 or claim 2 is it is characterised in that walk
It is then transferred in rapid two in microwave reactor using microwave heating 15 minutes, microwave power is 400w, frequency is 2450mhz.
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