CN104430568A - Botanical pesticide for preventing and treating wolfberry thrips - Google Patents
Botanical pesticide for preventing and treating wolfberry thrips Download PDFInfo
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- CN104430568A CN104430568A CN201410598813.5A CN201410598813A CN104430568A CN 104430568 A CN104430568 A CN 104430568A CN 201410598813 A CN201410598813 A CN 201410598813A CN 104430568 A CN104430568 A CN 104430568A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention relates to a botanical pesticide, and in particular relates to a botanical pesticide for preventing and treating wolfberry thrips. The botanical pesticide is characterized in that a preparation method of the pesticide preparation comprises the following steps: firstly dissolving active compounds into solutions by adding a solvent, mixing the solutions to form a compound liquor, adding an emulsifier in the compound liquor, mixing to form mother solution, dropwise adding water, high-shear mixing and filtering the mother solution to obtain a preparation, wherein the active compound is mixture of nicotine and any one of sophocarpidine, celastrus angulatus, toosendanin, stemonine, veratrine, pyrethrin, azadirachtin or fructus cnidii. With the adoption of the technical scheme, the compounding range of the botanical pesticide is expanded, the efficiency of the botanical pesticide is further improved, and the problems of serious thrips hazards to wolfberry and overproof wolfberry pesticide residue are solved.
Description
Technical field
The present invention relates to a kind of botanical pesticide, especially relate to a kind of botanical pesticide preventing and treating matrimony vine thrips.
Background technology
At present, the thrip fuana that the whole world has described about more than 7400 is planted, point 2 suborder 8 sections, and the thrips about more than 400 of China's record is planted.Thrips is global important omnivorous insect, and based on phytophagy, host plant is extremely extensive, the plants such as the plurality of cereals that mainly causes harm, fruits and vegetables flowers and gardening.This class pest takes food with rasping-sucking mouthparts or lays eggs to cause in the tender epidermal tissue of Young Plant directly causes harm, and causes plant organ to damage, affects photosynthesis, thus affect exterior quality and the output of product, reduce economic worth; Some thrips can also be propagated various plants virus and be caused and indirectly cause harm, and make the viroses of plant popular in a large number between crop.The direct harm of thrips to host plant is less than the indirect hazard that its transmitted virus causes.Thrips class pest build is small, often conceals in plant bud, cause harm and not easily find, and fertility is extremely strong, can break out in a large number in a short time and cause harm, agricultural production mainly adopts chemical control.For a long time, due to the not scientific and reasonable use of chemical pesticide, cause the residue of pesticide of agricultural economy crop to exceed standard, make safety in production face huge challenge.
And botanical pesticide generally has person poultry safety and the advantage less on environmental quality impact, exactly compensate for the defect of chemical pesticide.But botanical pesticide use cost is high, effect is slow, the lasting period is relatively short, and insecticidal spectrum is narrower, and therefore it is promoted, popularizes difficulty certain in addition.
In recent years, matrimony vine thrips becomes one of insect that harm is the most serious, control is the most difficult, in matrimony vine produces with the naked thrips of India for Main Populations, also with flower thrips, rice thrips, magnificent simple pipe thrips and rice letter pipe thrips, have a strong impact on and govern the sound development of wolfberry industry.Therefore thatch agriculture blindly passive use chemical agent, not only do not obtain good control efficiency but also caused again the residual severe overweight problem of agriculture, matrimony vine Product Safety is declined, large quantities of products outlet is limited.
Also do not overcome the method for above-mentioned defect at present.
Summary of the invention
The object of the invention is the defect overcoming prior art, a kind of composite scope that can improve botanical pesticide is provided, be conducive to the high efficiency improving botanical pesticide further, to solve a kind of botanical pesticide preventing and treating matrimony vine thrips of the problems such as the serious and matrimony vine residue of pesticide of matrimony vine thrips harm exceed standard.
The present invention realizes in the following way:
A kind of botanical pesticide preparation preventing and treating matrimony vine thrips, it is characterized in that: the preparation method of this pesticidal preparations is for be first dissolved into solution by former medicine solubilizer, then solution is mixed into compound solution, mother liquor is become after again compound solution being added emulsifier for mixing, finally by mother liquor by dripping water, high shear is stirred, is filtered to obtain preparation, above-mentioned former medicine is any one mixture in nicotine and matrine, Celastrus angulatus, margosine, stemonine, jervine, pyrethrin, nimbin or frutus cnidii;
The mass concentration of described former medicine is followed successively by matrine 15%, Celastrus angulatus 6%, margosine 1%, stemonine 2%, jervine 5%, pyrethrin 25%, nimbin 5%, nicotine 95%, frutus cnidii 30%, the concentration obtaining solution to be mixed after described former medicine dilution is followed successively by print chinaberry 0.01g/L, matrine 0.01g/L, nicotine 0.03g/L, Dalmatian chrysanthemum 0.08g/L, frutus cnidii concentration 0.05g/L, Celastrus angulatus 3g/L, China tree 0.05g/L, jervine 0.35g/L, stemonine 20g/L;
The volume ratio of described solution mixing is: nicotine: print chinaberry=1:5 ~ 5:1, nicotine: matrine=1:5 ~ 5:1, nicotine: frutus cnidii=1:5 ~ 5:1, nicotine: China tree=1:5 ~ 5:1, nicotine: Celastrus angulatus=1:5 ~ 5:1;
Described pharmaceutical formulation following any one:
Volume ratio is: nicotine: print chinaberry=1:5 ~ 5:1+20% solvent+8% emulsifier, and pure water complements to 100%;
Volume ratio is: nicotine: matrine=1:5 ~ 5:1+20% solvent+8% emulsifier, and pure water complements to 100%;
Volume ratio is: nicotine: frutus cnidii=1:5 ~ 5:1+25% solvent+8% emulsifier, and pure water complements to 100%;
Volume ratio is: nicotine: China tree=1:5 ~ 5:1+18% solvent+8% emulsifier, and pure water complements to 100%;
Volume ratio is: nicotine: Celastrus angulatus=1:5 ~ 5:1+35% solvent+8% emulsifier, and pure water complements to 100%;
Above-mentioned content is volumn concentration;
Described solvent is acetone, alcohol or water, and described emulsifier is dense newborn 500, OP-10 or Tween 80.
The present invention has following effect:
The experimental technique used in following embodiment if no special instructions, is conventional method.
Material used in following embodiment, reagent etc., if no special instructions, all can obtain from commercial channels.
Embodiment 1,9 Plants source desinsection single dose is to the Toxicity Determination of thrips
Examination worm: matrimony vine thrips picks up from field, gardens, Yinchuan of Ningxia Province city, through being accredited as compound population.In indoor, continuous band is raised, and gets matrimony vine thrips two nymph in age for examination.
Reagent agent: 15% matrine, 6% Celastrus angulatus, 1% margosine, 2% stemonine, 5% jervine, 25% pyrethrin, 5% nimbin, 95% nicotine, 30% frutus cnidii, the source of this raw material is bright biological development corporation, Ltd. of Baoji side;
Solvent acetone, emulsifier Tween-80, makes through stirring.
Assay method: adopt medicine film leaf dipping method, concrete steps are as follows:
(1) first by above-mentioned 9 Plants source medicament dissolution with solvents, then be diluted to some gradients with water-emulsifier, ensure that having prepared liquid is transparent, finely dispersed concentration of participating in the experiment.After preparing liquid, first make insect box medicine film: after medicine based on herbs is added acetone solution, be diluted to some concentration with water-emulsifier, even spraying in insect box, Indoor Natural dries makes medicine film.
(2) again with identical liquor strength dipping matrimony vine blade, until after medicine film and leaching medicine blade dry, band medicine blade is put into medicine film insect box, forms medicine film leaf dipping method.Finally, access is participated in the experiment and is tried worm about 30, and cultivates observation, calculates lethality.
With water box and leaching clear water matrimony vine blade as a control group, every concentration process 4 repetition.Control group lethality < 10% is efficiency test.
Study on Fungicide Toxicity: utilize DPS analysis software to carry out data processing, obtains the virulence regression equation of a medicament, LC50 value and 95% confidence limit thereof etc.
Malicious factor >20 altogether, representing that two single doses are mixed has synergistic effect; ≤-20 indicate antagonism, and-20 ~ 20 represent summation action.
The Toxicity Determination of table 19 Plants source medicament
Can be learnt by the data of table 1, print chinaberry, matrine and the LC50 of nicotine to thrips are lower, and LC50 is lower, and Contact toxicity is stronger, therefore selects these 3 kinds of medicaments and other 6 Plants source medicaments to carry out composite, and investigate the Contact toxicity to thrips.
Embodiment 2, print chinaberry and other 8 Plants source medicament are to the Toxicity Determination of thrips
Be that 5:1,2:1,1:1,1:2,1:5 carry out binary mixture and obtain Mixed Pharmacy (each drug concentration is LC50 value, prints chinaberry: 0.01g/L by the botanical pesticide screened in embodiment 1 print chinaberry and other 8 kinds of former medicines according to volume ratio; Matrine: 0.01g/L; Nicotine: 0.03g/L; Dalmatian chrysanthemum: 0.08g/L; Frutus cnidii: 0.05g/L; Celastrus angulatus: 3g/L; China tree: 0.05g/L; Jervine: 0.35g/L; Stemonine: 20g/L).Investigate 8 kinds of Mixed Pharmacies to the Toxicity Determination of thrips, adopt medicine film leaf dipping method to measure, concrete steps are with in embodiment 1.
The joint toxicity measuring result of above-mentioned 8 kinds of Mixed Pharmacies is as shown in table 2.
Table 2 prints the binary mixture toxicity test of chinaberry and other 8 Plants source medicament
Data as can be seen from table 2, print chinaberry: matrine=1:5 mixture, the malicious factor is 31.08 > 20 altogether, there is synergistic effect, the malicious factor is larger altogether, synergistic effect is more obvious, shows that its synergistic effect is the most obvious, therefore print chinaberry and the insecticidal activity of matrine two kinds of medicament mixing ratio single doses to thrips high; Print chinaberry: nicotine=1:5 ~ 2:1 mixture, altogether the malicious factor is all greater than 20, shows that its mixture all has synergistic effect, print chinaberry and the insecticidal activity of nicotine two kinds of medicament mixing ratio single doses to thrips high; Wherein print chinaberry: nicotine=1:2, the malicious factor is maximum altogether, reaches 54.77, shows that synergistic effect is tagged the most obviously in the indoor of this Mixed Pharmacy to thrips, and print chinaberry and nicotine two kinds of medicaments mixing, ratio is that the contact toxicity of 1:2 to thrips is the highest.
Embodiment 3, be that 5:1,2:1,1:1,1:2,1:5 carry out binary mixture and obtain Mixed Pharmacy by botanical pesticide matrine and other 7 kinds of former medicines according to volume ratio.Investigate 7 kinds of Mixed Pharmacies to the Toxicity Determination of thrips, adopt medicine film leaf dipping method to measure, concrete steps are with in embodiment 1.
The joint toxicity measuring result of above-mentioned 7 kinds of Mixed Pharmacies is as shown in table 3.
The binary mixture toxicity test of table 3 matrine and other 7 Plants source medicament
Data as can be seen from table 3, matrine: nicotine=1:5 ~ 5:1 mixture, altogether the malicious factor is all greater than 20, shows that its mixture has synergistic effect, therefore when matrine and nicotine composite according to this ratio after, Be very effective is higher than the effect of each single dose.Particularly matrine: during nicotine=5:1, the malicious factor is 48.03 to the maximum altogether, shows that its mixed synergies is the most remarkable, the most remarkable to thrips activity.
Consolidated statement 2 and table 3, through a large amount of compound screening, can obtain nicotine synergistic effect in control matrimony vine thrips remarkable, therefore carry out composite to nicotine and other 8 Plants source medicaments.
The Toxicity Determination of embodiment 4, nicotine and all the other 8 Plants source medicaments
The binary mixture toxicity test of table 4 nicotine and other 6 Plants source medicament
Reached a conclusion by the data in table 4, nicotine: frutus cnidii=1:5 ~ 5:1, the malicious factor is all greater than 20 altogether, shows that its mixture has synergistic effect; Nicotine: China tree=1:5 ~ 5:1, the malicious factor is all greater than 20 altogether, shows that its mixture has synergistic effect; Nicotine: Dalmatian chrysanthemum=1:5 ~ 5:1, the malicious factor is all greater than 20 altogether, shows that its mixture has synergistic effect; Nicotine: jervine=1:5 ~ 5:1, the malicious factor is all greater than 20 altogether, shows that its mixture has synergistic effect; Nicotine: Celastrus angulatus=1:5 ~ 5:1, the malicious factor is all greater than 20 altogether, shows that its mixture has synergistic effect; Nicotine: stemonine=1:5 ~ 5:1, the malicious factor is all greater than 20 altogether, and show that its mixture has synergistic effect, therefore in whole compound process, nicotine plays synergistic effect, and the effect of other 8 kinds of composite reagent control thrips is all higher than the result of use of each single dose.
Associative list 2,3,4 and the composite factor such as reagent cost and plant resources amount can draw the following conclusions:
Described component volume percentage: nicotine: print chinaberry=2:1, all the other are solvent-water-emulsifier
Described component volume percentage: nicotine: matrine=1:5, all the other are water-emulsifier
Described component volume percentage: nicotine: frutus cnidii=5:1, all the other are solvent-water-emulsifier
Described component volume percentage: nicotine: China tree=5:1, all the other are water-emulsifier
Described component volume percentage: nicotine: Celastrus angulatus=5:1, all the other are solvent-water-emulsifier
Serious for matrimony vine thrips harm in matrimony vine residue of pesticide excessive problem can being found out from above-mentioned experiment, provide a kind of, to matrimony vine thrips, there is synergistic effect of tagging significantly, providing new technical support for preventing and treating matrimony vine thrips in production.Nicotine is respectively with print chinaberry, matrine, frutus cnidii, China tree, Celastrus angulatus is composite all has significant synergistic effect, these 5 kinds of complex preparations: 10 kinds of proportionings all higher than 95%, wherein work as nicotine: print chinaberry=2:1 to matrimony vine thrips effect lethality; Nicotine and frutus cnidii=5:1; Nicotine: China tree=5:1,2:1,1:5; Nicotine: during print chinaberry=1:5, its lethality reaches 100%, to sum up these 10 kinds of complex preparation control thrips effects are all higher than the result of use of each single dose.The Toxicity Determination of the comprehensive 10 former medicine proportionings in Plants source and field trial, obtain effect as follows: under the condition of dilution 200 times, all reach more than 95% to matrimony vine thrips indoor virulence; And the field efficacy of 1 day reaches 85% after its medicine, within after medicine 3 days, reach 90%, after medicine, 5 days preventive effects continue to reach 90%, and after 7 days, preventive effect is still 90%, and therefore this 10 Plants source former medicine proportioning is remarkable to matrimony vine thrips preventive effect.
Embodiment
Embodiment 1: a kind of botanical pesticide preparation preventing and treating matrimony vine thrips, the preparation method of this pesticidal preparations is for be first dissolved into solution by former medicine solubilizer, then solution is mixed into compound solution, mother liquor is become after again compound solution being added emulsifier for mixing, finally by mother liquor by dripping water, high shear is stirred, is filtered to obtain preparation, above-mentioned former medicine is any one mixture in nicotine and matrine, Celastrus angulatus, margosine, stemonine, jervine, pyrethrin, nimbin or frutus cnidii; The mass concentration of described former medicine is followed successively by matrine 15%, Celastrus angulatus 6%, margosine 1%, stemonine 2%, jervine 5%, pyrethrin 25%, nimbin 5%, nicotine 95%, frutus cnidii 30%, the concentration obtaining solution to be mixed after described former medicine dilution is followed successively by print chinaberry 0.01g/L, matrine 0.01g/L, nicotine 0.03g/L, Dalmatian chrysanthemum 0.08g/L, frutus cnidii concentration 0.05g/L, Celastrus angulatus 3g/L, China tree 0.05g/L, jervine 0.35g/L, stemonine 20g/L.
The volume ratio of described solution mixing is nicotine: print chinaberry=1:5, and described preparation is that pure water complements to 100% by mixed solution+20% solvent+8% emulsifier;
Embodiment 2: all the other are identical with embodiment 1
The volume ratio of described solution mixing is nicotine: print chinaberry=5:1, and described preparation is that pure water complements to 100% by mixed solution+20% solvent+8% emulsifier;
Embodiment 3: all the other are identical with embodiment 1
The volume ratio of described solution mixing is nicotine: matrine=1:5, and described preparation is that pure water complements to 100% by mixed solution+20% solvent+8% emulsifier;
Embodiment 4: all the other are identical with embodiment 1
The volume ratio of described solution mixing is nicotine: matrine=5:1, and described preparation is that pure water complements to 100% by mixed solution+20% solvent+8% emulsifier;
Embodiment 5: all the other are identical with embodiment 1
The volume ratio of described solution mixing is nicotine: frutus cnidii=1:5, and described preparation is that pure water complements to 100% by mixed solution+25% solvent+8% emulsifier;
Embodiment 6: all the other are identical with embodiment 1
The volume ratio of described solution mixing is nicotine: frutus cnidii=5:1, and described preparation is that pure water complements to 100% by mixed solution+25% solvent+8% emulsifier;
Embodiment 7: all the other are identical with embodiment 1
The volume ratio of described solution mixing is nicotine: China tree=1:5, and described preparation is that pure water complements to 100% by mixed solution+18% solvent+8% emulsifier;
Embodiment 8: all the other are identical with embodiment 1
The volume ratio of described solution mixing is nicotine: China tree=5:1, and described preparation is that pure water complements to 100% by mixed solution+18% solvent+8% emulsifier;
Embodiment 9: all the other are identical with embodiment 1
The volume ratio of described solution mixing is nicotine: Celastrus angulatus=1:5, and described preparation is that pure water complements to 100% by mixed solution+35% solvent+8% emulsifier;
Embodiment 10: all the other are identical with embodiment 1
The volume ratio of described solution mixing is nicotine: Celastrus angulatus=5:1, described preparation is that pure water complements to 100% by mixed solution+35% solvent+8% emulsifier.
Above-mentioned solvent is acetone, alcohol or water, and described emulsifier is dense newborn 500, OP-10 or Tween 80.
Claims (5)
1. prevent and treat the botanical pesticide preparation of matrimony vine thrips for one kind, it is characterized in that: the preparation method of this pesticidal preparations is for be first dissolved into solution by former medicine solubilizer, then solution is mixed into compound solution, mother liquor is become after again compound solution being added emulsifier for mixing, finally by mother liquor by dripping water, high shear is stirred, is filtered to obtain preparation, above-mentioned former medicine is any one mixture in nicotine and matrine, Celastrus angulatus, margosine, stemonine, jervine, pyrethrin, nimbin or frutus cnidii.
2. a kind of botanical pesticide preventing and treating matrimony vine thrips as claimed in claim 1, it is characterized in that: the mass concentration of described former medicine is followed successively by matrine 15%, Celastrus angulatus 6%, margosine 1%, stemonine 2%, jervine 5%, pyrethrin 25%, nimbin 5%, nicotine 95%, frutus cnidii 30%, the concentration obtaining solution to be mixed after described former medicine dilution is followed successively by print chinaberry 0.01g/L, matrine 0.01g/L, nicotine 0.03g/L, Dalmatian chrysanthemum 0.08g/L, frutus cnidii concentration 0.05g/L, Celastrus angulatus 3g/L, China tree 0.05g/L, jervine 0.35g/L, stemonine 20g/L.
3. a kind of botanical pesticide preventing and treating matrimony vine thrips as claimed in claim 1 or 2, it is characterized in that: the volume ratio of described solution mixing is: nicotine: print chinaberry=1:5 ~ 5:1, nicotine: matrine=1:5 ~ 5:1, nicotine: frutus cnidii=1:5 ~ 5:1, nicotine: China tree=1:5 ~ 5:1, nicotine: Celastrus angulatus=1:5 ~ 5:1.
4. a kind of botanical pesticide preventing and treating matrimony vine thrips as claimed in claim 3, is characterized in that: described pharmaceutical formulation following any one:
Volume ratio is: nicotine: print chinaberry=1:5 ~ 5:1+20% solvent+8% emulsifier, and pure water complements to 100%;
Volume ratio is: nicotine: matrine=1:5 ~ 5:1+20% solvent+8% emulsifier, and pure water complements to 100%;
Volume ratio is: nicotine: frutus cnidii=1:5 ~ 5:1+25% solvent+8% emulsifier, and pure water complements to 100%;
Volume ratio is: nicotine: China tree=1:5 ~ 5:1+18% solvent+8% emulsifier, and pure water complements to 100%;
Volume ratio is: nicotine: Celastrus angulatus=1:5 ~ 5:1+35% solvent+8% emulsifier, pure water complements to 100%
Above-mentioned content is volumn concentration.
5. a kind of botanical pesticide preventing and treating matrimony vine thrips as claimed in claim 1, is characterized in that: described solvent is acetone, alcohol or water, and described emulsifier is dense newborn 500, OP-10 or Tween 80.
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