CN106359459A - Pyrethrin and sophocarpidine compounded agent and application thereof - Google Patents
Pyrethrin and sophocarpidine compounded agent and application thereof Download PDFInfo
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- CN106359459A CN106359459A CN201610766903.XA CN201610766903A CN106359459A CN 106359459 A CN106359459 A CN 106359459A CN 201610766903 A CN201610766903 A CN 201610766903A CN 106359459 A CN106359459 A CN 106359459A
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- pyrethrin
- matrine
- agent
- grain
- sophocarpidine
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
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- Health & Medical Sciences (AREA)
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- Agronomy & Crop Science (AREA)
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- Environmental Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pest Control & Pesticides (AREA)
- Botany (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a pyrethrin and sophocarpidine compounded agent. The compounded agent is prepared through compounding pyrethrin and sophocarpidine according to the mass ratio of (3: 1) to (1: 25). The invention further discloses an application of the pyrethrin and sophocarpidine compounded agent in prevention and treatment of foodstuffs storage pests. The pyrethrin and sophocarpidine compounded agent disclosed by the invention has an obvious synergistic effect, and the pesticidal toxicity is apparently higher than that of any single agent; in a foodstuff mixed test, 21 days later, the control efficiency of the compounded agent on significant foodstuffs storage pests such as tribolium castaneum, Sitophilus zeamais and Rhyzopertha dominica reaches 100%.
Description
Technical field
The invention belongs to View of Grain Storage Technoiogy field is and in particular to a kind of pyrethrin and matrine compound agent and its should
With.
Background technology
Grain is the most basic means of livelihood needed for the mankind survive, and food problem is to be related to national state's meter
The cardinal task of the people's livelihood, to nation's security with stablize significant.At present, world's cultivated land resource is limited, grain disappears
Under the situation that expense demand continues to increase, strengthen the safe storage of grain, be to ensure that the important measures of national food security.Grain exists
The main performance causing grain storage loss in storage is the minimizing of Grain Quantity and the reduction of grain quality, and insect pest and mouldy
It is then two key factors causing grain heavy losses in storage.
Grain storage pest is the general name of the harmful insect of harm grain reservation and products thereof.According to statistics, current whole world grain storage
Insect there are about kind more than 500, and the grain storage pest being distributed in China just has kind more than 200.The presence of grain storage pest not only results in grain
The loss of eclipse number amount, also can cause the change of grain quality, even result in and produce the toxin with strong toxicity and carcinogenecity, from
And endanger the healthy of the mankind.In addition, the corpse of insect and Excreta also pollute grain, the quality of grain is made to reduce.According to
The statistics of FAO (Food and Agriculture Organization of the United Nation), the whole world accounts for the 4%～8% of gross storage capacity because of the loss that grain storage pest causes every year, has
National grain storage loss even as high as 30%.Therefore, grain storage pest is effectively prevented and treated and control and guarantee the quality field in grain storage detraction
Have important practical significance.
At present, China's grain storage pest controlling mainly uses phosphine fumigation insecticidal technology.But long-term, single, mistake
Degree has had resulted in serious " 3r " phenomenon (pest resistance, harmful organism wildness and pesticide residues again) using chemical insecticide.As
Fruit can not search out a kind of efficient, green, safe medicament to replace proemial chemical insecticide, then, futureization
The power learning insecticide can weaken or even can the situation to some grain storage pests no preventive and therapeutic effect for the chemical insecticide successively.Cause
This, the research for biological source stored-grain protectant is just particularly important.
Pyrethrum is perennial catananche, plant height 40-70cm, well developed root system, originates from the Middle East and Near East area,
19 beginnings of the century usually prevented and treated the domestic hygiene insect such as mosquito, flea, Blatta seu periplaneta with regard to someone with Pyrethrum.In western countries such as the U.S.,
Pyrethrin has become one of the most frequently used domestic hygiene pests insecticide.
Natural pyrethrum is natural plant insecticide, has efficient knockdown effect to various pests, its advantage
A lot, be mainly manifested in insecticidal spectrum wide, based on tagging, degradable, noresidue, pollution-free, extremely low to person poultry toxicity.Research table
Bright, 1.5% natural pyrethrum all has certain contact efficacy to sitophilus zea-maises, lesser grain borer, red flour beetle.
Radix Sophorae Flavescentiss (sophora flavescens) are pulse family Sophora herbaceos perennials, are all distributed throughout the country,
There is bitter in the mouth, cold in nature, have the effect of heat clearing and damp drying, parasite killing, diuresis.Matrine is a kind of biological chemistry of of Radix Sophorae Flavescentiss plant source itself
Composition, is the similar substance of plant endogenous hormones, has promotion growth, the effect of suppression funguses to crops.
Matrine has lot of advantages, such as safety and low toxicity, noresidue, nuisanceless, no Drug resistance, and insecticidal effect is efficiently, long
Effect etc..The research such as He Lili finds, prevents and treats the vegetable aphid on Chinese cabbage with 0.36% matrine as and has comparatively ideal effect,
The preventive effect of 750ml/ hectare dispenser 3d, 7d, 14d reaches 88.5%, 92.7%, 90.1% respectively；630ml/ hectare dispenser 3d, 7d,
The preventive effect of 14d reaches 88.3%, 93.2%, 89.7% respectively.The application l% matrine soluble liquid such as Fu Zhenke dilutes 1200 times
Afterwards, take liquid spray pattern dispenser preventing and treating Semen Juglandiss to sew leaf snout moth's larva, there is preferable effect, preventive effect reaches 91%.Li Chunyu etc. is pacified
Find in the research of full preventing and treating Eriocheir sinensiss water in field rice pest technology, to Lissorhoptrusoryzophilus, first-class rice grub has preferably l% aqueous matrine solution
Prevention effect, 750ml/hm2After dispenser, the 1st, 3,5,10 days preventive effects are respectively 96.1%, 95.9%, 96.3%, 4.2%；
900ml/hm2After dispenser, the 1st, 3,5,10 days preventive effects are respectively 96.1%, 98.0%, 98.1%, 94.2%.Wu Chuanwan etc. studies
Show, with 1.1% matrine powder, can effectively prevent and treat the insects such as cotton red spider, Li aphid, Pieris rapae, rice-stem borer, to work
Thing nonhazardouss affect.It is because existing Radix Sophorae Flavescentiss alkali test is primarily directed to the Pest control of field and woodland and respond well,
But study in grain storage pest controlling and lack, therefore matrine is also very wide in the application space of grain storage pest controlling.
Although clearly existing fragmentary research shows, pyrethrin has certain prevention effect to some grain storage pests,
But have such problems as that insecticidal spectrum is narrower, use cost is higher, have impact on its practical application.
Content of the invention
The technical problem to be solved is to provide a kind of pyrethrin for preventing and treating grain storage pest and matrine
Built agent and its application.
The present invention is compounded with matrine by pyrethrin, and in built agent, pyrethrin and the mass ratio of matrine are
3:1～1:25.
Preferably, in built agent, pyrethrin and the mass ratio of matrine are 1:2～1:7.
It is highly preferred that pyrethrin and the mass ratio of matrine are 1:2～1:5 in built agent.
Pyrethrin and matrine compound agent are used for the pesticide control of foodstuff preservation by the present invention, and grain storage pest is had relatively
Good prevention effect.
The pyrethrin of the present invention and matrine compound agent have obvious potentiation, and insecticidal toxicity is apparently higher than any
One single dose；Mix in grain test, after 21d, this built agent reaches to important grain storage pest red flour beetle, sitophilus zea-maises and lesser grain borer preventive effect
100%.
Specific embodiment
Below in conjunction with specific embodiment, the present invention is further detailed explanation.
In this specific embodiment, it is lesser grain borer, sitophilus zea-maises and red flour beetle for examination insect, by Wuhan Polytechnic University grain storage elder brother
Worm receptacle provides.Lesser grain borer and sitophilus zea-maises are raised with the Intact wheat of water content 13% ± 1%, and red flour beetle whole wheat flour is raised
Support.Test worm raising temperature is (28 ± 1) DEG C, and relative humidity is (75 ± 5) %, with the adult after sprouting wings two weeks for examination.For examination
In medicament, acetone is purchased from Tianjin Heng Fa chemical reagent company limited, and analysis is pure；Pyrethrin is purchased from the many spaces in Guangdong many biologies section
Skill company limited, purity is 1.5%；Matrine is purchased from Tianjin Hengyuan Biological Technology Development Co., Ltd., and purity is
1.3%.
Embodiment 1
Virulence ratio method screens the potentiation proportioning of pyrethrin and matrine.Lc based on single dose50Value carries out compound synergic
Proportioning is screened.Prepare the lc of two kinds of single doses50Value concentration solution, with the ratio of its volume as 10:0,9:1,8:2,7:3,6:4,
The different proportions such as 5:5,4:6,3:7,1:9,0:10 mix, and are somebody's turn to do divided by expected effective percentage with the actual measurement effective percentage of a certain combination
The virulence ratio of combination, then with the virulence of this combination than the average virulence divided by two single doses than the correction virulence ratio obtaining this combination.
Correction virulence is potentiation hence it is evident that being antagonism less than 1 than significantly greater than 1, is summation action equal to 1.Test result is such as
Table 1.
Verify that the proportioning that virulence screens than method has potentiation with co-toxicity coefficient method, test result such as table 2.
Involved computing formula is as follows:
The lc of expected mortality=a50Dosage actual × paThe lc of+b50Dosage actual × pb
In above formula, pa、pbIt is respectively single dose a (pyrethrin) and single dose b (matrine) for the percentage rate in proportioning.
Table 1 pyrethrin and the screening of matrine potentiation optimum proportioning
Table 2 pyrethrin and matrine compound agent 72h are to sitophilus zea-maises virulence and co-toxicity coefficient
, under given different ratio, correction virulence ratio is all for pyrethrin and the built agent of matrine as can be seen from Table 1
It is significantly greater than 1, there is potentiation.From table 2 it can be seen that pyrethrin and matrine are when mass ratio is for 1:3, its common poison system
Number is for 177.7 (more than 120) hence it is evident that having potentiation.
Embodiment 2
Storehouse preventive effect experiment is simulated to pyrethrin and matrine compound agent (effective mass than for 1:3), and measures this
Built agent prevents and treats minimum using dosage and its prevention effect of important grain storage pest.
Carry out control effect testing using mixing grain method, drug concentration is according to the mass ratio meter of the effective ingredient in medicament and grain
Calculate, according to preliminary result, sets pyrethrin and matrine (mass ratio as 1:3) effective ingredient be set to 1.0mg/kg,
1.5mg/kg, 2.0mg/kg, 2.5mg/kg and 3.0mg/kg totally 5 concentration for the treatment of, and set 1 blank control group.At each
Reason concentration is mixed homogeneously with 200g Semen Tritici aestivi, is then charged in 500ml Cans, is respectively connected to red flour beetle and sitophilus zea-maises each 30
Head, is sealed with gauze.Each process is repeated 3 times, and is placed in 27 DEG C of temperature, carries out reality in the growth cabinet of relative humidity 75%
Test.After processing 21d, investigate the insect death condition that various built agents are processed respectively, and calculate corrected mortality, death standard
It is gently to touch test worm with little brush pen, its feeler and each person motionless to foot are considered as death.After 15d has investigated, test worm is whole
Take out.Wherein, corrected mortality=(processing mortality rate-comparison mortality rate)/(1- compares mortality rate) * 100%.
Data is analyzed with spss17.0, calculates respective ld50Value, ld95Value.For examination insect corrected mortality it is
100% drug dose is effective using dosage, and in the dosage tested, at least 1 dosage is dead to the correction of insect
Rate is less than 100%, at least 2 dosage are 100% to the corrected mortality of insect, then, Mortality of insect is when 100%
Lowest dose level is minimum effective using dosage of this medicament.Test result is shown in Table 3.
From table 3 it can be seen that chemicals treatment is after 21 days, pyrethrin and matrine compound agent are 2.0mg/ in concentration for the treatment of
During more than kg, sitophilus zea-maises and red flour beetle mortality rate all reach 100%.Therefore, pyrethrin is minimum with matrine compound agent makes
Could be arranged to 2.0mg/kg with concentration.
Table 3 pyrethrin is measured with the minimum concentration of matrine compound agent
Note: result is the mean+SD (n=3) of three repetitions；: different lower case representatives after data is processed
Between significant difference (dancan multiple comparisons, p < 0.05)
It is 2.0mg/kg that setting pyrethrin and matrine compound agent (1:3) and two of which single dose mix grain dosage, will be each
Individual concentration for the treatment of is mixed homogeneously with 500g Semen Tritici aestivi, is then charged in 500ml Cans, each process access red flour beetle, sitophilus zea-maises,
Each 30 of lesser grain borer, is sealed with gauze.Each process is repeated 3 times, and sets and do not mix medicine grain and connect worm as blank, is placed in temperature
27 DEG C, observed in the growth cabinet of relative humidity 75%.The death of insect is investigated after processing 7d, 15d, 21d
Situation simultaneously calculates corrected mortality, and death standard is gently to touch test worm with little brush pen, and its feeler and each person motionless to foot regard
For death.Relatively built agent and the insecticidal activity of wherein single dose, are listed in table 4.
From table 4, it can be seen that 7d, 15d and 21d are substantially less than built agent with the insecticidal effect of the single dose of dosage.Illustrate to touch
Kill the synergistic complex agent that measuring goes out under the conditions of mixing grain using equally having potentiation.This is compounding bright to lesser grain borer effect
Aobvious, secondly it is sitophilus zea-maises, worst to red flour beetle effect.After 21 days, can effectively kill the above-mentioned 3 kinds of important grain storages in grain
Insect.
Table 4 pyrethrin insecticidal activity in simulation storehouse with matrine compound agent and its single dose
Claims (4)
1. a kind of pyrethrin and matrine compound agent it is characterised in that: this built agent presses quality by pyrethrin with matrine
Form than compounding for 3:1～1:25.
2. pyrethrin according to claim 1 and matrine compound agent it is characterised in that: this built agent is by pyrethrin
It is that 1:2～1:7 is compounding in mass ratio with matrine to form.
3. pyrethrin according to claim 2 and matrine compound agent it is characterised in that: this built agent is by pyrethrin
It is that 1:2～1:5 is compounding in mass ratio with matrine to form.
4. the pyrethrin described in any one of claims 1 to 3 and matrine compound agent are in the application preventing and treating grain storage insect.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610766903.XA CN106359459B (en) | 2016-08-30 | 2016-08-30 | Pyrethrin and matrine compound agent and its application |
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| CN201610766903.XA CN106359459B (en) | 2016-08-30 | 2016-08-30 | Pyrethrin and matrine compound agent and its application |
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| CN106359459B CN106359459B (en) | 2019-08-16 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107372624A (en) * | 2017-08-01 | 2017-11-24 | 广西南宁市宾阳县聚丰米业有限公司 | A kind of grain storage pest controlling agent containing matrine and oil of badian |
| CN108770884A (en) * | 2018-07-10 | 2018-11-09 | 来安县薜必英家庭农场 | A kind of persistent pesticide of water resistant rice leaf roller |
| CN108770842A (en) * | 2018-05-31 | 2018-11-09 | 湖北归然生物科技有限公司 | The preparation method of high efficiency high stable 2% pyrethrin and matrine compound microemulsion |
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| CN1833504A (en) * | 2005-03-14 | 2006-09-20 | 魏立文 | Prepn. process for high efficiency high stable pyrethrum emulsion |
| CN104146009A (en) * | 2014-07-14 | 2014-11-19 | 卞佳林 | Composite insecticide special for fruit trees and preparation method for composite insecticide |
| CN104430568A (en) * | 2014-10-30 | 2015-03-25 | 宁夏农林科学院 | Botanical pesticide for preventing and treating wolfberry thrips |
| CN104430650A (en) * | 2014-12-01 | 2015-03-25 | 苏州市相城区盛胡特种养殖专业合作社 | Compound pesticide composition |
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2016
- 2016-08-30 CN CN201610766903.XA patent/CN106359459B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1833504A (en) * | 2005-03-14 | 2006-09-20 | 魏立文 | Prepn. process for high efficiency high stable pyrethrum emulsion |
| CN104146009A (en) * | 2014-07-14 | 2014-11-19 | 卞佳林 | Composite insecticide special for fruit trees and preparation method for composite insecticide |
| CN104430568A (en) * | 2014-10-30 | 2015-03-25 | 宁夏农林科学院 | Botanical pesticide for preventing and treating wolfberry thrips |
| CN104430650A (en) * | 2014-12-01 | 2015-03-25 | 苏州市相城区盛胡特种养殖专业合作社 | Compound pesticide composition |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107372624A (en) * | 2017-08-01 | 2017-11-24 | 广西南宁市宾阳县聚丰米业有限公司 | A kind of grain storage pest controlling agent containing matrine and oil of badian |
| CN108770842A (en) * | 2018-05-31 | 2018-11-09 | 湖北归然生物科技有限公司 | The preparation method of high efficiency high stable 2% pyrethrin and matrine compound microemulsion |
| CN108770884A (en) * | 2018-07-10 | 2018-11-09 | 来安县薜必英家庭农场 | A kind of persistent pesticide of water resistant rice leaf roller |
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| CN106359459B (en) | 2019-08-16 |
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