CN104429612A - Bottle cultivation method for ombre mushroom - Google Patents

Bottle cultivation method for ombre mushroom Download PDF

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Publication number
CN104429612A
CN104429612A CN201410756372.7A CN201410756372A CN104429612A CN 104429612 A CN104429612 A CN 104429612A CN 201410756372 A CN201410756372 A CN 201410756372A CN 104429612 A CN104429612 A CN 104429612A
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China
Prior art keywords
mushroom
bottle
weight portion
days
mycelia
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CN201410756372.7A
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Chinese (zh)
Inventor
何珍祖
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Ming Zhen Source Shanglin County Ramulus Mori Bacterium Industry Co Ltd
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Ming Zhen Source Shanglin County Ramulus Mori Bacterium Industry Co Ltd
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Priority to CN201410756372.7A priority Critical patent/CN104429612A/en
Publication of CN104429612A publication Critical patent/CN104429612A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity

Abstract

The invention discloses a bottle cultivation method for ombre mushroom. The processes include preparing ombre mushroom cultivation medium, putting into a bottle, autoclaving, inoculation and the like, and further include picking up of the ombre mushroom and removing of mushroom roots. All the processes are executed by machines, both labors and energy are saved, and is benefit for the expanding of production scale and the increasing of yield. Meanwhile high and stable yield is realized and good social benefit is achieved. The water-retaining property of cultivation bottles is much better than that of cultivation bags. The flushing time of each batch is cut down by 3 to 5 days, the time of occupying mushroom houses is greatly saved, and the utilization rate of mushroom houses is increased. Meanwhile, the hypha of the cultivation medium is rapid in growing speed, high in biotransformation rate, high in output and low in manufacture cost.

Description

Phoenix-tail mushroom breeding method planted by a kind of bottle
Technical field
The present invention relates to bacterium mushroom cultivation field, be specifically related to a kind of bottle and plant phoenix-tail mushroom breeding method.
Background technology
Phoenix-tail mushroom, or claim handle bucket mushroom, belong to Basidiomycetes, Agaricales, Pleurotaceae, Pleurotus; Phoenix-tail mushroom cap is canescence, and likeness in form phoenix tail, originates in knob, the Himalayas.Within 1979, introduce Hong Kong from India to cultivate successfully.Introduce by Guangdong and plant experimentally, progressively promote the whole nation.Phoenix-tail mushroom quality is tender and crisp, fresh and sweet tasty and refreshing, nutritious, is rich in each seed amino acid of protein, polysaccharide, vitamin, trace element and needed by human body, quite by consumers.
Although the cultivation of current phoenix-tail mushroom is widelyd popularize in a lot of area, most is bag cultivation, and the defect that bag is planted is that moisture content nutrient is easily lost, and is not easy to adopt machine operation, and cost of labor is large.
Summary of the invention
For solving the problem, the invention provides a kind of bottle and planting phoenix-tail mushroom breeding method.
For achieving the above object, the technical scheme that the present invention takes is:
Phoenix-tail mushroom breeding method planted by a kind of bottle, comprises the steps:
S1, get corncob 36-48 weight portion, potato residues 8-10 weight portion, sweet potato vine 18-22 weight portion, phosphate fertilizer 0.5-0.9 part, calcium carbonate fiber 2-3 weight portion, sucrose 1-2 weight portion, carbendazim 0.5-1.5 weight portion, urea 0.2-0.4 weight portion pulverize respectively, its particle size is at below 5mm, and grain thickness degree is even;
S2, by raw material pulverize after mixing and stirring after add water, make its water content reach 50%-70%, stir 30 minutes, pH controls at 7.0-7.5;
Rear heap fermentation mixed thoroughly by S3, raw material, make high 1.1-1.2 rice, trapezoidal heap that bottom width 1.4-1.5 rice, length are not limit, beat some ventilation holes with shovel handle thereon, fermentation 10-12 days,, add appropriate water again after fermentation ends, mix thoroughly, regulate water content between 65-73%, then edible mushroom bag is loaded, every bag of folding dress siccative 400-600 gram, degree of tightness wants appropriateness, tightens two ends sack with cord;
First temperature is risen to 100-105 DEG C under S4, normal pressure, insulation 20-25min; Then temperature is risen to 110-115 DEG C, insulation 60-70min; Again temperature is risen to 120-125 DEG C, 60-70min is incubated when pressure is 0.13-0.14MPa, last vexedly again put 30-35min, stop heating, allow it naturally cool to less than 28 DEG C, take out bottling, wherein bottle needs the air-vent got at the bottom of multiple straight-through bottle namely to inoculate cave, is convenient at the bottom of bacterial classification access bottle;
S5, connect in every bottle of 1400mL medium 35-50mL liquid spawn ratio inoculation, inoculation temperature controls at 20 DEG C-25 DEG C; Lucifuge is cultivated, and inoculates mycelia after 10-15 days and can stretch into medium 20-25mm, and the part that mycelia spreads becomes white, can see at the bottom of bottle and to grow to inoculate centered by cave, inoculate full bottle after about 35 days and all become white, mycelia covers whole charge level, forms the first damp mushroom mycelia;
S6, cobalt-60γray process is adopted to the bacterial classification cultivated through 35 days after, open bottle cap culture bottle is sidelong cultivates, daytime, room temperature remained on 15-22 DEG C, day and night temperature controls at 5-8 DEG C, gives 500-1000lx scattered light every day, keeps 3-5 days continuously, Deng mushroom handle extend reach 3-4cm, bacteria cover diameter reach 2cm time, adopt thin sprayer to spare no effort to spraying, can adopt mushroom after 1 day, this is the first damp mushroom;
S7, after the first damp mushroom adopts mushroom mycelium stimulation, the humidity in mushroom room maintains 60-70%, and now the damp mushroom mycelia of charge level second recovers gradually, bacteria 5-7 days; When charge level mycelia recover completely dense white after, completely drenched to charge level to charge level water spray, storehouse temperature drop to 10 DEG C is carried out low temperature stimulation 24 hours simultaneously, keep space humidity 80%-90% simultaneously, and increase light application time and the number of times in mushroom room, after mushroom flower bud manifests again, its management process is with the first damp mushroom;
S8, after having adopted the second damp mushroom, moved down from cultivating stand by culture bottle, dug out by composts or fertilisers of cultivating, culture bottle has recycled.
As preferably, the preparation method of described calcium carbonate fiber is: make fine slaked lime high-speed motion under leather hard by high-speed air dispersion machine propeller accelecrated motion, and fully contact with carbonic acid gas, the Ca2+ of ionization generates CaCO3 with the CO2 fast reaction passed into and precipitates, spray the magnesium chloride solution containing free Mg2+ simultaneously, make Mg2+ be attached to calcium carbonate crystal surface, thus weaken the growth rate of calcium carbonate crystal face, and side direction quick links, the fibrous calcium carbonate fiber of final formation.
The present invention has following beneficial effect:
The operation such as preparation, bottling, autoclaving, inoculation of phoenix-tail mushroom composts or fertilisers of cultivating of the present invention, and the harvesting of phoenix-tail mushroom and go the work of mushroom pin, all adopt machine operation, save labor, be conducive to expanding the scale of production, increase yield, reaches high and stable yields to obtain better social benefit simultaneously; The moisture capacity of culture bottle is much better than cultivation bag, the change of tide time shorten 3-5 days often criticized, and greatly save and account for the mushroom room time, improve mushroom room availability, composts or fertilisers of cultivating mycelial growth rate of the present invention is fast simultaneously, biological transformation ratio and output high, production cost is low.
Embodiment
In order to make objects and advantages of the present invention clearly understand, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
The preparation method of the calcium carbonate fiber that following examples use is: make fine slaked lime high-speed motion under leather hard by high-speed air dispersion machine propeller accelecrated motion, and fully contact with carbonic acid gas, the Ca2+ of ionization generates CaCO3 with the CO2 fast reaction passed into and precipitates, spray the magnesium chloride solution containing free Mg2+ simultaneously, Mg2+ is made to be attached to calcium carbonate crystal surface, thus weaken the growth rate of calcium carbonate crystal face, and side direction quick links, the fibrous calcium carbonate fiber of final formation.
Embodiment 1
Phoenix-tail mushroom breeding method planted by a kind of bottle, comprises the steps
S1, get corncob 36 weight portion, potato residues 10 weight portion, sweet potato vine 22 weight portion, 0.9 part, phosphate fertilizer, calcium carbonate fiber 3 weight portion, sucrose 2 weight portion, carbendazim 1.5 weight portion, urea 0.4 weight portion branch pulverize, its particle size is at below 5mm, and grain thickness degree is even;
S2, by raw material pulverize after mixing and stirring after add water, make its water content reach 50%, stir 30 minutes, pH controls 7.0;
Rear heap fermentation mixed thoroughly by S3, raw material, make the trapezoidal heap that high 1.1 meters, bottom width 1.4 meters, length are not limit, beat some ventilation holes with shovel handle thereon, ferment 10 days,, add appropriate water again after fermentation ends, mix thoroughly, regulate water content between 65%, then edible mushroom bag is loaded, every bag of folding dress siccative 400-600 gram, degree of tightness wants appropriateness, tightens two ends sack with cord;
First temperature is risen to 100 DEG C under S4, normal pressure, insulation 20min; Then temperature is risen to 110 DEG C, insulation 60min; Again temperature is risen to 120 DEG C, be incubated 60min when pressure is 0.13MPa, finally vexedly again put 30min, stop heating, allow it naturally cool to less than 28 DEG C, take out bottling, wherein bottle needs the air-vent got at the bottom of multiple straight-through bottle namely to inoculate cave, is convenient at the bottom of bacterial classification access bottle;
S5, connect in every bottle of 1400mL medium 35mL liquid spawn ratio inoculation, inoculation temperature controls at 20 DEG C; Lucifuge is cultivated, and inoculate mycelia after 10 days and can stretch into medium 20mm, and part that mycelia spreads becomes white, can see growing to inoculate centered by cave at the bottom of bottle, and inoculate full bottles after about 35 days and all become white, mycelia covers whole charge level, formation the first damp mushroom mycelia;
S6, cobalt-60γray process is adopted to the bacterial classification cultivated through 35 days after, open bottle cap culture bottle is sidelong cultivates, daytime, room temperature remained on 15 DEG C, day and night temperature controls, at 8 DEG C, to give 1000lx scattered light every day, keeps 3 days continuously, Deng mushroom handle extend reach 3cm, bacteria cover diameter reach 2cm time, adopt thin sprayer to spare no effort to spraying, can adopt mushroom after 1 day, this is the first damp mushroom;
S7, after the first damp mushroom adopts mushroom mycelium stimulation, the humidity in mushroom room maintains 70%, and now the damp mushroom mycelia of charge level second recovers gradually, bacteria 5 days; When charge level mycelia recover completely dense white after, completely drenched to charge level to charge level water spray, storehouse temperature drop to 10 DEG C is carried out low temperature stimulation 24 hours simultaneously, keep space humidity 80%-90% simultaneously, and increase light application time and the number of times in mushroom room, after mushroom flower bud manifests again, its management process is with the first damp mushroom;
S8, after having adopted the second damp mushroom, moved down from cultivating stand by culture bottle, dug out by composts or fertilisers of cultivating, culture bottle has recycled.
Embodiment 2
Phoenix-tail mushroom breeding method planted by a kind of bottle, it is characterized in that, comprises the steps
S1, get corncob 42 weight portion, potato residues 9 weight portion, sweet potato vine 20 weight portion, 0.7 part, phosphate fertilizer, calcium carbonate fiber 2.5 weight portion, sucrose 1.5 weight portion, carbendazim 1 weight portion, urea 0.3 weight portion pulverize respectively, its particle size is at below 5mm, and grain thickness degree is even;
S2, by raw material pulverize after mixing and stirring after add water, make its water content reach 60%, stir 30 minutes, pH controls 7.25;
Rear heap fermentation mixed thoroughly by S3, raw material, make the trapezoidal heap that high 1.15 meters, bottom width 1.45 meters, length are not limit, beat some ventilation holes with shovel handle thereon, ferment 11 days,, add appropriate water again after fermentation ends, mix thoroughly, regulate water content between 69%, then edible mushroom bag is loaded, every bag of folding dress siccative 400-600 gram, degree of tightness wants appropriateness, tightens two ends sack with cord;
First temperature is risen to 102.5 DEG C under S4, normal pressure, insulation 22.5min; Then temperature is risen to 112.5 DEG C, insulation 65min; Again temperature is risen to 122.5 DEG C, be incubated 65min when pressure is 0.135MPa, finally vexedly again put 32.5min, stop heating, allow it naturally cool to less than 28 DEG C, take out bottling, wherein bottle needs the air-vent got at the bottom of multiple straight-through bottle namely to inoculate cave, is convenient at the bottom of bacterial classification access bottle;
S5, connect in every bottle of 1400mL medium 42.5mL liquid spawn ratio inoculation, inoculation temperature controls at 22.5 DEG C; Lucifuge is cultivated, and inoculates mycelia after 12.5 days and can stretch into medium 22.5mm, and the part that mycelia spreads becomes white, can see at the bottom of bottle and to grow to inoculate centered by cave, inoculate full bottle after about 35 days and all become white, mycelia covers whole charge level, forms the first damp mushroom mycelia;
S6, cobalt-60γray process is adopted to the bacterial classification cultivated through 35 days after, open bottle cap culture bottle is sidelong cultivates, daytime, room temperature remained on 18.5 DEG C, day and night temperature controls, at 6.5 DEG C, to give 750lx scattered light every day, keeps 4 days continuously, Deng mushroom handle extend reach 3.5cm, bacteria cover diameter reach 2cm time, adopt thin sprayer to spare no effort to spraying, can adopt mushroom after 1 day, this is the first damp mushroom;
S7, after the first damp mushroom adopts mushroom mycelium stimulation, the humidity in mushroom room maintains 65%, and now the damp mushroom mycelia of charge level second recovers gradually, bacteria 6 days; When charge level mycelia recover completely dense white after, completely drenched to charge level to charge level water spray, storehouse temperature drop to 10 DEG C is carried out low temperature stimulation 24 hours simultaneously, keep space humidity 85% simultaneously, and increase light application time and the number of times in mushroom room, after mushroom flower bud manifests again, its management process is with the first damp mushroom;
S8, after having adopted the second damp mushroom, moved down from cultivating stand by culture bottle, dug out by composts or fertilisers of cultivating, culture bottle has recycled.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.

Claims (2)

1. a phoenix-tail mushroom breeding method planted by bottle, it is characterized in that, comprises the steps:
S1, get corncob 36-48 weight portion, potato residues 8-10 weight portion, sweet potato vine 18-22 weight portion, phosphate fertilizer 0.5-0.9 part, calcium carbonate fiber 2-3 weight portion, sucrose 1-2 weight portion, carbendazim 0.5-1.5 weight portion, urea 0.2-0.4 weight portion pulverize respectively, its particle size is at below 5mm, and grain thickness degree is even;
S2, by raw material pulverize after mixing and stirring after add water, make its water content reach 50%-70%, stir 30 minutes, pH controls at 7.0-7.5;
Rear heap fermentation mixed thoroughly by S3, raw material, make high 1.1-1.2 rice, trapezoidal heap that bottom width 1.4-1.5 rice, length are not limit, beat some ventilation holes with shovel handle thereon, fermentation 10-12 days,, add appropriate water again after fermentation ends, mix thoroughly, regulate water content between 65-73%, then edible mushroom bag is loaded, every bag of folding dress siccative 400-600 gram, degree of tightness wants appropriateness, tightens two ends sack with cord;
First temperature is risen to 100-105 DEG C under S4, normal pressure, insulation 20-25min; Then temperature is risen to 110-115 DEG C, insulation 60-70min; Again temperature is risen to 120-125 DEG C, 60-70min is incubated when pressure is 0.13-0.14MPa, last vexedly again put 30-35min, stop heating, it is allowed to naturally cool to less than 28 DEG C, take out bottling, wherein bottle needs the air-vent got at the bottom of multiple straight-through bottle namely to inoculate cave, is convenient at the bottom of bacterial classification access bottle;
S5, connect in every bottle of 1400mL medium 35-50mL liquid spawn ratio inoculation, inoculation temperature controls at 20 DEG C-25 DEG C; Lucifuge is cultivated, and inoculates mycelia after 10-15 days and can stretch into medium 20-25mm, and the part that mycelia spreads becomes white, can see at the bottom of bottle and to grow to inoculate centered by cave, inoculate full bottle after about 35 days and all become white, mycelia covers whole charge level, forms the first damp mushroom mycelia;
S6, cobalt-60γray process is adopted to the bacterial classification cultivated through 35 days after, open bottle cap culture bottle is sidelong cultivates, daytime, room temperature remained on 15-22 DEG C, day and night temperature controls at 5-8 DEG C, gives 500-10001x scattered light every day, keeps 3-5 days continuously, Deng mushroom handle extend reach 3-4cm, bacteria cover diameter reach 2cm time, adopt thin sprayer to spare no effort to spraying, can adopt mushroom after 1 day, this is the first damp mushroom;
S7, after the first damp mushroom adopts mushroom mycelium stimulation, the humidity in mushroom room maintains 60-70%, and now the damp mushroom mycelia of charge level second recovers gradually, bacteria 5-7 days; When charge level mycelia recover completely dense white after, completely drenched to charge level to charge level water spray, storehouse temperature drop to 10 DEG C is carried out low temperature stimulation 24 hours simultaneously, keep space humidity 80%-90% simultaneously, and increase light application time and the number of times in mushroom room, after mushroom flower bud manifests again, its management process is with the first damp mushroom;
S8, after having adopted the second damp mushroom, moved down from cultivating stand by culture bottle, dug out by composts or fertilisers of cultivating, culture bottle has recycled.
2. phoenix-tail mushroom breeding method planted by a kind of bottle according to claim 1, it is characterized in that, the preparation method of described calcium carbonate fiber is: make fine slaked lime high-speed motion under leather hard by high-speed air dispersion machine propeller accelecrated motion, and fully contact with carbonic acid gas, the Ca2+ of ionization generates CaCO3 with the CO2 fast reaction passed into and precipitates, spray the magnesium chloride solution containing free Mg2+ simultaneously, Mg2+ is made to be attached to calcium carbonate crystal surface, thus weaken the growth rate of calcium carbonate crystal face, and side direction quick links, the fibrous calcium carbonate fiber of final formation.
CN201410756372.7A 2014-12-12 2014-12-12 Bottle cultivation method for ombre mushroom Pending CN104429612A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105165401A (en) * 2015-09-12 2015-12-23 辽东学院 Cordyceps militaris liquid strain stabilizing technology
CN105557299A (en) * 2015-12-05 2016-05-11 刘晓红 Wild tricholoma matsutake artificial domestication method
CN106036446A (en) * 2016-07-01 2016-10-26 河北大学 Pleurotus pulmonarius flavored potato and jujube steamed sponge cakes and preparation method thereof
CN108718921A (en) * 2018-09-07 2018-11-02 界首市万花巢生物科技有限公司 A kind of cultural method of Se-rich xianggu
CN108934767A (en) * 2018-09-07 2018-12-07 界首市万花巢生物科技有限公司 A kind of cultural method improving edible mushroom quality

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105165401A (en) * 2015-09-12 2015-12-23 辽东学院 Cordyceps militaris liquid strain stabilizing technology
CN105557299A (en) * 2015-12-05 2016-05-11 刘晓红 Wild tricholoma matsutake artificial domestication method
CN106036446A (en) * 2016-07-01 2016-10-26 河北大学 Pleurotus pulmonarius flavored potato and jujube steamed sponge cakes and preparation method thereof
CN108718921A (en) * 2018-09-07 2018-11-02 界首市万花巢生物科技有限公司 A kind of cultural method of Se-rich xianggu
CN108934767A (en) * 2018-09-07 2018-12-07 界首市万花巢生物科技有限公司 A kind of cultural method improving edible mushroom quality

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