CN104381598A - Method for biologically optimizing nutritional value of grape seeds - Google Patents
Method for biologically optimizing nutritional value of grape seeds Download PDFInfo
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- CN104381598A CN104381598A CN201410562979.1A CN201410562979A CN104381598A CN 104381598 A CN104381598 A CN 104381598A CN 201410562979 A CN201410562979 A CN 201410562979A CN 104381598 A CN104381598 A CN 104381598A
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Abstract
The invention relates to a method for biologically optimizing nutritional value of grape seeds. Grape seed meal is fermented by a composite microbial method, and the best nitrogen source, best inorganic salt and best addition proportion in the process of fermenting the grape seed meal feed by composite microbial method are determined through screening of single-factor test. The method comprises the following steps: preparing a grape seed meal fermentation culture medium, preparing a fermentation strain amplification culture medium, amplifying fermentation strain and inoculating. Compared with the non-fermented mixture substrate, the content of crude protein in fermentation products can be obviously increased by 59.28%, the content of crude fiber is reduced by 29.16%, and the nutrition utilization value of the grape seed meal is greatly improved; the production performance of livestock and poultry feed can be obviously improved through using the fermentation products, the cost of feed is reduced, and the purpose of more environment protection and high efficiency is achieved. The technology can be widely applied in further development of grape seed meal products and suitable for different feeds for livestock and poultry, and has wide market development prospect.
Description
Technical field
The invention belongs to fermentative feedstuff of microbe technical field, be specifically related to a kind of method of Biological optimization grape pip dregs of rice nutritive value.
Background technology
According to statistics, the whole world year malaga about 100,000,000 tons, China's year malaga about 1,000 ten thousand tons, but also increasing year by year.Grape pip is the accessory substance produced in Grape processing process, accounts for 4% ~ 6% of whole grain grape weight.Grape pip is rich in a large amount of polyphenols, linoleic acid, amino acid, multivitamin, crude fat, trace element; Wherein, polyphenols procyanidins (OPC) is main matter, has superpower oxidation resistance, is 50 times of vitamin E, is ascorbic 20 times; It has removing interior free yl, the effect of oxidation resistance in reinforcement, and therefore, grape pip is used as extracting grape-kernel oil more.The grape pip dregs of rice typically refer to grape wine factory fermentation produce leftover bits and pieces or crushing grapes seed oil after byproduct, crude fiber content is up to 50.81%, and crude protein 11.52%, calcium 0.80%, phosphorus 0.28%, content of lignin is 14.98%.Because grape pip dregs of rice crude fibre is low containing height, protein content, and containing ANFs such as tannin, nutritional utilization is worth lower, does not have the method for scientific utilization at present, causes the serious waste of resource.
Along with China's livestock and poultry cultivation scale constantly increases, feedstuff resource more and more lacks.At present, when the conventional feed such as corn and dregs of beans and processed side product price thereof constantly rise, livestock and poultry of being fed by the grape pip dregs of rice products substitution part conventional feed raw material after processing process, have become the focus that Animal nutrition scholars pay close attention to.Therefore, many Feed Manufacturing producers all exploring new feed resource approach, expect the grape pip dregs of rice to add in livestock and poultry diet, reach and save corn, the raising production performance of livestock and poultry and the object of economic benefit.Up to now, systematic research is not carried out for feeding grape pip dregs of rice processing and utilization both at home and abroad, optimize grape pip dregs of rice nutritive value fermentation technique and be also in blank.For this reason, further investigation best complex flora fermented grape seed dregs of rice technology, is worth improving its nutritional utilization further, expands its range of application in feed, has important economy and social value.
Summary of the invention
The object of this invention is to provide a kind of method of Biological optimization grape pip dregs of rice nutritive value, namely adopt microbial composite bacteria group to carry out the fermented grape seed dregs of rice, to fill up the blank of this art at present.
Applicant, by research for a long time, through the screening of various factors, determines the optimum process condition of microorganism combining bacteria mass-sending ferment grape pip dregs of rice feed.
The fermentation process of the grape pip dregs of rice of the present invention, specifically comprises the steps:
1) raw material disposal: it is that 40 order pulverizers are pulverized that the grape pip dregs of rice and wheat bran are mixed rear granularity; Then be added to the water and make culture medium;
2) preparation of multiple and flora: make composite flora after penicillium oxalicum, lactic acid bacteria, bacillus subtilis and S. cervisiae being carried out expansion cultivation;
3) composite flora prepared is joined the fermentation carrying out the grape pip dregs of rice in the grape pip dregs of rice after pulverizing and wheat bran; The temperature of fermentation is preferably 30 DEG C, and fermentation time is preferably 72h.
Described step 2) in composite flora, wherein penicillium oxalicum, lactic acid bacteria, bacillus subtilis, S. cervisiae, the number ratio of its bacterial strain is 3 × 10
7: 1 × 10
7: 2 × 10
7: 1 × 10
7;
Described step 1) in culture medium in the mass ratio of the grape pip dregs of rice and wheat bran be 4:1; And the grape pip dregs of rice and wheat bran concentration is in the medium 85%;
Nitrogenous source and inorganic salts are also added with in culture medium; Described nitrogenous source is ammonium nitrate, and its addition is in the medium 2.0%;
Described inorganic salts are sodium hydrogen phosphate and calcium carbonate, and its addition is respectively 0.2% and 0.1%;
First method of the present invention has prepared a kind of complex microbial community, effectively can be optimized fermentation to grape pip dregs of rice nutritive value; In addition, the culture medium of the present invention to fermentation has carried out formula combination; Add ammonium nitrate as nitrogenous source, interpolation sodium hydrogen phosphate and calcium carbonate are as inorganic salts; The growth of bacterial classification can be promoted by interpolation nitrogenous source and inorganic salts, thus improve the protein content of fermented feed and reduce content of cellulose.Its ferment effect is better than the fermentation not adding nitrogenous source and inorganic salts.And, the technique of this invention effectively can increase production efficiency, improve the quality of products and palatability, add Product Activity content of material and nutritional utilization value, meet nowadays feedstuff industry cheapness and demand for security, the scientific utilization for the grape pip dregs of rice provides the method for science more.
Accompanying drawing explanation
Fig. 1: the graph of a relation of the content of nitrogenous source addition and the true albumen of tunning;
Fig. 2: the graph of a relation of the content of inorganic salts addition and the true albumen of tunning;
Fig. 3: the graph of a relation of fermentation temperature and tunning crude protein content;
Fig. 4: the graph of a relation of fermentation time and tunning crude protein content;
Fig. 5: the graph of a relation of material-water ratio and tunning crude protein content;
Fig. 6: the graph of a relation of thickness of feed layer and tunning crude protein content.
detailed description of the invention
Applicant considers the problems such as environmental protection, energy-conservation and cost, adopt the microbe fermentation method process grape pip dregs of rice, the product protein content produced is high, ANFs content is few, be rich in beneficial bacterium, and environmental pollution is little, meets the strategy of China's joint grain, environmental protection and sound development.
Below in conjunction with embodiment, method of the present invention is described in detail.
Preparation method of the present invention, comprises following step:
1) raw material disposal:
The grape pip dregs of rice and wheat bran pulverizer are pulverized, granularity is 40 orders, be 4:1 in the ratio of the grape pip dregs of rice and wheat bran, material-water ratio is that 2:1 is mixed to join in 250ml triangular flask, grape pip dregs of rice 40g is added, wheat bran 10g, distilled water 25ml in each triangular flask, add cotton plug, with pressure cooker 121 DEG C of sterilizing 30min.
2) preparation of composite flora:
The present invention have passed through and long-term determined a kind of strain combination for the fermented grape seed dregs of rice, and its composite fermentation bacterial strain is made up of F67, lactic acid bacteria, bacillus subtilis and S. cervisiae.
Above-mentioned four kinds of fermented bacterium composite fermentations that the present invention selects, wherein the cellulose degradation in the grape pip dregs of rice can be that monose utilizes for other three bacterial classifications by F67, simultaneously by grape pip dregs of rice cell wall degradation, intracellular nutritional material is separated out, provide condition for follow-up bacterial classification utilizes; Bacillus subtilis can be degraded ANFs (tannin) main in the grape pip dregs of rice, has fragrant simultaneously, gives the taste that product is special; The monose that S. cervisiae can utilize F67 to produce grows fast, and then improves microprotein content in product; The growth of above three bacterial classifications can consume a large amount of oxygen, maintains anaerobic environment in sweat and then provides condition for lactobacter growth; Lactic acid bacteria produces lactic acid in growth course, cooperatively gives grape pip dregs of rice product clear sour fragrance with bacillus subtilis.Above four bacterial classifications are mutually promoted, again mutual antagonism, Application of composite, have the nutrition content increasing the grape pip dregs of rice, the prebiotic effect improved in animal palatability and nutrient utilization, grape pip dregs of rice poisonous and harmful substance of degrading and good animal productiong.
The present invention lactic acid bacteria used is lactobacterium acidophilus, its strain number is ACCC10637, bacillus subtilis be numbered CICC20506, S. cervisiae be numbered CICC1012.But under invention thinking of the present invention, still can be tried other bacterial strain by test.For F67 (penicillium oxalicum), this bacterium to be separated by high-quality aquatic bird research institute of Qingdao Agricultural University and to obtain from goose enteron aisle, have the very high degradation capability of cellulose, identify through Institute of Microorganism, Academia Sinica and be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (microbial preservation numbering: CGMCC No.2260), and confirm this bacterium safety non-toxic through qualification mechanism.Above-mentioned 4 kinds of bacterial classification prescriptions use can protein content in the maximum raising grape pip dregs of rice and reduce content of cellulose, and can degradable Main Antinutritional Factors tannin.
3) preparation of fermented bacterium amplification culture medium:
F67 amplification culture medium compound method: adding distil water 1000ml in beaker, adds peptone 5.0g successively, dipotassium hydrogen phosphate 1.0g, yeast extract powder 2.0g, magnesium sulfate 0.5g, tepor is dissolved, regulate pH value to be about 6.8, boil, after adding glucose 20.0g dissolving, shake up, gauze filtering, is 6.4 ± 0.2 after regulating pH value to make sterilizing, is divided in in each triangular flask, add cotton plug, use pressure cooker sterilizing.Cultivation temperature is 30 DEG C, and incubation time is 18h ~ 24h, cultivates after terminating, and takes out to be placed in the refrigerator of 4 DEG C and preserve from shaking table.
Lactic acid bacteria amplification culture medium compound method: adding distil water 1000ml in beaker, add peptone 10.0g successively, beef leaching thing (beef extract) 10.0g, yeast extract (yeast extract or dusty yeast) 5.0g, glucose 20.0g, sodium acetate 5.0g, citric acid hydrogen diamine 2.0g, Tween-80 1.0ml, dipotassium hydrogen phosphate 2.0g, epsom salt 0.2g, seven water manganese sulfate 0.05g, regulate pH6.2 ~ 6.4 with 10% hydrochloric acid, are divided in in each triangular flask, add cotton plug, use pressure cooker sterilizing.After inoculation, cultivation temperature is 42 DEG C, amphimicrobian, and cultivation temperature is 18h ~ 20h, cultivates after terminating, and takes out to be placed in the refrigerator of 4 DEG C and preserve from incubator.
Bacillus subtilis amplification culture medium compound method: adding distil water 1000ml in beaker, adds sucrose 10g, soy peptone 10g, NaCl 5g successively, is placed on the upper heating of fire.Until completely dissolved, with 10% hydrochloric acid or 10% NaOH adjusted to ph to 7.2 ~ 7.6, be divided in in each triangular flask, add cotton plug, use pressure cooker sterilizing.After inoculation, cultivation temperature is 37 DEG C, and incubation time is 18h ~ 24h, cultivates after terminating, and takes out to be placed in the refrigerator of 4 DEG C and preserve from shaking table.
S. cervisiae amplification culture medium compound method: get 250g dried malt, ground by dried malt, granularity is 100 orders, puts into large beaker, adds 1000ml distilled water, saccharification 3h ~ 4h in 64 DEG C ~ 66 DEG C water-baths.By saccharified liquid 4 ~ 6 layers of filtered through gauze, if filtrate is muddy, clarify with Egg-white.Method is, is taken out by the albumen in an egg and to add water 20ml, mix well till being stirred to raw foam with glass bar, then pours in saccharified liquid after stirring and boiling, by 8 layers of filtered through gauze.Filtrate regulates PH to be 7.0 ~ 7.2, and pol saccharometer is measured and adding distil water is diluted to 5 ° of Be, is divided in in each triangular flask, adds cotton plug, use pressure cooker sterilizing.Cultivation temperature is 25 DEG C ~ 28 DEG C, and incubation time is 24h, cultivates after terminating, and takes out to be placed in the refrigerator of 4 DEG C and preserve from shaking table.
4) grape pip dregs of rice technique fermentation:
Using the grape pip dregs of rice after pulverizing and wheat bran as the culture medium of Major Nutrient material.The F67 increased, lactic acid bacteria, bacillus subtilis and S. cervisiae three kinds of bacterium liquid are diluted to 1 × 10 with sterilized water respectively
7individual/ml, is inoculated on fermentation medium, adds 2.0% ammonium nitrate as nitrogenous source in culture medium; Add 0.2% sodium hydrogen phosphate and 0.1% calcium carbonate as inorganic salts simultaneously; Above-mentioned each bottle of culture medium is inoculated the bacterium liquid of 7.5ml.Add cotton plug after having inoculated, triangle bottleneck brown paper encases, and is placed in the constant incubator of 30 DEG C and cultivates, and incubation time is that 72h completes fermentation.
Embodiment 1: the screening test of composite bacteria Biological optimization grape pip dregs of rice nitrogenous source and inorganic salts
1) raw material disposal:
The grape pip dregs of rice and wheat bran pulverizer are pulverized, granularity is 40 orders, be 4:1 in the ratio of the grape pip dregs of rice and wheat bran, material-water ratio is that 2:1 is mixed to join in 250ml triangular flask, grape pip dregs of rice 40g is added, wheat bran 10g, distilled water 25ml in each triangular flask, add cotton plug, with pressure cooker 121 DEG C of sterilizing 30min.
The F67 increased, lactic acid bacteria, bacillus subtilis, S. cervisiae bacterium liquid are diluted to 1 × 10 with sterilized water respectively
7individual/ml, is inoculated on fermentation medium, adds urea, ammonium chloride, ammonium nitrate, ammonium oxalate respectively as nitrogenous source in each culture medium, and its addition is 1.0%, 1.5%, 2.0%, 2.5%, and its adding proportion is 0.5g, 0.75g, 1.0g, 1.25g.Each culture medium is inoculated respectively the bacterium liquid of 7.5ml.Add cotton plug after having inoculated, triangle bottleneck brown paper encases, and is placed in the constant incubator of 30 DEG C and cultivates, and incubation time is 72h.Protein content in tunning is measured with reference to (GB/T 6432-1994); The crude fiber content in tunning is measured with reference to (GB/T6434-1994); Tannin content in tunning is measured with reference to (GB/T 27985-2011).
Result of the test shows, in solid state fermentation test, these four kinds of nitrogenous sources of urea, ammonium chloride, ammonium oxalate, ammonium nitrate add the content that all can improve the true albumen of tunning; But when waiting nitrogen quantity, ammonium nitrate to the amplification of the true protein content of tunning higher than other nitrogenous source; When ammonium nitrate addition is 2.0%, the true protein content of tunning is comparatively large, and improve 83.59% respectively than unfermentable substrate, crude fibre is compared and reduced 28.23%.Find in addition, mixed bacteria all almost can not utilize urea, and the true protein content of tunning does not significantly increase, and can not increase with the increase of urea addition, illustrates that hybrid bacterial strain can not utilize urea as nitrogenous source substantially.Consider above situation, determine that in the fermentation of the grape pip dregs of rice, more suitable nitrogenous source is ammonium nitrate, its addition is 2.0%.See accompanying drawing 1.
2) inorganic salts screening test:
The F67 increased, lactic acid bacteria, bacillus subtilis, S. cervisiae bacterium liquid are diluted to 1 × 10 with sterilized water respectively
7individual/ml, be inoculated on fermentation medium, adding sodium hydrogen phosphate, sodium dihydrogen phosphate, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, calcium chloride, calcium carbonate in each culture medium is respectively 0.1%, 0.2%, 0.3% (and being 0.05g, 0.1g, 0.15g) as its addition of inorganic salts.Add cotton plug after having inoculated, triangle bottleneck brown paper encases, and is placed in the constant incubator of 30 DEG C and cultivates, and incubation time is 72h.
Result of the test shows, the Na of 0.2%
2hPO
4additive effect best, CP content compared with other group content the highest, improve 52.90% than unfermentable mixed substrates, crude fibre reduces 28.77%.The interpolation of Ca can improve the CP of tunning equally, and calcium carbonate effect and cost are all better than calcium chloride, and when addition is 0.1%, CP content is the highest compared with other group content, and improve 35.50% than unfermentable mixed substrates, crude fibre reduces 24.30%.Consider above situation, determine that in the fermentation of the grape pip dregs of rice, more suitable inorganic salts are the Na of 0.2%
2hPO
4with 0.1% calcium carbonate.See accompanying drawing 2.
Embodiment 2: grape pip dregs of rice feed fermenting process conditional filtering is tested
1) raw material disposal:
The grape pip dregs of rice and wheat bran pulverizer are pulverized, granularity is 40 orders, be 4:1 in the ratio of the grape pip dregs of rice and wheat bran, material-water ratio is that 2:1 is mixed to join in 250ml triangular flask, adds grape pip dregs of rice 40g in each triangular flask, wheat bran 10g, distilled water 25ml, ammonium nitrate 1.0g, sodium hydrogen phosphate 0.1g, calcium carbonate 0.05g, add cotton plug, with pressure cooker 121 DEG C of sterilizing 30min.
2) selection of optimum fermentation temp:
After having inoculated, add cotton plug after having inoculated, triangle bottleneck brown paper encases, and cultivation be placed in the constant incubator of 25 DEG C, 30 DEG C, 35 DEG C, 40 DEG C, 45 DEG C respectively and cultivate, incubation time is 72h
Result of the test shows, at the beginning along with the rising of temperature, tunning crude protein content raises gradually, and reach the unfermentable mixed substrates of high specific at 30 DEG C and improve 47.56%, crude fibre reduces 25.21%.But continuation raised temperature, inhibits part microbial growth on the contrary, makes crude protein content decline to some extent on the contrary.Therefore employing 30 DEG C is comparatively suitable aborning.See accompanying drawing 3.
3) determination of best fermentation time:
Cotton plug is added after having inoculated, triangle bottleneck brown paper encases, cultivation be placed in the constant incubator of 30 DEG C and cultivate, test is chosen incubation time and is respectively 24h, 36h, 48h, the tunning of 60h, 72h, 84h, its CP, NDF, ADF and CF content is measured, thus determines the suitableeest fermentation time.
Result of the test shows, along with the growth of fermentation time, tunning CP content presents the trend of falling after rising, and fermentation 72h tunning crude protein content reaches the highest, and improve 49.04% than unfermentable mixed substrates, crude fibre reduces 25.88%.Therefore fermentation time is 72h comparatively suitable (Fig. 4) aborning.
4) determination of best material-water ratio:
The grape pip dregs of rice and wheat bran pulverizer are pulverized, granularity is 40 orders, be 4:1 in the ratio of the grape pip dregs of rice and wheat bran, material-water ratio is regulated to be that 1:1,1:2,1:3,1:4,1:5 (and adding distil water is 25ml, 16.66ml, 12.5ml, 10ml, 8.33ml respectively) are mixed to join in 250ml triangular flask, grape pip dregs of rice 40g is added, wheat bran 10g, calcium carbonate 0.05g, ammonium nitrate 1.0g, sodium hydrogen phosphate 0.1g in each triangular flask, add cotton plug, with pressure cooker 121 DEG C of sterilizing 30min.By weight 15% by four strain inoculation on culture medium, after having inoculated, be put into by culture medium in incubator, cultivation temperature is 30 DEG C, and incubation time is 72h.
Result of the test shows, along with the increase of material-water ratio, CP content first increases rear reduction, NDF, ADF and CF content first reduces rear increase, when material-water ratio is 2:1, crude protein content in tunning reaches the highest, and improve 48.26% than unfermentable mixed substrates, crude fibre reduces 26.29%.Therefore material-water ratio should be 2:1 aborning.See accompanying drawing 5.
5) determination of best thickness of feed layer:
The grape pip dregs of rice and wheat bran pulverizer are pulverized, granularity is 40 orders, be 4:1 in the ratio of the grape pip dregs of rice and wheat bran, material-water ratio is that 2:1 is mixed to join in 250ml triangular flask, adds grape pip dregs of rice 40g, wheat bran 10g in each triangular flask, calcium carbonate 0.05g, ammonium nitrate 1.0g, sodium hydrogen phosphate 0.1g, regulate thickness of feed layer to be respectively 25cm, 35cm, 45cm, 55cm, 65cm, add cotton plug, with pressure cooker 121 DEG C of sterilizing 30min.By weight 10% by four strain inoculation on culture medium.After having inoculated, be put into by culture medium in incubator, cultivation temperature is 30 DEG C, and incubation time is 72h.
Result of the test shows, thickness of feed layer is in the scope of 35cm ~ 55cm, and CP difference is not remarkable, but the CP content be all significantly higher than when thickness of feed layer is 65cm, observe in process of the test: along with the increase of thickness of feed layer, the speed of growth of bacterial classification slows down, the quality variation of product simultaneously.After thickness of feed layer is greater than 55mm, there is peculiar smell in product, and its CP obviously declines simultaneously.When thickness of feed layer is 45cm, CP content arrives the highest, and improve 59.28% than unfermentable mixed substrates, crude fibre reduces 29.16%.Therefore within regulating thickness of feed layer to select 45cm aborning.See accompanying drawing 6.
Embodiment 3: apply the method Biological optimization grape pip dregs of rice of the present invention test
Employing compound strain is respectively: F67, lactic acid bacteria, bacillus subtilis, S. cervisiae; Its ratio according to volume fraction is 3:1:2:1 (in zymocyte liquid); Its optimum inoculation amount according to weight fraction is 15%; In solid-state fermentation culture medium, grape pip dregs of rice adding proportion is 80%, and wheat bran is 20%; Nitrogenous source is ammonium nitrate, and mark adding proportion is 2.0% by weight; Inorganic salts are sodium hydrogen phosphate and calcium carbonate, and mark adding proportion is 0.2% and 0.1% by weight; Best fermentation time is 72h; Best material-water ratio is 2:1; Best thickness of feed layer is 45cm.
By 5 demonstration tests, Biological optimization grape pip dregs of rice feed main nutrient composition is as follows: crude protein 18.35%, crude fibre 35.99%, acid detergent fiber 51.18%, neutral detergent fiber 59.46%, calcium 0.91%, phosphorus 0.48%, tannin content are zero.Gained CP content improves 52.83%, 51.58%, 50.89%, 49.99%, 51.34% successively than unfermentable mixed substrates; CF content in turn reduces 35.96%, 36.40%, 36.89%, 36.95%, 36.35% than unfermentable mixed substrates.True albumen average out to 13.35%, improves 90.86% than for fermented product.Basically identical with the result of case study on implementation 2; Show, optimised process of the present invention has fine stability.Under determined zymotechnique, drying primary fermentation product is burgundy look, and have strong aroma and sour fragrance, without musty, water content is about 10%.
Embodiment 4: Biological optimization grape pip dregs of rice diet effect test
Above-mentioned four compound strain Biological optimization grape pip dregs of rice are added in the diet of milk cow and poultry in proportion, measures feeding effect.Result of the test shows, fermented product has fragrant, slightly tart flavour, and color is burgundy look; Add according to 20% ratio in milk cow forage, grazing speed is obviously accelerated, and ight soil is normal, and body cell obviously reduces, fur light, and the output of milk improves; Add according to 8% ratio in poultry feed, the grazing speed of poultry is accelerated, and the speed of growth and feed conversion rate significantly improve, and intestinal beneficial bacterium significantly improves, and Escherichia coli reduce, and ight soil is normal, and feather light, has no adverse reaction.Compare with control group (not adding fermented grape seed dregs of rice feed ingredient), body weight increases by 15.23%, and feed conversion rate improves 17.98%, and feed cost reduces by 5.91%.Show, the compound strain Biological optimization grape pip dregs of rice have good palatability and feeding effect.
Compound strain of the present invention optimizes the method for grape pip nutritive value compared with other single fermenting substrate method, and the crude protein of tunning and true albumen can be made to significantly improve, and crude fibre significantly reduces; Considerably increase the nutrition content of the grape pip dregs of rice, improve nutritional utilization and be worth and safety in utilization; Technique can be widely used in the further exploitation of grape pip dregs of rice fermented product, has widened the extensive utilization in livestock and poultry diet, has had the wide prospect of marketing.
Claims (8)
1. a method for Biological optimization grape pip dregs of rice nutritive value, is characterized in that, described method includes following step:
1) raw material disposal: it is that 40 order pulverizers are pulverized that the grape pip dregs of rice and wheat bran are mixed rear granularity; Then be added to the water and make culture medium;
2) preparation of multiple and flora: make composite flora after penicillium oxalicum, lactic acid bacteria, bacillus subtilis and S. cervisiae being carried out expansion cultivation;
3) fermentation composite flora prepared being joined carry out in the grape pip dregs of rice after pulverizing and wheat bran the grape pip dregs of rice completes the optimization of grape pip dregs of rice nutritive value.
2. the method for claim 1, is characterized in that, described step 1) culture medium in the mass ratio of the grape pip dregs of rice and wheat bran be 4:1; And the grape pip dregs of rice and wheat bran concentration is in the medium 85%.
3. method as claimed in claim 1 or 2, is characterized in that, described step 1) in culture medium in be also added with nitrogenous source and inorganic salts.
4. method as claimed in claim 3, it is characterized in that, described nitrogenous source is ammonium nitrate, and its addition is in the medium 2.0%.
5. method as claimed in claim 3, it is characterized in that, described inorganic salts are sodium hydrogen phosphate and calcium carbonate, and its addition is respectively 0.2% and 0.1%.
6. the method for claim 1, is characterized in that, described step 2) mesoxalic acid mould, lactic acid bacteria, bacillus subtilis, S. cervisiae number ratio be 3 × 10
7: 1 × 10
7: 2 × 10
7: 1 × 10
7.
7. the method as described in claim 1 or 6, is characterized in that, the deposit number of described penicillium oxalicum is CGMCC No.2260.
8. the method for claim 1, is characterized in that, described step 3) in fermentation temperature be 30 DEG C, fermentation time is 72h.
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