CN104381429A - Fruit and vegetable preservative containing vine tea extract - Google Patents
Fruit and vegetable preservative containing vine tea extract Download PDFInfo
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- CN104381429A CN104381429A CN201410693074.8A CN201410693074A CN104381429A CN 104381429 A CN104381429 A CN 104381429A CN 201410693074 A CN201410693074 A CN 201410693074A CN 104381429 A CN104381429 A CN 104381429A
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Abstract
The invention discloses a fruit and vegetable preservative containing vine tea extract. The fruit and vegetable preservative is prepared from the following raw materials in parts by weight: 1-10 parts of vine tea extract, 1-5 parts of a solvent, 0.1-0.5 part of an auxiliary agent and 20-80 parts of water. The fruit and vegetable preservative disclosed by the invention can be used for preserving fruits, has the inhibition effect on a plurality of types of fruit bacteria, and especially has the remarkable effect on the preservation of litchis, longan and bananas. The fruit and vegetable preservative containing the vine tea extract provided by the invention has the advantages of high efficiency, low toxicity, and environmental friendliness and the bacteria do not easily have the resistance; and the fruit and vegetable preservative can be used as an ideal fruit preservative and can be used for prolonging the shelf life of the fruits and preventing diseases after the fruits are picked.
Description
Technical field
The invention belongs to agricultural chemicals processing and applied technical field, particularly a kind of fruit and vegetable fresh-keeping agent containing Ampelopsis grossedentata extrat.
Background technology
Vine tea, formal name used at school is ampelopsis grossdentata [Ampelopsis grossedentata (Hand-Mazz) W.T.Wang], for the wild wooden defoliation liana of one of Vitaceae (Vitaceae Michx) Ampelopsis (Ampelopsis), belong to typical class tea plant, be commonly called as the sweet tea in mountain, white tea, manna tea, white hair monkey, white tea etc., be mainly distributed in the ground such as China Hunan, Hubei, Yunnan, Guizhou, Guangdong, Guangxi, Fujian.The former plant growth of vine tea is between height above sea level 400 ~ 1300m, and concentrate or scatteredly mix in woods on hillside, wild reserves are large, distribution and growth rhythm more clear, be the wild plant resource that can directly utilize.
Vine tea contains the trace element such as iron, zinc, calcium, magnesium, potassium, sodium of the protein of needed by human body, dietary fiber, fat, carrotene, vitamin and needed by human body, Sheng, sphagitis, hypertension etc. are crossed to prevention irascibility and has effect unique, also there is clearing heat and detoxicating, the beautifying and antisenility effect of waiting for a long time, the people not high concerning sleep quality, especially can improving water flood quality.
Vine tea is medicine food dual purpose plant, is applied among the people at first as potion herbal medicine.Provinces and regions Zhuang, the Yao nationality and the Hubei the Tujia and Miao nationalities people such as China Guangxi, Hunan commonly use its young young stem and leaf and enter tea, and make tea summer, a few days does not turn sour, and has the title of " refreshing tea ", the history of existing centuries.Record according to the document such as " Chinese herbal medicine compilation ", this property of medicine slightly sweet flavor, clearing heat and detoxicating, cure mainly icteric hepatitis, cold anemopyretic, abscess of throat etc.Among the peoplely to think, generally the disease such as aphonia, heatstroke, aphthae, acute toothache, constipation, with boiling water bubble drink vine tea, curative effect is obvious; To the vine tea external application such as whitish gingival blisters, pin bifurcation eczema, washout, also there is certain curative effect.Vine tea can complete stool medicinal, main adopt cauline leaf or root, survival dose is different because processing method is different, and mill dosing is little, larger for the tea amount of taking after mixing it with water.
Longan, lichee belong to subtropical zone sapindaceous plant, and as the characteristic fruit of South China, a kind of people of enjoying like.But these Production of fruit times are short, not easily preservation and freshness, be difficult to be transported to place far away, be difficult to there is longer shelf life, make fresh-keeping concern and the attention being always subject to people of this kind of fruit storage.
At present, the antistaling agent major part on market is based on chemical preservative, and this type of antistaling agent has contaminated environment, harmful, affects fruits and vegetables taste, the shortcoming that price is high.Natural plant extracts has environmental friendliness, to human non-toxic, easily degrades, the advantage be easily accepted.Especially in today that people's living standard improves day by day, the awareness of safety of people strengthens gradually, and nontoxic plant extract antistaling agent gains great popularity.
Prior art has the relevant report of plant extracts for fruit and vegetable fresh-keeping agent, as the CN200410055506.9 antistaling agent that to disclose with various plants essential oil medicinal extract be main component; CN200510043291.3 discloses the antistaling agent prepared for primary raw material with fresh pine needle, Radix Glycyrrhizae and fresh litchi leaf; CN200710027902.4 discloses canna rhizome powder and garlic is antistaling agent prepared by raw material; CN201010231967.2 discloses using purple stem boneset extract as antistaling agent.But, using Ampelopsis grossedentata extrat as fruit and vegetable fresh-keeping agent there are no relevant report.
Summary of the invention
The object of the invention is, for chemical preservative, there is contaminated environment, harmful, affect fruits and vegetables taste, the shortcoming that price is high, provide a kind of environmentally friendly, harmless and do not affect the antistaling agent containing Ampelopsis grossedentata extrat of fruit taste on human non-toxic.Technical scheme provided by the present invention is:
Containing a fruit and vegetable fresh-keeping agent for Ampelopsis grossedentata extrat, the following raw material composition that this fruit and vegetable fresh-keeping agent is counted by weight: Ampelopsis grossedentata extrat 1 ~ 10 part, solvent 1 ~ 5 part, auxiliary agent 0.1 ~ 0.5 part, 20 ~ 80 parts, water.
As preferably, described Ampelopsis grossedentata extrat is according to made by the following step:
(1) dried vine tea is crushed to 60-200 order, obtains vine tea powder.
(2) be that 1:10 adds vine tea powder and ethanol respectively according to solid-to-liquid ratio, be then placed in water-bath 50-60 DEG C and extract 3-5 time, extract 1-2h at every turn, filter, merging filtrate, obtains vine tea crude extract;
(3) vine tea crude extract rotary evaporation will be obtained, namely obtain Ampelopsis grossedentata extrat.
As preferably, described solvent is at least one in ethanol, acetone, butanols, ethyl acetate.
As preferably, described auxiliary agent is at least one in Tween-20, polysorbate60, Tween 80, APG.
In addition, the invention also discloses the application of this fruit and vegetable fresh-keeping agent in tropical fruit (tree) lichee, longan or banana fresh-keeping.
the beneficial effect that technique scheme produces
1, fruit and vegetable fresh-keeping agent of the present invention is made by Ampelopsis grossedentata extrat, belongs to all-natural product, this product environmentally safe, and does not affect fruit-like flavour.
2, raw material vine tea wide material sources required for the present invention, easily obtain, with low cost.
Detailed description of the invention
Below in conjunction with concrete example, the present invention is further detailed explanation.
embodiment 1: Ampelopsis grossedentata extrat is tested the indoor virulence of 8 kinds of agriculture frequently seen plants disease funguses
(1) according to vine tea powder 5kg and ethanol 50kg, be then placed in water-bath 55 DEG C and extract 3 times, extract 1h at every turn, filter, merging filtrate, obtains vine tea crude extract; Vine tea crude extract rotary evaporation will be obtained again, namely obtain Ampelopsis grossedentata extrat.
(2) by 1000,500,250,125,62.5 μ gL
-1the PDA culture medium mixing that Ampelopsis grossedentata extrat 1mL and 9mL melts, pours into while hot in sterile petri dish and makes band medicine culture medium flat plate.After culture medium solidifying, putting into 1 make the one side of band mycelia be attached to media surface for examination bacterium bacterium cake (diameter is 4mm) in each culture medium plane, take sterilized water as contrast, often processes 3 times and repeats.After cultivation 72 ~ 96h, according to contrast mycelial growth situation, measure the colony growth diameter for examination bacterium by right-angled intersection method, and with following formulae discovery inhibiting rate.
Calculate the virulence regression equation of active component by DPS v7.05 software and obtain coefficient correlation (r), EC
50value and 95% confidential interval.
Table 1 Ampelopsis grossedentata extrat is to 8 kinds of agricultural disease fungal pathogens mycelial growth inhibition rates
As can be seen from Table 1, the mycelial growth of Ampelopsis grossedentata extrat to graw mold of tomato, sclerotinia rot of colza and tomato early blight bacterium 3 kinds of disease funguses has obvious inhibitory action, relative distant inhibitory action is had to the mycelial growth of Verticillium Wilt Pathogen of Eggplant, withered germ of water-melon and tobacco brown spot pathogen 3 kinds of disease funguses, not obvious to the inhibitory action of the mycelial growth of fusarium graminearum and apple anthrax bacteria 2 kinds of disease funguses, when concentration is 1000ug/mL, the mycelial growth inhibition rate of Ampelopsis grossedentata extrat to above-mentioned 2 kinds of disease funguses is respectively 59.62% and 53.33%.
embodiment 2:
Example 1 gained Ampelopsis grossedentata extrat 10 parts, ethanol 1 part, Tween-80 0.5 part, 80 parts, water, mixing, stirs, obtains translucent antistaling agent solution.Get banana 2kg and put into solution immersion 5 minutes, taking-up drains, and preserves, healthy fruit > 95% after 20 days, rate of weight loss < 0.3% at putting into 4 DEG C.
embodiment 3:
Example 1 gained Ampelopsis grossedentata extrat 20 parts, 4 parts, butanols, Tween-60 1.0 parts, 80 parts, water, stirs, and obtains translucent antistaling agent solution.Get longan 5kg and put into solution immersion 5 minutes, taking-up drains, and stores, healthy fruit > 94% after 20 days, rate of weight loss < 0.6% at putting into 4 DEG C.
embodiment 4:
Example 1 gained Ampelopsis grossedentata extrat 20 parts, 4 parts, ethyl acetate, APG 1.0 parts, 80 parts, water, stirs, and obtains transparent antistaling agent solution.Get lichee 2kg and put into solution immersion 3 minutes, taking-up drains, store at putting into 4 DEG C, separately get commercially available P applied levels process (500mg/L) in contrast, the equal > 95% of healthy fruit after 20 days, rate of weight loss < 0.3%, fruit browning index is all less than 2.0, effect and P applied levels treatment effect close.
The aforementioned description to concrete exemplary of the present invention is to illustrate and the object of illustration.These descriptions not want the present invention to be defined as disclosed precise forms, and obviously, according to above-mentioned instruction, can much change and change.The object selected exemplary embodiment and describe is to explain certain principles of the present invention and practical application thereof, thus those skilled in the art can be realized and utilize various different exemplary of the present invention and various different selection and change.Scope of the present invention is intended to limited by claims and equivalents thereof.
Claims (5)
1. containing a fruit and vegetable fresh-keeping agent for Ampelopsis grossedentata extrat, the following raw material composition that this fruit and vegetable fresh-keeping agent is counted by weight: Ampelopsis grossedentata extrat 1 ~ 10 part, solvent 1 ~ 5 part, auxiliary agent 0.1 ~ 0.5 part, 20 ~ 80 parts, water.
2. fruit and vegetable fresh-keeping agent as claimed in claim 1, it is characterized in that, described Ampelopsis grossedentata extrat is according to made by the following step:
(1) dried vine tea is crushed to 60-200 order, obtains vine tea powder.
(2) be that 1:10 adds vine tea powder and ethanol respectively according to solid-to-liquid ratio, be then placed in water-bath 50-60 DEG C and extract 3-5 time, extract 1-2h at every turn, filter, merging filtrate, obtains vine tea crude extract;
(3) vine tea crude extract rotary evaporation will be obtained, namely obtain Ampelopsis grossedentata extrat.
3. fruit and vegetable fresh-keeping agent as claimed in claim 1, it is characterized in that, described solvent is at least one in ethanol, acetone, butanols, ethyl acetate.
4. fruit and vegetable fresh-keeping agent as claimed in claim 1, it is characterized in that, described auxiliary agent is at least one in Tween-20, polysorbate60, Tween 80, APG.
5. the application of fruit and vegetable fresh-keeping agent in tropical fruit (tree) lichee, longan or banana fresh-keeping as described in as arbitrary in Claims 1 to 4.
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CN106577994A (en) * | 2017-02-28 | 2017-04-26 | 佛山市恒南医药科技有限公司 | Film preservative suitable for egg preservation, and preparation method thereof |
CN106615061A (en) * | 2017-02-28 | 2017-05-10 | 佛山市恒南医药科技有限公司 | Special freshness-preserving agent for sweet potatoes and preparation method thereof |
CN106665817A (en) * | 2017-03-15 | 2017-05-17 | 佛山市恒南医药科技有限公司 | Compound preservation method capable of prolonging storage duration of Shatang oranges |
CN106857807A (en) * | 2017-02-16 | 2017-06-20 | 佛山市恒南医药科技有限公司 | A kind of Yangshan honey peach preservative specially |
CN106857791A (en) * | 2017-02-28 | 2017-06-20 | 佛山市恒南医药科技有限公司 | A kind of low temperature controlled atmosphere coating-film fresh-keeping method that can extend egg storage life |
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CN106879730A (en) * | 2017-03-07 | 2017-06-23 | 佛山市恒南医药科技有限公司 | A kind of preservation method that can extend hair Chinese yam Storage period |
CN106889180A (en) * | 2017-02-28 | 2017-06-27 | 佛山市恒南医药科技有限公司 | A kind of preservation method that can extend sweet potato Storage period |
CN106889179A (en) * | 2017-02-16 | 2017-06-27 | 佛山市恒南医药科技有限公司 | One Plant Extracts antistaling agent, its preparation method and its application |
CN106900841A (en) * | 2017-03-07 | 2017-06-30 | 佛山市恒南医药科技有限公司 | A kind of double leaching fresh-keeping methods that can extend the banana fresh-keeping phase |
CN106900842A (en) * | 2017-03-07 | 2017-06-30 | 佛山市恒南医药科技有限公司 | A kind of Chinese herbal medicine composite banana antistaling agent |
CN106942348A (en) * | 2017-02-16 | 2017-07-14 | 佛山市恒南医药科技有限公司 | It is a kind of to extend the preservation method of Yangshan honey peach storage period |
CN106942353A (en) * | 2017-03-15 | 2017-07-14 | 佛山市恒南医药科技有限公司 | A kind of sugar orange special plant extract composite preservative |
CN107372807A (en) * | 2017-08-31 | 2017-11-24 | 广西吉朋投资有限公司 | A kind of agent keeping vegetable fresh and preparation method thereof |
CN107372788A (en) * | 2017-08-03 | 2017-11-24 | 张红霞 | A kind of preservation method that can keep ipomoea batatas quality and flavor |
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CN106577994A (en) * | 2017-02-28 | 2017-04-26 | 佛山市恒南医药科技有限公司 | Film preservative suitable for egg preservation, and preparation method thereof |
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