KR101673202B1 - A antimicrobial composition comprising scutellariae radix, schisandrae fructus, lonicerae flos, cnidi fructus and zanthoxyli fructus - Google Patents

Abstract

The present invention relates to an antimicrobial composition containing, as an active ingredient, gold, omija, goldsmith, casualties and sancho.
More particularly, the present invention relates to an antimicrobial composition containing, as an active ingredient, gold, omija, goldsmith, casualties, and sancho having a wide range of antimicrobial spectrum with low side effects due to the use of natural products and having a wide range of antibacterial spectrum.
The composition according to the present invention may be used as an antibacterial agent, a preservative, an antiseptic, a detergent, a fungicide, a detergent, a cosmetic or the like for antibacterial, sterilization, disinfection, preservation and the like.

Description

FIELD OF THE INVENTION [0001] The present invention relates to an antimicrobial composition comprising, as an active ingredient, an antimicrobial composition containing at least one compound selected from the group consisting of gold,

The present invention relates to an antimicrobial composition containing, as an active ingredient, gold, omija, goldsmith, casualties and sancho.

More particularly, the present invention relates to an antimicrobial composition containing, as an active ingredient, gold, omija, goldsmith, casualties, and sancho having a wide range of antimicrobial spectrum with low side effects due to the use of natural products and having a wide range of antibacterial spectrum.

The composition according to the present invention may be used as an antibacterial agent, a preservative, an antiseptic, a detergent, a fungicide, a detergent, a cosmetic or the like for antibacterial, sterilization, disinfection, preservation and the like.

Many products, such as foods, medicines, functional health foods, household products, and cosmetics, which are widely used in daily life, are used to prevent the change of internal properties and to preserve the antimicrobial substance Inorganic preservatives are essential.

Presently, some of the materials used as preservatives are derived from natural sources, but most depend on chemically synthesized artifacts.

Parabens, quaternium-15, imidazolidinyl urea, phenoxyethanol, and the like.

Parabens preservatives most commonly used in household products, cosmetics, and medicines are relatively less toxic than other chemical products, but like most other chemical preservatives, they cause skin allergies, skin irritation, potential for environmental hormones, And so on.

In addition, the use of the drug within the tolerance criteria is becoming a new problem due to the long-term use of the drug and the possibility of chronic chronic toxicity, cancer and mutagenesis.

In addition, as the use of antiseptic additives continues to increase in accordance with the expanded global distribution market, the need for the development of a synthetic preservative substitute that can overcome the side effects of these chemical synthetic preservatives has increased, The need for the development of natural preservatives capable of commercialization has emerged.

Accordingly, many studies have been conducted to use natural antimicrobial substances such as fatty acids, essential oils, alkaloids, flavonoids, and phytoalexin as natural preservatives.

However, in the case of natural active substances, most of them are not commercialized because of color, stability, narrow antimicrobial spectrum, problem of form, and the like. The extracts of hinokitiol, magnolia, magnolol, DF-100 and so on.

Therefore, it is necessary to develop a natural antimicrobial substance which can substitute the existing synthetic chemical antimicrobial substance and reduce the side effect which is the biggest disadvantage of the antimicrobial drug, while at the same time having a wide spectrum of antimicrobial spectrum and stability of the form.

Accordingly, the present inventors have found that the use of a mixed (complex) extract of a medicinal herb of golden, omija, gyeonghwa, casualties and sancho containing various natural antimicrobial substances as main components has excellent antimicrobial activity and broad range of antimicrobial spectrum, Or antimicrobial compositions that can be provided as preservative raw materials.

The composition according to the present invention contains, as an active ingredient, gold, omija, gingkojia, casual wear, and anthracnose. The respective materials will be briefly described below.

Scutellariae radix is the root of perennial herbaceous Scutellaria baicalensis Georgi belonging to the family Lamiaceae. It is a root of scutellaria baicalensis Georgi, , Jaundice, gastritis and so on.

It has been reported that gold contains roots containing baicalin, baicalein, wogonin, wogonoside, neobaicalein, and sitosterol. Its pharmacological effects include antimicrobial, anticancer, antiinflammatory, antihistamine, liver function protective effect, anti-hyperlipemia, Has been reported.

Schisandra (Schisandrae Fructus) is Magnolia and (Archichlamydeae) Schisandra (Schisandra chinensis Baillon) is ripe fruit.

Omija is a medicinal and edible plant which has been widely used since ancient times in food, beverage, oriental medicine, and medicine, and various processed products have been produced.

In one room, he saw the lungs and the gods and healed them, and healed them. He had been known to have calming, calming, and fever.

Schizandrins and gomisins are known to contain lignans, triterpenoids, fatty acids and organic acids. It has also been reported that antioxidant activity, antioxidant activity, antiinflammation, and antimicrobial activity are observed.

Lonicerae Flos is a flower bud of Lonicera japonica Thunberg belonging to the genus Caprifoliaceae. It is a medicinal herb that has been used in diuretics, rhinitis, detoxification, .

Lignin, lonicolin, luteolin and flavonoid compounds such as chlorogenic acid, iridoid glucosides, loganin, sweroside, secoxyloganin, vogeloside, epivogeloside and tannin and macranthoidin B, macranthoidin A, dipsacoside B, macranthoside B and macranthoside A, apigenin, quercetin, ochnaflavone, astragalin and their dihydrates have been reported.

Recent researches on gingkohwase have been reported on antibacterial, antiinflammatory, antioxidant, anticancer, immunopotentiating and liver protective effects of gingkohwa.

The casualties (Cnidi Fructus) and the Umbelliferae bee casualties ( Cnidium monieri (L). Cussion) or casualties ( Torilis japonica Decandolle) is used as a convergent antiinflammatory in oriental medicine.

Pharmacological actions such as sedation, anti-protozoal efficacy, blood coagulation and anti-inflammatory effects have been reported.

Many studies have been conducted on the major components of casualty, including torilin, a guaianetype sesquiterpene compound, which has been reported to have anti-angiogenic activity, hepatoprotective activity and testosterone 5-reductase inhibitory activity.

Torilis Fructus uses the fruit of the Conidium monnieri CUSSON in China and the fruit of the casualties ( Torilis Japonica DC.) In Korea and Japan.

Sancho (Zanthoxyli Fructus) is a genus of deciduous shrubs linden second Rutaceae (Rutaceae) Pepper Tree (Zanthoxylum piperitum De Candolle, Zanthoxylum schinifolium Siebold et Zuccarini or Zanthoxylum bungeanum Maximowicz) is a ripe fruit peel.

It has been used as a spice because of its distinctive aroma and sour taste, and has been widely used in the private sector as traditional spices and medicinal plants for a long time.

There are many names such as papaya, jiji, jinchu, jincho, sancho, bacchus, and persimmon. Many kinds of limonene, citronellal, phellandrene, sanshool and flavonoid are contained in sancho .

It also stimulates the stomach to promote metabolism, helps the menses, helps digestion, prevents desulfurization, and stops diarrhea

On the other hand, as a prior art related to the present invention, Korean Patent No. 10-0894411 describes a herbal antibacterial coating agent and a herbal antibacterial filter coated with the antibacterial coating agent.

The prior art includes at least a group selected from the group consisting of Gosam, Namgang Bow, North Ganghwa, Gobon, Baekji, Dermis, Gypsy, Suchangpo, Yeonwoo, Sagworm, Yeoju, It contains one kind of herbal medicine extract as an active ingredient.

However, the prior art has shown that Staphylococcus aureus ) or Escherichia < / RTI > E. coli ).

Also, as a prior art related to the present invention, Japanese Patent Application Laid-Open No. 10-2011-0096102 discloses an antimicrobial composition containing a natural raw material extract, a complex natural preservative, and a preparation method thereof.

The composition according to the prior art possesses a broad spectrum of antimicrobial spectrum, antimicrobial activity similar to that of conventional synthetic preservatives, and excellent antioxidant efficacy.

However, the prior art is made up of at least one extract selected from 54 kinds of natural materials, a processed extract obtained by enzymatically treating the extract, or a processed extract obtained by biotransformation of an enzyme-treated product into a specific lactic acid bacterium or a specific microorganism. There is a problem in that it is manufactured through a lot of steps and is not economical.

Also, as a prior art related to the present invention, a composition having antimicrobial activity containing a plant mixed extract is disclosed in Korean Patent Publication No. 10-2011-0125318.

The above prior art relates to a pharmaceutical composition of a plant-mixed extract containing chalkberry extract, ginger-eel extract, golden extract and hyssop cod extract having antibacterial activity as an active ingredient.

The above-mentioned prior arts have confirmed the antimicrobial activity against 9 major pathogenic microorganisms of pathogenic infection, but there is a problem that the antimicrobial activity against fungi, yeast and the like is unknown.

Also, in the prior art related to the present invention, Staphylococcus A paper on the antimicrobial activity of medicinal herbs against aureus has been searched (Non-Patent Document 1).

Although the above-mentioned literature shows 15 kinds of antimicrobial activities of Chinese medicines such as omija, gonghwa, and gold, there is only an antibacterial activity against S. aureus .

Korean Registered Patent No. 10-0894411 (Apr. 15, 2009) Korean Patent Publication No. 10-2011-0096102 (Aug. 29, 2011) Korean Patent Publication No. 10-2011-0125318 (2011.11.21)

 Choi, Jae - Yong, and Chin - Cheol. Antimicrobial Activity of Oriental Herbs against Staphylococcus aureus. Korean Journal of Plant Biotechnology, Vol. 19, No. 4, 2006.8, 491-496

A problem to be solved by the present invention is to provide an antimicrobial composition containing gold, omija, goldsmith, casualties and sancho as an active ingredient.

A problem to be solved by the present invention is to provide an antimicrobial composition having low toxicity and side effects, ensuring stability, and excellent antimicrobial activity.

A problem to be solved by the present invention is to provide an antimicrobial composition having an extensive antibacterial spectrum and being prepared and used as an antibacterial agent, a preservative, an antiseptic, a detergent, a fungicide, a detergent or a cosmetic.

The present invention aims at solving the technical problem by providing an antimicrobial composition containing gold, omija, gold silver, casualties and sancho as an active ingredient.

The present invention aims at solving the technical problem by providing an antimicrobial composition excellent in antimicrobial activity and securing stability with less toxicity and side effects.

The present invention aims at solving the technical problem by providing an antimicrobial composition having an extensive antibacterial spectrum and being prepared and used as an antibacterial agent, a preservative, an antiseptic, a detergent, a fungicide, a detergent or a cosmetic.

The antimicrobial composition according to the present invention has a lower toxicity than a chemically synthesized antimicrobial agent by using a natural product as an effective ingredient and has the effect of not causing side effects such as skin allergy induction, skin irritation, potential as an environmental hormone, and inducing resistant bacteria.

In addition, the antimicrobial composition according to the present invention can be used for the treatment of Staphylococcus aureus ) , Gram-negative bacteria Escherichia coli), Pseudomonas aeruginosa (Pseudomonas aeruginosa), hyomoryu Candida albicans (Candida albicans) and Aspergillus fungi in Braga Sicily N-Sys (Aspergillus brasiliensis ) , thus possessing a broad spectrum of antimicrobial activity.

Also, the antimicrobial composition according to the present invention can be effectively used for achieving the purpose of antibacterial, sterilizing, disinfecting, preserving, etc. in antibacterial agent, preservative, preservative, detergent, fungicide, detergent or cosmetics.

FIG. 1 is a table showing the results of measuring the antimicrobial activity of the composition according to the present invention and the materials used as a control group.

The terms and words used in the present specification and claims should not be construed as limited to ordinary or dictionary terms and the inventor may properly define the concept of the term in order to best describe its invention It should be construed as meaning and concept consistent with the technical idea of the present invention.

Therefore, the experimental examples and the reference examples described in the present specification are merely the most preferred embodiments of the present invention and are not intended to represent all of the technical ideas of the present invention. Therefore, various equivalents and variations Examples should be understood.

Example  1. Preparation of antimicrobial composition

1) Material preparation step

The composition according to the present invention is prepared from gold, omija, gold silver, casualties and sancho as active ingredients.

The gold, the omelet, the gingko gill, the casual wearer and the sancho can be used without any pretreatment, dried, frozen or cut, and preferably dried and pulverized.

2) Material extraction step

Golden, Omiza, Ganhwal, Casualties and Sancho are mixed and extracted.

The mixing ratio may be 0.5 to 1.5 parts by weight of gold, 0.5 to 1.5 parts by weight of omija, 0.5 to 1.5 parts by weight of gold silver, 0.5 to 1.5 parts by weight of casualties, and 0.5 to 1.5 parts by weight of an acid. Gyeonghwa, casualties and sancho can all be mixed in equal amounts.

Various extraction methods such as a solvent extraction method, a steam distillation method, a carbon dioxide supercritical extraction method, a steam distillation method, a microwave process extraction method, and a percolation extraction method may be used as the extraction method, and an ultrasonic extraction method may be preferably used.

A variety of extraction solvents can be selected. Extractable solvents include water, ethanol, methanol, fatty oil, glycerin, mayo, propylene glycol, ether, chloroform, petroleum ether, hexane, benzene, methylene chloride, ethyl acetate, acetone, butanol and isopropanol. Can also be set variously. Preferably 70% ethanol or methanol can be used.

Also, depending on the design conditions, the extraction may be performed a plurality of times, and in extracting a plurality of times, the solvent may be added or changed. Preferably, after the first-order ultrasonic extraction, the second-order ultrasonic extraction can be performed by adding a solvent.

3) Extraction filtration step

Filter the mixed extracts of gold, omija, gyeonghwa, casualties and sancho.

The filtration method includes a sedimentation method, a membrane separation method, a centrifugation method, a fractionation distillation method, a distribution method, and the like.

The time and temperature at the time of filtration can be varied depending on the design conditions. Preferably, a method of filtering with a filter paper may be used, and may be performed a plurality of times.

4) Concentration and drying step

The filtered extract is concentrated and dried to produce an antimicrobial composition.

Examples of the concentration method include an evaporation concentration method, a freezing concentration method, a membrane concentration method, and a drying concentration method, and various methods can be used. Preferably concentrated using a rotary vaccum evaporator. The time and temperature during the concentration process may vary depending on the design conditions.

In addition to the processes described above, filtration, concentration, extraction, and the like may be repeatedly performed in accordance with design conditions, and other processes such as fermentation and freezing may be performed before and after each process.

The extract may be concentrated, dried and powdered. Drying may be performed by a method such as hot air drying, natural drying, freeze drying and the like, preferably freeze-drying.

The prepared antimicrobial composition can be stored frozen and diluted in a solvent. In this case, the solvent may be variously used such as water, methanol, ethanol, fatty oil, glycerin, mayo, floroprene glycol, ether, chloroform, petroleum ether, hexane, benzene, methylene chloride, ethyl acetate, acetone, butanol and isopropanol Can be used by diluting with 70% ethanol.

Experimental Example  1. Antimicrobial effect and activity spectrum measurement

1-1. Preparation for experiment

1) Sample

Gold, omija, gyeonghwa, casualties, and sancho were mixed in the same amount, followed by pulverization and ultrasonic extraction. 70% ethanol (Composition A) or methanol (Composition B) was used as the solvent. After the extraction, the solution was transferred, followed by addition of a solvent to perform secondary sonication.

The second extract was combined with the first extract, filtered through a filter paper (Whatman No.), concentrated using a rotary vacuum evaporator and lyophilized. The lyophilized powder was stored frozen, diluted with 70% ethanol, and used at a concentration of 400 mg / ml.

2) Culture of the strain

The strain used for the measurement of antimicrobial activity of the composition of the present invention was Staphylococcus aureus (ATCC 6538), Escherichia coli (ATCC 8739), Gram-negative bacteria ( Pseudomonas aeruginosa) (ATCC 9027), hyomoryu Candida albicans (Candida albicans) (ATCC 10231) and the fungus is Aspergillus Bra Sicily N-Sys (Aspergillus brasiliensis) (ATCC 16404) Korea Seed Association (KCCM Korea Culture Center of Microorganisms in) .

The culture of the strain was carried out under the conditions shown in Table 1.

Strain badge Temperature Gram-positive bacteria Staphylococcus aureus ATCC 6538 Trypicase strain agar / broth 37 ℃ Gram-negative bacteria
Escherichia coli ATCC 8739 Nutrient agar / broth 37 ℃ Pseudomonas aeruginosa ATCC 9027 Trypicase strain agar / broth 37 ℃ yeast fungus Candida albicans ATCC 10231 YM agar / broth 25 ℃ Fungus Aspergillus brasiliensis ATCC 16404 Potato dextrose agar / broth 25 ℃

1-2. Experimental course

The antimicrobial activity of the antimicrobial composition according to the present invention was measured by the disk method.

Five strains were inoculated in liquid medium and S. aureus , E. coli , and P. aeruginosa were cultured at 37 ℃ and C. albicans and A. brasiliensis were cultured at 25 ℃ and then uniformly plated on solid medium, respectively.

Sterilized paper discs (Advantec, 8 ㎜) were immersed in an extract of herbal medicines with different concentrations, saturated, dried and incubated at a constant interval on a solid medium, and then the diameter of the clear zone around the disc was measured.

As a control group to compare the degree of antibacterial activity of herbal medicine extracts, three products (commercial product A: water-soluble natural preservative, commercial product B: eco XXX, commercial product C: natural herbal preservative) and chemical synthesis Methylparaben (Sigma, USA), a preservative, was purchased and used.

In addition, the same amount of water and 70% ethanol used in the antimicrobial activity test were compared and compared with a negative control in order to confirm the effect of the extract dissolving solvent.

1-3. Experiment result

FIG. 1 is a table showing the results of measuring the antimicrobial activity of the composition according to the present invention and the materials used as a control group.

Antimicrobial activity measurement results, composition A and composition B has showed a strong antibacterial activity against Escherichia coli (E. coli), showed that commercially available commercial product and a synthetic preservative methyl paraben excellent antibacterial activity than the (methylparaben).

Pseudomonas aeruginosa (P. aeruginosa) The antimicrobial activity of a similar level as the commercial products, and synthetic preservatives were about.

In particular, showed the compositions A and B, Staphylococcus aureus showed excellent antibacterial activity than commercially available commercial products for the (S. aureus), the fungus Candida albicans (C. albicans) Antimicrobial activity of a similar level to the commercial product, even.

Aspergillus way around relative to other strains loose for bra Silicate N-Sys (A. brasiliensis) but showed antimicrobial activity.

In order to investigate the effect of the dissolving solvent, it was confirmed that the same amount of water and 70% ethanol used in the experiment was immersed in the disc.

Therefore, it can be seen that the composition according to the present invention has a broad antibacterial spectrum because it has antimicrobial activity against various microorganisms such as Gram-positive bacteria, Gram-negative bacteria, yeasts and mold bacteria.

Manufacturing example  1. Manufacture of antibacterial household goods

Using the antimicrobial composition prepared according to Example 1, antimicrobial household articles can be produced with antimicrobial activity.

The antimicrobial household product according to the present invention has less side effects such as toxicity, skin allergy, skin irritation, environmental hormone, and resistant bacteria than the chemically synthesized product by using natural herbal medicines.

Further, the antimicrobial household article according to the present invention has an antimicrobial activity more efficient than a commercially available product by using an antimicrobial composition having a broad spectrum of antimicrobial spectrum against various fungi.

Therefore, it can be effectively used for achieving the purpose of antibacterial, sterilizing, disinfecting, preserving, etc. in antibacterial agent, preservative, preservative, detergent, fungicide, detergent or cosmetics.

Claims (5)

Preparing a mixture by mixing gold, omija, gingkojia, casualties and sancho at the same ratio;
Extracting the mixture with a 70% ethanol solvent or a methanol solvent to prepare a first extract;
Adding the same solvent as that of the primary extraction to the mixture after the primary extract is transferred, and then performing a secondary ultrasonic extraction to prepare a secondary extract;
Mixing the first extract and the second extract, and then filtering the mixture to prepare a filtrate;
Concentrating and freeze-drying the filtrate to prepare a powder; And
And diluting the powder with 70% ethanol to prepare an antimicrobial composition at a concentration of 400 mg / ml.
The anti-
Aspergillus brasiliensis , a gram-positive bacterium, Staphylococcus aureus , Gram-negative bacterium Escherichia coli , Pseudomonas aeruginosa , Candida albicans, and yeasts, and fungus Aspergillus brasiliensis, Wherein said antimicrobial composition has an antibacterial activity against said bacteria.
delete delete delete delete

Images