KR102450751B1 - Natural preservative composition for cosmetics containing 1,2-hexanediol and manufacturing method the same - Google Patents

Abstract

The present invention relates to a natural preservative composition for cosmetics containing hexanediol and a method for manufacturing the same, comprising a concentrated extract of Cheonmundong, a concentrated extract of grape seeds, a concentrated extract of Schisandra and hexanediol (1,2-hexanediol). As a result, unlike the prior art in which hexanediol is used alone, excellent antiseptic effect can be obtained even in a small amount by using a natural preservative composition in which a concentrated extract obtained by extracting cheonmundong, grape seed, and Schisandra asiatica having organic acid properties is mixed with hexanediol. In addition, the natural antiseptic composition of the present invention has excellent antioxidant activity and moisturizing effect, so it is easy to apply to cosmetics.

Description

Natural preservative composition for cosmetics containing 1,2-hexanediol and manufacturing method the same}

The present invention relates to a natural preservative composition for cosmetics containing hexanediol, and more particularly, unlike the prior art using hexanediol alone, a concentrated extract obtained by extracting Chunmundong, grape seed, and Schisandra asiatica, which has organic acid properties, is mixed with hexanediol. Accordingly, it relates to a natural antiseptic composition for cosmetics containing hexanediol, which exhibits excellent antiseptic effect even in a small amount, and a method for preparing the same.

Cosmetics manufacturers inevitably use preservatives in the entire process from production, distribution, and consumption to prevent contamination of their products with bacteria or mold. Preservatives are formulated in cosmetics and used for the purpose of preventing product changes by inhibiting the growth of microorganisms that contaminate cosmetics from the outside. If these preservatives are not mixed, complicated means such as aseptic manufacturing process is required to secure preservative power, limiting the content or period of use, or special manufacturing of containers, which increases the tendency to lack economic versatility. . Therefore, the use of preservatives in the manufacture of cosmetics is inevitable, and representative ingredients used as such preservatives include paraben, phenoxyethanol, BHT (dibutyl hydroxy toluene), hexanediol (1) called paraoxybenzoic acid esters. ,2-hexanediol) and the like.

In the case of paraben, there are methylparaben, ethylparaben, propylparaben, butylparaben, and the like. Among them, propylparaben and butylparaben may act as a cause of cytotoxicity and skin allergy, and may adversely affect the absorption capacity and formulation, which play a decisive role in marketability. In addition, phenoxyethanol has been used as a substitute for parabens, but the US Food and Drug Administration (FDA) has warned that this ingredient may affect the nervous system and cause vomiting, diarrhea, and respiratory depression.

BHT (dibutyl hydroxy toluene) is mainly used as an antioxidant and flavoring agent in cosmetics, which is known to cause hair loss or skin hypersensitivity reactions. In addition, hexanediol (1,2-hexanediol) is used as an antibacterial agent, a preservative auxiliary role, and a moisturizer, but it is difficult to expect a paraben-level preservative effect, and it is not classified as a preservative. to be.

Disodium EDTA, Sodium Benzoate, Polysorbate, as in the prior art 'Korea Patent Office Registration Patent No. 10-191753 Preservative Composition for Functional Wipes' containing hexanediol ), citric acid, and an antibacterial auxiliary component is further added, but the above antibacterial auxiliary component is mixed with ginseng, hwangbaek, eoseongcho, ginseng, white paper, ginseng, gyomaek, baekseonpi, samae, and sasam. The technique of being an extracted composition is known.

Most of the preservative compositions for cosmetics, including the prior art, contain a large number of artificially synthesized chemical ingredients, so that side effects such as allergies may occur when some users use cosmetics. In addition, hexanediol, which acts as a preservative, has a problem in that it is necessary to add excessively during the manufacture of cosmetics to secure the expiration date, and the viscosity decreases due to the large amount of addition.

Korean Intellectual Property Office Registered Patent No. 10-191753

The present invention has been devised to solve the above problems, and unlike the prior art using hexanediol alone, a concentrated extract obtained by extracting Chunmundong, grape seed, and Schisandra schisandra having organic acid properties is mixed with hexanediol, It is characterized by providing a natural antiseptic composition for cosmetics containing hexanediol, which exhibits an excellent antiseptic effect, and a method for preparing the same.

The above object is achieved by a natural antiseptic composition for cosmetics containing hexanediol, characterized in that it contains a concentrated extract of Cheonmundong, a concentrated extract of grape seed, a concentrated extract of Schisandra, and hexanediol (1,2-hexanediol).

Here, the natural preservative composition, with respect to 1 part by weight of the concentrated extract of Cheonmundong, 1 to 3 parts by weight of the grape seed concentrated extract, 1 to 3 parts by weight of the Schisandra concentrated extract, and 1 to 3 parts by weight of hexanediol is preferably mixed.

In addition, the concentrated extract of Cheonmundong is a concentrated extract of the bark of Cheonmundong, and it contains caffeic acid, and the concentrated extract of grape seed contains a natural oligomeric proanthocyanindin complex (OPC: oligomeric proanthocyanindin complex). desirable.

The above object is also a primary extraction step of heat extraction using distilled water and ethanol at 50 to 70 ° C., respectively, Cheonmundong, grape seeds, and Schisandra; a second extraction step of ultrasonically extracting the Cheonmundong, the grape seed, and the Schisandra in the primary extracted state to obtain a Cheonmundong extract, a grape seed extract, and a Schisandra extract; Residues in the Cheonmundong extract, the grape seed extract, and the Schisandra extract are filtered, respectively, and distilled water and ethanol in the Cheonmundong extract, the grape seed extract, and the Schisandra extract from which the residues are removed are evaporated to evaporate the Cheonmundong concentrated extract and the grape seed concentrated extract, respectively. , obtaining a concentrated extract to obtain a concentrated extract of Schisandra; A hexanediol mixing step of mixing hexanediol in a natural concentrated extract obtained by mixing the concentrated extract of Cheonmundong, the concentrated extract of grape seed, and the concentrated extract of Schisandra Schisandra; is also achieved by

As described above, according to the present invention, unlike the prior art in which hexanediol is used alone, a natural preservative composition obtained by mixing a concentrated extract extracted from cheonmundong, grape seed, and Schisandra with organic acid properties with hexanediol, A preservative effect can be obtained.

In addition, the natural antiseptic composition of the present invention has excellent antioxidant activity and moisturizing effect, so it is easy to apply to cosmetics.

1 is a flowchart of a method for preparing a natural antiseptic composition for cosmetics containing hexanediol according to an embodiment of the present invention.

Hereinafter, the technical idea of the present invention will be described in more detail with reference to the accompanying drawings. Since the accompanying drawings are merely examples shown to explain the technical idea of the present invention in more detail, the technical idea of the present invention is not limited to the form of the accompanying drawings.

1 is a flowchart of a method for preparing a natural antiseptic composition for cosmetics containing hexanediol according to an embodiment of the present invention.

The natural antiseptic composition for cosmetics containing hexanediol according to the present invention includes a concentrated extract of Cheonmundong, a concentrated extract of grape seeds, a concentrated extract of Schisandra oleifera and hexanediol. Here, the natural preservative composition is mixed with 1 part by weight of the concentrated extract of Cheonmundong, 1 to 3 parts by weight of the concentrated grape seed extract, 1 to 3 parts by weight of the concentrated extract of Schisandra, and 1 to 3 parts by weight of hexanediol. The most preferable mixing ratio is Cheonmundong concentrated extract : Grape seed concentrated extract : Schisandra concentrated extract : Hexanediol = 1:1:2:2 weight ratio.

Among these natural antiseptic compositions, Cheonmundong is a perennial grass of the Liliaceae family, and the fruit is white and contains black seeds. Cheonmundong has the meaning of a winter herb that opens the gates of heaven. When consumed, Cheonmundong is light in body and clears the mind so that it can become like a god and ascend to the sky.

When the concentrated extract of Cheonmundong extracted from Cheonmundong is applied to the skin, it can make the skin elastic and help heal skin wounds. In addition, when the skin is dry or pulled a lot, it provides a moisturizing effect, thereby obtaining skin luster and radiating the skin.

In particular, when the concentrated extract of Cheonmundong is prepared using the skin of Cheonmundong, the content of caffeic acid, which is an antioxidant active material, is high in the skin of Cheonmundong.

Grape seeds are rich in powerful antioxidants and functional ingredients called natural oligomeric proanthocyanindin complex (OPC). OPC is a complex of polyphenols that helps to remove free radicals with strong antioxidant action, prevents rapid aging and prevents certain chronic diseases. In particular, it works to maintain the elasticity of blood vessels and skin by preventing the destruction of connective tissues such as collagen and elastin.

The grape seed concentrate extract extracted from these grape seeds is used as a traditional treatment for diseases related to the heart and blood vessels, such as arteriosclerosis, high blood pressure, high cholesterol and poor blood circulation. It is also effective for nerve damage, eye damage, diabetes complications, vision loss problems such as macular degeneration, edema after injury or surgery, cancer prevention, and wound healing.

Omija is called omija because it has five flavors, and it has all five tastes: sour, sweet, bitter, salty, and spicy. Omija is distributed throughout Korea, Japan, Sakhalin, Manchuria, and China, and Korean products are evaluated as the best for medicinal purposes.

It is said that the sour and salty taste of Omija is good for the kidneys, the spicy and bitter taste protects the heart and lungs, and the sweet taste is good for the spleen and stomach. Also, in Donguibogam, Omija is said to replenish empty energy, brighten the eyes, and warm the kidneys to increase stamina. This concentrated extract of Schisandra Schisandra has a skin-constricting action to help reduce pores, and is rich in vitamins, so it increases the recovery of damaged skin, and has the effect of resolving inflammation and suppressing skin troubles.

Hexanediol (1,2-hexanediol) has the structure of a surfactant, has excellent hydrophilicity, and has emulsification properties. It is also called a natural extract preservative because of its excellent moisturizing power and resistance to bacteria. However, there is a problem that the expiration date can be secured only when excessively added in the manufacturing of cosmetics, and the viscosity is lowered due to the large amount of addition. Accordingly, the present invention can form a natural antiseptic composition in which the content of hexanediol is reduced compared to the prior art.

As described above, the method for preparing a natural preservative composition for cosmetics containing hexanediol according to an embodiment of the present invention includes a primary extraction step (S100), a secondary extraction step (S200), a step of obtaining a concentrated extract (S300), and mixing of hexanediol Step S400 is included.

The primary extraction step (S100) refers to a step of heat extraction using distilled water and ethanol at 50 to 70° C., respectively, of Cheonmundong, grape seeds, and omija.

Prepare Cheonmundong, grape seeds, and Schisandra, and extract them by heat using an extraction solvent mixed with distilled water and ethanol. At this time, it is preferable that the heat extraction temperature is 50 to 70 ° C. If the temperature is less than 50 ° C, the temperature is too low and heat extraction is not performed properly. there is

Here, the extraction solvent is preferably mixed in a weight ratio of ethanol:distilled water=80:20 to 60:40, and when it is out of the corresponding range, the amount of extraction of the antioxidant component is small, so high antioxidant activity is not expected. The most preferred extraction solvent weight ratio corresponds to ethanol:distilled water=80:20 weight ratio for Cheonmundong, ethanol:distilled water=60:40 weight ratio for grape seeds, and ethanol:distilled water=60:40 weight ratio for Schisandra.

The secondary extraction step (S200) means a step of obtaining a Cheonmundong extract, a grape seed extract, and a Schisandra extract by ultrasonically extracting each of Cheonmundong, grape seed, and Schisandra in the first extracted state.

Ultrasonic extraction is an effective method for extracting or separating physiologically active substances from plant cell walls with high extraction efficiency and short extraction time compared to other extraction methods. This can increase the efficiency of obtaining the active ingredient even for a short time extraction.

Ultrasonic-assisted extraction (UAE) refers to the process of compressing a medium by vibration and transmitting a wave as a result, and it is a method capable of delivering large energy in a short time by cavitation that occurs. . In addition, it is possible to increase the kinetic energy of the reactants located in the vicinity due to the local temperature rise due to the wave friction, and it is possible to increase the pressure by inducing the pressure increase by the ultrasonic energy impact effect to simultaneously promote the heating and mixing effect, so that the extraction efficiency is high for a short reaction time can get

The second extraction step (S200) is performed using this ultrasonic extraction method, and the first extraction step (S100) is performed by applying ultrasonic waves of 20 kHz or higher to extract Cheonmundong, grape seeds, and Schisandra, respectively. Ultrasonic extraction effectively destroys the cell wall by the resonance effect when plant cell walls exist, such as Cheonmundong, grape seed, and Schisandra, and the components in the cell wall are dissolved into the extraction solvent by cell wall destruction.

At this time, the ultrasonic extraction is performed at room temperature for 5 to 25 minutes, and the extraction solvent used for ultrasonic extraction is ethanol: distilled water = 10:90 to 40:60 weight ratio is preferably used. The amount of active ingredients that can be obtained through

In this way, Cheonmundong, grape seed, and Schisandra extract are extracted through the first extraction step (S100) and the second extraction step (S200) to obtain the Cheonmundong extract, grape seed extract, and Schisandra extract.

In the step of obtaining the concentrated extract (S300), the residues in the Cheonmundong extract, grape seed extract, and Schisandra extract are filtered, respectively, and distilled water and ethanol in the Cheonmundong extract, grape seed extract, and Schisandra extract from which the residues are removed are evaporated to evaporate the concentrated extract, respectively, It refers to the step of obtaining a grape seed concentrated extract and Schisandra concentrated extract.

Even if Cheonmundong, grape seed, and omija are extracted in liquid form using an extraction solvent during the first and second extraction steps, some residues may be present. If residues are present, impurities are mixed in the final natural preservative composition, so it is not suitable for application to cosmetics.

Therefore, it is necessary to filter the residues in the Cheonmundong extract, grape seed extract, and Schisandra extract, respectively. At this time, the residue is filtered using a glass filter to obtain a liquid state Cheonmundong extract, grape seed extract, and Schisandra extract.

Distilled water and ethanol, which are extraction solvents in the thus obtained Cheonmundong extract, grape seed extract, and Schisandra extract, are evaporated to obtain a concentrated extract of Cheonmundong, a concentrated extract of grape seed, and a concentrated extract of Schisandra. At this time, distilled water and ethanol are preferably evaporated using a vacuum rotary evaporator (rotary vacuum evaporator).

Thereafter, the method may further include the step of mixing and stirring the concentrated extract of Cheonmundong, the concentrated extract of grape seed, and the concentrated extract of Schisandra, to form a uniformly dispersed natural concentrated extract, and aging the natural concentrated extract for 24 to 48 hours.

The hexanediol mixing step (S400) refers to a step of mixing hexanediol with a natural concentrated extract obtained by mixing the concentrated extract of Cheonmundong, the concentrated extract of grape seed, and the concentrated extract of Schisandra.

After aging the natural concentrated extract, hexanediol, which is used as a preservative in existing cosmetics, is mixed. Through this, the present invention obtains a natural antiseptic composition in which a natural concentrated extract and hexanediol are mixed.

Hereinafter, embodiments of the present invention will be described in more detail.

<Example 1> Measurement of total polyphenol content

Polyphenols are antioxidants that convert free radicals into harmless components, and the higher the content of these polyphenols, the better the antioxidant action.

The total polyphenol content in the finally obtained natural preservative composition is colorimetrically quantified using the Folin-Ciocalteau method. Mix 0.25 ml of natural preservative composition and 4 ml of distilled water, mix 0.25 ml of Folin-Ciocalteau reagent diluted 1:1 with distilled water, mix for 30 seconds, and react for 5 minutes. 0.5 ml of saturated sodium carbonate solution is added thereto, left at room temperature for 30 minutes, and absorbance is measured at 725 nm using a spectrophotometer. The total polyphenol content is expressed in μm equivalent of quercetin with a standard calibration curve prepared using quercetin as a standard material.

<Example 2> Preparation of concentrated extract of Cheonmundong

After purchasing the 5-year-old Cheonmun-dong collected in Gunsan, Jeollabuk-do, it was washed with water to remove the root of the beard. Then, heat was applied so that the outer skin of Cheonmundong could be easily peeled off, and then immersed in cold water to remove the outer skin and used in this example.

Distilled water as an extraction solvent, distilled water, ethanol, and ethanol were added to 5 g of Cheonmundong peel at different mixing ratios to 40 ml each, and each was extracted once at 60° C. for 60 minutes. At this time, using a stirrer was shaken in a hot water method, and the optimal extraction conditions were confirmed through Table 1 below. Table 1 corresponds to the value by the primary extraction step (S100).

extraction solvent Total polyphenol content (mgGAE/g) 100 parts by weight of distilled water 274.97±2.84 Ethanol: Distilled water 10:90 weight ratio 266.58±4.55 Ethanol: distilled water 20:80 weight ratio 270.44±7.89 Ethanol: Distilled water 40:60 weight ratio 308.75±5.02 Ethanol: Distilled water 60:40 weight ratio 394.20±3.15 Ethanol: distilled water 80:20 weight ratio 402.97±1.44 100 parts by weight of ethanol 400.87±3.21

As shown in Table 1, it can be seen that the total polyphenol content was the highest when ethanol:distilled water was used in a weight ratio of 80:20 and extracted with a stirrer. After the first extraction step (S100) corresponding to the results of Table 1, extraction was performed by varying the extraction solvent and the ultrasound time as shown in Table 2 in order to compare the efficiency of extraction using ultrasonic waves. Ultrasonic extraction was performed at room temperature for an appropriate time, and this process corresponds to the secondary extraction step (S200).

Extraction solvent / temperature / time Total polyphenol content (mgGAE/g) Ethanol: Distilled water 80:20 weight ratio / 60 °C / stirrer 60 min 402.97±1.44 Ethanol: Distilled water 10:90 weight ratio / 20 ° C / Ultrasonic 5 min 410.85±3.01 Ethanol:distilled water 20:80 weight ratio / 20℃ / ultrasonic 10min 480.85±3.01 Ethanol:distilled water 40:60 weight ratio / 20℃ / ultrasonic 15min 503.23±5.13 Ethanol: distilled water 20:80 weight ratio / 20 ° C / ultrasonic 20 min 510.32±8.41 Ethanol: distilled water 10:90 weight ratio / 20℃ / ultrasonic 25min 505.22±6.02 Ethanol 100 parts by weight/20°C/ultrasonic 30min 512.44±7.46

In the case of the secondary extraction step (S200), it can be seen that the ultrasonic extraction of ethanol: distilled water at a weight ratio of 40:60 for 15 minutes at room temperature showed the highest total polyphenol content. At this time, the ultrasonic equipment for ultrasonic extraction was Sonics & Material Inc.'s ultrasonic generator (VCX, U.S.A).

After performing this ultrasonic extraction three times, the extracted cheonmundong extract was filtered with Whatman No. 1 filter paper and the residue was then filtered using a rotary vacuum evaporator (EYELA N-N type, aspirator A-3S, Tokyo, Japan) Concentrated under reduced pressure to obtain a concentrated extract of Cheonmundong.

<Example 3> Preparation of grape seed concentrated extract

5 g of pulverized grape seeds were extracted after adding 40 ml of distilled water as an extraction solvent, distilled water, ethanol, and ethanol at different mixing ratios, and each was extracted once at 60° C. for 60 minutes. At this time, using a stirrer was shaken in a hot water method, and the optimal extraction conditions were confirmed through Table 3 below.

extraction solvent Total polyphenol content (mgGAE/g) 100 parts by weight of distilled water 240.15±4.74 Ethanol: Distilled water 10:90 weight ratio 302.81±6.22 Ethanol: distilled water 20:80 weight ratio 370.44±2.91 Ethanol: Distilled water 40:60 weight ratio 377.26±3.78 Ethanol: Distilled water 60:40 weight ratio 395.34±7.82 Ethanol: distilled water 80:20 weight ratio 382.74±3.45 100 parts by weight of ethanol 399.31±8.54

As shown in Table 3, it can be seen that the total polyphenol content was high when ethanol:distilled water was used in a weight ratio of 60:40 and extracted with a stirrer. After the results of Table 3, extraction was performed with different extraction solvents and ultrasonication times as shown in Table 4 to compare the efficacy of ultrasonic bath extraction. Unlike the stirrer extraction, ultrasonic extraction was carried out at room temperature for an appropriate time.

Extraction solvent / temperature / time Total polyphenol content (mgGAE/g) Ethanol: Distilled water 60:40 weight ratio / 60 °C / stirrer 60 min 395.34±7.82 Ethanol: Distilled water 10:90 weight ratio / 20 ° C / Ultrasonic 5 min 511.92±4.85 Ethanol:distilled water 20:80 weight ratio / 20℃ / ultrasonic 10min 522.54±8.32 Ethanol:distilled water 40:60 weight ratio / 20℃ / ultrasonic 15min 543.78±6.24 Ethanol: distilled water 20:80 weight ratio / 20 ° C / ultrasonic 20 min 552.87±2.96 Ethanol: distilled water 10:90 weight ratio / 20℃ / ultrasonic 25min 510.86±2.18 Ethanol 100 parts by weight/20°C/ultrasonic 30min 527.33±7.64

As shown in Table 4, it can be seen that the ultrasonic extraction of ethanol: distilled water in a weight ratio of 20:80 for 15 minutes at room temperature showed the highest total polyphenol content. After performing the ultrasonic extraction three times, the extracted grape seed extract was filtered with Whatman No. 1 filter paper and the residue was then concentrated under reduced pressure using a rotary vacuum evaporator (EYELA, Tokyo, Japan) to obtain a concentrated extract from the grape seed.

<Example 4> Preparation of Schisandra concentrated extract

5 g of omija was added to 40 ml each of distilled water as an extraction solvent, distilled water, ethanol, and ethanol at different mixing ratios, and each was extracted once at 60° C. for 60 minutes. At this time, using a stirrer to shake (shaking) in a hot water method, the optimal extraction conditions were confirmed through the following Table 5.

extraction solvent Total polyphenol content (mgGAE/g) 100 parts by weight of distilled water 302.17±1.02 Ethanol: Distilled water 10:90 weight ratio 294.24±5.64 Ethanol: distilled water 20:80 weight ratio 324.21±2.65 Ethanol: Distilled water 40:60 weight ratio 338.75±5.02 Ethanol: Distilled water 60:40 weight ratio 399.34±1.85 Ethanol: distilled water 80:20 weight ratio 392.22±1.44 100 parts by weight of ethanol 386.27±8.65

As shown in Table 5, it can be seen that the total polyphenol content was high when ethanol:distilled water was mixed with a stirrer at a weight ratio of 60:40 and extracted. After the results of Table 3, extraction was performed by varying the extraction solvent and ultrasonication time as shown in Table 4 in order to compare the efficacy of ultrasonic bath extraction. Unlike the stirrer extraction, ultrasonic extraction was carried out at room temperature for an appropriate time.

Extraction solvent / temperature / time Total polyphenol content (mgGAE/g) Ethanol: Distilled water 60:40 weight ratio / 60 °C / stirrer 60 min 399.34±1.85 Ethanol: Distilled water 10:90 weight ratio / 20 ° C / Ultrasonic 5 min 321.54±3.01 Ethanol: distilled water 20:80 weight ratio / 20 °C / ultrasonic 10min 411.85±4.21 Ethanol:distilled water 40:60 weight ratio / 20℃ / ultrasonic 15min 394.26±7.64 Ethanol: distilled water 20:80 weight ratio / 20 ° C / ultrasonic 20 min 467.33±5.34 Ethanol: distilled water 10:90 weight ratio / 20 ° C / ultrasonic 25 min 492.63±4.33 Ethanol 100 parts by weight/20°C/ultrasonic 30min 490.86±2.61

As shown in Table 6, it can be seen that the ultrasonic extraction of ethanol: distilled water in a weight ratio of 10:90 for 15 minutes at room temperature showed the highest total polyphenol content. After performing this ultrasonic extraction three times, the extracted Schisandra extract was filtered with Whatman No. 1 filter paper and the residue was then concentrated under reduced pressure using a rotary vacuum evaporator (EYELA, Tokyo, Japan) to obtain a concentrated Schisandra extract.

<Example 5> Concentrated extract mixture

As shown in Examples 2 to 4, a natural concentrated extract can be obtained by mixing the concentrated extract from Cheonmundong, the concentrated extract from grape seed, and the concentrated extract from Schisandra obtained through optimal conditions. At this time, the mixing ratio of each concentrated extract is expected to have antiseptic effect due to organic acid activity, and additional functions such as antioxidant and moisturizing power are expected. mixed.

Then, 1,2-hexanediol was added to the natural concentrated extract to prepare a natural preservative composition having a weight ratio of Cheonmundong concentrated extract: grape seed concentrated extract: Schisandra concentrated extract: 1,2-hexanediol = 1:1:2:2.

The preservative efficacy of the prepared natural concentrated extract (NCC), a natural preservative composition (NHC) containing 1,2-hexanediol added to the naturally concentrated extract, and 1,2-hexanediol (HEX) was investigated. For the experiment, a natural concentrated extract (NCC) was dissolved in 50 ml of distilled water at 2 wt %, and a natural preservative composition (NHC) was dissolved in 0.5 to 2.5 wt % to confirm antioxidant activity. The results can be confirmed through Table 7.

DPPH radical scavenging degree (%) ABTS cation scavenging degree (%) 2% by weight of NCC 78.46 40.13 NHC 0.5 wt% 69.78 34.86 NHC 1 wt% 72.11 42.46 NHC 1.5 wt% 81.82 50.34 NHC 2% by weight 80.36 53.21 NHC 2.5 wt% 79.95 52.46 HEX 2% by weight 54.61 37.8

Table 7 shows the degree of scavenging of DPPH (2,2-diphenyl-1-picrylhydrazyl) radical and the degree of scavenging of ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) cations, which can confirm the antioxidant activity. Confirmed. As a result, it was confirmed that the case of using a mixture of natural concentrated extracts showed more significant results than when using 1,2-hexanediol alone. In particular, it can be seen that when 1.5% by weight of the natural preservative composition is included, the degree of radical and cation scavenging is very excellent, and through this, it is shown that the antioxidant effect is good when 1.5% by weight of the natural preservative composition is added in cosmetics.

pH 5.5 2% by weight of NCC NHC 1.5 wt% HEX 2% by weight Day 3 Germ
(Pseudomonas aeruginosa) 99.9% kill 99.9% kill 99.9% kill fungus
(Aspergillus brasiliensis) 90% dead 100% dead 99.9% kill Day 7 Germ
(Pseudomonas aeruginosa) 99.9% kill 100% dead 99.9% kill fungus
(Aspergillus brasiliensis) 90% dead 100% dead 100% dead Day 14 Germ
(Pseudomonas aeruginosa) 100% dead 100% dead 100% dead fungus
(Aspergillus brasiliensis) 100% dead 100% dead 100% dead

Table 8 shows the results of measuring the preservative power using CTFA (Cosmetic, Toiletry & Fragrance Association), a general preservative measuring method of cosmetics. In the case of the natural preservative composition, despite the lower content than when 1,2-hexanediol was used alone, 100% of fungi were killed 3 days after inoculation and 100% of bacteria were killed after 7 days.

NCC NHC HEX first 13 12 12 after 2 hours 10 11 10 after 10 hours 5 6 4

Table 9 shows the results of measuring the degree of water loss using TEWL (transepidermal water loss, TEWL), which is a numerical value representing water loss in the skin. This was measured by applying a natural concentrated extract (NCC), a natural preservative composition (NHC), and 1,2-hexanediol (HEX) to the skin to measure the degree of moisture loss through the skin. do. Accordingly, as shown in Table 9, it can be seen that the difference in the degree of moisture loss between the natural concentrated extract and the natural preservative composition is not large compared to 1,2-hexanediol, which is known to have excellent moisturizing power.

The natural concentrated extract according to the present invention contains an organic acid, and when a natural preservative composition containing such a natural concentrated extract is applied to the skin, the following mechanism can be confirmed.

When the acid (protonated acid) in the natural preservative composition receives hydrogen ions (H ⁺ ) and is absorbed through the cell membrane, the acid is dissociated and the pH is lowered. Afterwards, the cell discharges hydrogen ions and absorbs sodium ions (Na ⁺ ) into the cell to maintain the physiological pH of the cell, and the microorganism is killed in this process. In other words, the energy of microorganisms is completely consumed and killed. The bacteria killing mechanism of this organic acid preservative substitute can provide a more skin-friendly feeling than the mycelial killing mechanism that destroys the surface active type cell membrane.

That is, when the natural preservative composition of the present invention is used, the energy of microorganisms is extinguished and killed, and the user's reluctance to use is reduced compared to the surfactant. In addition, unlike the prior art in which hexanediol is used alone, excellent antiseptic effect can be obtained even with a small amount by a natural preservative composition in which a concentrated extract obtained from extracts of cheonmundong, grape seed, and Schisandra with organic acid properties is mixed with hexanediol. In addition, the natural antiseptic composition of the present invention has excellent antioxidant activity and moisturizing effect, so it is easy to apply to cosmetics.

The present invention is not limited to the above-described embodiments, and various modifications can be made without departing from the gist of the present invention as claimed in the claims.

S100: 1st extraction step
S200: secondary extraction step
S300: Concentrated extract obtaining step
S400: hexanediol mixing step

Claims (4)

Cheonmundong, grape seed, and Schisandra are heat-extracted using distilled water and ethanol at 50 to 70° C., respectively, wherein the Cheonmundong is ethanol:distilled water=80:20 weight ratio, the grape seed is ethanol:distilled water=60:40 weight ratio, the Schisandra ethanol: Distilled water = primary extraction step of heat extraction in a weight ratio of 60:40;
Cheonmundong in the primary extracted state was ultrasonically extracted at a weight ratio of ethanol:distilled water=40:60 weight ratio, the grape seed to ethanol:distilled water=20:80 weight ratio, and the omija ethanol:distilled water=10:90 weight ratio. A secondary extraction step of obtaining the extract, Schisandra extract;
Residues in the Cheonmundong extract, the grape seed extract, and the Schisandra extract are filtered, respectively, and distilled water and ethanol in the Cheonmundong extract, the grape seed extract, and the Schisandra extract from which the residues are removed are evaporated to evaporate the Cheonmundong concentrated extract and the grape seed concentrated extract, respectively. , obtaining a concentrated extract to obtain a concentrated extract of Schisandra;
A hexanediol mixing step of mixing hexanediol in a natural concentrated extract obtained by mixing the concentrated extract of Cheonmundong, the concentrated extract of grape seed, and the concentrated extract of Schisandra Schisandra; . 1 weight ratio of Cheonmundong concentrated extract, 1 weight ratio of grape seed concentrated extract, 2 weight ratio of Schisandra concentrated extract, and 2 weight ratio of hexanediol (1,2-hexanediol),
A natural antiseptic composition for cosmetics comprising hexanediol, characterized in that it is prepared through the method for preparing the natural preservative composition for cosmetics according to claim 1. 3. The method of claim 2,
The concentrated extract of Cheonmundong,
It is a concentrated extract of Cheonmundong bark, and contains caffeic acid,
The grape seed concentrate extract,
A natural preservative composition for cosmetics containing hexanediol, characterized in that it contains an oligomeric proanthocyanindin complex (OPC). delete

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