CN104381254B - A kind of sex pheromone of Grapholitha molesta synergist and preparation method thereof - Google Patents
A kind of sex pheromone of Grapholitha molesta synergist and preparation method thereof Download PDFInfo
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- CN104381254B CN104381254B CN201410503260.0A CN201410503260A CN104381254B CN 104381254 B CN104381254 B CN 104381254B CN 201410503260 A CN201410503260 A CN 201410503260A CN 104381254 B CN104381254 B CN 104381254B
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- sex pheromone
- synergist
- acetic acid
- grapholitha molesta
- terpinenyl aceatae
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Abstract
The invention belongs to agriculture field, it is proposed to a kind of sex pheromone of Grapholitha molesta synergist, it includes terpinenyl aceatae and two kinds of compounds of acetic acid, and the volume ratio of terpinenyl aceatae and acetic acid is designed as 1-4:1.The present invention also proposes preparation and the application of described sex pheromone of Grapholitha molesta synergist.Result of the test of the present invention shows, ratio is terpinenyl aceatae (α-terpinyl acetate) the 2000 μ L of 4:1 and acetic acid (acetic acid) 500 μ L mixture as synergist to improve the sex pheromone attracting ability to oriental fruit months male worm, can strengthen moth trapping best.
Description
Technical field
The invention belongs to agriculture field, be specifically related to a kind of synergist traping or kill insecticide and preparation thereof.
Background technology
Oriental fruit months Grapholitamolesta (Busck) belongs to Lepidoptera Tortricidae, is one of global fruit tree pest insect, and it is distributed widely in temperate zone and Ge great Guo district, subtropical zone.Host range is wide, causes harm hidden, and You Yitao, Prunus persicanucipersica Schneider, Fructus Mali pumilae, pears etc. are injured the most serious.It is very big that heteroecism and the hidden feature such as cause harm of larva make that it is used chemical pesticide control difficulty, and is prone to produce poisoning and pesticide residual contamination, produces with green Organic Fruit and runs counter to.
Occur it is thus desirable to a kind of effectively preventing means or method control it and cause harm.Since nineteen sixty-five oriental fruit months female worm sex pheromone is identified out, the sex pheromone compound of synthetic is widely used in the population quantity investigation of oriental fruit months, prediction, mass trapping and isotropic preventing and treating.But find in Field information, when population density is low or during other reasons, application pheromone control poor effect.This points out us to need to find some materials and lures moth ability to what improve sex pheromone, i.e. exploratory development sex pheromone synergist.
Organic volatile terpinenyl aceatae (α-terpinylacetate) and acetic acid (aceticacid), insecticide itself do not have or only have faint attracting ability, but is used to the composition of sweet-and-sour liquid or additament for trapping insects always.Both compounds are added sweet-and-sour liquid by existing research, improve the trapping capability to insect sex pheromone with sweet-and-sour liquid.But there are some to limit to when using, for instance to lure non-target insects, sweet-and-sour liquid volatile or run into rainwater and excessive, insecticide can fall into and sweet-and-sour liquid container rots due to immersion be unfavorable for qualification etc..
But both compounds individually or mixture to the potentiation of sex pheromone of Grapholitha molesta as having no report.Given this we using both compounds as potentiation class material, its potentiation to sex pheromone of Grapholitha molesta is have studied in field, show that the mixture of a certain proportion of terpinenyl aceatae and acetic acid significantly improves the sex pheromone trapping capability to oriental fruit months, and the limitation for sweet-and-sour liquid, take sustained release forms, extend lasting effect, less lure non-target pests, do not damage target pest sample.
Summary of the invention
It is an object of the invention to provide a kind of sex pheromone of Grapholitha molesta synergist.
It is another object of the present invention to the preparation method proposing described sex pheromone of Grapholitha molesta synergist.
3rd purpose of the present invention is to propose the application of described a kind of sex pheromone of Grapholitha molesta synergist.
The technical scheme realizing above-mentioned purpose of the present invention is:
A kind of sex pheromone of Grapholitha molesta synergist, including terpinenyl aceatae and two kinds of compounds of acetic acid, the volume ratio of terpinenyl aceatae and acetic acid is 1-4:1.
Preferably, the volume ratio of described terpinenyl aceatae and acetic acid is 4:1.
Terpinenyl aceatae (α-terpinylacetate) structural formula is as follows:
Chemical purity >=90% of the terpinenyl aceatae adopted in the present invention, commercially available；
The structural formula of acetic acid (aceticacid) is as follows:
Chemical purity >=99.5% of the acetic acid adopted in the present invention, commercially available.
The preparation method of sex pheromone of Grapholitha molesta synergist of the present invention, is to have the polyethylene tube ethanol purge of lid, under room temperature after ethanol volatilization totally, pricks hole with pin on the lid of polyethylene tube；Terpinenyl aceatae will be soaked with again and acetate mixture defat cotton sliver will be placed in polyethylene tube；With the aperture on ParafilmTM polyethylene tube lid, it is placed in-20 DEG C of refrigerators standby.
Further, described preparation method, is that under room temperature, ethanol volatilization is clean, and pricks the aperture having diameter to be 1mm on the lid of polyethylene centrifuge tube with pin by polyethylene centrifuge tube 95% ethanol purge that volume is 4mL 3 times；Again the defat cotton sliver being soaked with the 4cm × 1cm of terpinenyl aceatae 2000 μ L and acetic acid 500 μ L mixture is placed in polyethylene centrifuge tube；With the aperture on ParafilmTM centrifuge tube lid, it is placed in-20 DEG C of refrigerators standby.
The sex pheromone of Grapholitha molesta synergist of the present invention application in the trapping to oriental fruit months male worm.
Specifically, described application, is that sex pheromone of Grapholitha molesta synergist of the present invention is combined use with sex pheromone.
The method that the method for trapping oriental fruit months can adopt routine.Such as, when field carries out trapping experiments, the synergist that sex pheromone of Grapholitha molesta lure and the present invention propose is combined and uses, add in a lure and a polyethylene tube are fixed in the triangle trapper of insect-sticking plate, be placed in insect-sticking plate surface.On branch trapper being placed in theatre from ground 1-3 rice height.
The beneficial effects of the present invention is:
The sex pheromone of Grapholitha molesta synergist that the present invention proposes is combined use with sex pheromone of Grapholitha molesta, improves its attracting ability to oriental fruit months male worm, improves induced moth number.
Result of the test of the present invention shows, ratio is terpinenyl aceatae (α-terpinylacetate) the 2000 μ L of 4:1 and acetic acid (aceticacid) 500 μ L mixture as synergist to improve the sex pheromone attracting ability to oriental fruit months male worm, can strengthen moth trapping.
Accompanying drawing explanation
Fig. 1 be different disposal trapper lure moth dynamic curve diagram.
Detailed description of the invention
It is described in detail below in conjunction with embodiment with to the preparation of synergist of the present invention, using method and purposes, but should not be construed as limitation of the present invention.
If not specializing, the conventional means that technological means used in embodiment is well known to those skilled in the art.
Medicament: terpinenyl aceatae, purchased from ALDRICHChemistry, purity is >=90%；Acetic acid, purchased from chemical reagents corporation of traditional Chinese medicines group, purity is >=99.5%.
Embodiment 1
On May 30th, 2014, to JIUYUE 5 days, carries out the synergist synergy test to sex pheromone in Dou Dian theatre, Fangshan District of Beijing.
First by polyethylene centrifuge tube that volume is 4mL 95% ethanol purge 3 times, make ethanol volatilization clean under room temperature, bottle cap is pricked with pin the aperture having diameter to be 1mm；Compound on defat cotton sliver (4cm × 1cm) includes following 9 process:
(1) terpinenyl aceatae (α-terpinylacetate) 500 μ L is used alone,
(2) acetic acid (aceticacid) 500 μ L is used alone,
(3) mixture, terpinenyl aceatae (α-terpinylacetate) 2000 μ L and acetic acid (aceticacid) 500 μ L,
(4) mixture, terpinenyl aceatae (α-terpinylacetate) 1500 μ L and acetic acid (aceticacid) 500 μ L,
(5) mixture, terpinenyl aceatae (α-terpinylacetate) 1000 μ L and acetic acid (aceticacid) 500 μ L,
(6) mixture, terpinenyl aceatae (α-terpinylacetate) 500 μ L and acetic acid (aceticacid) 500 μ L,
(7) mixture, terpinenyl aceatae (α-terpinylacetate) 500 μ L and acetic acid (aceticacid) 1000 μ L,
(8) mixture, terpinenyl aceatae (α-terpinylacetate) 500 μ L and acetic acid (aceticacid) 1500 μ L,
(9) mixture, terpinenyl aceatae (α-terpinylacetate) 500 μ L and acetic acid (aceticacid) 2000 μ L.
The defat cotton sliver handled well is placed in centrifuge tube, is placed in-20 DEG C of refrigerators with the aperture of ParafilmTM centrifuge tube bottle mouth position standby.
When field carries out trapping experiments, by sex pheromone of Grapholitha molesta lure (purchased from Beijing Pherobio Technology Co., Ltd., production code member G013) and synergist combination, and add in being fixed in standard triangle trapper (28 × 18 × 15cm) of insect-sticking plate, it is placed in insect-sticking plate surface.It is individually placed in trapper for comparison with sex pheromone of Grapholitha molesta lure.The branch that different disposal trapper is placed in theatre from the 1.5 meters of height in ground.Each process is repeated 3 times.By trapper random arrangement in theatre, spacing 30 meters.Set time (Friday) checks and lures moth situation weekly, is cleaned out by insect-sticking plate.Calculate induced moth number, add up moth trapping.Result is in Table 1.
Table 1 different disposal trapper moth trapping comparison in difference
As can be seen from Table 1, the moth trapping obvious difference of different disposal trapper, as synergist, terpinenyl aceatae 2000 μ L+ acetic acid 500 μ L, i.e. terpinenyl aceatae: acetic acid=4:1, the potentiation of sex pheromone of Grapholitha molesta is the most obvious, it is used alone sex pheromone lure core induced moth number in conjunction with the induced moth number ratio being combined with sex pheromone after using and improves 1.4 times.
Fig. 1 uses synergist and sex pheromone to be used alone to lure moth dynamic the oriental fruit months.As seen from the figure sex pheromone combine use (terpinenyl aceatae 2000 μ L+ acetic acid 500 μ L) after synergist lure moth dynamically to be used alone sex pheromone similar, and lure the moth rush day identical, average induced moth number is significantly more than and is used alone sex pheromone.This illustrates that using potentiation lure to improve lures moth quantity, moreover it is possible to well reaction oriental fruit months occurrence dynamics.
The present invention significantly improves the sex pheromone trapping capability to oriental fruit months, moreover it is possible to well reaction oriental fruit months occurrence dynamics；And take sustained release forms, extend lasting effect, less lure non-target pests, do not damage target pest sample.
It needs to be noted, above example is merely to explanation, the present invention according to illustrating herein, can be made various correction and change by obvious those of ordinary skill in the art within the scope of the invention, and these are revised and change and also include in the scope of the present invention.
Claims (1)
1. the preparation method of a sex pheromone of Grapholitha molesta synergist, it is characterized in that, by polyethylene centrifuge tube that volume is 4mL 95% ethanol purge 3 times, under room temperature, ethanol volatilization is clean, and pricks the aperture having diameter to be 1mm on the lid of polyethylene centrifuge tube with pin；Again the defat cotton sliver being soaked with the 4cm × 1cm of terpinenyl aceatae 2000 μ L and acetic acid 500 μ L mixture is placed in polyethylene centrifuge tube；With the aperture on ParafilmTM centrifuge tube lid, it is placed in-20 DEG C of refrigerators standby.
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| CN105309464A (en) * | 2015-11-16 | 2016-02-10 | 山东省花生研究所 | Holotrichia parallela sex pheromone combined synergistic agent and preparation method thereof |
| CN108719282B (en) * | 2018-06-04 | 2021-10-15 | 山西农业大学植物保护学院(山西省农业科学院植物保护研究所) | Special attractant for preventing and controlling oriental fruit moth and preventing and controlling method |
| CN110463698B (en) * | 2019-08-19 | 2021-08-10 | 山西农业大学 | Grapholitha molesta sex pheromone synergist and preparation method thereof |
| CN113170769A (en) * | 2021-05-25 | 2021-07-27 | 岳兰菊 | Method for trapping oriental fruit moth |
| CN113841697A (en) * | 2021-11-02 | 2021-12-28 | 山西农业大学 | Efficient compound synergist of grapholitha molesta sex pheromone and trapping application |
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| JP4782860B2 (en) * | 2009-08-19 | 2011-09-28 | エステー株式会社 | Insect repellents for cereals and dry matter and pest control methods for cereals and dry matter pests |
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